Veterinary parasitology最新文献

{"title":"How can Mentha piperita oil affect Trichinella spiralis adults and larvae? In vitro and in vivo studies","authors":"Maha Mohamed Gomaa ,&nbsp;Inass Ibrahim Ahmed Zaki ,&nbsp;Hoda Adel Rashed","doi":"10.1016/j.vetpar.2025.110663","DOIUrl":"10.1016/j.vetpar.2025.110663","url":null,"abstract":"<div><div>The alarming concerns of the current anti-trichinosis therapeutics; low efficacy against encysted larvae, serious side effects, and resistance emergence necessitate seeking an alternative therapeutic option. The well-reported therapeutic potentials of peppermint essential oil (PEO) in multiple disorders involving parasitic infections inspired us to explore its anti-trichinosis capacity. This study assessed the <em>in-vitro</em> impact of PEO on <em>Trichinella spinalis</em> adult worms and muscle larvae at three concentrations (2.5 %, 5 %, 10 %) for two durations (4, 24 h). Additionally, its <em>in-vivo</em> efficacy against intestinal, muscle invasion, and late convalescent phases were evaluated in experimentally-infected mice. The phytochemical analysis of PEO revealed high content of menthone, menthol, total phenolic acid, and total flavonoids. It had potent antioxidant properties evidenced by monitoring DPPH scavenging activity (73.6 %) and NO serum levels. <em>In-vitro</em>, PEO had significantly-affected the survival and the cuticular ultrastructure of adults and muscle larvae, inducing the highest mortality rate and the most obvious damage at 10 % after 24 h. <em>In-vivo</em>, PEO treatment induced a significant reduction in parasite burden in the different stages of infection. PEO exhibited a superior activity over albendazole against invasive and encapsulated larvae. Histopathologically, PEO showed a marvelous amelioration of inflammatory infiltration and tissue damage in intestines and muscles and significantly reduced CD3⁺ immune-stained T-lymphocyte percentage. In conclusion, PEO revealed an obvious anti-trichinosis activity both <em>in-vitro</em> and <em>in-vivo</em>. The antioxidant capabilities and the anti-inflammatory possessions qualified it to be a promising, safe, therapeutic alternative for trichinosis. However, further studies should be conducted to standardize its dose and duration in human.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110663"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in silico evaluation of nematicidal lectin compounds from Artocarpus heterophyllus seeds targeting gastrointestinal helminths","authors":"Renata Cristina Borges da Silva Macedo ,&nbsp;Mirna Samara Dié Alves ,&nbsp;Francisco Chagas Barbalho Neto ,&nbsp;Pablo Leandro Filgueira Feitosa ,&nbsp;Mário Luan Silva de Medeiros ,&nbsp;Edgley Alves de Oliveira Paula ,&nbsp;Michele Dalvina Correia da Silva ,&nbsp;Ana Carla Diógenes Suassuna Bezerra","doi":"10.1016/j.vetpar.2025.110664","DOIUrl":"10.1016/j.vetpar.2025.110664","url":null,"abstract":"<div><div>Bioactive plant molecules are a promising alternative for chemically controlling gastrointestinal nematodes in small ruminants. Therefore, this study aimed to investigate the nematicidal activity of lectins from <em>Artocarpus heterophyllus</em> seeds against gastrointestinal nematodes of sheep and to evaluate their molecular targets using immunofluorescence and docking analyses. The three formulations (crude extract, protein fraction, and an isolated lectin mix) were subjected to the hemagglutinating activity assay and the egg hatching test (EHT). In addition, the isolated lectins in the mix were conjugated with fluorescein isothiocyanate (FITC) for an immunofluorescence evaluation of their helminth labeling potential. The crude extract and protein fraction were tested in a 100 % concentration, and the isolated lectin mix was tested in different concentrations, and all presented an inhibitory effect on egg hatching. EHT results showed that the crude extract and the protein fraction inhibited 95.5 % and 88.1 % of egg hatching, respectively. Meanwhile, the highest activity for the isolated lectin mix led to 24.4 % egg hatching inhibition. Moreover, immunofluorescence showed that FITC-conjugated lectins did not recognize embryo molecules inside the eggs but recognized molecular targets in the larvae. The molecular docking results suggest a significant interaction between the lectins ArtinM and Jacalin and membrane proteins of sheep gastrointestinal nematodes. Altogether, data show that the crude extract, protein fraction, and the isolated lectin mix inhibited egg hatching. In addition, isolated lectins interacted with biomolecules from first-stage larvae and showed <em>in silico</em> molecular interaction with membrane proteins of the evaluated nematodes. Finally, our data suggest that <em>A. heterophyllus</em> is a promising alternative for developing new antiparasitic drugs against sheep endoparasites.