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Varietal Difference of Plant Regeneration from Root Callus Tissues in Rice 水稻根愈伤组织再生植株的品种差异
Pub Date : 1984-06-01 DOI: 10.1270/JSBBS1951.34.147
T. Abe, Yuzo Futsufara
Tissue cultures were initiated from root sections excised from 5∼7 day-old seedling, in 60 varieties including various ecospecies i.e. japonica, indica, japonica-iridica hybrids, and large grain varieties (assumed to be javanica type) in rice (Oryza sativa L.) on basal Murashige and Skoog's (1962) medium containing 3.0 mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D), 2.O g/l of casein hydrolysate, and 30 g/l sucrose. Cultures were maintained on the same medium with 2.O mg/l 2, 4-D. Plant regeneration was accomplished by subculturing for 2 to 3 passages to the same basal medium with 0.02mg/l 2, 4-D and 10mg/l kinetin. Remarkable variability in plant regeneration was observed among varieties tested. All the cultures of the japonica varieties had good capacity for regeneration, while some of the cultures of indica, japonica-indica hybrids, and large grain varieties were poor. It was observed that 11 of 28 cultures in japonica, 2 of 16 in indica, and 1 of 10 in large grain varieties had relatively high potential for regeneration at more than 30%. Besides normal plant regeneration, cultures showed various responses such as massive rooting without shoot formation, retention of green pigmented region without further development, and occurence of necrotic dead tissue. Two distinct types of plant regeneration were observed, one of which was organogenesis, and other was somatic embryogenesis. Most of the cultures from the indica varieties, Chyokoto and Gaiya Dhan Tosar, initiated plantlet through somatic embryogenesis.
在含有3.0 mg/l 2,4 -二氯苯氧乙酸(2,4 -d), 2的基础Murashige和Skoog(1962)培养基上,从5 ~ 7天龄的幼苗中切除的根段开始组织培养,在60个品种中,包括不同的生态物种,如粳稻、籼稻、粳稻- itria杂交品种和大粒品种(假设是javanica型)。0克/升酪蛋白水解物,30克/升蔗糖。培养保持在与2。0 mg/l 2,4 - d。在含有0.02mg/l 2,4 - d和10mg/l激动素的相同基础培养基上继代2 ~ 3代,实现植株再生。所试品种间植株再生差异显著。粳稻品种的再生能力均较好,而籼稻、籼粳杂交种和大粒品种的再生能力较差。结果表明,粳稻28个培养体中有11个,籼稻16个培养体中有2个,大粒品种10个培养体中有1个具有较高的再生潜力,再生潜力大于30%。除了正常的植株再生外,培养还表现出多种反应,如大量生根而不形成新梢,绿色色素区域保留而不进一步发育,以及坏死死组织的发生。观察到两种不同类型的植物再生,一种是器官发生,另一种是体细胞胚胎发生。大多数籼稻品种千代和盖亚丹托萨的培养都是通过体细胞胚发生形成植株的。
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引用次数: 38
Plant Variety Protection Scheme in Japan 日本植物品种保护制度
Pub Date : 1984-03-01 DOI: 10.1270/JSBBS1951.34.104
S. Saiga
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引用次数: 0
Pollen storage of Iris ensata Thunb. in organic solvents and dry air under freezing 鸢尾花粉的贮藏。在有机溶剂和干燥空气中冷冻
Pub Date : 1983-09-01 DOI: 10.1270/JSBBS1951.33.269
T. Yabuya
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引用次数: 5
Present Conditions of Seed Multication for Conservation of Crop Genetic Resources in Japan : III. Forage Crops, and Vegetables (1) Cruciferae 日本作物遗传资源保护的种子育种现状(三)。饲料作物和蔬菜(1)十字花科
Pub Date : 1983-09-01 DOI: 10.1270/JSBBS1951.33.331
H. Namai
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引用次数: 0
Genetic Analysrs of Freld Resistance to Panicle Blast (Pyricula ory-ze CAV.) in Rice 水稻穗瘟病田间抗性的遗传分析
Pub Date : 1983-03-01 DOI: 10.1270/JSBBS1951.33.62
T. Higashi, N. Horisue, Shigeru Saito, Shinji Watanabe
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引用次数: 0
Studies on the Breeding of Varieties Having Low Sucker Productivity in Tobacco (Nicotiana tabacum L.) : III. Inheritance of Sucker Productivity in Two Varietal Crosses 烟草(Nicotiana tabacum L.)吸盘产量低的品种选育研究[j]。两个品种杂交吸盘产量的遗传
Pub Date : 1982-12-01 DOI: 10.1270/JSBBS1951.32.317
Toshio Matsuda, Michiko Sato
Inheritance of the number of ground suckers in tobacco (N. tabacum L.) was investigated by analysis of F1, F2 and F3 plants of two varietal crosses, MC × Virginia 115 and Matsukawa × Odrauma, following MATHER'S additive-dominance genetic model. Dominance genetic variance was larger than additive genetic variance in the cross between MC and Virginia 115, while additive variance was larger than dominance one in the cross between Matsukawa and Odaruma. Since narrow-sense heritability values for F3 Iine mean were 66.47 and 93.49% respectively, selection for this character is expected to be effective in F3 and F4 generations. The number of ground suckers is estimated tb be controlled by three effective genetic factors for the cross bewteen MC and Virginia 115, and by six for the cross between Matsukawa and Odaruma.
