Veterinary clinical pathology最新文献_第8页

Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma 犬白细胞诱导的低温沉淀、低温血浆和新鲜血浆止血活性的比较。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-16 DOI: 10.1111/vcp.70017

Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio

Background

To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).

Objectives

We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.

Methods

Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.

Results

FVIII activity was higher in LR-CRYO (p = 0.0001) versus LR-FP. vWF activity (p < 0.0001) and fibrinogen concentration (p = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (p < 0.0001) and LR-FP (p = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (p < 0.0001) and LR-CRYO (p < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (p = 0.0062) in refrigerated LR-CRYO.

Conclusions

Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.

背景：迄今为止，尚未有研究报道犬白细胞诱导低温沉淀（LR-CRYO）和白细胞诱导低温血浆（LR-CPP）的止血活性评估。目的：比较LR-CRYO和LR-CPP对白细胞诱导新鲜血浆（LR-FP）的止血活性，并评价冷冻和解冻后再冷冻对LR-CRYO的保存效果。方法：取10只供犬新鲜血液450 ml，经白细胞诱导，分离成LR-FP，冷冻（-20℃），得到白细胞诱导的新鲜冷冻血浆（LR-FFP）。LR-FFP进一步分离为LR-CRYO和LR-CPP。LR-CRYO冷冻、解冻后分成两袋，一袋冷藏24小时，一袋再冷冻7天。测定LR-FP、LR-CRYO、LR-CPP、冷藏和再冷冻LR-CRYO中凝血因子VIII （FVIII）和X （FX）活性、凝血酶原时间、活化部分凝血酶时间、抗凝血酶III （ATIII）活性（ATA）、总蛋白、白蛋白、纤维蛋白原和d -二聚体浓度以及血管性血友病因子（vWF）活性。结果：与LR-FP相比，LR-CRYO的FVIII活性更高（p = 0.0001）。结论：由于FVIII在LR-CRYO中的浓度高于LR-FP，因此当该因子缺乏时，LR-CRYO可能是一种成分治疗方法。由于再冷冻LR-CRYO与再冷冻LR-CRYO无统计学差异，因此解冻后可以再次冷冻LR-CRYO。

{"title":"Comparison of Hemostatic Activity in Canine Leukoreduced Cryoprecipitate, Cryopoor Plasma, and Fresh Plasma","authors":"Roberta Perego, Eva Spada, Luciana Baggiani, Giuliano Ravasio, Enrica Zucca, Graziella Vanosi, Giancarlo Ruffo, Daniela Proverbio","doi":"10.1111/vcp.70017","DOIUrl":"10.1111/vcp.70017","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>To date, no studies have reported the evaluation of hemostatic activity in canine leukoreduced cryoprecipitate (LR-CRYO) and leukoreduced cryopoor plasma (LR-CPP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>We aimed to compare the hemostatic activity of LR-CRYO and LR-CPP to leukoreduced fresh plasma (LR-FP) and to evaluate the preservation of LR-CRYO by refrigeration and refreezing after thawing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Four hundred fifty milliliters of fresh blood was collected from ten donor dogs, leukoreduced, and separated into LR-FP, then frozen (−20°C) to obtain leukoreduced fresh frozen plasma (LR-FFP). LR-FFP was further separated into LR-CRYO and LR-CPP. LR-CRYO was frozen, thawed, and divided into two bags, one refrigerated for 24 h and one refrozen for 7 days. Factor VIII (FVIII) and X (FX) activity, prothrombin time, activated partial thromboplastin time, antithrombin III (ATIII) activity (ATA), total protein, albumin, fibrinogen, and D-dimer concentration, and von Willebrand Factor (vWF) activity were measured in LR-FP, LR-CRYO, LR-CPP, refrigerated, and refrozen LR-CRYO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FVIII activity was higher in LR-CRYO (<i>p</i> = 0.0001) versus LR-FP. vWF activity (<i>p</i> < 0.0001) and fibrinogen concentration (<i>p</i> = 0.0012) were lower in LR-CPP versus LR-FP. FX activity was higher in LR-CPP (<i>p</i> < 0.0001) and LR-FP (<i>p</i> = 0.0002) versus LR-CRYO, and albumin concentration was higher in LR-CPP versus LR-FP (<i>p</i> < 0.0001) and LR-CRYO (<i>p</i> < 0.0001). No statistically significant difference was found in refrigerated or refrozen LR-CRYO as compared with LR-CRYO, excluding ATA, which was lower (<i>p</i> = 0.0062) in refrigerated LR-CRYO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Because the concentration of FVIII is higher in LR-CRYO than in LR-FP, LR-CRYO is a possible component therapy when this factor is deficient. Since no statistically significant difference was found in refrozen LR-CRYO as compared with LR-CRYO, LR-CRYO can be frozen after thawing for reuse.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"120-131"},"PeriodicalIF":1.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual Cytologic Findings in a Dog With a Keratinizing Cyst and Cutaneous Asthenia 犬角化囊肿和皮肤衰弱的异常细胞学表现。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-12 DOI: 10.1111/vcp.70019

Jeremy P. Bessett, Kimberley N. Sebastian

A 5-year-old spayed female golden retriever with a history of cutaneous asthenia (Ehlers-Danlos-like syndrome) was presented with a newly noted large hindlimb mass. Cytologic evaluation performed at the referring veterinarian was consistent with a keratinizing lesion. However, a second cytologic sample was obtained from the mass, aspirating from multiple aspects, and was concerning for a sarcoma. The mass was excised and submitted for histologic evaluation. The mass effect was diagnosed as a keratinizing cyst. Additionally, there were severe dermal collagen and elastin abnormalities with multifocal fibrosis and hemorrhage (consistent with cutaneous asthenia). To the best of the authors' knowledge, this is the first report of the cytologic findings in the skin of a canine with cutaneous asthenia.

