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Inheritance of stylar morphology and loss of self-incompatibility in the progenies of induced autotetraploid buckwheat 同源四倍体荞麦诱导后代花柱形态的遗传和自交不亲和的丧失
Pub Date : 1982-03-01 DOI: 10.1270/JSBBS1951.32.61
T. Adachi, T. Yabuya, T. Nagatomo
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引用次数: 5
Induction of Foliar Buds by Morphactin Treatment of Flower:Bud of Pineapple (Ananas comosus MERR.) Morphactin处理菠萝花芽诱导叶芽的研究
Pub Date : 1981-09-01 DOI: 10.1270/JSBBS1951.31.261
M. Kudo, Y. Koga
n order to improve the efficiency of the asexual reproduction of pineapple plant (Ananas coleaosus MERR.), morphactins, known as plant regulator-like chemicals, were applied to the flower bud. Prior to the morphactin application, the apices of plant materials were pre-treated with ethrel to enforce the synchronous induction of compound flower buds. The flower buds thus induced were subsequently treated with various concentrations of morphactin solution, at different developmental stages, and different replications of treatment. Following the morphactin treatments, a number of foliar buds was developed from the fruitlets of a syncarp. This foliar bud development is not observed in natural pineapple plant and also had not been reported in the other plants treatecl with morphactin. These foliar buds were tentatively called "buds-from-fruit" in the present paper. When morphactin at concentrations of 80 and 100ppm was applied to the mater.ials in 6 to 12 days after ethrel pre-treatment, more than 80% of the plants produced "buds-from-fruit", and the average number of "buds-from-fruit" of single plant, i. e. single syncarp, obtained langed between 8.8 to 15.7 The maximum number of "buds from-fruit" obtained on a syncarp was 32. There was a certain stage in the development of the flower bud which was found to be critical as regards the effect of morphactin activity. This stage preceded the differentiation of the syncarp into flower primordium. In addition, morphactin treatrnent also gave rise to an increase in the number of natural foliar buds such as slips and suckers, a retardation in the emergence of the compound flower bud, and a tendency for the apical leaves of the treated plant to roll up which is a typical function of morphactin activity.
为了提高凤梨植物(Ananas coleaosus MERR.)的无性繁殖效率,在花蕾上施用了植物调节剂样化学物质morphactins。在应用morphactin之前,植物材料的顶端用乙烯基预处理,以强制同步诱导复合花蕾。随后,在不同的发育阶段和不同的重复处理中,用不同浓度的morphactin溶液处理由此诱导的花蕾。经morphactin处理后,合心果的小果实发育出许多叶芽。这种叶芽发育在天然菠萝植物中未观察到,在其他植物中也未见过用morphactin处理的报道。本文暂称这些叶芽为“果实芽”。将浓度为80ppm和100ppm的morphactin作用于物质。经乙烯利预处理后6 ~ 12 d, 80%以上的植株产生了“离果芽”,单株即单个合果的平均“离果芽”数在8.8 ~ 15.7个之间,单个合果的“离果芽”数最多为32个。在花芽发育的某一阶段被发现对morphactin活性的影响至关重要。这一阶段先于合胞分化为花原基。此外,morphactin处理还使植物的叶芽如滑片、吸盘等自然芽数增加,复合花芽出现迟缓,处理植株的顶端叶片有卷起的趋势,这是morphactin活性的典型作用。
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引用次数: 1
Chrysanthemum Breeding for Resistance to White Rust 菊花抗白锈病育种研究
Pub Date : 1981-06-01 DOI: 10.1270/JSBBS1951.31.121
Takashi Yamaguchi
A series of experiments were conducted to promote chrysanthemum breeding for resistance to white rust (Puccinia horiana P. Hennings), For testing the resistance to white rust, three plants of each species and cultivar were incubated in a box kept at 17'C under 100% relative humidity and the inoculation was effected by hanging leaves with telia actively producing sporidia from the ceiling of the box. The inoculum concentration was restricted to ca. 70 sporidia per 1mm2 leaf area. Reliable criteria for grading and rating inLection were established based principally on the extent of telium formation on the fully unfolded top leaves. White rust resistance of chrysanthernum cultivars could be evaluated by this testing method at an early stage of the plants, for their reactions to white rust at the young plant stage and the flowering stage were highly correlated. Variation in resistance depending on the fungal isolates was found in the tests of 40 cultivars inoculated with 6 typical isolates in Japan, suggesting physiological specialization in the pathogen. Among 6 isolates, some showed a wide range of pathogenicity and others medium and narrow ranges. Besids the existence of unstable cultivars showing various reactions to the fungal isolates, cultivars with stable resistance to all the isolates were also found.
