Pub Date : 2026-02-01Epub Date: 2026-01-28DOI: 10.1016/j.tvjl.2026.106572
Andrea Corda, Giovanni Pietro Burrai, Claudia Tamponi, Francesca Corda, Plamena Pentcheva, Marco Pau, Paola Nicolussi, Mauro Scala, Marta Polinas, Lia Cavallo, Pamela Zeinoun, Muhammad Furqan Arshad, Francesca Nonnis, Maria Luisa Pinna Parpaglia, Antonio Varcasia, Antonio Scala
Hepatic fasciolosis represents a major health and production concern in sheep farming. Although ultrasonography has been used to evaluate fasciolosis in ruminants, the feasibility of a rapid ultrasonographic protocol performed in standing sheep under field conditions has not been specifically described. This study aimed to provide a preliminary, descriptive evaluation of the feasibility of a fast on-farm ultrasonographic protocol for the assessment of hepatic alterations in sheep with suspected chronic fasciolosis. Sixteen Sarda sheep with Fasciola hepatica fecal eggs count > 100 eggs per gram underwent clinical examination, hematological tests, and liver ultrasonography. Three sheep were slaughtered, and their livers were examined by gross pathology and histopathology. The median duration of the ultrasonographic examination was 2.5 min per animal. Recurrent ultrasonographic features included heterogeneous parenchymal echotexture and echogenicity (87.5 %), irregular diaphragmatic liver surface (68.8 %), moderate to severe bile duct dilatation (50 %), and thickened gallbladder walls (56 %). In a subset of animals (37.5 %), intraluminal structures compatible with adult flukes were visualized within the biliary tract. Pathological and histological findings in the necropsied animals were consistent with the ultrasonographic features observed in vivo. This study provides preliminary descriptive evidence that a rapid hepatic ultrasonography protocol is feasible under field conditions in standing sheep with suspected chronic fasciolosis. Further prospective studies are required to clarify the diagnostic performance and clinical utility of ultrasonography in ovine fasciolosis.
{"title":"Feasibility of a rapid on-farm hepatic ultrasonography protocol in sheep with suspected chronic fasciolosis.","authors":"Andrea Corda, Giovanni Pietro Burrai, Claudia Tamponi, Francesca Corda, Plamena Pentcheva, Marco Pau, Paola Nicolussi, Mauro Scala, Marta Polinas, Lia Cavallo, Pamela Zeinoun, Muhammad Furqan Arshad, Francesca Nonnis, Maria Luisa Pinna Parpaglia, Antonio Varcasia, Antonio Scala","doi":"10.1016/j.tvjl.2026.106572","DOIUrl":"10.1016/j.tvjl.2026.106572","url":null,"abstract":"<p><p>Hepatic fasciolosis represents a major health and production concern in sheep farming. Although ultrasonography has been used to evaluate fasciolosis in ruminants, the feasibility of a rapid ultrasonographic protocol performed in standing sheep under field conditions has not been specifically described. This study aimed to provide a preliminary, descriptive evaluation of the feasibility of a fast on-farm ultrasonographic protocol for the assessment of hepatic alterations in sheep with suspected chronic fasciolosis. Sixteen Sarda sheep with Fasciola hepatica fecal eggs count > 100 eggs per gram underwent clinical examination, hematological tests, and liver ultrasonography. Three sheep were slaughtered, and their livers were examined by gross pathology and histopathology. The median duration of the ultrasonographic examination was 2.5 min per animal. Recurrent ultrasonographic features included heterogeneous parenchymal echotexture and echogenicity (87.5 %), irregular diaphragmatic liver surface (68.8 %), moderate to severe bile duct dilatation (50 %), and thickened gallbladder walls (56 %). In a subset of animals (37.5 %), intraluminal structures compatible with adult flukes were visualized within the biliary tract. Pathological and histological findings in the necropsied animals were consistent with the ultrasonographic features observed in vivo. This study provides preliminary descriptive evidence that a rapid hepatic ultrasonography protocol is feasible under field conditions in standing sheep with suspected chronic fasciolosis. Further prospective studies are required to clarify the diagnostic performance and clinical utility of ultrasonography in ovine fasciolosis.</p>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":" ","pages":"106572"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146094191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01DOI: 10.1016/j.tvjl.2026.106570
Sara Pérez-Fuentes , Elena Moreno-Grúa , Asunción Muñoz-Silvestre , Joan M. Rosell , Alberto Arnau-Bonachera , David Viana , Laura Selva , Juan Manuel Corpa