一只5岁的雌性金毛猎犬，有皮肤衰弱史（埃勒斯-丹洛斯样综合征），出现了一个新发现的大后肢肿块。在转诊兽医处进行的细胞学评估与角化病变一致。然而，从肿块中获得第二个细胞学样本，从多个方面抽吸，并考虑为肉瘤。切除肿块并提交组织学评估。肿块效应诊断为角化性囊肿。此外，有严重的真皮胶原蛋白和弹性蛋白异常，伴有多灶性纤维化和出血（符合皮肤衰弱）。据作者所知，这是第一个报告的细胞学发现在皮肤的狗与皮肤衰弱。

引用次数: 0

What Is Your Diagnosis: Mediastinal Mass in a Dog 你的诊断是什么：狗的纵隔肿块。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-09 DOI: 10.1111/vcp.70028

Jelena Palić, Jaco Jan van der Lugt, Karin Troedson, Helena Nyman Lee

引用次数: 0

The Value of Neutrophil Cell Population Data Parameters as Markers of Systemic Inflammation in Dogs and Cats 中性粒细胞群体数据参数作为狗和猫全身炎症标志物的价值。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-06 DOI: 10.1111/vcp.70029

Dylan S. O′ Toole, Tim L. Williams, Cassia H. Z. Hare

Background

Neutrophil cell population data (CPD), including fluorescent light intensity (NE-SFL) and side scatter (NE-SSC), are promising inflammatory markers in human sepsis but remain unexplored in dogs and cats.

Objectives

Determine the diagnostic utility of NE-SSC and NE-SFL for detecting systemic inflammation in dogs and cats.

Methods

Dogs and cats with archived CPD, blood films, and acute phase protein (APP) measurements were included. Increased C-reactive protein (CRP) in dogs and Serum Amyloid A (SAA) in cats were considered indicative of systemic inflammation. CPD was compared with APPs, white cell count (WCC), neutrophil count, band neutrophil count, and toxic change grade. Optimal cut-offs and associated sensitivities and specificities were calculated using ROC curve analysis. Correlations were assessed using Spearman's coefficient.

Results

NE-SFL and NE-SSC were significantly increased in dogs and cats with systemic inflammation. The area under the curve (AUC) of NE-SFL was higher than that of NE-SSC, WCC, and band neutrophil count in both dogs (0.82) and cats (0.77). The optimal NE-SFL cut-off for detecting systemic inflammation was > 41.7 ch in dogs (sensitivity 80%; specificity 66%) and > 37.4 ch in cats (sensitivity 75%; specificity 67%). NE-SFL was positively correlated with APPs, WCC, neutrophil count, and band neutrophil count in both species. NE-SSC was positively correlated with APPs in both species and, in dogs, also with WCC, neutrophil count, and band neutrophil count.

Conclusion

CPD, particularly NE-SFL, is a promising marker of inflammation in dogs and cats and could be especially useful when APP quantification or blood smear examination are unavailable.

背景：中性粒细胞群数据（CPD），包括荧光光强度（NE-SFL）和侧散射（NE-SSC），是人类败血症中有希望的炎症标志物，但在狗和猫中仍未被探索。目的：确定NE-SSC和NE-SFL在犬猫全身性炎症检测中的诊断价值。方法：包括存档的CPD、血片和急性期蛋白（APP）测量的狗和猫。狗的c反应蛋白（CRP）和猫的血清淀粉样蛋白A （SAA）升高被认为是全系统炎症的指示。比较CPD与app、白细胞计数（WCC）、中性粒细胞计数、带中性粒细胞计数、毒性变化分级。使用ROC曲线分析计算最佳截止点和相关的灵敏度和特异性。使用Spearman系数评估相关性。结果：NE-SFL和NE-SSC在全身性炎症的狗和猫中显著升高。NE-SFL的曲线下面积（AUC）在狗（0.82）和猫（0.77）中均高于NE-SSC、WCC和带状中性粒细胞计数。在犬中，NE-SFL检测全身性炎症的最佳临界值为41.7 ch(敏感性80%；特异性66%)和> 37.4 ch(敏感性75%；特异性67%)。NE-SFL与两种动物的APPs、WCC、中性粒细胞计数和带状中性粒细胞计数呈正相关。在两种动物中，NE-SSC与APPs呈正相关，在狗中，也与WCC、中性粒细胞计数和带状中性粒细胞计数呈正相关。结论：CPD，特别是NE-SFL，是一种很有希望的狗和猫炎症标志物，在APP量化或血液涂片检查不可用时特别有用。

