Veterinary clinical pathology最新文献_第7页

Analytical and Quality Control Validation of a Novel Symmetric Dimethylarginine Assay in Dogs and Cats 新型对称二甲基精氨酸在狗和猫中的分析和质量控制验证。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-18 DOI: 10.1111/vcp.70037

Portia Tshidi Mashego, Emma H. Hooijberg

Background

An immunoturbidometric assay for symmetric dimethylarginine (SDMA) measurement on automated chemistry analyzers has recently become available.

Objective

To perform analytical validation of the EUROLyser SDMA assay in dogs and cats.

Methods

Method validation experiments were performed using stored canine and feline serum. Quality control validation was performed according to Westgard. Performance goals were derived from SDMA biological variation data for both species.

Results

Imprecision ranged from 3.7%–7.8% (dogs) to 6.0%–11.8% (cats) with a dispersion of ±35% for dogs and ±44% for cats. The assay showed linearity (up to 85 μg/dL [dogs], 75 μg/dL [cats]); the preliminary LoQ was 9.5 and 6.9 μg/dL, respectively. Recovery was 19.7% and 6.5%, respectively. Severe hemolysis resulted in a significant bias in both species. The EUROLyser method showed a significant negative proportional and constant bias in dogs and a significant positive proportional and negative constant bias in cats, compared to the comparative method. In dogs, the mean bias (−19.5%) and the bias at clinical decision limits exceeded the desirable bias; in cats, the mean bias (−4.0%) and the bias at clinical decision limits was < 8%. Because of high imprecision, the TE_A that could be controlled for was 35%, with a 1–2.5 s rule using patient pools as quality control material.

Conclusions

The novel SDMA assay showed acceptable analytical performance, but high dispersion has consequences for the interpretation of results at reference limits and serial measurements. Method-specific reference intervals and decision limits should be generated for both species, particularly for dogs, given the significant bias vs. the comparative method.

背景：一种在自动化化学分析仪上测量对称二甲基精氨酸（SDMA）的免疫浊度测定方法最近变得可行。目的：对EUROLyser SDMA法在狗和猫中的应用进行分析验证。方法：用储存的犬、猫血清进行方法验证实验。根据Westgard进行质量控制验证。性能目标来源于两个物种的SDMA生物变异数据。结果：不精确性范围为3.7% ~ 7.8%（狗）~ 6.0% ~ 11.8%（猫），分散度为±35%（狗）和±44%（猫）。检测结果呈线性（最高85 μg/dL[狗]，75 μg/dL[猫]）；初步定量限分别为9.5和6.9 μg/dL。回收率分别为19.7%和6.5%。严重的溶血导致两个物种的显著偏倚。与比较法相比，EUROLyser方法在狗中显示出显著的负比例和恒定偏差，在猫中显示出显著的正比例和负恒定偏差。在狗中，平均偏差（-19.5%）和临床决策极限偏差超过了理想偏差；在猫中，平均偏倚（-4.0%）和临床决策极限的偏倚为A，可控制的偏倚为35%，使用患者池作为质量控制材料的1-2.5 s规则。结论：新型SDMA分析具有可接受的分析性能，但高分散会影响参考限和连续测量结果的解释。考虑到与比较方法的显著偏差，应该为这两个物种，特别是狗，产生特定于方法的参考区间和决策限制。

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引用次数: 0

What's Your Diagnosis? A Case of Extreme Thrombocytosis in a Dog. 你的诊断是什么？犬重度血小板增多症1例。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-17 DOI: 10.1111/vcp.70022

Stephanie F Anderson, King Long Hannah Lee, Megan Dietz, Sarah Guess, Laura White, Mara S Varvil

引用次数: 0

Comparative Testing of In-Clinic, Point-of-Care Hematology Analyzers With a Commercial Laboratory Reference Analyzer 临床比较测试，点护理血液学分析仪与商业实验室参考分析仪。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-17 DOI: 10.1111/vcp.70023

S. Daly, K. P. Freeman, P. A. Graham

Background

Previous studies have shown that veterinary in-clinic hematology analyzers may have analytical errors, but few studies have been found that attempt bias correction and look at the effect of this on the achievement of several quality goals and evaluation of analytical performance specifications.

