Veterinary parasitology最新文献

{"title":"Evaluation of the efficacy of disinfectants and disinfection methods against Ascaris suum eggs","authors":"Ruisi Zhang,&nbsp;Maolin Lu,&nbsp;Hongyan Yu,&nbsp;Tianlu Liu,&nbsp;Zhiying Cui,&nbsp;Yuling Yang,&nbsp;Mengchao Zhou,&nbsp;Yaxian Lu,&nbsp;Hang Tian,&nbsp;Lihong Tian,&nbsp;Lei Han,&nbsp;Zhijun Hou","doi":"10.1016/j.vetpar.2024.110333","DOIUrl":"10.1016/j.vetpar.2024.110333","url":null,"abstract":"<div><div><em>Ascaris</em> is highly adaptable, allowing its offspring to thrive in various conditions and posing significant health risks widely among animal populations. Most studies regarding the efficacy of disinfectants against <em>Ascaris</em> eggs in animal houses have been limited and lack a systematic and comprehensive evaluation. Currently, <em>Ascaris suum</em> is one of the most extensively studied helminths in the context of parasitology. Here, 8 disinfectants, UV radiation and quicklime were used to treat <em>A</em>. <em>suum</em> eggs, which were subsequently incubated at a room temperature of 22–25ºC for 15 days. The inactivation rate of <em>A. suum</em> eggs (expressed as a percentage) was measured to assess the efficacy of disinfectants, UV radiation, and quicklime in inactivating <em>A. suum</em> eggs. The results indicated that 1 %-10 % povidone iodine, 5 %-25 % ammonia solution, 0.5–2 % chlorine dioxide, 75 % ethanol and formalin in long-term (15 days), as well as the 5 % and 10 % povidone iodine, 25 % ammonia solution and UV irradiation in short-term (30–120 min) completely inhibited the normal development of <em>A. suum</em> eggs up to L2 stage. In conclusion, 75 % ethanol, povidone iodine, chlorine dioxide, ammonia solution, formalin, and UV irradiation are effective in inactivating <em>A. suum</em> eggs for dual disinfection of parasites and microorganisms. Among them, povidone iodine and UV irradiation are relatively efficient and environmentally friendly disinfection methods, and chlorine dioxide, a relatively harmless and broad-spectrum disinfectant, is an alternative choice for <em>A. suum</em> eggs elimination.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110333"},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis of extracellular vesicles and extracellular vesicle-depleted excretory-secretory products of Toxocara canis and Toxocara cati larval cultures","authors":"Timothy K. Wu ,&nbsp;Qin Fu ,&nbsp;Janice L. Liotta ,&nbsp;Dwight D. Bowman","doi":"10.1016/j.vetpar.2024.110331","DOIUrl":"10.1016/j.vetpar.2024.110331","url":null,"abstract":"<div><div><em>Toxocara canis</em> and <em>Toxocara cati</em> are parasitic nematodes in the order Ascaridida, which inhabit the small intestines of dogs and cats, respectively, as adults. Although often nonpathogenic as adults, nematodes within this genus are capable of causing widespread disease throughout the host while in a larval stage, during which time larvae migrate throughout the body in a process termed larva migrans. Larvae are also capable of surviving within host tissues in an encysted arrested stage, without immune clearance by the host. The ability of larvae to survive within host tissues during migration and encystment may be attributed to immunomodulatory molecules released by the excretory cells of larvae in excretory-secretory (ES) products. ES products of parasites contain a variety of molecules, including proteins, lipids, and extracellular vesicles (EVs). <em>Toxocara</em> excretory-secretory (TES) products have been studied to some degree, with proteomic analysis of TES proteins described previously; however, investigation of the EVs within TES is lacking, despite the suggested role for these molecules in host interaction and potential immunomodulation. To further characterize the protein cargo within EVs in TES, EVs were isolated from larval cultures of <em>T. canis</em> and <em>T. cati</em> via ultrafiltration, with concurrent collection of EV-depleted TES filtrate for additional study. Isolated EVs and EV-depleted TES from both <em>T. canis</em> and <em>T. cati</em> were submitted for proteomic analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteomic identification results revealed 140 proteins across all samples, with 16 shared by all samples, and 76 total proteins shared between <em>T. canis</em> and <em>T. cati</em>, present within EVs and EV-depleted TES. There were 17 proteins shared exclusively by EV samples, and 15 were shared exclusively between EV-depleted TES samples. Many shared proteins were associated with the host immune response. Several proteins were specific to either <em>T. canis</em> or <em>T. cati</em>, highlighting the potential use of these proteins as diagnostic tools in the differentiation of etiologic agents in cases of toxocariasis. The results of this study build upon previously reported proteomic evaluations of TES, contributing new information in regards to newly identified proteins, EV protein cargo within TES, and potential immunomodulatory functions of these proteins.