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Diallel Analysis of Genotype-Environment Interaction for Phenotypic Expressions of a Quantitative Character in Nicotiana rustica L. 烟叶一个数量性状表型表达的基因型-环境互作双列分析。
Pub Date : 1986-03-01 DOI: 10.1270/JSBBS1951.36.54
H. Miura, Y. Shimamoto, C. Tsuda
The intragenotypic varlability of a quantitative character can be measured in two ways : (1) variation of mean phenotype to different enviornments and known. as phenotypic plasticity. The latter is generally attributed to developmental instability which is assumed to be due to accidents in the developmental processes of individual plants and unrelated to external environmental conditions. The genetic analysis of the intragenotypic variability for plant height was carried out by mea.ns of complete diallel crosses of six inbred lines in rustica tobacco (Nicotiana rustica L.) under seven conditions. The magnitude of inter-plant variation of plant height was estimated from standard deviation within plot.
数量性状的显型内变异可以用两种方法来衡量:(1)平均表型对不同环境和已知环境的变异。表现型可塑性。后者通常归因于发育不稳定,这被认为是由于单个植物发育过程中的意外事件,与外部环境条件无关。采用遗传分析方法对株高的种内变异进行遗传分析。在7种条件下,对俄罗斯烟草(Nicotiana rustica L.) 6个自交系的完全双列杂交进行了研究。利用样地内的标准差估计株高的株际变异幅度。
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引用次数: 2
Photosynthetic efficiencies of rice under humid and dry conditions, as affected by varietal difference in leaf areal nitrogen content 湿润和干燥条件下水稻光合效率受品种叶片面积氮含量差异的影响
Pub Date : 1986-03-01 DOI: 10.1270/JSBBS1951.36.22
S. Tsunoda, M. Singh
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引用次数: 1
Overcoming Cross-incompatibility between Nicotiana tabacum L. and N.rustica L. by Test-tube Pollination and Ovule Culture 通过试管授粉和胚珠培养克服烟草与褐花的杂交不亲和性
Pub Date : 1985-12-01 DOI: 10.1270/JSBBS1951.35.429
W. Marubashi, T. Nakajima
Interspecific hybrids between Nicotiana tabacum (=) and N.rustica can not be obtained by conventional crossing because of cross-incompatibilit.y. Two techniques, test-tube pollination and ovule culture, were combined to overcome cross-incompatible combination. Whole placentae with intact ovules of N.tabacum were pollinated in vitro by dusting pollen grains of N.rustica. Developed ovules were excised from the placentae and transferred to ovule culture. Finally, 12 hybrid plants were obtained by the combination of these two techniques. The hybrid plants were intermediate between their parents in their morphological appearance and had 48 chromosomes which were the additive sum of the haploid chromosomes of the parents. They failed to produce fertile pollen and were completely self-sterile. A fertile chromosome-doubled hybrid was obtained by colchicine treatment. From the results, it is considered that the combination of two techniques, test-tube pollination and ovule culture, is useful for overcoming cross-incompatibility between distantly related species.
