Biochimie最新文献_第8页

Phenolic acids inhibit local and systemic effects induced by Bothrops brazili venom 酚酸抑制巴西红腹虫毒液引起的局部和全身效应。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.08.003

Sarah de Sousa Ferreira , Diana Pontes da Silva , Manoela Torres-Rêgo , Allanny Alves Furtado , Eduardo Augusto da Silva Diniz , Karla Patrícia de Oliveira Luna , Arnóbio Antônio da Silva-Júnior , Renata Mendonça Araújo , Marcela Abbott Galvão Ururahy , Davi Serradella Vieira , Matheus de Freitas Fernandes-Pedrosa

Bothropic envenomation induces local damage, such as edema, necrosis, and hemorrhage, as well as systemic effects, including cardiovascular, renal and coagulation disturbances. While serum therapy remains, the primary treatment used for snakebites and efficiently neutralizes systemic damage, it inadequately addresses local tissue injury. Aiming to understand the pathophysiological mechanism of Bothrops brazili envenomation and explore novel complementary treatments, this study evaluated the local and systemic injuries caused by B. brazili venom and their mitigation by chlorogenic and rosmarinic acids. The venom exhibited high proteolytic and phospholipase activities, caused inflammation with edema formation, increased myeloperoxidase and cytokine dosage (IL-1β and IL-6). These effects were attenuated by phenolic acids. Molecular docking analysis assessed the interaction of these acids with B. brazili venom toxins, specifically catalytic PLA₂ (BbTX-III) and non-catalytic Lys49-PLA₂ (MTX-II). Chlorogenic and rosmarinic acids showed superior binding energies with MTX-II (−135.5683 ± 45.8415 kcal/mol and −166.8876 ± 17.7874 kcal/mol, respectively) compared to BbTX-III (−120.0387 ± 7.4546 kcal/mol and −114.3389 ± 15.4885 kcal/mol, respectively). Furthermore, these acids reduced myotoxicity, hemorrhage, hemostatic disturbances, and kidney and liver injuries, as well as leukogram and platelet alterations induced by B. brazili venom. The chlorogenic and rosmarinic acids demonstrated antiophidic potential by inhibiting both the local and systemic effects of envenomation. These findings suggest that their potential use as complementary therapies against envenomation caused by B. brazili.

体液中毒引起局部损害，如水肿、坏死和出血，以及全身影响，包括心血管、肾脏和凝血障碍。虽然血清疗法仍然是蛇咬伤的主要治疗方法，可以有效地中和全身损伤，但它不能充分解决局部组织损伤。本研究旨在了解巴西红牛毒液中毒的病理生理机制，探索新的辅助治疗方法，评估了巴西红牛毒液引起的局部和全身损伤，以及绿原酸和迷迭香酸对这些损伤的缓解作用。该毒液具有较高的蛋白水解酶和磷脂酶活性，引起炎症和水肿形成，增加髓过氧化物酶和细胞因子（IL-1β和IL-6）的剂量。这些作用被酚酸所减弱。分子对接分析评估了这些酸与巴西白螺旋体毒液毒素的相互作用，特别是催化PLA2 （BbTX-III）和非催化Lys49-PLA2 （MTX-II）。绿原酸和迷香酸与MTX-II的结合能（分别为-135.5683±45.8415 kcal/mol和-166.8876±17.7874 kcal/mol）优于BbTX-III的结合能（分别为-120.0387±7.4546 kcal/mol和-114.3389±15.4885 kcal/mol）。此外，这些酸还能减轻巴西弧菌毒液引起的肌毒性、出血、止血障碍、肾和肝损伤以及白细胞图和血小板改变。绿原酸和迷迭香酸通过抑制局部和全身的毒性作用显示出抗蛇毒作用的潜力。这些发现表明，它们有可能作为巴西芽孢杆菌引起的中毒的补充疗法。

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引用次数: 0

Codon usage and antibiotic resistance: A hidden evolutionary mechanism 密码子使用与抗生素耐药性：一个隐藏的进化机制。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.07.027

