Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.42
A.G. Starovoitov, A. Lipnitski, A. Marochkov
Currently, the most common method in clinical practice, that enables the determination of the functional state of platelets, is the method of platelet aggregation. Objectives. To study the aggregation of platelets in donors of platelet mass and fresh frozen plasma. Material and methods. The study included 31 donors of fresh frozen plasma and platelet mass. The study of platelet aggregation was carried out by the turbidimetric measurement technique on the AP2110 analyzer (ZAO «SOLAR», Minsk, Republic of Belarus) with an inductor: ADP (concentrations of 0.3 μg/ml, 0.6 μg/ml, 1.25 μg/ml and 2.5 μg/ml were used), epinephrine (concentrations of 2.5 μM and 5.0 μM were used), collagen (concentration of 2 mg/ml). Results. 33 analysis of platelet aggregation were performed in donors prior to collection of platelet mass and fresh frozen plasma. Only in 10 cases (30.3%) donors had normal platelet aggregation, hypoaggregation was detected in 15 cases (45.5%) and hyperaggregation - in 8 studies (24.2%). Impaired platelet aggregation was detected in all donors who had COVID-19 infection in their anamnesis (in 5 - hypoaggregation, in 4 - hyperaggregation). In donors who didn’t have this infection in their anamnesis, the incidence of platelet aggregation disorders was statistically significantly lower - 63.6% (in 10 - hypoaggregation, in 4 - hyperaggregation), p=0.04. The presence of COVID-19 infection in the donor’s anamnesis is an independent predictor of impaired platelet aggregation (Chi-square=7.24, p=0.007). Conclusions. The study of platelet aggregation must be mandatory before collecting platelet mass in all donors.
{"title":"PLATELET AGGREGATION IN DONORS OF PLATELET MASS AND FRESH FROZEN PLASMA","authors":"A.G. Starovoitov, A. Lipnitski, A. Marochkov","doi":"10.22263/2312-4156.2021.5.42","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.42","url":null,"abstract":"Currently, the most common method in clinical practice, that enables the determination of the functional state of platelets, is the method of platelet aggregation. Objectives. To study the aggregation of platelets in donors of platelet mass and fresh frozen plasma. Material and methods. The study included 31 donors of fresh frozen plasma and platelet mass. The study of platelet aggregation was carried out by the turbidimetric measurement technique on the AP2110 analyzer (ZAO «SOLAR», Minsk, Republic of Belarus) with an inductor: ADP (concentrations of 0.3 μg/ml, 0.6 μg/ml, 1.25 μg/ml and 2.5 μg/ml were used), epinephrine (concentrations of 2.5 μM and 5.0 μM were used), collagen (concentration of 2 mg/ml). Results. 33 analysis of platelet aggregation were performed in donors prior to collection of platelet mass and fresh frozen plasma. Only in 10 cases (30.3%) donors had normal platelet aggregation, hypoaggregation was detected in 15 cases (45.5%) and hyperaggregation - in 8 studies (24.2%). Impaired platelet aggregation was detected in all donors who had COVID-19 infection in their anamnesis (in 5 - hypoaggregation, in 4 - hyperaggregation). In donors who didn’t have this infection in their anamnesis, the incidence of platelet aggregation disorders was statistically significantly lower - 63.6% (in 10 - hypoaggregation, in 4 - hyperaggregation), p=0.04. The presence of COVID-19 infection in the donor’s anamnesis is an independent predictor of impaired platelet aggregation (Chi-square=7.24, p=0.007). Conclusions. The study of platelet aggregation must be mandatory before collecting platelet mass in all donors.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86990006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.34
T. A. Petrovskaya, E. Karpova, D. Tapalski, L. Mozharovskaya, O. Baranov
Identification of numerous mechanisms of resistance to colistin and other antibiotics is possible using whole genome sequencing. Objectives. To assess the molecular-genetic mechanisms of resistance to polymyxins and antibiotics of other groups in nosocomial Klebsiella pneumoniae strains. Material and methods. For 13 multidrug- and extensively drug-resistant K.pneumoniae strains semiconductor sequencing was performed in the Ion PGM System genomic sequencer (Thermo Fisher Scientific, USA). The assembly of genomic sequences and their annotation were carried out. The PROVEAN software tool was used to predict the influence of nucleotide replacements on the structure of amino acid sequences and functional activity of proteins. The identification of antibiotic resistance genes and the search for efflux mechanisms were performed by the ResFinder v.4.1 and CARD web resources. Results. Several types of β-lactamase genes were detected simultaneously in all strains, as