A. Bello, H. Abdulsalam, J. Dauda, A. Mbaya, A.U. Mani
Blood glucose, serum total protein, and liver transaminases were determined in Yankasa sheep experimentally infected with Trypanosoma evansi (T. evansi) and treated with diminazene aceturate (Berenil® ). A total of 30 animals were divided into 6 groups (A to F) (n=5). Animals from each group were either uninfected or infected with T. evansi and treated with Berenil® . Infection of the infected groups (A, C and E) was done via intravenous inoculation of T. evansi, while the infected group C and E were treated with Berenil® at 3.5 and 7 mg/kg BW (single dose), respectively, by day 16 post-infection (PI). The infected groups had a pre-patent period of 8 days, with similar levels of parasitaemia of 4.7 ± 0.27. In group A, the mean parasite count rose significantly (p < 0.05) to 72.8 ± 1.07 by day 12 PI and continued to a peak value of 250.6 ± 1.98 by day 28 PI. In groups C and E, the initial parasitaemia rose significantly (p < 0.05) to a peak count of 80.8 ± 1.12 and 78.2 ± 1.11 by day 12 PI, following treatment with 3.5 and 7.0 mg/kg BW of Berenil® , by day 20 PI, respectively, and was completely eliminated by day 9 and 5 post-treatment (PT), respectively. The biochemical analysis showed that, from day 8 PI, the infected sheep experienced significant (p < 0.05) increases in alkaline phosphatase, aspartate and alanine aminotransferase, creatinine and urea; with decreases in blood glucose and total protein. However, these changes reverted to their pre-infection values, by day 28 PI in all the affected animals following treatment with both doses of Berenil® . It is therefore, concluded that the two doses of Berenil® (3.5 mg/kg and 7.0 mg/kg) were effective in the treatment of the disease but 7.0 mg/kg cleared the parasitaemia faster.
用乙酸二咪唑嗪（Berenil®）治疗实验性感染了埃文斯锥虫（T. evansi）的扬卡萨绵羊，测定其血糖、血清总蛋白和肝脏转氨酶。总共 30 只绵羊被分为 6 组（A 至 F 组）（n=5）。每组动物要么未感染 T. evansi，要么感染 T. evansi 并接受 Berenil® 治疗。感染组（A、C 和 E）通过静脉注射 T. evansi 进行感染，而感染组 C 和 E 则在感染后第 16 天（PI）前分别使用 3.5 和 7 mg/kg BW（单剂量）的 Berenil® 进行治疗。感染组的专利期前为 8 天，寄生虫血症水平相似，均为 4.7 ± 0.27。在 A 组，寄生虫平均数量在感染后第 12 天显著上升（P < 0.05）至 72.8 ± 1.07，并在感染后第 28 天达到 250.6 ± 1.98 的峰值。在 C 组和 E 组中，在使用 3.5 和 7.0 mg/kg BW 的 Berenil® 治疗后，初始寄生虫血症显著上升（p < 0.05），到第 12 天（PI）分别达到 80.8 ± 1.12 和 78.2 ± 1.11 的峰值，到第 20 天（PI）分别完全消除。生化分析表明，从治疗前第 8 天开始，感染羊的碱性磷酸酶、天门冬氨酸和丙氨酸氨基转移酶、肌酐和尿素显著增加（p < 0.05），血糖和总蛋白下降。 不过，在使用两种剂量的 Berenil® 治疗后，所有受影响动物的这些变化在第 28 天 PI 时均恢复到感染前的数值。因此，结论是两种剂量的 Berenil®（3.5 毫克/千克和 7.0 毫克/千克）都能有效治疗该病，但 7.0 毫克/千克能更快地清除寄生虫血症。
{"title":"Serum biochemical parameters of Yankasa sheep experimentally infected with Trypanosoma evansi and treated with diminazene aceturate (Berenil®)","authors":"A. Bello, H. Abdulsalam, J. Dauda, A. Mbaya, A.U. Mani","doi":"10.4314/sokjvs.v21i3.5","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.5","url":null,"abstract":"Blood glucose, serum total protein, and liver transaminases were determined in Yankasa sheep experimentally infected with Trypanosoma evansi (T. evansi) and treated with diminazene