Veterinary journal最新文献_第5页

Video-assisted ovarian sterilization by occluding the vascular supply with a modified Miller's knot and performing bilateral partial excision of the ovaries in the red-eared slider turtle (Trachemys scripta elegans) 视频辅助卵巢绝育，用改良的米勒结阻断血管供应并双侧部分切除红耳滑龟卵巢。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-25 DOI: 10.1016/j.tvjl.2025.106504

Luis Baselly-Cueva , Diego A. Ospina-Argüelles , Luan Sitó-Silva , Beatriz Lippe de Camillo , Kárita da Mata Fuchs , Alessandra Dhom Pimentel de Moraes , Vania Maria de Vasconselos Machado , Paulo Fernandes Marcusso , Carlos González-Zambrano , Luís F. Barbisan , David Javier Galindo , Luis Mauricio Montoya Florez , Eunice Oba

This study evaluated the efficacy of a video-assisted sterilization technique in red-eared slider turtles (Trachemys scripta elegans), using a modified Miller’s knot to occlude ovarian blood vessels, combined with bilateral partial excision of the ovaries and follicles through the prefemoral fossa. A total of 22 adult female turtles, aged between 3 and 6 years, were included. The animals originated from two facilities—11 from a wildlife research center and 11 from a zoo—but were later classified into two groups based on their reproductive condition. Group P (n = 14) comprised animals with reproductive pathologies identified by diagnostic imaging and confirmed intraoperatively, while Group H (n = 8) included clinically healthy individuals. Imaging results were used for surgical planning and postoperative monitoring. All animals underwent reversible multimodal anesthesia, and surgical times were recorded. At 90, 120, and 150 days postoperatively, second surgical interventions were performed to remove the residual ovarian tissue for histological analysis. Histopathological evaluation at 90 days revealed necrosis, atretic follicles, and inflammatory cell infiltration. At 120 days, connective tissue remodeling, inflammation, and loss of original tissue architecture were observed. By 150 days, mineralized follicular structures, tissue repair, and scar formation were evident. Immunophenotyping using a polyclonal rat anti-estrogen receptor type 2 antibody (Dako Agilent®) showed low expression, indicating reduced hormonal stimulation. These findings demonstrate that the video-assisted sterilization technique with ovarian occlusion is effective for Trachemys scripta elegans

本研究评估了视频辅助绝育技术对红耳滑龟（Trachemys scripta elegans）的效果，该技术使用改良的米勒结来闭塞卵巢血管，并结合通过股前窝切除卵巢和卵泡的双侧部分。共包括22只年龄在3至6岁之间的成年雌龟。这些动物来自两个机构——11只来自野生动物研究中心，11只来自动物园——但后来根据它们的繁殖状况被分为两组。P组（n = 14）为经影像诊断并术中确诊的生殖病变动物，H组（n = 8）为临床健康个体。影像学结果用于手术计划和术后监测。所有动物均行可逆多模态麻醉，并记录手术时间。在术后90、120和150天，进行第二次手术干预，去除卵巢残留组织进行组织学分析。90天的组织病理学检查显示坏死，卵泡闭锁，炎症细胞浸润。在120天，观察结缔组织重塑，炎症和原始组织结构的丧失。到150天，矿化的卵泡结构、组织修复和疤痕形成明显。使用多克隆大鼠抗雌激素受体2型抗体（Dako Agilent®）进行免疫分型显示低表达，表明激素刺激减少。这些结果表明，卵巢封闭的视频辅助绝育技术对秀丽隐杆线虫是有效的

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引用次数: 0

Molecular detection of Torque teno sus virus from domestic pigs in North Central, Nigeria, 2022–2023 2022-2023年尼日利亚中北部家猪转矩猪瘟病毒分子检测

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-25 DOI: 10.1016/j.tvjl.2025.106506

Mark Samson , Daniel Geofry. ThankGod , Helen Gyang. Luka , Oluwatoyin Ruth. Morenikeji , Agnes Tinuke. Laleye , Saidu Ganih. Joel , Eche Onah. Otapka , Filibus Danjuma. Dami , Dung Davou. Pam , Nathan Yakubu. Shehu , Pam Dachung. Luka

