A. Saimmai, Wiboon Riansa-ngawong, S. Maneerat, P. Dikit
It is generally known that both chemical substances and many kinds of microorganism can be used to produce surfactants or surface-active compounds. Surfactants derived from microorganisms are called biosurfactants, or bio-surface active compounds. Recently, biosurfactants have become more interesting because of their advantages, such as less toxicity and more degradability, which cannot be found in traditional surfactants. Biosurfactant production faces some problems, such as a high cost of production. In the medical field, biosurfactants are attractive, because the products from biosurfactants can be used effectively in small amounts. This can compensate for the high cost of production. In addition, there have been many great discoveries of biosurfactants in the medical field.
{"title":"Application of Biosurfactants in the Medical Field","authors":"A. Saimmai, Wiboon Riansa-ngawong, S. Maneerat, P. Dikit","doi":"10.48048/WJST.2020.4748","DOIUrl":"https://doi.org/10.48048/WJST.2020.4748","url":null,"abstract":"It is generally known that both chemical substances and many kinds of microorganism can be used to produce surfactants or surface-active compounds. Surfactants derived from microorganisms are called biosurfactants, or bio-surface active compounds. Recently, biosurfactants have become more interesting because of their advantages, such as less toxicity and more degradability, which cannot be found in traditional surfactants. Biosurfactant production faces some problems, such as a high cost of production. In the medical field, biosurfactants are attractive, because the products from biosurfactants can be used effectively in small amounts. This can compensate for the high cost of production. In addition, there have been many great discoveries of biosurfactants in the medical field.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130209325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-29DOI: 10.14456/VOL16ISS9PPCORRECTED
Pratchayaporn Doemlim, V. Laohakosol, Janyarak Tongsomporn
In 1954, Perron constructed simple continued fractions of e1/k and e2/k where k is a positive integer. These are called Hurwitz continued fractions. Using the method given in Perron’s book, we determine explicit shapes of simple continued fractions of ke1/k; 1 k e1/k and 2e.
{"title":"The Continued Fractions of Certain Exponentials","authors":"Pratchayaporn Doemlim, V. Laohakosol, Janyarak Tongsomporn","doi":"10.14456/VOL16ISS9PPCORRECTED","DOIUrl":"https://doi.org/10.14456/VOL16ISS9PPCORRECTED","url":null,"abstract":"In 1954, Perron constructed simple continued fractions of e1/k and e2/k where k is a positive integer. These are called Hurwitz continued fractions. Using the method given in Perron’s book, we determine explicit shapes of simple continued fractions of ke1/k; 1 k e1/k and 2e.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114746793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A brief account of our recent works dealing with the problem of characterizing hyperbolic functions through the use of functional equations with side conditions is reported. Specifically, the fact that each of the n functions having a zero sum over n points lying in a hyperplane is necessarily a linear function, is generalized and the results so obtained are applied to characterize hyperbolic functions.
{"title":"Characterizing Hyperbolic Functions over a Hyperplane","authors":"Kanet Ponpetch","doi":"10.48048/WJST.2019.6955","DOIUrl":"https://doi.org/10.48048/WJST.2019.6955","url":null,"abstract":"A brief account of our recent works dealing with the problem of characterizing hyperbolic functions through the use of functional equations with side conditions is reported. Specifically, the fact that each of the n functions having a zero sum over n points lying in a hyperplane is necessarily a linear function, is generalized and the results so obtained are applied to characterize hyperbolic functions.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126491801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although antibiotic has been frequently used for the treatment of infection, it has led to the emergence of resistant problem. Plant-derived compounds are alternative source for discovering novel therapeutics. Flavonoid is widely distributed and present in plant kingdom. This compound possessed several pharmacological properties including antibacterial. This review aims to present some information about the potency of flavonoids as antibacterial compound including their mechanism of antibacterial action as well as the relationship between their activity and flavonoid structure. The synergistic effect of flavonoids when used in combination with antibiotics against resistant bacterial is also described. Published literatures were collected from data bases such as PubMed, Google Scholar, Science Direct and Scopus. Scientific papers were selected based on information of antibacterial activity of flavonoid compounds. The information may provide an insight on the potency of flavonoid compounds to overcome resistant problem.