由于烟叶(=)与野蔷薇(N.rustica)存在杂交不亲和性,无法通过常规杂交获得种间杂种。采用试管授粉和胚珠培养相结合的方法克服了杂交不亲和组合。采用粉粒法对烟粉虱全胎盘和完整胚珠进行离体授粉。从胎盘中取出成熟的胚珠,转移到胚珠培养中。通过两种技术的组合,获得了12个杂交植株。杂交植株的形态形态介于亲本之间,有48条染色体,是亲本单倍体染色体的加和。它们不能产生可育的花粉,完全自我不育。经秋水仙碱处理,获得了可育的双染色体杂种。综上所述,试管授粉和胚珠培养相结合的方法可以有效地克服远亲种间的杂交不亲和性。
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引用次数: 18
Role of Anther on Pollen Embryogenesis in Anther Culture of Nicotiana tabacum L. 烟草花药培养中花药对花粉胚胎发生的作用。
Pub Date : 1985-12-01 DOI: 10.1270/JSBBS1951.35.390
K. Aruga, T. Nakajima
In order to define the role of anther on the pollen embryogenesis in anther culture of tobacco, the presence or absence of starch granules, amount of soluble sugars and content of free amino acids in cultured anthers were investigated through comparing with those in in vivo anthers. Starch granules in the tissues of cultured anthers disappeared rapidly, and the amount of soluble sugars also decreased soon after the onset of culture. Only a very small amount of soluble sugars was detected during the first 6 days of culture although sucrose was supplemented to the medium. On the 8th day of anther culture, the amount of soluble sugars, especially sucrose increased. It is known that pollen embryogenesis of tobacco can be induced under condition of sugar starvation in the culture medium. These results indicate that in the initial period of culture, the conchtions of the anther loculus are favorable for the induction of pollen embryogenesis. From the results of free amino acid analysis of cultured anthers, it was revealed that large amounts of glutamine and asparagine were present in cultured anthers on the 10 th day of culture when cell division leading to embryo formation began in cultured anther, although no amino acid was supplemented to the medium. It has been reported that certain amino acids such as glutamine and asparagine were essential to undergo cell dlvision leading to embryo formation from pollen grains of tobacco. Therefore, the results indicate that the amino acids required for embryo formation are supplied to pollen grains by cultured anthers. The present results revealed that the factors required for the process of pollen embryogenesis were successively provided to pollen grains by cultured anthers. It is concluded that satisfactory conditions of cultured anthers are the key points of the efficient formation of pollen embryos in anther culture of tobacco.
为了明确花药在烟草花药培养花粉胚发生中的作用,通过与体内花药的比较,研究了培养花药中淀粉颗粒含量、可溶性糖含量和游离氨基酸含量的变化。培养开始后,花药组织中淀粉颗粒迅速消失,可溶性糖含量也迅速减少。在培养的前6天,虽然培养基中添加了蔗糖,但只检测到极少量的可溶性糖。在花药培养第8天,可溶性糖的含量,尤其是蔗糖的含量增加。已知在培养基中糖饥饿条件下可诱导烟草花粉胚发生。这些结果表明,在培养初期,花药室的连接有利于花粉胚发生的诱导。从培养花药的游离氨基酸分析结果可以看出,虽然培养基中没有添加氨基酸,但在培养第10天花药开始细胞分裂并形成胚时,花药中含有大量的谷氨酰胺和天冬酰胺。据报道,某些氨基酸如谷氨酰胺和天冬酰胺是烟草花粉粒细胞分化导致胚胎形成所必需的。由此可见,胚形成所需的氨基酸由培养的花药提供给花粉粒。结果表明,花粉胚发生过程所需的因子通过培养的花药陆续提供给花粉粒。结果表明,良好的花药培养条件是烟草花药培养中花粉胚有效形成的关键。
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引用次数: 9
Increasing genetic variability in common wheat by utilization of alien cytoplasms - Cytoplasmic effects on the performance and interplant variability of the F1 and F2 generations of the cross, Triticum aestivum cv. Chinese Spring*cv. Norin 61. 利用外源细胞质增加普通小麦的遗传变异——细胞质对杂交小麦F1和F2代生产性能和株系间变异的影响。中国春天*简历。61年诺林。
Pub Date : 1985-12-01 DOI: 10.1270/JSBBS1951.37.29
P. Spetsov, K. Yonezawa, K. Tsunewaki
Using eu- and alloplasmic lines of a common wheat cultivar, Chinese Spring, and euplasmic line of cv. Norin 61, the effects of four fertile cytoplasms derived from Aegilops squarrosa, Ae. speltoides, Ae. kotschyi and Ae. vavilovii on the performance and interplant variability of 13 agronomic characters in the F1 and F2 generations of the cross between Chinese Spring and Norin 61 were investigated. The performances and interplant variabilities of both generations were little affected by any of the alien cytoplasms, but sterility increased slightly by the vavilcvii cytoplasm in the F2 generation. Transgressive segregation attributable to the nucleus × cytoplasm interaction could not be detected in the present F2, in contrast with some positive transgressive segregation observed in the F2 of the cross between two related cultivars, Norin 61 and Norin 26, under the presence of the speltoides and kotschyi cytoplasms (TSUNEWAKI et al. 1985). Neither the heterosis nor the heritability in a broad sense was affected significantly by any alien cytoplasm. The present and previous results suggest that new common wheat genotypes better adapted to an alien cytoplasm than to the wheat cytoplasm might be created step by step, using the hybrids between closely rather than distantly related cultivars.
