Pub Date : 2008-04-01DOI: 10.1017/S1479236208002076
Huang Wen-Kun, Guo Jianying, Wan Fanghao, Gao Bi-da, Xie Bingyan
Eupatorium adenophorum (crofton weed) is one of the most widespread invasive species in China. Its genetic diversity and population structure in China were analysed by amplified fragment length polymorphism (AFLP). Three primer pairs were selected for the analysis and 490 bands were produced from 62 E. adenophorum populations selected from five major geographic areas. A total of 328 of the bands showed polymorphism [percentage of polymorphic bands (PPB)=59.4%]. Diversity levels of populations were relatively high (mean expected heterozygosity=0.154, mean Shannon index=0.241). At the regional level, the AMOVA indicated that about 70.25% of variation in the data set was from genotypic variations within populations, whereas 8.04% of the variation was due to regional differences, and the remaining 21.71% to differences among populations within the provincial regions. Cluster analysis based on the unweighted pair-group method using the method of arithmetic averages (UPGMA) grouped the majority of E. adenophorum populations into four main clusters, which correspond to their geographic regions. It is concluded that E. adenophorum spread mainly by wind or water and its genetic diversity level in newly invaded areas was lower than that in formerly colonized areas.
{"title":"AFLP analyses on genetic diversity and structure of Eupatorium adenophorum populations in China","authors":"Huang Wen-Kun, Guo Jianying, Wan Fanghao, Gao Bi-da, Xie Bingyan","doi":"10.1017/S1479236208002076","DOIUrl":"https://doi.org/10.1017/S1479236208002076","url":null,"abstract":"Eupatorium adenophorum (crofton weed) is one of the most widespread invasive species in China. Its genetic diversity and population structure in China were analysed by amplified fragment length polymorphism (AFLP). Three primer pairs were selected for the analysis and 490 bands were produced from 62 E. adenophorum populations selected from five major geographic areas. A total of 328 of the bands showed polymorphism [percentage of polymorphic bands (PPB)=59.4%]. Diversity levels of populations were relatively high (mean expected heterozygosity=0.154, mean Shannon index=0.241). At the regional level, the AMOVA indicated that about 70.25% of variation in the data set was from genotypic variations within populations, whereas 8.04% of the variation was due to regional differences, and the remaining 21.71% to differences among populations within the provincial regions. Cluster analysis based on the unweighted pair-group method using the method of arithmetic averages (UPGMA) grouped the majority of E. adenophorum populations into four main clusters, which correspond to their geographic regions. It is concluded that E. adenophorum spread mainly by wind or water and its genetic diversity level in newly invaded areas was lower than that in formerly colonized areas.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128121811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002040
Wang Xiaobo, Ma Chuanxi, Si Hongqi, He Xian-fang
{"title":"Classification of wheat PPO genes and effect of non-synonymous cSNP on kernel PPO activity","authors":"Wang Xiaobo, Ma Chuanxi, Si Hongqi, He Xian-fang","doi":"10.1017/S1479236208002040","DOIUrl":"https://doi.org/10.1017/S1479236208002040","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125949127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236207001854
Ding Hong-mei, Xu Shi-yong, Shao Gen-bao, Sun Yan, Wang Meng, Liu Honglin
Lentiviruses as gene transfer vectors have been used successfully to transfect mammal embryonic stem cells and germline stem cells, but this has not been attempted in avian primordial germ cells (PGCs). PGCs were isolated from the gonads of Isa-brown chicken embryos at stage 28 and co-cultured with gonadal stroma cells. A lentiviral vector pLenti-CMV-EGFP was constructed and the virus harvested by cotransfecting 293FT cells with the vector and packaging plasmids. Concentrated lentiviruses were used to transfect chicken PGCs, the transfection efficiency was up to 24.19%.
