DO Omoniwa, C. Chinyere, ER Agusi, N. Mkpuma, J. Oyetunde, OE Igah, Adole, A.M. Adidu-Omoniwa, C. Meseko
Canine influenza is a highly contagious respiratory infection of dogs caused by the Influenza A Virus (IAV), characterized by cough, sneeze, nasal secretions, and inappetence. Infections can be mild, severe or fatal. Aquatic birds constitute a natural reservoir for IAV, which is transmitted to terrestrial birds, including poultry. IAV has also emerged in other mammalian species, including humans, swine, horses, and dogs. IAV epidemics in dogs are a recent development. Commonly detected Canine Influenza Virus (CIV) strains are A/H3N2 and A/H3N8 from avian and equine influenza, respectively. Nigeria’s agro-ecology witnessed widespread circulation of avian influenza since 2006, and recent outbreaks of equine influenza in 2018/2019 raise the possibility of inter-species transmission to dogs. To investigate canine Influenza in Plateau State, we collected 113 nasal swabs and 270 sera samples from dogs in clinics, live dog markets, and during dog vaccination campaigns. After extracting nucleic acid with the Qiagen kit, RT-PCR analysed swabs for the Influenza A matrix gene. Sera samples were screened by Enzyme-Linked Immunosorbent Assay before subtyping a cross-section for H3 antibody by Hemagglutination Inhibition. No matrix gene was amplified from extracted nucleic acid from the nasal swabs. Though few sera were reactive to influenza A nucleoprotein, none was positive for influenza A/H3. The H3N8 strain of equine influenza virus first caused an epidemic in dogs in 1999 in the United States. Subsequently, avian-origin H3N2 CIV emerged in dogs in China and South Korea in 2005. Past CIV epidemics arose from a single cross-species transmission of H3N8 subtype from a mammalian intermediate host and the H3N2 subtype from an avian reservoir. Even though this limited investigation did not detect CIV in Plateau State, the potential remains because of the persistent circulation of avian, swine, and equine Influenza in Nigeria, which requires more extensive virological and serological surveillance.
{"title":"Serological and molecular investigation of canine influenza virus in Plateau State, Nigeria","authors":"DO Omoniwa, C. Chinyere, ER Agusi, N. Mkpuma, J. Oyetunde, OE Igah, Adole, A.M. Adidu-Omoniwa, C. Meseko","doi":"10.4314/sokjvs.v20i3.8","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.8","url":null,"abstract":"Canine influenza is a highly contagious respiratory infection of dogs caused by the Influenza A Virus (IAV), characterized by cough, sneeze, nasal secretions, and inappetence. Infections can be mild, severe or fatal. Aquatic birds constitute a natural reservoir for IAV, which is transmitted to terrestrial birds, including poultry. IAV has also emerged in other mammalian species, including humans, swine, horses, and dogs. IAV epidemics in dogs are a recent development. Commonly detected Canine Influenza Virus (CIV) strains are A/H3N2 and A/H3N8 from avian and equine influenza, respectively. Nigeria’s agro-ecology witnessed widespread circulation of avian influenza since 2006, and recent outbreaks of equine influenza in 2018/2019 raise the possibility of inter-species transmission to dogs. To investigate canine Influenza in Plateau State, we collected 113 nasal swabs and 270 sera samples from dogs in clinics, live dog markets, and during dog vaccination campaigns. After extracting nucleic acid with the Qiagen kit, RT-PCR analysed swabs for the Influenza A matrix gene. Sera samples were screened by Enzyme-Linked Immunosorbent Assay before subtyping a cross-section for H3 antibody by Hemagglutination Inhibition. No matrix gene was amplified from extracted nucleic acid from the nasal swabs. Though few sera were reactive to influenza A nucleoprotein, none was positive for influenza A/H3. The H3N8 strain of equine influenza virus first caused an epidemic in dogs in 1999 in the United States. Subsequently, avian-origin H3N2 CIV emerged in dogs in China and South Korea in 2005. Past CIV epidemics arose from a single cross-species transmission of H3N8 subtype from a mammalian intermediate host and the H3N2 subtype from an avian reservoir. Even though this limited investigation did not detect CIV in Plateau State, the potential remains because of the persistent circulation of avian, swine, and equine Influenza in Nigeria, which requires more extensive virological and serological surveillance.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126498658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Alaba, D. Omoniwa, E. Olajide, S. A. Koleosho, J.T. Olaleye