利用普通小麦品种中国春的异质系和变异小麦的正质系。2011年,对四种可育细胞质的影响进行了研究。speltoides, Ae。kotschyi和Ae。研究了中国春与Norin 61杂交F1、F2代13个农艺性状的表现和间作变率。外源细胞质对两代的性能和间作变率影响不大,但在F2代中,异种细胞质对不育性的影响略有增加。与此相反,在两个亲缘品种Norin 61和Norin 26的杂交中,在有speltoides和kotschyi细胞质存在的情况下,在F2中观察到一些积极的海侵分离(TSUNEWAKI et al. 1985)。外源细胞质对杂种优势和广义遗传力均无显著影响。目前和以往的研究结果表明,利用近缘品种而不是远缘品种之间的杂交,可以逐步创造出更适应外源细胞质而不是小麦细胞质的普通小麦基因型。
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引用次数: 7
Notes On Extractant-Dependent Leaf Peroxidase and Malate Dehydrogenase Zymograms from Twenty Plant Species 20种植物叶片过氧化物酶和苹果酸脱氢酶酶谱研究进展
Pub Date : 1985-09-01 DOI: 10.1270/JSBBS1951.35.340
T. Endo, M. Ihara
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引用次数: 0
Correlation between Successive Yield Tests for Agronomic Characters in Sweet Potato 甘薯农艺性状连续产量试验的相关性研究
Pub Date : 1985-06-01 DOI: 10.1270/JSBBS1951.35.204
Tomohiko Yoshida
Correlation coefficients over cultivars between successive yield tests in sweet potato (Ipomoea batatas [L.] LAM.) for yield and other agronomic characters were computed from breeding records for about 20 years to evaluate the accuracy of the yield tests. The means of the correlation coefficients between years were from 0.29 to 0.79 for root yield, from 0.58 to 0.88 for dry matter%, from 0.48 to 0.65 for root size, from 0.48 to 0.69 for roots/plant, and from 0.15 to 0.58 for general appearance, etc, . The correlation coefficients were generally higher in tests at advanced stages than at early stages. It was suggested that selection at early stages should emphasize dry matter% or nematode resistance, instead of root yield.
甘薯(Ipomoea batatas)连续产量试验的相关系数[j]。[LAM.])的产量和其他农艺性状是根据大约20年的育种记录计算的,以评估产量试验的准确性。根产量、干物质%、根粗、根/株、0.15 ~ 0.58、0.15 ~ 0.58、0.29 ~ 0.79、0.58 ~ 0.88、0.48 ~ 0.65、0.48 ~ 0.69、0.15 ~ 0.58等相关系数的年际平均值。相关系数一般在晚期高于早期。建议在早期选择时应重视干物质百分比或线虫抗性,而不是根系产量。
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引用次数: 3
Varietal variations in basic vegetative phase and photosensitive phase among Japanese native cultivars of rice 日本地方稻品种营养基本期和光敏期的品种变异
Pub Date : 1985-03-01 DOI: 10.1270/JSBBS1951.35.72
Yo-ichiro Sato, Kisaburo Hayashi
The days to heading of 93 native rice cultivars collected from various locations in Japan weret examined under a short-day (30°C phytotoron, in winter, natural day-length), and a long-day (a paddy field, in summer, natural day-length and temperature) condition. From the clata, varietal variations in the periods of basic vegetative phase (BVP) as shown, by days to heading in shortday condition and in photosensitive phase (PSP) as shown by the difference in days to heading between longday and shortday conclitions were surveyed, so as to learn about the mode of differentiation of early-maturing cultivars in Japan. The cultivars tested were divisible into two groups with a long (75-90r days) and a short (38-75 days) BVP. Among the cultivars with short BVPs, the distribution of PSP, varying from O to 72 days, was trimodal. Among those from areas north of 32N in latitude, PSP decreasecl with increasing latitude of collection site. But no cor-relation between PSP and latitude was found among cultivars from the southwest islands. The early-maturing cultivars (with short BVP and short PSP) distributed throughout Japan alt*nough they were infrequent. The pattern of distribution of native rice cultivars vvith varying BVP and PSP periods seems to reflect the variations in environrnental condition anc'i cropping system under which they have been cultivated so far.
