Veterinary parasitology最新文献

{"title":"Development of a visual rapid detection method for aquatic animal-derived Trypanosoma via MIRA-LFD","authors":"Xue-Mei Yuan ,&nbsp;Xin-Zhi Weng ,&nbsp;Xia-Lian Bu ,&nbsp;Jing Chen ,&nbsp;Jing-Biao Jiao ,&nbsp;Xian-Qi Peng ,&nbsp;Wei-Song Ma ,&nbsp;Ling-Yun Lin ,&nbsp;Lei Huang ,&nbsp;Xiao-Yi Pan ,&nbsp;Hong-Xia Hu ,&nbsp;Ling-Wei Xu ,&nbsp;Jia-Yun Yao","doi":"10.1016/j.vetpar.2025.110636","DOIUrl":"10.1016/j.vetpar.2025.110636","url":null,"abstract":"<div><div><em>Trypanosoma</em> is a genus of flagellated protozoan parasites that commonly infect the blood of various vertebrates, including aquatic animals. Under natural conditions, these pathogens are vectored by leeches and may induce clinical manifestations such as anemia, edema, and appetite suppression in hosts. Prolonged infection can lead to severe weight loss, ultimately contributing to economic losses in aquaculture. In this study, we developed a rapid diagnostic assay combining Multienzyme isothermal rapid amplification (MIRA) with Lateral flow dipstick (LFD) visualization for detecting aquatic animal-derived <em>Trypanosoma</em>. Using Small Subunit Ribosomal DNA (SSU rDNA) gene as the detection target, specific primers and probes were designed. The optimal primer and probe combination were selected via primer screening. Through the optimization of reaction conditions and specificity and sensitivity testing, a method for detecting trypanosomes in aquatic animals was established using MIRA-LFD. The optimal reaction temperature for MIRA is 45℃. The reaction time is 15 min, and the result observation time is 5 min, resulting in a total duration of the entire detection process of 20 min. The specificity test results indicated that the method can specifically detect trypanosome DNA, with no cross-reactions observed with eight common parasites of aquatic animals (e.g., <em>Argulus</em> sp., <em>Cryptocaryon irritans</em>) or five host species (e.g., large yellow croaker, koi carp). The sensitivity test results demonstrated a detection limit of 8 pg/µL for trypanosome DNA (1000-fold higher than conventional PCR) and could detect as few as 1 trypanosome, compared to 100 trypanosomes required for conventional PCR. This method successfully detected trypanosome DNA from various tissue samples of large yellow croaker (<em>Larimichthys crocea</em>), including gill mucus, blood, and kidney tissue. In contrast, the PCR method only detected trypanosome DNA in blood samples. Clinical validation with 24 samples revealed 100 % detection rate for positive samples by MIRA-LFD, outperforming PCR’s 83.33 % (10/12) rate. The MIRA-LFD method developed in this study for detecting aquatic animal-derived trypanosomes is rapid, simple, highly sensitive, and specific. It requires no sophisticated or expensive equipment, making it ideal for basic laboratories and on-site testing.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110636"},"PeriodicalIF":2.2,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of marine leech Limnotrachelobdella okae infestation in miiuy croaker (Miichthys miiuy): An emerging threat to Chinese mariculture","authors":"Shunli Che ,&nbsp;Zequan Mo ,&nbsp;Hai Zhang ,&nbsp;Haoran Tang ,&nbsp;Xueming Dan ,&nbsp;Yanwei Li","doi":"10.1016/j.vetpar.2025.110637","DOIUrl":"10.1016/j.vetpar.2025.110637","url":null,"abstract":"<div><div>From January to April 2025, an unprecedented epizootic outbreak of <em>Limnotrachelobdella okae</em> infested <em>Miichthys miiuy</em> in China, reducing survival rates to 7.69 % and causing massive economic losses. Clinical manifestations included ulcerative lesions, gill filament pallor, and anorexia. The present study aimed to characterize this emerging parasitic threat and the involved pathogenic mechanisms. Leeches collected from infected fish were morphologically and phylogenetically identified. Although first documented to induce mortality in <em>M. miiuy</em>, <em>L. okae</em> exhibited broad host plasticity. Pathological impacts were assessed via hematological