Staphylococcus aureus is a major pathogen in commercial rabbit farming, causing significant economic damage and animal welfare concerns. Staphylococcosis have been associated with a limited number of clones, particularly the highly virulent ST121 and the low-virulence ST96. However, in recent years, veterinarians and rabbit producers have reported increasingly virulent and persistent outbreaks of S. aureus in rabbit farms across Portugal and Spain. These outbreaks may reflect the evolution of novel clones with distinct epidemiological and pathogenic profiles, facilitated by the species’ inherent genomic versatility. This study aimed to characterise S. aureus isolates through genotyping, geographical mapping, whole-genome sequencing, and in vivo virulence assessment. A total of 200 isolates were analysed, revealing the predominance of ST121 (27 %), ST2855 (20.5 %), and ST3764 (16 %), with ST2855 and ST3764 representing emerging clones with wide regional distribution. Genomic analysis identified differences in virulence genes and mobile genetic elements, including the presence of enterotoxin clusters, bbp, and type VII secretion system components in CC121 strains, which were absent in CC96. Despite genetic changes potentially linked to increased virulence, in vivo assays showed that ST2855 and ST398 strains were unable to establish infection under experimental conditions, while ST3764 strains exhibited infectivity comparable to ST121.
{"title":"Molecular epidemiology and virulence determinants of emerging Staphylococcus aureus clones in farmed rabbits","authors":"Sara Pérez-Fuentes , Elena Moreno-Grúa , Asunción Muñoz-Silvestre , Joan M. Rosell , Alberto Arnau-Bonachera , David Viana , Laura Selva , Juan Manuel Corpa","doi":"10.1016/j.tvjl.2026.106570","DOIUrl":"10.1016/j.tvjl.2026.106570","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is a major pathogen in commercial rabbit farming, causing significant economic damage and animal welfare concerns. Staphylococcosis have been associated with a limited number of clones, particularly the highly virulent ST121 and the low-virulence ST96. However, in recent years, veterinarians and rabbit producers have reported increasingly virulent and persistent outbreaks of <em>S. aureus</em> in rabbit farms across Portugal and Spain. These outbreaks may reflect the evolution of novel clones with distinct epidemiological and pathogenic profiles, facilitated by the species’ inherent genomic versatility. This study aimed to characterise <em>S. aureus</em> isolates through genotyping, geographical mapping, whole-genome sequencing, and <em>in vivo</em> virulence assessment. A total of 200 isolates were analysed, revealing the predominance of ST121 (27 %), ST2855 (20.5 %), and ST3764 (16 %), with ST2855 and ST3764 representing emerging clones with wide regional distribution. Genomic analysis identified differences in virulence genes and mobile genetic elements, including the presence of enterotoxin clusters, <em>bbp</em>, and type VII secretion system components in CC121 strains, which were absent in CC96. Despite genetic changes potentially linked to increased virulence, <em>in vivo</em> assays showed that ST2855 and ST398 strains were unable to establish infection under experimental conditions, while ST3764 strains exhibited infectivity comparable to ST121.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106570"},"PeriodicalIF":3.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-30DOI: 10.1016/j.tvjl.2026.106573
Esther Palomino Lago , Scott J. Roberts , Isabel R. Orriss , Ellison S. Lumsden , Deborah J. Guest
Bone is a dynamic tissue that is maintained through the co-ordination of bone resorption and bone formation. An imbalance of these processes can lead to bone disease. In vitro studies of osteoblasts can help to understand bone formation, but primary cells have a limited lifespan in culture. Herein, we report the successful generation of equine immortalized osteoblasts through the stable overexpression of human telomerase reverse transcriptase (hTERT) and Simian virus 40 (SV40) large T-antigen in osteoblasts isolated from trabecular bone taken from the third metacarpal of a two-year-old Thoroughbred horse. Primary osteoblasts displayed limited proliferation in culture, a decrease in the expression of osteogenic-associated genes and alkaline phosphatase activity with increasing passage and a failure to survive and produce a mineralised matrix after 21 days of osteogenic culture at high passage. In contrast, immortalized equine osteoblasts could be expanded for over 50 passages while retaining osteogenic gene expression, high alkaline phosphatase activity, a normal karyotype and the ability to produce a mineralised matrix after osteogenic culture. The immortalized equine osteoblasts therefore constitute a useful in vitro model to study equine bone formation.