well as genes of resistance to fosfomycin. Genes of resistance to aminoglycosides were identified in 11 strains, to chloramphenicol - in 10, to rifampicin - in 5, to macrolides - in 4. The mcr phosphoethanolamine transferase genes were absent in all strains. Functionally significant substitutions were revealed in the pmrB gene (D150Y, T157P, G207S) comparing the studied samples with the reference K. pneumoniae strain ATCC 700603. Changes in the mgrB gene were also found in colistin-resistant strains (W20R replacement, insertional inactivation of the gene by transposons of the IS1, IS4 and IS5 families). Conclusions. The results of whole genome sequencing represent the significant resistance of nosocomial Klebsiella pneumoniae strains to the majority of antibiotics including β-lactams, aminoglycosides, fluoroquinolones, fosfomycin, chloramphenicol, polymyxins. Genetic determinants of colistin resistance were revealed (insertional inactivation and deletion of the mgrB gene; D150Y, T157P and G207S substitutions in the pmrB gene) in strains with colistin MIC 64-128 mg/l and their absence in colistin-susceptible strains.
{"title":"MOLECULAR-GENETIC MECHANISMS OF RESISTANCE OF NOSOCOMIAL KLEBSIELLA PNEUMONIAE STRAINS TO POLYMYXINS AND ANTIBIOTICS OF OTHER GROUPS ACCORDING TO WHOLE GENOME SEQUENCING DATA","authors":"T. A. Petrovskaya, E. Karpova, D. Tapalski, L. Mozharovskaya, O. Baranov","doi":"10.22263/2312-4156.2021.5.34","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.34","url":null,"abstract":"Identification of numerous mechanisms of resistance to colistin and other antibiotics is possible using whole genome sequencing. Objectives. To assess the molecular-genetic mechanisms of resistance to polymyxins and antibiotics of other groups in nosocomial Klebsiella pneumoniae strains. Material and methods. For 13 multidrug- and extensively drug-resistant K.pneumoniae strains semiconductor sequencing was performed in the Ion PGM System genomic sequencer (Thermo Fisher Scientific, USA). The assembly of genomic sequences and their annotation were carried out. The PROVEAN software tool was used to predict the influence of nucleotide replacements on the structure of amino acid sequences and functional activity of proteins. The identification of antibiotic resistance genes and the search for efflux mechanisms were performed by the ResFinder v.4.1 and CARD web resources. Results. Several types of β-lactamase genes were detected simultaneously in all strains, as well as genes of resistance to fosfomycin. Genes of resistance to aminoglycosides were identified in 11 strains, to chloramphenicol - in 10, to rifampicin - in 5, to macrolides - in 4. The mcr phosphoethanolamine transferase genes were absent in all strains. Functionally significant substitutions were revealed in the pmrB gene (D150Y, T157P, G207S) comparing the studied samples with the reference K. pneumoniae strain ATCC 700603. Changes in the mgrB gene were also found in colistin-resistant strains (W20R replacement, insertional inactivation of the gene by transposons of the IS1, IS4 and IS5 families). Conclusions. The results of whole genome sequencing represent the significant resistance of nosocomial Klebsiella pneumoniae strains to the majority of antibiotics including β-lactams, aminoglycosides, fluoroquinolones, fosfomycin, chloramphenicol, polymyxins. Genetic determinants of colistin resistance were revealed (insertional inactivation and deletion of the mgrB gene; D150Y, T157P and G207S substitutions in the pmrB gene) in strains with colistin MIC 64-128 mg/l and their absence in colistin-susceptible strains.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84687432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.58
D. V. Turliuk, N. Rogovoy, S. Alexeev, V. Yanushko
Today V3 is the rarest and most difficult to access segment of the vertebral artery (VA). Material and methods. A sectional study was performed in 35 patients who died from various causes to study the topographic and anatomical characteristics of the VA anatomy in V3. The technique of access to the VA: along the inner edge of the right sternocleidomastoid muscle, a linear incision 6.2+1.5 cm long was performed, starting from the level of 2.1+0.8 cm above the angle of the lower jaw along the submandibular fold towards the «tragus» auricle. The common carotid artery, the internal carotid artery (ICA), the jugular vein, and the external carotid artery up to the third segment were isolated with excision and ligation of the lateral branches. The dissection of the salivary gland was not carried out, since it is quite mobile and is well retracted by the retractor hooks, as well as the venous plexus, nerves, «digastric» muscle. Results. The original approach proposed by us suggests reducing