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A Study of the Extragalactic UV Radiation in Helix Nebula using GALEX 利用GALEX对螺旋星云河外紫外线辐射的研究
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.23982
Lakshmi S. Bose
We have studied the ultraviolet sources using Galaxy Evolution Explorer medium imaging surveys in Helix Nebula and estimated UV fluxes by using aperture photometry in distant and near ultraviolet bands. The aperture photometric method produces reliable, accurate flux measurements and found inconsistent with the merged catalog of Galaxy Evolution Explorer. From the current results, the fluxes are consistent with brighter absolute magnitude up to 24.5 and the measurement error increases gradually to more than 50 % at the fainter magnitude side. Percentage of error in far UV is greater than near UV, due to the fact that brighter galaxies are more visible than the near UV sources. The diffuse UV contributors of zodiacal light, airglow contribution in the nebula were estimated. The total extragalactic UV radiation from the detected sources to the diffuse background in the nebula is of the order of 50 ± 14 photons cm-2sr-1s-1Å-1 in NUV band and 28 ±10 photons cm-2sr-1s-1Å-1 in FUV band. HIGHLIGHTS GALEX observations have the potential to find extragalactic UV sources Helix Nebula is first identified for distinct source detection Aperture photometric method can detect fainter sources up to the magnitude of 27 Extragalactic sources in the Helix nebula contribute to diffuse UV emission in the nebula GRAPHICAL ABSTRACT
我们利用星系演化探索者在螺旋星云的介质成像巡天研究了紫外源,并利用孔径光度法在远紫外和近紫外波段估计了紫外通量。孔径光度法产生可靠、准确的通量测量,但发现与星系演化探索者合并的目录不一致。从目前的结果来看,通量与较亮的绝对星等一致,达到24.5,在较暗的绝对星等处测量误差逐渐增加到50%以上。远紫外的误差百分比大于近紫外,因为明亮的星系比近紫外源更可见。估计了星云中黄道光的漫射紫外贡献量和气辉贡献量。从探测源到星云漫射背景的总河外紫外线辐射在NUV波段为50±14个光子cm-2sr-1s-1Å-1,在FUV波段为28±10个光子cm-2sr-1s-1Å-1。GALEX观测具有发现河外紫外线源的潜力螺旋星云首次被确定为不同的光源探测孔径光度法可以探测到27等的较弱的光源螺旋星云中的河外光源有助于星云中的漫射紫外线发射
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引用次数: 1
Implementation of 5.1 Sidra Intersection Software for Appraisal of Road Corridors under Current Form 5.1 Sidra交叉口软件在当前形式下道路廊道评价的实施
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.9703
Dagimwork Asele Manuka
The major goal of this study was to compute the flow appearances of the chosen midblock and to evaluate the road sections using various performance metrics that analyzed these road sections in both current and future conditions. Performance measure of flow parameters was at the operational period of the road. Therefore, this work examined the 2-way 2-lane roads with various performance measures. The capacity of mid blocks was also determined by plotting capacity curves and the level of service arrived and Sidra Intersection 5.1 tools were used for the analysis. All midblock evaluated with different performance measures both in current and future conditions with basic considerations. The analysis was done by adopting Sidra Intersection 5.1 tool and showed that 2-way 2-lane roads in future conditions were studied and the result indicated that their average travel speed, degree of saturation, practical spare capacity, total effective capacity, demand of flow, and level of service (LOS) displayed major changes from the base condition.HIGHLIGHTSCompute the flow appearances of the chosen midblockTo evaluate the road sections using various performance metrics that analyzed these road sections in both current and future conditionsPerformance measure of flow parameters was at the operational period of the road. Therefore, this work examined the 2-way 2-lane roads with various performance measuresThe capacity of mid blocks was also determined by plotting capacity curvesThe level of service arrived and Sidra Intersection 5.1 tools were used for the analysisGRAPHICAL ABSTRACT