profiling and histopathology. The pathological effects of the infestation included a cascade of hemorrhagic anemia (40 % decrease in red blood cells and 39 % decrease in hemoglobin), systemic immunosuppression (69 % decrease in white blood cells), and anemia-induced multiple organ dysfunction (liver, spleen, head kidney, and trunk kidney). Furthermore, field data confirmed temperature (&gt;20 °C) as the dominant driver of epidemic termination (Pearson’s r = -0.717, <em>p</em> = 0.045). Environmental tolerance assays of <em>L. okae</em> demonstrated strict cryohalophilic dependence, with optimal viability at 5–15°C and 15–25 ‰ salinity, and complete mortality was observed at 25 °C. The present study identified <em>L. okae</em> as an emerging threat to mariculture diversification. The present findings expand the spectrum of high-risk parasites jeopardizing marine aquaculture. Elucidation of the behavioral ecology and pathogenesis of <em>L. okae</em> will advance targeted surveillance and mitigation strategies.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110637"},"PeriodicalIF":2.2,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145460022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Citrus aurantium extracts and limonene on Dipylidium caninum tegument: In vitro and in vivo study","authors":"Aliaa A. Abd-Elaziz ,&nbsp;Eman M. Abouelhassan ,&nbsp;Elham A. Elkhawass ,&nbsp;Obeid Shanab ,&nbsp;Waleed F. Khalil ,&nbsp;Abdelfattah M. Abdelfattah","doi":"10.1016/j.vetpar.2025.110635","DOIUrl":"10.1016/j.vetpar.2025.110635","url":null,"abstract":"<div><div>Dipylidiosis is a common zoonotic infection of dogs caused by <em>Dipylidium caninum</em>. Infected dogs were treated using ethanolic extracts of the peel and seed of <em>Citrus aurantium</em>, as well as its main active compound (limonene). <em>In vitro</em>, peel and seed extracts (12.5, 25, 50 mg/ml) as well as limonene (30, 60, 120 µl/ml) induced worm immobility. Complete mortality of worms (100 %) was achieved 100 % mortality after 4 h for peel extracts and limonene and 6 h for seed extracts. Histological analysis and scanning electron microscopy showed significant structural damage in treated worms, including peeling, vesiculation, ulceration, fissures, and tegumental distortion. <em>In vivo</em>, dogs treated with peel extract at 150 and 300 mg/kg achieved high efficacy rates of 72.33 % and 92 %, respectively, and reached 100 % efficacy, comparable to that of praziquantel, by the end of the experiment. The extract's effectiveness was dose- and time-dependent. In conclusion, these findings suggest that <em>C. aurantium</em> peel extract provides a safe, sustainable, and effective natural alternative to synthetic drugs for treating <em>D. caninum</em> infections in dogs.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110635"},"PeriodicalIF":2.2,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145374258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains","authors":"Magalí Nicole Valenzano ,&nbsp;Liliana Alvarez ,&nbsp;Beatriz Valentini ,&nbsp;Silvina Wilkowsky","doi":"10.1016/j.vetpar.2025.110634","DOIUrl":"10.1016/j.vetpar.2025.110634","url":null,"abstract":"<div><div>Babesia bovis is a tick-borne parasite of major economic impact in the livestock industry. Control strategies rely mainly on the use of acaricides and live attenuated vaccines. Comparative genomic analyses have shown no major differences between virulent and attenuated B. bovis strains, suggesting that studies on the host’s differential response may represent a key step toward clarifying the basis of disease severity and vaccine efficacy. In this study, we analyzed by RNA-seq the differential gene expression in bovine monocyte-derived macrophages after phagocytosis of erythrocytes infected with either the virulent <em>B. bovis</em> S2P strain or the attenuated R1A one. The results revealed a common transcriptional core response of several cellular processes largely centered on lymphocyte related functions, cytokine regulation, and adaptive immune