{"title":"Generation and characterization of an immortalized equine osteoblast cell line","authors":"Esther Palomino Lago , Scott J. Roberts , Isabel R. Orriss , Ellison S. Lumsden , Deborah J. Guest","doi":"10.1016/j.tvjl.2026.106573","DOIUrl":"10.1016/j.tvjl.2026.106573","url":null,"abstract":"<div><div>Bone is a dynamic tissue that is maintained through the co-ordination of bone resorption and bone formation. An imbalance of these processes can lead to bone disease. <em>In vitro</em> studies of osteoblasts can help to understand bone formation, but primary cells have a limited lifespan in culture. Herein, we report the successful generation of equine immortalized osteoblasts through the stable overexpression of human telomerase reverse transcriptase (hTERT) and Simian virus 40 (SV40) large T-antigen in osteoblasts isolated from trabecular bone taken from the third metacarpal of a two-year-old Thoroughbred horse. Primary osteoblasts displayed limited proliferation in culture, a decrease in the expression of osteogenic-associated genes and alkaline phosphatase activity with increasing passage and a failure to survive and produce a mineralised matrix after 21 days of osteogenic culture at high passage. In contrast, immortalized equine osteoblasts could be expanded for over 50 passages while retaining osteogenic gene expression, high alkaline phosphatase activity, a normal karyotype and the ability to produce a mineralised matrix after osteogenic culture. The immortalized equine osteoblasts therefore constitute a useful <em>in vitro</em> model to study equine bone formation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106573"},"PeriodicalIF":3.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-23DOI: 10.1016/j.tvjl.2026.106571
Dana Badura , Annette Lorch , Andrea Urgibl-Bauer , Antonia Kreuzer , Ingrid Lorenz , Yury Zablotski , Florian M. Trefz
Strong ion (metabolic) acidosis, dehydration, and electrolyte imbalances are common in neonatal calves with diarrhoea. Balanced crystalloids may provide advantages for rehydration purposes in those animals due to their polyionic composition and higher buffer capacity compared to conventional buffer-free fluids such as 0.9 % NaCl. This randomized clinical trial aimed to compare the effects of three infusion solutions - an acetate-containing balanced electrolyte solution (Sterofundin® ISO; Stero), 0.9 % saline (NaCl), and an isotonic saline-sodium bicarbonate mixture (NaClBic) - on acid-base status and plasma electrolyte concentrations in neonatal calves with diarrhoea. Thirty-three calves with diarrhoea, metabolic acidosis (plasma HCO3– < 25 mmol/L), and signs of clinical dehydration were enrolled. After initial correction of metabolic acidosis using 5.6 % sodium bicarbonate over 55 min, each of 11 calves randomly received one of the three infusion solutions at 8 mL/kg/h for 22 h. All three crystalloids had an acidifying effect, which was most pronounced in the NaCl group and least in the NaClBic group. At 18 and 24 h after initiation of treatment, plasma bicarbonate concentrations were significantly lower (P < 0.05) in the Stero and NaCl groups compared to the NaClBic group. Hyperchloraemia was observed in calves treated with 0.9 % NaCl. No statistically significant differences in clinical variables or plasma potassium, magnesium, and calcium concentrations were observed between groups after 24 h. In conclusion, the acetate-containing balanced solution did not provide a clinically relevant advantage over NaCl and NaClBic. However, findings support the use of chloride-restricted solutions for rehydration purposes in dehydrated neonatal calves with diarrhoea.