本研究的主要目标是计算所选中间街区的流量外观,并使用各种性能指标来评估路段,这些指标分析了当前和未来条件下这些路段。流量参数的性能度量是在道路运行期间进行的。因此,本研究考察了具有不同性能指标的双向双车道道路。通过绘制容量曲线和到达的服务水平确定中间街区的容量,并使用Sidra Intersection 5.1工具进行分析。所有中间区块在当前和未来条件下都采用不同的性能指标进行评估,并考虑基本因素。采用Sidra Intersection 5.1工具进行分析,结果表明,研究了未来条件下的双向双车道道路,其平均行驶速度、饱和程度、实际备用容量、总有效容量、流量需求和服务水平(LOS)与基础条件相比变化较大。highlights计算所选中间街区的流量外观使用各种性能指标来评估路段,这些指标分析了这些路段在当前和未来的条件下的流量参数的性能度量是在道路的运行期间。因此,本研究考察了具有各种性能指标的双向双车道道路,通过绘制容量曲线确定了中间街区的容量,到达的服务水平和Sidra十字路口5.1工具用于分析
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引用次数: 0
Experimental Investigation of Process Parameters of Al-SiC-B4C MMCs Finished by a Novel Magnetic Abrasive Flow Machining Setup 新型磁磨料流加工Al-SiC-B4C复合材料工艺参数的实验研究
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.9885
Gagandeep Chawla, V. Mittal, Sushil Mittal
Abrasive flow machining (AFM) is one of the non-conventional finishing processes used to attain good surface quality and high material removal. However, limited attempts have been made to improve the performance of these processes. This paper presents a novel magnetic abrasive flow machining (MAFM) setup fabricated by adding a magnetization effect in which a nylon fixture and permanent magnets are replaced by a newly fabricated aluminium fixture and coil-type magnets, respectively. Inner cylindrical surfaces of hybrid Al/SiC/B4C metal matrix composites (MMCs) are finished by the MAFM process. One variable at a time (OVAT) approach is used for studying the effect of 6 input parameters, extrusion pressure (Ep), the number of cycles (N), abrasives concentration (C), workpiece material (Wp), abrasive mesh size (M), and magnetic flux density (Mf) upon response parameters, material removal rate (MRR) and change in surface roughness (ΔRa). The experimental results obtained for MRR and ΔRa show a significant improvement from 3.92 to 7.68 μg/s and 0.49 to 0.74 μm, respectively due to the increase of the extrusion pressure from 1 to 9 Mpa. The MRR and ΔRa was reduced from 6.89 to 6.78 μg/s and 0.46 to 0.22 μm, respectively with an increase in mesh number of abrasives from 80 to 400. The variation in concentration of abrasives from 40 to 60 % shows an improvement in MRR from 4.51 to 6.42 μg/s; whereas, there is a negligible effect on ΔRa which comes out from 3.82 to 3.86 μm. The MMCs, which are used for the experimentation shows a decline in MRR and ΔRa from 5.12 to 3.85 μg/s and 0.77 to 0.42 μm, respectively. This happened because there was a percentage change of reinforcement of SiC from 9 to 7 % and B4C from 1 to 3 % in Al-6063. An increase in the number of cycles from 50 to 250 shows a significant improvement in both MRR and ΔRa from 1.79 to 3.75 μg/s and 0.97 to 1.86 μm, respectively. Variation in magnetic effect also significantly improves MRR and ΔRa from 1.35 to 3.17 μg/s and 0.38 to 1.06 