Biosensors and Bioelectronics最新文献_第7页

Selective detection of Staphylococcus aureus in food matrices using a chemiluminescent peroxidase-like DNA nanomachine 利用化学发光过氧化物酶样DNA纳米机器选择性检测食物基质中的金黄色葡萄球菌。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-14 DOI: 10.1016/j.bios.2026.118408

Pavel Filatov , Vladislav A. Reushev , Ekaterina Shchekutieva , Georgy Otinov , Lili Feng , Tigran Vartanyan , Daria Gorbenko , Mikhail V. Zyuzin

The development of rapid, sensitive, and specific methods for nucleic acid detection is critical for molecular diagnostics and biosafety. Here, we report the design and validation of a peroxidase-like DNA nanomachine (PxDm) that integrates target-templated assembly of a split G-quadruplex (G4) DNAzyme with chemiluminescent (CL) detection via photon counting in a compact, closed-cuvette device for the accurate detection of S. aureus. Following systematic optimization of the CL reaction, the PxDm platform demonstrated robust analytical performance at room temperature, reaching a nanomolar limit of detection (LOD) for single-stranded DNA (ssDNA) approximately 30 min after the start of the analysis. The assay was shown to be able of discriminating target sequences from those bearing single-nucleotide substitutions (SNS) with up to 98 % signal reduction. This CL approach proved to be more sensitive than colorimetric and fluorescent detection modalities for the same PxDm, with a 19.6-fold and 4.3-fold lower LOD, respectively. The platform's versatility was demonstrated through successful adaptation for double-stranded DNA (dsDNA) detection and integration with dual-priming isothermal amplification (DAMP). The integrated DAMP–PxDm assay achieved clinically relevant sensitivity, with a detection limit of 227 genomic equivalents, while maintaining high selectivity, as indicated by reduction factor (RF) values of 62.1 % for non-target DNA amplicons and 63.9 % for simulated target amplicons containing an SNS. The system demonstrated robustness in complex matrices, accurately detecting S. aureus amplicons from bacteria recovered from lettuce and spinach leaves, even in the presence of non-target organisms. The PxDm platform offers a powerful combination of low-nanomolar sensitivity, single-nucleotide specificity, and sufficient operational time, establishing it as a promising tool for on-site pathogen detection and environmental monitoring applications.

发展快速、灵敏、特异的核酸检测方法对分子诊断和生物安全至关重要。在这里，我们报道了一种类似过氧化物酶的DNA纳米机器（PxDm）的设计和验证，该机器将分裂g -四重体（G4） DNAzyme的靶模板组装与化学发光（CL）检测结合在一个紧凑的封闭试管装置中，通过光子计数来精确检测金黄色葡萄球菌。在对CL反应进行系统优化后，PxDm平台在室温下表现出强大的分析性能，在分析开始后约30分钟达到了单链DNA （ssDNA）的纳摩尔检测限（LOD）。结果表明，该方法能够区分携带单核苷酸取代（SNS）的靶序列，信号降低率高达98%。对于相同的PxDm， CL方法比比色法和荧光检测方法更敏感，LOD分别低19.6倍和4.3倍。通过成功适应双链DNA （dsDNA）检测和与双引物等温扩增（DAMP）的集成，证明了该平台的多功能性。集成的DAMP-PxDm检测达到了临床相关的灵敏度，检测限为227个基因组当量，同时保持了高选择性，非目标DNA扩增子的还原因子（RF）值为62.1%，含有SNS的模拟目标扩增子的还原因子（RF）值为63.9%。该系统在复杂基质中表现出鲁棒性，即使在非目标生物存在的情况下，也能准确地从莴苣和菠菜叶中回收的细菌中检测出金黄色葡萄球菌扩增子。PxDm平台提供了低纳摩尔灵敏度、单核苷酸特异性和充足的操作时间的强大组合，使其成为现场病原体检测和环境监测应用的有前途的工具。

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引用次数: 0

Cardiomyocyte mechanical contraction sensitivity-enhanced biosensing for precise drug evaluation 心肌细胞机械收缩敏感性增强生物传感用于精确药物评价。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-14 DOI: 10.1016/j.bios.2026.118405