Ujwal Dahal, Anu Bansal

Antibiotic resistance represents a global health crisis, and emerging evidence suggests that codon usage, traditionally considered a silent aspect of genetic coding, plays a pivotal role in the evolution of resistance. Traditional resistance mechanisms, such as efflux pumps, enzymatic inactivation, and target modification, have been extensively studied. Still, recent findings highlight the role of codon optimization in enhancing the synthesis of resistance determinants in pathogens, including Acinetobacter baumannii, Neisseria gonorrhoeae, and Klebsiella pneumoniae. Comparative genomic analyses employing metrics such as Codon Adaptation Index (CAI), Effective Number of Codons (ENC), and Relative Synonymous Codon Usage (RSCU), alongside advanced bioinformatic and machine learning approaches, have identified subtle yet significant shifts in codon usage patterns between resistant and susceptible strains. Additionally, experimental studies using in vitro assays, in vivo models, and synthetic biology approaches prove that translational control through codon modulation contributes to adaptive responses under antibiotic pressure. Understanding these associations offers potential avenues for developing novel diagnostic biomarkers and therapeutic strategies. Therefore, this review underscores the necessity of an interdisciplinary approach to decipher the complex interplay between codon usage and antibiotic resistance, ultimately informing future efforts to mitigate the impact of multidrug-resistant pathogens.

抗生素耐药性是一场全球健康危机，新出现的证据表明，密码子的使用，传统上被认为是遗传编码的一个沉默方面，在耐药性的演变中起着关键作用。传统的耐药机制，如外排泵、酶失活和靶标修饰，已经得到了广泛的研究。尽管如此，最近的研究结果强调了密码子优化在增强病原体耐药决定因子合成中的作用，包括鲍曼不动杆菌、淋病奈瑟菌和肺炎克雷伯菌。采用诸如密码子适应指数（CAI）、有效密码子数量（ENC）和相对同义密码子使用（RSCU）等指标的比较基因组分析，以及先进的生物信息学和机器学习方法，已经确定了抗性和易感菌株之间密码子使用模式的微妙但显著的变化。此外，使用体外分析、体内模型和合成生物学方法的实验研究证明，通过密码子调节的翻译控制有助于抗生素压力下的适应性反应。了解这些关联为开发新的诊断生物标志物和治疗策略提供了潜在的途径。因此，这篇综述强调了跨学科方法的必要性，以破译密码子使用和抗生素耐药性之间的复杂相互作用，最终为未来减轻多重耐药病原体的影响提供信息。

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引用次数: 0

The unconventional RNA-binding activity of metabolic enzymes 代谢酶的非常规rna结合活性。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.08.017

Ana Luisa Dian , Stéphan Vagner

Metabolism involves a wide range of pathways and chemical reactions catalysed by specialized enzymes whose activity is fundamental for living cells. In the past three decades, metabolic enzymes have emerged as critical regulators of gene expression, thus revealing unexpected functions beyond their canonical metabolic roles. In this Review, we discuss the evidences that these enzymes, with a particular focus on enzymes participating in the glucose metabolism, can directly bind RNA. This binding has been recurrently shown to be involved in the post-trasncriptional gene regulation, by influencing processes such as RNA stability, localization, translation, and degradation. Although the mechanisms underlying RNA-enzyme interactions and their regulation are still not fully elucidated, several reports suggest that some of these interactions can be influenced by substrates, metabolites, and cellular metabolic states. In contrast, direct and specific binding of RNAs was also shown to regulate the activity, stability, interaction and localization of the enzymes. The discovery of the non-canonical RNA-binding activity of metabolic enzymes not only expands our understanding of these seemingly well-characterized proteins, but also provides new perspectives on the integration of metabolic and gene regulatory networks, besides revealing potential therapeutic vulnerabilities.

代谢包括广泛的途径和化学反应，由特定的酶催化，其活性是活细胞的基础。在过去的三十年中，代谢酶已经成为基因表达的关键调节因子，从而揭示了超出其规范代谢作用的意想不到的功能。在本文中，我们讨论了这些酶的证据，特别是参与糖代谢的酶，可以直接结合RNA。这种结合通过影响RNA稳定性、定位、翻译和降解等过程，反复被证明参与转录后基因调控。尽管rna -酶相互作用及其调控的机制尚未完全阐明，但一些报道表明，其中一些相互作用可能受到底物、代谢物和细胞代谢状态的影响。相反，rna的直接和特异性结合也被证明可以调节酶的活性、稳定性、相互作用和定位。代谢酶的非规范rna结合活性的发现不仅扩大了我们对这些看似特征明确的蛋白质的理解，而且除了揭示潜在的治疗脆弱性外，还为代谢和基因调控网络的整合提供了新的视角。

