Pub Date : 2008-08-01DOI: 10.1017/S1479236208002192
Zhang Chi, Xie Conghua, Song Botao, L. Xun, L. Jun
{"title":"RNAi effects on regulation of endogenous acid invertase activity in potato ( Solanum tuberosum L.) tubers","authors":"Zhang Chi, Xie Conghua, Song Botao, L. Xun, L. Jun","doi":"10.1017/S1479236208002192","DOIUrl":"https://doi.org/10.1017/S1479236208002192","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116109363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002167
Cheng-jun Yang, W. Jun, Li-qiang Mu, Shao-Chen Li, GuanJun Liu, Chang-Qun Hu
A total of 791 microsatellites (SSRs) were isolated from 7055 Panax ginseng expressed sequence tags (ESTs). According to primer design criteria, 68 primer pairs for EST-SSR were designed. Under an appropriate polymerase chain reaction (PCR) system, all EST-SSR primer pairs were screened against genomic DNA of Ji'anchangbo and Fusong'ermaya from Panax ginseng , and 43 EST-SSR primer pairs out of the above 68 resulted in PCR products. Then, all 43 pairs were detected in nine P. ginseng , two Panax quinquefolius and two Acanthopanax senticosus cultivars for polymorphisms, and 26 pairs (60.47%) were found to be polymorphic, accounting for 38.23% of the total number of designed primer pairs. These results demonstrate the possibility of developing EST-SSR markers using P. ginseng ESTs.
{"title":"Development of an EST-SSR marker in Panax ginseng","authors":"Cheng-jun Yang, W. Jun, Li-qiang Mu, Shao-Chen Li, GuanJun Liu, Chang-Qun Hu","doi":"10.1017/S1479236208002167","DOIUrl":"https://doi.org/10.1017/S1479236208002167","url":null,"abstract":"A total of 791 microsatellites (SSRs) were isolated from 7055 Panax ginseng expressed sequence tags (ESTs). According to primer design criteria, 68 primer pairs for EST-SSR were designed. Under an appropriate polymerase chain reaction (PCR) system, all EST-SSR primer pairs were screened against genomic DNA of Ji'anchangbo and Fusong'ermaya from Panax ginseng , and 43 EST-SSR primer pairs out of the above 68 resulted in PCR products. Then, all 43 pairs were detected in nine P. ginseng , two Panax quinquefolius and two Acanthopanax senticosus cultivars for polymorphisms, and 26 pairs (60.47%) were found to be polymorphic, accounting for 38.23% of the total number of designed primer pairs. These results demonstrate the possibility of developing EST-SSR markers using P. ginseng ESTs.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"4 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131604239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002143
Wang Gong-jin, Tan Xiao-dong, Zhou Xiao-long, Xu Xiao-bo, F. Bi-qin
The developmental functions of oocytes of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) recombined with the nuclei of first polar bodies (Pbs I) were explored. Cumulus oocyte complexes (COCs) from the mice were collected after superovulation, then Pbs I were obtained from the COCs by 2% pronase treatment. The survival of Pbs I under different temperatures was identified by morphology and trypan blue staining. Later, the polar body I (Pb 1) nucleus and a little cytoplasm was injected into each oocyte, the nuclei of which had been enucleated by micromanipulation. Oocytes recombined with Pbs I were fertilized, then cultured in vitro in order to observe their further development. The results showed that the vigour of Pbs I was maintained for 12–14 h after superovulation, and was still maintained after 48 h at 4 °C. A total of 13 out of 117 recombined oocytes from Kunming and ICR mice, as well as 3 out of 38 recombined oocytes from C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice, developed into two-cell embryos. The experiments confirmed that mouse oocytes recombined with the nuclei of Pbs I could maintain fertilization and development. These results present valuable references for further utilization of genetic resources for farm animals