Tao Liang , Zhekun Jia , Chengyun Wang , Jiaru Fang , Dongxin Xu , Hao Wang , Ling Zou , Zhen Wang , Ning Hu

Cardiovascular diseases is the leading cause of mortality worldwide, underscoring the critical importance of preclinical drug screening and cardiac safety in cardiovascular therapeutics to mitigate the immense health and economic burdens. In vitro cardiomyocyte models have been utilized to develop drug evaluation biosensing platforms, leveraging their rhythmic mechanical contractions. However, conventional label-based methodologies pose challenges for long-term monitoring due to the drug adverse effect and phototoxicity, and video-based strategies necessitate high-resolution imaging but is constrained by low frame rates, while label-free biosensing platforms often require costly and time-consuming microfabrication techniques. Here, we introduce a mechanical contraction biosensing platform that incorporates a multi-channel size-regulable impedance sensor (SRIS) with a custom-developed detection system. The fabrication process of SRIS is straightforward and efficient, providing substantial benefits in terms of economic and temporal costs. Further, the SRIS platform strategically regulates three distinct electrode sizes, and the optimal driving frequency of each sensor is systematically determined. Through long-term dynamic cellular viability and contraction assessment, the cardiomyocyte-based contraction model is refined and prepared for drug evaluation. Three typical cardiac drugs are applied to modulate cardiomyocyte contraction function: a receptor agonist, an ion channel blocker, and a myosin inhibitor. The findings suggest that the SRIS platform proficiently captures the effects of both electrode size and drug concentration on cardiomyocyte contraction properties, thereby demonstrating its precise pharmacological assessment. This facilely fabricated, cost-effective, and size-regulable cardiomyocyte contraction biosensing platform holds significant potential for applications in cardiology and pharmacology research.

心血管疾病是世界范围内死亡的主要原因，这强调了临床前药物筛选和心血管治疗中的心脏安全性对于减轻巨大的健康和经济负担的重要性。体外心肌细胞模型已被用于开发药物评估生物传感平台，利用其有节奏的机械收缩。然而，由于药物副作用和光毒性，传统的基于标签的方法对长期监测构成了挑战，基于视频的策略需要高分辨率成像，但受到低帧速率的限制，而无标签生物传感平台通常需要昂贵且耗时的微制造技术。在这里，我们介绍了一种机械收缩生物传感平台，该平台结合了多通道尺寸可调阻抗传感器（SRIS）和定制开发的检测系统。SRIS的制造过程简单高效，在经济和时间成本方面提供了可观的效益。此外，SRIS平台战略性地调节了三种不同的电极尺寸，并系统地确定了每个传感器的最佳驱动频率。通过长期动态细胞活力和收缩评估，完善基于心肌细胞的收缩模型，为药物评价做准备。三种典型的心脏药物用于调节心肌细胞收缩功能：受体激动剂、离子通道阻滞剂和肌球蛋白抑制剂。研究结果表明，SRIS平台可以熟练地捕捉电极大小和药物浓度对心肌细胞收缩特性的影响，从而证明其精确的药理学评估。这种易于制造，成本效益高，尺寸可调节的心肌细胞收缩生物传感平台在心脏病学和药理学研究中具有重要的应用潜力。

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引用次数: 0

Construction of a multilayer fluorescence-encoded single quantum dot-based FRET nanosensor for simple and sensitive monitoring of circular RNA in clinical tissues 多层荧光编码单量子点FRET纳米传感器的构建，用于临床组织中环状RNA的简单灵敏监测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-13 DOI: 10.1016/j.bios.2026.118401

