Veterinary parasitology最新文献

{"title":"Assessing the efficacy of three trypanocidal drugs in controlling Trypanosoma evansi","authors":"Somaia Abouakkada ,&nbsp;Nadia Labn ,&nbsp;Safeya Henidy ,&nbsp;Samah S. Oda ,&nbsp;Mohamed E. Abd El-Hack ,&nbsp;Fatima S. Alaryani ,&nbsp;Aishah E. Albalawi ,&nbsp;Kamlah Ali Majrashi ,&nbsp;Amira Dewair","doi":"10.1016/j.vetpar.2025.110658","DOIUrl":"10.1016/j.vetpar.2025.110658","url":null,"abstract":"<div><div><em>Trypanosoma evansi</em> is an emerging zoonotic threat causing significant economic and health issues, especially in tropical and subtropical regions. Due to limited effective trypanocidal agents and increasing drug resistance, this investigation sought to evaluate the therapeutic efficacy of quinapyramine sulfate and chloride, melarsamine hydrochloride, and diminazene aceturate in 72 female Swiss albino mice. Each drug was administered at two dosages: quinapyramine (3 and 6 mg/kg), melarsamine hydrochloride (0.25 and 0.5 mg/kg), and diminazene aceturate (3.5 and 7 mg/kg). The efficacy was evaluated based on parasitaemia, relapse patterns, body weight changes, hematological and biochemical profiles, postmortem and histopathological findings. The results showed quinapyramine was ineffective at both dosages. However, doubling the therapeutic doses of melarsamine hydrochloride and diminazene aceturate enhanced their efficacy, although parasitaemia relapsed in all cases. Blood biochemical analyses revealed infection-induced anemia, leukocytosis, eosinophilia, and significant reductions in total protein, serum albumin, and globulin levels. Histopathological examination revealed evidence of degenerative pathology and tissue injury within the hepatic, splenic, cardiac, and renal parenchyma in all experimental groups with varying degrees. These findings suggest that melarsamine hydrochloride, at higher doses, may offer a more effective treatment for <em>T. evansi</em> infection, although relapse remains a challenge.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110658"},"PeriodicalIF":2.2,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and specific detection of Babesia vogeli using RPA/CRISPR-Cas12a: A feasible field-friendly diagnostic for canine babesiosis","authors":"Suphaporn Paenkaew ,&nbsp;Thippaporn Euppayo ,&nbsp;Rongdej Tungtrakanpoung ,&nbsp;Worapan Teapunvong ,&nbsp;Korakot Nganvongpanit ,&nbsp;Kittisak Buddhachat","doi":"10.1016/j.vetpar.2025.110660","DOIUrl":"10.1016/j.vetpar.2025.110660","url":null,"abstract":"<div><div><em>Babesia vogeli</em> is a protozoan parasite causing canine babesiosis, a tick-borne disease prevalent in tropical and subtropical regions. Its microscopic identification is challenging due to morphological similarity with other <em>Babesia</em> spp., and serological assays often yield inaccurate results. To address this issue, we developed a rapid, equipment-minimal diagnostic method combining recombinase polymerase amplification (RPA) with CRISPR/Cas12a (RPA/CRISPR-cas12a) for <em>B. vogeli</em>-specific detection. The RPA assay enables DNA amplification for both <em>B. vogeli</em> and <em>Hepatozoon canis</em>, while CRISPR/Cas12a using gRNA_Bab ensures specificity for <em>B. vogeli</em>, even in co-infections and other pathogens. This approach detects as few as 10⁵ copies within two hours for both readout platforms such as fluorescence and lateral flow dipstick (LFD). Forty canine blood samples were detected by RPA/CRISPR-cas12a to examine its performance. Results showed high concordance with qPCR-high resolution melting (HRM) (Cohen’s kappa: 0.93 for fluorescence, 0.81 for LFD), outperforming conventional PCR. The clinical sensitivity and specificity of RPA/CRISPR-cas12a were 100 % and 96.8 %, respectively and the concordance with qPCR-HRM was 97.5 %. RPA/CRISPR-cas12a for <em>Babesi</em>a spp. detection provided a simple, rapid, and accurate method, demonstrating promise for point-of-care diagnosis of canine babesiosis in resource-limited settings. This method showed high potential as a practical diagnostic tool in veterinary clinics, with accelerated surveillance to control outbreaks of <em>Babesia</em>-associated canine babesiosis.