Parasitic diseases, which can reside inside or outside the host, have a devastating impact on human and animal health worldwide, particularly in developing countries. Little is known about the current haemoparasitic status of polo horses in Ibadan, Nigeria. This study aims to find out the scope and extent of haemoparasites and their associated haematological and serum biochemical changes in polo horses in Ibadan, Nigeria. Blood samples were randomly collected from 52 horses from Ibadan polo stable. Haemoparasites identification, haematology and serum biochemistry parameters were evaluated according to standard procedures. The overall prevalence of haemoparasitic infection in the sampled horses was 13.5%. The haemoparasites found were Theileria equi (85.7%) and Anaplasma phagocytophilum (14.3%). The haematology result showed that infected horses presented a significant decrease (P<0.05) in PCV, WBC counts and significant elevation (P<0.05) of eosinophils. The serum biochemical values revealed that infected horses had significantly higher (P<0.05) levels of AST and creatinine kinase whereas the glucose level was significantly lower (P<0.05) when compared with the uninfected horses. To reduce the threat of haemoparasitic infection, it is recommended that vector control, as well as prophylactic therapy, be carried out in stables.
{"title":"Prevalence of haemoparasites and influence on haemato-biochemical parameters of polo horses in Ibadan, Nigeria","authors":"B. Alaba, D. Omoniwa, E. Olajide, S. A. Koleosho, J.T. Olaleye","doi":"10.4314/sokjvs.v20i3.7","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.7","url":null,"abstract":"Parasitic diseases, which can reside inside or outside the host, have a devastating impact on human and animal health worldwide, particularly in developing countries. Little is known about the current haemoparasitic status of polo horses in Ibadan, Nigeria. This study aims to find out the scope and extent of haemoparasites and their associated haematological and serum biochemical changes in polo horses in Ibadan, Nigeria. Blood samples were randomly collected from 52 horses from Ibadan polo stable. Haemoparasites identification, haematology and serum biochemistry parameters were evaluated according to standard procedures. The overall prevalence of haemoparasitic infection in the sampled horses was 13.5%. The haemoparasites found were Theileria equi (85.7%) and Anaplasma phagocytophilum (14.3%). The haematology result showed that infected horses presented a significant decrease (P<0.05) in PCV, WBC counts and significant elevation (P<0.05) of eosinophils. The serum biochemical values revealed that infected horses had significantly higher (P<0.05) levels of AST and creatinine kinase whereas the glucose level was significantly lower (P<0.05) when compared with the uninfected horses. To reduce the threat of haemoparasitic infection, it is recommended that vector control, as well as prophylactic therapy, be carried out in stables.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"374 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133676997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Y. Gurumyen, E. Tizhe, T. N. Polycarp, L. Adekunle, A. Usman, T. Omadevuaye, M. N. Patrobas, E. S. Unanam, A. Jubril, R. Antia, V. Taiwo
Biliary cystadenoma is reported in an 8-year-old female Caucasian dog diagnosed by gross and microscopic examination. The gross lesion observed during postmortem examination on the liver was a 3×2cm cream-coloured raised nodule that extended into the parenchyma and another 2×4cm cyst filled with mucoid fluid. Histopathology showed multiloculated cysts lined by columnar and occasionally flattened epithelial cells, with papillary projections into the lumens. These findings are discussed in the paper.