{"title":"Effects of intravenous rehydration protocols based on an acetate-containing electrolyte solution, isotonic saline, and an isotonic mixture of saline/sodium bicarbonate on acid-base status and plasma electrolyte concentrations in calves with diarrhoea","authors":"Dana Badura , Annette Lorch , Andrea Urgibl-Bauer , Antonia Kreuzer , Ingrid Lorenz , Yury Zablotski , Florian M. Trefz","doi":"10.1016/j.tvjl.2026.106571","DOIUrl":"10.1016/j.tvjl.2026.106571","url":null,"abstract":"<div><div>Strong ion (metabolic) acidosis, dehydration, and electrolyte imbalances are common in neonatal calves with diarrhoea. Balanced crystalloids may provide advantages for rehydration purposes in those animals due to their polyionic composition and higher buffer capacity compared to conventional buffer-free fluids such as 0.9 % NaCl. This randomized clinical trial aimed to compare the effects of three infusion solutions - an acetate-containing balanced electrolyte solution (Sterofundin® ISO; Stero), 0.9 % saline (NaCl), and an isotonic saline-sodium bicarbonate mixture (NaClBic) - on acid-base status and plasma electrolyte concentrations in neonatal calves with diarrhoea. Thirty-three calves with diarrhoea, metabolic acidosis (plasma HCO<sub>3</sub><sup>–</sup> < 25 mmol/L), and signs of clinical dehydration were enrolled. After initial correction of metabolic acidosis using 5.6 % sodium bicarbonate over 55 min, each of 11 calves randomly received one of the three infusion solutions at 8 mL/kg/h for 22 h. All three crystalloids had an acidifying effect, which was most pronounced in the NaCl group and least in the NaClBic group. At 18 and 24 h after initiation of treatment, plasma bicarbonate concentrations were significantly lower (<em>P</em> < 0.05) in the Stero and NaCl groups compared to the NaClBic group. Hyperchloraemia was observed in calves treated with 0.9 % NaCl. No statistically significant differences in clinical variables or plasma potassium, magnesium, and calcium concentrations were observed between groups after 24 h. In conclusion, the acetate-containing balanced solution did not provide a clinically relevant advantage over NaCl and NaClBic. However, findings support the use of chloride-restricted solutions for rehydration purposes in dehydrated neonatal calves with diarrhoea.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106571"},"PeriodicalIF":3.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.tvjl.2026.106569
Belén Larrán , Marta López-Alonso , Marta Miranda , Carlos Herrero-Latorre , María Luisa Suárez , Inmaculada Orjales
Trace elements are essential for physiological processes and have also been associated with tumour development, progression and prognosis in human medicine. This study aimed to characterize the plasma mineral profile of dogs with tumours and explore associations between elements and clinical findings. A total of 164 dogs with tumours (mammary, mast cell tumour, soft tissue sarcoma, intracranial, lymphoma, and hepatic tumour) and 40 controls were included. Plasma samples were analysed for As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Se, and Zn by mass spectrometry. Clinical findings and biochemical alterations were recorded. Compared to controls, dogs with tumours had higher concentrations of Cu (P < 0.001), Mn (P < 0.01), Ni (P < 0.001), Pb (P < 0.05), Se (P < 0.001), and Zn (P < 0.001). Element profiles differed by tumour type, with changes being particularly evident for hepatic tumours. Higher Cu and Mn concentrations and lower Se levels were associated with the presence of diverse clinical symptoms and distant metastasis. The study findings revealed different plasma trace element profiles in dogs with tumours and in controls, with variations linked to tumour type and clinical features. The findings suggest trace elements could be used as biomarkers for clinical staging or prognosis in canine tumours. Based on their informativeness in this study, Cu, Mn, Ni, Se, and Zn are recommended for inclusion in a core mineral panel for future research.
{"title":"Trace element profiles in canine neoplastic disease","authors":"Belén Larrán , Marta López-Alonso , Marta Miranda , Carlos Herrero-Latorre , María Luisa Suárez , Inmaculada Orjales","doi":"10.1016/j.tvjl.2026.106569","DOIUrl":"10.1016/j.tvjl.2026.106569","url":null,"abstract":"<div><div>Trace elements are essential for physiological processes and have also been associated with tumour development, progression and prognosis in human medicine. This study aimed to characterize the plasma mineral profile of dogs with tumours and explore associations between elements and clinical findings. A total of 164 dogs with tumours (mammary, mast cell tumour, soft tissue sarcoma, intracranial, lymphoma, and hepatic tumour) and 40 controls were included. Plasma samples were analysed for As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Se, and Zn by mass spectrometry. Clinical findings and biochemical alterations were recorded. Compared to controls, dogs with tumours had higher concentrations of Cu (P < 0.001), Mn (P < 0.01), Ni (P < 0.001), Pb (P < 0.05), Se (P < 0.001), and Zn (P < 0.001). Element profiles differed by tumour type, with changes being particularly evident for hepatic tumours. Higher Cu and Mn concentrations and lower Se levels were associated with the presence of diverse clinical symptoms and distant metastasis. The study findings revealed different plasma trace element profiles in dogs with tumours and in controls, with variations linked to tumour type and clinical features. The findings suggest trace elements could be used as biomarkers for clinical staging or prognosis in canine tumours. Based on their informativeness in this study, Cu, Mn, Ni, Se, and Zn are recommended for inclusion in a core mineral panel for future research.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106569"},"PeriodicalIF":3.1,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-17DOI: 10.1016/j.tvjl.2026.106568
