V E Nebol'sin, V V Krzhechkovskaia, G A Zheltukhina, R P Evstigneeva, V L Kovaleva
gamma-L-Glutamyl-histamine (gamma-GluHA) was synthesized at Department of the chemistry and technology of fine organic compounds M. V. Lomonosov Moscow state academy of fine chemical technology. It was investigated the influence of gamma-L-glutamyl-histamine on the severity of experimental anaphylactic reaction, hormonal content in blood plasma and liver cytochrome P-450. The induction of liver cytochrome P-450, the elevation of hormonal content (thyroxine and glucocorticoids) in blood plasma and decrease of the severity of experimental anaphylactic reaction are observed.
{"title":"[The effect of gamma-L-glutamyl histamine on the severity of experimental anaphylactic reaction, hormonal status and liver cytochrome p-450].","authors":"V E Nebol'sin, V V Krzhechkovskaia, G A Zheltukhina, R P Evstigneeva, V L Kovaleva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>gamma-L-Glutamyl-histamine (gamma-GluHA) was synthesized at Department of the chemistry and technology of fine organic compounds M. V. Lomonosov Moscow state academy of fine chemical technology. It was investigated the influence of gamma-L-glutamyl-histamine on the severity of experimental anaphylactic reaction, hormonal content in blood plasma and liver cytochrome P-450. The induction of liver cytochrome P-450, the elevation of hormonal content (thyroxine and glucocorticoids) in blood plasma and decrease of the severity of experimental anaphylactic reaction are observed.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"482-8"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L F Panchenko, S V Pirozhkov, A V Nadezhdin, V Iu Baronets, N N Usmanova
Chronic opiate intoxication has been shown to cause various pathologic changes in the liver almost in 100% of cases. Earlier it has been demonstrated that acute or chronic morphine intoxication evokes activation of lipid peroxidation in the liver, heart, and brain cells. The aim of the present work was to assess parameters reflecting cytolysis in the liver and heart, and the plasma content of factors contributing to the peroxyl radical-scavenging system of the blood of teenagers using heroin. Blood samples were obtained from 20 male patients from 14 to 16 years old, with a mean duration of regular heroin use of 1.7 years. The control group included 13 healthy teenagers which denied the previous drug use. Mean plasma ALT and myocardial isoform of LDH activities were significantly higher (1.7- and 1.4-times respectively) in the heroin users than in the control group. The mean plasma level of lipid peroxides in the heroin users is increased by 20% compared to the control individuals. In teenagers using heroin a high level of correlation was observed between the plasma content of lipid peroxides and myocardial LDH activity (r = 0.76; P < 0.01). The effect of heroin use on the content of the plasma peroxyl radical-scavenging factors--vitamin E, ascorbic acid, and protein SH-groups--was not found. It has been concluded that heart injury during heroin use in teenagers may be associated with activation of lipid peroxidation reactions in the myocardium.
{"title":"[Lipid peroxidation, peroxyl radical-scavenging system of plasma and liver and heart pathology in adolescence heroin users].","authors":"L F Panchenko, S V Pirozhkov, A V Nadezhdin, V Iu Baronets, N N Usmanova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chronic opiate intoxication has been shown to cause various pathologic changes in the liver almost in 100% of cases. Earlier it has been demonstrated that acute or chronic morphine intoxication evokes activation of lipid peroxidation in the liver, heart, and brain cells. The aim of the present work was to assess parameters reflecting cytolysis in the liver and heart, and the plasma content of factors contributing to the peroxyl radical-scavenging system of the blood of teenagers using heroin. Blood samples were obtained from 20 male patients from 14 to 16 years old, with a mean duration of regular heroin use of 1.7 years. The control group included 13 healthy teenagers which denied the previous drug use. Mean plasma ALT and myocardial isoform of LDH activities were significantly higher (1.7- and 1.4-times respectively) in the heroin users than in the control group. The mean plasma level of lipid peroxides in the heroin users is increased by 20% compared to the control individuals. In teenagers using heroin a high level of correlation was observed between the plasma content of lipid peroxides and myocardial LDH activity (r = 0.76; P < 0.01). The effect of heroin use on the content of the plasma peroxyl radical-scavenging factors--vitamin E, ascorbic acid, and protein SH-groups--was not found. It has been concluded that heart injury during heroin use in teenagers may be associated with activation of lipid peroxidation reactions in the myocardium.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"501-6"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A I Luĭk, V V Prokopenko, V Iu Tanchuk, V V Kholodovich, I V Semeniuta
Using experimental transcription model in vitro, and method of electron-topological calculations the elements of pharmacological and structural community have been found in the groups of physiologically active compounds (PAC), blocking and activating external cellular receptors. This finding confirms the necessity of subdivision special groups of bio-substrates blockators and activators for hierarchical classification, of PAC.