{"title":"Biliary cystadenoma in an 8-year-old Caucasian bitch","authors":"G. Y. Gurumyen, E. Tizhe, T. N. Polycarp, L. Adekunle, A. Usman, T. Omadevuaye, M. N. Patrobas, E. S. Unanam, A. Jubril, R. Antia, V. Taiwo","doi":"10.4314/sokjvs.v20i3.9","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.9","url":null,"abstract":"Biliary cystadenoma is reported in an 8-year-old female Caucasian dog diagnosed by gross and microscopic examination. The gross lesion observed during postmortem examination on the liver was a 3×2cm cream-coloured raised nodule that extended into the parenchyma and another 2×4cm cyst filled with mucoid fluid. Histopathology showed multiloculated cysts lined by columnar and occasionally flattened epithelial cells, with papillary projections into the lumens. These findings are discussed in the paper.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131163614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mshelia, U. Ibrahim, S. U. Hassan, K. D. Malgwi, S. Jauro, N. Daniel
This study was carried out to determine the response of Japanese quails experimentally infected with Newcastle disease virus (NDV) kudu 113 strain using a haemagglutination inhibition test and the ability of the species to transmit the infection to chickens. The administration of kudu 113 strain of Newcastle disease virus (108.5 /ml) orally at 0.1ml/quail in the infected group (group B) resulted in an antibody response with a geometric mean titre of 23.79 on day 32 when compared to non-infected quails (group A) which did not show (p>0.05) evidence of Newcastle disease antibodies throughout the experiment and also differed significantly (p<0.05) from group B, indicating that oral inoculation of the virus was successful and the birds were infected. Clinical signs of ND were first observed in the quails 7 days post-infection (pi) with effects on egg production and egg quality. The transmission of the velogenic NDV from the quails (group B) to the sentinel chickens was clinically evident 4 days after they were placed in close contact with the infected quails. There was 100% mortality in the sentinel chickens between 4 to 7 days post contact. Thus, quails could serve as a potential source of ND for chickens.
{"title":"Susceptibility of Japanese quails (Cortunix cortunix japonica) to experimental infection with Newcastle disease virus, Kudu 113 strain","authors":"S. Mshelia, U. Ibrahim, S. U. Hassan, K. D. Malgwi, S. Jauro, N. Daniel","doi":"10.4314/sokjvs.v20i3.3","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.3","url":null,"abstract":"This study was carried out to determine the response of Japanese quails experimentally infected with Newcastle disease virus (NDV) kudu 113 strain using a haemagglutination inhibition test and the ability of the species to transmit the infection to chickens. The administration of kudu 113 strain of Newcastle disease virus (108.5 /ml) orally at 0.1ml/quail in the infected group (group B) resulted in an antibody response with a geometric mean titre of 23.79 on day 32 when compared to non-infected quails (group A) which did not show (p>0.05) evidence of Newcastle disease antibodies throughout the experiment and also differed significantly (p<0.05) from group B, indicating that oral inoculation of the virus was successful and the birds were infected. Clinical signs of ND were first observed in the quails 7 days post-infection (pi) with effects on egg production and egg quality. The transmission of the velogenic NDV from the quails (group B) to the sentinel chickens was clinically evident 4 days after they were placed in close contact with the infected quails. There was 100% mortality in the sentinel chickens between 4 to 7 days post contact. Thus, quails could serve as a potential source of ND for chickens.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123457000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Wajah, B. Emikpe, D. Asare, T. N. Asenso, D. Essel-Cobbinah
The objective of this study was to assess the preference for grasscutter offal among consumers in the Greater Accra and Ashanti regions of Ghana. The stratified three-stage random sampling was adopted for the selection of respondents. A total of 140 respondents (120 consumers; 20 operators) were used in this study. In the Ashanti region, 93 consumers and 13 chop bar operators were considered. Within the Greater Accra region, only one market was selected (Agbogbloshie market), where 27 consumers and seven chop bar operators were considered. Relevant information was collected through the administration of well-structured questionnaires. Results obtained showed a relatively higher offal preference by consumers in the Ashanti region (91.4%) as compared to the Greater Accra region (81.5%). Most consumers preferred the intestines and intestinal contents in both represented areas 60% from the Ashanti region and 45.6% from the Greater Accra region. 90.6% and 81.8% of the consumers from the Ashanti and Greater Accra regions, respectively preferred cooked offal to either smoked or roasted. These regional differences were not statistically significant. The rate of offal consumption was higher in the Ashanti region (i.e., on a weekly basis) as compared to the Greater Accra region (i.e., occasionally), represented by 63.5% and 68.2%, respectively. This difference was statistically significant (p<0.05). The taste was considered the most significant, relevant and important factor affecting consumer preference for grasscutter offal consumption in both regions. It is concluded in this study that the grasscutter offal has relatively high patronage by the respondents in this study. Further studies should be focused on the possible health implication of offal consumption on consumers as the intestines, and their contents might contain some parasites and other bacteria which can be harmful to humans. Further studies should be carried out to obtain a nationwide view of grasscutter offal consumption.