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110331"},"PeriodicalIF":2.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global and regional prediction of heterakidosis population prevalence in extensive backyard chickens in low-income and middle-income countries","authors":"Violeta Muñoz-Gómez ,&nbsp;Paul R. Torgerson","doi":"10.1016/j.vetpar.2024.110329","DOIUrl":"10.1016/j.vetpar.2024.110329","url":null,"abstract":"<div><div>Extensive backyard chickens are one of the most common production systems in low-income and middle-income countries (LMICs). In this production system, chickens are exposed to infectious forms of parasites as a result of the outdoor access and scavenging behaviour. <em>Heterakis gallinarum</em> is one of the most common nematode parasites present in the environment, and estimating its global and regional prevalence is essential for attributing the economic losses in extensive backyard chickens. The objective of this study is to predict the prevalence of heterakidosis in extensive backyard chickens at global and regional levels in LMICs using regression imputation methods. A binomial random effect model was developed using empirical data on heterakidosis prevalence and climatic factors as main predictors. Prevalence data were then imputed in all regions based on the regression model. Global and country prevalence were estimated based on regional predictions and their beta distributions. Minimum precipitation, minimum temperature and maximum temperature were selected as significant predictors. The population prevalence of heterakidosis was 0.24 (0.19–0.29). Countries with continental and dry climates had a higher mean prevalence, whereas countries with tropical climates had a lower mean prevalence of heterakidosis. As more empirical data on heterakidosis prevalence become available, this model and predictions should be redefined and updated to capture a more representative association and increase the accuracy of the predictions. The results of this study can be used to attribute the economic losses of extensive backyard chickens, taking into account a holistic approach as promoted by the GBADs programme and therefore, to identify which diseases are more costly to backyard farmers. Furthermore, results can be also served as a proxy for the risk of histomoniasis in extensive backyard chickens.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110329"},"PeriodicalIF":2.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levonorgestrel enhanced Toxoplasma gondii infection risk via progesterone receptor upregulation","authors":"Shenghui Wang,&nbsp;Tiancong Sun,&nbsp;Yan Huang,&nbsp;Rongsheng Mi,&nbsp;Yan Zhang,&nbsp;Haiyan Gong,&nbsp;Zhaoguo Chen","doi":"10.1016/j.vetpar.2024.110330","DOIUrl":"10.1016/j.vetpar.2024.110330","url":null,"abstract":"<div><div>The numerous stray cats and dogs worldwide pose a huge burden on local governments, keeping residents safe and healthy, and maintaining clean cities with good traffic situations. To effectively control the populations of these stray animals, while considering animal welfare, researchers have suggested the use of levonorgestrel (LNG) as a contraceptive method. However, the potential side effects of LNG on these animals need to be evaluated before widespread application. Since dogs and cats play important roles in the transmission of <em>Toxoplasma gondii</em> as intermediate and definitive hosts, respectively, it is critical to assess the safety of LNG from the perspective of its effect on <em>Toxoplasma</em> susceptibility. In this study, the susceptibility of mice to <em>T. gondii</em> infection was investigated <em>in vivo</em> and <em>in vitro</em> with the presence of LNG. As a result, LNG increased the risk of infection, which was probably associated with the downregulation of IFN-γ levels, but not with the alteration of host sex hormone levels. Concurrently, the presence of LNG enhanced the expression of its ligand, the progesterone receptor (PGR), on the host cells. The promotional effect of LNG on <em>T. gondii</em> infection was attenuated when the <em>PGR</em> gene was knocked down. This investigation represents the inaugural study of LNG's side effects on <em>T. gondii</em> infection in mice, underscoring the significance of choosing or developing suitable contraceptive drugs for stray animals.