{"title":"[Common properties of pharmacological agonists and antagonists of surface membrane receptors].","authors":"A I Luĭk, V V Prokopenko, V Iu Tanchuk, V V Kholodovich, I V Semeniuta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using experimental transcription model in vitro, and method of electron-topological calculations the elements of pharmacological and structural community have been found in the groups of physiologically active compounds (PAC), blocking and activating external cellular receptors. This finding confirms the necessity of subdivision special groups of bio-substrates blockators and activators for hierarchical classification, of PAC.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"514-24"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Three basic functions of vitamin K in organism are considered. Besides (vitamin K-dependent) posttranslational modification of Ca-binding proteins, this vitamin can operate via vitamin D hormonal system, and can directly influence membranes.
{"title":"[Functional role of vitamin K].","authors":"A A Sokol'nikov, V M Kodentsova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three basic functions of vitamin K in organism are considered. Besides (vitamin K-dependent) posttranslational modification of Ca-binding proteins, this vitamin can operate via vitamin D hormonal system, and can directly influence membranes.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"453-61"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V B Prozorovskiĭ, L V Pavlova, I M Suslova, A V Sazonova, A V Kokushkina
A series of aminostigmin derivatives with various substituents at nitrogen in the second position of the pyridine ring, has been tested. The efficacy of preventing the death of mice poisoned by armine in five of the seven substances correlates with the constant of the rate of carbamylation of acetylcholinesterase in the in vitro experiments and with the hydrophobic nature. It is suggested that the phenomenon of protection of animals against the toxic effect of organophosphorous compounds involves the "leaving portion" of the molecule of carbamates.
{"title":"[Protection of mice by carbamates cholinesterase inhibitors against poisoning by armin and its dependence on certain physico-chemical indicators].","authors":"V B Prozorovskiĭ, L V Pavlova, I M Suslova, A V Sazonova, A V Kokushkina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A series of aminostigmin derivatives with various substituents at nitrogen in the second position of the pyridine ring, has been tested. The efficacy of preventing the death of mice poisoned by armine in five of the seven substances correlates with the constant of the rate of carbamylation of acetylcholinesterase in the in vitro experiments and with the hydrophobic nature. It is suggested that the phenomenon of protection of animals against the toxic effect of organophosphorous compounds involves the \"leaving portion\" of the molecule of carbamates.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"525-9"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A V Alesenko, E S Zubova, L B Dudnik, E I Gal'perin, L V Platonova, N Shono, A Iu Chevokin, E V Diatlovitskaia
Activity of neutral and acidic sphingomyelinases (N- and A-SMases) were studied in regenerating liver after partial hepatectomy (during 48 hrs after operation), in ischemic liver during 15, 30 min and 1 and 2 hrs ischemia and during following reperfusion (from 5 min up to 2 hrs), in hepatoma- 22 after 15 days of transplantation and in liver of tumor bearing animals. It was shown that activity of N-SMase is increased in hepatoma-22 and in regenerating liver and it is decreased in ischemic liver. Following reperfusion of ischemic liver area activity of enzyme was found to have returned to baseline in dependence on time of ischemia and reperfusion. Activity of A-SMase is decreased in tumor, is not changed in regenerating liver and increased after long time of ischemia. It was supposed that N-SMase is involved in cell proliferation, but A-SMase is connected with cell damage.