在短日照条件下(冬季30°C光照条件下,自然日长)和长日照条件下(夏季水田条件下,自然日长和温度条件下),研究了从日本不同地点收集的93个本地水稻品种的抽穗天数。从这些资料中,调查了品种在基本营养期(BVP)、短日照条件下日数到抽穗期(PSP)和长日照条件下与短日照条件下日数到抽穗期(PSP)的变异,以了解日本早熟品种的分化模式。试验品种分为长(75 ~ 90天)和短(38 ~ 75天)两组。在BVPs较短的品种中,PSP在0 ~ 72 d呈三峰分布;在纬度32N以北地区,PSP随采集地点纬度的增加而降低。但在西南岛屿品种中,PSP与纬度没有相关性。早熟品种(短BVP和短PSP)在日本各地均有分布,但并不常见。不同BVP和PSP时期的本土水稻品种的分布格局似乎反映了它们迄今为止所种植的环境条件和种植制度的变化。
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引用次数: 4
Varietal Screening of Compatibility Types Revealed in F1 Fertility of Distant Crosses in Rice 水稻远缘杂交F1育性配合力的品种筛选
Pub Date : 1984-09-01 DOI: 10.1270/JSBBS1951.34.304
H. Ikehashi, H. Araki
Reproductive barriers between distant groups of rice are anticipated to be overcome through a systematic use of widely compatible varieties in breeding programs. For this purpose a total of 74 varieties were screened for compatiblity types in terms of F1 fertility by crossing them to one each of indica and japonica testers. The compatibility of a variety to a tester was rated by pollen and spikelet fertility of the F1 hybrid. The pollen fertility of more than 90 percent and the spikelet fertility of 75-80 percent were rated normal. The compatibility tests showed that the pollen fertility is independent of the spikelet fertility. Therefore, each variety was inspected for four fertility scores, namely, the pollen and the spikelet fertility in each cross to an indica and a japonica tester. Out of 24 Indonesian upland varieties, 15 showed normal pollen fertility with both testers, and normal spikelet fertility with a japonica tester, but gave remarkable spikelet sterility with an indica tester. Six showed semi-sterility in all the four scores. Only one variety, Padi Bujang Pendek revealed normal fertility in all the four scores. The remaing two seemed to belong to an exceptional type. A total of 27 Aus varieties showed many types of compatibility including six varieties of indica type and five of japonica. Five varieties revealed sterility in all the four scores. Only two, Aus 373 and Dular seemed to be widely compatibe. The remaining nine were not classified into any difinite categories. In the test of 15 varieties which are identified tolerant of salinity, drought or peat soil, ten of strongly photoperiod-sensitive varieties were classified into typical indica rice. Two photoperiod-nonsensitiwe varieties were clearly identified to be japonica. In additional tests of some varieties which are notable from previous works, Calotoc, CPSLO 17 and Ketan Nangka were confirmed to be widely compatible. The screening results indicate that the Aus group of rice is a complex of various compatibility types, while a majority of Indonesian upland rices seemed to be of a type which is closer to japonicas rather than iledicas. Only a few varieties were identified as the wide-compatibility type, contrary to the expectation based on earlier works. It was discussed that the upland cultivation has permitted the Aus group to preserve the diverse compatibility types, while photoperiod-sensitive lowland varieties are predominantly of iledica type.