surgical trauma, as well as reducing the risk of developing damage to the neurovascular structures in the intervention zone. When working on the sectional material, the distance between the distal portion of the ICA and the third segment of the VA was determined. It did not exceed, according to our data, 3.2+2.1 mm. This fact allows, if necessary, to safely form an anastomosis between the ICA and the VA in the V3 segment according to the «side-to-side» type. Conclusions. The proposed access to the third segment of the VA enables the reduction of surgical trauma by reducing the incision length up to 6.2+1.2 cm, as well as by mobilizing anatomical structures in the surgical wound without crossing them (salivary gland, jugular vein, vagus nerve, C2 - a portion of the brachial plexus, digastric muscle, internal carotid artery). With concomitant tortuosity of the ICA, the excess part of the ICA is the optimal material for bypassing the VA in the third segment of the VA. A variant of surgical correction in this situation is the formation of «trifurcation» of the carotid artery.
{"title":"SURGICAL ACCESS TO THE THIRD SEGMENT OF THE VERTEBRAL ARTERY","authors":"D. V. Turliuk, N. Rogovoy, S. Alexeev, V. Yanushko","doi":"10.22263/2312-4156.2021.5.58","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.58","url":null,"abstract":"Today V3 is the rarest and most difficult to access segment of the vertebral artery (VA). Material and methods. A sectional study was performed in 35 patients who died from various causes to study the topographic and anatomical characteristics of the VA anatomy in V3. The technique of access to the VA: along the inner edge of the right sternocleidomastoid muscle, a linear incision 6.2+1.5 cm long was performed, starting from the level of 2.1+0.8 cm above the angle of the lower jaw along the submandibular fold towards the «tragus» auricle. The common carotid artery, the internal carotid artery (ICA), the jugular vein, and the external carotid artery up to the third segment were isolated with excision and ligation of the lateral branches. The dissection of the salivary gland was not carried out, since it is quite mobile and is well retracted by the retractor hooks, as well as the venous plexus, nerves, «digastric» muscle. Results. The original approach proposed by us suggests reducing surgical trauma, as well as reducing the risk of developing damage to the neurovascular structures in the intervention zone. When working on the sectional material, the distance between the distal portion of the ICA and the third segment of the VA was determined. It did not exceed, according to our data, 3.2+2.1 mm. This fact allows, if necessary, to safely form an anastomosis between the ICA and the VA in the V3 segment according to the «side-to-side» type. Conclusions. The proposed access to the third segment of the VA enables the reduction of surgical trauma by reducing the incision length up to 6.2+1.2 cm, as well as by mobilizing anatomical structures in the surgical wound without crossing them (salivary gland, jugular vein, vagus nerve, C2 - a portion of the brachial plexus, digastric muscle, internal carotid artery). With concomitant tortuosity of the ICA, the excess part of the ICA is the optimal material for bypassing the VA in the third segment of the VA. A variant of surgical correction in this situation is the formation of «trifurcation» of the carotid artery.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84282120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.7
Medvedeva L.M
Age-related macular degeneration (AMD) leads to a marked decrease in central vision and disrupts social and work activities of a person. This pathology is associated with age, and its clinically significant manifestations are determined in the age group from 66 to 74 years in 15% of the population, from 75 to 84 years - in 25%, from 85 years and older - in 30%. The pathogenesis of this disease is not fully understood. But the role of certain genes in the development of age-related macular degeneration has already been proved: PLEKHA1, HTRA1, CFH, CFB / C2, CRP, complement C3, ARMS2, HMCN1 / FBLN6, FBLN5, TLR3, ApoE, VEGF A and others. New genes, presumably related to AMD, continue to be investigated, for example, PLCG 2, which codes the enzyme phospholipase C. The only effective pathogenetic treatment of neovascular AMD nowadays is anti-VEGF therapy in the form of intravitreal injections. New promising areas of treatment appear: prolonged drugs (brolucizumab), combination therapy (inhibitors of the components of the complement system and anti-VEGF therapy), many of them being at various stages of clinical trials. Further study of the pathogenesis of AMD is aimed at the development of a new, more effective treatment.