Qingyi Wang , Jinping Hu , Jun Lu , Chun-yang Zhang

Circular RNAs (circRNAs) represent a new category of evolutionarily conserved covalent-closed RNAs with critical roles in tumorigenesis and progression of various cancers. The accurate and sensitive monitoring of circRNAs is essential for early disease diagnosis and therapeutic monitoring. Herein, we construct a multilayer fluorescence encoding-mediated single QD-based Förster resonance energy-transfer (FRET) nanosensor for the simple and sensitive monitoring of circRNA in breast tumor cells and clinical tissues. This nanosensor involves only a multi-functionalized hairpin switcher probe that integrates circRNA recognition, enzymatic amplification, and signal transduction. When target circRNA is absent, the hairpin switcher probe is tightly locked in an inactive state. The introduction of target circRNA to hybridize with the hairpin switcher probe will induce its conformational change through toehold-mediated strand migration, initiating the Klenow fragment polymerase-assisted sequential encoding of multiple Cy5 fluorophores into the stem of probes. Subsequently, these Cy5-labeled reaction products can be assembled onto 605QDs to form the QD-DNA-Cy5 nanostructures, producing remarkable FRET signals that can be directly counted through single-molecule imaging. Remarkably, this assay can be homogeneously and isothermally carried out without requiring any enrichment or separation steps, and the multilayer fluorescence encoding endows the system with great operation simplicity and high analytical performance. This nanosensor achieves a detection limit of 24.16 aM, and can be extended to monitor intracellular circMTO1 expression at single-cell level. Moreover, it can accurately distinguish breast cancer tumor from healthy counterparts, providing a new platform for clinical diagnosis.

环状rna （circRNAs）是一类新的进化保守的共价封闭rna，在肿瘤发生和各种癌症的进展中起着关键作用。准确灵敏地监测环状rna对于疾病的早期诊断和治疗监测至关重要。在此，我们构建了一个多层荧光编码介导的单量子点Förster共振能量转移（FRET）纳米传感器，用于简单灵敏地监测乳腺肿瘤细胞和临床组织中的circRNA。这种纳米传感器只涉及一个多功能的发夹开关探针，集成了环状rna识别、酶扩增和信号转导。当目标环状rna缺失时，发夹开关探针被紧紧锁定在非活动状态。引入目标circRNA与发夹开关探针杂交，将通过支点介导的链迁移诱导其构象变化，启动Klenow片段聚合酶辅助的多个Cy5荧光团序列编码到探针茎中。随后，这些cy5标记的反应产物可以组装到605qd上，形成QD-DNA-Cy5纳米结构，产生显著的FRET信号，可以通过单分子成像直接计数。值得注意的是，该分析可以均匀等温进行，不需要任何富集或分离步骤，多层荧光编码使系统操作简单，分析性能高。该纳米传感器的检测限为24.16 aM，可以扩展到单细胞水平上监测细胞内circMTO1的表达。此外，它可以准确区分乳腺癌肿瘤与健康肿瘤，为临床诊断提供新的平台。

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引用次数: 0

Nanoarchitectonics of bacteriophage-based click fluorescent biosensing platform for detection of Pseudomonas fluorescens 基于噬菌体的荧光点击生物传感检测平台的纳米结构。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-13 DOI: 10.1016/j.bios.2026.118406

Heng Zhou , Xiru Zhang , Junyu Pan , Xinna Xie , Bo Qu , Feng Zhao , Yujun Jiang , Xianlong Zhang

Pseudomonas fluorescens (P. fluorescens) is a typical psychrotrophic bacterium responsible for raw milk spoilage and potential human infections, highlighting the need for rapid and reliable detection. In this study, we developed a clickase-based fluorescent biosensing platform for the sensitive and specific detection of P. fluorescens. Herein, a novel copper(I)/Cys–RGD nanocatalyst (namely CCRN) with excellent CuAAC reaction catalytic activity was developed, and the prepared CCRN was functionalized with a P. fluorescens–targeting bacteriophage to form a specific recognition probe (CCRN@Phage). Remarkably, integrating the CCRN@Phage probe with ConA@Fe₃O₄ enabled magnetic enrichment and bioorthogonal catalytic signal amplification for sensitive and specific detection of P. fluorescens without external reducing agents. Specifically, P. fluorescens was first enriched using ConA@Fe₃O₄ and then captured by the CCRN@Phage probe to form a sandwich complex. Upon magnetic isolation, the complex catalyzed a copper(I)–catalyzed azide−alkyne cycloaddition (CuAAC) reaction between 3–azido–7–hydroxycoumarin (azide 1) and 3–butyn–1–ol (alkyne 2), generating a fluorescent triazole product. The CCRN catalyst exhibited excellent stability and catalytic activity, functioning efficiently without dissolution or external reducing agents. The developed method for detection of P. fluorescens had a limit of detection (LOD) of 1 CFU/mL in a linear response from 10² to 10⁷ CFU/mL. This strategy was successfully applied to food samples, showing a great potential for practical applications in food safety monitoring.