Torque teno sus virus (TTSuV) is a ubiquitous virus affecting domestic pigs and wild boars, classified into two major species: TTSuV1 and TTSuV2. In Nigeria, there is a paucity of information on TTSuV. This study investigated the prevalence, genetic diversity, and phylogenetic relationships of TTSuV genotypes in domestic pigs in Nigeria, as well as those found globally. A total of 94 (47 serum and 47 tissue) samples collected from pigs across the six geopolitical zones of Nigeria were analysed using PCR and sequencing techniques. Phylogenetic analysis was conducted to compare the sequences with those around the globe. The overall prevalence of TTSuV infection was 81 %. The Southwest zone recorded the highest prevalence (89.29 %), followed by the North Central zone (86.67 %), and the South recorded the least (33.33 %). TTSuV1 was the most common genotype, detected in 37.23 % of samples, while TTSuVk2a (15.96 %) and TTSuVk2b (27.66 %) were also prevalent. TTSuVk2b was newly reported in Nigeria, with a higher prevalence in 2023 compared to 2022. Coinfections between multiple TTSuV genotypes were common, particularly in the North Central zone, where 57.14 % of pigs were co-infected with TTSuVk2a and TTSuVk2b. The Nigerian strains of TTSuV closely clustered with global strains, indicating a high degree of genetic similarity. This suggests the role of international trade in the spread of TTSuV and provides useful molecular insights into patterns of animal movement and potential co-infections with other swine viruses. Thus, there is a need for continuous surveillance of TTSuV, particularly in regions where pig farming is prominent.

TTSuV是一种影响家猪和野猪的普遍存在的病毒，分为TTSuV1和TTSuV2两种。在尼日利亚，缺乏关于TTSuV的信息。本研究调查了TTSuV基因型在尼日利亚以及全球家猪中的流行程度、遗传多样性和系统发育关系。使用聚合酶链反应和测序技术分析了从尼日利亚六个地缘政治区域收集的94份猪样本（47份血清和47份组织）。进行了系统发育分析，将这些序列与全球的序列进行了比较。TTSuV感染的总患病率为81% %。西南地区患病率最高（89.29 %），中北部次之（86.67 %），南部最低（33.33 %）。TTSuV1是最常见的基因型，占37.23 %,TTSuVk2a（15.96 %）和TTSuVk2b（27.66 %）也普遍存在。尼日利亚新报告了TTSuVk2b，与2022年相比，2023年的流行率更高。多种TTSuV基因型之间的共感染很常见，特别是在中北部地区，57.14% %的猪同时感染TTSuVk2a和TTSuVk2b。尼日利亚菌株与全球菌株聚类密切，具有高度的遗传相似性。这表明国际贸易在TTSuV传播中的作用，并为动物运动模式和与其他猪病毒的潜在合并感染提供了有用的分子见解。因此，有必要对TTSuV进行持续监测，特别是在养猪业突出的地区。

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引用次数: 0

Development and evaluation of a nanozyme-based lateral flow assay strip for avian leukosis virus detection 基于纳米酶的禽白血病病毒横向流动检测试纸条的研制与评价

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-24 DOI: 10.1016/j.tvjl.2025.106508

Liyan Cao , Kexiong Wang , Jie Wang , Xiaoying Jin , Bin Li , Xiaomei Wang , Ying Shen , Rui Zeng , Kai Li

Avian leukosis (AL) is a common neoplastic disease in poultry caused by the Avian leukosis virus (ALV). As there are no specific drugs or vaccines for preventing and treating this disease, population purification is currently primarily achieved by culling affected individuals and selecting and breeding leukemia-free chicken flocks. To address the lack of a rapid and accurate ALV detection method, this study developed a lateral flow strip detection system based on Fe-Co nanozyme-antibody probes. Owing to the efficient signal amplification property of nanozymes, the system significantly improved detection sensitivity, with a minimum detection limit of 12.5 TCID₅₀/mL for ALV and 0.20 ng/mL for its related proteins. Furthermore, this test strip was eight times more sensitive than the commercial enzyme-linked immunosorbent assay (ELISA) group-specific antigen detection kit. Specificity verification results showed that the detection test strip only produced a positive reaction to ALV and no cross-reactions with six common avian viruses, namely Newcastle disease virus (NDV), Marek's disease virus (MDV), infectious bursal disease virus (IBDV), fowlpox virus (FPV), infectious laryngotracheitis virus (ILTV), and avian influenza virus (AIV). Additionally, the strip exhibited excellent intra- and inter-batch repeatability, with the corresponding coefficient of variation (CV) being less than 10 %. Clinical sample detection data indicated that the results of the nanozyme-based test strip were consistent with those of the commercial double-antibody sandwich ELISA detection kit, achieving a coincidence rate of up to 100 %. Collectively, the results of this study provide a novel technical method characterized by high sensitivity, high specificity, and simple operation for the on-site rapid screening of ALV.