{"title":"Preference for grasscutter offal by some consumers in the Greater Accra and Ashanti regions of Ghana","authors":"A. Wajah, B. Emikpe, D. Asare, T. N. Asenso, D. Essel-Cobbinah","doi":"10.4314/sokjvs.v20i3.1","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.1","url":null,"abstract":"The objective of this study was to assess the preference for grasscutter offal among consumers in the Greater Accra and Ashanti regions of Ghana. The stratified three-stage random sampling was adopted for the selection of respondents. A total of 140 respondents (120 consumers; 20 operators) were used in this study. In the Ashanti region, 93 consumers and 13 chop bar operators were considered. Within the Greater Accra region, only one market was selected (Agbogbloshie market), where 27 consumers and seven chop bar operators were considered. Relevant information was collected through the administration of well-structured questionnaires. Results obtained showed a relatively higher offal preference by consumers in the Ashanti region (91.4%) as compared to the Greater Accra region (81.5%). Most consumers preferred the intestines and intestinal contents in both represented areas 60% from the Ashanti region and 45.6% from the Greater Accra region. 90.6% and 81.8% of the consumers from the Ashanti and Greater Accra regions, respectively preferred cooked offal to either smoked or roasted. These regional differences were not statistically significant. The rate of offal consumption was higher in the Ashanti region (i.e., on a weekly basis) as compared to the Greater Accra region (i.e., occasionally), represented by 63.5% and 68.2%, respectively. This difference was statistically significant (p<0.05). The taste was considered the most significant, relevant and important factor affecting consumer preference for grasscutter offal consumption in both regions. It is concluded in this study that the grasscutter offal has relatively high patronage by the respondents in this study. Further studies should be focused on the possible health implication of offal consumption on consumers as the intestines, and their contents might contain some parasites and other bacteria which can be harmful to humans. Further studies should be carried out to obtain a nationwide view of grasscutter offal consumption.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121044080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Olorunshola, Oluwafemi Babatunde Daodu, M. Ogunyemi, F. Folahan, S. Omoregie, J. Ogah
Newcastle disease (ND) is a disease of high economic importance to poultry farmers in Nigeria. Its impact on poultry include illness of poultry, reduction in egg production, immunosuppression, and death. This study was carried out to determine the prevalence of Newcastle disease in indigenous (local) chickens from 2 poultry abattoirs in the Ilorin metropolis. A total of 400 blood samples were aseptically collected in plain bottles from the jugular veins of local chickens at slaughter using exsanguination and transported to the laboratory in batches. Sera samples were harvested from the blood by centrifugation at 3000 rpm for 10 minutes, after which they were stored at -20oC before serological assay. The sera were subjected to Haemagglutination Inhibition (HI) test to check for the presence of Newcastle disease virus (NDV) antibody following a standard procedure with titer values for each sample recorded. The geometric mean of the HI antibody titer (GMT) and the percentages of detectable NDV HI antibody titer were calculated using descriptive statistics. Of the 400 serum samples examined, 53 (13.25%) were positive for ND antibodies, with titre value ≥ 1:16. The location from which the birds were selected had no significant relationship with the prevalence of ND antibodies as both Oja tuntun (11.9%), and Ipata market (14.6%) had a closely similar prevalence of antibodies (p > 0.05). The feather arrangement of birds did not also have any significant impact on the prevalence of antibodies (p > 0.05). However, in this study, we observed a higher prevalence of antibodies among hens (14.7%) than in cocks (12.5%) or growers (8.9%). The high prevalence of ND antibodies in indigenous chickens in the study area showed the endemicity of the disease in the study areas. With most of the chickens are not vaccinated amid non-compliance to vaccine administration for local chickens. There is a need for poultry farmers in the study location to be educated on the importance of vaccinating poultry birds against ND.