{"title":"Prospect of Plant-based Flavonoids to Overcome Antibacterial Resistance: A Mini-Review","authors":"A. Septama, N. Simbak, E. Rahmi","doi":"10.48048/wjst.2020.5583","DOIUrl":"https://doi.org/10.48048/wjst.2020.5583","url":null,"abstract":"Although antibiotic has been frequently used for the treatment of infection, it has led to the emergence of resistant problem. Plant-derived compounds are alternative source for discovering novel therapeutics. Flavonoid is widely distributed and present in plant kingdom. This compound possessed several pharmacological properties including antibacterial. This review aims to present some information about the potency of flavonoids as antibacterial compound including their mechanism of antibacterial action as well as the relationship between their activity and flavonoid structure. The synergistic effect of flavonoids when used in combination with antibiotics against resistant bacterial is also described. Published literatures were collected from data bases such as PubMed, Google Scholar, Science Direct and Scopus. Scientific papers were selected based on information of antibacterial activity of flavonoid compounds. The information may provide an insight on the potency of flavonoid compounds to overcome resistant problem.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133022157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To discover novel triazole-based antimicrobial agents, design and synthesis of acid chlorides possessing a 1,2,4-triazole core was reported in this journal in 2018. However, we found that the reported spectroscopic data did not show any agreement with the proposed acid chloride structure. Herein, we described our opinion, which was supported by IR data, circumstantial evidences, and mechanistic insight, that the products obtained in the topical paper would be thioesters.
{"title":"Opinion to: Design, Synthesis, Antimicrobial, and Antioxidant Activities of Novel {4,5-(substituted diphenyl)-4H-1,2,4-triazol-3-ylthio}acetyl Chloride","authors":"Khamid Khodjaniyazov, Kohei Torikai","doi":"10.48048/wjst.2019.6414","DOIUrl":"https://doi.org/10.48048/wjst.2019.6414","url":null,"abstract":"To discover novel triazole-based antimicrobial agents, design and synthesis of acid chlorides possessing a 1,2,4-triazole core was reported in this journal in 2018. However, we found that the reported spectroscopic data did not show any agreement with the proposed acid chloride structure. Herein, we described our opinion, which was supported by IR data, circumstantial evidences, and mechanistic insight, that the products obtained in the topical paper would be thioesters.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126352802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this note we discuss the Gauss-Lucas theorem (for the zeros of the derivative of a polynomial) and Speiser’s equivalent for the Riemann hypothesis (about the location of zeros of the Riemann zeta-function). We indicate similarities between these results and present there analogues in the context of elliptic curves, regular graphs, and finite Euler products.
{"title":"Speiser’s Theorem on the Road","authors":"Janyarak Tongsomporn, J. Steuding","doi":"10.48048/wjst.2019.6944","DOIUrl":"https://doi.org/10.48048/wjst.2019.6944","url":null,"abstract":"In this note we discuss the Gauss-Lucas theorem (for the zeros of the derivative of a polynomial) and Speiser’s equivalent for the Riemann hypothesis (about the location of zeros of the Riemann zeta-function). We indicate similarities between these results and present there analogues in the context of elliptic curves, regular graphs, and finite Euler products.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"326 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124599547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nidarat Choowichian, D. Thapinta, H. Sethabouppha, P. Thungjaroenkul
Stress is a significant health problem among nurses working in areas of political unrest and war. It can pose a negative impact on local health systems. This study aimed to explore the relationship of factors and their ability to predict stress. Factors included the severity of the situation of unrest, sense of coherence, commitment, self-efficacy, and social support that exists among nurses in these situations. The subjects were 300 nurses selected by multi-stage random sampling. Data were analyzed using Pearson’s product-moment correlation, and stepwise multiple regression. The results indicated that the severity of the situation of unrest was positively significantly associated with stress among nurses at a high level (r = .527, p < .01) and sense of coherence (r = - .272, p < .01) was negatively significantly associated with stress among nurses at a low level.The severity of the situation of unrest, sense of coherence, and social support together predicted 32.2 % of the variance in stress among nurses. The severity of the situation of unrest was the most significant predictor of stress (27.7 %), followed by sense of coherence (3.4 %) and social support (2.1 %). Two factors associated with stress were the severity of the situation of unrest and sense of coherence, and three factors that predicted stress among nurses were the severity of the situation of unrest, sense of coherence, and social support. The findings can be used as basic data for nursing administrators to plan actions to prevent and deal with stress among nurses in situations of unrest by focusing on such predicting factors.