Objectives

To design a comparative testing program for use by in-clinic laboratories with a variety of point-of-care (POC) analyzers and to evaluate the performance of in-clinic hematology analyzers compared to a reference analyzer. To determine whether the removal of bias from the in-clinic result can be used in preparation for the harmonization of the results with those of the reference laboratory. To determine the analytical performance specifications/quality goals that can be achieved with the removal of bias from the in-clinic results. To determine whether clinic-based clinicians and technicians found the information provided valuable for ongoing in-clinic hematology quality management.

Methods

Fresh EDTA whole blood canine samples with results that were within reference limits were processed five times by in-clinic staff using their in-clinic analyzer before sending the sample to the reference laboratory for comparison. Results were assessed using quality goals, including total allowable error (TE_a) or expert opinion and sigma metrics. The quality goal index (QGI) was calculated for measurands whose performance was < 4 sigma. The potential impact of any difference in results on clinical interpretation was also reviewed.

Results

In comparison to the reference analyzer, in-clinic hematology analyzers often had analytical error and failed to meet quality goals for TE_a and sigma metrics. This error was often undetected by the in-clinic laboratory. Sigma metric < 4 was a useful performance indicator of poor performance, and only 54% of measurands had sigma metrics > 4. Of these poor-performing instances, 90% were attributed to bias. Performance improved for in-clinic analyzers by adjusting results to correct for bias in preparation for harmonization.

Conclusion

A comparative testing program was successfully designed and implemented to evaluate performance and harmonize in-clinic hematology analyzers to a reference analyzer using samples that are within reference intervals.

背景：以往的研究表明，兽医临床血液学分析仪可能存在分析误差，但很少有研究发现试图纠正偏差，并观察其对实现几个质量目标和评估分析性能规范的影响。目的：设计一个临床实验室与各种护理点（POC）分析仪使用的比较测试程序，并评估临床血液学分析仪与参考分析仪的性能。确定临床结果偏差的消除是否可用于准备与参比实验室的结果相协调。通过消除临床结果的偏倚，确定可以实现的分析性能规范/质量目标。确定临床医生和技术人员是否发现这些信息对正在进行的临床血液学质量管理有价值。方法：新鲜EDTA全血犬标本经临床工作人员用临床分析仪处理5次后，送参比实验室进行比对。使用质量目标评估结果，包括总允许误差（TEa）或专家意见和西格玛指标。结果：与参考分析仪相比，临床血液学分析仪经常存在分析误差，未能达到TEa和sigma指标的质量目标。这种错误通常不会被临床实验室发现。度量4。在这些表现不佳的案例中，90%归因于偏见。通过调整结果以纠正在协调准备中的偏差，提高了临床分析仪的性能。结论：成功地设计和实施了一个比较测试程序，以评估性能并协调临床血液学分析仪与参考分析仪使用参考区间内的样本。

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引用次数: 0

Diagnostic Sensitivity of the PCR for Antigen Receptor Rearrangement (PARR) Assay for Canine Plasma Cell Tumors 抗原受体重排（PARR） PCR检测犬浆细胞瘤的诊断敏感性

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-15 DOI: 10.1111/vcp.70036

Emily D. Rout, Kacie Seymour, Robert Burnett, Cora Contreras, Anne C. Avery, A Russell Moore

Background

The PCR for Antigen Receptor Rearrangement (PARR) assay assesses clonality of a lymphoid population. Studies suggest PARR has decreased sensitivity for detecting clonality in canine plasma cell tumors (PCT) compared to lymphoma.

Objective

Assess sensitivity of PARR assays targeting the immunoglobulin heavy chain (IGH), immunoglobulin lambda light chain (IGL), and kappa deleting element (Kde) loci in canine PCT.

Methods

Canine cases included 35 PCTs (multiple myeloma and extramedullary, cutaneous or oral PCTs), 5 non-PCT foil cases, and 40 B-cell lymphoma cases. PCT diagnoses were confirmed via CD3, PAX5, and MUM1 immunolabeling, and some additionally had histopathology, serum or urine protein electrophoresis, or immunofixation. Nodal B-cell lymphomas were diagnosed by flow cytometry. Routine PARR (targeting IGH V-D-J and D-J rearrangements) and extended PARR (targeting additional IGH genes and IGL and Kde rearrangements) were performed on cytologic specimens (PCT and foil cases) and flow cytometry aspirates (lymphoma cases). Diagnostic sensitivity was calculated.