aceturate (Berenil® ). A total of 30 animals were divided into 6 groups (A to F) (n=5). Animals from each group were either uninfected or infected with T. evansi and treated with Berenil® . Infection of the infected groups (A, C and E) was done via intravenous inoculation of T. evansi, while the infected group C and E were treated with Berenil® at 3.5 and 7 mg/kg BW (single dose), respectively, by day 16 post-infection (PI). The infected groups had a pre-patent period of 8 days, with similar levels of parasitaemia of 4.7 ± 0.27. In group A, the mean parasite count rose significantly (p < 0.05) to 72.8 ± 1.07 by day 12 PI and continued to a peak value of 250.6 ± 1.98 by day 28 PI. In groups C and E, the initial parasitaemia rose significantly (p < 0.05) to a peak count of 80.8 ± 1.12 and 78.2 ± 1.11 by day 12 PI, following treatment with 3.5 and 7.0 mg/kg BW of Berenil® , by day 20 PI, respectively, and was completely eliminated by day 9 and 5 post-treatment (PT), respectively. The biochemical analysis showed that, from day 8 PI, the infected sheep experienced significant (p < 0.05) increases in alkaline phosphatase, aspartate and alanine aminotransferase, creatinine and urea; with decreases in blood glucose and total protein. However, these changes reverted to their pre-infection values, by day 28 PI in all the affected animals following treatment with both doses of Berenil® . It is therefore, concluded that the two doses of Berenil® (3.5 mg/kg and 7.0 mg/kg) were effective in the treatment of the disease but 7.0 mg/kg cleared the parasitaemia faster.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Bakam, B. Inuwa, W. Yiltawe, I. Shittu, C. Meseko, G. Kia
Since the first 2006 Avian Influenza (AI) outbreak in Nigeria, it has continued to circulate and ravage the poultry industry with huge economic losses above 130 billion naira. Local poultry are important sources of AI transmission and maintenance of the highly pathogenic avian influenza H5N1. Live bird markets (LBMs) are “breeding grounds” and major risk factors for human infection of AI. The seroprevalence of AI in local chickens in LBMs in Kaduna Metropolis, Nigeria was determined. Sera were obtained from the local chickens (n=300) in 5 daily LBMs and subjected to competitive enzyme-linked immunosorbent assay (c-ELISA) to detect the nucleoprotein antibodies. The c-ELISA positive samples were further screened by haemagglutination inhibition (HI) test for AI using H5, H7 and H9 antigens. The overall AI seroprevalence was 1.7 % with the highest individual seroprevalence of 3.3% in Kawo and Railway LBMs. There was no statistically significant association between the location of LBMs and AI presence, and between the sex and presence of AI antibodies (p = 0.427). One sample was HI positive for antibodies against H5, H7 and H9 with mean titers of 4, 2.5 and 3.5 log2, respectively. This implies the potential danger of the spread of AI among humans and animals. There should be sustained surveillance and biosecurity in the live bird market.
