Virus research最新文献

{"title":"Corrigendum to “Verteporfin is an effective inhibitor of HCMV replication” [Virus Research (2024) 1–5/199475]","authors":"Woo Young Lim , Ju Hyun Lee , Youngju Choi , Keejung Yoon","doi":"10.1016/j.virusres.2024.199523","DOIUrl":"10.1016/j.virusres.2024.199523","url":null,"abstract":"","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"351 ","pages":"Article 199523"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association study of the JAK/STAT signaling pathway with susceptibility to COVID-19 in moroccan patient and in-silico analysis of rare variants","authors":"Meriem El Houdi ,&nbsp;Hanaa Skhoun ,&nbsp;Meriem El Fessikh ,&nbsp;Reda Benmansour ,&nbsp;Fatima-Zahra El Yousfi ,&nbsp;Chaimae Nebhani ,&nbsp;Mohamed Rida Tagajdid ,&nbsp;Idriss Lahlou Amine ,&nbsp;Hicham El Annaz ,&nbsp;Rabii Ameziane El Hassani ,&nbsp;Zohra Ouzzif ,&nbsp;Redouane Abouqal ,&nbsp;Khalid Ennibi ,&nbsp;Ahmed Bouhouche ,&nbsp;Jamila El Baghdadi","doi":"10.1016/j.virusres.2024.199509","DOIUrl":"10.1016/j.virusres.2024.199509","url":null,"abstract":"<div><div>The goal of our study was to explore the association of the polymorphisms in the JAK/STAT pathway among Moroccan COVID-19 patients, using a case-control approach. Next-generation sequencing was employed to investigate the <em>IFNAR1, IFNAR2, JAK1, TYK2, STAT2,</em> and <em>IRF9</em> genes within the JAK/STAT pathway. We also performed an in silico study to examine the rare variants in this pathway. Statistical analyses were conducted using MedCalc software. Protein 3D structures were determined via the I-TASSER server, with variant structures generated using PyMOL. YASARA View allowed local 3D analysis comparing native and variant structures for pathogenic rare variants.</div><div>The study encompassed 206 COVID-19 patients, averaging 45.70 ± 12.73 years and a control group (N=118). Among the examined genes, 15 common polymorphisms and 7 rare variants were identified. Adjustment for age and gender revealed a significant association between <em>TYK2</em> p.Gly363Ser (p=0.036) and COVID-19 infection, where the GA variant exhibited protective effects (0.6361 [0.3405–1.1884], p=0.035). Additionally, <em>STAT2</em> p.Met594Ile showed an association to COVID-19 risk (p=0.042), with heterozygous GC being linked to infection (p=0.037, OR=2.7135 [0.5684 -12.9532]). Notably, <em>IFNAR1</em> p.Val168Leu mutated C allele was significantly associated with reduced susceptibility to COVID-19 severity (p=0.028, OR=0.5936 [0.3725 – 0.9461]), under the additive model (p=0.045, OR=0.626 [0.3958 – 0.9899]).</div><div>Rare variants <em>IFNAR1</em> p.Trp318Cys, p.Ser476Phe, and <em>IFNAR2</em> p.Cys271Tyr were predicted deleterious, impacting protein structure via hydrogen bond and hydrophobic interaction alterations. Burden analysis of rare variants revealed a protective cumulative effect against COVID-19 severity for <em>TYK2</em> (p=0.0013, OR=0.1438 [0.04237 – 0.4803]) under the dominant model.</div><div>This study underscores the role of genetic factors in COVID-19 susceptibility and advocates further explorations regarding functional impacts of JAK/STAT pathway rare variants.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"351 ","pages":"Article 199509"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue infection changes the expressions of CD154 and CD148 in human platelets","authors":"Sayali Vedpathak ,&nbsp;Sonali Palkar ,&nbsp;AkhileshChandra Mishra ,&nbsp;Vidya A Arankalle ,&nbsp;Shubham Shrivastava","doi":"10.1016/j.virusres.2024.199519","DOIUrl":"10.1016/j.virusres.2024.199519","url":null,"abstract":"<div><div>Platelets are essential for hemostasis and vascular integrity. Platelets recognize dengue virus through the DC-SIGN receptor. Upon pathogen recognition, platelets rapidly modulate the expression of adhesion molecules to trigger immune cell interactions and regulate the immune response. In this study, we aimed to examine the expression levels of three molecules, CD151, CD154, and CD148 on platelets in dengue patients. A significantly increased expression of CD154 and reduced expression of CD148 was observed in dengue patients compared to healthy subjects (<em>p</em> &lt; 0.0001). Moreover, a strong positive correlation between CD148 and CD41/CD61 (<em>p</em> &lt; 0.0001) was noted in dengue patients. In summary, we demonstrated the altered expressions of CD154 and CD148 on platelets in dengue patients that may play a crucial role in dengue pathogenesis.