{"title":"The Value of Neutrophil Cell Population Data Parameters as Markers of Systemic Inflammation in Dogs and Cats","authors":"Dylan S. O′ Toole, Tim L. Williams, Cassia H. Z. Hare","doi":"10.1111/vcp.70029","DOIUrl":"10.1111/vcp.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Neutrophil cell population data (CPD), including fluorescent light intensity (NE-SFL) and side scatter (NE-SSC), are promising inflammatory markers in human sepsis but remain unexplored in dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Determine the diagnostic utility of NE-SSC and NE-SFL for detecting systemic inflammation in dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Dogs and cats with archived CPD, blood films, and acute phase protein (APP) measurements were included. Increased C-reactive protein (CRP) in dogs and Serum Amyloid A (SAA) in cats were considered indicative of systemic inflammation. CPD was compared with APPs, white cell count (WCC), neutrophil count, band neutrophil count, and toxic change grade. Optimal cut-offs and associated sensitivities and specificities were calculated using ROC curve analysis. Correlations were assessed using Spearman's coefficient.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NE-SFL and NE-SSC were significantly increased in dogs and cats with systemic inflammation. The area under the curve (AUC) of NE-SFL was higher than that of NE-SSC, WCC, and band neutrophil count in both dogs (0.82) and cats (0.77). The optimal NE-SFL cut-off for detecting systemic inflammation was > 41.7 ch in dogs (sensitivity 80%; specificity 66%) and > 37.4 ch in cats (sensitivity 75%; specificity 67%). NE-SFL was positively correlated with APPs, WCC, neutrophil count, and band neutrophil count in both species. NE-SSC was positively correlated with APPs in both species and, in dogs, also with WCC, neutrophil count, and band neutrophil count.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CPD, particularly NE-SFL, is a promising marker of inflammation in dogs and cats and could be especially useful when APP quantification or blood smear examination are unavailable.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"78-86"},"PeriodicalIF":1.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Point-of-Care Cardiac Troponin I Immunoassays Have Acceptable Analytical Performance for the Detection of Measurands of Cardiac Troponin I Cardiac Muscle Homogenates From Southern-Central Black Rhinoceros (Diceros bicornis minor) and Southern White Rhinoceros (Ceratotherium simum simum) 两种护理点心肌肌钙蛋白I免疫测定法对中南部黑犀牛（Diceros bicornis minor）和南部白犀牛（Ceratotherium simum simum）的心肌肌钙蛋白I匀浆的检测具有可接受的分析性能。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-04 DOI: 10.1111/vcp.70016

Yolandi Rautenbach, Leith C. R. Meyer, Amelia Goddard, Peter E. Buss, Emma H. Hooijberg

Background

Skeletal and possible cardiac muscle damage has been reported in chemically immobilized and transported African rhinoceros during conservation-related activities. The extent of cardiac muscle injury in these rhinoceros is unknown due to a lack of validated cardiac troponin I (cTnI) assays. However, recently, five human cTnI assays were deemed suitable for analytical validation in African rhinoceros based on cTnI sequencing results.

Objectives

The first objective was to validate two cTnI immunoassay point-of-care analyzers (POCAs) in African rhinoceros and, secondly, to perform quality control (QC) validation for the POCAs.

Methods

Analytical validation of the Stratus CS Acute Care Troponin I cTnI immunoassay and Atellica VTLi high sensitivity cTnI (hs-cTnI) assay was performed using rhinoceros serum samples and species-specific cardiac muscle homogenate. Experiments included precision studies, reportable range, hemoglobin interference studies, recovery studies, and detection limit studies, with results assessed against prescribed total allowable error (TE_a) performance goals. Commercial quality control material (QCM) data were used to calculate bias and imprecision for QC validation.

Results

Imprecision was acceptable (1.9%–10.3%) and met low cTnI concentration performance goals. Reportable ranges were similar to the manufacturer's specifications. High hemoglobin concentrations in white rhinoceros resulted in a positive bias in the Stratus CS. A simple 1_3s QC rule using two levels of QCM and a TEa of 70% could be used in both analyzers, except at very low cTnI concentrations in the Atellica VTLi.

Conclusions

Both cTnI POCAs are suitable for use in African rhinoceros, and analytical performance goals for low cTnI concentrations in hs-cTnI assays were met.

背景：在与保护有关的活动中，化学固定和运输的非洲犀牛已经报道了骨骼和可能的心肌损伤。由于缺乏有效的心肌肌钙蛋白I （cTnI）检测，这些犀牛的心肌损伤程度尚不清楚。然而，最近，基于cTnI测序结果，五种人类cTnI测定被认为适合于非洲犀牛的分析验证。目的：第一个目的是验证非洲犀牛的两种cTnI免疫测定护理点分析仪（poca），其次，对poca进行质量控制（QC）验证。方法：采用犀牛血清样品和种特异性心肌匀浆，对Stratus CS急性护理肌钙蛋白I cTnI免疫测定法和Atellica VTLi高灵敏度cTnI （hs-cTnI）测定法进行分析验证。实验包括精密度研究、报告范围、血红蛋白干扰研究、回收率研究和检出限研究，并根据规定的总允许误差（TEa）性能目标对结果进行评估。商业质量控制材料（QCM）数据用于计算偏差和不精度，以进行QC验证。结果：不精确性可接受（1.9% ~ 10.3%），满足低cTnI浓度性能目标。可报告范围与制造商的规格相似。白犀牛血红蛋白浓度高导致Stratus CS阳性偏倚。除了Atellica VTLi中非常低的cTnI浓度外，使用两级QCM和70% TEa的简单13s QC规则可用于两种分析仪。结论：两种cTnI poca均适用于非洲犀牛，且在hs-cTnI低浓度检测中均满足分析性能目标。