μm, respectively, when it is varied from 0.15 - 0.45 Tesla. The work carried out shows an overall significant improvement in MRR and ΔRa by using the MAFM process. The MAFM process finds a wide range of applications in finishing like surgical instruments, mechanical components, aerospace industry, electronics industry, etc.HIGHLIGHTSThe hybrid MMCs (Al/SiC/B4C) are finished by novel MAFM setupAn aluminium fixture and coil-type magnets play a significant role for finishing the workpiece surfacesAn abrasive laden media acts as a cutting tool in the finishing processThe OVAT approach is used for investigating the parametric effectThe extrusion pressure, number of cycles and magnetic flux density are the significant parameters affecting the MRR and ΔRaGRAPHICAL ABSTRACT
磨料流加工(AFM)是一种用于获得良好表面质量和高材料去除率的非常规精加工工艺。然而,为改进这些过程的性能所做的尝试有限。本文提出了一种新型的磁性磨粒流加工装置,该装置通过增加磁化效应,将尼龙夹具和永磁体分别替换为新制造的铝夹具和线圈式磁体。采用MAFM工艺制备了Al/SiC/B4C杂化金属基复合材料(mmc)的内圆柱形表面。采用一次一变量(OVAT)方法,研究了挤出压力(Ep)、循环次数(N)、磨料浓度(C)、工件材料(Wp)、磨料网目尺寸(M)、磁通密度(Mf) 6个输入参数对响应参数、材料去除率(MRR)和表面粗糙度变化的影响(ΔRa)。实验结果表明,当挤压压力从1 Mpa增加到9 Mpa时,MRR和ΔRa分别从3.92提高到7.68 μg/s和0.49提高到0.74 μm。磨料目数从80目增加到400目,MRR和ΔRa分别从6.89降低到6.78 μg/s和0.46降低到0.22 μm。磨料浓度从40%增加到60%,MRR从4.51增加到6.42 μg/s;而在3.82 ~ 3.86 μm范围内,对ΔRa的影响可以忽略不计。实验用MMCs的MRR和ΔRa分别从5.12下降到3.85 μg/s和0.77下降到0.42 μm。这是因为Al-6063中SiC的增强率从9%增加到7%,B4C的增强率从1%增加到3%。当循环次数从50次增加到250次时,MRR和ΔRa分别从1.79提高到3.75 μg/s和0.97提高到1.86 μm。在0.15 ~ 0.45特斯拉范围内,磁效应的变化也显著提高了磁阻比(MRR)和ΔRa,分别在1.35 ~ 3.17 μ s和0.38 ~ 1.06 μ s范围内。所开展的工作表明,通过使用MAFM工艺,MRR和ΔRa的总体显着改善。MAFM工艺在手术器械、机械部件、航空航天工业、电子工业等精加工中有着广泛的应用。亮点混合mmc (Al/SiC/B4C)由新型MAFM装置完成,铝制夹具和线圈式磁体在精加工工件表面方面起着重要作用,磨料负载介质在精加工过程中充当切削工具,OVAT方法用于研究参数效应。循环次数和磁通密度是影响MRR和ΔRaGRAPHICAL的重要参数
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引用次数: 5
A Single Mutation in the Carbohydrate-Binding Module Enhances Cellulase Activity in Bacillus Amyloliquefaciens Mutant 在解淀粉芽孢杆菌突变体中,碳水化合物结合模块的单一突变增强了纤维素酶的活性
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.23985
N. Polsa, C. Songsiriritthigul, Wasana Suyotha, Sugunya Suebsan, S. Anuntalabhochai, K. Sangwijit
From our earlier work, we modified the carbohydrate-binding module (CBM) of Bacillus amyloliquefaciens to increase cellulase activity using cold plasma technology. The cellulase gene (BglC-M) from the mutant was expressed in Escherichia coli BL21(DE3) under the T7 promoter. The hydrolysis activity of the cellulase mutant (BglC-M) was approximately 2.5-fold higher than the control (BglC-W) over a wide range of pH and temperature conditions. The amino acid sequence of the mutant BglC-M contained 471 residues that were almost identical to the control BglC-W. Only a single amino acid, lysine, was replaced by glutamic acid at position 370 (K370E) within the carbohydrate-binding module (CBM). Structure prediction and substrate docking of BglC-M indicated that the single mutation (K370E) might involve cellulose binding of the β-sandwich facilitated by hydrogen bonding. The docking study of cellopentaose with the model structure of BglC-M indicated that the replacement of lysine-370 led to the formation of a hydrogen bond with 436Y, which has a shorter distance (2.6 Å) compared with the control (5.4 Å). As a result, the structure becomes more compact and stable, resulting in increased catalytic efficiency. Finally, the