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110664"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it ovine cystic echinococcosis? Post-mortem inspection compared to laboratory analyses of lesions from a slaughterhouse in a hypo-endemic area of northern Italy","authors":"M. Rega ,&nbsp;M. Fozzer ,&nbsp;M. Genchi ,&nbsp;C. Cattabiani ,&nbsp;M. Semeraro ,&nbsp;M. Conter ,&nbsp;M. Di Pentima ,&nbsp;F. Armando ,&nbsp;G. Muresu Ibba ,&nbsp;L. Kramer ,&nbsp;A. Vismarra","doi":"10.1016/j.vetpar.2025.110671","DOIUrl":"10.1016/j.vetpar.2025.110671","url":null,"abstract":"<div><div>The larval stage of <em>Echinococcus granulosus</em> sensu lato causes cystic echinococcosis (CE), an important parasitic zoonotic disease infecting over an estimated one million people worldwide. Canids act as definitive hosts while livestock, mainly sheep, serve as intermediate hosts for the larval stage. Inspection at slaughter of intermediate hosts and destruction of affected organs play an important role in limiting parasite spread. The aim of the present study was to evaluate, over a 6-month period, the prevalence of CE in sheep in an hypo-endemic area for <em>E. granulosus,</em> as determined at slaughter and to verify diagnosis in the laboratory through gross morphological evaluation, histopathology and a multiplex end-point PCR. Sample collection was performed in an ovine slaughterhouse in northern Italy. Lungs and livers were examined visually and those with lesions compatible with CE according to the official veterinary control were then conferred to the laboratory. A total of 421 sheep were examined and CE was suspected in 101/421 (24 %). Following incision, however, only 36/101 showed lesions with morphological features of CE. Histology and end-point PCR were positive for <em>E. granulosus</em> in only 25/36 sheep, highlighting the CE prevalence at 5.9 % (25/421). Results suggest that current inspection at slaughter based on visualization alone may over-estimate CE prevalence in particular in hypo-endemic areas.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110671"},"PeriodicalIF":2.2,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A roadmap in detecting frequently reported bovine babesiosis: From blood smear to CRISPR","authors":"Binaka Prabashini Dasanayaka ,&nbsp;Sisira Lal Pathirana ,&nbsp;Ama Jayawardana ,&nbsp;Shiroma Mangalika Handunnetti ,&nbsp;Narmada Fernando ,&nbsp;Bandula Prasanna Galhena ,&nbsp;Sisira Jagathpriya Weerasena ,&nbsp;Andreas Nitsche ,&nbsp;Samantha Sudarshini Iddamaldeniya ,&nbsp;Ann-Kathrin Dietzsch","doi":"10.1016/j.vetpar.2025.110662","DOIUrl":"10.1016/j.vetpar.2025.110662","url":null,"abstract":"<div><div>Current diagnosis of <em>Babesia bovis</em> and <em>B. bigemina</em> relies on direct microscopy, nucleic acid detection, and serology. Light-microscopic analysis of Giemsa-stained smears still serves as the primary diagnostic modality at the point of care. However, carrier cattle, particularly those harbouring <em>B. bovis</em>, often carry parasites at levels far below the detection threshold, and such levels are sensitive only to DNA-based detection approaches. Early probe-hybridization techniques have been largely replaced by conventional Polymerase Chain Reaction (PCR), nested formats, and real-time quantitative PCR (qPCR)-which enable species-specific discrimination within closed-tube systems, thereby minimizing contamination risk. Species‑level identification is essential for clinical management, surveillance, and experimental studies. Duplex TaqMan qPCRs simultaneously distinguish <em>B. bovis</em> from <em>B. bigemina</em>, surpassing the analytical sensitivity offered by nested PCR (nPCR) methodologies. Reverse-line-blot (RLB) hybridisation broadens the diagnostic scope by concurrently detecting co-infections of <em>B. bovis</em> and B. <em>bigemina</em> and mixed haemoparasitic species in one workflow. However, the