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Effects of Grandchildren’s Love Language Program on Depression among Thai Older Adults 外孙关爱语言项目对泰国老年人抑郁的影响
Pub Date : 2020-09-01 DOI: 10.48048/WJST.2020.4979
Saifon Aekwarangkoon, Naiyana Noonil
Depression in older adults has remained 1 of the major detrimental public health problems both worldwide and in Thailand. Among the myriad healing approaches, mental attachment on the basis of grandchildren’s love language is favorably perceived by Thai older adults with depression. The purpose of this quasi-experimental study was to evaluate the effects of grandchildren’s love language program on reducing depression among Thai older adults. Eighty Thai older adults with mild to moderate depression levels received the so-called “Love Language of Grandchildren Program” (n = 40) and usual care (n = 40). The Nine-Question Scale (9Q) and Hamilton Rating Scale for Depression (HRSD) were distributed at the pre- and post-treatment and at the 12 and 24-week of the follow-ups. The treatment effectiveness was evaluated by using Multivariate Analysis of Covariance (MANCOVA). The 9Q and HRSD scores revealed a significantly neutralized depression for those in love language of grandchildren program and, on the other hand, no decline was observed in the usual care’s participants. Love language of grandchildren program effectively reduced 9Q and HRSD symptoms of emotional, cognitive, behavioral, and physical conditions respectively. It was also more effective than the usual care in reducing mild to moderate symptoms of depression (p < 0.05). The findings provide better understanding on how grandchildren approach to prevent depression for Thai older adults in a family and community, and can be used to guide further capacity development for grandchildren who provide helping to this group.
老年人抑郁症仍然是全世界和泰国的主要有害公共卫生问题之一。在众多的治疗方法中,以孙子的爱的语言为基础的精神依恋被泰国老年抑郁症患者所接受。本拟实验研究的目的是评估孙子的爱的语言计划对减少泰国老年人抑郁的效果。80名患有轻度至中度抑郁症的泰国老年人接受了所谓的“孙辈爱的语言计划”(n = 40)和常规护理(n = 40)。9题抑郁量表(9Q)和汉密尔顿抑郁评定量表(HRSD)分别于治疗前和治疗后以及随访12周和24周发放。采用多变量协方差分析(MANCOVA)评价治疗效果。9Q和HRSD分数显示,参加孙辈爱语项目的参与者的抑郁情绪得到了显著的缓解,而另一方面,在常规护理的参与者中没有观察到抑郁情绪的下降。孙辈爱语项目有效降低了9Q和HRSD在情绪、认知、行为和身体方面的症状。在减轻轻至中度抑郁症状方面也比常规护理更有效(p < 0.05)。这一发现有助于更好地理解在家庭和社区中,孙辈是如何预防泰国老年人抑郁的,并可用于指导为这一群体提供帮助的孙辈的进一步能力发展。
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引用次数: 1
Diagnosis of Dieback Disease of the Nutmeg Tree in Aceh Selatan, Indonesia 印度尼西亚亚齐塞拉坦地区肉豆蔻树枯死病的诊断
Pub Date : 2020-08-01 DOI: 10.48048/wjst.2020.4379
S. Susanna, M. S. Sinaga, S. Wiyono, H. Triwidodo
Effective control disease measures require a great deal of knowledge about the pathogen, which is also applied to dieback disease of the nutmeg tree in Aceh Selatan District, Province of Aceh, Indonesia. The objectives of this study were to identify the causal agent of dieback disease on a nutmeg plantation and to assess the effect of drought stress on the development of dieback disease. Pathogen identification was carried out by observing the symptoms of infected plants, morphological characters of the isolated pathogens, Koch’s postulates, and molecular analysis and pure pathogen cultures. The samples were taken from an infected part of the nutmeg plant (twigs, stems, and roots) in 6 subdistricts at the center of the nutmeg plantation in Aceh Selatan. Koch's postulates were performed by using the mycelia of pathogens on 1-year-old nutmeg seedlings. Drought stress was simulated everyday, and then once every 2 weeks, to study the effect of drought stress on dieback. The results showed that there were 4 genera of fungal pathogens (Botryodiplodia theobromae, Fusarium solani, Graphium euwallaceae, and Rigidoporus microporus) out of 6 fungi associated with dieback disease. However, B. theobromae Pat. turned out to be the dominant fungi associated with dieback on the nutmeg tree in Aceh Selatan. Drought stress was proven to be a predisposing factor for explaining this problem epidemic in Aceh Selatan.