biomass hydrolysis ability of cellulase was investigated on lignocellulosic wastes such as pineapple peel, corncob, and durian peel. The BglC-M enzyme showed a more significant amount of reducing sugar released from all lignocellulosic wastes than the control. This was the first evidence that altering the base composition of the cellulose binding module enhanced the catalytic activity. HIGHLIGHTS Increasing cellulase activity of Bacillus amyloliquefaciens using plasma technology Mutation at cellulose-binding module enhance cellulase hydrolysis activity Greater cellulase activity in the hydrolysis on lignocellulosic wastes GRAPHICAL ABSTRACT
从我们早期的工作中,我们利用冷等离子体技术修改了解淀粉芽孢杆菌的碳水化合物结合模块(CBM),以提高纤维素酶的活性。该突变体的纤维素酶基因(BglC-M)在大肠杆菌BL21(DE3)中T7启动子下表达。在较宽的pH和温度范围内,纤维素酶突变体(BglC-M)的水解活性比对照(BglC-W)高约2.5倍。突变体BglC-M的氨基酸序列包含471个残基,与对照BglC-W几乎相同。在碳水化合物结合模块(CBM)中,只有一个氨基酸赖氨酸被谷氨酸取代,位置为370 (K370E)。BglC-M的结构预测和底物对接表明,单突变(K370E)可能与纤维素通过氢键促进β-三明治的结合有关。纤维素戊糖酶与BglC-M模型结构的对接研究表明,赖氨酸-370的取代导致与436Y形成氢键,与对照(5.4 Å)相比,氢键的距离更短(2.6 Å)。因此,结构变得更加紧凑和稳定,从而提高了催化效率。最后,研究了纤维素酶对菠萝皮、玉米芯、榴莲皮等木质纤维素废弃物的水解能力。与对照相比,BglC-M酶从所有木质纤维素废物中释放的还原糖量更大。这是改变纤维素结合模块的碱基组成增强催化活性的第一个证据。利用等离子体技术提高解淀粉芽孢杆菌的纤维素酶活性纤维素结合模块的突变提高了纤维素酶的水解活性纤维素酶在木质纤维素废物水解中的活性更高
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引用次数: 0
Soil Chemical Properties and Maize (Zea mays L.) Yield influenced by Lime and Fern (Pteris vittata) 土壤化学性质与玉米(Zea mays L.)石灰和蕨类植物对产量的影响
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.10025
N. Chuong, T. Bush
This study aimed to investigate the influence of liming and fern on reducing the absorption of arsenic (As) by maize and As content in the soil. The single-factor experiment was designed in a completely random block (4 treatments and 4 replicates). Treatments were followed: Treatment 1 (NT1) liming (3tons CaO.ha-1); NT2: Plant ferns alternately with maize (without liming); NT3: Plant ferns alternately with maize and liming (3 tons CaO.ha-1); NT4: Control (no liming or ferns). The results showed that the applications of liming and ferns have positive influences on the soil pH, EC, OM, yield, and yield components of the maize tested in this study. The yield difference between the application of liming and intercropped ferns was increased from 5.4 to 22.3 %. Moreover, the arsenic contents in soil, stems, and seeds were 25.7, 32.0 and 50 % lower than that of the control, respectively. soil to roots and stems, which significantly caused reduction of a large amount of As content in soils. Therefore, to reduce the production cost, and enhance soil and maize quality, application of lime (3 ton.ha-1) and intercropped ferns is recommended.HIGHLIGHTSIncreasing pH, EC and organic matter by the lime application combined with fernsDecreasing the soil arsenic concentration by intercroping maizes and fernsThe lime application combined with ferns raising the yield components and yield of maizeThe high As accumulation of stems and shoots of ferns intercroping maizes and fernsThe lowest As accumulation of stems and seeds of maizes applying the lime combined with ferns
本研究旨在探讨石灰和蕨类植物对降低玉米对砷的吸收和土壤中砷含量的影响。单因素试验设计为完全随机区组(4个处理,4个重复)。处理1 (NT1)石灰(3吨草酸-1);NT2:蕨类植物与玉米交替种植(不施石灰);NT3:蕨类植物与玉米、石灰交替种植(3吨草河-1);NT4:控制(没有石灰或蕨类植物)。结果表明,施用石灰和蕨类植物对试验玉米土壤pH、EC、OM、产量和产量组成均有积极影响。施石灰与间作蕨类作物的产量差由5.4%提高到22.3%。土壤、茎和种子中砷含量分别比对照降低25.7%、32.0%和50%。使土壤中大量砷含量显著降低。因此,为降低生产成本,提高土壤和玉米品质,建议施用石灰(3吨/公顷)和间作蕨类植物。短句来源石灰与蕨类混作提高了土壤的pH、EC和有机质降低了玉米与蕨类混作降低了土壤的砷浓度,提高了玉米的产量构成和产量,蕨类混作的茎、梢砷积累量高,石灰与蕨类混作的茎、梢砷积累量低
