Pub Date : 2007-12-01DOI: 10.1017/S1479236207001726
Sun Pei-ming, Liu Yu-tian, W. Qinghua, Wang Zhiliang, Bao En-dong
The localization of heat shock protein 70 (HSP70) and HSP70 mRNA in the heart, liver, lung, kidney, spleen, thymus and cloacal bursa in broilers that were heat stressed for 6 h was conducted using immunohistochemistry and in situ hybridization techniques. Positive HSP70 mRNA signals were detected in the liver and lung, especially in the vessel walls. A weak presence was found in the myocardial cells. No significant signals were observed in spleen, thymus and cloacal bursa. HSP70 was observed in the vessel walls of all investigated broiler tissues. Localizations of HSP70 and HSP70 mRNA suggest that HSP70 could be correlated with cardiovascular function.
{"title":"HSP70 and HSP70 mRNA localization in heat-stressed tissues of broilers","authors":"Sun Pei-ming, Liu Yu-tian, W. Qinghua, Wang Zhiliang, Bao En-dong","doi":"10.1017/S1479236207001726","DOIUrl":"https://doi.org/10.1017/S1479236207001726","url":null,"abstract":"The localization of heat shock protein 70 (HSP70) and HSP70 mRNA in the heart, liver, lung, kidney, spleen, thymus and cloacal bursa in broilers that were heat stressed for 6 h was conducted using immunohistochemistry and in situ hybridization techniques. Positive HSP70 mRNA signals were detected in the liver and lung, especially in the vessel walls. A weak presence was found in the myocardial cells. No significant signals were observed in spleen, thymus and cloacal bursa. HSP70 was observed in the vessel walls of all investigated broiler tissues. Localizations of HSP70 and HSP70 mRNA suggest that HSP70 could be correlated with cardiovascular function.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124487102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-12-01DOI: 10.1017/S1479236207001738
W. Rong, D. Jian, Zhou Zhenming, Ren Li-ping, Meng Qing-xiang
Three Luxi adult yellow steers were used to isolate and culture intramuscular preadipocyte in vitro and examine factors influencing their proliferation and differentiation. The intramuscular preadipocyte cells were taken from adipose tissues within muscles between sixth and seventh rib and cultured after digestion by collagenase Ⅰ. The results showed that the separated cells were highly homogeneous, proliferative and double timed within 62 h. When the confluent preadipocytes were treated with differentiation medium, small lipid droplets began to appear on day 2 and the number of lipid droplets rapidly increased around the nuclei on day 6. Their dynamic morphological changes, growth curve, oil O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the intramuscular preadipocytes replayed their proliferation and differentiation process in vitro, especially, the proportion of cultured diploid preadipocytes reached to more than 90% after 6 run of repeated cultures were performed. In conclusion, the current study confirms that functionally active preadipocytes indeed present within muscles of China adult local breed cattle. The cell strains will be potentially used as a useful model for further understanding of the mechanism by which the intramuscular adipose is deposed in the tissues, and for improving the beef quality based on local breed beef cattle.
{"title":"Isolation and in vitro culture of intramuscular pre-adipocytes from Luxi adult Yellow cattle","authors":"W. Rong, D. Jian, Zhou Zhenming, Ren Li-ping, Meng Qing-xiang","doi":"10.1017/S1479236207001738","DOIUrl":"https://doi.org/10.1017/S1479236207001738","url":null,"abstract":"Three Luxi adult yellow steers were used to isolate and culture intramuscular preadipocyte in vitro and examine factors influencing their proliferation and differentiation. The intramuscular preadipocyte cells were taken from adipose tissues within muscles between sixth and seventh rib and cultured after digestion by collagenase Ⅰ. The results showed that the separated cells were highly homogeneous, proliferative and double timed within 62 h. When the confluent preadipocytes were treated with differentiation medium, small lipid droplets began to appear on day 2 and the number of lipid droplets rapidly increased around the nuclei on day 6. Their dynamic morphological changes, growth curve, oil O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the intramuscular preadipocytes replayed their proliferation and differentiation process in vitro, especially, the proportion of cultured diploid preadipocytes reached to more than 90% after 6 run of repeated cultures were performed. In conclusion, the current study confirms that functionally active preadipocytes indeed present within muscles of China adult local breed cattle. The cell strains will be potentially used as a useful model for further understanding of the mechanism by which the intramuscular adipose is deposed in the tissues, and for improving the beef quality based on local breed beef cattle.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131078532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-12-01DOI: 10.1017/S1479236207001957
Wang Jin-chan, Qian Wei-wei, Zhang Long-xian, Ning Chang-shen, Jian Fuchun, Zhao Jinfeng, W. Ming