{"title":"Lentiviral vector transfection of chicken embryo primordial germ cells in vitro","authors":"Ding Hong-mei, Xu Shi-yong, Shao Gen-bao, Sun Yan, Wang Meng, Liu Honglin","doi":"10.1017/S1479236207001854","DOIUrl":"https://doi.org/10.1017/S1479236207001854","url":null,"abstract":"Lentiviruses as gene transfer vectors have been used successfully to transfect mammal embryonic stem cells and germline stem cells, but this has not been attempted in avian primordial germ cells (PGCs). PGCs were isolated from the gonads of Isa-brown chicken embryos at stage 28 and co-cultured with gonadal stroma cells. A lentiviral vector pLenti-CMV-EGFP was constructed and the virus harvested by cotransfecting 293FT cells with the vector and packaging plasmids. Concentrated lentiviruses were used to transfect chicken PGCs, the transfection efficiency was up to 24.19%.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125046732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002052
Wang You-ru, Cui Hongzhi, Guo San-dui
The highly-efficient plant binary expression vector pGBIF4ABCVHB was constructed by applying the artificially-modified-synthesized vgbM gene and fusedly-insectidal genes(GFMcryIA and CPTI ). Transgenic tobacco(Nicotiana tabacum) plants were obtained by Agrobacterium mediated transformation, 32 of them were identified that the bivalent genes were inserted into the tobacco genome by PCR amplification detection and Southern blotting analysis. Western blotting analysis proved that vgbM gene was expressed in the transgenic plants. Toxicity assay indicated that fused insectidal genes were expressed activity of pesticadal toxin protein.The net weight of transgenic tobacco plants exceeded 8% of that of non- transgenic ones. Compared to non-transgenic tobacco plants, the transgenic ones were high-yield varieties of insect-resistance.
{"title":"Expression of modified VHb gene and fused insectidal gene in tobacco","authors":"Wang You-ru, Cui Hongzhi, Guo San-dui","doi":"10.1017/S1479236208002052","DOIUrl":"https://doi.org/10.1017/S1479236208002052","url":null,"abstract":"The highly-efficient plant binary expression vector pGBIF4ABCVHB was constructed by applying the artificially-modified-synthesized vgbM gene and fusedly-insectidal genes(GFMcryIA and CPTI ). Transgenic tobacco(Nicotiana tabacum) plants were obtained by Agrobacterium mediated transformation, 32 of them were identified that the bivalent genes were inserted into the tobacco genome by PCR amplification detection and Southern blotting analysis. Western blotting analysis proved that vgbM gene was expressed in the transgenic plants. Toxicity assay indicated that fused insectidal genes were expressed activity of pesticadal toxin protein.The net weight of transgenic tobacco plants exceeded 8% of that of non- transgenic ones. Compared to non-transgenic tobacco plants, the transgenic ones were high-yield varieties of insect-resistance.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115020245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002118
Li-Ming Dou, Lanlan Han, Zhang Jie, KangLai He, Kuijun Zhao, Da-fang Huang, F. Song
A Full-length cry1Ia gene fragment, which obtained by PCR amplification with a pair of primers designed according to cryIa-type gene sequences and DNA from Bacillus thuringiensis Btc008 as template, was introduced into expression vector pET-21b and transformed into Escherichia coli BL21(DE3). Molecular weight of the induced express product was 81 kD. The encoded protein was composed of 719 amino acid residues and the predicted MW was 81.2 kD. The amino acid sequence of the Cry1Ia was very different from those of 12 known Cry1Ia-type proteins. This gene with accession number AF373207 was designated as cry1Ia8 by International Bt Insecticidal Gene Nomenclature Committee. The bioassay results indicated that the Cry1Ia toxin protein showed distinctly insecticidal activity against Ostrinia furnacalis and Plutella xylostella with LC50 of 0.268 μg/g and 2.227 μg/mL, respectively, while it also had insecticidal activity against Leguminivora glycinivorella,but no activity against Pyrrhalta aenescens. The novel cry1Ia8 gene will be new resource for the construction of genetically engineered bacterium and transgenic plant for biocontrol of insect pests. It is also available for screening gene stacks to delay the resistance produce of the pests.