{"title":"Two Point-of-Care Cardiac Troponin I Immunoassays Have Acceptable Analytical Performance for the Detection of Measurands of Cardiac Troponin I Cardiac Muscle Homogenates From Southern-Central Black Rhinoceros (Diceros bicornis minor) and Southern White Rhinoceros (Ceratotherium simum simum)","authors":"Yolandi Rautenbach, Leith C. R. Meyer, Amelia Goddard, Peter E. Buss, Emma H. Hooijberg","doi":"10.1111/vcp.70016","DOIUrl":"10.1111/vcp.70016","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Skeletal and possible cardiac muscle damage has been reported in chemically immobilized and transported African rhinoceros during conservation-related activities. The extent of cardiac muscle injury in these rhinoceros is unknown due to a lack of validated cardiac troponin I (cTnI) assays. However, recently, five human cTnI assays were deemed suitable for analytical validation in African rhinoceros based on cTnI sequencing results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The first objective was to validate two cTnI immunoassay point-of-care analyzers (POCAs) in African rhinoceros and, secondly, to perform quality control (QC) validation for the POCAs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Analytical validation of the Stratus CS Acute Care Troponin I cTnI immunoassay and Atellica VTLi high sensitivity cTnI (hs-cTnI) assay was performed using rhinoceros serum samples and species-specific cardiac muscle homogenate. Experiments included precision studies, reportable range, hemoglobin interference studies, recovery studies, and detection limit studies, with results assessed against prescribed total allowable error (TE<sub>a</sub>) performance goals. Commercial quality control material (QCM) data were used to calculate bias and imprecision for QC validation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Imprecision was acceptable (1.9%–10.3%) and met low cTnI concentration performance goals. Reportable ranges were similar to the manufacturer's specifications. High hemoglobin concentrations in white rhinoceros resulted in a positive bias in the Stratus CS. A simple 1<sub>3s</sub> QC rule using two levels of QCM and a TEa of 70% could be used in both analyzers, except at very low cTnI concentrations in the Atellica VTLi.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both cTnI POCAs are suitable for use in African rhinoceros, and analytical performance goals for low cTnI concentrations in hs-cTnI assays were met.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"142-153"},"PeriodicalIF":1.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Is Your Diagnosis? Blood Smear From a Kitten 你的诊断是什么？小猫的血涂片。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-04 DOI: 10.1111/vcp.70011

Erin E. Farrell, Renata M. Mammone, Gayle C. Johnson, Ji-Hey Lim, Margret L. Casal, Angela B. Royal

引用次数: 0

Veterinary Laboratory Quality, Basic Principles and Selected Topics, 1st Edition. By Kathleen Freeman, Stefanie Klenner-Gastreich, and Jeremie Korchia, Madison, WI, USA: Westgard QC, 2024. 376 pp. $80 (paperback). ISBN: 978-1-886958-39-5 兽医实验室质量，基本原则和选题，第1版。作者：Kathleen Freeman， Stefanie Klenner-Gastreich和Jeremie Korchia， Madison， WI, USA: Westgard QC, 2024。376页，平装本80美元。ISBN: 978-1-886958-39-5

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-04 DOI: 10.1111/vcp.70010

Simone Manzocchi

引用次数: 0

What's Your Diagnosis? Dog With a Laryngeal Mass 你的诊断是什么？有喉部肿块的狗。

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-04 DOI: 10.1111/vcp.70014

Benjamin J. Haythornthwaite, Dylan Yaffy, Emma J. Holmes

An 8-year, 3-month-old female, entire Boxer dog presented to the Queen Mother Hospital for Animals (QMHA), Royal Veterinary College (RVC) for evaluation of increased upper airway noise and dysphagia. A laryngeal mass had been identified by the referring veterinarian 5 months previously while the dog was undergoing general anesthetic for removal of oral masses (confirmed as peripheral odontogenic fibromas). At this time, no clinical signs were associated with the mass. However, in the weeks prior to presentation, the dog became unable to tolerate solid food and started showing respiratory symptoms relating to the mass and was subsequently referred for evaluation. CT examination revealed a 35 × 28 × 39 mm soft tissue mass occupying the right side of the rima glottidis, displacing the laryngeal lumen. The mass extended from the level of the rostral margin of the tympanic bulla to the level of C1 and dorsally into the caudal aspect of the nasopharynx. Direct aspirate preparations (Figures 1 and 2) from the laryngeal mass were air-dried, fixed, and stained using modified Wright stain (Hema-tek 3000, Siemens).

Incisional biopsy samples obtained at the same time as the aspirates were fixed in formalin, routinely processed, and stained with H&E.