有效的疾病控制措施需要对病原体有大量的了解,这也适用于印度尼西亚亚齐省亚齐塞拉坦地区肉豆蔻树的枯死病。本研究的目的是确定肉豆蔻人工林枯死病的致病因子,并评估干旱胁迫对肉豆蔻枯死病发展的影响。通过观察侵染植株的症状、分离病原菌的形态特征、科赫假设、分子分析和纯病原菌培养进行病原鉴定。样本取自亚齐塞拉坦肉豆蔻种植园中心6个街道的肉豆蔻植物感染部分(细枝、茎和根)。科赫的假设是通过在1年生的肉豆蔻幼苗上使用病原体的菌丝来实现的。每天模拟干旱胁迫,然后每2周模拟1次,研究干旱胁迫对枯枝的影响。结果表明,与枯死病相关的6种真菌病原菌中有4属(Botryodiplodia theobromae、Fusarium solani、grapium euwallaceae和Rigidoporus microporus)。然而,B. theobromae Pat。结果证明是亚齐塞拉坦肉豆蔻树枯死相关的优势真菌。干旱胁迫已被证明是解释亚齐塞拉坦地区这一问题流行病的一个诱发因素。
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引用次数: 4
The Effects of a Resilience Enhancing Nursing Program on Depression among Pregnant Teenagers 心理弹性增强护理方案对怀孕少女抑郁的影响
Pub Date : 2020-08-01 DOI: 10.48048/wjst.2020.3630
Umaporn Kuasit, S. Chunuan, U. Hatthakit, L. Bullock
The purposes of this study were to evaluate the effects of the resilience enhancing nursing program (RENP) on resilience and depression among pregnant teenagers who received this program. A quasi experimental was conducted with 130 pregnant Thai women. Final analysis included 130 participants (Intervention n = 64 and control group n = 66). The RENP was delivered in three steps including; Step 1, establishing trusting relationships; Step 2, improving the resilience, and Step 3, monitoring and encouraging the resilience practice. The research instruments consisted of a demographic data questionnaire, and resilience and depressive program instruments, including a handbook and self-report. Research data were analyzed and displayed, providing percentage, mean, standard deviation, chi-square, independent t-test, paired t-test, and the repeated measures ANOVA.The findings showed a significant difference in depression by time alone (p = 0.001). However, there was no significant difference by group alone in depression (p = 0.969). This study revealed mean score on depression in the experimental group was not significantly lower than before entering the program at week 4 and week 8 (p = 0.870,  p = 0.428). However, resilience showed difference in score over time between the experimental group and control group. There was a significant difference in resilience score by time alone (F = 10.523, p < 0.001).The results can contribute to guiding nurses to enhance decrease in depression in pregnant teenagers in the earlier first trimester in antenatal care units. Nurse or nurse midwife should be concerned about the potential for early detection in the prenatal period and provide appropriate interventions to teenage mothers in the postnatal period.