禽白血病（AL）是由禽白血病病毒（ALV）引起的一种常见的家禽肿瘤疾病。由于没有预防和治疗这种疾病的特异性药物或疫苗，种群净化目前主要是通过扑杀受影响的个体和选择和繁殖无白血病的鸡群来实现的。为解决目前缺乏快速准确的ALV检测方法的问题，本研究开发了一种基于Fe-Co纳米酶抗体探针的侧流条带检测系统。由于纳米酶的高效信号扩增特性，该系统显著提高了检测灵敏度，对ALV的最低检测限为12.5 TCID50/mL，对其相关蛋白的最低检测限为0.20 ng/mL。此外，该试纸条的灵敏度是市售酶联免疫吸附试验（ELISA）群体特异性抗原检测试剂盒的8倍。特异性验证结果表明，该检测试纸条仅对ALV产生阳性反应，与新城疫病毒（NDV）、马立克病病毒（MDV）、传染性法氏囊病病毒（IBDV）、禽痘病毒（FPV）、传染性喉气管炎病毒（ILTV）、禽流感病毒（AIV） 6种常见禽病毒无交叉反应。此外，该条带具有良好的批内和批间重复性，变异系数（CV）小于10 %。临床样品检测数据表明，纳米酶试纸与市售双抗体夹心ELISA检测试剂盒结果一致，符合率高达100% %。本研究结果为ALV的现场快速筛选提供了一种高灵敏度、高特异性、操作简便的新技术方法。

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引用次数: 0

Dog- to- dog bite wound management – Comparison of the antiseptic efficacy of polyhexanide and hypochlorous acid with regard to reducing the use of antibiotics: A randomized clinical trial 犬对犬咬伤处理-聚己胺和次氯酸在减少抗生素使用方面的抗菌效果的比较：一项随机临床试验。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-21 DOI: 10.1016/j.tvjl.2025.106505

M. Peters , D. Eberle , S. Reese , G. Wolf , A. Meyer- Lindenberg

Therapy of dog bite wounds often includes empirical usage of antibiotics. This prospective clinical study aimed to compare the efficacy of polyhexanide and hypochlorous acid in reducing bacterial wound bio-burden and preventing the necessity for prophylactic antibiotics and to monitor the prevalence of multidrug- resistant bacteria. Thirty-four dogs with 51 bite wounds were randomly assigned to one of the treatment groups. Wounds were surgically debrided and treated with polyhexanide or hypochlorous acid. Swabs for culturing were taken three times: before and directly after debridement and directly after lavage. Veterinary patients were further divided into post-surgery care with and without prophylactic antibiotics. Wound healing until suture removal was monitored. Data were analyzed using a generalized linear model for ordinal data. Positive bacterial culture results after the first swab were obtained in 82.4 % wounds. Staphylococcus pseudintermedius was overrepresented in wounds, which later on developed complications. Neisseria species and streptococci species were overrepresented in cases of delayed wound closure. In 41.2 % of wounds multi-drug resistant bacteria could be detected. No negative effect of occurrence of multi-drug resistant bacteria on wound healing was observed. None of the compared antiseptics showed a significantly better wound decontamination. No superior antiseptic was found for wound lavage. Hypochlorous acid-based antiseptics provide the practical advantage of a shorter contact time. Prophylactic antibiotics should always be considered in severe dog bite wounds and might not be needed in low grade bite wounds.