为促进菊花抗白锈病育种,进行了一系列试验。为检测菊花抗白锈病的能力,将每个品种和栽培品种各3株置于17℃、100%相对湿度的箱中培养,采用吊挂叶片的方式接种,箱顶有主动产孢子虫的孢子虫。接种量限制在每1mm2叶面积约70个孢子虫。主要根据完全展开的顶叶上的端部形成程度建立了可靠的分级和分级标准。由于菊花品种在幼株期和开花期对白锈病的反应高度相关,因此该检测方法可在植株早期评价品种的抗白锈病能力。在日本用6个典型菌株接种40个品种的试验中发现,不同菌株的抗性存在差异,表明病原菌存在生理特化。6个分离株中,有的致病性范围较广,有的致病性范围较窄。除了存在对真菌分离株表现出不同反应的不稳定品种外,还发现了对所有分离株都具有稳定抗性的品种。
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引用次数: 8
Genetical Studies on the Panicle Formation in Rice : I. Analysis of Component Characters of Panicle Density 水稻穗形成的遗传研究:ⅰ。穗密度组成特征分析
Pub Date : 1980-12-01 DOI: 10.1270/JSBBS1951.30.335
Sada-aki Kondo, Y. Futsuhara
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引用次数: 5
Cytological Studies in Cultivated Species of Camellia : V.Intraspecific Variation of Karyotypes in Two Species of Sect. Thea 茶树栽培种的细胞学研究:v .两种茶树组核型的种内变异
Pub Date : 1979-09-01 DOI: 10.1270/JSBBS1951.29.205
K. Kondo
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引用次数: 9
Genetical Studies on Dense and Lax Panicles in Rice : II Character Expression and Mode of Inheritance of Dense Panicle Rice 水稻密穗与松穗的遗传研究ⅱ密穗稻的性状表达与遗传方式
Pub Date : 1979-09-01 DOI: 10.1270/JSBBS1951.29.239
Y. Futsuhara, Sada-aki Kondo, H. Kitano
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引用次数: 10
In vitro Differentiation of Haploid Plants by Anther Culture in Poncirus trifoliata (L.) RAF. 三叶Poncirus trioliata (L.)花药培养单倍体植株的离体分化英国皇家空军。
Pub Date : 1979-09-01 DOI: 10.1270/JSBBS1951.29.248
T. Hidaka, Y. Yamada, T. Shichijo
Anthers of Poncirus trifoliata (L.) RAF. (trifoliate orange, 2n=18) were cultured on the MURASHIGE and SKooG's (1962) medium containing 0.2 and/or 2.0mg/l of 2, 4-dichlorophenoxyacetic acid (2, 4-D), indole-3-acetic acid (IAA), oi-naphthaleneacetic acid (NAA), and kinetin in combination. Three weeks after planting, heart-shaped and cotyledonary embryoids appeared from the anthers. Embryoids were formed most efifciently from the anthers of early uninu-cleate stage, and on the media containing 0.2mg/1 of IAA. The callus formation was occurred on all media and was increased by the addition of 2, 4-D. Roots were induced by the transfer of the embryoids from the induction media con-taining growth regulators to the medium lacking growth regulators. From microscopic observation, some of these plantlets showed 9(x) chromosomes of haploid state and some of them had 10 (x+1), 11 (x+2), and 18 (2x) chromosomes. Besides these plantlets, mixoploids which had cells with different chromosome numbers in the same preparations, such as (9, 10), (9, 10, 11), and (10, 11) were obtained
三叶Poncirus trifoliata (L.)花药英国皇家空军。(三叶橙,2n=18)在MURASHIGE和SKooG(1962)培养基上培养,培养基中分别含有0.2和/或2.0mg/l的2,4 -二氯苯氧乙酸(2,4 -d)、吲哚-3-乙酸(IAA)、油萘乙酸(NAA)和动素。种植3周后,花药中出现心形和子叶胚状体。在IAA浓度为0.2mg/1的培养基上,未育期早期花药形成胚状体的效率最高。愈伤组织的形成发生在所有培养基上,并且随着2,4 - d的添加而增加。将胚状体从含有生长调节剂的诱导培养基转移到不含生长调节剂的培养基上诱导生根。显微镜下观察,这些植株有的呈现9(x)条单倍体状态,有的呈现10 (x+1)条、11 (x+2)条和18 (2x)条染色体。此外,还获得了在相同培养基中具有不同染色体数细胞的混合倍体(9,10)、(9,10,11)和(10,11)
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引用次数: 57
Genetical Studies on Liver Esterase Isozymes of the Japanese Quail, Coturnix coturnix japonica 日本鹌鹑肝酯酶同工酶的遗传研究
Pub Date : 1978-12-01 DOI: 10.1270/JSBBS1951.28.329
T. Hashiguchi, Y. Yoshimitsu, Y. Maeda, M. Taketomi
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引用次数: 1
Interspecific cross-incompatibility due to disturbed activation of the polar nuclei by the male nucleus 种间杂交不亲和是由于雄性细胞核干扰了极核的激活
Pub Date : 1978-03-01 DOI: 10.1270/JSBBS1951.28.71
I. Nishiyama, T. Yabuno