{"title":"SOME ASPECTS OF PATHOGENESIS AND TREATMENT OF AGE-RELATED MACULAR DEGENERATION","authors":"Medvedeva L.M","doi":"10.22263/2312-4156.2021.5.7","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.7","url":null,"abstract":"Age-related macular degeneration (AMD) leads to a marked decrease in central vision and disrupts social and work activities of a person. This pathology is associated with age, and its clinically significant manifestations are determined in the age group from 66 to 74 years in 15% of the population, from 75 to 84 years - in 25%, from 85 years and older - in 30%. The pathogenesis of this disease is not fully understood. But the role of certain genes in the development of age-related macular degeneration has already been proved: PLEKHA1, HTRA1, CFH, CFB / C2, CRP, complement C3, ARMS2, HMCN1 / FBLN6, FBLN5, TLR3, ApoE, VEGF A and others. New genes, presumably related to AMD, continue to be investigated, for example, PLCG 2, which codes the enzyme phospholipase C. The only effective pathogenetic treatment of neovascular AMD nowadays is anti-VEGF therapy in the form of intravitreal injections. New promising areas of treatment appear: prolonged drugs (brolucizumab), combination therapy (inhibitors of the components of the complement system and anti-VEGF therapy), many of them being at various stages of clinical trials. Further study of the pathogenesis of AMD is aimed at the development of a new, more effective treatment.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86743337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.78
A. A. Kirpichenka, E. Martynova, F.A. Fomin
The results of the study dealing with the level of neurotization in patients with cholelithiasis in dynamics on the 1st-2nd days after surgery, in 6 months and in 5 years after surgery are presented. The aim of the work was to study the impact of intracrisis psychotherapeutic intervention on the recovery of patients with cholelithiasis in the near and long term. Already on the 1st-2nd days after the operation, a decrease in situational anxiety was detected in patients who had undergone psychotherapy, and after 6 months all indicators of anxiety-depression disorders did not differ from those of healthy people, while in patients without psychotherapy, the indicators of situational, personal anxiety and depression remained significantly higher than those in the control group of healthy people. In 5 years after discharge, the primary incidence of non-psychotic mental disorders in patients with cholelithiasis was 20.3%, and in patients who underwent intracrisis intervention - 2.7%. Thus, the implementation of intracrisis psychotherapeutic intervention improves the long-term results of treatment of patients with cholelithiasis.