analytical sensitivity of RLB remains inferior to that of qPCR in detecting low-density and carrier-state infections of <em>B. bigemina</em>. RLB remains useful for retrospective genotyping when amplification is impractical or fails. Field-ready isothermal approaches have expanded the scope of molecular diagnostics beyond laboratory settings, facilitating field-level application and rapid on-site detection. Coupling Loop-mediated isothermal amplification (LAMP) with a lateral-flow dipstick (LFD) (LAMP-LFD) enables pen-side direct visual detection. Antibody tests are essential tools for herd-level surveillance. A recent chimeric Enzyme-Linked Immunosorbent Assay (ELISA) that combines three immunodominant <em>B. bovis</em> antigens broaden strain coverage and boosts diagnostic reliability. Recombinase polymerase amplification coupled to CRISPR-Cas12a cleavage has achieved single-target detection of <em>B. bigemina</em> from tick salivary-gland DNA, paving the way for innovative pen-side platforms, once cost and technical hurdles are overcome. Importantly, vector-based detection using appropriate tissues bearing an optimum level of ticks is species-dependent. <em>B. bigemina</em> sporozoites concentrate in nymph/adult salivary glands, whereas <em>B. bovis</em> sporozoites are produced mainly in larval salivary glands, so monitoring programs should stratify sampling accordingly.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110662"},"PeriodicalIF":2.2,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the efficacy of three trypanocidal drugs in controlling Trypanosoma evansi","authors":"Somaia Abouakkada ,&nbsp;Nadia Labn ,&nbsp;Safeya Henidy ,&nbsp;Samah S. Oda ,&nbsp;Mohamed E. Abd El-Hack ,&nbsp;Fatima S. Alaryani ,&nbsp;Aishah E. Albalawi ,&nbsp;Kamlah Ali Majrashi ,&nbsp;Amira Dewair","doi":"10.1016/j.vetpar.2025.110658","DOIUrl":"10.1016/j.vetpar.2025.110658","url":null,"abstract":"<div><div><em>Trypanosoma evansi</em> is an emerging zoonotic threat causing significant economic and health issues, especially in tropical and subtropical regions. Due to limited effective trypanocidal agents and increasing drug resistance, this investigation sought to evaluate the therapeutic efficacy of quinapyramine sulfate and chloride, melarsamine hydrochloride, and diminazene aceturate in 72 female Swiss albino mice. Each drug was administered at two dosages: quinapyramine (3 and 6 mg/kg), melarsamine hydrochloride (0.25 and 0.5 mg/kg), and diminazene aceturate (3.5 and 7 mg/kg). The efficacy was evaluated based on parasitaemia, relapse patterns, body weight changes, hematological and biochemical profiles, postmortem and histopathological findings. The results showed quinapyramine was ineffective at both dosages. However, doubling the therapeutic doses of melarsamine hydrochloride and diminazene aceturate enhanced their efficacy, although parasitaemia relapsed in all cases. Blood biochemical analyses revealed infection-induced anemia, leukocytosis, eosinophilia, and significant reductions in total protein, serum albumin, and globulin levels. Histopathological examination revealed evidence of degenerative pathology and tissue injury within the hepatic, splenic, cardiac, and renal parenchyma in all experimental groups with varying degrees. These findings suggest that melarsamine hydrochloride, at higher doses, may offer a more effective treatment for <em>T. evansi</em> infection, although relapse remains a challenge.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110658"},"PeriodicalIF":2.2,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and specific detection of Babesia vogeli using RPA/CRISPR-Cas12a: A feasible field-friendly diagnostic for canine babesiosis","authors":"Suphaporn Paenkaew ,&nbsp;Thippaporn Euppayo ,&nbsp;Rongdej Tungtrakanpoung ,&nbsp;Worapan Teapunvong ,&nbsp;Korakot Nganvongpanit ,&nbsp;Kittisak Buddhachat","doi":"10.1016/j.vetpar.2025.110660","DOIUrl":"10.1016/j.vetpar.2025.110660","url":null,"abstract":"<div><div><em>Babesia vogeli</em> is a protozoan parasite causing canine babesiosis, a tick-borne disease prevalent in tropical and subtropical regions. Its microscopic identification is challenging due to morphological similarity with other <em>Babesia</em> spp., and serological assays often yield inaccurate results. To address this issue, we developed a rapid, equipment-minimal diagnostic method