Sections had a densely cellular pleomorphic malignant neoplasm, composed of diffuse sheets of irregularly polygonal to elongate cells of two populations. The more prominent population had large amounts of vacuolated cytoplasm with lower numbers of smaller round cells with moderate amounts of finely granular eosinophilic cytoplasm. Most cells had a single irregularly round nucleus with one prominent large nucleolus. Anisocytosis and anisokaryosis of the larger cells were marked, with frequent multinucleated giant cells and 57 bizarre mitotic figures in 2.37 mm² (Figure 3). Atypia of the smaller cell population was mild. The histological interpretation was an atypical malignant neoplasm with differentials including rhabdomyoma/sarcoma, oncocytoma, granular cell tumor, amelanotic melanoma, or histiocytic sarcoma.

Histochemical staining revealed strong cytoplasmic positivity for periodic acid-Schiff (PAS) in the small cells but negativity in the larger cells, diffuse strong membranous, and cytoplasmic positivity for vimentin in approximately 95% of cells (Figure 4), diffuse moderate cytoplasmic positivity for desmin in approximately 80% of cells, and negativity for toluidine blue, phosphotungstic acid hematoxylin (PTAH), cytokeratin, ionized calcium-binding adaptor molecule 1 (Iba-1), melan-A, smooth muscle actin (α-sma), neuron-specific enolase (NSE), and S100 (Table 1).

Based on these staining characteristics and cellular pleomorphism, a diagnosis of rhabdomyosarcoma was made.

Laryngeal rhabdomyosarcoma is mainly reported in adult dogs, being locally invasive but rarely metastatic. This local invasion makes complete excision difficult, with tumor recurrence often resu

一只8岁3个月大的雌性整只拳师犬被送到皇家兽医学院（RVC）动物太后医院（QMHA）评估上呼吸道噪音增加和吞咽困难。5个月前，当狗接受全身麻醉以去除口腔肿块（证实为周围牙源性纤维瘤）时，转诊兽医发现喉肿块。此时，没有与肿块相关的临床体征。然而，在发病前几周，该犬无法耐受固体食物，并开始出现与肿块有关的呼吸道症状，随后被转诊进行评估。CT检查发现声门边缘右侧有一35 × 28 × 39 mm软组织肿块，取代喉腔。肿块从鼓室球吻侧缘水平延伸至C1水平，并向背侧延伸至鼻咽尾侧。从喉部肿块中直接抽吸的制剂（图1和2）被风干、固定，并使用改良的Wright染色剂（Hema-tek 3000, Siemens）进行染色。与抽吸器同时获得的切口活检样本在福尔马林中固定，常规处理，并用H&amp；E染色。切片有一个密集的细胞多形性恶性肿瘤，由不规则多边形的扩散片到两个种群的细长细胞组成。更突出的群体有大量的液泡细胞质，少量的小圆形细胞和适量的细颗粒性嗜酸性细胞质。大多数细胞有一个不规则的圆形细胞核和一个突出的大核仁。在2.37 mm2内可见多核巨细胞和57个奇异的有丝分裂象（图3）。小细胞群的异型性较轻。组织学解释为非典型恶性肿瘤，可鉴别为横纹肌瘤/肉瘤、嗜瘤细胞瘤、颗粒细胞瘤、无色素黑色素瘤或组织细胞肉瘤。组织化学染色显示，在小细胞中周期性酸-希夫（PAS）呈强阳性，而在大细胞中呈阴性，约95%的细胞弥漫强膜质和波形蛋白呈细胞质阳性（图4），约80%的细胞弥漫中等程度的desmin阳性，甲苯胺蓝、磷tungstic acid hematoxylin （PTAH）、细胞角蛋白、离子钙结合接头分子1 （Iba-1）、黑色素- a、平滑肌肌动蛋白（α-sma）、神经元特异性烯醇化酶（NSE）和S100（表1）。根据这些染色特征和细胞多形性，诊断为横纹肌肉瘤。喉部横纹肌肉瘤主要发生于成年犬，具有局部侵袭性，但很少发生转移。这种局部侵袭使得完全切除变得困难，肿瘤复发往往导致安乐死[1,2]。如本病例所示，如果没有免疫组化（IHC）染色特征，由于细胞多形性或存在多个不同的细胞群，诊断可能会很困难[3,4]。细胞形态的范围被怀疑类似于肌细胞的各种发育表型，从圆形的未成熟的成肌细胞和不同数量的粉红色细胞质到更成熟的多核肌小管细胞[1]。然而，大的、高颗粒到液泡状的细胞通常表现出明显的细胞多形性，这使得细胞学诊断非常具有挑战性。横纹肌肉瘤在组织学上可分为四个亚类，包括肺泡型、葡萄样型、胚胎型和罕见的多形性横纹肌肉瘤，从而可以预测其生物学行为。肺泡RMS进一步分为经典型和实型，胚胎型分为横纹肌母细胞型（以圆形至多角形细胞为主，胞浆呈粉红色变化）、肌管型（以多核PTAH阳性“带状细胞”为主）和纺锤型（在兽用物种中罕见）[1,3]。IHC的特征往往是可变的，这取决于细胞分化的程度和肌生成调节因子表达的可变性。在成肌细胞阶段，波形蛋白是第一个表达的中间丝，随着进一步分化，波形蛋白表达。即使在未分化和高度多形性的RMS中，Desmin也是有用的。该标志物的表达同时缺乏细胞角蛋白和PAS的表达将排除癌细胞瘤[5]的诊断。理想情况下，为了进一步确认骨骼肌的起源，可以考虑MyoD1和myogenin的表达，代表早期胚胎转录因子，尽管表达可能取决于分化程度[1]。不幸的是，我们的机构没有这些标记。平滑肌肌动蛋白（SMA）阴性染色排除平滑肌来源。尽管高颗粒形态和PAS阳性，但基于S100和NSE阴性[5]排除颗粒细胞肿瘤。组织细胞肉瘤（Iba-1阴性）、无色素黑色素瘤（黑色素a和S100阴性）或间变性癌（细胞角蛋白阴性）的研究结果也不一致。本病例最终表现为一种罕见的肿瘤，鉴于细胞多形性的程度，这为诊断提供了挑战，最终需要免疫组织化学进行诊断。作者声明无利益冲突。