{"title":"THE DYNAMICS OF NEUROTIZATION IN PATIENTS WITH CHOLELITHIASIS","authors":"A. A. Kirpichenka, E. Martynova, F.A. Fomin","doi":"10.22263/2312-4156.2021.5.78","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.78","url":null,"abstract":"The results of the study dealing with the level of neurotization in patients with cholelithiasis in dynamics on the 1st-2nd days after surgery, in 6 months and in 5 years after surgery are presented. The aim of the work was to study the impact of intracrisis psychotherapeutic intervention on the recovery of patients with cholelithiasis in the near and long term. Already on the 1st-2nd days after the operation, a decrease in situational anxiety was detected in patients who had undergone psychotherapy, and after 6 months all indicators of anxiety-depression disorders did not differ from those of healthy people, while in patients without psychotherapy, the indicators of situational, personal anxiety and depression remained significantly higher than those in the control group of healthy people. In 5 years after discharge, the primary incidence of non-psychotic mental disorders in patients with cholelithiasis was 20.3%, and in patients who underwent intracrisis intervention - 2.7%. Thus, the implementation of intracrisis psychotherapeutic intervention improves the long-term results of treatment of patients with cholelithiasis.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90227829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.49
D.V. Varikash, N.N. Dorokh, V. Bogdan
Objectives. To determine the prevalence and typology, as well as to establish the factors influencing the change of eating behavior in patients with morbid obesity. Material and methods. The method of continuous sampling was used to analyze the incidence of eating disorders types in 154 patients with morbid obesity who underwent planned surgical treatment for obesity at the Center for Herniology and Bariatric Surgery of the 4th City Clinical Hospital named after N.E. Savchenko during the period from 2016 to 2017. Additionally, a survey on the type of eating disorders was carried out in 81 patients with morbid obesity in the pre- and postoperative period after providing bariatric treatment. Results. The prevalence, typology of eating disorders associated with morbid obesity were studied, the assessment of the presence of correlation between body mass index (BMI) and the threshold value of eating disorders, as well as the assessment of changes of eating behavior in patients with morbid obesity in the postoperative period were made. Conclusions. Deviant forms of eating behavior are a specific criterion for morbid obesity with a dominant emocyogenic type of eating disorder with a body mass index of more than 60 kg / m2. Performing restrictive or combined bariatric surgery in patients with a restrictive type of eating disorder completely eliminates the existing eating disorder in 12 months after the operation, while maintaining a positive result in the long-term period.
{"title":"EATING DISORDERS IN MORBID OBESITY PATIENTS","authors":"D.V. Varikash, N.N. Dorokh, V. Bogdan","doi":"10.22263/2312-4156.2021.5.49","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.49","url":null,"abstract":"Objectives. To determine the prevalence and typology, as well as to establish the factors influencing the change of eating behavior in patients with morbid obesity. Material and methods. The method of continuous sampling was used to analyze the incidence of eating disorders types in 154 patients with morbid obesity who underwent planned surgical treatment for obesity at the Center for Herniology and Bariatric Surgery of the 4th City Clinical Hospital named after N.E. Savchenko during the period from 2016 to 2017. Additionally, a survey on the type of eating disorders was carried out in 81 patients with morbid obesity in the pre- and postoperative period after providing bariatric treatment. Results. The prevalence, typology of eating disorders associated with morbid obesity were studied, the assessment of the presence of correlation between body mass index (BMI) and the threshold value of eating disorders, as well as the assessment of changes of eating behavior in patients with morbid obesity in the postoperative period were made. Conclusions. Deviant forms of eating behavior are a specific criterion for morbid obesity with a dominant emocyogenic type of eating disorder with a body mass index of more than 60 kg / m2. Performing restrictive or combined bariatric surgery in patients with a restrictive type of eating disorder completely eliminates the existing eating disorder in 12 months after the operation, while maintaining a positive result in the long-term period.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84208324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.69
G.O. Usialionak, A. A. Kirpichenka, E. Martynova, A. F. Martsinkevich
The application of objective, high-precision and instrumental methods for diagnosing human psyche continues to be a pressing problem in modern psychiatry, narcology and other sciences. The rapid development of modern technology, including mobile phones, tablets and computers, makes it possible to port classical neuropsychological methods to mobile devices, thus simplifying the process of diagnosis and analysis of its results. The tapping test is a simple and easy-to-reproduce method of rapid diagnosing of nervous system properties by psychomotor indices. The classical method of the tapping test is the estimation of the number of marks given to the subjects on the selected piece of paper in the allotted time by the right and left hand. The given test has proved itself well, and later more sophisticated touch tracking devices appeared, including mechanical and electronic ones, which allow registering not only the number of touches, but also their duration, speed and strength. At the same time, despite the increased analytical significance, the carrying out of the automated tapping test is associated with significant difficulties, justified, first of all, by low portability, high cost, and low prevalence of the said equipment. This article describes the development of an application of E.P. Ilyin tapping test for the Android operating system. Tapping test is a classic technique for diagnosing the properties of the nervous system by psychomotor indicators, which determines the strength of the subject’s nervous system. In the course of the application work, in addition to determining the coefficient of strength of the nervous system, a number of psychomotor characteristics are additionally investigated: the coefficient of functional asymmetry in terms of the working capacity of the left and right hands, the interval between touches in milliseconds, the duration of the touch, the speed of the touch, the number of touches per second, the cumulative sum of touches per second , the density of touches during the tapping test. The functionality of the application and the testing methodology are also described.