通过在育种计划中系统地使用广泛相容的品种,可望克服远隔水稻群体之间的生殖障碍。为此,通过将74个品种与籼稻和粳稻各杂交1个,从F1育性方面筛选了亲和型。一个品种与一个测试者的亲和性是由花粉和小穗的育性来评定的。花粉育性在90%以上,小穗育性在75 ~ 80%为正常。配合力试验表明,花粉育性与小穗育性无关。因此,对每个品种进行了四个育性评分,即每个杂交的花粉和小穗育性,分别对一个籼稻和一个粳稻进行了测试。在24个印尼旱地品种中,15个品种的花粉育性与粳稻育性均正常,而用籼稻育性表现出显著的小穗不育性。6个在所有4个分数中显示半不育。只有一个品种,Padi Bujang Pendek在所有四个分数中显示正常的生育能力。剩下的两个似乎属于一种特殊的类型。27个Aus品种表现出多种亲和性,其中籼型品种6个,粳型品种5个。5个品种4个分数均表现不育性。只有两种,a373和dull似乎是广泛兼容的。剩下的九个没有被划入任何明确的类别。在15个耐盐、耐旱、耐泥炭的品种试验中,将10个强光敏感品种划分为典型籼稻品种。两个光周期不敏感品种被明确鉴定为粳稻。另外,在对前人研究成果中值得注意的几个品种的试验中,卡洛托克、CPSLO 17和克滩南卡被证实具有广泛的亲和性。筛选结果表明,水稻的Aus类群是多种亲和性类型的复合体,而印度尼西亚旱稻的大多数亲和性类型似乎更接近于粳稻而不是稻稻。只有少数品种被确定为宽相容性类型,这与早期工作的期望相反。讨论了旱地栽培使Aus类群保留了多样的配伍类型,而光周期敏感的低地品种则以iledica型为主。
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引用次数: 108
The Relationship between Seed Fertility and Style Shortening after Pollination in Chrysanthemum 菊花种子育性与授粉后花柱缩短的关系
Pub Date : 1984-06-01 DOI: 10.1270/JSBBS1951.34.156
K. Hattori
Experiments were carried out to determin the relationship between the style shortening observed shortly after pollination and the seed fertility (percent of fertile seeds to the total number of disc florets on each flower head). 1. It was found that the morphological difference existed between shortened style and unshortened style observed shortly after pollination was maintained until the seed maturation. 2. Where times of pollination were varied, both percentage of disc florets with shortened style to the total number of disc florets on each flower head and seed fertility increased with increased time of pollination. Moreover, in the disc florets without the shortened style, no seed was harvested. 3. In the case where self- and cross-pollination were made throughout the whle flowering period, it was observed that the percentage of disc florets with shortened style on each flower head and the seed fertility showed the various values, and the relationship between them was highly and positively correlated. Therefore, it was suggested that crossing for seed production could be done rapidly by observing whether the style shortened or otherwise after pollination. 4. To determine the status of the style shortening in the natural condition, observation on disc florets on each of flower head after open-pollination was carried out. In this case also, the relation between the paercentage of disc florets with shortened style to the total number of disc florets on each flower head and seed fertility was highly and positively correlated. This confirmed that the style shortening observed shortly after compatible pollination was the general phenomenon in the disc floret of chrysanthemum.
为了确定授粉后花柱缩短与种子育性(可育种子占每个花头上盘状小花总数的百分比)之间的关系,进行了实验。1. 结果表明,授粉后短花柱与未短花柱在形态上存在差异,并保持到种子成熟。2. 随着授粉时间的增加,花柱缩短的盘状小花占每个花头盘状小花总数的比例和种子的育性都增加。没有花柱缩短的盘状小花没有收获种子。3.在整个花期自花授粉和异花授粉的情况下,各花头上花柱缩短的盘状小花百分率与种子育性呈现不同的数值,两者之间呈高度正相关关系。因此,可以通过观察花柱在授粉后是否缩短来快速杂交制种。4. 为了确定自然条件下花柱缩短的状况,对花头开放授粉后每个花头上的盘状小花进行了观察。在这种情况下,花柱缩短的盘状小花百分率与每个花头盘状小花总数和种子育性呈高度正相关。这证实了亲和授粉后花柱缩短是菊花花盘小花的普遍现象。
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引用次数: 1
期刊
Japanese journal of breeding
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