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"351 ","pages":"Article 199519"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11741917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for the replication of a plant rhabdovirus in its arthropod mite vector","authors":"Hideki Kondo ,&nbsp;Miki Fujita ,&nbsp;Paul Telengech ,&nbsp;Kazuyuki Maruyam ,&nbsp;Kiwamu Hyodo ,&nbsp;Aline Daniele Tassi ,&nbsp;Ronald Ochoa ,&nbsp;Ida Bagus Andika ,&nbsp;Nobuhiro Suzuki","doi":"10.1016/j.virusres.2024.199522","DOIUrl":"10.1016/j.virusres.2024.199522","url":null,"abstract":"<div><div>Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (<em>Brevipalpus californicus</em> s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"351 ","pages":"Article 199522"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycovirome of Diaporthe helianthi and D. gulyae, causal agents of Phomopsis stem canker of sunflower (Helianthus annuus L.)","authors":"Chien-Fu Wu ,&nbsp;Elizabeth Regedanz ,&nbsp;Febina Mathew ,&nbsp;Ruchika Kashyap ,&nbsp;Karthika Mohan ,&nbsp;Shin-Yi Lee Marzano","doi":"10.1016/j.virusres.2024.199521","DOIUrl":"10.1016/j.virusres.2024.199521","url":null,"abstract":"<div><div><em>Diaporthe gulyae</em> and <em>D. helianthi</em> cause Phomopsis stem canker, which is a yield-limiting fungal disease of sunflower (<em>Helianthus annuus</em> L.) in the United States. In this study, the mycovirus population was characterized in <em>D. gulyae</em> and <em>D. helianthi</em> using 52 and 42 isolates, respectively, that were recovered from diseased sunflower plants randomly sampled from commercial sunflower fields in the U.S. states of Minnesota, Nebraska, North Dakota, and South Dakota. Total RNA extracts depleted of rRNA from each fungus were pooled to construct one library for sequencing to obtain 20 GB per library of raw reads using a metatranscriptomics approach. Only the family <em>Mitoviridae</em> was present in both <em>Diaporthe</em> species. Twelve and nine novel viral contigs were discovered infecting <em>D. gulyae</em> and <em>D. helianthi</em>, respectively. Additionally, we detected two of the same viruses infecting <em>D. helianthi</em>, Helianthus annuus leaf-associated partitivirus 3 and 5, that were detected in a direct sunflower metatranscriptome reported before. Interestingly, <em>Qinvirus</em>, which is mostly known as a group of insect viruses, was found in a contig. An ambivirus that is rarely reported in the phylum <em>Ascomycota</em> was also discovered in this study. Besides an understanding of virome diversity, the mycovirome survey provides the first clue of biological molecules that can be further developed for antifungal purposes.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"351 ","pages":"Article 199521"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, genotype diversity, and zoonotic potential of bovine rotavirus A in Amhara National Regional State, Ethiopia: A multicenter cross-sectional study","authors":"Debasu Damtie ,&nbsp;Aschalew Gelaw ,&nbsp;Yitayih Wondimeneh ,&nbsp;Yetemwork Aleka ,&nbsp;Zewdu Siyoum Tarekegn ,&nbsp;Phillip Davis ,&nbsp;Belay Tessema ,&nbsp;Anastasia N. Vlasova","doi":"10.1016/j.virusres.2024.199504","DOIUrl":"10.1016/j.virusres.2024.199504","url":null,"abstract":"<div><div>Rotavirus A (RVA) is one of the major viral causes of acute gastroenteritis in calves globally. Bovine RVA can represent a public health concern as it is capable of zoonotic transmission. We assessed the burden, genotype distribution, and zoonotic potential of RVA among calves in Amhara National Regional State, Ethiopia.