采用MATHER加性-显性遗传模型,对MC × Virginia 115和松川× Odrauma两个品种组合的F1、F2和F3植株进行遗传分析。MC与弗吉尼亚115杂交的显性遗传方差大于加性遗传方差,松川与小田间杂交的加性遗传方差大于显性遗传方差。由于F3系的狭义遗传力平均值分别为66.47和93.49%,因此该性状在F3代和F4代的选择是有效的。据估计,MC与弗吉尼亚115杂交的地上吸盘数量受3个有效遗传因子的控制,松川与小田间杂交的地上吸盘数量受6个有效遗传因子的控制。
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引用次数: 0
Notched Grains Developed by the Minute Genes of Rice / Unbalanced Growth in Floral Glumes and Caryopsis in Rice VI. 水稻微小基因发育的缺口粒/水稻颖果和颖花的不平衡生长。
Pub Date : 1982-12-01 DOI: 10.1270/JSBBS1951.32.353
K. Takeda
In the previous reports, the author has clarified that unbalanced growth in the floral glumes and caryopsis of rice results in such aberrant grains as notched or hull-cracked grains. Because the grain size of a rice plant is generally conditioned by many minor genes, it should be treated as a quantitatively inherited character. However, if the major genes controlling grain size are available, analysis of their action may offer more simple and precise understanding about the genetic mechanism of grain development. In the present experiment, the effects of minute grain genes on the length of floral glumes and the potential length of caryospsis have been investigated with the nine F2 populations (Table 1). The potential length of the caryopsis (T1) was estimated by the length of "glume-cllpped grains", upper parts of the floral glumes were clipped-off a few days after the anthesis to allow the development of caryopsis without restriction by the size of floral glumes, while the inner length of floral glumes (C1) was indicated by the length of "non-treated gralns" which naturally developed in a state encased in the floral glumes. Without exception, T1 was longer than C1, and the T1/C1 ratio varied from ca. 1.O to larger than 1.7 in the 1, 483 F2 plants examined.
在之前的报道中,作者已经阐明了水稻颖果和花颖果的不平衡生长导致了缺口粒和裂壳粒等异常粒的产生。由于水稻植株的晶粒大小通常由许多次要基因决定,因此应将其视为数量遗传性状。然而,如果掌握了控制晶粒大小的主要基因,对其作用的分析将有助于对晶粒发育的遗传机制有更简单和精确的认识。本实验以9个F2群体为研究对象,研究了小粒基因对颖花颖片长度和核果潜在长度的影响(表1)。颖果的潜在长度(T1)是通过“夹颖粒”的长度来估计的,颖果的上部颖片在开花后几天被剪掉,使颖果的发育不受颖花颖片大小的限制。而花颖片的内部长度(C1)由“未处理粒”的长度表示,这些“未处理粒”在花颖片的包裹状态下自然发育。T1均比C1长,T1/C1比值约为1。在被检查的1483个F2电站中,0到大于1.7。
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引用次数: 1
Reconstruction of a Linkage Group Corresponding to the NISHIMURA'S Second Chromosome in Rice, Oryza sativa L. 水稻NISHIMURA第二染色体连锁群的重建
Pub Date : 1982-09-01 DOI: 10.1270/JSBBS1951.32.232
Shigetoshi Sato, K. Muraoka, Y. San
The relationships between known three linkage groups, the sixth, the ninth, and the twelfth, and chromosomes in rice (Oryza sativa L.)were reexamined by using isogenic lines of Taichung 65 with marker genes and translocation stocks. The marker genes, ghl (gold hull) and d1 (daikoku-type dwarf) included in the sixth linkage group, nl1 (neck leaf) in the ninth, and gl1 (glabrous) in the twelfth group were all found to be located on the second chromosome. Linkabae order of these marker genes and seven interchange breakpoints on this chromosome was established as gh1-d1-2-3-2-3c-nl1-2-6a-2-7a-2-3b-2-lOa-2-3d-gl1 Consequently, two more linkabae groups remain to be established.