{"title":"Cloning, expression and activity of cry1Ia gene from Bacillus thuringiensis isolate","authors":"Li-Ming Dou, Lanlan Han, Zhang Jie, KangLai He, Kuijun Zhao, Da-fang Huang, F. Song","doi":"10.1017/S1479236208002118","DOIUrl":"https://doi.org/10.1017/S1479236208002118","url":null,"abstract":"A Full-length cry1Ia gene fragment, which obtained by PCR amplification with a pair of primers designed according to cryIa-type gene sequences and DNA from Bacillus thuringiensis Btc008 as template, was introduced into expression vector pET-21b and transformed into Escherichia coli BL21(DE3). Molecular weight of the induced express product was 81 kD. The encoded protein was composed of 719 amino acid residues and the predicted MW was 81.2 kD. The amino acid sequence of the Cry1Ia was very different from those of 12 known Cry1Ia-type proteins. This gene with accession number AF373207 was designated as cry1Ia8 by International Bt Insecticidal Gene Nomenclature Committee. The bioassay results indicated that the Cry1Ia toxin protein showed distinctly insecticidal activity against Ostrinia furnacalis and Plutella xylostella with LC50 of 0.268 μg/g and 2.227 μg/mL, respectively, while it also had insecticidal activity against Leguminivora glycinivorella,but no activity against Pyrrhalta aenescens. The novel cry1Ia8 gene will be new resource for the construction of genetically engineered bacterium and transgenic plant for biocontrol of insect pests. It is also available for screening gene stacks to delay the resistance produce of the pests.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133672912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002258
Liu Ying, Zhu Shi-en, L. Rong, W. Lili, Wang Haiping, Ding Fangrong, Li Jing, Li Song, Dai Yun-ping
The purpose of this study was to evaluate the effects of donor sex, cell cycle synchromization treatments and donor nuclei obtained from fresh or frozen-thawed on in vitro developmental competence of yak-bovine interspecies nuclear transfer (NT) embryos. Bovine (Bos taurus) oocytes were used as recipients, and yak (Bos grummiens) ear fibroblast cell as donors. Results indicated that the development rate of male blastocysts was higher than that of female (56.6% vs 39.5%, P﹤0.05), whereas cleavage and total cell number had no difference between the two groups; No significant difference was observed in the blastocysts development and quality of donor treated with serum starvation or confluence inhibition; And there was no significant difference in embryos development of use fresh or frozen-thaw donor cell, whereas cleavage in group of fresh donor cell was significantly higher than that of frozen-thaw group (54.5% vs 78.2%, P﹤0.05). The results demonstrated that donor sex could impact the in vitro developmental competence of yak-bovine interspecies NT embryos, whereas different cell cycle synchromization treatments and freeze had little influence on it.
本研究旨在探讨供体性别、细胞周期同步处理和新鲜或冻融供体细胞核对牦牛种间核移植(NT)胚胎体外发育能力的影响。以牛(Bos taurus)卵母细胞为受体，牦牛(Bos grummiens)耳成纤维细胞为供体。结果表明，雄性囊胚的发育率高于雌性囊胚(56.6% vs 39.5%， P<0.05)，而卵裂率和总细胞数在两组间无显著差异;血清饥饿和合流抑制对供体囊胚发育和质量无显著影响;使用新鲜供体细胞和冻融供体细胞对胚胎发育无显著影响，但新鲜供体细胞组的卵裂率显著高于冻融组(54.5%比78.2%，P<0.05)。结果表明，供体性别对牦牛种间NT胚胎的体外发育能力有影响，而不同的细胞周期同步处理和冷冻处理对其影响不大。
{"title":"Effects of donor sex and different cell treatments on development of yak–bovine interspecies nuclear transfer embryos","authors":"Liu Ying, Zhu Shi-en, L. Rong, W. Lili, Wang Haiping, Ding Fangrong, Li Jing, Li Song, Dai Yun-ping","doi":"10.1017/S1479236208002258","DOIUrl":"https://doi.org/10.1017/S1479236208002258","url":null,"abstract":"The purpose of this study was to evaluate the effects of donor sex, cell cycle synchromization treatments and donor nuclei obtained from fresh or frozen-thawed on in vitro developmental competence of yak-bovine interspecies nuclear transfer (NT) embryos. Bovine (Bos taurus) oocytes were used as recipients, and yak (Bos grummiens) ear fibroblast cell as donors. Results indicated that the development rate of male blastocysts was higher than that of female (56.6% vs 39.5%, P﹤0.05), whereas cleavage and total cell number had no difference between the two groups; No significant difference was observed in the blastocysts development and quality of donor treated with serum starvation or confluence inhibition; And there was no significant difference in embryos development of use fresh or frozen-thaw donor cell, whereas cleavage in group of fresh donor cell was significantly higher than that of frozen-thaw group (54.5% vs 78.2%, P﹤0.05). The results demonstrated that donor sex could impact the in vitro developmental competence of yak-bovine interspecies NT embryos, whereas different cell cycle synchromization treatments and freeze had little influence on it.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129055786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236207001830