荧光假单胞菌（P. fluorescens）是一种典型的嗜冷细菌，可导致原料奶变质和潜在的人类感染，因此需要快速可靠的检测。在这项研究中，我们开发了一个基于点击酶的荧光生物传感平台，用于荧光假单胞菌的敏感和特异性检测。本文开发了一种具有优异CuAAC反应催化活性的新型铜(I)/Cys-RGD纳米催化剂（即CCRN），并将制备的CCRN用P. fluorescens-targeting噬菌体功能化，形成特异性识别探针（CCRN@Phage）。值得注意的是，将CCRN@Phage探针与ConA@Fe3O4相结合，使磁性富集和生物正交催化信号放大能够在不使用外部还原剂的情况下对荧光假单胞菌进行敏感和特异性检测。具体来说，荧光假单胞菌首先通过ConA@Fe3O4富集，然后通过CCRN@Phage探针捕获形成三明治复合体。磁分离后，该配合物在3-叠氮-7-羟基香豆素（叠氮1）和3-丁-1-醇（炔2）之间催化铜(I)催化叠氮-炔环加成（CuAAC）反应，生成荧光三唑产物。CCRN催化剂表现出优异的稳定性和催化活性，无需溶解或外部还原剂即可有效发挥作用。在102 ~ 107 CFU/mL的线性范围内，荧光假单胞菌的检测限为1 CFU/mL。该策略已成功应用于食品样品，在食品安全监测中具有很大的实际应用潜力。

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引用次数: 0

A microfluidic microspheres accumulation platform for direct visualization of urine albumin-to-creatinine ratio in patients with chronic kidney disease 用于慢性肾病患者尿白蛋白与肌酐比值直接可视化的微流控微球积累平台。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-13 DOI: 10.1016/j.bios.2026.118400

Chenyu Cui , Hogi Hartanto , Xinxue Yin , Jiaheng Li , Choi Wan Luk , Cheuk Chun Szeto , Ting-Hsuan Chen

The urine albumin-to-creatinine ratio (uACR) level is an essential biomarker for the early screening and monitoring of chronic kidney disease (CKD), particularly among patients with diabetic and hypertension. However, uACR requires quantitative assay, where the use of designated analyzers makes the assays difficult for large-scale screening. In this study, we developed a decentralized, instrument-free microfluidic microspheres accumulation platform (MMAP) that visualizes the uACR in patients with diabetic nephropathy. Albumin inhibits the binding between magnetic microspheres (MMSs) and polymer microspheres (PMSs) due to competitive immunoassay interactions, while creatinine is recognized by the anti-creatinine aptamer, which releases a DNA blocker strand to facilitate connections between MMSs and PMSs through DNA hybridization. The binding of MMSs and PMSs prevents PMSs from escaping magnetic attraction in the inlet, thereby reducing the free PMS accumulation length in the measurement zone of the microfluidic chip. The PMS accumulation length allows for the visual quantification of uACR with excellent selectivity, tolerance to varying pH levels, and high accuracy comparable to that of standard tests in the hospital. As a convenient, low-cost assessment tool that correctly classified all clinical samples in this pilot cohort to evaluate CKD stages from normal to end-stage, this device offers significant benefits for disease self-monitoring and community health services.

