{"title":"Histological Observations on Plant Regeneration in Rice(Oryza sativa L.)Calli.","authors":"A. B. Mendoza, Y. Futsuhara","doi":"10.1270/JSBBS1951.42.33","DOIUrl":"https://doi.org/10.1270/JSBBS1951.42.33","url":null,"abstract":"","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115117906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey of Isozyme Genes by Polyacrylamide Gel Electrophoresis in Cauliflower, Broccoli and Cabbage (Brassica oleracea)","authors":"S. Gotoh, H. Ikehashi","doi":"10.1270/JSBBS1951.42.23","DOIUrl":"https://doi.org/10.1270/JSBBS1951.42.23","url":null,"abstract":"","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115292048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-06-01DOI: 10.1270/JSBBS1951.41.353
Tomohiko Ushiyama, Takao Shimizu, T. Kuwabara
Thirty-nine genotypes of maize (Zea mays L.) and one genotype of teosinte (Zea mays ssp. mexicana ) were investigated for the production of haploid plants in common wheat (Triticum aestivum L. var. Nishikazekomugi). The method to product them was followed: the detached wheat spikes pollinated by teosinte or maize genotypes were cultured in a solution of 100mg/l 2, 4-dichlorophenoxyacetic acid, 10ml/l ethanol, 0.8ml/l sulfurous acid and 40g/l sucrose for 16 days, and subsequently excised embryos were cultured on B5 medium. Wheat × teosinte cross was the highest in frequency of embryo obtained (38.5%) and in haploid frequency per pollinated florets (31.5%). It is suggested that teosinte can be used effectively in haploid breeding programs of wheat employing intergeneric crosses.
玉米(Zea mays L.)的39个基因型和大刍草(Zea mays ssp.)的1个基因型。对普通小麦(Triticum aestivum L. var. Nishikazekomugi)单倍体植株进行了研究。制备方法:将大刍草或玉米基因型授粉的离体小麦穗在100mg/l 2,4 -二氯苯氧乙酸、10ml/l乙醇、0.8ml/l硫酸和40g/l蔗糖的溶液中培养16 d，取胚在B5培养基上培养。小麦与大刍草杂交获得胚的频率最高(38.5%)，单小花单倍体频率最高(31.5%)。因此，大刍草可以有效地用于小麦单倍体的属间杂交育种。
{"title":"High frequency of haploid production of wheat through intergeneric cross with teosinte.","authors":"Tomohiko Ushiyama, Takao Shimizu, T. Kuwabara","doi":"10.1270/JSBBS1951.41.353","DOIUrl":"https://doi.org/10.1270/JSBBS1951.41.353","url":null,"abstract":"Thirty-nine genotypes of maize (Zea mays L.) and one genotype of teosinte (Zea mays ssp. mexicana ) were investigated for the production of haploid plants in common wheat (Triticum aestivum L. var. Nishikazekomugi). The method to product them was followed: the detached wheat spikes pollinated by teosinte or maize genotypes were cultured in a solution of 100mg/l 2, 4-dichlorophenoxyacetic acid, 10ml/l ethanol, 0.8ml/l sulfurous acid and 40g/l sucrose for 16 days, and subsequently excised embryos were cultured on B5 medium. Wheat × teosinte cross was the highest in frequency of embryo obtained (38.5%) and in haploid frequency per pollinated florets (31.5%). It is suggested that teosinte can be used effectively in haploid breeding programs of wheat employing intergeneric crosses.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1991-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126821355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-06-01DOI: 10.1270/JSBBS1951.41.341
Y. Kageyama, H. Fukuoka, Koji Yamamoto, G. Takeda
A Mutation causing the abnormal development of grains was induced in rlce (Oryza sativa L.) by means of the tetraploid-irradiation technique. The grain was about half of the size of a normal brown rice and consisted entirely of the embryonic tissue. Microscopic observations revealed that the endosperm cells stopped dividing at early developmental stage, while the embryo continued growing into a large cellular clump in which a plumule, a radicle and a scutellum differentiated. We termed these grains endospermless grains. On the basis of the segregation ratios of endospermless grains, it is concluded that a recessive allele on a single locus or a deletion of small chromosomal segment is resposible for the abnormal development of grains.