{"title":"Seroprevalence of Newcastle disease in indigenous chickens in Ilorin, Kwara State, Nigeria","authors":"I. Olorunshola, Oluwafemi Babatunde Daodu, M. Ogunyemi, F. Folahan, S. Omoregie, J. Ogah","doi":"10.4314/sokjvs.v20i3.4","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.4","url":null,"abstract":"Newcastle disease (ND) is a disease of high economic importance to poultry farmers in Nigeria. Its impact on poultry include illness of poultry, reduction in egg production, immunosuppression, and death. This study was carried out to determine the prevalence of Newcastle disease in indigenous (local) chickens from 2 poultry abattoirs in the Ilorin metropolis. A total of 400 blood samples were aseptically collected in plain bottles from the jugular veins of local chickens at slaughter using exsanguination and transported to the laboratory in batches. Sera samples were harvested from the blood by centrifugation at 3000 rpm for 10 minutes, after which they were stored at -20oC before serological assay. The sera were subjected to Haemagglutination Inhibition (HI) test to check for the presence of Newcastle disease virus (NDV) antibody following a standard procedure with titer values for each sample recorded. The geometric mean of the HI antibody titer (GMT) and the percentages of detectable NDV HI antibody titer were calculated using descriptive statistics. Of the 400 serum samples examined, 53 (13.25%) were positive for ND antibodies, with titre value ≥ 1:16. The location from which the birds were selected had no significant relationship with the prevalence of ND antibodies as both Oja tuntun (11.9%), and Ipata market (14.6%) had a closely similar prevalence of antibodies (p > 0.05). The feather arrangement of birds did not also have any significant impact on the prevalence of antibodies (p > 0.05). However, in this study, we observed a higher prevalence of antibodies among hens (14.7%) than in cocks (12.5%) or growers (8.9%). The high prevalence of ND antibodies in indigenous chickens in the study area showed the endemicity of the disease in the study areas. With most of the chickens are not vaccinated amid non-compliance to vaccine administration for local chickens. There is a need for poultry farmers in the study location to be educated on the importance of vaccinating poultry birds against ND.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123942357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Muhammad, Y. Garba, M. Bashir, N. Bello, S. Mohammed
This study evaluated the in vitro and in vivo free radical scavenging potentials of methanol extract of Ficus thonningii leaf and its liquid chromatography-mass spectrometer (LCMS) profiling. The qualitative phytochemical screening was carried out and the results show the presence of tannins, flavonoids, saponins glycoside and triterpenes. Antioxidant activities were carried out in vitro and in vivo and the reducing power shows a value of 0.53 ± 0.007 with a significant (p < 0.05) difference compared to the control and Diphenylpicrylhydrazyl (DPPH) value of 59.0 ± 0.007 with a significantly different (p < 0.05) compared to the control. However, the two assays are concentration-dependent. The in vivo analysis shows that lipid peroxidation, superoxide dismutase and catalase activities have values of 24.03± 8.04, 1.60± 0.03 and 0.003± 0.001 respectively. All the values are significantly different when compared to the control and are concentration-dependent. The LCMS results revealed the presence of some important metabolites; phenolic glycosides and quassin, which are potential molecules against free radicals. Therefore, Ficus thonningii leaf contains vital antioxidants and could be used in health management to boost the immune system or in drug development.