尿白蛋白与肌酐比值（uACR）水平是慢性肾脏疾病（CKD）早期筛查和监测的重要生物标志物，特别是在糖尿病和高血压患者中。然而，uACR需要定量分析，其中使用指定的分析仪使得分析难以进行大规模筛选。在这项研究中，我们开发了一种分散的、无仪器的微流控微球积累平台（MMAP），用于可视化糖尿病肾病患者的uACR。白蛋白通过竞争性免疫分析相互作用抑制磁性微球（MMSs）和聚合物微球（PMSs）之间的结合，而肌酐则被抗肌酐适配体识别，并释放DNA阻断剂链，通过DNA杂交促进磁性微球和聚合物微球之间的连接。mms与PMS的结合防止了PMS在入口逃离磁场的吸引，从而减少了PMS在微流控芯片测量区的自由积累长度。PMS累积长度允许对uACR进行视觉量化，具有出色的选择性，对不同pH水平的耐受性，以及与医院标准测试相当的高精度。作为一种方便、低成本的评估工具，该设备正确分类了该试点队列中所有临床样本，以评估从正常到终末期的CKD阶段，为疾病自我监测和社区卫生服务提供了显著的好处。

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引用次数: 0

A miniaturized electrochemical biosensor for real-time in vivo potassium monitoring in the inner ear 一种用于内耳实时体内钾监测的小型电化学生物传感器。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-12 DOI: 10.1016/j.bios.2026.118388

Faezeh Arshadi , Mahsa Esfandiari , Robert Gay , Ya Lang Enke , Nona Farbehi , Christopher Pastras , Mohsen Asadnia

The ability to continuously monitor key biomarkers is crucial for advancing personalized medicine and enabling early disease intervention. Potassium ions (K⁺) play a vital role in neural signaling, cardiac function, and sensory processing, particularly in the cochlea, where potassium imbalances are linked to disorders such as Ménière's disease. However, existing diagnostic methods cannot monitor K⁺ in the inner ear in real time, limiting insight into the ionic mechanisms that underlie auditory function and related disorders. This study presents a miniaturized potassium-selective biosensor, incorporating a stable reference electrode, for continuous and real-time monitoring of K⁺ dynamics in the inner ear. The sensor underwent extensive benchtop validation, demonstrating high sensitivity (⁓52.8 mV/dec), a broad linear range (10⁻⁵–10⁻¹ mol/L), a limit of detection of 10⁻⁵·¹⁶ mol/L, strong selectivity coefficients (−2.62 for Na⁺, −4.10 for Mg²⁺, and −3.85 for Ca²⁺), and excellent stability over two months. In-vivo experiments in a guinea pig model confirmed the biosensor's capability to track dynamic potassium fluctuations under physiological conditions, such as responses to controlled potassium administration. These findings establish the feasibility of real-time K⁺ monitoring in the cochlea, providing a valuable tool for studying auditory physiology, understanding potassium regulation in the inner ear, and advancing targeted diagnostics and treatments for inner ear disorders.

持续监测关键生物标志物的能力对于推进个性化医疗和实现早期疾病干预至关重要。钾离子（K+）在神经信号传导、心脏功能和感觉处理中起着至关重要的作用，特别是在耳蜗中，钾离子失衡与msamni病等疾病有关。然而，现有的诊断方法不能实时监测内耳中的K+，限制了对听觉功能和相关疾病背后的离子机制的了解。本研究提出了一种小型化的钾选择性生物传感器，包含一个稳定的参比电极，用于连续和实时监测内耳中的K+动态。该传感器经过了广泛的台式验证，具有高灵敏度（⁓52.8 mV/dec），宽线性范围（10-5-10-1 mol/L），检测限为10- 5.16 mol/L，强选择性系数（Na+ -2.62, Mg2+ -4.10, Ca2+ -3.85）和两个月以上的优异稳定性。在豚鼠模型中进行的体内实验证实了该生物传感器在生理条件下跟踪动态钾波动的能力，例如对受控钾给药的反应。本研究结果为耳蜗实时监测钾离子的可行性奠定了基础，为研究听觉生理、了解内耳钾离子调控、推进内耳疾病的针对性诊断和治疗提供了有价值的工具。

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引用次数: 0

Single-use microneedle biosensor for detecting clinically relevant glucose in interstitial fluid 用于检测间质液中临床相关葡萄糖的一次性微针生物传感器