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110660"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of in vitro insecticide selection pressure to a susceptible strain of the sheep blowfly reveals different rates of resistance development and cross-resistance patterns","authors":"Andrew C. Kotze ,&nbsp;Neil H. Bagnall ,&nbsp;Angela P. Ruffell ,&nbsp;Nicholas M. Rolls","doi":"10.1016/j.vetpar.2025.110661","DOIUrl":"10.1016/j.vetpar.2025.110661","url":null,"abstract":"<div><div>The present study aimed to investigate the development of resistance to the pyrimidine derivative dicyclanil and the neonicotinoid imidacloprid in the sheep blowfly, <em>Lucilia cuprina</em>. We applied laboratory-based insecticide selection pressure to a susceptible strain and measured resistance patterns that emerged to all of the currently-available blowfly control chemicals. The dynamics of resistance emergence was quite different for the two compounds. Resistance to imidacloprid emerged after 4 rounds of selection, increased up to 9 rounds before then remaining relatively constant at almost 10-fold until the termination of the experiment after 15 rounds. Dicyclanil resistance did not emerge until the 13th round of selection, and then increased to approximately 3.5-fold at 15 rounds. This faster development of resistance to imidacloprid is in agreement with observations of resistance emergence in field populations of the blowfly over the last 25 years. The imidacloprid-selected strain also developed resistance towards dicyclanil (3.1-fold). Similarly, the dicyclanil-selected strain also showed resistance to imidacloprid (4.8-fold). This level of resistance to imidacloprid in the dicyclanil-selected strain (4.8-fold) was slightly higher than the level of resistance to dicyclanil itself (3.5-fold). The study provides insights into the dynamics of resistance emergence to different compounds in the sheep blowfly and describes cross-resistance patterns with important implications for chemical rotation strategies. In demonstrating the emergence of resistance to two chemical classes after exposure to single classes, this study illustrates the need to examine the potential impact of resistance mechanisms that can act across multiple insecticide classes when designing insecticide-use strategies for parasite control.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110661"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal parasites in dogs and their association with clinical manifestations of canine visceral leishmaniasis","authors":"Allan de Jesus Mendonça Severino ,&nbsp;Dharliton Soares Gomes ,&nbsp;André Tetzl Costa ,&nbsp;Maykelin Fuentes Zaldívar ,&nbsp;Nathália Moreira Teodoro de Oliveira ,&nbsp;Bianca Leal de Oliveira ,&nbsp;Paulo Ricardo Silva Coelho ,&nbsp;Felipe Bisaggio Pereira ,&nbsp;Paulo Ricardo de Oliveira Paes ,&nbsp;João Carlos França-Silva ,&nbsp;Rodolfo Cordeiro Giunchetti ,&nbsp;Stefan Michael Geiger","doi":"10.1016/j.vetpar.2025.110659","DOIUrl":"10.1016/j.vetpar.2025.110659","url":null,"abstract":"<div><div>Dogs are companion animals commonly present in spaces shared with humans. However, this close contact may facilitate the transmission of zoonoses, such as canine visceral leishmaniasis (CVL). Additionally, dogs are potential hosts of endoparasites that can influence the clinical manifestations of CVL due to immunological interference occurring in parasitic coinfections. This study aimed to investigate the occurrence of endoparasites in domestic dogs seropositive for CVL and associate their clinical manifestations with the presence of intestinal helminths. We analyzed 47 seropositive dogs (51.06 % males; 48.9 % females; 80.8 % of undefined breed) that underwent clinical examination, blood count, euthanasia, necropsy, and coproparasitological diagnosis. Dogs were grouped into three clinical categories: low (40.4 %), medium (34.0 %), and severe (25.5 %) based on a modified version of the LeishVet clinical staging system. Coproparasitological results indicated <em>Ancylostoma</em> sp. and <em>Giardia</em> sp. as