{"title":"Serosurvey for H5, H7 and H9 avian influenza viruses in local chickens in live bird markets within Kaduna metropolis, Nigeria","authors":"J. Bakam, B. Inuwa, W. Yiltawe, I. Shittu, C. Meseko, G. Kia","doi":"10.4314/sokjvs.v21i3.3","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.3","url":null,"abstract":"Since the first 2006 Avian Influenza (AI) outbreak in Nigeria, it has continued to circulate and ravage the poultry industry with huge economic losses above 130 billion naira. Local poultry are important sources of AI transmission and maintenance of the highly pathogenic avian influenza H5N1. Live bird markets (LBMs) are “breeding grounds” and major risk factors for human infection of AI. The seroprevalence of AI in local chickens in LBMs in Kaduna Metropolis, Nigeria was determined. Sera were obtained from the local chickens (n=300) in 5 daily LBMs and subjected to competitive enzyme-linked immunosorbent assay (c-ELISA) to detect the nucleoprotein antibodies. The c-ELISA positive samples were further screened by haemagglutination inhibition (HI) test for AI using H5, H7 and H9 antigens. The overall AI seroprevalence was 1.7 % with the highest individual seroprevalence of 3.3% in Kawo and Railway LBMs. There was no statistically significant association between the location of LBMs and AI presence, and between the sex and presence of AI antibodies (p = 0.427). One sample was HI positive for antibodies against H5, H7 and H9 with mean titers of 4, 2.5 and 3.5 log2, respectively. This implies the potential danger of the spread of AI among humans and animals. There should be sustained surveillance and biosecurity in the live bird market.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"61 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Kabantiyok, L.E. Logyan, A. Ogundeji, J. Budaye, I. Adonyikwu, G.O.A. Agada, G.D. Moses, A.A. Masooq
There is a growing concern for exploring the animal-human interface through surveillance of peri-urban animals. Wildlife at this interface has been reported to pick up and redistribute antimicrobial-resistant bacteria and other infectious diseases. Samples from two stray Owl fledglings found at the National Veterinary Research Institute, Nigeria were used for this study. Corynebacterium amycolatum, Mammalicoccus siuri, and Escherichia coli were isolated and identified from cloacal and oropharyngeal swabs collected from the birds. Almost all (4/5) of the isolates had multidrug resistance. Also, three species of Leptospira (L. enterohaemorrhagica. L grippotyphosa, and L. mini) were identified by the microscopic agglutination technique (MAT). Our findings emphasised the growing One Health concerns for antimicrobial resistance (AMR) spread in the environment and the importance of Wildlife in periurban centres as sentinels for potential zoonotic transmission.
人们越来越关注通过监测城市周边的动物来探索动物与人类的交界处。 据报道，在这个界面上的野生动物会拾取并重新传播抗微生物细菌和其他传染病。本研究采用了在尼日利亚国家兽医研究所发现的两只流浪猫头鹰雏鸟的样本。从这些鸟类的泄殖腔和口咽拭子中分离并鉴定出了淀粉样棒状杆菌（Corynebacterium amycolatum）、哺乳球菌（Mammalicoccus siuri）和大肠杆菌（Escherichia coli）。几乎所有的分离物（4/5）都具有多重耐药性。此外，还通过显微凝集技术（MAT）鉴定出三种钩端螺旋体（L. enterohaemorrhagica、L. grippotyphosa 和 L. mini）。我们的研究结果强调了人们对抗菌药耐药性（AMR）在环境中传播的日益关注，以及城郊中心野生动物作为潜在人畜共患病传播哨兵的重要性。
{"title":"Peri-urban wildlife as sentinels for antimicrobial resistance: Insights from owlets (Tyoto alba) in Jos, Nigeria","authors":"D. Kabantiyok, L.E. Logyan, A. Ogundeji, J. Budaye, I. Adonyikwu, G.O.A. Agada, G.D. Moses, A.A. Masooq","doi":"10.4314/sokjvs.v21i3.7","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.7","url":null,"abstract":"There is a growing concern for exploring the animal-human interface through surveillance of peri-urban animals. Wildlife at this interface has been reported to pick up and redistribute antimicrobial-resistant bacteria and other infectious diseases. Samples from two stray Owl fledglings found at the National Veterinary Research Institute, Nigeria were used for this study. Corynebacterium amycolatum, Mammalicoccus siuri, and Escherichia coli were isolated and identified from cloacal and oropharyngeal swabs collected from the birds. Almost all (4/5) of the isolates had multidrug resistance. Also, three species of Leptospira (L. enterohaemorrhagica. L grippotyphosa, and L. mini) were identified by the microscopic agglutination technique (MAT). Our findings emphasised the growing One Health concerns for antimicrobial resistance (AMR) spread in the environment and the importance of Wildlife in periurban centres as sentinels for potential zoonotic transmission.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139277373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dermatophytosis is a contagious skin disease affecting domestic and wild animals with considerable zoonotic significance. The disease is caused by fungi known as dermatophytes in the genera Trichophyton, Microsporum and Epidermophyton. A goat was observed with circumscribed alopecic, highly inflammatory, thickly crusted skin lesions on the head region, especially around the eyes. Skin scraping, including hair pullouts, was aseptically obtained and processed for direct examination, isolation and microscopic identification of etiologic agent and supplemented with urease test. The isolate was tested for its susceptibility to fluconazole, griseofulvin, itraconazol and ketoconazole. Direct examination revealed hyaline, septate hyphae in skin scales and chains of endothrix spores within hair shafts, suggesting Trichophyton infection. Colonies on Sabouraud’s dextrose agar were white, flat and granular. Microscopic examination of the isolate revealed many oval micro-conidia arranged in grapelike clusters with multi-septate, club- shaped, thin, and smooth-walled macroconidia typical of Trichophyton mentagrophytes. The isolate was urease-positive and sensitive to itraconazole and ketoconazole but resistant to fluconazole and griseofulvin. It was concluded that T. mentagrophytes was the cause of the severe skin lesions in the goat. The need to perform antifungal susceptibility testing on dermatophytes isolated from clinical specimens for effective management of dermatophytosis was emphasized.