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引用次数: 0

Divergent clinical, inflammatory, and histopathological responses induced by Amazonian Tityus venoms: insights and limitations of current antivenom therapy 亚马逊Tityus毒液诱导的不同临床，炎症和组织病理学反应：当前抗蛇毒血清治疗的见解和局限性。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.08.004

Karina Furlani Zoccal , Karla de Castro Figueiredo Bordon , Mouzarllem Barros Reis , Paloma Beatriz Rosa Nunes de Souza Chini , Jonas Gama Martins , Beatriz Acquaroni Zuanazzi , Gisele Adriano Wiezel , Ana Carolina Campos dos Santos , Rudi Emerson de Lima Procópio , Eliane Candiani Arantes

Scorpion stings are considered a neglected condition and represent a serious health problem in many tropical countries, especially for children and the elderly. In Brazil, the yellow scorpion (Tityus serrulatus) is widely found and responsible for the majority of severe envenoming cases; however, other medically relevant species endemic to the Brazilian Amazon region, such as Tityus silvestris, Tityus metuendus and Tityus obscurus, remain underexplored. In the present study, we characterized the clinical, inflammatory and histopathological responses induced by venoms from these Amazonian species in a murine model (Balb/c mice), using T. serrulatus as a reference. Envenomation with T. silvestris resulted in pronounced systemic manifestations, including elevated clinical scores, hyperglycemia, leukocytosis, cytokine release (IL-6, IL-1β, IL-10), and tissue injury in the lungs and kidneys, comparable to the pathophysiological manifestations from T. serrulatus venom. In contrast, T. metuendus and T. obscurus induced milder inflammatory profiles. It is noteworthy that cross-reactivity assays revealed limited immunoreactivity and reduced in vivo neutralization of T. metuendus and T. obscurus venoms by the commercially available T. serrulatus-based antivenom. These findings reveal critical limitations in relying on a single-species antivenom for treating scorpion envenomation across diverse regions and underscore the need for region-specific therapeutic strategies tailored to the distinct venom profiles and pathogenicity of Amazonian Tityus species.

蝎子蜇伤被认为是一种被忽视的疾病，在许多热带国家是一个严重的健康问题，对儿童和老人来说尤其如此。在巴西，黄蝎子（Tityus serrulatus）广泛存在，大多数严重的中毒病例都是由黄蝎子引起的；然而，巴西亚马逊地区特有的其他与医学相关的物种，如Tityus silvestris、Tityus metuendus和Tityus obscurus，仍未得到充分探索。在本研究中，我们在小鼠模型（Balb/c小鼠）中描述了这些亚马逊物种的毒液诱导的临床、炎症和组织病理学反应，并以serrulatus为参考。西林螺旋体毒液中毒导致明显的全身表现，包括临床评分升高、高血糖、白细胞增多、细胞因子释放（IL-6、IL-1β、IL-10）以及肺和肾脏组织损伤，与蛇螺旋体毒液的病理生理表现相当。相比之下，大尾绦虫和隐尾绦虫引起的炎症较轻。值得注意的是，交叉反应性试验显示，市售的基于serrulatatus的抗蛇毒血清对大尾弓形虫和暗尾弓形虫的免疫反应性有限，体内中和性降低。这些发现揭示了依赖单一物种抗蛇毒血清治疗不同地区蝎子中毒的关键局限性，并强调了针对亚马逊Tityus物种独特的毒液特征和致病性量身定制区域特异性治疗策略的必要性。

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引用次数: 0

TSPO, reactive oxygen species and oxidative stress in physiological and pathological situations: a complex relationship TSPO、活性氧和氧化应激在生理和病理情况下的复杂关系。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.08.002