Ingeborg Evensen , Cecilie Ersdal , Åse Margrethe Sogstad , Adam Dunstan Martin
According to on-farm emergency slaughter (OFES) veterinary certificates almost half of OFES cases in Norway are the result of locomotory disorders. However, beyond certification the diagnosis of these cases is unconfirmed. The aim of this study was to describe the gross lesions found at PME of cattle that underwent OFES for disorders of locomotion. During 2023, OFES cases entering two slaughterhouses underwent a regular PME and the pathological findings recorded. Complementary data were obtained from the veterinary certificate and Norwegian Food Safety Authority’s record system. In total, 492 cases met the study’s inclusion criteria. Descriptive statistics were generated, and logistic regression analyses were performed to examine relationship of sex, breed, production form and age with the post-mortem diagnosis. Gross lesions found at PME were categorized. The most common categories were soft tissue trauma (24 %), fracture (23 %), joint pathology (23 %), and joint dislocation (18 %). Most joint pathology cases involved the hock joint with males having a higher risk than females. Multivariable analysis found that joint dislocations (93 % hip dislocations) were associated with older, female Holstein cows. The high number of trauma cases was to be expected as suffering an accident is a prerequisite for the animal being eligible for OFES. However, the high number of spinal fractures, hip dislocations in female cattle, as well as hock lesions in bulls should be investigated further to find ways to prevent these painful lesions.
{"title":"Postmortem findings in lame cattle which underwent on farm emergency slaughter in Norway","authors":"Ingeborg Evensen , Cecilie Ersdal , Åse Margrethe Sogstad , Adam Dunstan Martin","doi":"10.1016/j.tvjl.2026.106568","DOIUrl":"10.1016/j.tvjl.2026.106568","url":null,"abstract":"<div><div>According to on-farm emergency slaughter (OFES) veterinary certificates almost half of OFES cases in Norway are the result of locomotory disorders. However, beyond certification the diagnosis of these cases is unconfirmed. The aim of this study was to describe the gross lesions found at PME of cattle that underwent OFES for disorders of locomotion. During 2023, OFES cases entering two slaughterhouses underwent a regular PME and the pathological findings recorded. Complementary data were obtained from the veterinary certificate and Norwegian Food Safety Authority’s record system. In total, 492 cases met the study’s inclusion criteria. Descriptive statistics were generated, and logistic regression analyses were performed to examine relationship of sex, breed, production form and age with the post-mortem diagnosis. Gross lesions found at PME were categorized. The most common categories were soft tissue trauma (24 %), fracture (23 %), joint pathology (23 %), and joint dislocation (18 %). Most joint pathology cases involved the hock joint with males having a higher risk than females. Multivariable analysis found that joint dislocations (93 % hip dislocations) were associated with older, female Holstein cows. The high number of trauma cases was to be expected as suffering an accident is a prerequisite for the animal being eligible for OFES. However, the high number of spinal fractures, hip dislocations in female cattle, as well as hock lesions in bulls should be investigated further to find ways to prevent these painful lesions.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106568"},"PeriodicalIF":3.1,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146004243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-17DOI: 10.1016/j.tvjl.2026.106560
Barbara Błaszczyk , Tomasz Stankiewicz , Małgorzata A. Szewczuk , Jan Udała , Arkadiusz Pietruszka
The aim of the study was to determine Doppler parameters in the common carotid arteries, temporal arteries and vertebral arteries of Suffolk lambs in the first month of life. Peak systolic velocity (PSV), end diastolic velocity (EDV), PSV/EDV ratio, resistance index (RI) and pulsatility index (PI) were measured in the examined arteries. The relationships between the examined parameters, the age and sex of the lambs, as well as the location of the arterial vessel, were determined. It was observed that the values of Doppler parameters depended on both the age of lambs and the location of the artery (P < 0.01). It was also shown that the Doppler parameters depended on both the gender and the location of the artery (P < 0.05). Most Doppler parameters measured in the carotid arteries were significantly different than those recorded in the temporal arteries (P < 0.01) and the vertebral arteries (P < 0.01). In the examined arteries, PSV, PSV/EDV and RI were significantly higher in ram lambs than in ewe lambs (P < 0.01 and P < 0.05). The study underlines the importance of taking into account the gender, location of the vessels and the postnatal age of the lambs when interpreting results.