{"title":"In vitro fertilization and cleavage of mouse oocytes recombined with the first polar body","authors":"Wang Gong-jin, Tan Xiao-dong, Zhou Xiao-long, Xu Xiao-bo, F. Bi-qin","doi":"10.1017/S1479236208002143","DOIUrl":"https://doi.org/10.1017/S1479236208002143","url":null,"abstract":"The developmental functions of oocytes of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) recombined with the nuclei of first polar bodies (Pbs I) were explored. Cumulus oocyte complexes (COCs) from the mice were collected after superovulation, then Pbs I were obtained from the COCs by 2% pronase treatment. The survival of Pbs I under different temperatures was identified by morphology and trypan blue staining. Later, the polar body I (Pb 1) nucleus and a little cytoplasm was injected into each oocyte, the nuclei of which had been enucleated by micromanipulation. Oocytes recombined with Pbs I were fertilized, then cultured in vitro in order to observe their further development. The results showed that the vigour of Pbs I was maintained for 12–14 h after superovulation, and was still maintained after 48 h at 4 °C. A total of 13 out of 117 recombined oocytes from Kunming and ICR mice, as well as 3 out of 38 recombined oocytes from C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice, developed into two-cell embryos. The experiments confirmed that mouse oocytes recombined with the nuclei of Pbs I could maintain fertilization and development. These results present valuable references for further utilization of genetic resources for farm animals","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124456444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S147923620800212X
Z. Xiaohui, Ye Zhibiao, Zhang Yuyang, Hou Zheng, Li Hanxia
Recent advances and limitations of using zinc finger nuclease to stimulate a high efficiency of homologous recombination, and chimeric oligonucleotides to promote single base replacement in functional genomic research and plant genetic breeding, are systematically reviewed. Approaches to improve gene targeting efficiency through molecular modification of key pathways in plant homologous recombination are also discussed.
{"title":"Plant gene targeting and gene replacement: application to crop genetic improvement","authors":"Z. Xiaohui, Ye Zhibiao, Zhang Yuyang, Hou Zheng, Li Hanxia","doi":"10.1017/S147923620800212X","DOIUrl":"https://doi.org/10.1017/S147923620800212X","url":null,"abstract":"Recent advances and limitations of using zinc finger nuclease to stimulate a high efficiency of homologous recombination, and chimeric oligonucleotides to promote single base replacement in functional genomic research and plant genetic breeding, are systematically reviewed. Approaches to improve gene targeting efficiency through molecular modification of key pathways in plant homologous recombination are also discussed.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123494416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002271
L. Bo, Zhang He, Zhang Jing, Sun Bo-xing, Chen Lu, Sun Yan-ling, Zhou Xu
Nine prepubertal gilts (JunMu No.1) were randomly allocated to three groups (n =3) and fed with a high level digestive energy (Group H), a low level digestive energy (Group L) and a control group digestive energy (Group M) diet for 14 d, respectively. Free access of water was provided throughout the whole research period. Ovaries and uteruses were collected after 2 weeks energy treatment, and processed for determination of the absolute quantities of insulin-like growth factor receptor (IGF-1R) and epidermal growth factor receptor(EGFR) mRNA by using real-time PCR. The ovaries and uteruses expressed significantly amount of IGF-IR and EGFR mRNA in the Group H than in the Group M and Group L(P0.05). And the ovaries in the Group M expressed significantly larger amount of IGF-1R and EGFR mRNA(P0.05) than that in the Group L. The present study demonstrated that high energy intake markedly enhanced the ovarian and uterine expression of IGF-1R and EGFR in prepubertal gilts, whereas, insufficient energy intake markedly inhibited such expressions. The result suggests that IGF-1R and EGFR may be involved in mediating the effects of energy intake on the development of reproductive system in prepubertal gilts.