{"title":"Characterization and antifungal susceptibility of Trichophyton mentagrophytes isolated from a goat presented with severe dermatophytosis in Zaria, Nigeria","authors":"J. Dalis, H. Kazeem, K.F. Chah","doi":"10.4314/sokjvs.v21i3.8","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.8","url":null,"abstract":"Dermatophytosis is a contagious skin disease affecting domestic and wild animals with considerable zoonotic significance. The disease is caused by fungi known as dermatophytes in the genera Trichophyton, Microsporum and Epidermophyton. A goat was observed with circumscribed alopecic, highly inflammatory, thickly crusted skin lesions on the head region, especially around the eyes. Skin scraping, including hair pullouts, was aseptically obtained and processed for direct examination, isolation and microscopic identification of etiologic agent and supplemented with urease test. The isolate was tested for its susceptibility to fluconazole, griseofulvin, itraconazol and ketoconazole. Direct examination revealed hyaline, septate hyphae in skin scales and chains of endothrix spores within hair shafts, suggesting Trichophyton infection. Colonies on Sabouraud’s dextrose agar were white, flat and granular. Microscopic examination of the isolate revealed many oval micro-conidia arranged in grapelike clusters with multi-septate, club- shaped, thin, and smooth-walled macroconidia typical of Trichophyton mentagrophytes. The isolate was urease-positive and sensitive to itraconazole and ketoconazole but resistant to fluconazole and griseofulvin. It was concluded that T. mentagrophytes was the cause of the severe skin lesions in the goat. The need to perform antifungal susceptibility testing on dermatophytes isolated from clinical specimens for effective management of dermatophytosis was emphasized.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"39 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regional anatomy is an important component of surgical and clinical practice because it helps clinicians envisage the specifics of the structures relevant to the case at hand. This study was designed to evaluate some clinically important indices which could aid regional anesthesia of the head in the Nigerian indigenous pig (NIP). Twenty-four (10 males and 14 females) skulls of adult NIP were examined to record 10 parameters. Data obtained were statistically analysed using the Student’s t-test and Pearson Correlation, with significance set at p<0.05. The study revealed that 50% of the indices were higher in female skulls but were not statistically significant. The mandibular length was, however, significantly longer in male skulls (19.0 ± 0.41), compared to females (18.0 ± 0.26). The distance from the facial crest to the infraorbital foramen was 1.3 ± 0.10 cm and 1.5 ± 0.08 cm, while that from the supraorbital foramen to the nasal bone was 2.1 ± 0.06 cm and 1.9 ± 0.06 cm, in male and female skulls, respectively. Pearson correlation analysis revealed a positive relationship between the distance from the facial crest to infraorbital foramen and that from infraorbital foramen to medial canthus of the orbit (r=.669). An accessory supraorbital foramen was seen in 12 (50%) of all the 24 skulls, just as several lateral mental foramina were observed on the rostro-lateral surface of the mandibular rami. Data generated provide clinically important information on the skull of adult Nigerian indigenous pigs which can be used as landmarks for tracking and desensitizing the branches of the frontal nerve (supraorbital), the maxillary nerve (Infraorbital) and the mandibular nerve, during the regional anaesthesia of the head, for tooth extraction procedures and the treatment of injuries and fractures. These results may also be useful for comparative anatomical, developmental, forensic, and clinical studies.