{"title":"Doppler parameters in the common carotid, temporal and vertebral arteries in Suffolk lambs in the first month of life","authors":"Barbara Błaszczyk , Tomasz Stankiewicz , Małgorzata A. Szewczuk , Jan Udała , Arkadiusz Pietruszka","doi":"10.1016/j.tvjl.2026.106560","DOIUrl":"10.1016/j.tvjl.2026.106560","url":null,"abstract":"<div><div>The aim of the study was to determine Doppler parameters in the common carotid arteries, temporal arteries and vertebral arteries of Suffolk lambs in the first month of life. Peak systolic velocity (PSV), end diastolic velocity (EDV), PSV/EDV ratio, resistance index (RI) and pulsatility index (PI) were measured in the examined arteries. The relationships between the examined parameters, the age and sex of the lambs, as well as the location of the arterial vessel, were determined. It was observed that the values of Doppler parameters depended on both the age of lambs and the location of the artery (P < 0.01). It was also shown that the Doppler parameters depended on both the gender and the location of the artery (P < 0.05). Most Doppler parameters measured in the carotid arteries were significantly different than those recorded in the temporal arteries (P < 0.01) and the vertebral arteries (P < 0.01). In the examined arteries, PSV, PSV/EDV and RI were significantly higher in ram lambs than in ewe lambs (P < 0.01 and P < 0.05). The study underlines the importance of taking into account the gender, location of the vessels and the postnatal age of the lambs when interpreting results.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106560"},"PeriodicalIF":3.1,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146004266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-16DOI: 10.1016/j.tvjl.2026.106557
Imadidden Musallam , Julio Pinto , Ravi Bandara Dissanayake , Muhammad Usman Zaheer , Zaidoun Hijazeen , Wafa’a Ramadneh , Iqbal Qatananni , Esam Hawa , Majed Hawaowsheh , Ehab Abu-Basha , Aida binti Muhid , Jamaliah binti Senawi , Mariani binti Hashim , Muhammad Nazri bin Khairuddin , Janice Garcia , Blesilda C. Verin , Jasmine Magtibay , Anil Demeli , Kemal Yilmaz , Sedat Ildiz , Javier Guitian
A training needs assessment (TNA) toolkit was developed by adapting the Hennessy–Hicks questionnaire to evaluate field veterinary epidemiology training needs within the national Veterinary Services of Jordan, Malaysia, the Philippines, Turkey, and Ukraine. The toolkit comprises two questionnaires that assess frontline and intermediate field epidemiology core competencies defined by the FAO and can be used to evaluate training needs at both individual and organisational levels.
Frontline veterinarians in the five countries completed the questionnaires electronically. Additionally, face-to-face workshops held in Jordan, Malaysia, the Philippines, and Turkey—attended by key informants, line managers, and frontline veterinarians—provided opportunities for deeper discussion and refinement of training needs.
Training Needs Indexes (TNIs), defined as the difference between criticality and current performance levels and derived from responses of frontline veterinarians and their supervisors, indicated that most of the 32 frontline and 30 intermediate competencies were considered critical for effective performance. However, current performance ratings varied across countries, with higher scores in Jordan, Turkey, and Ukraine, and lower scores in Malaysia and the Philippines. TNIs from group discussions were higher than those from individual online responses, suggesting that veterinarians may overestimate their performance when self-assessing.
Differences in frequency ratings reflected the diverse roles of frontline veterinarians across countries, shaping which competencies were prioritised for further training. The toolkit’s reliance on self-assessment was identified as a limitation, though this can be mitigated by supplementing individual assessments with group discussions. Competencies with high TNIs at both levels were classified as priorities to guide future training activities.