Results

Across both PARR assays, every PCT case with sufficient sample was interpreted as clonal and no foil cases were clonal. The routine PARR assay detected a clonal immunoglobulin rearrangement in 26/35 PCT cases (74.3%; 95% CI 56.7%–87.5%). The extended assay detected immunoglobulin gene clonality in 33/35 PCT cases (94.3%; 95% CI 80.8%–99.3%). 40/40 lymphoma cases were clonal in both the routine and extended PARR assays.

Conclusion

The combined PARR assays used in this study, evaluating multiple IG loci, detected clonality in all PCTs tested, but routine PARR (targeting IGH) was less sensitive for detecting PCT clonality compared to B-cell lymphomas.

背景：抗原受体重排（PARR） PCR检测评估淋巴细胞群体的克隆性。研究表明，与淋巴瘤相比，PARR在犬浆细胞肿瘤（PCT）中检测克隆性的敏感性降低。目的：评价免疫球蛋白重链（IGH）、免疫球蛋白轻链（IGL）和卡帕删除元件（Kde）位点在犬pct中的敏感性。方法：35例犬pct（多发性骨髓瘤和髓外、皮肤或口腔pct）， 5例非pct foil病例和40例b细胞淋巴瘤。PCT诊断通过CD3、PAX5和MUM1免疫标记得到证实，部分患者另外进行组织病理学、血清或尿蛋白电泳或免疫固定。流式细胞术诊断淋巴结b细胞淋巴瘤。常规PARR（靶向IGH V-D-J和D-J重排）和扩展PARR（靶向额外的IGH基因和IGL和Kde重排）对细胞学标本（PCT和箔病例）和流式细胞术抽吸物（淋巴瘤病例）进行。计算诊断灵敏度。结果：在两种PARR分析中，每一个有足够样本的PCT病例都被解释为克隆，没有箔病例被解释为克隆。常规PARR检测在26/35例PCT病例中检测到克隆性免疫球蛋白重排(74.3%；95% ci 56.7%-87.5%)。扩展试验在33/35例PCT病例中检测到免疫球蛋白基因克隆(94.3%；95% ci 80.8%-99.3%)。40/40的淋巴瘤病例在常规和扩展PARR检测中均为克隆。结论：本研究中使用的联合PARR分析评估了多个IG位点，检测了所有PCT的克隆性，但与b细胞淋巴瘤相比，常规PARR（靶向IGH）检测PCT克隆性的敏感性较低。

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引用次数: 0

Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in Cats 方法手持式猫血红蛋白仪（HemoCue Hb 201+）干扰变量的比较与研究。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-15 DOI: 10.1111/vcp.70035

Yuqing Sun, Xiu Ting Yiew, Janet Beeler-Marfisi, Carolyn L. Kerr, Gabrielle Monteith, Shane Bateman

Background

Regular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201⁺ (HC-201), requires only one drop of blood (10 μL) and provides results within 1 min.

Objectives

This preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.

Methods

One hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2–3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland–Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.

Results

Passing-Bablok analysis revealed a significant constant bias (intercept = −2.242, 95% CI: −4.042 to −0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000–1.032). HC-201 demonstrated excellent agreement (ρ_c = 0.989) and precision (ICC = 0.997) with a median bias of −0.67 g/L (p = 0.001). The total observed error was 3.02%, within the allowable limits defined by international standards. Neither anemia, leukocytosis, azotemia, lipemia, nor venipuncture site influenced HC-201 measurements. Samples with icterus and hemolysis were insufficient in number for statistical comparison.

Conclusions

This preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.

背景：定期抽血监测贫血猫的红细胞质量会加重贫血。基于实验室的报告可能会延迟临床决策。手持血红蛋白仪HemoCue Hb 201+ (HC-201)只需一滴血（10 μL），并在1分钟内提供结果。目的：本初步研究旨在评估HC-201在猫中的效用，并探讨潜在干扰物对其性能的影响。方法：对93只猫154份静脉血EDTA进行分析。使用ADVIA 2120分析仪测量一次血红蛋白浓度，并与HC-201的2-3次重复测量的平均值进行比较。采用Lin’s一致性相关系数、非参数Bland-Altman、Passing-Bablok回归和类内相关系数评估HC-201和ADVIA结果的一致性和系统偏倚，以及HC-201重复之间的精度。采用Wilcoxon秩和检验评估HC-201在贫血、白细胞减少、氮质血症、血脂、黄疸、溶血以及外周静脉穿刺与颈静脉穿刺时的表现。结果：Passing-Bablok分析显示显著的常数偏差（截距= -2.242,95% CI: -4.042至-0.667），但无显著的比例偏差（斜率= 1.015,95% CI: 1.000-1.032）。HC-201具有良好的一致性（ρc = 0.989）和精密度（ICC = 0.997），中位偏差为-0.67 g/L （p = 0.001）。总观测误差为3.02%，在国际标准允许范围内。贫血、白细胞、氮质血症、血脂和静脉穿刺部位均不影响HC-201的测量。伴有黄疸和溶血的样本数量不足，无法进行统计学比较。结论：这项初步研究表明，HC-201为猫的血红蛋白浓度提供了可靠的护理点监测。