Didier Morin , Jean-Jacques Lacapère

Since its discovery in 1977 in the external membrane of mitochondria, the precise role of TSPO, previously named PBR for peripheral type benzodiazepine receptor, remains an enigma because of its interactions with various exogenous and endogenous ligands such as cholesterol, porphyrins and its implication in different cellular processes. Moreover, its presence in organisms ranging from bacteria to humans and its wide distribution in the body, including central nervous system, asked the question of its conserved function. Its involvement in many diseases often connected to inflammation led to the development of many positrons emission tomography tracers, but also questioned its link to the production of reactive oxygen species (ROS) well described in the inflammation processes. This critical review presents the various systems involving TSPO that produce ROS, such as for instance the respiratory chain or the NADPH oxidases. The protein partners that have been described to interact with TSPO in such processes are also presented. Finally, the relationships of TSPO with diseases implicating ROS are overviewed.

自1977年在线粒体外膜发现TSPO以来，由于其与各种外源性和内源性配体（如胆固醇、卟啉）的相互作用及其在不同细胞过程中的意义，TSPO的确切作用仍然是一个谜。此外，它存在于从细菌到人类的各种生物中，并广泛分布于人体，包括中枢神经系统，这就提出了它的保守功能的问题。它参与了许多与炎症相关的疾病，导致了许多正电子发射断层扫描示踪剂的发展，但也质疑它与炎症过程中活性氧（ROS）产生的联系。这篇重要的综述介绍了涉及TSPO产生ROS的各种系统，例如呼吸链或NADPH氧化酶。还介绍了在这些过程中与TSPO相互作用的蛋白质伙伴。最后，综述了TSPO与ROS相关疾病的关系。

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引用次数: 0

Inside front cover-EDB 内部前盖- edb

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/S0300-9084(25)00251-2

引用次数: 0

5.8S rRNA forms and ribosome heterogeneity in breast cancer 5.8S rRNA形式与乳腺癌核糖体异质性

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.05.010

Giulia Venturi , Federico Zacchini , Francesca Ruzzi , Angelo Gianluca Corradini , Margherita Serra , Marianna Penzo , Davide Treré , Pier-Luigi Lollini , Lorenzo Montanaro

Ribosome heterogeneity can contribute to translation regulation in terms of mRNA selection and translation efficiency. This is particularly true for cancer cells in which oncoribosomes are reported to translate mRNAs encoding for proteins involved in cancer progression. Among other factors, a source of ribosome heterogeneity not yet characterized could be represented by 5.8S rRNA and its three forms distinguished based on their 5’ sequence. So far, little is known about the role of the presence of these isoforms in mature ribosomes and how they may contribute to human pathology. Here we investigated the relative abundance of the three 5.8S rRNA isoforms in different contexts. Analyzing total and polysomal RNA from cancer cell lines we detected all the three forms recruited in actively translating ribosomes consistently to the basal levels of their expression. Moreover, we showed that changes in the relative abundance of 5.8S rRNA isoforms can be linked to the process of tumorigenesis in a human HER2 transgenic mouse model which develops spontaneous mammary carcinomas. Finally, from the analysis of breast cancer samples, we observed significant correlations between tumor grade, estrogen receptor status and patient prognosis with the relative abundance of 5.8S rRNA isoforms. These results suggest an additional level of complexity involving 5.8S rRNA and ribosome heterogeneity in cancer pathology.

核糖体异质性在mRNA选择和翻译效率方面有助于翻译调控。对于癌细胞来说尤其如此，据报道，在癌细胞中，核糖体可以翻译编码参与癌症进展的蛋白质的mrna。在其他因素中，一个尚未被表征的核糖体异质性来源可以用5.8S rRNA及其基于其5'序列区分的三种形式来表示。到目前为止，人们对这些同种异构体在成熟核糖体中的作用以及它们如何影响人类病理知之甚少。在这里，我们研究了三种5.8S rRNA亚型在不同环境下的相对丰度。分析来自癌细胞系的总RNA和多体RNA，我们检测到所有三种形式的核糖体在积极翻译核糖体时都被招募到其表达的基础水平。此外，我们发现5.8S rRNA亚型相对丰度的变化可能与发生自发性乳腺癌的人类HER2转基因小鼠模型的肿瘤发生过程有关。最后，通过对乳腺癌样本的分析，我们发现肿瘤分级、雌激素受体状态和患者预后与5.8S rRNA亚型的相对丰度存在显著相关性。这些结果表明，在癌症病理中涉及5.8S rRNA和核糖体异质性的额外复杂性。