signaling. In turn, the two strains elicited contrasting responses in bovine macrophages, where the virulent strain induced the enrichment of lymphocyte- and antiviral-related pathways, and the attenuated strain led to a stronger pro-inflammatory, chemotactic, and extracellular matrix remodeling signatures. Taken together, these data improved our understanding of the early transcriptional events that develop in macrophages in response to the phagocytosis of red blood cells containing contrasting <em>B. bovis</em> strains. This large dataset could be evaluated in further studies to better characterize the pathogenic mechanisms of bovine babesiosis and to identify targets for host-directed therapeutic strategies.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"341 ","pages":"Article 110634"},"PeriodicalIF":2.2,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis and larvicidal activity of ZnO nanoparticles against Lucilia sericata larvae using essential oil bio-reductants","authors":"Hatice Tosyagülü Çeli̇k ,&nbsp;Nilüfer Vural ,&nbsp;Sibel Kaymak","doi":"10.1016/j.vetpar.2025.110633","DOIUrl":"10.1016/j.vetpar.2025.110633","url":null,"abstract":"<div><div><em>Lucilia sericata</em>, a dipteran fly, causes myiasis by infesting living or necrotic tissues and transmits mycobacterial infections to livestock. Its potential resistance to common insecticides underscores the need for integrated pest management strategies and highlights the importance of biopesticides. In this context, larvicidal effects of <em>Citrus sinensis</em>, <em>Cymbopogon flexuosus</em>, <em>Achillea millefolium</em>, <em>Origanum onites</em>, <em>Salvia fruticosa</em>, and <em>Mentha piperita</em> essential oils and biocapsulated ZnO nanoparticles were investigated to control <em>Lucilia sericata</em> vectors. Synthesized ZnO nanoparticles were characterized using UV-Vis spectrophotometry, SEM and EDX techniques. By analytical characterization, sizes of nanoparticles loaded with <em>Cymbopogon flexuosus</em> and <em>Citrus sinensis</em> essential oils were 112 and 230, respectively, and SEM analysis confirmed monoclinic and hexagonal crystallites. The larvicidal effects of oils and nanoparticles were determined at various concentrations (0.5, 1, 5, 10 and 20 µg/ml) and all oils except <em>Origanum onites</em> showed high larvicidal effects and a linear increase in % mortality was observed with increasing concentration. To understand molecular mechanisms of larvicidal effect of oils on <em>Lucilia sericata</em> larvae, in silico analyses revealed that the highest antagonistic activity against the cytochrome P450 6A1-like protein of the species homology modeled was mediated by carvone and vertonal compounds, and predictions of ADMET properties revealed the properties of volatile bioactive components as a low toxicity alternative to temefos, a currently used larvicide. This research demonstrates the usability of the six essential oils and biosynthesized nanoparticles as highly effective, natural fly vectors and control pesticides.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110633"},"PeriodicalIF":2.2,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the viability of Fasciola hepatica eggs collected from fecal and gallbladder samples in experimentally infected sheep","authors":"S. Ortega-Vargas ,&nbsp;I. Cruz-Mendoza ,&nbsp;J.A. Figueroa-Castillo ,&nbsp;A.V. Balderas-Loaeza ,&nbsp;A. Villa-Mancera ,&nbsp;F. Utrera-Quintana ,&nbsp;H. Quiroz-Romero","doi":"10.1016/j.vetpar.2025.110631","DOIUrl":"10.1016/j.vetpar.2025.110631","url":null,"abstract":"<div><div>The objective of this study was to determine and compare the viability of <em>Fasciola hepatica</em> eggs collected from fecal and gallbladder samples through the egg hatching test of experimentally infected sheep. The biological samples used come from a previously reported sheep vaccination trial. Twenty-five sheep free of fasciolosis and housed in pens with cement floors were used. The sheep were grouped into five groups (G1–5, n = 5 sheep per group). Groups G1(100 