{"title":"Some aspects of the craniometry of adult Nigerian indigenous pigs (Sus scrofa) and its application in regional anaesthesia","authors":"M. Okandeji, Z.B. Olaniyi","doi":"10.4314/sokjvs.v21i3.1","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.1","url":null,"abstract":"Regional anatomy is an important component of surgical and clinical practice because it helps clinicians envisage the specifics of the structures relevant to the case at hand. This study was designed to evaluate some clinically important indices which could aid regional anesthesia of the head in the Nigerian indigenous pig (NIP). Twenty-four (10 males and 14 females) skulls of adult NIP were examined to record 10 parameters. Data obtained were statistically analysed using the Student’s t-test and Pearson Correlation, with significance set at p<0.05. The study revealed that 50% of the indices were higher in female skulls but were not statistically significant. The mandibular length was, however, significantly longer in male skulls (19.0 ± 0.41), compared to females (18.0 ± 0.26). The distance from the facial crest to the infraorbital foramen was 1.3 ± 0.10 cm and 1.5 ± 0.08 cm, while that from the supraorbital foramen to the nasal bone was 2.1 ± 0.06 cm and 1.9 ± 0.06 cm, in male and female skulls, respectively. Pearson correlation analysis revealed a positive relationship between the distance from the facial crest to infraorbital foramen and that from infraorbital foramen to medial canthus of the orbit (r=.669). An accessory supraorbital foramen was seen in 12 (50%) of all the 24 skulls, just as several lateral mental foramina were observed on the rostro-lateral surface of the mandibular rami. Data generated provide clinically important information on the skull of adult Nigerian indigenous pigs which can be used as landmarks for tracking and desensitizing the branches of the frontal nerve (supraorbital), the maxillary nerve (Infraorbital) and the mandibular nerve, during the regional anaesthesia of the head, for tooth extraction procedures and the treatment of injuries and fractures. These results may also be useful for comparative anatomical, developmental, forensic, and clinical studies.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"21 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139277585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. M. Jajere, D. Jidda, A.A. Idriss, M. A. Sadiq, H. I. Musa, A. Tijjani, S. Adamu, B.T. Paul, D. Dika1
Fish is an important and cheap protein source for human consumption in developing countries. It is the second most popular source of protein diet in both rural and urban communities in Nigeria. Fisheries resources contribute about 5.40% to the nation's gross domestic product (GDP) through income generation, employment, food security, and foreign exchange earnings. Despite the significant contributions of fisheries to the Nigerian economy, the aquatic ecosystem is prone to pollution from human activities which increases the chances of transmission of parasitic infections, which are detrimental to aquatic and human life. The present study assessed the prevalence of gastrointestinal helminths of tilapia fish from different market sources in the Maiduguri metropolitan council of Borno State, Northeastern Nigeria. Samples of fresh Nile tilapia (Oreochromis niloticus) fish (n= 125), comprising 50 juveniles and 75 adults, were screened for gastrointestinal helminths using standard parasitological methods. Twenty-nine (23.2%) harboured at least one helminth parasite. Of the 29 positive individual fish samples, 16% (n=20), 4% (n=5), 2.4% (n=3), and 0.8% (n=1) were infected by Acanthocephala, Cestode, Nematode, and Trematodes, respectively. The infection rate was higher in adults (26%) than juveniles (18%) and in females (23.5%) than males (22.8%). Fish samples from Baga markets showed higher infection rates than the other two market sources. Sex, age, and location were not significantly associated with parasite recovery rate (p>0.05). The study concluded that Nile tilapia fish sold in Maiduguri metropolis were infected with different groups of helminths, underscoring public health risks to farmers, sellers, and consumers. Therefore, fish farmers should regularly consult veterinarians for advice on good fish management practices and observe proper hygienic handling of harvested fish at the farm level, during transportation to fish markets, and processing. The public should thoroughly clean and properly cook fish for human consumption.