狗咬伤的治疗通常包括经验性使用抗生素。本前瞻性临床研究旨在比较聚己胺和次氯酸在减少细菌伤口生物负荷和预防预防性抗生素必要性方面的疗效，并监测多重耐药菌的流行情况。有51处咬伤的34只狗被随机分配到一个治疗组。手术清理伤口并用聚己胺或次氯酸处理。取拭子培养3次：清创前、清创后和洗胃后。兽医患者进一步分为术后护理使用和不使用预防性抗生素。监测伤口愈合直至拆线。对有序数据采用广义线性模型进行分析。82.4%创面首次拭子细菌培养阳性。假中间葡萄球菌在伤口中被过度代表，后来出现并发症。在延迟伤口愈合的病例中，奈瑟菌和链球菌种类被过度代表。41.2%的创面检出多重耐药菌。多重耐药菌的出现对创面愈合无不良影响。没有一种比较的防腐剂显示出明显更好的伤口去污效果。未发现较好的伤口冲洗消毒剂。次氯酸基防腐剂具有较短接触时间的实际优势。在严重的犬咬伤中应始终考虑预防性抗生素，而在轻度咬伤中可能不需要。

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引用次数: 0

Comparative prognostic value of neutrophil-albumin-related ratios to predict mortality in dogs with acute pancreatitis 中性粒细胞-白蛋白相关比值预测急性胰腺炎犬死亡率的比较预后价值。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-19 DOI: 10.1016/j.tvjl.2025.106499

Alba Díaz González , José Ignacio Cristóbal Verdejo , Lorena Espadas González , Carlos Martínez Zafra , Eva María Pérez Merino

The prognostic value of hematological ratios incorporating neutrophils and albumin as key factors in the pathophysiology and outcome of acute pancreatitis (AP) in dogs needs assessment. This study compared the predictive power of the neutrophil count-to-lymphocyte ratio (NLR), neutrophil count-to-albumin ratio (NAR), neutrophil percentage-to-albumin ratio (NPAR), and NLR-to-albumin ratio (NLR-Alb) for in-hospital mortality in dogs with AP. This retrospective cohort study included 128 dogs with AP (59.37 % survivors / 40.63 % non-survivors) and 40 healthy dogs. NLR, NAR, NPAR, and NLR-Alb were calculated upon admission. Logistic regression and the area under the receiver-operating characteristic curve (AUROC) evaluated their predictive value. All markers were significantly higher in non-surviving dogs with AP than in survivors. A one-point increase in NLR, NLR-Alb, and NAR increased the mortality risk by 4 %, 12 %, and 16 %, respectively. The AUROC, sensitivity, and specificity at the cutoff for each marker were:.622, 62.75 %, and 61 % for NLR;.708, 64.7 %, and 75.3 % for NAR;.609, 78.4 %, and 44.2 % for NPAR;.649, 82.4 %, and 45.4 % for NLR-Alb. Values above their cutoffs increased the mortality risk by 2.42, 5.6, 2.87, and 3.69-fold, respectively. In conclusion, NAR can better predict outcomes in dogs with AP than NLR. NLR-Alb is also superior to NLR but exhibits low specificity in identifying non-survivors. NPAR offers no prognostic advantage over other markers. Using these markers to predict mortality may improve treatment efficiency in dogs with AP.

在狗急性胰腺炎（AP）的病理生理和预后中，中性粒细胞和白蛋白血液学比值作为关键因素的预后价值需要评估。本研究比较了中性粒细胞计数与淋巴细胞比（NLR）、中性粒细胞计数与白蛋白比（NAR）、中性粒细胞百分比与白蛋白比（NPAR）和中性粒细胞百分比与白蛋白比（NLR- alb）对AP犬住院死亡率的预测能力。这项回顾性队列研究包括128只AP犬（59.37%幸存者/ 40.63%非幸存者）和40只健康犬。入院时计算NLR、NAR、NPAR和NLR- alb。Logistic回归和受试者工作特征曲线下面积（AUROC）评估其预测价值。所有标记物在患有AP的未存活犬中均显著高于存活犬。NLR、NLR- alb和NAR每增加1个点，死亡风险分别增加4%、12%和16%。每个标记的AUROC、敏感性和特异性分别为：NLR分别为622、62.75%和61%；708、64.7%、75.3%；609例，78.4%,NPAR 44.2%；NLR-Alb分别为649、82.4%和45.4%。高于临界值的值分别使死亡风险增加2.42倍、5.6倍、2.87倍和3.69倍。总之，NAR比NLR能更好地预测AP狗的预后。NLR- alb也优于NLR，但在识别非幸存者方面特异性较低。与其他标志物相比，NPAR没有预后优势。使用这些标记物预测死亡率可以提高AP犬的治疗效率。