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-12 DOI: 10.1016/j.bios.2026.118387

Khaled Mohammed Saifullah , Zahra Faraji Rad

Effective diabetes management is increasingly shifting toward minimally invasive technologies that enable frequent and reliable assessment of glucose levels in interstitial fluid (ISF), enabling more informed and patient-centered monitoring. However, current approaches to glucose detection rely heavily on invasive blood-based glucometers or complex wearable devices for continuous monitoring. This study introduces a complete biosensing system using a novel swellable biocompatible microneedle (MN) array combined with chemically modified screen-printed electrodes (SPEs). Optimization of ISF collection was achieved using a customized applicator with variable vibration, which resulted in ISF uptake of 6.55 ± 0.47 μL (0Hz) and 7.06 ± 0.44 μL (100 Hz) within 5 min of application. The Prussian Blue/chitosan-SWCNT/GO_x/Nafion-modified SPE shows excellent sensitivity of 12.26 μA mM⁻¹ cm⁻², a detection limit of 0.08 mM, and high selectivity against common ISF interferents. In vitro and ex vivo validation across clinically relevant glucose concentrations showed strong linearity (R² = 0.989 and 0.978, respectively), with recovery exceeding 70 % in vitro and 50 % ex vivo compared with a commercial glucometer. This minimally invasive MN SPE platform enables reliable glucose quantification from microliter ISF volumes and shows strong potential for future multi-biomarker, point-of-care monitoring.

有效的糖尿病管理正越来越多地转向微创技术，这种技术能够频繁、可靠地评估间质液（ISF）中的葡萄糖水平，从而实现更明智和以患者为中心的监测。然而，目前的血糖检测方法严重依赖于侵入性血糖仪或复杂的可穿戴设备进行连续监测。本研究介绍了一种完整的生物传感系统，该系统使用一种新型的可膨胀生物相容性微针（MN）阵列与化学修饰的丝网印刷电极（spe）相结合。采用自定义的可变振动施药器对ISF采集进行优化，施药5 min内ISF吸收量分别为6.55±0.47 μL （0Hz）和7.06±0.44 μL （100 Hz）。普鲁士蓝/壳聚糖- swcnt /GOx/ nafon修饰的SPE灵敏度为12.26 μA mM−1 cm−2，检出限为0.08 mM，对常见的ISF干扰具有较高的选择性。在体外和离体验证中，与临床相关的葡萄糖浓度具有很强的线性关系（R2分别= 0.989和0.978），与市售血糖仪相比，体外和离体回收率分别超过70%和50%。这种微创MN SPE平台可以从微升ISF体积中进行可靠的葡萄糖定量，并显示出未来多生物标志物，护理点监测的强大潜力。

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引用次数: 0

A mediator-free microbial electrochemical biosensor based on Escherichia coli nucleotide metabolism for rapid toxicity assessment of emerging contaminants 一种基于大肠杆菌核苷酸代谢的无介质微生物电化学生物传感器，用于新兴污染物的快速毒性评估

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-12 DOI: 10.1016/j.bios.2026.118390

Qi Feng, Jiaying Wang, Zileng Yu, Zhipeng Zhang, Huizi Zheng, Jiao Qu, Xiaolin Zhu

To address the growing environmental risks associated with emerging contaminants and limited rapid toxicity assessment tools, we developed a mediator-free microbial electrochemical biosensor using Escherichia coli nucleotide metabolism. This biosensor enables the direct detection of electroactive metabolites without exogenous mediators by utilizing the oxidation peak current as a sensitive toxicity indicator. An electrode was modified with a nanocomposite of carboxylated multi-walled carbon nanotubes and zinc oxide (MWCNT-COOH/ZnO), which significantly enhanced the electrochemical signals. Mechanistic studies revealed that the detection signal originated from the oxidation of guanine and xanthine. The biosensor was used to detect four bisphenol analogues and two per-/polyfluoroalkyl substances. The toxicity followed the order of bisphenol AF > bisphenol A > bisphenol F > bisphenol S, suggesting that bisphenol AF posed a higher toxicity risk than bisphenol A. The half-maximal inhibitory concentration (IC₅₀) for perfluorooctanesulfonic acid was 1011.13 μM, and its substitute, 6:2 fluorotelomer sulfonic acid, exhibited significantly lower toxicity at equivalent concentrations. The biosensor was further applied to actual water samples from the Yitong River, revealing generally low toxicity (inhibition rate <30 %) with higher inhibition rates observed at densely populated urban sites. This biosensor is characterized by simple operation, fast response, low cost, and independence from water turbidity or color, thereby offering an efficient and sensitive analytical platform for both toxicity assessment of emerging contaminants and safety evaluation of real water samples.