{"title":"What's Your Diagnosis? Dog With a Laryngeal Mass","authors":"Benjamin J. Haythornthwaite, Dylan Yaffy, Emma J. Holmes","doi":"10.1111/vcp.70014","DOIUrl":"10.1111/vcp.70014","url":null,"abstract":"<p>An 8-year, 3-month-old female, entire Boxer dog presented to the Queen Mother Hospital for Animals (QMHA), Royal Veterinary College (RVC) for evaluation of increased upper airway noise and dysphagia. A laryngeal mass had been identified by the referring veterinarian 5 months previously while the dog was undergoing general anesthetic for removal of oral masses (confirmed as peripheral odontogenic fibromas). At this time, no clinical signs were associated with the mass. However, in the weeks prior to presentation, the dog became unable to tolerate solid food and started showing respiratory symptoms relating to the mass and was subsequently referred for evaluation. CT examination revealed a 35 × 28 × 39 mm soft tissue mass occupying the right side of the rima glottidis, displacing the laryngeal lumen. The mass extended from the level of the rostral margin of the tympanic bulla to the level of C1 and dorsally into the caudal aspect of the nasopharynx. Direct aspirate preparations (Figures 1 and 2) from the laryngeal mass were air-dried, fixed, and stained using modified Wright stain (Hema-tek 3000, Siemens).</p><p>Incisional biopsy samples obtained at the same time as the aspirates were fixed in formalin, routinely processed, and stained with H&E.</p><p>Sections had a densely cellular pleomorphic malignant neoplasm, composed of diffuse sheets of irregularly polygonal to elongate cells of two populations. The more prominent population had large amounts of vacuolated cytoplasm with lower numbers of smaller round cells with moderate amounts of finely granular eosinophilic cytoplasm. Most cells had a single irregularly round nucleus with one prominent large nucleolus. Anisocytosis and anisokaryosis of the larger cells were marked, with frequent multinucleated giant cells and 57 bizarre mitotic figures in 2.37 mm<sup>2</sup> (Figure 3). Atypia of the smaller cell population was mild. The histological interpretation was an atypical malignant neoplasm with differentials including rhabdomyoma/sarcoma, oncocytoma, granular cell tumor, amelanotic melanoma, or histiocytic sarcoma.</p><p>Histochemical staining revealed strong cytoplasmic positivity for periodic acid-Schiff (PAS) in the small cells but negativity in the larger cells, diffuse strong membranous, and cytoplasmic positivity for vimentin in approximately 95% of cells (Figure 4), diffuse moderate cytoplasmic positivity for desmin in approximately 80% of cells, and negativity for toluidine blue, phosphotungstic acid hematoxylin (PTAH), cytokeratin, ionized calcium-binding adaptor molecule 1 (Iba-1), melan-A, smooth muscle actin (α-sma), neuron-specific enolase (NSE), and S100 (Table 1).</p><p>Based on these staining characteristics and cellular pleomorphism, a diagnosis of rhabdomyosarcoma was made.</p><p>Laryngeal rhabdomyosarcoma is mainly reported in adult dogs, being locally invasive but rarely metastatic. This local invasion makes complete excision difficult, with tumor recurrence often resu","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 S1","pages":"S41-S44"},"PeriodicalIF":1.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the influence of hepatic triglyceride and lipoprotein lipases on lipase assays used to diagnose pancreatitis in dogs and cats 评估肝甘油三酯和脂蛋白脂肪酶对用于诊断狗和猫胰腺炎的脂肪酶测定的影响。

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-03 DOI: 10.1111/vcp.70002

Hiroki Harada, Masahiro Tamura, Kumi Ishii, Tatsuyuki Osuga, Hiroshi Ohta

Background

Pancreas-specific lipase and lipase activity measured by immunological assays (Spec cPL and Spec fPL) and colorimetric assays (1,2-o-dilauryl-rac-glycelo-3-glutaric acid-(6'-methylresorufin) ester [DGGR] and triolein), respectively, are used to diagnose pancreatitis in both dogs and cats. However, DGGR and triolein assays may be influenced by extrapancreatic lipases, including hepatic triglyceride lipase (HTGL) and lipoprotein lipase (LPL).

Objectives

To investigate the effect of extrapancreatic lipases on immunological and colorimetric assays by measuring changes in HTGL and LPL activity following heparin administration.