{"title":"A toolkit for the assessment of training needs and gaps of the national Veterinary Services in field epidemiology","authors":"Imadidden Musallam , Julio Pinto , Ravi Bandara Dissanayake , Muhammad Usman Zaheer , Zaidoun Hijazeen , Wafa’a Ramadneh , Iqbal Qatananni , Esam Hawa , Majed Hawaowsheh , Ehab Abu-Basha , Aida binti Muhid , Jamaliah binti Senawi , Mariani binti Hashim , Muhammad Nazri bin Khairuddin , Janice Garcia , Blesilda C. Verin , Jasmine Magtibay , Anil Demeli , Kemal Yilmaz , Sedat Ildiz , Javier Guitian","doi":"10.1016/j.tvjl.2026.106557","DOIUrl":"10.1016/j.tvjl.2026.106557","url":null,"abstract":"<div><div>A training needs assessment (TNA) toolkit was developed by adapting the Hennessy–Hicks questionnaire to evaluate field veterinary epidemiology training needs within the national Veterinary Services of Jordan, Malaysia, the Philippines, Turkey, and Ukraine. The toolkit comprises two questionnaires that assess frontline and intermediate field epidemiology core competencies defined by the FAO and can be used to evaluate training needs at both individual and organisational levels.</div><div>Frontline veterinarians in the five countries completed the questionnaires electronically. Additionally, face-to-face workshops held in Jordan, Malaysia, the Philippines, and Turkey—attended by key informants, line managers, and frontline veterinarians—provided opportunities for deeper discussion and refinement of training needs.</div><div>Training Needs Indexes (TNIs), defined as the difference between criticality and current performance levels and derived from responses of frontline veterinarians and their supervisors, indicated that most of the 32 frontline and 30 intermediate competencies were considered critical for effective performance. However, current performance ratings varied across countries, with higher scores in Jordan, Turkey, and Ukraine, and lower scores in Malaysia and the Philippines. TNIs from group discussions were higher than those from individual online responses, suggesting that veterinarians may overestimate their performance when self-assessing.</div><div>Differences in frequency ratings reflected the diverse roles of frontline veterinarians across countries, shaping which competencies were prioritised for further training. The toolkit’s reliance on self-assessment was identified as a limitation, though this can be mitigated by supplementing individual assessments with group discussions. Competencies with high TNIs at both levels were classified as priorities to guide future training activities.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106557"},"PeriodicalIF":3.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-15DOI: 10.1016/j.tvjl.2026.106564
Íris J. Fidalgo , Joana C. Prata
Plant poisoning in animals, often associated with wild and ornamental species, is a significant concern in veterinary medicine. Due to clinical signs observed in humans after consuming “mad honey”, interest in grayanotoxins, produced by Ericaceae plants, has increased. However, effects on animal health have been scarcely addressed despite their interest for veterinary toxicology. This systematic review, following PRISMA guidelines, identified 31 records of grayanotoxin poisoning in animals following the ingestion of plants, affecting a total of 111 livestock and 11 companion animals. Cases were predominantly reported in livestock, namely sheep and goats, and less frequently in companion animals, including dogs, cats, rabbits, tortoises, and pigs. Studies report an estimated toxic dose of 0.1 % of body weight in fresh foliage for ruminants. Rhododendron spp. and Pieris japonica were the most frequently reported plants. Common clinical signs and treatment were summarized. Retching, regurgitation, and vomiting were common and might be suggestive of plant poisoning in ruminants. Reports on grayanotoxin poisoning in animals must be improved to address existing knowledge gaps.
{"title":"Grayanotoxin poisoning in animals following the ingestion of Ericaceae plants","authors":"Íris J. Fidalgo , Joana C. Prata","doi":"10.1016/j.tvjl.2026.106564","DOIUrl":"10.1016/j.tvjl.2026.106564","url":null,"abstract":"<div><div>Plant poisoning in animals, often associated with wild and ornamental species, is a significant concern in veterinary medicine. Due to clinical signs observed in humans after consuming “mad honey”, interest in grayanotoxins, produced by Ericaceae plants, has increased. However, effects on animal health have been scarcely addressed despite their interest for veterinary toxicology. This systematic review, following PRISMA guidelines, identified 31 records of grayanotoxin poisoning in animals following the ingestion of plants, affecting a total of 111 livestock and 11 companion animals. Cases were predominantly reported in livestock, namely sheep and goats, and less frequently in companion animals, including dogs, cats, rabbits, tortoises, and pigs. Studies report an estimated toxic dose of 0.1 % of body weight in fresh foliage for ruminants. <em>Rhododendron</em> spp. and <em>Pieris japonica</em> were the most frequently reported plants. Common clinical signs and treatment were summarized. Retching, regurgitation, and vomiting were common and might be suggestive of plant poisoning in ruminants. Reports on grayanotoxin poisoning in animals must be improved to address existing knowledge gaps.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"315 ","pages":"Article 106564"},"PeriodicalIF":3.1,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}