ug), G2 (200 ug), G3 (400 ug) were immunized with the chimeric protein (rFhLAP-CL1) and, groups G4 and G5 were the adjuvant and infection controls, respectively. All sheep were orally infected with 200 metacercariae. The experimental period was 24 weeks. <em>Fasciola hepatica</em> eggs were collected every 15 days from fecal samples from weeks 14–24 of the experiment, and from the gallbladder after euthanized. The viability of <em>F. hepatica</em> eggs was determined through an egg hatching assay (EHA). Eggs that had developed miracidium and hatched were considered viable eggs. The viability range of <em>F. hepatica</em> eggs from gallbladder was 22–59.6 % and of fecal samples was 67–88 %. The viability of <em>F. hepatica</em> eggs from fecal samples was higher (&gt;90 %) than that of eggs from the gallbladder (&lt;60 %). In the vaccination trial, the viability of <em>F. hepatica</em> eggs in the G3 group was significantly different compared to the control group, in fecal samples (p &lt; 0.001) and in the gallbladder (p &lt; 0.05). Higher viability of <em>F. hepatica</em> eggs was observed in fecal samples than in gallbladder samples. Fecal samples are suggested for determining the viability of trematode eggs in vaccination trials or evaluation of fasciolicides.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110631"},"PeriodicalIF":2.2,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chi-miR-146a promotes the propagation of Neospora caninum by targeting the TGF-β1/SMAD2 pathway","authors":"Jin-Ming Chen,&nbsp;Jing-Yu Li,&nbsp;Jia-Yi Jin,&nbsp;Xiao-Qi Bao,&nbsp;De-Liang Tao,&nbsp;Xiao-Yun Zhao,&nbsp;Jun-Ke Song,&nbsp;Xin Yang,&nbsp;Ying-Ying Fan,&nbsp;Guang-Hui Zhao","doi":"10.1016/j.vetpar.2025.110632","DOIUrl":"10.1016/j.vetpar.2025.110632","url":null,"abstract":"<div><div><em>Neospora caninum</em> is known to induce abortions in various domestic animals (especially cattle and sheep), leading to substantial economic losses in the livestock industry. However, effective strategies for controlling <em>N. caninum</em> infection remains elusive. The precise mechanisms underlying the interaction between <em>N. caninum</em> and its host are not fully elucidated. Our previous study showed an upregulated microRNA (miRNA), Chi-miR-146a, promoted the propagation of <em>N. caninum</em> tachyzoites in caprine endometrial epithelial cells (EECs). The present study investigated its regulatory function during <em>in vitro</em> infection of <em>N. caninum</em>. The expression of Chi-miR-146a was upregulated from 12 h post infection (hpi) to 48 hpi. One of target genes for Chi-miR-146a, <em>tgfb1</em>, was identified and the mRNA and protein levels were downregulated at 24 hpi and 48 hpi. Overexpression of <em>tgfb1</em> suppressed the propagation of <em>N. caninum</em> tachyzoites in caprine EECs. Further analysis indicated that Chi-miR-146a enhanced <em>in vitro</em> propagation of <em>N. caninum</em> propagation through suppressing the activation of the TGF-β1/SMAD2 signaling pathway. The findings in this study offer a novel theoretical framework for advancing our understanding on mechanisms of intracellular survival of <em>N. caninum</em> and devising preventive strategies against this pathogen.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110632"},"PeriodicalIF":2.2,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthelmintic efficacy and sublethal effects of trichlorfon in pirarucu (Arapaima gigas) naturally infected with Dawestrema cycloancistrium","authors":"Jeffesson de Oliveira-Lima ,&nbsp;Patricia Oliveira Maciel-Honda ,&nbsp;Jayne Barros de Aguiar Silva ,&nbsp;Liana Bezerra Dias de Lima ,&nbsp;Sandro Estevan Moron","doi":"10.1016/j.vetpar.2025.110630","DOIUrl":"10.1016/j.vetpar.2025.110630","url":null,"abstract":"<div><div>Trichlorfon is an organophosphate commonly used to control fish ectoparasites due to its cholinesterase-inhibiting action. Due to recurring issues with monogeneans in pirarucu, this active ingredient has been frequently used in this aquaculture system. The