为了解决与新出现的污染物和有限的快速毒性评估工具相关的日益增长的环境风险，我们开发了一种利用大肠杆菌核苷酸代谢的无介质微生物电化学生物传感器。这种生物传感器利用氧化峰电流作为敏感的毒性指标，无需外源介质即可直接检测电活性代谢物。采用羧化多壁碳纳米管-氧化锌纳米复合材料（MWCNT-COOH/ZnO）修饰电极，显著增强了电极的电化学信号。机理研究表明，检测信号来源于鸟嘌呤和黄嘌呤的氧化。该生物传感器用于检测四种双酚类似物和两种全/多氟烷基物质。毒性顺序为双酚AF >；双酚A >；双酚F >；双酚S，表明双酚AF的毒性风险高于双酚A。对全氟辛烷磺酸的半最大抑制浓度（IC50）为1011.13 μM，其替代品6:2氟端聚体磺酸在同等浓度下毒性明显低于双酚A。生物传感器进一步应用于伊通河的实际水样，显示总体毒性较低（抑制率约30%），在人口密集的城市地区观察到较高的抑制率。该生物传感器具有操作简单、响应速度快、成本低、不受水浊度或颜色影响等特点，为新出现污染物的毒性评估和真实水样的安全性评估提供了一个高效、灵敏的分析平台。

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引用次数: 0

Machine-learning-optimized T3C2Tx/Bi2Se3 nanoflower-modified screen-printed electrodes for electrochemical detection of trace Pb2+ and Cd2+ in artificial sweat 机器学习优化的T3C2Tx/Bi2Se3纳米花修饰丝网印刷电极用于人工汗液中痕量Pb2+和Cd2+的电化学检测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-12 DOI: 10.1016/j.bios.2026.118389

Haozi Lu , Wei Zhou , Ye Li , Meiqing Yang , Jifei Yang , Can Hu , Yabo Ogunduyile , Travis Hu , Gang Yu , Huimin Li , Ming Zhang , Song Liu

In the field of non-invasive detection methods, sweat analysis has been increasingly capturing the interest of researchers due to its potential for clinical and health monitoring applications. While the detection of various substances in sweat has been extensively documented, the detection of heavy metal ions such as cadmium (Cd²⁺) and lead (Pb²⁺) is challenging due to their low concentrations, often falling below 100 ppb. Sweat testing also faces challenges such as variability in sweat production and evaporation rates, biological fouling, and sensor sensitivity decay, which significantly affect the accurate measurement of biomarker levels in sweat. Traditionally, machine learning in the biosensing domain is often employed for final data analysis and fitting, but its application in optimizing experimental conditions is less common. In this study, we developed a sensing platform based on Ti₃C₂T_x/Bi₂Se₃ nanoflower-modified screen-printed carbon electrodes (SPCE) for the detection of Cd²⁺ and Pb²⁺ in simulated sweat. The sensor demonstrates a broad detection range and relatively low limits of detection (LOD), with a linear detection range of 10–150 ppb and an LOD of 2.88 ppb for Cd²⁺, and a linear detection range of 5–150 ppb and an LOD of 3.45 ppb for Pb²⁺. In addition, an LSBoost–NGO machine-learning framework was employed to model the dependence of the stripping current on key experimental variables and to identify operating conditions that maximize analytical performance. These results demonstrate the potential of Ti₃C₂T_x/Bi₂Se₃/SPCEs combined with machine-learning-guided optimization for sweat-based monitoring of trace heavy metal ion.