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引用次数: 1
Application of the Simple Verification Method to Estimate the Weather at Makassar Maritime Station, Indonesia 简单验证方法在印尼望加锡海事站天气预报中的应用
Pub Date : 2021-09-13 DOI: 10.48048/wjst.2021.9542
D. Didiharyono, G. Giarno
Verification is used to measure the quality of a weather prediction, improve process performance, and measure the value of weather estimation. Initially, weather verification developed after Finley published his paper on the verification of tornado events. The type of data, objectives, and scale can make a different method in using weather verification. If there are some parameters that can be predicted, a simple question is consequently often asked by the public: how accurate are weather forecasts? Nowadays, the public wants a simple answer in 1 value that is presented quantitatively. The aim of the research is to develop a simple method that can answer the accuracy of weather prediction in a value that is easily understood by the public. Practically, validation comparing between prediction and observation parameters is divided into 2, namely dichotomous and comparing the values. This research tries to combine all weather prediction variables into a dichotomous variable with a threshold. Moreover, this technique is tested on weather predictions for the port of Makassar over a year. The results show that a certain threshold can be used to change the weather variable to be dichotomous. With the application of this method, forecast accuracy and suitability between the predicted parameters can be obtained. Moreover, the weather forecast issued by the Makassar Maritime Station shows the average true value of the forecast to be 69.1 %, and then the capabilities vary by forecasters, which range from 61 to 79 %.HIGHLIGHTSWeather forecast verification is used to measure the quality of a weather forecast, improve process performance, and the value of weather forecastsThe character of the weather variables and their predictions is unique and influences the type of evaluation methodTo facilitate the public's assessment of the accuracy of weather predictions, it is necessary to combine weather prediction evaluation methods in one valueUsing the tolerance threshold whether a deviant prediction is used to combine various weather predictive variablesAverage true value of the forecast is 69.1 % and the different capabilities of each forecaster, which range from 61 to 79 %GRAPHICAL ABSTRACT
验证用于测量天气预报的质量,改进过程性能,并测量天气估计的价值。最初,天气验证是在Finley发表了关于龙卷风事件验证的论文之后发展起来的。数据类型、目标和规模可以使使用天气验证的方法不同。如果有一些参数是可以预测的,那么公众经常会问一个简单的问题:天气预报有多准确?如今,公众想要一个简单的答案,一个量化的价值。这项研究的目的是开发一种简单的方法,可以回答天气预报的准确性,以一个容易被公众理解的值。实际中,预测参数与观测参数的验证比较分为两种,即二分类和值比较。本研究试图将所有天气预报变量组合成一个带有阈值的二分类变量。此外,这项技术在望加锡港一年多的天气预报中进行了测试。结果表明,采用一定的阈值可以使天气变量变为二分类。应用该方法,可以获得预测参数之间的预测精度和适用性。此外,望加锡海事站发布的天气预报显示,预报的平均真实值为69.1%,然后各预报员的能力各不相同,在61%至79%之间。highlight天气预报验证是用来衡量天气预报的质素,改善过程的表现,以及天气预报的价值。天气变量及其预测的特点是独特的,并会影响评估方法的类型。利用容差阈值对不同天气预报变量是否使用偏差预报进行组合,预测的平均真值为69.1%,而每个预报员的能力不同,其范围从61%到79%不等
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引用次数: 4