{"title":"Gastrointestinal helminths in tilapia (Oreochromis niloticus) sold for human consumption in Maiduguri, Nigeria: A potential public health risk","authors":"S. M. Jajere, D. Jidda, A.A. Idriss, M. A. Sadiq, H. I. Musa, A. Tijjani, S. Adamu, B.T. Paul, D. Dika1","doi":"10.4314/sokjvs.v21i3.6","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.6","url":null,"abstract":"Fish is an important and cheap protein source for human consumption in developing countries. It is the second most popular source of protein diet in both rural and urban communities in Nigeria. Fisheries resources contribute about 5.40% to the nation's gross domestic product (GDP) through income generation, employment, food security, and foreign exchange earnings. Despite the significant contributions of fisheries to the Nigerian economy, the aquatic ecosystem is prone to pollution from human activities which increases the chances of transmission of parasitic infections, which are detrimental to aquatic and human life. The present study assessed the prevalence of gastrointestinal helminths of tilapia fish from different market sources in the Maiduguri metropolitan council of Borno State, Northeastern Nigeria. Samples of fresh Nile tilapia (Oreochromis niloticus) fish (n= 125), comprising 50 juveniles and 75 adults, were screened for gastrointestinal helminths using standard parasitological methods. Twenty-nine (23.2%) harboured at least one helminth parasite. Of the 29 positive individual fish samples, 16% (n=20), 4% (n=5), 2.4% (n=3), and 0.8% (n=1) were infected by Acanthocephala, Cestode, Nematode, and Trematodes, respectively. The infection rate was higher in adults (26%) than juveniles (18%) and in females (23.5%) than males (22.8%). Fish samples from Baga markets showed higher infection rates than the other two market sources. Sex, age, and location were not significantly associated with parasite recovery rate (p>0.05). The study concluded that Nile tilapia fish sold in Maiduguri metropolis were infected with different groups of helminths, underscoring public health risks to farmers, sellers, and consumers. Therefore, fish farmers should regularly consult veterinarians for advice on good fish management practices and observe proper hygienic handling of harvested fish at the farm level, during transportation to fish markets, and processing. The public should thoroughly clean and properly cook fish for human consumption.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"61 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study evaluated the genetic profile of a low passaged BGM-70 adapted very virulent infectious bursal disease virus (vvIBDV) isolated in Malaysia in the year 2004 based on the VP2 hypervariable region. Embryonated chicken eggs were first used to propagate UPM190 12 times before being low passaged in BGM-70 cell line 7 times yielding UMP190BGMP7 whose VP2 nucleotide and amino acid sequences were determined and compared with reference sequences after Sanger sequencing of the low passage virus. The nucleotide and amino acid sequences of UPM190BGMP7 virus were similar with the sequences of viruses passaged in chicken embryonated eggs (CEE) but differed with higher BGM-70 passaged viruses. The E270 amino acid mutation associated with higher BGM-70 passaged viruses was absent in UPM190BGMP7. However, it has another amino acid mutation at position D279N that back-mutated on continuous BGM-70 cell propagation.
{"title":"Molecular characterization of the VP2 protein of BGM-70 adapted low passaged UPM190 infectious bursal disease virus isolate","authors":"U.N. Lawal, M.B. Bello","doi":"10.4314/sokjvs.v21i3.2","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.2","url":null,"abstract":"The study evaluated the genetic profile of a low passaged BGM-70 adapted very virulent infectious bursal disease virus (vvIBDV) isolated in Malaysia in the year 2004 based on the VP2 hypervariable region. Embryonated chicken eggs were first used to propagate UPM190 12 times before being low passaged in BGM-70 cell line 7 times yielding UMP190BGMP7 whose VP2 nucleotide and amino acid sequences were determined and compared with reference sequences after Sanger sequencing of the low passage virus. The nucleotide and amino acid sequences of UPM190BGMP7 virus were similar with the sequences of viruses passaged in chicken embryonated eggs (CEE) but differed with higher BGM-70 passaged viruses. The E270 amino acid mutation associated with higher BGM-70 passaged viruses was absent in UPM190BGMP7. However, it has another amino acid mutation at position D279N that back-mutated on continuous BGM-70 cell propagation.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"12 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the impact of age-related peripheral and Gonadotropin Releasing Hormone (GnRH) -stimulated hormonal profiles on testicular size in West African dwarf bucks. Twelve 3-month-old bucks were randomly divided into two groups: the GnRH- treated group (n = 6) and the control group (n = 6). Over the course of 10 months, from month 3 to month 12, the testicular diameter of all bucks was measured monthly, and blood samples were collected monthly for hormone analysis. The results showed that testicular diameter increased with age and higher concentrations of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone (T). The GnRHtreated group reached its peak testicular diameter (28.36±1.44 mm) earlier at 8 months compared to the control group (30.50±1.44 mm) at 10 months, aligning with the peak concentrations of FSH, LH, and T. While the GnRH stimulation led to higher FSH, LH, T, and estrogen levels in the treated group, it didn't significantly affect the final testicular diameter (p<0.05). It can be concluded that hormonal levels rise with age and increasing testicular size, and GnRH stimulation can accelerate this process. Testicular size is an important factor in breeding soundness and is influenced by LH, FSH, and testosterone concentrations. These hormones can reliably indicate the reproductive capacity of the buck. Therefore, hormonal profiling should be an integral part of the breeding soundness examination.