在非侵入性检测方法领域，汗液分析因其在临床和健康监测方面的潜在应用而越来越引起研究人员的兴趣。虽然汗液中各种物质的检测已被广泛记录，但镉（Cd2+）和铅（Pb2+）等重金属离子的检测具有挑战性，因为它们的浓度很低，通常低于100 ppb。汗液测试还面临着汗液产生和蒸发速率的可变性、生物污垢和传感器灵敏度衰减等挑战，这些挑战会显著影响汗液中生物标志物水平的准确测量。传统上，生物传感领域的机器学习通常用于最终数据分析和拟合，但其在优化实验条件方面的应用较少。在这项研究中，我们开发了一个基于Ti3C2Tx/Bi2Se3纳米花修饰的丝网印刷碳电极（SPCE）的传感平台，用于检测模拟汗液中的Cd2+和Pb2+。该传感器具有较宽的检测范围和较低的检测限（LOD），对Cd2+的线性检测范围为10-150 ppb， LOD为2.88 ppb，对Pb2+的线性检测范围为5-150 ppb， LOD为3.45 ppb。此外，采用LSBoost-NGO机器学习框架来模拟剥离电流对关键实验变量的依赖性，并确定最大限度提高分析性能的操作条件。这些结果证明了Ti3C2Tx/Bi2Se3/ spce结合机器学习引导优化在基于汗液的痕量重金属离子监测中的潜力。

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引用次数: 0

A synthetic gene circuit to convert biochemical signals to electrical signal in engineered sensory bacteria for electrochemical biosensing 一种将生物化学信号转化为电信号的合成基因电路，用于电化学生物传感。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2026-01-12 DOI: 10.1016/j.bios.2026.118391

Xinlu Wu , Tianxiang Wei , Jingwen Zhang , Jinming Zhao , Kai Xu , Haijia Yu , Tao Gao

The intracellular gene circuit to transform biochemical signals to an electrical signal is the key for biosensor development to gap the information mismatch at the bio-electric interface, but a qualified gene circuit is difficult to design. In this study, we have shown the construction of an integrated synthetic gene circuit including the nitric oxide (NO)-responsive module and the phenylenediamine-1-carboxylic acid (PCA) synthesis module, to (1) equip a less renounced electroactive bacterium (e.g. Escherichia coli) with indirect electron transfer (IET) pathway to enhance electrical signal output, as well as (2) couple the IET gene circuit with the responsive gene circuit to sense small signal molecule, NO. The subsequent oxidation of PCA can be electrochemically quantified by the microelectrode, thereby establishing a signaling pathway from intracellular message to redox mediator, and finally to electrical signal output. In this way, the constructed microbial electrochemical biosensor for intracellular in situ NO analysis at the single-cell level owns high sensitivity, a wide linear detection range (100–2500 nM), and excellent selectivity. Therefore, this work shows an example for developing next-generation electroactive microorganisms (EAMs)-based electrochemical biosensors through synthetic biology tools with tailored and intelligent functionalities.

细胞内基因电路将生化信号转化为电信号是生物传感器发展的关键，以弥补生物电界面上的信息不匹配，但一个合格的基因电路很难设计。在本研究中，我们构建了一个集成的合成基因电路，包括一氧化氮（NO）响应模块和苯二胺-1-羧酸（PCA）合成模块，以(1)为电活性较弱的细菌（如大肠杆菌）提供间接电子转移（IET）途径以增强电信号输出，以及(2)将IET基因电路与响应基因电路偶联以感知小信号分子NO。PCA随后的氧化过程可以通过微电极进行电化学量化，从而建立了从细胞内信息到氧化还原介质，最后到电信号输出的信号通路。由此构建的微生物电化学生物传感器具有灵敏度高、线性检测范围宽（100 ~ 2500 nM）、选择性好等特点，可用于单细胞水平细胞内原位NO分析。因此，这项工作为通过具有定制和智能功能的合成生物学工具开发下一代基于电活性微生物（EAMs）的电化学生物传感器提供了一个例子。

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引用次数: 0