the most prevalent. Based on necropsy, <em>Ancylostoma</em> sp. and <em>Dipylidium caninum</em> were the most common helminths. Dogs with CVL and intestinal helminths frequently had macrocytic hypochromic (p = 0.0455) and microcytic hypochromic (p = 0.01963) anemia. We also found that <em>Ancylostoma</em> sp. was associated with more severe CVL clinical staging (odds ratio [OR] = 3.3621; 95 % confidence interval [CI] = 2.6413–4.2796) and negatively associated with red blood cell counts (OR = 0.9278; CI = 0.8702–0.9893). We conclude that intestinal helminth infections in dogs with CVL may represent an important aggravating factor for the disease’s clinical progression.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110659"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Green synthesis and larvicidal activity of ZnO nanoparticles against Lucilia sericata larvae using essential oil bio-reductants” [Vet. Parasitol. 340 (2025) 110633]","authors":"Hatice Tosyagülü Çeli̇k ,&nbsp;Nilüfer Vural ,&nbsp;Sibel Kaymak","doi":"10.1016/j.vetpar.2025.110655","DOIUrl":"10.1016/j.vetpar.2025.110655","url":null,"abstract":"","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110655"},"PeriodicalIF":2.2,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double antigen sandwich ELISA for detection of antibodies against Babesia bovis.","authors":"María Evangelina Primo,&nbsp;Beatriz Susana Valentini,&nbsp;Matilde Mazzucco Panizza,&nbsp;Nicolás Morel,&nbsp;Carolina Soledad Thompson","doi":"10.1016/j.vetpar.2025.110657","DOIUrl":"10.1016/j.vetpar.2025.110657","url":null,"abstract":"<div><div><em>Babesia bovis</em> is a hemoparasite responsible for bovine babesiosis, an economically important tickborne disease widespread in tropical and subtropical regions of the world. Serological tests are used in calves to evaluate the need for vaccination and the immunity achieved after vaccination. We developed, validated, and evaluated under field conditions a double-antigen sandwich ELISA (dasELISA) using a recombinant major surface antigen 2c (MSA2c) from a pathogenic and an attenuated <em>B. bovis</em> strain. The tests were validated using a panel of 1057 serum samples of known status (217 known-positive and 840 known-negative). Samples from 116 herds (n = 2771) were evaluated by indirect ELISA (iELISA) and dasELISA. Samples with disagreement between ELISAs (231 out of 594, 38.9 %) were analyzed by IFAT. The dasELISA cutoff was ≥ 52 %P, with a diagnostic sensitivity of 94.5 % (95 % CI, 90.5–97.1) and a diagnostic specificity of 96.4 % (95 % CI, 94.9–97.6). The area under the curve (AUC) was assessed to be 0.979, which corresponds to an excellent ability of the assay to discriminate truly infected from truly uninfected animals. Concordance among dasELISA and iELISA was 78.6 %, with a κ value of 0.567 (95 % CI = 0.537–0.598); meanwhile, 57 % and 43 % of the IFAT results agreed with dasELISA and iELISA, respectively. The dasELISA could be useful for large-scale detection of anti–<em>B. bovis</em> antibodies in calves pre- and post-vaccination and for seroepidemiologic investigations, given its appropriate sensitivity and specificity and the simplicity of production.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110657"},"PeriodicalIF":2.2,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasciola hepatica vaccine based on Kunitz-type molecule reduces adult worm fecundity in experimentally infected sheep","authors":"María Ahumada ,&nbsp;Lorena Guasconi ,&nbsp;Belkys Angélica Maletto ,&nbsp;Constanza Marín ,&nbsp;Santiago Daniel Palma ,&nbsp;Cesar Iván Pruzzo ,&nbsp;Corvo Ileana ,&nbsp;Gabriel Caffe ,&nbsp;Ana María Martin ,&nbsp;Laura Chiapello ,&nbsp;Laura Cervi","doi":"10.1016/j.vetpar.2025.110654","DOIUrl":"10.1016/j.vetpar.2025.110654","url":null,"abstract":"<div><div>Fasciolosis is a widespread and continuously expanding helminthiasis caused by the trematode <em>Fasciola hepatica</em>. Sheep and cattle are the primary definitive hosts of <em>F. hepatica</em> and are economically significant hosts for this pathogen worldwide. <em>F. hepatica</em> is not only a major threat to