{"title":"The rice plant bearing endospermless grains : a novel mutant induced by gamma-irradiation of tetraploid rice (Oryza sativa L.)","authors":"Y. Kageyama, H. Fukuoka, Koji Yamamoto, G. Takeda","doi":"10.1270/JSBBS1951.41.341","DOIUrl":"https://doi.org/10.1270/JSBBS1951.41.341","url":null,"abstract":"A Mutation causing the abnormal development of grains was induced in rlce (Oryza sativa L.) by means of the tetraploid-irradiation technique. The grain was about half of the size of a normal brown rice and consisted entirely of the embryonic tissue. Microscopic observations revealed that the endosperm cells stopped dividing at early developmental stage, while the embryo continued growing into a large cellular clump in which a plumule, a radicle and a scutellum differentiated. We termed these grains endospermless grains. On the basis of the segregation ratios of endospermless grains, it is concluded that a recessive allele on a single locus or a deletion of small chromosomal segment is resposible for the abnormal development of grains.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"124 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1991-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120918563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C02 gas was supplied at a high concentration (3-5%) to the tissue culture chamber and its effect on shoot regeneration was examined in anther- and seed-derived calli of rice (Oryza sativa L.). The applicaiton of C0a2 was effective in both shoot and root regeneration. Furthermore, C02 supply reduced the necrosis of callus. When test tubes were capped with porous film in stead of aluminum foil, the regeneration rate was increased, in particular when C02 gas was supplied. Even when C02 was supplied, the addition of 3 % sucrose in the medium was more effective for the regeneration than 1% sucrose. By the C02 supply, plant regeneration was accelerated and the regenerated shoots became so vigorous that they could be safely and easily transplanted to soil.
{"title":"Effects of C02 Gas Supply on Shoot Regeneration in Rice Callus","authors":"A. Yomoda, K. Hinata","doi":"10.1270/JSBBS1951.41.41","DOIUrl":"https://doi.org/10.1270/JSBBS1951.41.41","url":null,"abstract":"C02 gas was supplied at a high concentration (3-5%) to the tissue culture chamber and its effect on shoot regeneration was examined in anther- and seed-derived calli of rice (Oryza sativa L.). The applicaiton of C0a2 was effective in both shoot and root regeneration. Furthermore, C02 supply reduced the necrosis of callus. When test tubes were capped with porous film in stead of aluminum foil, the regeneration rate was increased, in particular when C02 gas was supplied. Even when C02 was supplied, the addition of 3 % sucrose in the medium was more effective for the regeneration than 1% sucrose. By the C02 supply, plant regeneration was accelerated and the regenerated shoots became so vigorous that they could be safely and easily transplanted to soil.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114636960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somatic embryogenesis and plant regeneration were studied in 12 genotypes of the facultative apomictic species Panicum maximum Jacq. (Guineagrass). Embryogenic callus was initiated from immature embryos (0.5∼1.0mm long) on MS medium (MURASHIGE and SKOOG, 1962) supplemented with 10mg1-1 2, 4-dichlorophenoxyacetic acid (2, 4-D), 10% coconut water and solidified with 0.8% Agar. Initially various types of callus were obtained and embryogenic responses were found to be correlated with the genotypes investigated. Somatic embryos could be induced from all genotypes except one, but varieties "Petrie" and "Gatton" appeared to have the highest embryogenic capacity among the twelve genotypes. For somatic embryo germination and plant formation MS medium supplemented with GA3 and cytokinins was used. The best response was observed with 1.0mg1-1 GA3 and 1.0mg1-1 kinetin. The twelve genotypes analyzed can be classified into three groups by the frequency of somatic embryo formation and the degree of apomixis. The first group consists of highly apomictic genotypes with a high embryogenic capacity-"Petrle" and "Gatton" The second group is composed of highly apomictic types with lower tendency to form somatic embryos- "Natsuyutaka" N68/84-1-s 6, N68/84-1-s 7 N68/84-1-o 6, N68/84-1-o 8 and N68/96-8-o 10. The third group is formed by types displaying a lower tendency both in apomixis and formation of somatic embryos-S67, N68/84-1, N68/96-8, N68/96-8-o 1.
{"title":"High Frequency Somatic Embryo Formation in Cultures of Immature Embryos of Guineagrass, Panicum maximum JACQ","authors":"R. Akashi, T. Adachi","doi":"10.1270/JSBBS1951.41.85","DOIUrl":"https://doi.org/10.1270/JSBBS1951.41.85","url":null,"abstract":"Somatic embryogenesis and plant regeneration were studied in 12 genotypes of the facultative apomictic species Panicum maximum Jacq. (Guineagrass). Embryogenic callus was initiated from immature embryos (0.5∼1.0mm long) on MS medium (MURASHIGE and SKOOG, 1962) supplemented with 10mg1-1 2, 4-dichlorophenoxyacetic acid (2, 4-D), 10% coconut water and solidified with 0.8% Agar. Initially various types of callus were obtained and embryogenic responses were found to be correlated with the genotypes investigated. Somatic embryos could be induced from all genotypes except one, but varieties \"Petrie\" and \"Gatton\" appeared to have the highest embryogenic capacity among the twelve genotypes. For somatic embryo germination and plant formation MS medium supplemented with GA3 and cytokinins was used. The best response was observed with 1.0mg1-1 GA3 and 1.0mg1-1 kinetin. The twelve genotypes analyzed can be classified into three groups by the frequency of somatic embryo formation and the degree of apomixis. The first group consists of highly apomictic genotypes with a high embryogenic capacity-\"Petrle\" and \"Gatton\" The second group is composed of highly apomictic types with lower tendency to form somatic embryos- \"Natsuyutaka\" N68/84-1-s 6, N68/84-1-s 7 N68/84-1-o 6, N68/84-1-o 8 and N68/96-8-o 10. The third group is formed by types displaying a lower tendency both in apomixis and formation of somatic embryos-S67, N68/84-1, N68/96-8, N68/96-8-o 1.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128176065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-12-01DOI: 10.1270/JSBBS1951.40.469
G. Honda, Y. Koezuka, M. Tabata
Inheritance of the color and the hardness of fruits was investigated in the progenies of intercrosses between two varieties, Egoma and Shiso, which belong to Perilla frutescens. The results of experiments showed that the fruit color (white vs. brown) is controlled by an incomplete dominant gene W, which is responsible for the thickening of the epidermal cell wall of pericarp. On the other hand, the hard fruit proved to be due to duplicate genes, T1 and T2, which cause the unusual thickening of the stone cell layer in pericarp.