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引用次数: 0

HARISS: Histogram Analyzer for Reference Intervals of Small Samples, a Free Web App to Calculate Reference Intervals of Small Samples. HARISS：小样本参考区间的直方图分析仪，一个免费的Web应用程序来计算小样本的参考区间。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-14 DOI: 10.1111/vcp.70033

Kevin Le Boedec

Background: Reference interval (RI) estimate inaccuracy is problematic at small sample sizes. Visual assessment of distribution histograms (VADH) may improve statistical technique selection, but its performance depends on the human operator.

Objectives: This study aimed to develop a machine learning model for small-sample VADH.

Methods: The training set consisted of 45 000 distribution histograms from samples ranging from 20 to 40 individuals extracted from simulated Gaussian, lognormal, and left-skewed populations. A convolutional neural network (CNN) was trained to predict the original population distribution by VADH. Its accuracy was tested on 900 human-classified histograms from a previous study (ranging from 20 to 60 individuals) and compared to the Shapiro-Wilk test performance in determining the original population distribution. A web application was developed to allow usage of the CNN, 95% RI estimation with 90% confidence intervals (CI) via bootstrapping, and outlier detection.

Results: The CNN model properly predicted the original population distribution by VADH in 84.0% (95% CI: 83.7-84.4) and 94.4% (95% CI: 92.7-95.8) of the samples of the training and the test sets, respectively. Comparatively, on the test set, the Shapiro-Wilk test accuracy was 65.0% (95% CI: 61.8-68.1) and 72.3% (95% CI: 69.3-75.2) using a p-value threshold of 0.05 and 0.2, respectively. The web application (named HARISS) was successfully deployed and is accessible at: https://hariss.streamlit.app/.

Conclusions: The CNN model demonstrated effective VADH and might enhance RI estimate accuracy through the use of the HARISS web app, though proper reference individual selection and preanalytical/analytical conditions remain paramount.

背景：在小样本量下，参考区间（RI）估计不准确是有问题的。分布直方图的视觉评估（VADH）可以改善统计技术的选择，但其性能取决于人工操作者。目的：本研究旨在建立小样本VADH的机器学习模型。方法：训练集包括从模拟高斯、对数正态和左偏总体中提取的20 - 40个样本的45,000个分布直方图。通过VADH训练卷积神经网络（CNN）来预测原始种群分布。它的准确性在之前的一项研究（从20到60个个体）的900个人类分类直方图上进行了测试，并与夏皮罗-威尔克测试在确定原始种群分布方面的表现进行了比较。开发了一个web应用程序，允许使用CNN， 95% RI估计和90%置信区间（CI），通过自举和离群值检测。结果：CNN模型在训练集样本和测试集样本中分别有84.0% （95% CI: 83.7 ~ 84.4）和94.4% （95% CI: 92.7 ~ 95.8）能正确预测原始总体分布。相比之下，在测试集上，Shapiro-Wilk测试准确率分别为65.0% （95% CI: 61.8-68.1）和72.3% (95% CI: 69.3-75.2)， p值阈值分别为0.05和0.2。web应用程序（名为HARISS）已成功部署，并可访问：https://hariss.streamlit.app/.Conclusions: CNN模型展示了有效的VADH，并可能通过使用HARISS web应用程序提高RI估计的准确性，尽管适当的参考个体选择和分析前/分析条件仍然是至关重要的。

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引用次数: 0

Development of Reference Intervals for the TEG 6s, a Point-of-Care Viscoelastic Assay, in Dogs TEG 6s参考区间的发展，一个护理点粘弹性测定，在狗。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-14 DOI: 10.1111/vcp.70027

Ashley R. Wilkinson, Bobbi J. Conner, Priscila B. S. Serpa

Background

Thromboelastography (TEG) provides a global assessment of hemostasis and identifies patients with both hemorrhagic and thrombotic tendencies. Traditional TEG instruments, such as the TEG 5000 (Haemonetics), can be labor-intensive and require specialized training to operate. The TEG 6s (Haemonetics) is a commercially available point-of-care TEG that employs a cartridge-based system. Agreement between the TEG 6s and TEG 5000 is poor in dogs, and their viscoelastic measurements are not interchangeable. Reference intervals specific to the TEG 6s are needed for healthy adult dogs.