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110330"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First documentation of dose response of Amblyomma cf. parvum population infesting dogs to amitraz, coumaphos, and cypermethrin","authors":"Roger I. Rodríguez-Vivas ,&nbsp;Melina M. Ojeda-Chi ,&nbsp;Iris Trinidad-Martinez ,&nbsp;Gabriela J. Flota-Burgos ,&nbsp;Nadia F. Ojeda-Robertos","doi":"10.1016/j.vetpar.2024.110328","DOIUrl":"10.1016/j.vetpar.2024.110328","url":null,"abstract":"<div><div><em>Amblyomma</em> cf. <em>parvum</em> (Ixodida: Ixodidae) is an ectoparasite of domestic animals and wildlife on the American continent. There is little information on the efficacy of acaricides for the control of this tick species in domestic animals. Here, we determined the toxicological response of <em>A</em>. cf. <em>parvum</em> to amitraz, coumaphos, and cypermethrin. Engorged females of <em>A</em>. cf. <em>parvum</em> were collected (in two periods, eight months apart) from dogs in Yucatan, Mexico. The larval immersion test was used on the progeny of adult female ticks to test for amitraz susceptibility, and the larval package test was used to test for coumaphos and cypermethrin susceptibility. Dose–mortality regressions, lethal concentrations (LC), 95 % confidence intervals (CI95 %), and slopes were estimated by probit analysis. The lethal concentrations to kill 50 % of the tick population for amitraz, coumaphos, and cypermethrin in the first period were 1 ppm (CI95 %: 1–3 ppm), 10 ppm (CI95 %: 9–11 ppm), and 10 ppm (CI95 %: 9–10 ppm), respectively; meanwhile in the second period were 2 ppm (CI95 %: 1–3 ppm), 8 ppm (CI95 %: 6–10 ppm), and 9 ppm (CI95 %: 8–10 ppm), respectively. In conclusion, amitraz, coumaphos, and cypermethrin demonstrated high larvicidal efficacy in <em>in vitro</em> bioassays against <em>A</em>. cf. <em>parvum</em> populations infesting dogs.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110328"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface hydrophobicity mechanism of poultry red mite, Dermanyssus gallinae (Acari: Dermanyssidae), gives novel meaning to chemical control","authors":"Bohan Wang,&nbsp;Jiali Meng,&nbsp;Xiaoxiao Qi,&nbsp;Penglong Wang,&nbsp;Qi Liu,&nbsp;Lifang Wang,&nbsp;Weiwei Sun,&nbsp;Baoliang Pan","doi":"10.1016/j.vetpar.2024.110327","DOIUrl":"10.1016/j.vetpar.2024.110327","url":null,"abstract":"<div><div>Surface hydrophobicity of organisms provides biological self-protection. The hydrophobicity of pest surface, acting as a main obstacle for the pest control, can lead to low utilization and high loss of pesticides. <em>Dermanyssus gallinae</em> is a notorious pest in egg-laying hens, whose control primarily depends on acaricide spraying, while its surface hydrophobicity and potential influence on pesticide effectiveness are not clear. In the present study, the contact angle measurements revealed that the surface of <em>D. gallinae</em> was hydrophobic. Analysis using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the surface microstructures of <em>D. gallinae</em> consist of cuticular folds, with a lipid-rich outermost layer of the cuticle. Based on gas chromatography-mass spectrometry (GC-MS) and gas chromatography (GC), it was found that the major compositions of cuticular lipids were fatty acids and n-alkanes. Modifying the chemical compositions and microstructures of the <em>D. gallinae</em> surface resulted in a reduction in surface hydrophobicity and an increase in the permeation of Rhodamine B through the cuticle. This observation suggested that the chemical compositions and microstructures were pivotal in determining surface hydrophobicity, hindering compound penetration into the cuticle. Finally, it was found improving the wettability of pesticide solution by adding surfactants could overcome the surface hydrophobicity and enhance the efficacy of pesticide against the mites. This study sheds light on the surface hydrophobicity mechanism of <em>D. gallinae</em> and provides a novel strategy to improve the efficacy of acaricides against the mites.