combining recombinase polymerase amplification (RPA) with CRISPR/Cas12a (RPA/CRISPR-cas12a) for <em>B. vogeli</em>-specific detection. The RPA assay enables DNA amplification for both <em>B. vogeli</em> and <em>Hepatozoon canis</em>, while CRISPR/Cas12a using gRNA_Bab ensures specificity for <em>B. vogeli</em>, even in co-infections and other pathogens. This approach detects as few as 10⁵ copies within two hours for both readout platforms such as fluorescence and lateral flow dipstick (LFD). Forty canine blood samples were detected by RPA/CRISPR-cas12a to examine its performance. Results showed high concordance with qPCR-high resolution melting (HRM) (Cohen’s kappa: 0.93 for fluorescence, 0.81 for LFD), outperforming conventional PCR. The clinical sensitivity and specificity of RPA/CRISPR-cas12a were 100 % and 96.8 %, respectively and the concordance with qPCR-HRM was 97.5 %. RPA/CRISPR-cas12a for <em>Babesi</em>a spp. detection provided a simple, rapid, and accurate method, demonstrating promise for point-of-care diagnosis of canine babesiosis in resource-limited settings. This method showed high potential as a practical diagnostic tool in veterinary clinics, with accelerated surveillance to control outbreaks of <em>Babesia</em>-associated canine babesiosis.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110660"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of in vitro insecticide selection pressure to a susceptible strain of the sheep blowfly reveals different rates of resistance development and cross-resistance patterns","authors":"Andrew C. Kotze ,&nbsp;Neil H. Bagnall ,&nbsp;Angela P. Ruffell ,&nbsp;Nicholas M. Rolls","doi":"10.1016/j.vetpar.2025.110661","DOIUrl":"10.1016/j.vetpar.2025.110661","url":null,"abstract":"<div><div>The present study aimed to investigate the development of resistance to the pyrimidine derivative dicyclanil and the neonicotinoid imidacloprid in the sheep blowfly, <em>Lucilia cuprina</em>. We applied laboratory-based insecticide selection pressure to a susceptible strain and measured resistance patterns that emerged to all of the currently-available blowfly control chemicals. The dynamics of resistance emergence was quite different for the two compounds. Resistance to imidacloprid emerged after 4 rounds of selection, increased up to 9 rounds before then remaining relatively constant at almost 10-fold until the termination of the experiment after 15 rounds. Dicyclanil resistance did not emerge until the 13th round of selection, and then increased to approximately 3.5-fold at 15 rounds. This faster development of resistance to imidacloprid is in agreement with observations of resistance emergence in field populations of the blowfly over the last 25 years. The imidacloprid-selected strain also developed resistance towards dicyclanil (3.1-fold). Similarly, the dicyclanil-selected strain also showed resistance to imidacloprid (4.8-fold). This level of resistance to imidacloprid in the dicyclanil-selected strain (4.8-fold) was slightly higher than the level of resistance to dicyclanil itself (3.5-fold). The study provides insights into the dynamics of resistance emergence to different compounds in the sheep blowfly and describes cross-resistance patterns with important implications for chemical rotation strategies. In demonstrating the emergence of resistance to two chemical classes after exposure to single classes, this study illustrates the need to examine the potential impact of resistance mechanisms that can act across multiple insecticide classes when designing insecticide-use strategies for parasite control.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110661"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal parasites in dogs and their association with clinical manifestations of canine visceral leishmaniasis","authors":"Allan de Jesus Mendonça Severino ,&nbsp;Dharliton Soares Gomes ,&nbsp;André Tetzl Costa ,&nbsp;Maykelin Fuentes Zaldívar ,&nbsp;Nathália Moreira Teodoro de Oliveira ,&nbsp;Bianca Leal de Oliveira ,&nbsp;Paulo Ricardo Silva Coelho ,&nbsp;Felipe Bisaggio Pereira ,&nbsp;Paulo Ricardo de Oliveira Paes ,&nbsp;João Carlos França-Silva ,&nbsp;Rodolfo Cordeiro Giunchetti ,&nbsp;Stefan Michael Geiger","doi":"10.1016/j.vetpar.2025.110659","DOIUrl":"10.1016/j.vetpar.2025.110659","url":null,"abstract":"<div><div>Dogs are companion animals commonly present in spaces shared