Objectives

This study aimed to establish reference intervals for the TEG 6s in healthy adult dogs.

Methods

Citrated whole blood samples collected from 40 healthy adult dogs were analyzed using the TEG 6s and associated global hemostasis and lysis cartridges. Reference intervals were established using a nonparametric statistical method for all variables except for the Functional Fibrinogen level in the global hemostasis cartridge.

Results

Reference intervals were established for 12 TEG 6s measurands in healthy adult dogs.

Conclusions

The results of this study provide reference intervals for TEG 6s variables associated with the global hemostasis and lysis cartridges in healthy adult dogs. Further studies are required to assess TEG 6s' utility in dogs with various disease states.

背景：血栓弹性成像（TEG）提供了一种全面的止血评估，并识别出出血和血栓倾向的患者。传统的TEG仪器，如TEG 5000 (Haemonetics)，可能是劳动密集型的，需要专门的培训才能操作。TEG 6s （Haemonetics）是一种市售的即时护理TEG，采用了基于墨盒的系统。TEG 6s和TEG 5000之间的一致性在狗身上很差，而且它们的粘弹性测量是不可互换的。对于健康的成年犬来说，TEG 6需要有特定的参考间隔。目的：本研究旨在建立健康成年犬TEG 6s的参考区间。方法：采集40只健康成年犬柠檬酸全血，采用TEG - 6s及相关的全血止血和溶解试剂盒进行分析。参考区间采用非参数统计方法对所有变量建立，除了整体止血药筒中的功能性纤维蛋白原水平。结果：建立了健康成年犬12项TEG - 6s指标的参考区间。结论：本研究结果为健康成年犬全身止血和溶栓栓相关的TEG 6s变量提供了参考区间。需要进一步的研究来评估TEG - 6s在不同疾病状态的狗中的效用。

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引用次数: 0

A Stab in the Dark 《暗箭伤人》

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-07 DOI: 10.1111/vcp.70045

Rachel E. Whitman, Francisco O. Conrado

引用次数: 0

Biochemical Reference Intervals of Free-Ranging Koalas (Phascolarctos cinereus) in South Australia 南澳大利亚自由放养考拉（Phascolarctos cinereus）的生化参考区间。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-07-02 DOI: 10.1111/vcp.70024

María B. Font, Lucy Woolford, Sue Jaensch, Doug Hayward, Michelle Hebart, Emily Dean, Wayne S. J. Boardman, Tamsyn Stephenson, Jessica Kovac, Natasha Speight

Background

Reference intervals (RIs) are an essential tool for assessment of clinical pathology data of animals, and are particularly important for monitoring the health status of free-ranging and captive wildlife, such as koalas (Phascolarctos cinereus).

Objectives

The purpose of this study was to: (1) provide comprehensive serum biochemistry reference intervals based on clinically healthy South Australian koalas from two populations, Mount Lofty Ranges (MLR) and Kangaroo Island (KI); and (2) identify any factors that can affect biochemical analytes, including koala retrovirus (KoRV) and Chlamydia pecorum subclinical infection status, age, sex, and population.

Methods

Serum biochemistry analytes were determined in 206 clinically healthy South Australian koalas caught from the wild in 2016 and 2018 using a Cobas 8000 Chemistry Analyzer and analyzed using Reference Value Advisor and SPSS v28 Statistical software.

Results

Biochemical reference intervals were established. Also, clinically and statistically significant differences in analytes were found based on age for alkaline phosphatase and phosphate, and albumin: globulin ratio, globulins, and total protein, most likely associated with physiological bone growth and immunological development, respectively, as observed in other species. Statistically significant differences between animals subclinically positive for KoRV and Chlamydia pecorum, were found for glucose and gamma glutamyl transferase respectively; however, these were marginal, and their reference intervals were similar.