{"title":"Age-related changes in hormonal profiles and testicular diameter of West African Dwarf buck treated with gonadotropin-releasing hormone","authors":"I.S. Oruene, H.M. Mutembei, A.N. Kipyegon","doi":"10.4314/sokjvs.v21i3.4","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i3.4","url":null,"abstract":"This study aimed to investigate the impact of age-related peripheral and Gonadotropin Releasing Hormone (GnRH) -stimulated hormonal profiles on testicular size in West African dwarf bucks. Twelve 3-month-old bucks were randomly divided into two groups: the GnRH- treated group (n = 6) and the control group (n = 6). Over the course of 10 months, from month 3 to month 12, the testicular diameter of all bucks was measured monthly, and blood samples were collected monthly for hormone analysis. The results showed that testicular diameter increased with age and higher concentrations of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone (T). The GnRHtreated group reached its peak testicular diameter (28.36±1.44 mm) earlier at 8 months compared to the control group (30.50±1.44 mm) at 10 months, aligning with the peak concentrations of FSH, LH, and T. While the GnRH stimulation led to higher FSH, LH, T, and estrogen levels in the treated group, it didn't significantly affect the final testicular diameter (p<0.05). It can be concluded that hormonal levels rise with age and increasing testicular size, and GnRH stimulation can accelerate this process. Testicular size is an important factor in breeding soundness and is influenced by LH, FSH, and testosterone concentrations. These hormones can reliably indicate the reproductive capacity of the buck. Therefore, hormonal profiling should be an integral part of the breeding soundness examination.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"28 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SC Attama, SC David, PF Egunleti, N. Okwelum, TU Obetta
This study aimed to evaluate the antioxidant effect and the state of the liver following injury by alloxan monohydrate and treatment with an aqueous extract of Senna alata (SA) leaf. A total of 24 male Albino Wistar rats assigned into 4 groups (A-D) of 6 rats per group were used in this study. Forty-eight hours following administration of alloxan monohydrate and confirmation of diabetes mellitus in groups B-D rats, group C were treated with SA while group D rats were treated with glibenclamide. Rats in groups A and B received distilled water. The treatments were through oral administration, once daily for 21 consecutive days. On day 21 post-treatment, serum samples were collected for in vivo antioxidants and liver enzyme assays. The liver tissues were also collected for macroscopic examination and histomorphology. The in vitro antioxidant activity of SA was also determined using DPPH photometric model. The SA-treated rats recorded significantly reduced activities of ALT, ALP and total bilirubin values when compared to rats in the diabetic untreated group. There was also a significant increase in the activities of catalase, superoxide dismutase and glutathione in SA-treated rats compared to those of the untreated diabetic group. The study also recorded 67.33% in vitro antioxidant activity of SA at 400 μg/ml. Liver photomicrographs of rats treated with the extract were comparable to those of the normal control while diabetic untreated rats showed congestion of the central vein, degeneration and necrosis of hepatocytes. SA has both in vitro and in vivo antioxidant activity and protected the liver against damage by alloxan monohydrate.