{"title":"[Changes in the activity of neutral and acidic isoforms of sphingomyelinase in hepatoma-22, regenerating and ischemic liver].","authors":"A V Alesenko, E S Zubova, L B Dudnik, E I Gal'perin, L V Platonova, N Shono, A Iu Chevokin, E V Diatlovitskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Activity of neutral and acidic sphingomyelinases (N- and A-SMases) were studied in regenerating liver after partial hepatectomy (during 48 hrs after operation), in ischemic liver during 15, 30 min and 1 and 2 hrs ischemia and during following reperfusion (from 5 min up to 2 hrs), in hepatoma- 22 after 15 days of transplantation and in liver of tumor bearing animals. It was shown that activity of N-SMase is increased in hepatoma-22 and in regenerating liver and it is decreased in ischemic liver. Following reperfusion of ischemic liver area activity of enzyme was found to have returned to baseline in dependence on time of ischemia and reperfusion. Activity of A-SMase is decreased in tumor, is not changed in regenerating liver and increased after long time of ischemia. It was supposed that N-SMase is involved in cell proliferation, but A-SMase is connected with cell damage.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"472-81"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Iu Abramova, Iu A Borisov, A V Bratov, P V Gavrilenkov, K Dominguez, V N Spiridonov, E D Suglobova
The applicability is examined of ion selective field transistors with photocurable polyurethane membranes to control of the electrolytic composition of dialyzing solutions used in artificial kidney apparatus, and also of plasma in patient treated by chronic hemodialysis. The short- and long-time characteristics of the efficiency of K(+)-selective field transistors in continuous contact with solutions. Comparative testing of such transistors and other sensing systems is made. It is demonstrated that a sufficient reliability of measurements in the range of physiological concentrations in combination with low cost provide the possibility of using K(+)-selective field transistors for monitoring of the potassium concentration both in dialyzing solutions and plasma in patients on chronic hemodialysis treatment.
{"title":"[The use of ion-selective field transistors (ISFT) with photopolymerizable polyurethane membranes in nephrology for determination of potassium concentration (activity)].","authors":"N Iu Abramova, Iu A Borisov, A V Bratov, P V Gavrilenkov, K Dominguez, V N Spiridonov, E D Suglobova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The applicability is examined of ion selective field transistors with photocurable polyurethane membranes to control of the electrolytic composition of dialyzing solutions used in artificial kidney apparatus, and also of plasma in patient treated by chronic hemodialysis. The short- and long-time characteristics of the efficiency of K(+)-selective field transistors in continuous contact with solutions. Comparative testing of such transistors and other sensing systems is made. It is demonstrated that a sufficient reliability of measurements in the range of physiological concentrations in combination with low cost provide the possibility of using K(+)-selective field transistors for monitoring of the potassium concentration both in dialyzing solutions and plasma in patients on chronic hemodialysis treatment.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"530-8"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V V Lebedev, P P Golikov, B V Davydov, K I Rakhimi, A G Suvalian, N Iu Nikolaeva, N V Fedorova, V V Marchenko
In 31 patients after a craniocerebral trauma combined with a trauma of locomotor system (CCCT), and in 28 patients with only a limb fractures a time course (1, 3, 7, and 14 days) of biochemical stress reaction inductors (cortisol, serotonin, histamine, lipid peroxidation products) in various tactics of surgical treatment was studied. It was established that CCCT in a significantly stronger degree increases cortisol and lipid peroxidation products' levels than an isolated locomotor system trauma. An early postponed surgical intervention in lower extremities in CCCT results in the same increase in the biochemical stress reaction inductors as a late postpone surgical intervention in the lower extremities. In an early postponed osteosynthesis in CCCT patients, a mean term of hospital stay was 67 days, and in a late postponed one 117 days. The disability period was correspondingly 200 vs 315 days.