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引用次数: 0

Genetically modified NK cells equipped with a switchable CAR for the treatment of HER2-positive cancers 配备可切换CAR的转基因NK细胞用于治疗her2阳性癌症。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.07.021

Maria A. Streltsova , Anna A. Boyko , Nadezhda A. Alekseeva , Galina M. Proshkina , Elena I. Shramova , Maria V. Grechikhina , Marina A. Shevchenko , Olga A. Shustova , Aleksey I. Popodko , Elena V. Konovalova , Aleksey A. Schulga , Alexander M. Sapozhnikov , Sergey M. Deyev , Elena I. Kovalenko

This study is focused on the development and characterization of NK-92 cells bearing a switchable chimeric antigen receptor (CAR) system for targeting HER2-positive breast cancers. The system employs a universal CAR framework based on the high-affinity interaction between barstar within the CAR (BsCAR) and barnase in the antigen-specific module. The barnase component was fused with an engineered ankyrin repeat protein (DARPin) specifically recognizing the HER2 tumor antigen. NK-92 cells were successfully modified to express BsCAR, while control mock-NK92 cells were generated with a variant of BsCAR incapable of surface expression.

Flow cytometry analysis confirmed successful transduction and proper surface expression of the BsCAR construct. The cytotoxic potential of the modified cells was evaluated through multiple approaches, including degranulation activity measurements, target cell lysis assays, and three-dimensional spheroid models. In the presence of the HER2-specific targeting module (Da-9.29-Bn), BsCAR-NK92 cells demonstrated significant and specific cytotoxicity against HER2-positive tumor cells, particularly those with high HER2 expression (SKBR3, SKOV-Kat, BT-474). The specificity of the system was confirmed using MCF7 cells expressing low levels of HER2 as controls.

In three-dimensional models, BsCAR-NK92 cells maintained their cytotoxic activity. These findings demonstrate the potential of BsCAR-NK92 cells as an “off-the-shelf” therapeutic approach for targeting HER2-positive cancers, offering a flexible platform that can be adapted to target different tumor antigens through the modular barnase-barstar system.

本研究的重点是开发和表征NK-92细胞携带可切换嵌合抗原受体（CAR）系统靶向her2阳性乳腺癌。该系统采用基于CAR内barstar （BsCAR）和抗原特异性模块中的barnase之间高亲和力相互作用的通用CAR框架。将这种藤蔓酶成分与特异性识别HER2肿瘤抗原的工程锚蛋白重复序列蛋白（DARPin）融合。NK-92细胞成功地表达了BsCAR，而对照模拟nk92细胞则产生了一种不能表面表达的BsCAR变体。流式细胞术分析证实了BsCAR结构的成功转导和正确的表面表达。通过多种方法评估修饰细胞的细胞毒性潜力，包括脱颗粒活性测量，靶细胞裂解测定和三维球体模型。在HER2特异性靶向模块（Da-9.29-Bn）存在下，BsCAR-NK92细胞对HER2阳性肿瘤细胞，特别是HER2高表达的肿瘤细胞（SKBR3, SKOV-Kat, BT-474）表现出显著的特异性细胞毒性。用表达低水平HER2的MCF7细胞作为对照，证实了该系统的特异性。在三维模型中，BsCAR-NK92细胞保持其细胞毒活性。这些发现证明了BsCAR-NK92细胞作为靶向her2阳性癌症的“现成”治疗方法的潜力，提供了一个灵活的平台，可以通过模块化barnase-barstar系统适应不同的肿瘤抗原。

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引用次数: 0

Bacillus subtilis 6S-1 RNA regulates transcription of genes related to surfactin biosynthesis 枯草芽孢杆菌6S-1 RNA调控表面素生物合成相关基因的转录。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.08.008

V.S. Trefilov , E.Y. Lindin , D.E. Elkina , M.I. Zvereva , V.A. Alferova , M.A.C. Schlüter , R.K. Hartmann , E.A. Kubareva , O.Y. Burenina