{"title":"Effects of dietary energy levels on ovarian and uterine expression of IGF-1R and EGFR mRNA in prepubertal gilts","authors":"L. Bo, Zhang He, Zhang Jing, Sun Bo-xing, Chen Lu, Sun Yan-ling, Zhou Xu","doi":"10.1017/S1479236208002271","DOIUrl":"https://doi.org/10.1017/S1479236208002271","url":null,"abstract":"Nine prepubertal gilts (JunMu No.1) were randomly allocated to three groups (n =3) and fed with a high level digestive energy (Group H), a low level digestive energy (Group L) and a control group digestive energy (Group M) diet for 14 d, respectively. Free access of water was provided throughout the whole research period. Ovaries and uteruses were collected after 2 weeks energy treatment, and processed for determination of the absolute quantities of insulin-like growth factor receptor (IGF-1R) and epidermal growth factor receptor(EGFR) mRNA by using real-time PCR. The ovaries and uteruses expressed significantly amount of IGF-IR and EGFR mRNA in the Group H than in the Group M and Group L(P0.05). And the ovaries in the Group M expressed significantly larger amount of IGF-1R and EGFR mRNA(P0.05) than that in the Group L. The present study demonstrated that high energy intake markedly enhanced the ovarian and uterine expression of IGF-1R and EGFR in prepubertal gilts, whereas, insufficient energy intake markedly inhibited such expressions. The result suggests that IGF-1R and EGFR may be involved in mediating the effects of energy intake on the development of reproductive system in prepubertal gilts.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115957232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002222
Lin Qiu-hua, Hong Bo, T. Zheng, Madlen Chao, Guan Ainong, Yu Jing-juan, Gao Junping
In vitro bulb scales of Lilium longiflorum × L. formosanum were used as the explants to develop a high efficient regeneration system. One hundred percent of regeneration rate was reached through organogenesis on the basal MS media supplement with 1.0 mg/L 6-BA and 1.0 mg/L NAA. And, a genetic transformation system for the lily was also developed using Agrobacterium tumefaciens-mediated method. 12‰ of genetic transformation rate was obtained according to the procedures: the explants were pre-cultured for 3 d, immersed into bacterial suspension (OD600≈0.8) for 5 min, and then co-cultivated for 5 d. The binary vector pBI121 containing Zm401, a maize pollen specific gene, was introduced into the Agrobacterium strain LBA4404 and transformed into the explants using the genetic transformation system, and the integration of the gene into lily genome was confirmed by PCR and PCR-Southern analysis. The results above can be as an important step to get new pollenless materials of lilies.
{"title":"Establishment of regeneration system and transformation of Zm401 gene in Lilium longiflorum × L. formosanum","authors":"Lin Qiu-hua, Hong Bo, T. Zheng, Madlen Chao, Guan Ainong, Yu Jing-juan, Gao Junping","doi":"10.1017/S1479236208002222","DOIUrl":"https://doi.org/10.1017/S1479236208002222","url":null,"abstract":"In vitro bulb scales of Lilium longiflorum × L. formosanum were used as the explants to develop a high efficient regeneration system. One hundred percent of regeneration rate was reached through organogenesis on the basal MS media supplement with 1.0 mg/L 6-BA and 1.0 mg/L NAA. And, a genetic transformation system for the lily was also developed using Agrobacterium tumefaciens-mediated method. 12‰ of genetic transformation rate was obtained according to the procedures: the explants were pre-cultured for 3 d, immersed into bacterial suspension (OD600≈0.8) for 5 min, and then co-cultivated for 5 d. The binary vector pBI121 containing Zm401, a maize pollen specific gene, was introduced into the Agrobacterium strain LBA4404 and transformed into the explants using the genetic transformation system, and the integration of the gene into lily genome was confirmed by PCR and PCR-Southern analysis. The results above can be as an important step to get new pollenless materials of lilies.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129735912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002179
Shi Zhen-dan, Liu Wanli, Zhang Yong-liang, Chen Xue-jin
{"title":"Advances in the development of animal gene transfer","authors":"Shi Zhen-dan, Liu Wanli, Zhang Yong-liang, Chen Xue-jin","doi":"10.1017/S1479236208002179","DOIUrl":"https://doi.org/10.1017/S1479236208002179","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125118209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-08-01DOI: 10.1017/S1479236208002027
Liang Zhong, Chen Hong-quan, Huang Hua-yun, Zha Li-Sha, C. Hua
The intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its SNP was tested by PCR-SSCP in Wanxi White goose(WW)(Anser anser). The carcass trait, meat quality trait and feather-down trait of 116 geese from WW male × Sichuan White goose(A. anser) female were mensurated and analyzed by association with SNP. The results showed that the sequence length of intron 5 of HMGR gene in goose was 704 bp, and the homology between goose and chicken was 44.0%. A SNP site was tested in the intron 5 by PCR-SSCP, and frequencies of allele A and B at the SNP site were 0.7716 and 0.2284, respectively. The effects of genotype AA on increasing chest muscle weight, gland stomach weight (P 0.05), fiber diameter of chest muscle and 1000-downs weight (P 0.01) were significant, and also significant on decreasing down hold-water percentage and chest muscle 45pH (P 0.05). The effect of genotype AB was contrary comparing with genotype AA.