{"title":"THE USE OF NEUROSMG:FOT APPLICATION FOR CARRYING OUT TAPPING TEST","authors":"G.O. Usialionak, A. A. Kirpichenka, E. Martynova, A. F. Martsinkevich","doi":"10.22263/2312-4156.2021.5.69","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.69","url":null,"abstract":"The application of objective, high-precision and instrumental methods for diagnosing human psyche continues to be a pressing problem in modern psychiatry, narcology and other sciences. The rapid development of modern technology, including mobile phones, tablets and computers, makes it possible to port classical neuropsychological methods to mobile devices, thus simplifying the process of diagnosis and analysis of its results. The tapping test is a simple and easy-to-reproduce method of rapid diagnosing of nervous system properties by psychomotor indices. The classical method of the tapping test is the estimation of the number of marks given to the subjects on the selected piece of paper in the allotted time by the right and left hand. The given test has proved itself well, and later more sophisticated touch tracking devices appeared, including mechanical and electronic ones, which allow registering not only the number of touches, but also their duration, speed and strength. At the same time, despite the increased analytical significance, the carrying out of the automated tapping test is associated with significant difficulties, justified, first of all, by low portability, high cost, and low prevalence of the said equipment. This article describes the development of an application of E.P. Ilyin tapping test for the Android operating system. Tapping test is a classic technique for diagnosing the properties of the nervous system by psychomotor indicators, which determines the strength of the subject’s nervous system. In the course of the application work, in addition to determining the coefficient of strength of the nervous system, a number of psychomotor characteristics are additionally investigated: the coefficient of functional asymmetry in terms of the working capacity of the left and right hands, the interval between touches in milliseconds, the duration of the touch, the speed of the touch, the number of touches per second, the cumulative sum of touches per second , the density of touches during the tapping test. The functionality of the application and the testing methodology are also described.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85257513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.22263/2312-4156.2021.5.23
Lazuko S.S
Objectives. To assess the possibility of preventing the disturbances of coronary vascular tone and myocardial contractile function caused by diabetes mellitus with the help of 2-ethylthiobenzimidazole hydrobromide. Material and methods. Coronary vascular tone and myocardial contractile function were studied on preparations of rat hearts isolated by the Langendorff method. The iNOS blockade was carried out with S-methylisothiourea (S-MT, 10-6M). Diabetes mellitus in rats was modelled by means of a single intraperitoneal injection of streptozocin (50 mg / kg). 2-ethylthiobenzimidazole hydrobromide (2-ETG) was injected intraperitoneally at a dose of 3 mg / kg. The concentration of stable degradation products of NO (NO2-/NO3-), superoxide dismutase, catalase, diene conjugates and malondialdehyde in the left ventricular homogenate was determined by spectrophotometric method. The content of inducible and endothelial NO-synthases (eNOS), interleukin 1β, C-reactive protein in the blood serum of experimental animals was determined by enzyme immunoassay. Results. In the hearts of «2-ETG + Diabetes mellitus» animal group no changes in the coronary perfusion pressure and developed intraventricular pressure were observed before and after the use of the highly selective iNOS blocker S-methylisothiourea. In the blood serum of these animals group, an increase in the concentration of eNOS was observed, against the background of a decrease in the accumulation of iNOS, a decrease in the concentration of lipid peroxidation products was determined against the background of an increase in the activity of the antioxidant system and a decrease of systemic inflammation. Conclusions. Intraperitoneal injection of 2-ethylthiobenzimidazole hydrobromide prevents a decrease in coronary vascular tone and myocardial contractile function caused by hyperproduction of nitrogen monoxide of inducible NO-synthase in diabetes mellitus. This effect of 2-ethylthiobenzimidazole hydrobromide is associated with: limiting the formation of oxidative stress; limiting the nitrosative stress; the decrease in the concentration of inflammatory markers.