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110327"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review on buparvaquone resistance associated with non-synonymous mutation in drug binding genes site of Theileria annulate","authors":"Muhammad Rashid ,&nbsp;Muhammad Hassan Hayat ,&nbsp;Naveed Zahra ,&nbsp;Mian Sayed Khan ,&nbsp;Suleman ,&nbsp;Muhammad Nadeem ,&nbsp;Tauseef ur Rehman ,&nbsp;Muhammad Ehsan ,&nbsp;Muhammad Irfan Malik ,&nbsp;Muhammad Kashif Obaid ,&nbsp;Amir Bakhsh ,&nbsp;Mohamed Aziz Darghouth ,&nbsp;Qiaoyun Ren","doi":"10.1016/j.vetpar.2024.110321","DOIUrl":"10.1016/j.vetpar.2024.110321","url":null,"abstract":"<div><div><em>Theileria annulata</em> (<em>T. annulata</em>) is intra-erythrocytic protozoan parasite which is more prevalent in tropical and sub-tropical countries. It has a significant economic impact on the productivity of the dairy industry, and buparvaquone is used to treat infected animals in the prevalent regions of the world. Systematically, buparvaquone targets the cyto-b gene to break the electron transport chain (ETC) and <em>Theileria annulata</em> peptidyl-prolyl isomerase 1 (TaPIN1) gene to destabilize transcription factor JUN (c-JUN) to inhibit proliferation of infected cells, which ultimately leads to the death of <em>T. annulata</em>. The reported studies on drug resistance is due to inappropriate drug application, evolutionary characteristics of the cytochrome b (cyto-b) gene and oncogenic signaling pathways gene (TaPIN1) make the parasite resistant against buparvaquone. Hence, this systematic review was designed to find out non-synonymous mutation in genes (cyto-b and TaPIN1) responsible for drug resistance reported from Tunisia, Turkey, Egypt, Sudan, Iran, Pakistan, China and Germany with reference to the <em>T. annulata</em> Ankara strain of cyto-b (accession no. XM_949625.1) and TaPIN1 (accession no. TA18945) wild type genes. Non-synonymous point mutations were found in cyto-b (Q₀₁ at 130–148 and Q₀₂ at 253–262 regions) and TaPIN1 (A53P and A53T) genes. These point mutations are responsible for developing buparvaquone resistance against <em>T. annulata</em> infection. These genes can be used as biomarkers for the identification of drug resistance in any endemic area. To avoid the complication of drug resistance, development of genetically resistant cattle breeds, potent vaccines and anti-theilerial drugs (Trifloxystrobin and anti-cancerous) are currently required to control proliferating economically important <em>T. annulata</em> parasites.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110321"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-resistance patterns in field strains of the sheep blowfly following laboratory-based selection pressure with dicyclanil or imidacloprid","authors":"Andrew C. Kotze ,&nbsp;Angela P. Ruffell ,&nbsp;Nicholas M. Rolls","doi":"10.1016/j.vetpar.2024.110326","DOIUrl":"10.1016/j.vetpar.2024.110326","url":null,"abstract":"<div><div>Control of the sheep blowfly relies largely on the use of insecticides applied prophylactically in advance of expected fly activity. However, the blowfly has shown an ability to develop resistance to some of these insecticides. Recent reports of the co-occurrence of resistance to both dicyclanil and imidacloprid in <em>in vitro</em> bioassays with field-collected fly strains has raised the possibility that the two resistances may represent cross-resistance linked by a common mechanism. We investigated this by imposing insecticide selection pressure on larvae of two insecticide-resistant field strains over a number of generations using either dicyclanil or imidacloprid and then measuring changes in sensitivity to both the selecting chemical and the alternate chemical. Larvae selected over six generations with each chemical showed significant increases in resistance to the selecting chemical: resistance ratios at the IC₅₀ 5.5 - 8.1-fold higher for dicyclanil, and 3.1 - 3.8-fold for imidacloprid. The larvae also showed significant increases in levels of resistance towards the alternate chemical: resistance ratios 2.6 - 3.1-fold higher towards dicyclanil following selection with imidacloprid, and 2.2 - 3.2-fold higher towards imidacloprid following selection with dicyclanil. The increases in resistance to both chemicals after exposure to either suggests a common mechanism of resistance, at least in our laboratory-selected populations. Assays with the cytochrome P450 inhibitor aminobenzotriazole showed that this synergist was able to remove the increased resistance to both compounds in strains selected with either compound, suggesting that cytochrome P450 is responsible for the resistance observed to both chemicals. Our results confirm that cross-resistance exists between dicyclanil and imidacloprid in the sheep blowfly, and hence the two compounds should be considered as related entities in insecticide rotation strategies for flystrike control.