{"title":"SNP in intron 5 of 3-hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR ) gene and its genetic effects on important economic traits in geese","authors":"Liang Zhong, Chen Hong-quan, Huang Hua-yun, Zha Li-Sha, C. Hua","doi":"10.1017/S1479236208002027","DOIUrl":"https://doi.org/10.1017/S1479236208002027","url":null,"abstract":"The intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its SNP was tested by PCR-SSCP in Wanxi White goose(WW)(Anser anser). The carcass trait, meat quality trait and feather-down trait of 116 geese from WW male × Sichuan White goose(A. anser) female were mensurated and analyzed by association with SNP. The results showed that the sequence length of intron 5 of HMGR gene in goose was 704 bp, and the homology between goose and chicken was 44.0%. A SNP site was tested in the intron 5 by PCR-SSCP, and frequencies of allele A and B at the SNP site were 0.7716 and 0.2284, respectively. The effects of genotype AA on increasing chest muscle weight, gland stomach weight (P 0.05), fiber diameter of chest muscle and 1000-downs weight (P 0.01) were significant, and also significant on decreasing down hold-water percentage and chest muscle 45pH (P 0.05). The effect of genotype AB was contrary comparing with genotype AA.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"602 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116333589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002209
Lu Hui-jun, He Wen-qi, Song De-guang, Liu Li-guo, Chang Ling-Zhu, Li Zhi-Ping, Chen Keyan, G. Feng
To identify Porcine haemagglutinating encephalomyelitis virus (HEV) 67N receptor in porcine kidney (PK) cell membranes, the S1 protein of HEV was expressed in Pichia pastoris and purified by Ni 2+ affinity chromatograph. Polyclonal antibodies to HEV were prepared by immunizing rabbits by injecting the purified S1 protein four times. After SDS–polyacrylamide gel electrophoresis (SDS–PAGE), the PK cell membrane proteins were transferred on to nitrocellulose membrane. A virus overlay protein binding assay (VOPBA) was performed using the recombinant S1 protein to identify the protein binding receptor, HEV-S1. The result showed that HEV-S1 protein bound to one band (about 90 kDa) in PK cell membranes. This result is very important for the study of the pathogenic mechanism of HEV.
{"title":"Identification of Porcine haemagglutinating encephalomyelitis virus receptor in PK cell membranes","authors":"Lu Hui-jun, He Wen-qi, Song De-guang, Liu Li-guo, Chang Ling-Zhu, Li Zhi-Ping, Chen Keyan, G. Feng","doi":"10.1017/S1479236208002209","DOIUrl":"https://doi.org/10.1017/S1479236208002209","url":null,"abstract":"To identify Porcine haemagglutinating encephalomyelitis virus (HEV) 67N receptor in porcine kidney (PK) cell membranes, the S1 protein of HEV was expressed in Pichia pastoris and purified by Ni 2+ affinity chromatograph. Polyclonal antibodies to HEV were prepared by immunizing rabbits by injecting the purified S1 protein four times. After SDS–polyacrylamide gel electrophoresis (SDS–PAGE), the PK cell membrane proteins were transferred on to nitrocellulose membrane. A virus overlay protein binding assay (VOPBA) was performed using the recombinant S1 protein to identify the protein binding receptor, HEV-S1. The result showed that HEV-S1 protein bound to one band (about 90 kDa) in PK cell membranes. This result is very important for the study of the pathogenic mechanism of HEV.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131053196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208001915
Gong Wei-hua, Tang Zhonglin, Cui Wentao, Yang Shulin, L. Kui
{"title":"Mapping, tissue distribution and polymorphism of porcine genes encoding retinol binding proteins (RBP1 and RBP4)","authors":"Gong Wei-hua, Tang Zhonglin, Cui Wentao, Yang Shulin, L. Kui","doi":"10.1017/S1479236208001915","DOIUrl":"https://doi.org/10.1017/S1479236208001915","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122415460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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