Methods

Six healthy Beagles and six adult purpose-bred cats were enrolled. HTGL and LPL activities were induced by intravenous heparin administration. Serum samples were collected at baseline and at 5-, 10-, 15-, and 60-min following heparin injection. Spec cPL, Spec fPL, and lipase activities were measured using DGGR and triolein assays, whereas HTGL and LPL activities were measured using their respective assays.

Results

Spec cPL and Spec fPL levels showed no significant changes following heparin administration. Conversely, DGGR-based and triolein-based lipase activities, as well as HTGL and LPL activities, were significantly increased after heparin administration in both dogs and cats. HTGL and LPL activities showed significant positive correlations with DGGR-based (P < .001, r = .90 for both) and triolein-based (P < .001, r = .63 and P < .001, r = .68, respectively) lipase activities, but not with Spec cPL and Spec fPL.

Conclusions

DGGR- and triolein-based lipase activities are influenced by HTGL and LPL activities, as their substrates are hydrolyzed by pancreatic lipase, HTGL, and LPL.

背景：胰腺特异性脂肪酶和脂肪酶活性分别通过免疫测定法（Spec cPL和Spec fPL）和比色测定法（1,2-o-二萘酰基-丙基-甘油-3-戊二酸-（6'-甲基间苯二酚）酯[DGGR]和三油酸）测定，用于诊断狗和猫的胰腺炎。然而，DGGR和三油酸测定可能受到胰腺外脂肪酶的影响，包括肝甘油三酯脂肪酶（HTGL）和脂蛋白脂肪酶（LPL）。目的：通过测定肝素给药后HTGL和LPL活性的变化，探讨胰外脂肪酶对免疫和比色测定的影响。方法：选取6只健康的比格犬和6只成年专用猫。肝素对HTGL和LPL活性的影响。在基线和注射肝素后5、10、15和60分钟采集血清样本。采用DGGR和三油酸测定法测定Spec cPL、Spec fPL和脂肪酶活性，而HTGL和LPL活性分别采用各自的测定法测定。结果：肝素给药后，Spec cPL和Spec fPL水平无明显变化。相反，在给药后，狗和猫的dggr和triolein脂肪酶活性以及HTGL和LPL活性均显著增加。HTGL和LPL活性与基于DGGR的脂肪酶活性呈显著正相关(P)。结论：基于DGGR和三油醇的脂肪酶活性受HTGL和LPL活性的影响，因为它们的底物被胰脂肪酶、HTGL和LPL水解。

{"title":"Evaluating the influence of hepatic triglyceride and lipoprotein lipases on lipase assays used to diagnose pancreatitis in dogs and cats","authors":"Hiroki Harada, Masahiro Tamura, Kumi Ishii, Tatsuyuki Osuga, Hiroshi Ohta","doi":"10.1111/vcp.70002","DOIUrl":"10.1111/vcp.70002","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Pancreas-specific lipase and lipase activity measured by immunological assays (Spec cPL and Spec fPL) and colorimetric assays (1,2-<i>o</i>-dilauryl-<i>rac</i>-glycelo-3-glutaric acid-(6'-methylresorufin) ester [DGGR] and triolein), respectively, are used to diagnose pancreatitis in both dogs and cats. However, DGGR and triolein assays may be influenced by extrapancreatic lipases, including hepatic triglyceride lipase (HTGL) and lipoprotein lipase (LPL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To investigate the effect of extrapancreatic lipases on immunological and colorimetric assays by measuring changes in HTGL and LPL activity following heparin administration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Six healthy Beagles and six adult purpose-bred cats were enrolled. HTGL and LPL activities were induced by intravenous heparin administration. Serum samples were collected at baseline and at 5-, 10-, 15-, and 60-min following heparin injection. Spec cPL, Spec fPL, and lipase activities were measured using DGGR and triolein assays, whereas HTGL and LPL activities were measured using their respective assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Spec cPL and Spec fPL levels showed no significant changes following heparin administration. Conversely, DGGR-based and triolein-based lipase activities, as well as HTGL and LPL activities, were significantly increased after heparin administration in both dogs and cats. HTGL and LPL activities showed significant positive correlations with DGGR-based (<i>P</i> < .001, <i>r</i> = .90 for both) and triolein-based (<i>P</i> < .001, <i>r</i> = .63 and <i>P</i> < .001, <i>r</i> = .68, respectively) lipase activities, but not with Spec cPL and Spec fPL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>DGGR- and triolein-based lipase activities are influenced by HTGL and LPL activities, as their substrates are hydrolyzed by pancreatic lipase, HTGL, and LPL.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"132-141"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of blood erythroid parameters in male broiler chickens (Ross 308) with the Sysmex XT-2000iV and Sysmex XN-1000V analyzers and determination of hematological reference intervals obtained with manual and instrumental methods 用Sysmex XT-2000iV和Sysmex XN-1000V分析仪评价雄性肉鸡（Ross 308）的红细胞参数，并测定手工和仪器方法获得的血液学参考区间

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology

Pub Date : 2025-06-03 DOI: 10.1111/vcp.70009

Damien Legroux, Laura Kersten, Gaelle Barral, Alexane Mauras, Thierry Buronfosse, Eve Ramery

Background

The presence of both nucleated RBC and thrombocytes in birds makes the determination of hematological variables difficult. This leads to time-consuming manual evaluation. New-generation analyzers are hypothesized to be both time-saving and accurate.