livestock but also an important neglected zoonosis. Reports of anthelmintic resistance in <em>F. hepatica</em> emphasize the urgent need for the development of an effective vaccine. Such a vaccine would reduce the impact and spread of the disease by decreasing the number of viable eggs, as well as reducing the adult worm population, ultimately leading to less liver damage. In a previous study, we demonstrated the ability of the <em>F. hepatica</em> Kunitz-type molecule synthetic (sFhKTM), formulated with a liquid crystal nanostructure created through the self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) to provide protection against <em>F. hepatica</em> in infected mice. In this study, we assessed the efficacy of the vaccine sFhKT/CpG-ODN/Coa-ASC16 in sheep. The formulation containing the highest sFhKT dose was the most effective, significantly reducing fecal egg counts by 81.6 % (<em>p</em> &lt; 0.0001). It also reduced worm burden by 55.7 % (<em>p</em> = 0.179), although this difference was not statistically significant. The addition of Cathepsin L3 (FhCL3) further reduced fecal egg counts (89.1 %, <em>p</em> &lt; 0.0001) but resulted in a lower reduction in worm burden (24.06 %). Sheep vaccinated with sFhKT/CpG-ODN/Coa-ASC16 exhibited slightly less hepatic damage than non-vaccinated animals, with histological lesions characterized by increased inflammatory infiltrates. The experimental vaccine FhKT/CpG-ODN/Coa-ASC16 induced non-significantly greater IgG titers in immunized sheep compared to non-vaccinated controls. The <em>variation</em> in efficacy observed between the sFhKT doses highlights the need for additional trials using higher protein concentrations.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110654"},"PeriodicalIF":2.2,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145584586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metagenomic profiling of microbial pathogen diversity in Haemaphysalis longicornis and Hyalomma dromedarii ticks","authors":"Luo Jin ,&nbsp;Lan Xinting ,&nbsp;Zhao Shuaiyang ,&nbsp;Ren Qiaoyun ,&nbsp;Muhammad Kashif Obaid ,&nbsp;Diao Peiwen ,&nbsp;Guan Guiquan ,&nbsp;Liu Guangyuan ,&nbsp;Yin Hong","doi":"10.1016/j.vetpar.2025.110656","DOIUrl":"10.1016/j.vetpar.2025.110656","url":null,"abstract":"<div><div>Ticks are globally important vectors of human and animal pathogens. This study characterized microbial communities in <em>Haemaphysalis longicornis</em> (from humid Zhangjiachuan County) and <em>Hyalomma dromedarii</em> (from arid Minqin County, Gansu Province, China) using metagenomic sequencing. We identified diverse bacteria and viruses including known pathogens (<em>Anaplasma phagocytophilum</em>, <em>Totivirus</em> spp., <em>Escherichia</em> spp.) and potentially novel agents (<em>Totivirus</em>-like and <em>Trachysalambria</em> curvirostris-associated sequences). These results highlight the role of these tick species in pathogen transmission across different environments and emphasize the need for region-specific surveillance. Our findings contribute to the understanding of tick-borne disease risks and inform strategies for targeted control.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110656"},"PeriodicalIF":2.2,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of qPCR assay targeting COWP conserved region for sensitive detection and quantification of Cryptosporidium infections","authors":"Hend Okasha ,&nbsp;Hadeer A. El-Kalamawy ,&nbsp;Ahmed R. Mashaal ,&nbsp;Eman S. El-Wakil","doi":"10.1016/j.vetpar.2025.110653","DOIUrl":"10.1016/j.vetpar.2025.110653","url":null,"abstract":"<div><h3>Background</h3><div><em>Cryptosporidium</em> is a significant food and waterborne protozoan parasite. Molecular diagnosis and quantification play a crucial role in diagnosing infections, as well as in understanding the transmission dynamics. The <em>Cryptosporidium</em> oocyst wall protein (COWP) gene serves as an appropriate target for such assays since it remains conserved uniquely among significant species.