{"title":"2-ETHYLTHIOBENZIMIDAZOLE HYDROBROMIDE PREVENTS A DECREASE IN CORONARY VASCULAR TONE AND MYOCARDIAL CONTRACTILITY IN RATS WITH EXPERIMENTAL DIABETES MELLITUS","authors":"Lazuko S.S","doi":"10.22263/2312-4156.2021.5.23","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.5.23","url":null,"abstract":"Objectives. To assess the possibility of preventing the disturbances of coronary vascular tone and myocardial contractile function caused by diabetes mellitus with the help of 2-ethylthiobenzimidazole hydrobromide. Material and methods. Coronary vascular tone and myocardial contractile function were studied on preparations of rat hearts isolated by the Langendorff method. The iNOS blockade was carried out with S-methylisothiourea (S-MT, 10-6M). Diabetes mellitus in rats was modelled by means of a single intraperitoneal injection of streptozocin (50 mg / kg). 2-ethylthiobenzimidazole hydrobromide (2-ETG) was injected intraperitoneally at a dose of 3 mg / kg. The concentration of stable degradation products of NO (NO2-/NO3-), superoxide dismutase, catalase, diene conjugates and malondialdehyde in the left ventricular homogenate was determined by spectrophotometric method. The content of inducible and endothelial NO-synthases (eNOS), interleukin 1β, C-reactive protein in the blood serum of experimental animals was determined by enzyme immunoassay. Results. In the hearts of «2-ETG + Diabetes mellitus» animal group no changes in the coronary perfusion pressure and developed intraventricular pressure were observed before and after the use of the highly selective iNOS blocker S-methylisothiourea. In the blood serum of these animals group, an increase in the concentration of eNOS was observed, against the background of a decrease in the accumulation of iNOS, a decrease in the concentration of lipid peroxidation products was determined against the background of an increase in the activity of the antioxidant system and a decrease of systemic inflammation. Conclusions. Intraperitoneal injection of 2-ethylthiobenzimidazole hydrobromide prevents a decrease in coronary vascular tone and myocardial contractile function caused by hyperproduction of nitrogen monoxide of inducible NO-synthase in diabetes mellitus. This effect of 2-ethylthiobenzimidazole hydrobromide is associated with: limiting the formation of oxidative stress; limiting the nitrosative stress; the decrease in the concentration of inflammatory markers.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77761241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-17DOI: 10.22263/2312-4156.2021.4.38
A. Tkachenko, Volodymyr Prokopiuk, A. Onishchenko
Objectives. To study the effects of various concentrations of the food additive E407a (semi-refined carrageenan) on the metabolic activity of fetal liver cells, splenocytes, and bone marrow cells. Material and methods. Fetal liver, splenocytes and bone marrow cell cultures were incubated with the food additive E407a at concentrations varying from 0 mg/ml to 10 mg/ml for 24 hours (n=8). To analyze the effects of this food additive on the metabolic activity of cells, a colorimetric MTT assay was used. It is based on the ability of viable, metabolically active cells to convert 3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide into formazan. The data were statistically processed using Kruskal-Wallis and Dunn’s criteria. Results. The bone marrow cell culture was found to be the most sensitive to carrageenan. More than a twofold statistically significant (p<0.0001) increase in the metabolic activity of bone marrow cells was observed when using E407a from 200 μg/ml and above. The metabolic activity of splenocytes increased approximately 1.5 times and over (p<0.0001) when using carrageenans at the concentration of 500 μg/ml and higher. Fetal liver cells turned out to be the most resistant to the direct toxic effect of the food additive E407a. Conclusions. The food additive E407a is cytotoxic to bone marrow cells and splenocytes at concentrations of 200 μg/ml and 500 μg/ml, respectively.