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引用次数: 0

Rabbit conformational predispositions to ear abnormalities: Field study of a pedigree population 兔耳部畸形的构象倾向：一个谱系群体的实地研究。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-19 DOI: 10.1016/j.tvjl.2025.106497

Maria A. Jackson , Michaela Betts , Joanna Hedley , Charlotte C. Burn

Lop-eared conformation has previously been associated with rabbit ear disease in retrospective studies of clinical populations. Here, we investigated conformational and signalment risk factors for ear abnormalities in a field study of pedigree rabbits, where ear and skull conformation could be directly observed. Ears of 435 breeder-volunteered rabbits were examined with an otoscope at British Rabbit Council shows and studs, and risk factor analysis was performed with linear and binary logistic modelling. Inter-observer reliability was Substantial–Excellent for most variables. Lop-eared rabbits had increased scores and odds for 9/11 ear abnormalities tested compared to erect-eared rabbits, including abnormal ear canal colour (P < 0.001), ear canal stenosis (P < 0.001), multiple types of aural discharge (all P ≤ 0.035), and behavioural reactivity to ear examination (P = 0.050). More brachycephalic rabbits had higher scores for ear canal stenosis (P = 0.008), whilst more dolichocephalic rabbits had higher odds of diverticulum crusts (P = 0.013). Heavier breeds had higher risks of some ear abnormalities, whilst other conformation and signalment risk factors showed contradictory results. Lop-eared rabbits were significantly predisposed to ear abnormalities suggestive of otitis externa, whilst brachycephaly appeared less important in the aetiology of otitis externa than sometimes thought. Rabbit breeders, owners, and veterinarians must be vigilant for ear disease signs, especially in lop-eared rabbits.

在对临床人群的回顾性研究中，以前曾发现垂耳构象与兔耳病有关。在这里，我们在对纯种家兔的实地研究中调查了耳朵异常的构象和信号危险因素，在那里耳朵和头骨的构象可以直接观察到。在英国兔子协会的展览和配种会上，用耳镜检查了435只饲养员自愿饲养的兔子的耳朵，并使用线性和二元logistic模型进行了风险因素分析。观察者间的信度对大多数变量来说都是极好的。与竖耳兔相比，垂耳兔在9/11耳部异常测试中的得分和几率更高，包括耳道颜色异常(P

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引用次数: 0

Postmortem analysis of the causes of perinatal calf mortality on dairy farms 奶牛场围产期小牛死亡原因的尸检分析。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-17 DOI: 10.1016/j.tvjl.2025.106498

Dawid Król , Tadeusz Stefaniak , Wojciech Nowacki , Stanisław Dzimira , Zenon Sołtysiak , John F. Mee , Paulina Jawor

Bovine perinatal mortality (BPM) is a significant concern in dairy production owing to its economic and welfare implications. The objective of this study was to identify and classify the causes of perinatal calf mortality in Polish dairy farms using a clinicopathological investigative model. A total of 121 full-term calves (≥260 days gestation) that died before, during, or within 6 h of birth were collected from 29 farms. Each case underwent a detailed anamnesis, dam examination, and standardized necropsy. Based on the clinical, pathological, and anamnestic data, each case was assigned to one of the eight cause-of-death (COD) categories. Multinomial logistic regression and ANOVA were used to evaluate the associations between factors such as birth weight, calving difficulty, pregnancy length, foetal sex, and maternal parity and COD. Dystocia-related COD accounted for 47.1 % of all cases, with traumatic calving (traumotocia) being the most common subcategory. Other diagnosed causes included multifactorial origins (6.6 %), foetal malformations and intrauterine growth retardation (5.8 %), eutoxia (5.0 %), premature placental separation (1.7 %), anaemia (1.7 %), and infection (0.8 %). In 31.4 % of cases, no definitive cause could be determined. Dystocia was associated with significantly higher calf birth weight and longer gestation. Male calves had lower odds of dystocia than unexplained cases. Dystocia, particularly traumotocia, was the leading cause of BPM in dairy herds. This standardized clinicopathological investigative model can significantly improve diagnostic rates, although a notable proportion of cases remains unexplained. Enhancing perinatal outcomes will require improved calving management, judicious use of calving aids, and broader diagnostic support including laboratory testing.