</div><div>A multi-center cross-sectional study was conducted involving a total of 266 calves. Clinical data and fecal samples were collected by trained veterinarians. Total RNA was extracted using QIAamp viral RNA mini kit and RVA was detected by One-step qRT-PCR. Amplification and Sanger sequencing of VP7 and VP4 genes were performed to determine G-types and P-types of the circulating RVA, respectively.</div><div>The prevalence of RVA among calves was 41/266 (15.4 %, 95 % CI = 11.3 %–19.5 %). The circulating G-types were G6, G8, and G10, while the circulating P-types were P[1], P[4], P[8], P[11], P[13] and P[14]. G10P[11] (37.5 %) followed by G6P[14] (18.8 %), and G6P[1] (12.5 %) were the dominant G/P combinations circulating among calves in the study area. The circulating bovine RVA strains including human-like bovine (GxP[4] and GxP[8]) and porcine-like bovine (G8P[13]) P-genotypes identified in calves were closely related to RVA strains globally reported from bovine, human, caprine, porcine, and other hosts.</div><div>Our data reveal that the prevalence of RVA in calves is significant with diverse genotypes circulating in the study area with a potential for zoonosis and/or reverse zoonosis. Hence, continuous surveillance of the circulating RVA genotypes is crucial to curb the RVA-associated morbidity and mortality in cattle and human populations.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199504"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy assessment of antiretroviral drugs against equine infectious anemia virus in vitro","authors":"Cécile Schimmich ,&nbsp;Astrid Vabret ,&nbsp;José-Carlos Valle-Casuso","doi":"10.1016/j.virusres.2024.199503","DOIUrl":"10.1016/j.virusres.2024.199503","url":null,"abstract":"<div><div>Equine infectious anemia virus (EIAV) is an equine <em>lentivirus</em> related to human immunodeficiency virus type 1 (HIV-1). Both viruses are related among the <em>Retroviridae</em> family, but their clinical manifestations are different as EIAV causes a long persistent infection with no progressive immune dysfunction in most cases. Today, no treatment is approved against EIAV, contrary to HIV-1, manageable through antiretroviral therapy, known as HAART (highly active antiretroviral therapy) or cART (combination antiretroviral therapy). No information about the efficacy of antiretroviral drugs against EIAV is available in the literature. This study evaluates the <em>in vitro</em> antiviral effect of eighteen FDA-approved antiretroviral compounds from different drug families, in an equine cells <em>in vitro</em> infection model with EIAV reference strain. Equine dermal cells, as well as equine peripheral blood mononuclear cells were treated with non-cytotoxic drug concentrations and infected with EIAV. Relative virus release in culture supernatants was assessed through relative quantification of viral RNA via RTqPCR and viral DNA comprising proviral integration in the cell genome was assessed through qPCR of infected cells, both after nucleic acid extractions. Out of eighteen tested drugs, thirteen showed a significant antiviral effect against EIAV <em>in vitro</em>, an interesting discovery showing the similarities between HIV-1 and EIAV and opening a possibility to treat equine infectious anemia to avoid the disease spread.