{"title":"Antioxidant and antidiabetic effects of aqueous extract of Senna alata on alloxan-induced diabetic rats","authors":"SC Attama, SC David, PF Egunleti, N. Okwelum, TU Obetta","doi":"10.4314/sokjvs.v21i2.3","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i2.3","url":null,"abstract":"This study aimed to evaluate the antioxidant effect and the state of the liver following injury by alloxan monohydrate and treatment with an aqueous extract of Senna alata (SA) leaf. A total of 24 male Albino Wistar rats assigned into 4 groups (A-D) of 6 rats per group were used in this study. Forty-eight hours following administration of alloxan monohydrate and confirmation of diabetes mellitus in groups B-D rats, group C were treated with SA while group D rats were treated with glibenclamide. Rats in groups A and B received distilled water. The treatments were through oral administration, once daily for 21 consecutive days. On day 21 post-treatment, serum samples were collected for in vivo antioxidants and liver enzyme assays. The liver tissues were also collected for macroscopic examination and histomorphology. The in vitro antioxidant activity of SA was also determined using DPPH photometric model. The SA-treated rats recorded significantly reduced activities of ALT, ALP and total bilirubin values when compared to rats in the diabetic untreated group. There was also a significant increase in the activities of catalase, superoxide dismutase and glutathione in SA-treated rats compared to those of the untreated diabetic group. The study also recorded 67.33% in vitro antioxidant activity of SA at 400 μg/ml. Liver photomicrographs of rats treated with the extract were comparable to those of the normal control while diabetic untreated rats showed congestion of the central vein, degeneration and necrosis of hepatocytes. SA has both in vitro and in vivo antioxidant activity and protected the liver against damage by alloxan monohydrate.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"22 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127886893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AA Biu, M. Abdulkadir, TE Onyiche, ZA Muhammad, J. Musa
The development of anthelmintic resistance and the high cost of conventional anthelmintics have led to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in vitro experiments were conducted to determine the possible anthelmintic effects of Balanites aegyptiaca stem bark aqueous extract on Strongyle larvae and the earthworm Pheretima posthuma. The in vitro studies revealed that the extract at graded concentrations of 300 mg/ml, 400 mg/ml, 500 mg/ml and 600 mg/ml exhibited larvicidal activity (p<0.05) against earthworms with 600 mg/ml having the highest activity causing paralysis and death. In a similar vein, a graded concentration response was observed against Strongyle larvae. The highest effective concentration on larvae was 250 mg/ml which had a larval mortality rate of 100% and a mortality index of one (1) comparable with that of albendazole. The IC50 and IC99 values for the time of paralysis were 442.7mg/ml and 767.80mg/ml respectively. The anthelmintic activity exhibited by the extract could be linked to the presence of phytochemicals present in the plant. These findings support the folkloric use of this plant in the control and management of gastrointestinal nematodes in humans and animals.
{"title":"Anthelmintic effects of aqueous extract Balanites aegyptiaca stem bark on strongyle larvae and the earthworm Pheretima posthuma","authors":"AA Biu, M. Abdulkadir, TE Onyiche, ZA Muhammad, J. Musa","doi":"10.4314/sokjvs.v21i2.1","DOIUrl":"https://doi.org/10.4314/sokjvs.v21i2.1","url":null,"abstract":"The development of anthelmintic resistance and the high cost of conventional anthelmintics have led to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in vitro experiments were conducted to determine the possible anthelmintic effects of Balanites aegyptiaca stem bark aqueous extract on Strongyle larvae and the earthworm Pheretima posthuma. The in vitro studies revealed that the extract at graded concentrations of 300 mg/ml, 400 mg/ml, 500 mg/ml and 600 mg/ml exhibited larvicidal activity (p<0.05) against earthworms with 600 mg/ml having the highest activity causing paralysis and death. In a similar vein, a graded concentration response was observed against Strongyle larvae. The highest effective concentration on larvae was 250 mg/ml which had a larval mortality rate of 100% and a mortality index of one (1) comparable with that of albendazole. The IC50 and IC99 values for the time of paralysis were 442.7mg/ml and 767.80mg/ml respectively. The anthelmintic activity exhibited by the extract could be linked to the presence of phytochemicals present in the plant. These findings support the folkloric use of this plant in the control and management of gastrointestinal nematodes in humans and animals.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126755172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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