{"title":"[The dynamic of biochemical stress reaction inductors in various tactics for surgical treatment of patients with a combined trauma of cranium and lower limbs ].","authors":"V V Lebedev, P P Golikov, B V Davydov, K I Rakhimi, A G Suvalian, N Iu Nikolaeva, N V Fedorova, V V Marchenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In 31 patients after a craniocerebral trauma combined with a trauma of locomotor system (CCCT), and in 28 patients with only a limb fractures a time course (1, 3, 7, and 14 days) of biochemical stress reaction inductors (cortisol, serotonin, histamine, lipid peroxidation products) in various tactics of surgical treatment was studied. It was established that CCCT in a significantly stronger degree increases cortisol and lipid peroxidation products' levels than an isolated locomotor system trauma. An early postponed surgical intervention in lower extremities in CCCT results in the same increase in the biochemical stress reaction inductors as a late postpone surgical intervention in the lower extremities. In an early postponed osteosynthesis in CCCT patients, a mean term of hospital stay was 67 days, and in a late postponed one 117 days. The disability period was correspondingly 200 vs 315 days.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"494-500"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A I Lukash, V G Zaika, N P Miliutina, A O Kucherenko
The intensity of free radical processes (FRP) and the activity of antioxidant (AO) systems in human blood plasma and saliva were studied under physiological emotional strain and depressive pathologies (before and after treatment). The significant alterations in the dynamics of investigative indexes under different emotional strain were revealed. These alterations in saliva were more intensive than in blood plasma. The treatment led to the stabilization of FRP intensity and the activity of AO protection. The correlation between saliva and blood plasma parametres allows to use saliva for the estimation of emotional strain and of the efficiency of therapeutics under depressive pathologies.
{"title":"[Intensity of free radical process and antioxidant enzymes activity in human saliva and plasma under emotional stress].","authors":"A I Lukash, V G Zaika, N P Miliutina, A O Kucherenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intensity of free radical processes (FRP) and the activity of antioxidant (AO) systems in human blood plasma and saliva were studied under physiological emotional strain and depressive pathologies (before and after treatment). The significant alterations in the dynamics of investigative indexes under different emotional strain were revealed. These alterations in saliva were more intensive than in blood plasma. The treatment led to the stabilization of FRP intensity and the activity of AO protection. The correlation between saliva and blood plasma parametres allows to use saliva for the estimation of emotional strain and of the efficiency of therapeutics under depressive pathologies.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"507-13"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E M Krupitskiĭ, G F Karandashova, V V Vostrikov, N M Lavrova, N I Grinenko, T Iu Slavina, A Ia Grinenko
The influence of 400 mM ethanol on the activity of monoamine oxidase type B (MAO-B) of blood platelets has been studied in vitro in 30 alcoholic patients and 30 healthy volunteers. Benzylamine was used as a substrate for MAO-B. MAO-B inhibition by ethanol was higher in alcoholics compared to healthy volunteers. The higher vulnerability of MAO-B of alcoholics inhibiting action to of ethanol may be one of the mechanisms underlying some symptoms of alcoholism.
{"title":"[Ethanol inhibits platelet monoamine oxidase type B activity in patients with alcoholism].","authors":"E M Krupitskiĭ, G F Karandashova, V V Vostrikov, N M Lavrova, N I Grinenko, T Iu Slavina, A Ia Grinenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of 400 mM ethanol on the activity of monoamine oxidase type B (MAO-B) of blood platelets has been studied in vitro in 30 alcoholic patients and 30 healthy volunteers. Benzylamine was used as a substrate for MAO-B. MAO-B inhibition by ethanol was higher in alcoholics compared to healthy volunteers. The higher vulnerability of MAO-B of alcoholics inhibiting action to of ethanol may be one of the mechanisms underlying some symptoms of alcoholism.</p>","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"45 6","pages":"489-93"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21611108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}