以台中65等基因系为材料,利用标记基因和易位亲本,对水稻已知的第6、第9和第12 3个连锁群与染色体的关系进行了重新分析。第6连锁群中的标记基因ghl(金壳)和d1(大国型矮秆),第9连锁群中的nl1(颈叶)和第12连锁群中的gl1(无毛)均位于第2染色体上。这些标记基因的Linkabae序列和该染色体上的7个交换断点被建立为gh1-d1-2-3-2-3c-nl1-2-6a-2-7a-2-3b-2-lOa-2-3d-gl1。因此,还有两个Linkabae群有待建立。
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引用次数: 7
Difference in Micromorphological Pattern on Pollen Surface of Japanese Pear Cultivars 日本梨品种花粉表面微形态的差异
Pub Date : 1982-06-01 DOI: 10.1270/JSBBS1951.32.123
N. Matsuta, M. Omura, T. Akihama
The dry pollen surface of Japanese pear cultivars was investigated using the scanning electron microscope (SEM). Ridges, perlorations and nanoprocesses were observed on the exine, and the micromorphological pattern combined with these characteristics appeared to vary with the cultivars. Four cultivars, Imamuraaki, Suisei, Kikusui and Choju which apparently differed from each other with regard to the micromorphology of pollen surace, were chosen for estimating the morphological differences quantitatively. Twelve characteristics could be selected and measured on 50 pollen grains in each cultivar, based on microphotographs. Discriminant analysis was carried out using these 12 characteristics. Of the total of 200 pollen grains examined, about 76 percent could be correctly assigned to each cultivar. It is thus suggested that the micromorphology of pollen surface is available for distinguishing and identifying Japanese pear cultivars.
利用扫描电镜(SEM)对日本梨品种的干花粉表面进行了研究。不同品种的水稻外壁存在脊状突起、气孔和纳米状突起等微观形态特征。选取在花粉表面微形态上存在明显差异的今村明、瑞成、菊水和秋州4个品种,定量估算其花粉表面微形态差异。每个品种的50粒花粉,通过显微照片可以选择和测定出12个性状。利用这12个特征进行判别分析。在被检测的200个花粉粒中,大约76%可以正确地分配给每个品种。因此,花粉表面的微观形态可以作为日本梨品种的鉴别依据。
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引用次数: 9
Change of Heading Time by Transfer of Seeding Time in Japanese Paddy Rice Cultivars : I. Influence of Natural Day Length 日本水稻品种播种期转移对抽穗时间的影响:1 .自然日照长度的影响
Pub Date : 1982-06-01 DOI: 10.1270/JSBBS1951.32.171
T. Takita
The effect of natural day length on heading time in Japanese rice (O. sativa L.) cultivars has not yet completely been made clear, because of the difficulty in eliminating the effect of temperature. Five cultivars were grown under two constant day lengths of 20 and 50 minutes shorter than the day length at the summer solstice, 15 hours and 5 minutes. These day length corresponded to those of July 20 th. and August 10 th. respectively. Growth duration from seeding to heading in those day length was shorter than that in the day length at around the summer solstice.
由于温度的影响难以消除,自然日照长度对日本水稻品种抽穗时间的影响尚未完全清楚。5个品种在比夏至日长15小时5分钟短20和50分钟的2个恒定日长条件下生长。这些日长与7月20日相符。8月10日。分别。从播种到抽穗的生长时间,在这两个日照时段均短于夏至前后的日照时段。
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引用次数: 2
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Japanese journal of breeding
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