本研究的目的是评估心理弹性增强护理计划(RENP)对怀孕少女心理弹性和抑郁的影响。对130名泰国孕妇进行了准实验。最终分析纳入130名参与者(干预组64名,对照组66名)。RENP分三个步骤交付,包括:第一步,建立信任关系;第二步,提高弹性,第三步,监督和鼓励弹性实践。研究工具包括人口统计数据问卷、心理韧性和抑郁项目工具,包括一本手册和自我报告。对研究数据进行分析和显示,提供百分比、平均值、标准差、卡方、独立t检验、配对t检验和重复测量方差分析。研究结果显示,单独时间对抑郁的影响有显著差异(p = 0.001)。两组在抑郁方面差异无统计学意义(p = 0.969)。本研究发现,实验组的抑郁平均得分在第4周和第8周均未显著低于入组前(p = 0.870, p = 0.428)。然而,实验组和对照组的心理弹性随时间的变化表现出差异。时间因素对心理弹性评分有显著影响(F = 10.523, p < 0.001)。研究结果有助于指导护士在产前护理部门加强减少怀孕少女早期抑郁的工作。护士或助产士应关注产前早期发现的可能性,并在产后为少女母亲提供适当的干预措施。
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引用次数: 2
Quality Assessment Criteria for Mobile Health Apps: A Systematic Review 移动健康应用程序的质量评估标准:系统回顾
Pub Date : 2020-07-15 DOI: 10.48048/WJST.2020.6482
Wilasinee Hongsanun, S. Insuk
Summative evidences on the criteria for user assessment of health apps are still limited. In order to determine an assessment method for mobile health apps, rigorous and appropriate criteria must be chosen. The aim of this study is to review the existing quality assessment criteria that are being used to assess the quality of mobile health apps focusing on users’ perspectives. In addition, a procedure for the quality assessment of health apps is also presented. A systematic review was conducted utilizing 5 databases; PubMed, ScienceDirect, Scopus, CENTRAL and IEEE Xplore from 2008 to 2017. Search terms were; “mHealth”, “Applications”, “iOS OR Android”, “smartphones”, “quality”, etc. Papers that assessed the quality of health apps by users were selected. The PRISMA guideline was followed to complete the list of final studies. Likewise, a data extraction form was based on the PICO framework. From 6,514 studies, 69 studies met the eligibility criteria. Four hundred and forty-one quality assessment criteria for users were classified into 15 domains: Ease of use, Usability, Aesthetics, Connectivity, Functionality, Information, User satisfaction, Acceptability, Error occurrence, Motivation, Engagement, Data management, Undesirable events, Credentials and Security and privacy. An “ease of use” domain was found common in the included studies. The 15 domains were suggested as criteria for further study of the quality assessment and development of mHealth apps. Moreover, five recommended steps were presented for future research of quality of mobile health apps.
关于健康应用的用户评价标准的总结性证据仍然有限。为了确定移动健康应用程序的评估方法,必须选择严格和适当的标准。本研究的目的是回顾现有的质量评估标准,这些标准被用于评估关注用户观点的移动健康应用程序的质量。此外,还提出了一种健康应用程序的质量评估程序。利用5个数据库进行系统评价;PubMed, ScienceDirect, Scopus, CENTRAL和IEEE explore从2008年到2017年。搜索词是;“移动健康”、“应用”、“iOS或Android”、“智能手机”、“质量”等。选择了评估用户健康应用程序质量的论文。遵循PRISMA指南完成最终研究清单。同样,数据提取表单也是基于PICO框架的。从6514项研究中,69项研究符合入选标准。441项用户质量评估标准被分为15个领域:易用性、可用性、美观性、连接性、功能、信息、用户满意度、可接受性、错误发生率、动机、参与度、数据管理、不良事件、凭据、安全和隐私。在纳入的研究中发现“易用性”域是常见的。这15个领域被建议作为进一步研究移动健康应用程序质量评估和开发的标准。并对未来移动健康应用质量研究提出了5个建议步骤。
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引用次数: 4
Acute Toxicity of Cypermethrin on Nile tilapia Fry 氯氰菊酯对尼罗罗非鱼苗的急性毒性研究
Pub Date : 2020-07-01 DOI: 10.48048/wjst.2020.3815
Anan Kenthao, W. Sirisarn, P. Jearranaiprepame
The present study aimed to evaluate the acute toxicity effect of cypermethrin contaminated aquatic ecosystems by using a 30 days post-hatching fry of Nile tilapia as a test model. The control and six test experiments, each comprising 10 animals, were repeated three times and evaluated with the static test method. The lethal concentrations at 50 % (LC50) and 70 % (LC70) for 96 h were determined using the probit analysis. Behavioural and histological changes were observed in fish exposed with cypermethrin at both 96 h LC50 and 96 h LC70. The values of 96 h LC50 and 96 h LC70 were estimated at 32.496 and 40.311 ppb, respectively. The affected fish exhibited the loss of equilibrium with erratic and darting swimming movements, hyperactivity, secretion of mucous and increasing rate of opercula activity. Severity of histopathological lesions were related to concentration levels and exposure times. The histological changes of gill tissues included the swelling of epithelial cells and the fusion of secondary lamella. An enlargement of sinusoids, pyknotic nuclei, vacuole formation and degeneration in hepatic parenchyma were observed in liver. The degeneration of glomerulus combining with oedema of renal tubule was also noticed in kidney. No alter lesion was seen on skin tissue. The results in the present study suggest that low levels of cypermethrin in the aquatic environment may alter adverse effect on growth and development in Nile tilapia.