{"title":"Free radical scavenging potential of methanol extract of Ficus thonningii leave and liquid chromatography-mass spectrometer (LCMS) profiling","authors":"A. Muhammad, Y. Garba, M. Bashir, N. Bello, S. Mohammed","doi":"10.4314/sokjvs.v20i3.5","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.5","url":null,"abstract":"This study evaluated the in vitro and in vivo free radical scavenging potentials of methanol extract of Ficus thonningii leaf and its liquid chromatography-mass spectrometer (LCMS) profiling. The qualitative phytochemical screening was carried out and the results show the presence of tannins, flavonoids, saponins glycoside and triterpenes. Antioxidant activities were carried out in vitro and in vivo and the reducing power shows a value of 0.53 ± 0.007 with a significant (p < 0.05) difference compared to the control and Diphenylpicrylhydrazyl (DPPH) value of 59.0 ± 0.007 with a significantly different (p < 0.05) compared to the control. However, the two assays are concentration-dependent. The in vivo analysis shows that lipid peroxidation, superoxide dismutase and catalase activities have values of 24.03± 8.04, 1.60± 0.03 and 0.003± 0.001 respectively. All the values are significantly different when compared to the control and are concentration-dependent. The LCMS results revealed the presence of some important metabolites; phenolic glycosides and quassin, which are potential molecules against free radicals. Therefore, Ficus thonningii leaf contains vital antioxidants and could be used in health management to boost the immune system or in drug development.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"443 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133688614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Daniel, K. D. Malgwi, I. J. Omeh, E.N. Mkpokporo, P. Mshelia, Y.H. Mailabari, N. Ojo
Methanolic extract of Moringa oleifera flowers was investigated for its acute toxicity and effects on haematological parameters of Wistar rats. The acute toxicity study to determine the LD50 was evaluated using Lorke’s method. It involves first and second phases with six rats each phase. The rats were divided into three groups of two rats each of the phase. In the first, they were administered 10, 100 and 1000 mg/kg of the extract intraperitonially while in the second phase, they were administered 1600, 2900 and 5000 mg/kg and then observed for 24 hours. The haematological study was carried out according to standard procedures. Twenty (20) rats were divided into 4 groups with five rats in each group. The rats in groups B, C and D were treated orally with the methanolic extract of Moringa oleifera flowers using 200 mg/kg, 400 mg/kg, and 800 mg/kg respectively for 21 days while group A that served as control were administered sterile water for injection at 0.5 ml/kg. Blood sample was taken from all the rats and used to establish the baseline values prior to treatments with the extract. Blood samples were collected at the end of every week and analyzed for haematological values. The administration of the extract at the dose of 5000 mg/kg intraperitoneally to the rats resulted in death but was safe at the dose of 2900 mg/kg. This indicated that the LD50 is below 5000 mg/kg. There were significant increases (p<0.05) in red blood cells, haemoglobin concentration and the packed cell volume values in all treated groups when compared to the baseline values (control group). There were also significant (p<0.05) decrease and variation in the neutrophilic values and an increase in the lymphocytic values of the differential leukocytes counts. This research has revealed that the methanolic extract of Moringa oleifera flowers increased significantly the red blood cells, packed cell volume, haemoglobin concentration and lymphocytosis in Wistar rats.