Objectives

The objective was to evaluate the performance of two hematology analyzers in 27-day-old broilers and to establish reference intervals (RIs) for RBC and WBC variables obtained by automated and manual methods.

Methods

Automated HCT, WBC count, and differentials obtained using Sysmex XT-2000iV and XN-1000V on 309 and 56 EDTA blood samples from Ross 308 male broilers, respectively, were compared to manual PCV, manual WBC count, and differentials from November 2021 to November 2022. RIs for automated HCT and RBC variables (from impedance channel) on one hand, and manual PCV and WBC's variables on the other hand, were established.

Results

The Sysmex XT-2000iV was accurate for HCT and outperformed the XN-1000V but, both automated methods and manual methods cannot be considered interchangeable. Constant error, proportional error, and bias were identified and calculated for each analyzer for HCT. Both analyzers were unable to enumerate WBC. RIs were established as follows: HCT-XT (L/L) [0.27–0.36]; HCT-XN (L/L) [0.24–0.38]; manual PCV (L/L) [0.27–0.37]; manual WBC (×10⁹/L) [10.0–38.4]; manual differential (×10⁹/L) with heterophils [1.5–16.2]; lymphocytes [5.1–18.9]; monocytes [0.0–2.1]; eosinophils [0.0–2.2]; basophils [0.0–3.1].

Conclusions

Both analyzers are usable for the evaluation of HCT in Ross 308 chickens and in specific conditions for the other RBC variables. Manual methods are still preferred for the evaluation of WBC variables.

背景：鸟类中有核红细胞和血小板的存在使得血液学变量的测定变得困难。这将导致耗时的手动评估。新一代分析仪被认为既节省时间又准确。目的：目的是评估两种血液分析仪在27日龄肉鸡中的性能，并建立自动和手动方法获得的RBC和WBC变量的参考区间（RIs）。方法：采用Sysmex XT-2000iV和XN-1000V分别对罗斯308雄性肉鸡的309份和56份EDTA血液样本进行自动HCT、白细胞计数和差异检测，并与人工PCV、人工白细胞计数和差异检测进行比较。建立了自动HCT和RBC变量（阻抗通道）的RIs，以及手动PCV和WBC变量的RIs。结果：Sysmex XT-2000iV对HCT的准确性优于XN-1000V，但自动方法和手动方法不能互换。确定并计算每个HCT分析仪的恒定误差、比例误差和偏差。两种分析仪都不能枚举WBC。HCT-XT (L/L) [0.27-0.36]；Hct-xn (l / l) [0.24-0.38]；手动PCV (L/L) [0.27-0.37]；手动白细胞（×109/L） [10.0-38.4]；手动差速器（×109/L）与异亲[1.5-16.2]；淋巴细胞(5.1 - -18.9);单核细胞(0.0 - -2.1);嗜酸性粒细胞(0.0 - -2.2);嗜碱粒细胞(0.0 - -3.1)。结论：这两种分析仪都可用于评估罗斯308鸡的HCT，并在特定条件下评估其他RBC变量。手动方法仍然是优选的WBC变量的评估。

{"title":"Evaluation of blood erythroid parameters in male broiler chickens (Ross 308) with the Sysmex XT-2000iV and Sysmex XN-1000V analyzers and determination of hematological reference intervals obtained with manual and instrumental methods","authors":"Damien Legroux, Laura Kersten, Gaelle Barral, Alexane Mauras, Thierry Buronfosse, Eve Ramery","doi":"10.1111/vcp.70009","DOIUrl":"10.1111/vcp.70009","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The presence of both nucleated RBC and thrombocytes in birds makes the determination of hematological variables difficult. This leads to time-consuming manual evaluation. New-generation analyzers are hypothesized to be both time-saving and accurate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The objective was to evaluate the performance of two hematology analyzers in 27-day-old broilers and to establish reference intervals (RIs) for RBC and WBC variables obtained by automated and manual methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Automated HCT, WBC count, and differentials obtained using Sysmex XT-2000iV and XN-1000V on 309 and 56 EDTA blood samples from Ross 308 male broilers, respectively, were compared to manual PCV, manual WBC count, and differentials from November 2021 to November 2022. RIs for automated HCT and RBC variables (from impedance channel) on one hand, and manual PCV and WBC's variables on the other hand, were established.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The Sysmex XT-2000iV was accurate for HCT and outperformed the XN-1000V but, both automated methods and manual methods cannot be considered interchangeable. Constant error, proportional error, and bias were identified and calculated for each analyzer for HCT. Both analyzers were unable to enumerate WBC. RIs were established as follows: HCT-XT (L/L) [0.27–0.36]; HCT-XN (L/L) [0.24–0.38]; manual PCV (L/L) [0.27–0.37]; manual WBC (×10<sup>9</sup>/L) [10.0–38.4]; manual differential (×10<sup>9</sup>/L) with heterophils [1.5–16.2]; lymphocytes [5.1–18.9]; monocytes [0.0–2.1]; eosinophils [0.0–2.2]; basophils [0.0–3.1].</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both analyzers are usable for the evaluation of HCT in Ross 308 chickens and in specific conditions for the other RBC variables. Manual methods are still preferred for the evaluation of WBC variables.</p>\u0000 </section>\u0000 </div>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"54 2","pages":"106-119"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0