The mitochondrial functional protein alternative oxidase(AOX)gene was used as the marker for analysing phylogentic relationship of the Cryptosporidium isolates.The AOX genes were characterized,and the phylogentic tree was established from the Cryptosporidium isolates,and compared with that of 18S rRNA and HSP70 gene sequences,then determined whether the AOX gene was suitable for phylogentic analysis of the genus of Cryptosporidium or not.The results revealed that the genus Cryptosporidium contained the phylogenetical distinct species C.parvum,C.hominis,C.suis and C.baileyi,which were consistent with the biological characteristics and host specificity up to date.The Cryptosporidium species formed two clades,one included C.hominis,C.suis,C.parvum cattle genotype and C.parvum mouse genotype and the other included C.meleagridis and C.baileyi isolates.Within C.parvum,both the mouse genotype isolate and the pig genotypeⅠ(already known as C.suis)isolate differed from cattle genotype and human genotype(already known as C.hominis)based on the nucleotide sequences alignment.The identity of the AOX gene sequences between C.meleagridis and C.baileyi was more distinguishedly than that of between C.meleagridis and C.parvum,and the phylogentic trees showed that C.meleagridis had a closer phylogenetic affinity with the C.baileyi than that with C.parvum,this result was inconsistent with the phylogentic analysis results from 18S rRNA and HSP70 gene sequences,respectively.Present result suggested that the AOX gene is not only equally suitable for the phylogentic analysis of Cryptosporidium,but also provides an approach to identify new genes heredity of Cryptosporidium.
{"title":"Phylogenetic analysis of Cryptosporidium isolates in Henan","authors":"Wang Jin-chan, Qian Wei-wei, Zhang Long-xian, Ning Chang-shen, Jian Fuchun, Zhao Jinfeng, W. Ming","doi":"10.1017/S1479236207001957","DOIUrl":"https://doi.org/10.1017/S1479236207001957","url":null,"abstract":"The mitochondrial functional protein alternative oxidase(AOX)gene was used as the marker for analysing phylogentic relationship of the Cryptosporidium isolates.The AOX genes were characterized,and the phylogentic tree was established from the Cryptosporidium isolates,and compared with that of 18S rRNA and HSP70 gene sequences,then determined whether the AOX gene was suitable for phylogentic analysis of the genus of Cryptosporidium or not.The results revealed that the genus Cryptosporidium contained the phylogenetical distinct species C.parvum,C.hominis,C.suis and C.baileyi,which were consistent with the biological characteristics and host specificity up to date.The Cryptosporidium species formed two clades,one included C.hominis,C.suis,C.parvum cattle genotype and C.parvum mouse genotype and the other included C.meleagridis and C.baileyi isolates.Within C.parvum,both the mouse genotype isolate and the pig genotypeⅠ(already known as C.suis)isolate differed from cattle genotype and human genotype(already known as C.hominis)based on the nucleotide sequences alignment.The identity of the AOX gene sequences between C.meleagridis and C.baileyi was more distinguishedly than that of between C.meleagridis and C.parvum,and the phylogentic trees showed that C.meleagridis had a closer phylogenetic affinity with the C.baileyi than that with C.parvum,this result was inconsistent with the phylogentic analysis results from 18S rRNA and HSP70 gene sequences,respectively.Present result suggested that the AOX gene is not only equally suitable for the phylogentic analysis of Cryptosporidium,but also provides an approach to identify new genes heredity of Cryptosporidium.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134159935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-12-01DOI: 10.1017/S1479236207001672
Ye Hong-xia, Guo Ze-jian, L. Mei, Xu Xiao-hui, B. Jinsong, Shen Sheng-quan
{"title":"Variation in biological characteristics of purified pea ferritin (Fer) transgenic rice","authors":"Ye Hong-xia, Guo Ze-jian, L. Mei, Xu Xiao-hui, B. Jinsong, Shen Sheng-quan","doi":"10.1017/S1479236207001672","DOIUrl":"https://doi.org/10.1017/S1479236207001672","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123788123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S1479236207001507
Yang Hong-wen, Zheng Rong, Li Feng-e, Jiang Siwen
cDNA of the porcine small adipocyte factor 1 ( SMAF1 ) gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed according to the conserved sequences between human and mouse genes. The cDNA (GenBank accession No. DQ191892) containing a complete encoding region was 256 bp in length, sharing 86 and 78% identity with that of human and mouse, respectively. Comparison of the deduced amino acid sequence between porcine SMAF1 and the human, mouse, cattle and rat protein showed that the amino acid similarity was 81, 67, 84 and 70%, respectively. The results of semi-quantitative RT-PCR showed that the porcine gene was expressed abundantly in adipose tissue, and at a significantly lower level in lean-type pigs (Large White pigs) than in lard-type pigs (Meishan pigs) at the age of 4 months ( P SMAF1 gene may have similar functions as in other species, that is, it may regulate adipogenesis and/or adipocyte function.