本研究以尼罗罗非鱼鱼苗为试验模型,评价氯氰菊酯污染水生生态系统的急性毒性效应。对照实验和6个试验,每组10只动物,重复3次,采用静态试验法进行评价。采用概率分析法测定50% (LC50)和70% (LC70)作用96 h的致死浓度。在96 h LC50和96 h LC70时,观察了氯氰菊酯暴露鱼的行为和组织学变化。96 h LC50和96 h LC70分别为32.496和40.311 ppb。受影响的鱼表现出不平衡的丧失,游动运动不稳定,游动速度快,过度活跃,粘液分泌和包盖活动率增加。组织病理学病变的严重程度与浓度水平和暴露时间有关。鳃组织的组织学变化包括上皮细胞肿胀和继发片层融合。肝内可见肝窦增大、核固缩、空泡形成及肝实质变性。肾脏可见肾小球变性合并肾小管水肿。皮肤组织未见其他病变。本研究结果表明,低水平的氯氰菊酯可能会改变尼罗罗非鱼生长发育的不利影响。
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引用次数: 1
Physico-Chemical Characteristics of Giant Featherback (Chitala lopis) and Mackerel (Scomberomorus commerson) Bone Gelatins and Their Potential as Gelling agents in Gel Mixtures for Roselle (Hibiscus sabdariffa L.) Soft Jelly Candies 巨型羽背(Chitala lopis)和鲭鱼(Scomberomorus commerson)骨明胶的理化特性及其在玫瑰茄凝胶混合物中用作胶凝剂的潜力软糖
Pub Date : 2020-07-01 DOI: 10.48048/wjst.2020.4559
Y. Yuliani, Bambang Tri Agung, M. Marwati, K. P. Candra
The gel strength of gelatin depends on its raw material. Fish gelatin shows a broad range of gel strengths. Giant featherback and mackerel bone gelatins have the gel strengths 220 and 38 Bloom, respectively. Here, we prepared roselle soft jelly candy using the single gelatin and mixtures gelatins from bovine, giant featherback, and mackerel sources. Roselle calyces extract has been used as a food additive to improve taste, aroma, and antioxidant content. The soft jelly candies (1.5 % w/v roselle extract and 12 % gelling agent) were prepared using three types of gelatin (giant featherback (GF), mackerel (M), and bovine (B)) in four mixtures (GF:B = 1:3, GF:B = 1:1, M:B = 1:3, and M:B = 1:1). This experiment was arranged in a completely randomized design with three replications for each treatment. Physico-chemical and sensory characteristics of the jelly candies were determined. The data were analyzed by ANOVA continued by the LSD test. The results showed that water content, pH, gel strength, hardness, rigidity, and hedonic quality (taste, color, texture, and overall performance) of the roselle soft jelly candies were affected by choice of gelatin, but not total reducing sugar. Roselle soft jelly candy made with giant featherback and bovine gelatin (1:3) mix was the most preferred by the panelists.