压力是在政治动荡和战争地区工作的护士的一个重要健康问题。它可能对当地卫生系统造成负面影响。本研究旨在探讨各因素之间的关系及其预测压力的能力。因素包括动荡局势的严重程度、连贯性、承诺、自我效能感和护士在这些情况下存在的社会支持。研究对象为采用多阶段随机抽样方法抽取的300名护士。数据分析采用Pearson积差相关和逐步多元回归。结果显示，不安情绪严重程度与高水平护士的压力呈显著正相关(r = 0.527, p < 0.01)，连贯性感与低水平护士的压力呈显著负相关(r = - 0.272, p < 0.01)。动荡局势的严重程度、连贯性和社会支持共同预测护士压力差异的32.2%。动荡局势的严重程度是最显著的压力预测因子(27.7%)，其次是一致性感(3.4%)和社会支持(2.1%)。与压力相关的两个因素是不安状况的严重程度和连贯感，预测护士压力的三个因素是不安状况的严重程度、连贯感和社会支持。研究结果可作为护理管理者在不稳定情况下预防和处理护士压力的基础数据，重点关注这些预测因素。
{"title":"Factors Predicting Stress among Nurses in the Situation of Unrest of the Four Southern Border Provinces of Thailand","authors":"Nidarat Choowichian, D. Thapinta, H. Sethabouppha, P. Thungjaroenkul","doi":"10.48048/wjst.2020.5870","DOIUrl":"https://doi.org/10.48048/wjst.2020.5870","url":null,"abstract":"Stress is a significant health problem among nurses working in areas of political unrest and war. It can pose a negative impact on local health systems. This study aimed to explore the relationship of factors and their ability to predict stress. Factors included the severity of the situation of unrest, sense of coherence, commitment, self-efficacy, and social support that exists among nurses in these situations. The subjects were 300 nurses selected by multi-stage random sampling. Data were analyzed using Pearson’s product-moment correlation, and stepwise multiple regression. The results indicated that the severity of the situation of unrest was positively significantly associated with stress among nurses at a high level (r = .527, p < .01) and sense of coherence (r = - .272, p < .01) was negatively significantly associated with stress among nurses at a low level.The severity of the situation of unrest, sense of coherence, and social support together predicted 32.2 % of the variance in stress among nurses. The severity of the situation of unrest was the most significant predictor of stress (27.7 %), followed by sense of coherence (3.4 %) and social support (2.1 %). Two factors associated with stress were the severity of the situation of unrest and sense of coherence, and three factors that predicted stress among nurses were the severity of the situation of unrest, sense of coherence, and social support. The findings can be used as basic data for nursing administrators to plan actions to prevent and deal with stress among nurses in situations of unrest by focusing on such predicting factors.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125464921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In spite of the fact that epiphytic algae are considered an important component of freshwater ecosystems, our knowledge of their diversity and distribution is still rather poor. Taxonomic study on the composition of epiphytic algae living on submerged leaf and root tissues of macrophyte Nymphaea pubescens Willd, found at Laguna de Bay (Philippines), was conducted. In total, 21 algal taxa were identified: 10 Cyanophyceae, 6 Trebouxiophyceae, and 5 Bacillariophyceae. The taxa described in this study represent 13 orders, 16 families, 18 genera, and 21 species based on the recent combined taxonomical approach. Of these taxa, the occurrence of a rare cyanobacteria, Chroococcus schizodermaticus West, is reported for the first time in the Philippines. One species is also reported here for the first time in the Philippines, based on current taxonomic nomenclature, and this is Cyanothece aeruginosa (Nägeli) Komárek, which is based on the former name of Synechococcus aeruginosus Nägeli. These taxonomic records are considered important basal information in enriching the knowledge about the diversity and habitat distribution of cyanobacteria and microalgae in macrophytes found in freshwater habitats in the Philippines.