A Low Noise High Input Impedance Chopper-Stabilized Biopotential Amplifier with Ripple Reduction Technique 采用纹波抑制技术的低噪声高输入阻抗斩波稳定生物电位放大器
Pub Date : 2021-09-11 DOI: 10.48048/wjst.2021.23974
Ankit Adesara, A. Naik
Biopotential signals are created as a result of the electrochemical activity of the many cells that comprise the nervous system, and they represent both normal and pathological organ function. These signals must be identified with extreme caution because they are surrounded by a great deal of noise when detected by sensors. This article explores a novel biopotential amplifier that incorporates the chopper stabilization technique to increase noise performance and minimize offset. However, by introducing the chopper modulator into the proposed design, the amplifier's overall input impedance was lowered, which was then increased to greater than 200 MΩ by adding the forward auxiliary path to the input branch. Additionally, the output ripple, produced due to switching activity and up-sampling, was reduced by inclusion of the R-C ripple removing block at the output of the operational transconductance amplifier (OTA). The designed architecture had a mid-band gain of 40dB with a power consumption of 9 µW and an offset of 10µV and a CMRR of 82 dB. It generated a noise of 42nV/√Hz. Also, the obtained results were compared with a conventional amplifier. The proposed design was verified by carrying out simulations using 180nm technology parameters. Cadence Virtuoso (Schematic editor), Spectre (Simulator), Symica and Magic (Layout) tools were used to complete the implementation and simulation of the proposed design. HIGHLIGHTS Biopotential signals are created as a result of the electrochemical activity of the many cells which must be identified with extreme caution because they are surrounded by a great deal of noise when detected by sensors It explores a novel biopotential amplifier that incorporates the chopper stabilization technique to increase noise performance and minimize offset By introducing the chopper modulator into the proposed design, the amplifier's overall input impedance was lowered, which was then increased to greater than 200 MΩ by adding the forward auxiliary path to the input branch The output ripple, produced due to switching activity and up-sampling, was reduced by inclusion of the R-C ripple removing block at the output of the operational transconductance amplifier (OTA) The designed architecture had a mid-band gain of 40dB with a power consumption of 9 µW and an offset of 10 µV and a CMRR of 82 dB. It generated a noise of 42 nV/√Hz GRAPHICAL ABSTRACT
生物电位信号是由组成神经系统的许多细胞的电化学活动产生的,它们代表了正常和病理器官的功能。这些信号必须非常小心地识别,因为当传感器检测到它们时,它们周围有大量的噪声。本文探讨了一种新型的生物电位放大器,该放大器采用斩波稳定技术来提高噪声性能并最小化偏移。然而,通过在所提出的设计中引入斩波调制器,放大器的整体输入阻抗降低,然后通过在输入支路中添加正向辅助路径将其增加到大于200 MΩ。此外,由于开关活动和上采样而产生的输出纹波,通过在操作跨导放大器(OTA)的输出端加入R-C纹波去除块而减少。所设计的结构中频段增益为40dB,功耗为9µW,偏移量为10µV, CMRR为82 dB。产生42nV/√Hz的噪声。并将所得结果与传统放大器进行了比较。采用180nm工艺参数进行了仿真验证。使用Cadence Virtuoso(原理图编辑器)、Spectre(模拟器)、Symica和Magic(布局)工具完成了所提出设计的实现和仿真。生物电位信号是由许多细胞的电化学活动产生的,必须非常小心地识别,因为当传感器检测到这些细胞时,它们被大量的噪声包围着。它探索了一种新型的生物电位放大器,该放大器采用斩波稳定技术来提高噪声性能并最小化偏置。通过在拟议的设计中引入斩波调制器,放大器的整体输入阻抗降低。在运维跨导放大器(OTA)的输出端加入R-C纹波去除块,减少了由于开关活动和上采样而产生的输出纹波。设计的结构具有40dB的中频增益,功耗为9 μ W,偏移量为10 μ V, CMRR为82 dB。产生42 nV/√Hz的噪声
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引用次数: 0