明胶的凝胶强度取决于它的原料。鱼明胶显示出广泛的凝胶强度。巨型羽背胶和鲭鱼骨胶的凝胶强度分别为220和38 Bloom。在这里,我们使用单一明胶和混合明胶从牛,巨型羽毛和鲭鱼来源制备玫瑰软果冻糖。玫瑰花萼提取物已被用作食品添加剂,以改善口感,香气和抗氧化剂的含量。以巨羽毛胶(GF)、鲭鱼胶(M)、牛胶(B)三种明胶(GF:B = 1:3、GF:B = 1:1、M:B = 1:3、M:B = 1:1)为原料,制备软果冻糖(1.5% w/v玫瑰叶提取物和12%胶凝剂)。本试验采用完全随机设计,每个处理3个重复。测定了果冻糖的理化特性和感官特性。数据分析采用方差分析,继续采用LSD检验。结果表明,明胶的选择对玫瑰软果冻糖的含水量、pH值、凝胶强度、硬度、刚性和口感、颜色、质地和综合性能有影响,但对总还原糖没有影响。由巨型羽毛和牛明胶(1:3)混合制成的Roselle软糖是小组成员最喜欢的。
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引用次数: 0
Prediction of Rheological Properties and Interfacial Tension of Mixtures of Immiscible Polypropylene-Polystyrene (PP3/PS) Blends 聚丙烯-聚苯乙烯(PP3/PS)共混物流变性能和界面张力的预测
Pub Date : 2020-07-01 DOI: 10.48048/wjst.2020.3294
Meriem Boudoukhani, N. Moulai-Mostefa, S. Hammani
Blends of polypropylene (PP3) and polystyrene (PS) were studied and, their rheological behavior was determined and discussed in detail. The interfacial tension between the blend components was evaluated from the rheological data and the storage modulus by using two well-known models: Palierne model and Choi-Schowalter equation. The theoretical predictions were compared with experimental data obtained from PP3/PS blends. The obtained results showed that the Palierne model could predict the rheological and viscoelastic properties of the considered polymer blends. In addition, the interfacial tensions between PP3 and PS were evaluated and compared with those cited in the literature. It was also found that the Palierne model was more accurate than Choi-Schowalter one in determining the interfacial tension.
研究了聚丙烯(PP3)和聚苯乙烯(PS)的共混物,并对其流变行为进行了详细的测定和讨论。利用Palierne模型和Choi-Schowalter方程,从流变数据和存储模量两方面对共混组分之间的界面张力进行了计算。将理论预测结果与PP3/PS共混物的实验数据进行了比较。得到的结果表明,Palierne模型可以预测所考虑的聚合物共混物的流变和粘弹性。此外,我们还评估了PP3和PS之间的界面张力,并与文献中引用的界面张力进行了比较。在确定界面张力方面,Palierne模型比Choi-Schowalter模型更准确。
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引用次数: 1
Theses and Capstone Projects Plagiarism Checker using Kolmogorov Complexity Algorithm 基于Kolmogorov复杂度算法的论文和论文抄袭检查
Pub Date : 2020-07-01 DOI: 10.14456/VOL17ISS3PP%P
Marco Jr. Nañadiego Del Rosario, Julius A Sareno
In education, students attempt to copy previous works and are relying on prepared solutions available on the Internet in order to meet their requirements. This action leads to plagiarism, which is becoming part of educational institutions’ concern to reduce growing academic dishonesty. With regards to the aforementioned issue, this study aims to design and develop a plagiarism checker capable of registering documents, granting access to users, and calculating the similarity between documents. Thus, the software was constructed using HTML, PHP, JavaScript, CSS, and MySQL. The developed system is composed of three main modules; the Document Search which enables users to browse documents, the Document Registration which enables the administrator to add and manage the stored documents, and the document Comparison, which serves as the system plagiarism detection mechanism. The algorithm Normalized Compression Distance was used to measure similarity and the Boyer-Moore Algorithm to highlight the suspected plagiarized document. Moreover, tests were conducted to determine if the system is functioning as expected and to measure the accuracy of the output produced by the system. The developed system was evaluated using the ISO 25010 software quality model in terms of Product Quality and was rated by one hundred respondents. The system obtained a mean of 4.70 which is equivalent to “excellent” in descriptive terms. This validates that the objectives of the study were met and achieved. This further indicates that the system was developed according to its desired functions and requirements.