尽管附生藻类被认为是淡水生态系统的重要组成部分，但我们对其多样性和分布的认识仍然相当贫乏。对菲律宾拉古纳德湾(Laguna de Bay)大型植物Nymphaea pubescens wild的沉叶和根组织中附生藻类的组成进行了分类研究。共鉴定出21个藻类类群:10个蓝藻门，6个海藻门，5个硅藻门。根据最新的综合分类方法，本研究的分类群包括13目16科18属21种。在这些分类群中，菲律宾首次报道了一种罕见的蓝藻，西裂皮蓝球菌。根据目前的分类学命名法，在菲律宾也首次报道了一种，这是Cyanothece aeruginosa (Nägeli) Komárek，它是根据铜绿聚球菌Nägeli的前身命名的。这些分类记录被认为是丰富菲律宾淡水生境中大型植物蓝藻和微藻多样性和生境分布的重要基础信息。
{"title":"Species Composition of Algal Epiphyton of Pink Lotus (Nymphaea pubescens Willd) Found in Laguna de Bay (Philippines)","authors":"E. Arguelles","doi":"10.48048/wjst.2020.5015","DOIUrl":"https://doi.org/10.48048/wjst.2020.5015","url":null,"abstract":"In spite of the fact that epiphytic algae are considered an important component of freshwater ecosystems, our knowledge of their diversity and distribution is still rather poor. Taxonomic study on the composition of epiphytic algae living on submerged leaf and root tissues of macrophyte Nymphaea pubescens Willd, found at Laguna de Bay (Philippines), was conducted. In total, 21 algal taxa were identified: 10 Cyanophyceae, 6 Trebouxiophyceae, and 5 Bacillariophyceae. The taxa described in this study represent 13 orders, 16 families, 18 genera, and 21 species based on the recent combined taxonomical approach. Of these taxa, the occurrence of a rare cyanobacteria, Chroococcus schizodermaticus West, is reported for the first time in the Philippines. One species is also reported here for the first time in the Philippines, based on current taxonomic nomenclature, and this is Cyanothece aeruginosa (Nägeli) Komárek, which is based on the former name of Synechococcus aeruginosus Nägeli. These taxonomic records are considered important basal information in enriching the knowledge about the diversity and habitat distribution of cyanobacteria and microalgae in macrophytes found in freshwater habitats in the Philippines.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126439988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nanasombat, Kanokwan Yansodthee, Ilada Jongjaited
Antidiabetic, antioxidant, anti-acetylcholinesterase and prebiotic activities, total phenolics and flavonoids of 33 crude ethanolic extracts of Thai local fruits, vegetables and some local food plants were determined. Mangosteen (Garcinia mangostana) fruit peel and Indian gooseberry (Phyllanthus emblica) fruit extracts had highest antioxidant activity. Bamboo grass (Tiliacora triandra) leaf extract had strongest α-amylase inhibitory activity (78.28 % inhibition), while mulberry (Morus alba) fruit extract had strongest α-glucosidase inhibitory activity (59.63 % inhibition). Star cactus (Aloe vera) leaf pulp extract had strongest anti-acetylcholinesteraseVactivity (31.55 % inhibition). Indian gooseberry fruit and mangosteen fruit peel extracts had highest content of total phenolics and flavonoids, respectively. The extract with highest indigestible polysaccharide content was the extract of mangosteen fruit peels (188.62 mg/g extract), while those with relatively high indigestible polysaccharides were the extracts of pineapple fruits (Ananas comosus), lotus seeds (Nelumbo nucifera), black rice grains (Oryza sativa) and pisang mas banana fruits (Musa sapientum). Based on these properties, 5 plant extracts were selected to study for their prebiotic effect on growth and fermentation of Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus in yogurt at 42 °C. Addition of lotus seed extract resulted in highest proliferation of these bacteria with 2.24 logCFU/g increase of total lactic acid bacterial counts in yogurt after 24 h fermentation, while addition of black rice grain, pisang mas banana fruit and pineapple fruit extracts caused good growth of these bacteria with 2.12 - 2.19 logCFU/g increase of total counts.