Tian Zhao-ju, Zheng Yu-shu, Li Cuiyan, Hu Jingdong, Zhao Hong-kun
The cDNA of bovine interleukin-18(BoIL-18)was subcloned into pGEX-6P-1 vector and transformed into Escherichia coli BL21(DE3).The recombinant protein was expressed successfully in Escherichia coli by induced with 0.3 mmol/L IPTG for 8 h.SDS-PAGE results indicated that engineering bacteria could express a 44 kD product.And densitometric scanning showed the expressed fusion protein was about 31.8% of total bacterial protein of BL21.The fusion protein was purified by affinity chromatography.Then the biological activity of purified product was assayed.The PBMC(peripheral blood mononuclear cells)proliferation indicated that the BoIL-18 fusion protein could enhance proliferation of PBMC when it was more than 0.10 mg/L.And the ELISA method showed that the BoIL-18 fusion protein could induce IFN-γ production in spleen lymphocyte when it was more than 0.20 mg/L,and the amount of BoIL-18 fusion protein and its inducing effect on IFN-γ had a direct proportion.It can be concluded that the purified BoIL-18 fusion protein has functional activity and can be applied for further studies.
{"title":"Expression and biological activity assay of bovine interleukin-18 fusion protein","authors":"Tian Zhao-ju, Zheng Yu-shu, Li Cuiyan, Hu Jingdong, Zhao Hong-kun","doi":"10.1017/S1479236207001830","DOIUrl":"https://doi.org/10.1017/S1479236207001830","url":null,"abstract":"The cDNA of bovine interleukin-18(BoIL-18)was subcloned into pGEX-6P-1 vector and transformed into Escherichia coli BL21(DE3).The recombinant protein was expressed successfully in Escherichia coli by induced with 0.3 mmol/L IPTG for 8 h.SDS-PAGE results indicated that engineering bacteria could express a 44 kD product.And densitometric scanning showed the expressed fusion protein was about 31.8% of total bacterial protein of BL21.The fusion protein was purified by affinity chromatography.Then the biological activity of purified product was assayed.The PBMC(peripheral blood mononuclear cells)proliferation indicated that the BoIL-18 fusion protein could enhance proliferation of PBMC when it was more than 0.10 mg/L.And the ELISA method showed that the BoIL-18 fusion protein could induce IFN-γ production in spleen lymphocyte when it was more than 0.20 mg/L,and the amount of BoIL-18 fusion protein and its inducing effect on IFN-γ had a direct proportion.It can be concluded that the purified BoIL-18 fusion protein has functional activity and can be applied for further studies.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114416130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236208002003
Yu Zi-Quan, Wang Qian-lan, L. Bin, Zou Xue, Y. Ziniu, S. Ming
{"title":"Bacillus thuringiensis crystal protein toxicity against plant-parasitic nematodes","authors":"Yu Zi-Quan, Wang Qian-lan, L. Bin, Zou Xue, Y. Ziniu, S. Ming","doi":"10.1017/S1479236208002003","DOIUrl":"https://doi.org/10.1017/S1479236208002003","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"316 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116532082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S147923620800199X
Shen Cheng-wen, Huang Yi-huan, Huang Jian-an, Lu Junwu, Liu Chun-lin, Liu De-hua
Genetic diversity and genetic variation of 240 adult plants of four tea populations,Camellia sinensis var.sinensis,C.sinensis var.assamica cv.Duntsa,C.ptilophylla and C.sinensis var.assamica cv.Jianghua in Hunan,were studied by using RAPD markers.The results showed that 226 loci were found by using 21 random primers(10 bp),of which 201 loci were polymorphic(88.9%).The analyzing result of genetic diversity indicated that Shannon's index was 0.43,74.7% of which was within population genetic diversity while 25.3% was among population variation;the gene diversity of total populations(HT)of Nei's index was 0.37,gene diversity within populations(HS)was 0.28,gene diversity among populations(HST)was 0.09 and the coefficient of gene differentiation(GST)among the populations was 0.23,indicating that 76.7% of variation was within populations while 23.3% was among populations,which showed that there was rich genetic variation within population variation,just the same as Shannon's diversity index.Gene flow(Nm)from inter-population was 0.74,which indicated the limited genetic exchange between populations.