在教育中,学生试图复制以前的作品,并依靠互联网上现成的解决方案来满足他们的要求。这种行为导致了剽窃,这成为教育机构关注的一部分,以减少日益增长的学术不诚实。针对上述问题,本研究旨在设计和开发一个能够注册文档,授予用户访问权限,并计算文档之间相似度的抄袭检查器。因此,该软件是使用HTML, PHP, JavaScript, CSS和MySQL构建的。开发的系统由三个主要模块组成;文档搜索,用户可以浏览文档;文档注册,管理员可以对存储的文档进行添加和管理;文档比对,作为系统的抄袭检测机制。采用归一化压缩距离算法来衡量相似度,采用Boyer-Moore算法来突出疑似抄袭的文档。此外,还进行了测试,以确定系统是否按预期运行,并测量系统产生的输出的准确性。开发的系统在产品质量方面使用ISO 25010软件质量模型进行评估,并由100名受访者进行评级。该系统的平均得分为4.70,在描述性术语中相当于“优秀”。这证实了研究的目标得到满足和实现。这进一步表明,该系统是按照预期的功能和要求开发的。
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引用次数: 2
Prevalence and Characterization of Pathogenic Bacteria in Bulk Tank Raw Milk, Thailand 泰国散装罐原料奶中致病菌的流行和特征
Pub Date : 2020-06-01 DOI: 10.48048/wjst.2020.4177
Chanida Kupradit, Sasidhorn Innok, Jirayus Woraratphoka, M. Ketudat-Cairns
Although there are a number of raw milk collection centers in Nakhon Ratchasima, there is a lack of information with regard to the process of isolation and characterization of foodborne pathogens in raw milk. Therefore, the purpose of this research was to investigate the prevalence and characterization of foodborne pathogens, including Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus from 33 raw milk samples from 9 different raw milk collection centers located in 8 districts in Nakhon Ratchasima, Thailand. This study was conducted from January to March 2016. Results revealed that the contaminations of L. monocytogenes and Salmonella spp. were not detected in any of the raw milk samples tested. The prevalence of B. cereus, E. coli, and S. aureus in raw milk samples was found to be 9 % (10 - 2.0 ×104 CFU/ml), 42.4 and 54 % (85 - 2.7 ×104 CFU/ml), respectively. The distribution of virulence genes was tested in B. cereus and S. aureus using gene specific primers by polymerase chain reaction. Out of the 29 analyzed coagulase-positive S. aureus isolates, 27 isolates (93 %) were positive for eap gene amplification and 14 isolates (48 %) showed amplicon of eap gene and all 5 enterotoxin genes, including seG, seGV, seI, seIV, and seM genes. All 8 B. cereus isolates tested showed positive PCR result with enterotoxin FM (entFM) gene but they showed negative with hemolysin gene (hblA and hblD genes) amplifications. It was inferred from these findings that bulk tank milk is a potential source of S. aureus and B. cereus in milk.
虽然在那空叻差玛有一些原料奶收集中心,但缺乏关于原料奶中食源性病原体的分离和表征过程的信息。因此,本研究的目的是调查泰国那空府8个区9个不同原料奶采集中心的33份原料奶样品中蜡样芽孢杆菌、大肠杆菌、单核细胞增生李斯特菌、沙门氏菌和金黄色葡萄球菌等食源性致病菌的流行情况和特征。本研究于2016年1月至3月进行。结果显示,所有原料奶样品均未检出单核增生乳杆菌和沙门氏菌污染。原料奶样品中蜡样芽孢杆菌、大肠杆菌和金黄色葡萄球菌的检出率分别为9% (10 ~ 2.0 ×104 CFU/ml)、42.4和54% (85 ~ 2.7 ×104 CFU/ml)。采用聚合酶链反应技术,利用基因特异性引物检测蜡样芽孢杆菌和金黄色葡萄球菌毒力基因的分布。在29株凝固酶阳性金黄色葡萄球菌中,27株(93%)eap基因扩增阳性,14株(48%)eap基因和5个肠毒素基因(包括seG、seGV、seI、seIV和seM基因)均扩增。8株蜡样芽孢杆菌分离株肠毒素FM (entFM)基因PCR结果均为阳性,溶血素基因hblA和hblD基因PCR结果均为阴性。从这些发现推断,散装罐牛奶是牛奶中金黄色葡萄球菌和蜡样芽孢杆菌的潜在来源。
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引用次数: 3
Effect of Cyanobacteria (Nostoc species) Extracts on Osteogenesis Activities 蓝藻(Nostoc)提取物对成骨活性的影响