present study aimed to simultaneously evaluate the antiparasitic efficacy and physiological effects of trichlorfon in <em>A. gigas</em> juvenile naturally infected with monogenean <em>Dawestrema cycloancistrium</em>. In the <em>in vitro</em> assay, parasitized gill arches were exposed to trichlorfon (0.1 mg/L - 10 g/L) under controlled conditions, with parasite motility analyzed every 30 min. A 100 % mortality rate was observed after 30 min at 10 g/L and after 3 h at 10 mg/L; concentrations ≤ 1 mg/L caused only a reduction in parasite motility. In the <em>in vivo</em> trial, 96 juveniles were separated into four groups: control, 10 g/L for 30 min, 10 mg/L for 3 h and 10 mg/L for 96 h. The 10 mg/L for 96 h treatment showed the highest efficacy (96.9 %), significantly surpassing the others (49.7 % and 42.3 %; p &lt; 0.05). However, this prolonged exposure induced hypochromic anemia, leukopenia, reduction in ALT and AST levels, and hyperglycemia. It is concluded that trichlorfon short-bath protocols, such as 10 g/L for 30 min or 10 mg/L for 3 h did not cause significant physiological alterations but resulted in only partial efficacy (∼50 %). Prolonged exposures at 10 mg/L for 96 h should not be recommended for <em>A. gigas</em> due to their hematotoxic and hepatotoxic effects.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110630"},"PeriodicalIF":2.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of plant-based remedies for Varroa destructor control in honey bee colonies: A preliminary study","authors":"Ľubomír Korený,&nbsp;Michaela Korená Hillayová,&nbsp;Jaroslav Škvarenina","doi":"10.1016/j.vetpar.2025.110629","DOIUrl":"10.1016/j.vetpar.2025.110629","url":null,"abstract":"<div><div>The Western honey bee (<em>Apis mellifera</em> L.) plays a crucial role in global ecosystems and agriculture, yet the ectoparasitic mite <em>Varroa destructor</em> remains a primary cause of severe varroosis and significant colony morbidity and mortality. Current reliance on synthetic acaricides often leads to the development of mite resistance and raises concerns regarding chemical residues in hive products. This has created an urgent need for innovative and effective veterinary strategies for parasite control. This study investigated the efficacy of traditional plant-based remedies as an alternative treatment for <em>V. destructor</em> infestations in honey bee colonies. Herbal decoctions from medicinal plants (e.g., nettle, clove, linden, peppermint, lavender, sage, dandelion), known for their anti-parasitic properties, were applied to bee colonies via aerosol. <em>V. destructor</em> infestation levels were monitored monthly using a non-destructive CO₂-induced narcosis method. Statistical analysis, specifically a Linear Mixed Model (LMM), revealed a significant decrease in mean <em>Varroa</em> infestation from 1.8 % in 2021–1.1 % in 2023 across the experimental colonies (<em>p</em> &lt; 0.000001 for year effect). Furthermore, the LMM confirmed significant differences in efficacy among the tested decoctions (<em>p</em> &lt; 0.000001 for Decoction label effect), with specific extracts (DIII and DV) demonstrating superior performance. These findings highlight a validated, ecologically derived phytotherapeutic approach for <em>Varroa</em> management, demonstrating its potential to significantly reduce mite populations over multiple years without adverse effects on honey bee health. This research provides valuable insights into natural alternatives for parasitic mite control in domesticated animals like honey bees.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110629"},"PeriodicalIF":2.2,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145309398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart farming with AI: Enhancing anemia detection in small ruminants","authors":"Sadia Farhana","doi":"10.1016/j.vetpar.2025.110627","DOIUrl":"10.1016/j.vetpar.2025.110627","url":null,"abstract":"","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110627"},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}