with humans. However, this close contact may facilitate the transmission of zoonoses, such as canine visceral leishmaniasis (CVL). Additionally, dogs are potential hosts of endoparasites that can influence the clinical manifestations of CVL due to immunological interference occurring in parasitic coinfections. This study aimed to investigate the occurrence of endoparasites in domestic dogs seropositive for CVL and associate their clinical manifestations with the presence of intestinal helminths. We analyzed 47 seropositive dogs (51.06 % males; 48.9 % females; 80.8 % of undefined breed) that underwent clinical examination, blood count, euthanasia, necropsy, and coproparasitological diagnosis. Dogs were grouped into three clinical categories: low (40.4 %), medium (34.0 %), and severe (25.5 %) based on a modified version of the LeishVet clinical staging system. Coproparasitological results indicated <em>Ancylostoma</em> sp. and <em>Giardia</em> sp. as the most prevalent. Based on necropsy, <em>Ancylostoma</em> sp. and <em>Dipylidium caninum</em> were the most common helminths. Dogs with CVL and intestinal helminths frequently had macrocytic hypochromic (p = 0.0455) and microcytic hypochromic (p = 0.01963) anemia. We also found that <em>Ancylostoma</em> sp. was associated with more severe CVL clinical staging (odds ratio [OR] = 3.3621; 95 % confidence interval [CI] = 2.6413–4.2796) and negatively associated with red blood cell counts (OR = 0.9278; CI = 0.8702–0.9893). We conclude that intestinal helminth infections in dogs with CVL may represent an important aggravating factor for the disease’s clinical progression.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110659"},"PeriodicalIF":2.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Green synthesis and larvicidal activity of ZnO nanoparticles against Lucilia sericata larvae using essential oil bio-reductants” [Vet. Parasitol. 340 (2025) 110633]","authors":"Hatice Tosyagülü Çeli̇k ,&nbsp;Nilüfer Vural ,&nbsp;Sibel Kaymak","doi":"10.1016/j.vetpar.2025.110655","DOIUrl":"10.1016/j.vetpar.2025.110655","url":null,"abstract":"","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110655"},"PeriodicalIF":2.2,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double antigen sandwich ELISA for detection of antibodies against Babesia bovis.","authors":"María Evangelina Primo,&nbsp;Beatriz Susana Valentini,&nbsp;Matilde Mazzucco Panizza,&nbsp;Nicolás Morel,&nbsp;Carolina Soledad Thompson","doi":"10.1016/j.vetpar.2025.110657","DOIUrl":"10.1016/j.vetpar.2025.110657","url":null,"abstract":"<div><div><em>Babesia bovis</em> is a hemoparasite responsible for bovine babesiosis, an economically important tickborne disease widespread in tropical and subtropical regions of the world. Serological tests are used in calves to evaluate the need for vaccination and the immunity achieved after vaccination. We developed, validated, and evaluated under field conditions a double-antigen sandwich ELISA (dasELISA) using a recombinant major surface antigen 2c (MSA2c) from a pathogenic and an attenuated <em>B. bovis</em> strain. The tests were validated using a panel of 1057 serum samples of known status (217 known-positive and 840 known-negative). Samples from 116 herds (n = 2771) were evaluated by indirect ELISA (iELISA) and dasELISA. Samples with disagreement between ELISAs (231 out of 594, 38.9 %) were analyzed by IFAT. The dasELISA cutoff was ≥ 52 %P, with a diagnostic sensitivity of 94.5 % (95 % CI, 90.5–97.1) and a diagnostic specificity of 96.4 % (95 % CI, 94.9–97.6). The area under the curve (AUC) was assessed to be 0.979, which corresponds to an excellent ability of the assay to discriminate truly infected from truly uninfected animals. Concordance among dasELISA and iELISA was 78.6 %, with a κ value of 0.567 (95 % CI = 0.537–0.598); meanwhile, 57 % and 43 % of the IFAT results agreed with dasELISA and iELISA, respectively. The dasELISA could be useful for large-scale detection of anti–<em>B. bovis</em> antibodies in calves pre- and post-vaccination and for seroepidemiologic investigations, given its appropriate sensitivity and specificity and the simplicity of production.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"342 ","pages":"Article 110657"},"PeriodicalIF":2.2,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145617322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}