{"title":"THE IMPACT OF THE FOOD ADDITIVE E407A ON THE METABOLIC ACTIVITY OF DIFFERENT CELL CULTURES","authors":"A. Tkachenko, Volodymyr Prokopiuk, A. Onishchenko","doi":"10.22263/2312-4156.2021.4.38","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.4.38","url":null,"abstract":"Objectives. To study the effects of various concentrations of the food additive E407a (semi-refined carrageenan) on the metabolic activity of fetal liver cells, splenocytes, and bone marrow cells. Material and methods. Fetal liver, splenocytes and bone marrow cell cultures were incubated with the food additive E407a at concentrations varying from 0 mg/ml to 10 mg/ml for 24 hours (n=8). To analyze the effects of this food additive on the metabolic activity of cells, a colorimetric MTT assay was used. It is based on the ability of viable, metabolically active cells to convert 3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide into formazan. The data were statistically processed using Kruskal-Wallis and Dunn’s criteria. Results. The bone marrow cell culture was found to be the most sensitive to carrageenan. More than a twofold statistically significant (p<0.0001) increase in the metabolic activity of bone marrow cells was observed when using E407a from 200 μg/ml and above. The metabolic activity of splenocytes increased approximately 1.5 times and over (p<0.0001) when using carrageenans at the concentration of 500 μg/ml and higher. Fetal liver cells turned out to be the most resistant to the direct toxic effect of the food additive E407a. Conclusions. The food additive E407a is cytotoxic to bone marrow cells and splenocytes at concentrations of 200 μg/ml and 500 μg/ml, respectively.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72702036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-17DOI: 10.22263/2312-4156.2021.4.67
V. Novitskaya, S. E. Rzheussky
The purpose of this article was to analyze the pharmaceutical market of the tissue sealing agents group of the Republic of Belarus from 2010 to 2018 based on the data presented in the Medmarket database and the State Register of Medicines of the Republic of Belarus of UE «Center of Expertise and Testing in Healthcare». The group of tissue sealing agents (B02F) was found to be characterized by high growth rates and a relatively large market share. During the studied period, it was represented on the Belarusian pharmaceutical market by only one drug - Tachocomb (manufactured by Takeda Pharmaceutical Company Limited, Japan). When analyzing its dynamics in the period from 2010 to 2018 it was found that, in general, the change in the volume of the market for tissue sealing agents correlates with the change in the volume of the total market for hemostatic drugs over the years. During 2010-2018 there is an increase in the volume of sales for this local sealant, both in monetary (by 1.51) and in natural (by 2.01) expressions with a gradual fall of the price. It has been concluded that the development of the tissue sealing agents by domestic manufacturers is urgent and expedient.
{"title":"TISSUE SEALING MEDICINAL AGENTS GROUP ON THE PHARMACEUTICAL MARKET OF THE REPUBLIC OF BELARUS","authors":"V. Novitskaya, S. E. Rzheussky","doi":"10.22263/2312-4156.2021.4.67","DOIUrl":"https://doi.org/10.22263/2312-4156.2021.4.67","url":null,"abstract":"The purpose of this article was to analyze the pharmaceutical market of the tissue sealing agents group of the Republic of Belarus from 2010 to 2018 based on the data presented in the Medmarket database and the State Register of Medicines of the Republic of Belarus of UE «Center of Expertise and Testing in Healthcare». The group of tissue sealing agents (B02F) was found to be characterized by high growth rates and a relatively large market share. During the studied period, it was represented on the Belarusian pharmaceutical market by only one drug - Tachocomb (manufactured by Takeda Pharmaceutical Company Limited, Japan). When analyzing its dynamics in the period from 2010 to 2018 it was found that, in general, the change in the volume of the market for tissue sealing agents correlates with the change in the volume of the total market for hemostatic drugs over the years. During 2010-2018 there is an increase in the volume of sales for this local sealant, both in monetary (by 1.51) and in natural (by 2.01) expressions with a gradual fall of the price. It has been concluded that the development of the tissue sealing agents by domestic manufacturers is urgent and expedient.","PeriodicalId":23571,"journal":{"name":"Vestnik of Vitebsk State Medical University","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79997915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：