</div></div><div><h3>Objective</h3><div>The current research focused on developing and validating a sensitive and specific quantitative PCR (qPCR) test targeting a conserved domain of the COWP gene to detect and quantify various <em>Cryptosporidium</em> species.</div></div><div><h3>Methods</h3><div>Design of selective degenerate primers on a conserved domain in the major <em>Cryptosporidium</em> spp. COWP gene. A COWP DNA library was constructed using molecular cloning into pET-15b vector to be used as a precursor for a standard curve absolute quantification strategy.</div></div><div><h3>Results</h3><div>The designed primers successfully amplified a 311–317 bp product, with specificity tested using melt curve analysis. The slope of the standard curve was −3.279, efficiency of 100.8 %, and R² = 0.95 (p &lt; 0.0001) with LOD equals 9.55 × 10⁴ copies /µL.</div></div><div><h3>Conclusion</h3><div>A qPCR assay that is both sensitive and efficient was developed and validated. The method produces a reliable technique of absolute quantitation of <em>Cryptosporidium</em> DNA in samples of unknown quantity, from which infection rates may be estimated accurately.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110653"},"PeriodicalIF":2.2,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy assessment of a chewable tablet containing sarolaner, moxidectin, and pyrantel in the treatment of Angiostrongylus vasorum in dogs","authors":"Anne Lloyd ,&nbsp;Lore Van Mechelen ,&nbsp;Carleen Van Overloop ,&nbsp;Padraig Doherty ,&nbsp;Jakob L. Willesen ,&nbsp;Fabrizio Solari Basano ,&nbsp;Thomas Geurden","doi":"10.1016/j.vetpar.2025.110652","DOIUrl":"10.1016/j.vetpar.2025.110652","url":null,"abstract":"<div><div>Angiostrongylosis, caused by <em>Angiostrongylus vasorum</em>, is an important clinical disease in dogs in Europe with infection transmitted through infected snails and slugs which function as intermediate hosts. Previous studies demonstrated the preventive efficacy of a monthly administration of moxidectin in combination with sarolaner and pyrantel (Simparica Trio® chewable tablet (SMP); Zoetis). An experimental infection study and a field study were conducted to evaluate the efficacy and safety of SMP for the treatment of infections with adult stages of <em>A. vasorum</em> in dogs. The experimental study evaluated the efficacy of a single (n = 7 dogs) or repeated dose (n = 8 dogs) of SMP at the minimum recommended dose of 1.2 mg/kg sarolaner, 0.024 mg/kg moxidectin and 5 mg/kg pyrantel (as pamoate salt) administered as a chewable tablet. against an induced infection with 200 (±10) L3 <em>A. vasorum</em> at least 63 days prior to treatment on Day 0, compared to a placebo control product (n = 8 dogs). Efficacy was determined based on adult worm counts 56 or 57 days after treatment. The field study at veterinary <em>practices</em> in Italy and Denmark evaluated the efficacy and safety of SMP in client-owned dogs (n = 66). Advocate® Spot On (100 mg/mL (minimum 10 mg/kg) imidacloprid + 25 mg/mL (minimum 2.5 mg/kg) moxidectin from Elanco Animal Health, n = 37) was used as the control product. Treatment was administered on Day 0 and repeated on Day 30 if the dog was still positive for <em>A. vasorum</em> larvae. Faecal samples were collected and analyzed with the modified Baermann method in-between scheduled visits. In the experimental study the percentage reduction in geometric mean adult worm counts compared to the placebo-treated group was 98.3 % (<em>P</em> &lt; 0.0001) after a single dose of SMP and 99.6 % <em>(P</em> &lt; 0.0001) after two doses. In the field study, faecal larvae counts were reduced by 90 % or more in 90.9 % of the dogs in the SMP-treated group at Day 30 and in 98.5 % of dogs at Day 60. Compared to the pre-treatment faecal larvae counts a geometric mean percent reduction of 96.7 % was observed on Day 30 and 99.1 % on Day 60 in the SMP treated group. Both in the experimental and in the field study, SMP as a chewable tablet was well tolerated and resulted in a high efficacy against adult <em>A. vasorum</em> infections after a single treatment.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110652"},"PeriodicalIF":2.2,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}