The Profile Analysis of Lactic Acid Bacteria (LAB) from Sumbawa White Honey and Its Potential Producing Antibacterial Compounds 松巴哇白蜂蜜乳酸菌(LAB)谱分析及其抗菌化合物生产潜力
Pub Date : 2021-07-31 DOI: 10.48048/wjst.2021.22204
B. Manguntungi, A. Z. Mustopa, Lita Meilina, M. Nurfatwa, L. R. Vanggy, Shasmita Irawan, M. S. Tamzil, Tegar Aprilian, Y. Yulianti, Arsyadila Sophia Fidduha, Intan Nurani Wersian
Honey acts as an antibacterial without side effects, and also contains antiseptic substances which function to inhibit bacterial growth. This study aimed to isolate the Lactic Acid Bacteria (LAB) in Sumbawa white honey and the bioactive compounds produced as pathogenic antibacteria. The 1st stage in this study was the isolation of lactic acid bacteria (LAB) in Sumbawa white honey, then continued with a grading test, morphological test, catalase test, methyl red test, and the last test, namely the antimicrobial test against 5 pathogenic bacteria (Salmonellatyhposa, Staphylococcus aureus, Escherichia coli, Enterobacter ludwigii, and Leclerciaadecarboxylata). Data analysis was performed using the Analysis of variance (ANOVA) test at a confidence level of 0.05 with SPSS 24. Based on the results of sequencing analysis, it was found that the 5 selected isolates were Enterococcus faecium species. The Enterococcus faecium species obtained from the sequencing results had different strains. The accession numbers of the 5 Enterococcus faecium were: Isolate-03 with a percentage of 97.29 % (accession number: KU324920.1), Isolate-07 has a percent identity of 97.36 % (accession number: MF108201.1), Isolate-09 of 97.73 % (accession number: CP041261.3), Isolate-20 with a percentage of 96.40 % (accession number: MN511819.1), and Isolate-24 with a percentage of 98.61 % (accession number: KM495938.1). These isolates can inhibit the growth of all tested pathogenic bacteria treated with 100 % LAB metabolites and were not significantly different (p > 0.05) compared to a positive control (Ampicillin). HIGHLIGHTS Antibacterial compound of Lactic Acid Bacteria (LAB) from Sumbawa white honey Lactic acid bacteria isolation, characterization, and biosprosprection against pathogens Identified LAB by 16s rRNA sequencing gives five strains of Enterococcus faecium All identified LAB metabolites can inhibit all pathogens by similar inhibition percentage with Ampicillin GRAPHICAL ABSTRACT
蜂蜜是一种没有副作用的抗菌物质,还含有抑制细菌生长的防腐物质。本研究旨在分离松巴哇白蜂蜜中的乳酸菌及其病原菌活性物质。本研究首先从松巴哇白蜂蜜中分离乳酸菌(LAB),然后进行分级试验、形态学试验、过氧化氢酶试验、甲基红试验,最后进行5种病原菌(沙门氏菌、金黄色葡萄球菌、大肠杆菌、路德维希肠杆菌、乳酸菌)的抑菌试验。数据分析采用方差分析(ANOVA)检验,采用SPSS 24,置信水平为0.05。经测序分析,5株分离株均为粪肠球菌。从测序结果中得到的粪肠球菌种类有不同的菌株。5株粪肠球菌分别为:分离株-03的鉴定率为97.29%(登记号:KU324920.1),分离株-07的鉴定率为97.36%(登记号:MF108201.1),分离株-09的鉴定率为97.73%(登记号:CP041261.3),分离株-20的鉴定率为96.40%(登记号:MN511819.1),分离株-24的鉴定率为98.61%(登记号:KM495938.1)。经100%乳酸菌代谢物处理后,这些分离菌均能抑制所有病原菌的生长,与阳性对照氨苄青霉素相比差异不显著(p > 0.05)。乳酸菌的分离、鉴定及对病原菌的生物活性研究通过16s rRNA测序鉴定出5株粪肠球菌,所鉴定的乳酸菌代谢产物均能抑制所有病原菌,其抑制率与氨苄西林相似
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引用次数: 2
On Fully Closed Stable Modules 关于全封闭稳定模块
Pub Date : 2021-07-30 DOI: 10.48048/wjst.2021.9534
S. Al-Aeashi, B. Davvaz