Pub Date : 2020-06-01 DOI: 10.48048/wjst.2020.5350
Phatthilakorn Chamnanpuen, Veena Satitpatipan, S. Sirisattha, T. Muangman
This study focused on osteogenesis activities from Cyanobacteria (Nostoc species), including cytotoxicity, cell proliferation, cell differentiation and cell mineralization with osteoblast cell (MC3T3-E1). The four Nostoc species; N. parmelioides, N punctiforme, N. muscorum and N. paludosum were made axenic and cultured for a month. The ethanolic crude extracts were obtained by maceration extraction; the yield was in the range of 7.00 - 10.00 % (w/w). By using the TLC technique, it was observed that in all of the samples, there were phytochemical bands corresponding with β-carotene and chlorophyll a which were used as standard compounds. As a result, all crude extracts had no toxic effect on osteoblast cells after being incubated for 24 and 48 h with various concentrations (1 - 1,000 µg/mL), although they did not significantly promote osteoblast proliferation. In addition, it was found that 3 crude extracts (N. parmelioides, N punctiforme, and N. paludosum) also promoted osteoblast differentiation significantly after being induced for 9 days. Similarly, there was no difference between the relative cellular mineralization among treatment groups after being induced for 25 days. The potential ethanolic extracts on osteoblast cell differentiation were further partitioned by liquid-liquid partition chromatography (hexane, dichloromethane and aqueous fractions), and were analyzed for osteoblast differentiation with alkaline phosphatase (ALP) activity. All of hexane and dichloromethane fractions from N. parmelioides (181.72±6.75 % and 195.11±3.27 %), N punctiforme (194.34±2.36 % and 168.69±1.08 %), and N. paludosum (191.54±7.66 % and 167.12±9.46 %) had a significantly higher effect of ALP activity than their aqueous fractions. In conclusion, our data suggested that the nonpolar potential major compound, which might correspond with carotenoids (β-carotene, xanthophyll and chlorophyll a) from Nostoc species, exhibited positive effects on osteoblast differentiation by increasing the alkaline phosphatase enzyme.
本研究主要研究蓝藻(Nostoc)的成骨活性,包括细胞毒性、细胞增殖、细胞分化和成骨细胞(MC3T3-E1)矿化。四种Nostoc;将无性虫、点状无性虫、白色无性虫和白色无性虫无菌培养1个月。浸渍法提取乙醇粗提物;产率在7.00 ~ 10.00 % (w/w)之间。通过薄层色谱技术观察到,所有样品中均存在与β-胡萝卜素和叶绿素a对应的植物化学谱带,并将其作为标准化合物。结果表明,不同浓度(1 - 1000µg/mL)培养24和48 h后,所有粗提物对成骨细胞均无毒性作用,但对成骨细胞增殖无显著促进作用。此外,我们发现3种粗提物(parmelioides, N. punctiforme, N. paludosum)在诱导9天后也能显著促进成骨细胞分化。同样,在诱导25天后,各处理组之间的相对细胞矿化没有差异。利用液-液分配层析(正己烷、二氯甲烷和水相)对成骨细胞分化的乙醇提取物进行进一步分离,并利用碱性磷酸酶(ALP)活性对成骨细胞分化进行分析。其中,正己烷和二氯甲烷组分(分别为181.72±6.75%和195.11±3.27%)、刺状氮(分别为194.34±2.36%和168.69±1.08%)和白花氮(分别为191.54±7.66%和167.12±9.46%)对ALP活性的影响均显著高于水相组分。综上所述,我们的数据表明,非极性电位主要化合物可能与Nostoc物种中的类胡萝卜素(β-胡萝卜素、叶黄素和叶绿素a)相对应,通过增加碱性磷酸酶对成骨细胞分化有积极的影响。
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引用次数: 1
期刊
Walailak Journal of Science and Technology (WJST)
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