{"title":"Genetic studies of fruit color and hardness in Perilla frutescens.","authors":"G. Honda, Y. Koezuka, M. Tabata","doi":"10.1270/JSBBS1951.40.469","DOIUrl":"https://doi.org/10.1270/JSBBS1951.40.469","url":null,"abstract":"Inheritance of the color and the hardness of fruits was investigated in the progenies of intercrosses between two varieties, Egoma and Shiso, which belong to Perilla frutescens. The results of experiments showed that the fruit color (white vs. brown) is controlled by an incomplete dominant gene W, which is responsible for the thickening of the epidermal cell wall of pericarp. On the other hand, the hard fruit proved to be due to duplicate genes, T1 and T2, which cause the unusual thickening of the stone cell layer in pericarp.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114740857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-12-01DOI: 10.1270/JSBBS1951.40.397
H. Nakai, S. Kuwahara, Shigemasa Senga
An induced mutant of rice (designated as M57) showing a quantitative resistance to the Japanese differential races II, III and IV of bacterial leaf blight (BLB) and a qualitative resistance to the races I and V was crossed with the mother variety Harebare which is susceptible to BLB. The results of genetic analysis for the resistance using the F1 and F2 plants derived from the cross and the parents suggested that the quantitative resistance was conditioned by polygenes or some minor gene(s) and the qualitative resistance by single dominant genes. However, it was estimated through an allelism test that the qualitative resistance to the races I and V was associated with pollen contamination from the variety Shizutama carrying the BLB resistance genes Xa-1 and Xa-12. The qualitative resistance genes in the F2 and F3 tests were not found to be linked with the quantitative resistance genes, though the genes were likely to modify, to some extent, the expression of the relevant genes for the quantitative resistance to the races II, III and IV. It was also observed that the induced genes expressing a quantitative resistance to the races II, III and IV were operative for the races I and V, too. A negative trait for practical use, e.g. dwarfism of the M57 mutant was not linked to the quantitative resistance. In the F 3 progeny lines, some lines with a quantitative resistance to all the differential races and with a normal plant type like that of the mother variety Harebare, were selected, as they may become useful materials for the cross breeding for BLB resistance.
{"title":"Genetic analysis of an induced mutant of rice for a quantitative resistance to bacterial leaf blight","authors":"H. Nakai, S. Kuwahara, Shigemasa Senga","doi":"10.1270/JSBBS1951.40.397","DOIUrl":"https://doi.org/10.1270/JSBBS1951.40.397","url":null,"abstract":"An induced mutant of rice (designated as M57) showing a quantitative resistance to the Japanese differential races II, III and IV of bacterial leaf blight (BLB) and a qualitative resistance to the races I and V was crossed with the mother variety Harebare which is susceptible to BLB. The results of genetic analysis for the resistance using the F1 and F2 plants derived from the cross and the parents suggested that the quantitative resistance was conditioned by polygenes or some minor gene(s) and the qualitative resistance by single dominant genes. However, it was estimated through an allelism test that the qualitative resistance to the races I and V was associated with pollen contamination from the variety Shizutama carrying the BLB resistance genes Xa-1 and Xa-12. The qualitative resistance genes in the F2 and F3 tests were not found to be linked with the quantitative resistance genes, though the genes were likely to modify, to some extent, the expression of the relevant genes for the quantitative resistance to the races II, III and IV. It was also observed that the induced genes expressing a quantitative resistance to the races II, III and IV were operative for the races I and V, too. A negative trait for practical use, e.g. dwarfism of the M57 mutant was not linked to the quantitative resistance. In the F 3 progeny lines, some lines with a quantitative resistance to all the differential races and with a normal plant type like that of the mother variety Harebare, were selected, as they may become useful materials for the cross breeding for BLB resistance.","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127502735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-12-01DOI: 10.1270/JSBBS1951.40.537
Mamoru Suzuki
{"title":"New Summer Crop Varieties Registered by the Ministry of Agriculture : Forestry and Fisheries in 1990 : Paddy rice, Sweet potato. Soybean and Sugar cane","authors":"Mamoru Suzuki","doi":"10.1270/JSBBS1951.40.537","DOIUrl":"https://doi.org/10.1270/JSBBS1951.40.537","url":null,"abstract":"","PeriodicalId":270267,"journal":{"name":"Japanese journal of breeding","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121496874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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