{"title":"Acute toxicity and effects of methanolic extract of Moringa oleifera flower on haematological parameters of Wistar rats","authors":"N. Daniel, K. D. Malgwi, I. J. Omeh, E.N. Mkpokporo, P. Mshelia, Y.H. Mailabari, N. Ojo","doi":"10.4314/sokjvs.v20i3.2","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i3.2","url":null,"abstract":"Methanolic extract of Moringa oleifera flowers was investigated for its acute toxicity and effects on haematological parameters of Wistar rats. The acute toxicity study to determine the LD50 was evaluated using Lorke’s method. It involves first and second phases with six rats each phase. The rats were divided into three groups of two rats each of the phase. In the first, they were administered 10, 100 and 1000 mg/kg of the extract intraperitonially while in the second phase, they were administered 1600, 2900 and 5000 mg/kg and then observed for 24 hours. The haematological study was carried out according to standard procedures. Twenty (20) rats were divided into 4 groups with five rats in each group. The rats in groups B, C and D were treated orally with the methanolic extract of Moringa oleifera flowers using 200 mg/kg, 400 mg/kg, and 800 mg/kg respectively for 21 days while group A that served as control were administered sterile water for injection at 0.5 ml/kg. Blood sample was taken from all the rats and used to establish the baseline values prior to treatments with the extract. Blood samples were collected at the end of every week and analyzed for haematological values. The administration of the extract at the dose of 5000 mg/kg intraperitoneally to the rats resulted in death but was safe at the dose of 2900 mg/kg. This indicated that the LD50 is below 5000 mg/kg. There were significant increases (p<0.05) in red blood cells, haemoglobin concentration and the packed cell volume values in all treated groups when compared to the baseline values (control group). There were also significant (p<0.05) decrease and variation in the neutrophilic values and an increase in the lymphocytic values of the differential leukocytes counts. This research has revealed that the methanolic extract of Moringa oleifera flowers increased significantly the red blood cells, packed cell volume, haemoglobin concentration and lymphocytosis in Wistar rats.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114597446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FS Umar, SG Usman, M. Muhammed, S. Enam, M. Babashani, S. Adamu
Avian coccidiosis remains an economically important disease affecting the poultry industry worldwide. The dearth of information on haematological changes resulting from field cases of coccidiosis in commercial layer chickens necessitated this research. One hundred and twenty commercial layer chickens sampled from different farms in Zaria, diagnosed of coccidiosis at early, mid/peak and late production stages were used for this study. Results showed a significant (p < 0.05) decline in almost all haematological parameters (except for elevated MCV) of commercial layer chickens which were diagnosed of coccidiosis at the different production stages when compared to corresponding values of other apparently healthy layers that served as controls. The mean corpuscular volume (MCV) values in the layers diagnosed of coccidiosis at all production stages were significantly higher than the values obtained from the corresponding apparently healthy ones that served as control, whereas the MCHC showed significant decreases across all layer chickens diagnosed of coccidiosis at different production stages when compared to the corresponding values of the apparently healthy control layers. Thus, the erythrocytic indices of the layer chickens with coccidiosis showed the RBCs were macrocytic and hypochromic. On the basis of mean PCV, layer chickens at early production stage (PCV: 24.51 ± 3.17%) were most severely affected by coccidiosis, following closely by the layers at mid/peak production stage (PCV: 24.66 ± 1.64%). The highest mean WBC (19.15 ± 2.99 x109/L) was recorded in layers with coccidiosis at mid/peak production stage, which showed they were better in mounting inflammatory response when compared to the mean values of layers at early (14.92 ± 2.85 x109/L) and late (17.99 ± 2.70 x109/L) stages of production. In conclusion, coccidiosis in commercial layer chickens caused significant haematological alterations which could necessitate dietary supplementation to prevent occurrence of anaemia and decline in egg production.