{"title":"Cloning and expression analysis of porcine small adipocyte factor 1 ( SMAF1 ) gene","authors":"Yang Hong-wen, Zheng Rong, Li Feng-e, Jiang Siwen","doi":"10.1017/S1479236207001507","DOIUrl":"https://doi.org/10.1017/S1479236207001507","url":null,"abstract":"cDNA of the porcine small adipocyte factor 1 ( SMAF1 ) gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed according to the conserved sequences between human and mouse genes. The cDNA (GenBank accession No. DQ191892) containing a complete encoding region was 256 bp in length, sharing 86 and 78% identity with that of human and mouse, respectively. Comparison of the deduced amino acid sequence between porcine SMAF1 and the human, mouse, cattle and rat protein showed that the amino acid similarity was 81, 67, 84 and 70%, respectively. The results of semi-quantitative RT-PCR showed that the porcine gene was expressed abundantly in adipose tissue, and at a significantly lower level in lean-type pigs (Large White pigs) than in lard-type pigs (Meishan pigs) at the age of 4 months ( P SMAF1 gene may have similar functions as in other species, that is, it may regulate adipogenesis and/or adipocyte function.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"38 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116657094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S1479236207001611
He Zi-fu, Y. Hao, Mao Ming-Jie, Luo Fangfang, Lin Yi-han, Wang Sui-Tao
A yellow leaf curl disease with chlorotic and yellowish leaves, upward leaf curling and stunting symptoms was observed on tomato in Shantou city of Guangdong province. A virus isolate BS was obtained from a diseased tomato plant. The complete DNA-A sequence of the virus isolate BS was determined to be 2740 nucleotides long, with all the characteristic features of begomovirus genome organization. BS DNA-A encoded six potential open reading frames (ORFs), with two ( AV1 and AV2 ) in virus sense and four ( AC1 , AC2 , AC3 and AC4 ) in complementary sense, and contained an intergenic region of 269 nucleotides. The results of BLAST searches showed that BS DNA-A had higher sequence identity with reported begomoviruses in Asia than with those in America and Africa. Further sequence comparisons indicated that BS was most closely related to the isolate of Tomato leaf curl Taiwan virus (ToLCTWV-[Taiwan]) with a sequence identity of 97.7%. Nucleotide sequence identities of AV1 , AV2 , AC1 , AC2 , AC3 , AC4 and intergenic region (IR) between BS and ToLCTWV-[Taiwan] were 98.6, 98.0, 98.0, 97.5, 96.3, 98.6 and 96.6%, respectively, while that of the six ORF-encoded proteins between BS and ToLCTWV-[Taiwan] were 97.7, 99.1, 97.5, 95.6, 91.8 and 99.0%, respectively. Phylogenetic analysis based on the DNA-A sequences has also indicated that BS is most closely related to ToLCTWV-[Taiwan], forming a branch with ToLCTWV-[Taiwan], Tomato leaf curl Guangdong virus and Tomato yellow leaf curl Guangdong virus . The above results demonstrate that BS is an isolate of ToLCTWV.