In this paper, we studied the notion of the fully closed stable module and identified some basic properties of this notion. We also investigated some concepts which are related to this module. In addition, the notion of CL-duo and fully closed stable modules were also studied.HIGHLIGHTSStudying the concept of fully closed stable modulesConnect two concepts with important algebraic propertiesGiving new results to related concepts such as duo module closed multiplication module and closed monomorphism coretractable module
本文研究了全闭稳定模的概念,并给出了该概念的一些基本性质。我们还研究了与该模块相关的一些概念。此外,还研究了CL-duo和全闭稳定模的概念。研究了全闭稳定模的概念,将两个具有重要代数性质的概念联系起来,对双模、闭乘法模、闭单态可缩模等相关概念给出了新的结果
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引用次数: 0
Mode of Action and Antibacterial Activity of Ethanolic Ant Plant Tuber Extract Inhibiting Growth of Staphylococcus aureus and Escherichia coli 植物块茎乙醇提取物抑制金黄色葡萄球菌和大肠杆菌生长的作用模式及抑菌活性研究
Pub Date : 2021-07-20 DOI: 10.48048/WJST.2021.9255
T. Srisawat, Narueparn Sukkasam, Jirawadee Uppala, Chananchita Nilake, Kanokrat Keawchai, Amita Chujan, Chuthapond Musimun, P. Chumkaew, P. Permpoonpattana
The present study aimed to investigate the activity of Hydnophytum formicarum Jack (Ant Plant) tuber extract against Staphylococcus aureus and Escherichia coli and the mode of action of the extract on bacterial responses. The antibacterial activity was determined using agar well diffusion method. Resazurin-based 96-well microdilution method was used to determine the Minimal Inhibitory Concentrations (MIC) of the extract. Mode of action on bacterial death and response patterns to the extract were assessed by flow cytometry using membrane integrity and granularity profiles. The results, based on serial dilution and zones of inhibition against the bacteria, showed that 2,000 µg/well was potent against S. aureus (16.33±1.53 mm) and E. coli (14.33±0.58 mm). For S. aureus, the MIC concentration was 8,000 µg/mL, while 4,000 µg/mL concentration exhibited activity against E. coli. Flow cytometric profiles confirmed loss of intracellular components, followed by death, for both bacteria. Bacterial responses to the extract had consistent dose- and time-dependent behavior. This is the first mode of action study on bacterial responses to H. formicarum tuber extract. The results suggest that the extract of H. formicarum tuber could serve as a source of an initial active ingredient for developing effective medicines to battle infections caused by pathogens.HIGHLIGHTSAnt plant tuber extract had a strong antibacterial activity against S. aureus and E. coliIntracellular component and bacterial membrane were the remarkable target for action of the extractDose- and Time-response model for bacterial response to the extract was achieved using Flow cytometry
本研究旨在探讨蚁根提取物对金黄色葡萄球菌和大肠杆菌的抑菌活性及其作用机制。采用琼脂孔扩散法测定其抑菌活性。采用瑞沙脲为基础的96孔微量稀释法测定提取物的最低抑菌浓度(MIC)。对细菌死亡的作用模式和对提取物的反应模式通过流式细胞术使用膜完整性和粒度剖面进行评估。结果表明,2000µg/孔对金黄色葡萄球菌(16.33±1.53 mm)和大肠杆菌(14.33±0.58 mm)具有较强的抑制作用。对金黄色葡萄球菌的MIC浓度为8000µg/mL,对大肠杆菌的MIC浓度为4000µg/mL。流式细胞分析证实了这两种细菌的细胞内成分丢失,随后死亡。细菌对提取物的反应具有一致的剂量和时间依赖性。这是第一次对细菌对蚁根提取物反应的作用模式进行研究。研究结果表明,福尔卡兰块茎的提取物可以作为一种初始活性成分的来源,用于开发对抗病原体引起的感染的有效药物。HIGHLIGHTSAnt植物块茎提取物对金黄色葡萄球菌和大肠杆菌具有较强的抗菌活性,细胞内成分和细菌膜是该提取物的重要作用靶点。利用流式细胞术建立了细菌对该提取物的剂量和时间反应模型
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引用次数: 0
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Walailak Journal of Science and Technology (WJST)
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