Conclusions

This study is the first to describe serum biochemical reference intervals for clinically healthy South Australian koalas of known Chlamydia and KoRV infection status. It represents an important tool to assist health assessments of koalas by veterinarians, as well as research and population monitoring.

背景：参考区间（RIs）是评估动物临床病理数据的重要工具，对于监测自由放养和圈养野生动物（如考拉）的健康状况尤其重要。目的：本研究的目的是：(1)为临床健康的南澳大利亚考拉（Mount Lofty Ranges， MLR）和袋鼠岛（Kangaroo Island， KI）两个种群提供全面的血清生化参考区间；(2)确定任何可能影响生化分析的因素，包括考拉逆转录病毒（KoRV）和pecorum衣原体亚临床感染状况、年龄、性别和人群。方法：使用Cobas 8000化学分析仪对2016年和2018年野外捕获的206只临床健康的南澳大利亚考拉进行血清生化分析，并使用参考值顾问和SPSS v28统计软件进行分析。结果：建立了生化参考区间。此外，在其他物种中观察到，基于年龄的碱性磷酸酶和磷酸盐，白蛋白：球蛋白比率，球蛋白和总蛋白，最有可能分别与生理骨骼生长和免疫发育相关，在分析物中发现临床和统计学上显著差异。KoRV亚临床阳性和pecorum衣原体亚临床阳性动物的葡萄糖和谷氨酰转移酶差异有统计学意义；然而，这些都是边缘的，它们的参考区间是相似的。结论：本研究首次描述了已知衣原体和KoRV感染状态的临床健康南澳大利亚考拉的血清生化参考区间。它是一种重要的工具，可以帮助兽医对考拉进行健康评估，以及进行研究和种群监测。

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引用次数: 0

Transient Leukemia in an Adult Dog With Cutaneous T-Cell Lymphoma 成年犬皮肤t细胞淋巴瘤的短暂性白血病。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-16 DOI: 10.1111/vcp.70025

Raquel Moreira, Malgorzata Ossowska, Oriol Jornet-Rius, Marta Santos, Francesco Cian

A 5-year-old neutered female Beagle was presented with lethargy, vomiting, and reduced appetite. Physical examination revealed mild hyperthermia and a cutaneous thickness on the right thigh. Hematology showed marked leukocytosis and moderate thrombocytopenia, consisting of 80% of atypical circulating cells, initially suggesting acute undifferentiated leukemia. Two weeks later, repeated hematology revealed unremarkable results. Cytology of the skin lesion showed a round cell neoplasia with features similar to the atypical circulating cells. Following the development of multiple cutaneous nodules and recurrence of clinical signs, further diagnostics, including flow cytometry, histopathology, and immunohistochemistry (IHC) of the skin nodules, as well as PCR for antigen receptor rearrangement (PARR) and immunocytochemistry (ICC) from the initial blood smear, confirmed a neoplastic T-cell proliferation consistent with cutaneous T-cell lymphoma with a probable transient leukemic phase. Despite chemotherapy, remission was short-lived, and the patient relapsed, ultimately leading to euthanasia. This case highlights a rare instance of transient leukemia likely originating from a primary cutaneous T-cell lymphoma, emphasizing the need for comprehensive diagnostic workups, combining hematology, biochemistry, cytology, flow cytometry, and immunophenotyping to avoid misclassification in hematologic malignancies.

一只5岁的绝育雌比格犬表现为嗜睡、呕吐和食欲减退。体格检查显示轻度高热，右大腿皮肤增厚。血液学显示明显的白细胞增多和中度血小板减少，包括80%的非典型循环细胞，初步提示急性未分化白血病。两周后，重复血液学检查显示无明显结果。皮肤病变的细胞学表现为圆形细胞瘤，其特征与非典型循环细胞相似。随着多发性皮肤结节的发展和临床症状的复发，进一步的诊断，包括流式细胞术、组织病理学和皮肤结节的免疫组织化学（IHC），以及抗原受体重排（PARR）的PCR和免疫细胞化学（ICC）从最初的血液涂片，证实肿瘤t细胞增殖与皮肤t细胞淋巴瘤一致，可能有短暂的白血病期。尽管进行了化疗，但病情的缓解是短暂的，患者病情复发，最终导致安乐死。本病例是一例罕见的短暂性白血病，可能起源于原发性皮肤t细胞淋巴瘤，强调需要综合血液学、生物化学、细胞学、流式细胞术和免疫分型进行诊断，以避免血液恶性肿瘤的错误分类。

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