{"title":"Tomato yellow leaf curl disease in Guangdong is caused by Tomato leaf curl Taiwan virus","authors":"He Zi-fu, Y. Hao, Mao Ming-Jie, Luo Fangfang, Lin Yi-han, Wang Sui-Tao","doi":"10.1017/S1479236207001611","DOIUrl":"https://doi.org/10.1017/S1479236207001611","url":null,"abstract":"A yellow leaf curl disease with chlorotic and yellowish leaves, upward leaf curling and stunting symptoms was observed on tomato in Shantou city of Guangdong province. A virus isolate BS was obtained from a diseased tomato plant. The complete DNA-A sequence of the virus isolate BS was determined to be 2740 nucleotides long, with all the characteristic features of begomovirus genome organization. BS DNA-A encoded six potential open reading frames (ORFs), with two ( AV1 and AV2 ) in virus sense and four ( AC1 , AC2 , AC3 and AC4 ) in complementary sense, and contained an intergenic region of 269 nucleotides. The results of BLAST searches showed that BS DNA-A had higher sequence identity with reported begomoviruses in Asia than with those in America and Africa. Further sequence comparisons indicated that BS was most closely related to the isolate of Tomato leaf curl Taiwan virus (ToLCTWV-[Taiwan]) with a sequence identity of 97.7%. Nucleotide sequence identities of AV1 , AV2 , AC1 , AC2 , AC3 , AC4 and intergenic region (IR) between BS and ToLCTWV-[Taiwan] were 98.6, 98.0, 98.0, 97.5, 96.3, 98.6 and 96.6%, respectively, while that of the six ORF-encoded proteins between BS and ToLCTWV-[Taiwan] were 97.7, 99.1, 97.5, 95.6, 91.8 and 99.0%, respectively. Phylogenetic analysis based on the DNA-A sequences has also indicated that BS is most closely related to ToLCTWV-[Taiwan], forming a branch with ToLCTWV-[Taiwan], Tomato leaf curl Guangdong virus and Tomato yellow leaf curl Guangdong virus . The above results demonstrate that BS is an isolate of ToLCTWV.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"88 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128001998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S1479236207001428
Chen Yong-hua, Zhao Sen, Yan Qin-quan, Li Yang-sheng, Wu Xing-rong, Xiao Guo-ying
Gene expression profiles between submergence rice ( Oryza sativa ssp. indica ) varieties, tolerant FR13A and sensitive IR39595-503-2-1-2, under submergence stress were identified using differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR). A total of 1428 bands were amplified with 40 pairs of primers, of which 102 were significantly different between the two lines. The differential display ratio was 7.1%. Among 42 differential display bands derived from the submergence tolerant variety, the expression of seven fragments was confirmed by Northern blot analysis. The analysis of their sequences indicated that four of them showed high homology with genes related to a water stress response: genes encoding ATP-binding protein, isocitrate dehydrogenase, NADH dehydrogenase and terminal acetyltransferase, respectively. The remaining three fragments were novel cDNA fragments.
{"title":"Tolerance of submergence in rice: gene studies using differential display technique","authors":"Chen Yong-hua, Zhao Sen, Yan Qin-quan, Li Yang-sheng, Wu Xing-rong, Xiao Guo-ying","doi":"10.1017/S1479236207001428","DOIUrl":"https://doi.org/10.1017/S1479236207001428","url":null,"abstract":"Gene expression profiles between submergence rice ( Oryza sativa ssp. indica ) varieties, tolerant FR13A and sensitive IR39595-503-2-1-2, under submergence stress were identified using differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR). A total of 1428 bands were amplified with 40 pairs of primers, of which 102 were significantly different between the two lines. The differential display ratio was 7.1%. Among 42 differential display bands derived from the submergence tolerant variety, the expression of seven fragments was confirmed by Northern blot analysis. The analysis of their sequences indicated that four of them showed high homology with genes related to a water stress response: genes encoding ATP-binding protein, isocitrate dehydrogenase, NADH dehydrogenase and terminal acetyltransferase, respectively. The remaining three fragments were novel cDNA fragments.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127946439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S147923620700157X
Yang Chi-Chun, Yu Jing-juan, Z. Qian, Zhu Dengyun, Ao Guang-ming
XY355 promoter, a petal-specific promoter, was amplified from the genome of rape(Brassica napus ) by PCR. The plant expression vector pXY60,which contained XY355 promoter and the maize Lc regulatory gene, was constructed. The vector was respectively transfered into tobacco(Nicotiana tabacum)and petunia(Petunia hybrida )by Agrobacterium tumefaciens-mediated method. The results of PCR analysis among these regenerated plants indicated that Lc gene was integrated into the genomes of transgenic tobacco and petunia, respectively. The flower color of transgenic plants had been changed, the color of transgenic tobacco was changed from light red to red and that of transgenic petunia was changed from white to light purple.