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110326"},"PeriodicalIF":2.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cats and dogs as hosts of Blastocystis – What is the evidence?","authors":"Supaluk Popruk ,&nbsp;Khuanchai Koompapong ,&nbsp;Aongart Mahittikorn ,&nbsp;Lee O.’Brien Andersen ,&nbsp;Christen Rune Stensvold","doi":"10.1016/j.vetpar.2024.110325","DOIUrl":"10.1016/j.vetpar.2024.110325","url":null,"abstract":"<div><div><em>Blastocystis</em> is one of the most common intestinal parasites observed in human and non-human hosts. Recent meta-analyses have indicated a potential role for pets such as dogs and cats as reservoir hosts of <em>Blastocystis</em>, but the data underpinning this hypothesis are of mixed quality. Reviewing data for 45,894 samples tested for <em>Blastocystis</em> by DNA-based methods and 11,908 subtype observations, a model was developed for calculating indices that could be used for evaluating individual species as natural hosts of <em>Blastocystis</em>, based on weighted products of positivity rates and subtype distributions. Data from cats and dogs were analysed, using other well-sampled hosts (pig, cattle, sheep, goat, and human) as references. Data from cats and dogs meeting the inclusion criteria were entered into the model. The overall positivity rates for pigs, cattle, sheep, goats, humans, dogs, and cats were 40 %, 40 %, 35 %, 28 %, 25 %, 6 %, and 5 %, respectively, with statistically significant lower positivity rates in cats and dogs (<em>p</em> &lt; 0.0001). Indices indicating <em>Blastocystis</em> specificity to host ranged between 0.16 (humans) and 0.49 (cattle) for the reference hosts, whereas indices for cats and dogs were only 0.01 and 0.02, respectively. Finally, indices for ST specificity to host were higher for reference hosts (range, 0.66–0.93) than for cats (0.62) and dogs (0.56). Taken together, the analyses indicate that cats and dogs are not natural or reservoir hosts of <em>Blastocystis</em> and that the sporadic subtype pattern observed in these hosts might indicate exposure to <em>Blastocystis</em> through contaminated water/feed, including <em>Blastocystis</em> colonizing prey animals.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110325"},"PeriodicalIF":2.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of the fungus Purpureocillium lilacinum applied via drone onto pasture for controlling the tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)","authors":"José Roberto Pereira ,&nbsp;Fernando Garcia Nicodemos ,&nbsp;Fernanda Calvo Duarte ,&nbsp;Jose Eduardo Marcondes de Almeida ,&nbsp;Márcia Cristina Mendes","doi":"10.1016/j.vetpar.2024.110323","DOIUrl":"10.1016/j.vetpar.2024.110323","url":null,"abstract":"<div><div>In the southeastern region of Brazil, ticks of the species <em>Rhipicephalus</em> (<em>B</em>.) <em>microplus</em> are constantly present on cattle throughout the year. This is due to climatic conditions that favor the biology of these ticks and resistance to the acaricides in use that has developed. The aim of this study was to evaluate the efficacy of the fungus <em>Purpureocillium lilacinum</em> (IBCB 130) in powdered form, applied via a drone, from the bioFUNGUS spray dispenser (NCB Sistemas Embarcados Ltda), onto pasture for controlling these ticks. This experiment was conducted at Aptaregional, Regional Research and Development Unit of Pindamonhangaba, São Paulo, Brazil. To evaluate the efficacy of treatment, two groups were formed (treated and control), each with ten calves of the Girolando breed, which were kept in two separate paddocks in which the main grass species was <em>Brachiaria decumbens</em>. The treated paddock received seven monthly applications (November–May) of the fungus <em>Purpureocillium lilacinum</em> (5 g de <em>P. lilacinum</em> in 200 g of wheat flour). The control paddock did not receive any treatment. Tick counts performed on the animals every two weeks showed that, over the entire period, the treatment had a mean efficacy of 48.59 %. The months of December and January presented the highest efficacy rates, of 63.50 % and 83.87 %, respectively.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"332 ","pages":"Article 110323"},"PeriodicalIF":2.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}