{"title":"RAPD analysis on genetic diversity of typical tea populations in Hunan Province","authors":"Shen Cheng-wen, Huang Yi-huan, Huang Jian-an, Lu Junwu, Liu Chun-lin, Liu De-hua","doi":"10.1017/S147923620800199X","DOIUrl":"https://doi.org/10.1017/S147923620800199X","url":null,"abstract":"Genetic diversity and genetic variation of 240 adult plants of four tea populations,Camellia sinensis var.sinensis,C.sinensis var.assamica cv.Duntsa,C.ptilophylla and C.sinensis var.assamica cv.Jianghua in Hunan,were studied by using RAPD markers.The results showed that 226 loci were found by using 21 random primers(10 bp),of which 201 loci were polymorphic(88.9%).The analyzing result of genetic diversity indicated that Shannon's index was 0.43,74.7% of which was within population genetic diversity while 25.3% was among population variation;the gene diversity of total populations(HT)of Nei's index was 0.37,gene diversity within populations(HS)was 0.28,gene diversity among populations(HST)was 0.09 and the coefficient of gene differentiation(GST)among the populations was 0.23,indicating that 76.7% of variation was within populations while 23.3% was among populations,which showed that there was rich genetic variation within population variation,just the same as Shannon's diversity index.Gene flow(Nm)from inter-population was 0.74,which indicated the limited genetic exchange between populations.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114620747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-04-01DOI: 10.1017/S1479236207001969
Xu Zhou-Da, Jin Rui-lian, Gan Qiang, Zeng Hai-Pan, Sun Xuehui, L. Hei, Lu Tie-gang, Liu Guo-zhen
To investigate the wheat transcriptional profile under drought stress, a drought-tolerant variety of wheat ( Triticum aestivum ), Hanxuan 10, was treated with polyethylene glycol (PEG 6000 ) and samples were collected at 0, 1, 6 and 24 h. Complementary DNA was labelled with fluorescent dye and hybridized with the BGI-RiceChip, a whole genome rice gene chip platform, which contains over 60 000 oligos based on the rice genome sequence. Data analysis detected 166, 207 and 328 differentially expressed genes (DGs), respectively, at 1, 6 and 24 h, indicating that the number of DGs increased with the length of the PEG treatment. Functional category analysis showed that the number of DGs related to energy metabolism pathways increased – 4.2%, 8.2% and 16.8%, respectively, as a proportion of the total number of DGs. Most of the photosynthesis-related genes were up-regulated. It is interesting to note that Psbr and ribulose-bisphosphate carboxylase (Rubisco)-coding genes were down-regulated, suggesting their potential role in the response to drought tolerance.
{"title":"Drought-tolerant gene screening in wheat using rice microarray","authors":"Xu Zhou-Da, Jin Rui-lian, Gan Qiang, Zeng Hai-Pan, Sun Xuehui, L. Hei, Lu Tie-gang, Liu Guo-zhen","doi":"10.1017/S1479236207001969","DOIUrl":"https://doi.org/10.1017/S1479236207001969","url":null,"abstract":"To investigate the wheat transcriptional profile under drought stress, a drought-tolerant variety of wheat ( Triticum aestivum ), Hanxuan 10, was treated with polyethylene glycol (PEG 6000 ) and samples were collected at 0, 1, 6 and 24 h. Complementary DNA was labelled with fluorescent dye and hybridized with the BGI-RiceChip, a whole genome rice gene chip platform, which contains over 60 000 oligos based on the rice genome sequence. Data analysis detected 166, 207 and 328 differentially expressed genes (DGs), respectively, at 1, 6 and 24 h, indicating that the number of DGs increased with the length of the PEG treatment. Functional category analysis showed that the number of DGs related to energy metabolism pathways increased – 4.2%, 8.2% and 16.8%, respectively, as a proportion of the total number of DGs. Most of the photosynthesis-related genes were up-regulated. It is interesting to note that Psbr and ribulose-bisphosphate carboxylase (Rubisco)-coding genes were down-regulated, suggesting their potential role in the response to drought tolerance.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121645240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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