{"title":"Comparison of haematological changes associated with coccidiosis in commercial layer chickens at different production stages in Zaria, Nigeria","authors":"FS Umar, SG Usman, M. Muhammed, S. Enam, M. Babashani, S. Adamu","doi":"10.4314/sokjvs.v20i5.20","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i5.20","url":null,"abstract":"Avian coccidiosis remains an economically important disease affecting the poultry industry worldwide. The dearth of information on haematological changes resulting from field cases of coccidiosis in commercial layer chickens necessitated this research. One hundred and twenty commercial layer chickens sampled from different farms in Zaria, diagnosed of coccidiosis at early, mid/peak and late production stages were used for this study. Results showed a significant (p < 0.05) decline in almost all haematological parameters (except for elevated MCV) of commercial layer chickens which were diagnosed of coccidiosis at the different production stages when compared to corresponding values of other apparently healthy layers that served as controls. The mean corpuscular volume (MCV) values in the layers diagnosed of coccidiosis at all production stages were significantly higher than the values obtained from the corresponding apparently healthy ones that served as control, whereas the MCHC showed significant decreases across all layer chickens diagnosed of coccidiosis at different production stages when compared to the corresponding values of the apparently healthy control layers. Thus, the erythrocytic indices of the layer chickens with coccidiosis showed the RBCs were macrocytic and hypochromic. On the basis of mean PCV, layer chickens at early production stage (PCV: 24.51 ± 3.17%) were most severely affected by coccidiosis, following closely by the layers at mid/peak production stage (PCV: 24.66 ± 1.64%). The highest mean WBC (19.15 ± 2.99 x109/L) was recorded in layers with coccidiosis at mid/peak production stage, which showed they were better in mounting inflammatory response when compared to the mean values of layers at early (14.92 ± 2.85 x109/L) and late (17.99 ± 2.70 x109/L) stages of production. In conclusion, coccidiosis in commercial layer chickens caused significant haematological alterations which could necessitate dietary supplementation to prevent occurrence of anaemia and decline in egg production.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115062234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aminu Ahmadu, M. Saulawa, A. Magaji, AI Musawa, K. Ibrahim, K. Ahmad
Cystic Echinococcosis, among other food animal zoonotic diseases, heralds a significant zoonotic threat to meat consumers due to the lack of stringent meat inspection policies and poor awareness of zoonoses by most livestock traders/butchers. Therefore, this study is aimed at determining the prevalence of hydatid cysts in camels slaughtered at katsina metropolitan abattoir, Katsina State, Nigeria. A total of 392 samples were collected over three months (November 2019 to January 2020), of which 129 samples were from males and 263 from females. Information about the age, sex and body condition of the slaughtered camels were recorded. The carcass of each camel was inspected carefully for the presence of Hydatid cysts, the organs infected, and the number of cysts present. The data were subjected to statistical analysis using the Chi-square test (χ2), and significant levels were determined at p<0.05 level for all the data, which revealed an overall prevalence of 14.0% (55/392). The prevalence rate of Hydatid cysts was 16.3% in females and 9.3% in males, while it is 16.8 and 5.3% in adult and young camels, respectively. We recommend a holistic epidemiological study on the prevalence of hydatid cysts in camels and more public enlightenment for effective control measures against the disease in Katsina State.
{"title":"Prevalence of hydatid cyst in camels slaughtered at Katsina metropolitan abattoir, Katsina State, Nigeria","authors":"Aminu Ahmadu, M. Saulawa, A. Magaji, AI Musawa, K. Ibrahim, K. Ahmad","doi":"10.4314/sokjvs.v20i5.18","DOIUrl":"https://doi.org/10.4314/sokjvs.v20i5.18","url":null,"abstract":"Cystic Echinococcosis, among other food animal zoonotic diseases, heralds a significant zoonotic threat to meat consumers due to the lack of stringent meat inspection policies and poor awareness of zoonoses by most livestock traders/butchers. Therefore, this study is aimed at determining the prevalence of hydatid cysts in camels slaughtered at katsina metropolitan abattoir, Katsina State, Nigeria. A total of 392 samples were collected over three months (November 2019 to January 2020), of which 129 samples were from males and 263 from females. Information about the age, sex and body condition of the slaughtered camels were recorded. The carcass of each camel was inspected carefully for the presence of Hydatid cysts, the organs infected, and the number of cysts present. The data were subjected to statistical analysis using the Chi-square test (χ2), and significant levels were determined at p<0.05 level for all the data, which revealed an overall prevalence of 14.0% (55/392). The prevalence rate of Hydatid cysts was 16.3% in females and 9.3% in males, while it is 16.8 and 5.3% in adult and young camels, respectively. We recommend a holistic epidemiological study on the prevalence of hydatid cysts in camels and more public enlightenment for effective control measures against the disease in Katsina State.","PeriodicalId":253894,"journal":{"name":"Sokoto Journal of Veterinary Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126671294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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