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199503"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum","authors":"Elina Roininen ,&nbsp;Eeva Johanna Vainio ,&nbsp;Suvi Sutela ,&nbsp;Anna Poimala ,&nbsp;Muhammad Kashif ,&nbsp;Tuula Piri ,&nbsp;Jarkko Hantula","doi":"10.1016/j.virusres.2024.199467","DOIUrl":"10.1016/j.virusres.2024.199467","url":null,"abstract":"<div><div>The combined use of Heterobasidion partitiviruses 13 and 15 (HetPV13-an1 and HetPV15-pa1) is considered a promising biocontrol approach against Heterobasidion root and butt rot. In a previous study, the transmission frequency of HetPV15-pa1 was found to be higher from a double partitivirus-infected donor than from a single partitivirus-infected donor. In this study, we included a wider array of recipient isolates to assess whether the phenomenon is widespread across different host strains and conducted transmission experiments on artificial media (<em>in vitro</em>) using a total of 45 different <em>H. annosum</em> donor-recipient pairs. In addition to investigating whether double partitivirus infection improves the transmission of HetPV13-an1 and HetPV15-pa1, we examined for the first time how efficiently co-infecting ssRNA viruses are concomitantly transmitted with the partitiviruses, and whether pre-existing ssRNA viruses in the recipients affect virus transmission. Generally, the transmission rates of HetPV13-an1 and HetPV15-pa1 were high from both single partitivirus-infected and double partitivirus-infected donors to most of the <em>H. annosum</em> recipient strains, with few exceptions. However, in contrast to previous experiments, the transmission frequency was not higher from the double partitivirus-infected donors. Also, ourmiavirus was transmitted between <em>H. annosum</em> strains, but the presence of another ourmiavirus in the recipient might affect the efficacy.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199467"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild Brazilian yellow fever virus infection in Syrian hamsters (Mesocricetus auratus): Clinical and histopathological analyses","authors":"Fernanda de Oliveira Bottino ,&nbsp;Barbara Cristina Euzebio Pereira Dias de Oliveira ,&nbsp;João Paulo Rodrigues dos Santos ,&nbsp;Mariana Barata Viana Tiradentes ,&nbsp;Yuli Rodrigues Maia de Souza ,&nbsp;Tainah Silva Galdino de Paula ,&nbsp;Hyago da Silva Medeiros Elido ,&nbsp;Isabele Barbieri dos Santos ,&nbsp;Ieda Pereira Ribeiro ,&nbsp;Myrna Cristina Bonaldo ,&nbsp;Marcelo Pelajo Machado ,&nbsp;Pedro Paulo de Abreu Manso","doi":"10.1016/j.virusres.2024.199505","DOIUrl":"10.1016/j.virusres.2024.199505","url":null,"abstract":"<div><div>The Yellow Fever virus (YFV) wild-type strains studied until now have little or no ability to evade the Syrian hamster interferon antiviral response. Thus, evaluating the susceptibility of this model to new YFV isolates is paramount to aid in the understanding of their viscerotropic phenotype. To this end, Syrian hamsters were inoculated intraperitoneally with two Brazilian wild-type YFV isolates originated from dying or dead howler monkeys obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408) and Rio de Janeiro in 2019 (RJ155). The results were compared with a YFV experimental vaccine strain (17DD_exp). The main findings observed for animals infected with the PR4408 strain were progressive weight loss and persistent viremia (at least up to day seven post-infection), associated with viral RNA detection in the liver, and hepatic, splenic, and pancreatic histological alterations consistent with YF. The infection was eliminated seven days post-infection in animals inoculated with the RJ155 strain. No changes were observed for animals infected with 17DD_exp virus. The findings indicate that both Brazilian isolates are able to infect Syrian hamsters, resulting in histopathological changes compatible with the YF pathology observed in humans. Furthermore, the PR4408 strain exhibited increased virulence in this mammalian model, despite causing a non-fatal infection.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199505"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus Research: 40 years and still going strong","authors":"Ben Berkhout ,&nbsp;Esteban Domingo ,&nbsp;Nobuhiro Suzuki","doi":"10.1016/j.virusres.2024.199493","DOIUrl":"10.1016/j.virusres.2024.199493","url":null,"abstract":"","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199493"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}