{"title":"Influence of maize Lc regulatory gene on flower colour of transgenic tobacco and petunia","authors":"Yang Chi-Chun, Yu Jing-juan, Z. Qian, Zhu Dengyun, Ao Guang-ming","doi":"10.1017/S147923620700157X","DOIUrl":"https://doi.org/10.1017/S147923620700157X","url":null,"abstract":"XY355 promoter, a petal-specific promoter, was amplified from the genome of rape(Brassica napus ) by PCR. The plant expression vector pXY60,which contained XY355 promoter and the maize Lc regulatory gene, was constructed. The vector was respectively transfered into tobacco(Nicotiana tabacum)and petunia(Petunia hybrida )by Agrobacterium tumefaciens-mediated method. The results of PCR analysis among these regenerated plants indicated that Lc gene was integrated into the genomes of transgenic tobacco and petunia, respectively. The flower color of transgenic plants had been changed, the color of transgenic tobacco was changed from light red to red and that of transgenic petunia was changed from white to light purple.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"57 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129305852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S1479236207001465
W. Xiao, Z. Le, Xu Fu-shou, Z. LiHan, Xie Guanlin
An immuno-capture polymerase chain reaction (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli (AAC), the causal organism of bacterial fruit blotch (BFB) of watermelon, was developed by combining the immunosorbent enrichment (ISE) method with classical PCR and comparing with the direct PCR and growth check methods. The results showed that all A. avenae subsp. citrulli strains tested have produced 360 bp specific fragments using IC-PCR and direct PCR methods, while other strains from 10 different genera showed negative PCR results. The minimum detection concentration was about 50–100 cfu/ml and 104 cfu/ml, respectively. The IC-PCR sensitivity was 100 times higher than that of direct PCR. The examination of seven batches of different melon seeds from the markets by IC-PCR showed that one cantaloupe, two honeydew melon and two watermelon seed varieties carried the pathogen, indicating that the IC-PCR is an accurate, sensitive, rapid and low-cost technique.
{"title":"Immuno-capture PCR method for detecting Acidovorax avenae subsp. citrulli from watermelon","authors":"W. Xiao, Z. Le, Xu Fu-shou, Z. LiHan, Xie Guanlin","doi":"10.1017/S1479236207001465","DOIUrl":"https://doi.org/10.1017/S1479236207001465","url":null,"abstract":"An immuno-capture polymerase chain reaction (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli (AAC), the causal organism of bacterial fruit blotch (BFB) of watermelon, was developed by combining the immunosorbent enrichment (ISE) method with classical PCR and comparing with the direct PCR and growth check methods. The results showed that all A. avenae subsp. citrulli strains tested have produced 360 bp specific fragments using IC-PCR and direct PCR methods, while other strains from 10 different genera showed negative PCR results. The minimum detection concentration was about 50–100 cfu/ml and 104 cfu/ml, respectively. The IC-PCR sensitivity was 100 times higher than that of direct PCR. The examination of seven batches of different melon seeds from the markets by IC-PCR showed that one cantaloupe, two honeydew melon and two watermelon seed varieties carried the pathogen, indicating that the IC-PCR is an accurate, sensitive, rapid and low-cost technique.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121055722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-08-01DOI: 10.1017/S1479236207001404
Wang Jinlong, Yang Hong, Wu Ting-ting
This research focused on the effect of distant hybridization to improve the flesh quality of offspring from Oreochromis aurea (♀)× Siniperca chuatsi (♂). The proportions of different tissues and percentages of nutrient components were compared between fish of the F 3 generation and O. aurea and S. chuatsi . The results showed that the proportion of flesh in F 3 hybrids was significantly lower than that in O. aurea . Crude lipid content was lower and crude protein content significantly higher in the F 3 generation. Concentrations of four kinds of amino acids important for flavour (DAA) were higher in the F 3 generation than in O. aurea , and the total DAA was significantly higher. Furthermore, the other amino acid contents and essential amino acid index of the F 3 generation were intermediate between O. aurea and S. chuatsi . We conclude that some effects of hybridization have induced flesh quality improvement.
{"title":"Comparison of percentages of tissues and nutrients between parents and F3 offspring of Oreochromis aurea×Siniperca chuatsi","authors":"Wang Jinlong, Yang Hong, Wu Ting-ting","doi":"10.1017/S1479236207001404","DOIUrl":"https://doi.org/10.1017/S1479236207001404","url":null,"abstract":"This research focused on the effect of distant hybridization to improve the flesh quality of offspring from Oreochromis aurea (♀)× Siniperca chuatsi (♂). The proportions of different tissues and percentages of nutrient components were compared between fish of the F 3 generation and O. aurea and S. chuatsi . The results showed that the proportion of flesh in F 3 hybrids was significantly lower than that in O. aurea . Crude lipid content was lower and crude protein content significantly higher in the F 3 generation. Concentrations of four kinds of amino acids important for flavour (DAA) were higher in the F 3 generation than in O. aurea , and the total DAA was significantly higher. Furthermore, the other amino acid contents and essential amino acid index of the F 3 generation were intermediate between O. aurea and S. chuatsi . We conclude that some effects of hybridization have induced flesh quality improvement.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129700641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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