牛围产期死亡率（BPM）是乳品生产中一个重要的问题，因为它的经济和福利影响。本研究的目的是确定和分类的原因围产期小牛死亡在波兰奶牛场使用临床病理调查模型。从29个农场收集了121头在出生前、出生中或出生后6小时内死亡的足月犊牛（妊娠≥260天）。每个病例都进行了详细的回忆、水坝检查和标准化的尸检。根据临床、病理和记忆数据，每个病例被划分为8个死因（COD）类别之一。采用多项logistic回归和方差分析来评估出生体重、产犊困难、妊娠长度、胎儿性别、产妇胎次和COD等因素之间的关系。难产相关的COD占所有病例的47.1%，外伤性产裂（创伤性产裂）是最常见的亚类。其他诊断的病因包括多因素起源（6.6%）、胎儿畸形和宫内生长迟缓（5.8%）、自体中毒（5.0%）、胎盘早分离（1.7%）、贫血（1.7%）和感染（0.8%）。31.4%的病例无法确定确切的病因。难产与犊牛出生体重显著增高和妊娠期延长有关。与原因不明的病例相比，雄性小牛难产的几率更低。难产，尤其是外伤性难产，是奶牛群发生BPM的主要原因。这种标准化的临床病理调查模型可以显著提高诊断率，尽管仍有相当比例的病例无法解释。提高围产期结局将需要改进产犊管理，明智地使用产犊辅助工具，以及包括实验室检测在内的更广泛的诊断支持。

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引用次数: 0

An innovative three-dimensional skin model for advancing canine atopic dermatitis research 推进犬特应性皮炎研究的创新三维皮肤模型。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-17 DOI: 10.1016/j.tvjl.2025.106500

Bo Chen, Ronald Francis Slocombe, Oluwadamilola Samuel Omotainse, Mirjana Bogeski, Smitha Rose Georgy

Atopic dermatitis is a chronic, inflammatory, and pruritic skin disease affecting both humans and animals. The pathogenesis of Canine Atopic Dermatitis (CAD) involves a complex interplay of genetic factors, such as skin barrier defects and immune dysregulation, as well as environmental triggers. Identifying the role of individual factors in CAD progression through clinical observational studies in atopic dogs remains challenging. This study aimed to develop an in vitro organoid model that accurately recapitulates the morphological and molecular characteristics of CAD. Canine Primary Epidermal Organoids (cPEOs) were cultured from normal canine keratinocytes and treated with Th2 cytokines (IL-4, IL-13) and TNF-α to mimic atopic skin. Morphological changes in the organoids were assessed through histological analysis and transmission electron microscopy. Molecular markers, including keratins 5 and 10, filaggrin, involucrin, loricrin, tight junction proteins, Ki67 (proliferation), and caspase-3 (apoptosis), were evaluated via RT-qPCR and/or immunostaining. Cytokine treatment induced morphological and molecular characteristics in cPEOs reminiscent of CAD, including epidermal spongiosis and reduced suprabasal keratinocyte differentiation. Th2 cytokines increased keratinocyte proliferation while TNF-α appeared to induce cellular apoptosis within the organoid model. Since these organoids effectively mimic CAD characteristics, they can serve as a valuable model system to delineate the effects of multiple factors involved in the pathogenesis of this condition. Furthermore, this organoid platform could be utilized to identify novel therapeutic approaches and test the efficacy of potential novel treatments.