A total of 74 interspecific reciprocal crosses was made among ten polyploid species of Avena. The development of hybrid kernels was very variable, and grouped into four types, Rd -, Rd +, D + and E - which indicate small or very small, small, mormal and empty, shrivelled kernels, respectively, as vvell as germination (+) and non-germination (-) of hybrid kernels. In a small number of crosses, however, some intermediate types of kernels were found. The results are almost completely explained in terms of a hypothesls of polar-nuclei activation by the male nucleus in double fertilization. The strength of activating stimulus of the male nucleus and reaction of the female nucleus is expressed by terms "activating value (AV)" and "response value (RV)", respectively. Activating values assigned to Avena species show a range from 0.4 to 3 where 1 to A. strigosa is standard. The degree of seed failure is closely related to the differenec between activatlng and response value which usually is represented by an activation index (AI) of the polar nuclei. The AI is c, omputed from a formula, AV/2 RV (or X100%), In a plant selfed the AI is 1/2=0.5 (or 50%), being a normal index, and resultlng in production of normal seeds. If AI deviates from 50% the development of endosperm is often arrested, and it causes the seed abortlon in the extreme case. In the present hybridizations the activation index varies greatly from 7 to 375%Expressing in round numbers less than 20, 90-30, 30-80 and more than 80% activation indexes show Rd -, Rd +, D + and E - type kernels, res-pectively. Those of intermediate-type kernels are always found on boundary zones between the four groups. In other ¥vords, it is shortly stated that interspecific crosses of Avena are only compatible when their activation indexes fall within a range from 20 to 80 % .Thus the triple fusion of the two pdlar nuclei with the male nucleus In angiosperms seems to be a sexual isolation mec.hanism and the most efffective barrier to interspecific hybridizations.
结果表明,10个凤梨多倍体种间共进行了74次种间互交。杂种籽粒发育变化很大,可分为Rd -型、Rd +型、D +型和E -型4种类型,分别表示小粒或极小粒、小粒、正常粒和空粒、萎粒,以及杂种籽粒萌发(+)和未萌发(-)。然而,在少数杂交中,发现了一些中间类型的核。这一结果几乎完全可以用双受精中雄核激活极核的假说来解释。男性核的激活刺激强度和女性核的反应强度分别用“激活值(AV)”和“反应值(RV)”来表示。Avena的激活值为0.4 - 3,其中1 - a . striigosa为标准值。种子失败的程度与激活值与响应值之间的差异密切相关,通常用极核的激活指数(AI)来表示。AI是c,由公式AV/2 RV(或X100%)计算,在植物自交的AI是1/2=0.5(或50%),是一个正常的指数,结果产生正常的种子。如果AI偏离50%,胚乳的发育往往会受阻,极端情况下会导致种子流产。在本杂交组合中,激活指数在7 ~ 375%之间变化很大,在小于20、90 ~ 30、30 ~ 80的整数和大于80%的激活指数分别为Rd -型、Rd +型、D +型和E型。中间型核通常出现在四类核的边界地带。换句话说,简单地说,只有当其激活指数在20% ~ 80%的范围内时,Avena的种间杂交才能相容。因此,在被子植物中,双核与雄核的三融合似乎是一种性隔离机制。汉性是种间杂交最有效的屏障。
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引用次数: 1
Varietal Differences in Photoperiodic and Thermal Responses of Wheat 小麦光周期和热响应的品种差异
Pub Date : 1977-03-01 DOI: 10.1270/JSBBS1951.27.57
T. Gotoh
The need for early maturing cultivars is one of the problems Lacing wheat breeders in southwestern Japan. From this point of view, several experiments were conducted to get information on the photoperiodic and thermal responses of wheat cultivars in southwestern Japan. To find the effective natural day length of wheat, an experiment was performed changing light intensity of the frrst and the last 4 hours of a 16-hour photoperiod. The experiment showed that the minimum effective light intensity in the first 4 hours is 10lux and in the Comparing these results with the intensity of the last 4 hours is 20 lux (Figs 3 and 4). morning light and evening twilight, the effective day length in Fukuyama (34° 30' N) was assumed to be the astronomical day length +49 minutes. Considering the fact that the length of twilight is inversely proportional to the peripheral velocity of the earth's surface at a given place, the effective day length and the mean temperature for wheat at heading time and one month and two months before heading time were estimated for all parts of Japan as shown in Fig. 5.
对早熟品种的需求是困扰日本西南部小麦育种者的问题之一。在此基础上,对日本西南地区小麦品种的光周期和热响应进行了研究。为了确定小麦的有效自然日照长度,进行了在16小时光周期中改变前4小时和后4小时光照强度的实验。实验结果表明,前4小时的最小有效光强为10lux,与后4小时的最小有效光强为20lux(图3和图4),晨光和黄昏,假设福山(34°30′N)的有效日长为天文日长+49分钟。考虑到某一地点的黄昏长度与地表周边速度成反比,我们对日本各地小麦抽穗时、抽穗前一个月和两个月的有效白昼长度和平均温度进行估算,如图5所示。
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引用次数: 17
期刊
Japanese journal of breeding
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