The biosurfactant surfactin is a cyclic lipopeptide produced by different representatives of the genus Bacillus. It has a large surface activity and is considered as a prospective biodegradable detergent. In the present study, we investigated the impact of non-coding 6S-1 RNA – a global transcription regulator in bacteria – on surfactin biosynthesis in the undomesticated B. subtilis strain NCIB 3610. It was found by RT-qPCR that a 6S-1 RNA knockout (KO) (ΔbsrA, ΔA) leads to increase of mRNA levels of several genes positively affecting surfactin biosynthesis, in particular the surfactin synthetase operon srfA. However, the lack of 6S-1 RNA did not result in an increase of surfactin amounts secreted by NCIB 3610 cells, as was shown by colorimetric and HPLC methods. Likewise, expression analysis of a GFP reporter construct provided no evidence that elevated transcript levels derived from the srfA promoter are paralleled by higher levels of translation products in the ΔA strain. We further identified a number of key factors also dysregulated in the ΔA strain, including a 20-fold increased level of rocG mRNA encoding glutamate dehydrogenase, an enzyme that may deplete the building block L-glutamate required for surfactin synthesis. Here we present the first study that evaluates the role of 6S-1 RNA in surfactin biosynthesis. We demonstrated that the absence of the riboregulator 6S-1 RNA profoundly destabilizes mRNA levels of genes, related to surfactin biosynthesis, in the undomesticated B. subtilis NCIB 3610.

生物表面活性剂表面素是由芽孢杆菌属的不同代表产生的环状脂肽。它具有很高的表面活性，被认为是一种有前景的生物可降解洗涤剂。在本研究中，我们研究了非编码6S-1 RNA（细菌中的全局转录调节因子）对未驯化的枯草芽孢杆菌NCIB 3610中表面素生物合成的影响。RT-qPCR发现，6S-1 RNA敲除（KO）（ΔbsrA， ΔA）导致几个正向影响表面素生物合成的基因mRNA水平升高，特别是表面素合成酶操纵子srfA。然而，通过比色法和高效液相色谱法显示，缺乏6S-1 RNA并没有导致NCIB 3610细胞分泌的表面素数量增加。同样，对GFP报告结构的表达分析也没有提供证据表明srfA启动子的转录产物水平升高与ΔA菌株中较高水平的翻译产物平行。我们进一步确定了一些关键因素也在ΔA菌株中失调，包括编码谷氨酸脱氢酶的rocG mRNA水平增加20倍，这种酶可能耗尽表面素合成所需的构建块l -谷氨酸。在这里，我们提出了第一项研究，评估了6S-1 RNA在表面素生物合成中的作用。我们证明，在未驯化的枯草芽孢杆菌NCIB 3610中，缺乏核素调节因子6S-1 RNA会严重破坏与表面素生物合成相关的基因mRNA水平。

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引用次数: 0

Hijacking a real time detection thermocycler for enzymology: Improvement of a fluorescent bulk assay monitoring helicase activity 劫持酶学实时检测热循环仪：荧光体检测解旋酶活性的改进。

IF 3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimie

Pub Date : 2025-11-01 DOI: 10.1016/j.biochi.2025.02.002

Jean-Philippe Robin, Vincent Mocquet

Helicases are enzymes involved in all aspects of nucleic acid synthesis, regulation and degradation. As a consequence, several methods were developed to monitor their enzymatic activity. In this report, we described an improvement of bulk fluorescent helicase assays to overcome their specific limitations (cost, health and safety regulations, etc.). Using a real time detection thermocycler to monitor the fluorescence in real-time, we managed to precisely control the initiation of the helicase reaction through temperature tuning. Therefore, we were able to demonstrate that this setup could provide a qualitative and a quantitative evaluation of the helicase domain of the UPF1 helicase (UPF1-HD) and that several fluorophores could be used in parallel during the same run.

解旋酶是参与核酸合成、调控和降解各个方面的酶。因此，开发了几种方法来监测它们的酶活性。在本报告中，我们描述了一种改进的散装荧光解旋酶测定法，以克服其特定的局限性（成本，健康和安全法规等）。利用实时检测热循环仪实时监测荧光，通过温度调节精确控制解旋酶反应的起始。因此，我们能够证明这种设置可以提供UPF1解旋酶（UPF1- hd）解旋酶结构域的定性和定量评估，并且可以在同一运行期间并行使用多个荧光团。

引用次数: 0