特应性皮炎是一种影响人类和动物的慢性、炎症性和瘙痒性皮肤病。犬特应性皮炎（CAD）的发病机制涉及遗传因素的复杂相互作用，如皮肤屏障缺陷和免疫失调，以及环境触发。通过对特应性犬的临床观察研究确定个体因素在CAD进展中的作用仍然具有挑战性。本研究旨在建立一个体外类器官模型，准确再现CAD的形态和分子特征。从正常犬角质形成细胞中培养犬原发性表皮类器官（cPEOs），并用Th2细胞因子（IL-4、IL-13）和TNF-α处理以模拟特应性皮肤。通过组织学分析和透射电镜观察类器官的形态学变化。通过RT-qPCR和/或免疫染色评估分子标记，包括角蛋白5和10、聚丝蛋白、天青蛋白、氯丙氨酸、紧密连接蛋白、Ki67（增殖）和caspase-3（凋亡）。细胞因子治疗诱导cpeo的形态和分子特征与CAD相似，包括表皮海绵状病和基底上角化细胞分化减少。在类器官模型中，Th2细胞因子增加角质细胞增殖，而TNF-α似乎诱导细胞凋亡。由于这些类器官有效地模拟了CAD的特征，它们可以作为一个有价值的模型系统来描述这种疾病发病机制中涉及的多种因素的影响。此外，这种类器官平台可用于识别新的治疗方法和测试潜在的新治疗方法的疗效。

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引用次数: 0

Incidence and risk factors of surgical site infection in dogs undergoing laparoscopy ovariectomy. Multicentric prospective cohort study 腹腔镜卵巢切除术犬手术部位感染的发生率及危险因素。多中心前瞻性队列研究。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-16 DOI: 10.1016/j.tvjl.2025.106496

M. Fuertes-Recuero , J. Espinel Rupérez , M. Abal-Flores , G. Gonzalez-Matellano , M. Gardoqui-Arias , A. Rubio Guivernau , A. Fraile-Fernandez , D. Carrero Escolar , C. Sánchez-Collado , D. Calzado-Barranco , J.C. Fontanillas-Pérez , G. Ortiz-Diez

Canine laparoscopic ovariectomy has become more popular due to several advantages including smaller incisions and faster recovery. However, surgical site infections (SSIs) remain a common complication that increases morbidity and mortality. The aim of this study was to describe the incidence of SSI and identify the factors associated with development of SSI in dogs undergoing laparoscopic ovariectomy. A multicentre, prospective cohort study was performed, including 208 female dogs undergoing surgery at seven veterinary hospitals in Spain between January 2022 and September 2023. SSI was diagnosed according to Centers for Disease Control and Prevention (CDC) criteria, with active surveillance consisting of clinical evaluations on postoperative days 5–7 and 10–12, and follow-up telephone conversation at 30 days. SSIs were identified in 15 dogs (7.2 %) of which 12 were classified as superficial and 3 as deep infections. Duration of Surgery (P < 0.001) and Elizabethan collar (P = 0.003) were identified as risk factors of SSI. The incidence of SSI was identified in 7.2 % (IC 95 %: 4.1 %-11.6 %) of dogs and it was associated with surgical time exceeding 46 min and improper use of the Elizabethan collar. Prospective, standardised studies should test whether collar use and shorter surgical time reduce SSI after laparoscopy ovariectomy. Therefore, shorter surgical times and correct use of buster collar could reduce the incidence of SSI.

由于切口小、恢复快等优点，犬腹腔镜卵巢切除术越来越受欢迎。然而，手术部位感染（ssi）仍然是增加发病率和死亡率的常见并发症。本研究的目的是描述腹腔镜卵巢切除术犬的SSI发生率，并确定与SSI发展相关的因素。进行了一项多中心前瞻性队列研究，包括2022年1月至2023年9月期间在西班牙七家兽医医院接受手术的208只母狗。根据疾病控制和预防中心（CDC）的标准诊断SSI，积极监测包括术后5至7天和10至12天的临床评估，以及30天的随访电话交谈。15只狗（7.2%）被鉴定出ssi，其中12只被分类为浅表感染，3只被分类为深部感染。手术时间(P

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Comment on “Antimicrobial use practices among companion animal veterinarians in Hong Kong” 评论“香港伴侣动物兽医使用抗微生物药物的情况”。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-11-15 DOI: 10.1016/j.tvjl.2025.106493

S.Dhanya Dedeepya MD , Vaishali Goel , Nivedita Nikhil Desai

引用次数: 0