{"title":"Evaluation of Antidiabetic, Antioxidant and Other Phytochemical Properties of Thai Fruits, Vegetables and Some Local Food Plants","authors":"S. Nanasombat, Kanokwan Yansodthee, Ilada Jongjaited","doi":"10.48048/wjst.2019.3731","DOIUrl":"https://doi.org/10.48048/wjst.2019.3731","url":null,"abstract":"Antidiabetic, antioxidant, anti-acetylcholinesterase and prebiotic activities, total phenolics and flavonoids of 33 crude ethanolic extracts of Thai local fruits, vegetables and some local food plants were determined. Mangosteen (Garcinia mangostana) fruit peel and Indian gooseberry (Phyllanthus emblica) fruit extracts had highest antioxidant activity. Bamboo grass (Tiliacora triandra) leaf extract had strongest α-amylase inhibitory activity (78.28 % inhibition), while mulberry (Morus alba) fruit extract had strongest α-glucosidase inhibitory activity (59.63 % inhibition). Star cactus (Aloe vera) leaf pulp extract had strongest anti-acetylcholinesteraseVactivity (31.55 % inhibition). Indian gooseberry fruit and mangosteen fruit peel extracts had highest content of total phenolics and flavonoids, respectively. The extract with highest indigestible polysaccharide content was the extract of mangosteen fruit peels (188.62 mg/g extract), while those with relatively high indigestible polysaccharides were the extracts of pineapple fruits (Ananas comosus), lotus seeds (Nelumbo nucifera), black rice grains (Oryza sativa) and pisang mas banana fruits (Musa sapientum). Based on these properties, 5 plant extracts were selected to study for their prebiotic effect on growth and fermentation of Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus in yogurt at 42 °C. Addition of lotus seed extract resulted in highest proliferation of these bacteria with 2.24 logCFU/g increase of total lactic acid bacterial counts in yogurt after 24 h fermentation, while addition of black rice grain, pisang mas banana fruit and pineapple fruit extracts caused good growth of these bacteria with 2.12 - 2.19 logCFU/g increase of total counts.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117336424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genomic DNA extracted from human saliva samples showed high inter-subject variations in DNA yield, compelling the need to explore a methodology for the accurate quantitation of the extracted genomic DNA. Quantitative assessment of DNA extracted from saliva was achieved using human coagulation factor XIII as an internal control for subsequent downstream applications of amplification of human leucocyte antigen (HLA) genes by PCR. The PCR signals for the HLA target genes, namely, HLA-A, -B, -C , DPB1, DQB1, and DRB1 of exons 2 and 3, improved greatly with the use of a combination of Taq DNA polymerase and AccuPrimeTaq DNA polymerase. We also describe a new method of using dried saliva spots (DSS) as an alternate source of genomic DNA for HLA typing. PCR-based typing of DNA from human saliva offers a potential method for HLA typing and amplification, and typing of DNA, thus presented, could be applied in forensic science to saliva samples recovered from crime scenes.
{"title":"Human Saliva and Dried Saliva Spots as Source of DNA for PCR based HLA Typing using a Combination of Taq DNA Polymerase and AccuPrimeTaq Polymerase","authors":"Prerana Madhusudhana Murthy, Anupama Cheleri Neduvat, Cheemalamarri Veenadhar, Sudarson Sundarrajan, S. Padmanabhan","doi":"10.48048/wjst.2020.4430","DOIUrl":"https://doi.org/10.48048/wjst.2020.4430","url":null,"abstract":"Genomic DNA extracted from human saliva samples showed high inter-subject variations in DNA yield, compelling the need to explore a methodology for the accurate quantitation of the extracted genomic DNA. Quantitative assessment of DNA extracted from saliva was achieved using human coagulation factor XIII as an internal control for subsequent downstream applications of amplification of human leucocyte antigen (HLA) genes by PCR. The PCR signals for the HLA target genes, namely, HLA-A, -B, -C , DPB1, DQB1, and DRB1 of exons 2 and 3, improved greatly with the use of a combination of Taq DNA polymerase and AccuPrimeTaq DNA polymerase. We also describe a new method of using dried saliva spots (DSS) as an alternate source of genomic DNA for HLA typing. PCR-based typing of DNA from human saliva offers a potential method for HLA typing and amplification, and typing of DNA, thus presented, could be applied in forensic science to saliva samples recovered from crime scenes.","PeriodicalId":255195,"journal":{"name":"Walailak Journal of Science and Technology (WJST)","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121607862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：