Chinese Journal of Analytical Chemistry最新文献_第10页

Analytical strategies for quantifying methylated selenium species in staple crops: Methods, and emerging techniques 主食作物中甲基化硒的定量分析策略：方法和新兴技术

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-04-01 DOI: 10.1016/j.cjac.2025.100511

Angstone Thembachako MLANGENI , Fatema JAGOT , Sydney NAMAUMBO , Noel Jabesi KAPITO , Deborah Charles TSUKULUZA , Loti BOTHA , Patrick NDOVIE , Patsani KUMAMBALA

Background

Selenium is an essential trace element that exists in various forms, including methylated species, each with distinct biological activities and toxicity profiles. Understanding these transformations is critical for evaluating selenium's role in health and the environment. This review was conducted through a targeted search of multiple databases, including PubMed, Scopus, Web of Science, and Google Scholar. Keywords such as "selenium methylation detection" and "analytical methods for selenium" were used to gather a broad and relevant selection of articles. Studies were included based on their relevance, methodological quality, and contributions to the field.

Results

The review provides a detailed analysis of both established and emerging analytical techniques for selenium detection. This includes gas chromatography-mass spectrometry (GC–MS), liquid chromatography-mass spectrometry (LC-MS), high-performance liquid chromatography (HPLC), X-ray absorption spectroscopy (XAS), and nuclear magnetic resonance (NMR). It evaluates the effectiveness of these methods in separating, identifying, and quantifying selenium species, with a particular focus on sensitivity, selectivity, and throughput. Additionally, new technologies such as capillary electrophoresis coupled with inductively coupled plasma-mass spectrometry (CE-ICP-MS) and laser-induced breakdown spectroscopy (LIBS) are discussed for their potential to enhance precision and efficiency.

Significance

This review highlights the critical role of advanced analytical methods in understanding selenium transformations and improving measurement accuracy. By addressing challenges such as matrix effects, overlapping retention times, and spectral interferences, the review underscores the importance of reliable selenium analysis for health and environmental assessments, and provides insights into the ongoing advancements in analytical technology.

硒是一种必需的微量元素，以多种形式存在，包括甲基化的形式，每种形式都具有不同的生物活性和毒性特征。了解这些转化对于评估硒在健康和环境中的作用至关重要。本综述是通过多个数据库的目标搜索进行的，包括PubMed、Scopus、Web of Science和谷歌Scholar。关键词如“硒甲基化检测”和“硒的分析方法”收集广泛和相关的文章选择。研究根据其相关性、方法质量和对该领域的贡献被纳入。结果对现有的和新兴的硒检测技术进行了详细的分析。这包括气相色谱-质谱（GC-MS），液相色谱-质谱（LC-MS），高效液相色谱（HPLC）， x射线吸收光谱（XAS）和核磁共振（NMR）。它评估了这些方法在分离、鉴定和定量硒的有效性，特别关注灵敏度、选择性和通量。此外，还讨论了毛细管电泳耦合电感耦合等离子体质谱（CE-ICP-MS）和激光诱导击穿光谱（LIBS）等新技术在提高精度和效率方面的潜力。本文综述了先进的分析方法在理解硒转化和提高测量精度方面的重要作用。通过解决诸如基质效应、重叠保留时间和光谱干扰等挑战，该综述强调了可靠的硒分析对健康和环境评估的重要性，并为分析技术的持续进步提供了见解。

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引用次数: 0

Analysis of metal electrochemical corrosion behavior and velocity under AC stray current 交流杂散电流作用下金属的电化学腐蚀行为及腐蚀速度分析

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-29 DOI: 10.1016/j.cjac.2025.100528

Jianli LI, Jun AN, Haining YU, Jing WANG

Stray currents can cause electrochemical corrosion of metals, accelerate material aging, and even pose safety hazards. By studying corrosion behavior and speed, the degree of damage caused by stray currents to metals can be evaluated, protective measures (cathodic protection, insulation design, etc.) can be optimized, the service life of metal structures can be extended, maintenance costs can be reduced, and the safe and stable operation of power systems and infrastructure can be ensured. Therefore, research on the electrochemical corrosion behavior and velocity analysis method of metals under AC stray current. This article mainly explores the influence of different alternating current (AC) stray current densities on the electrochemical corrosion behavior of 316L stainless steel. The experiment used Yingtan soil simulation solution, and analyzed the changes in indicators through electrochemical testing, corrosion morphology observation, and corrosion rate calculation. The results indicate that the corrosion rate of 316L stainless steel in soil simulation solution shows a trend of first decreasing and then increasing when disturbed by AC stray current density. In the initial stage, the synergistic effect of high concentrations of Cl^– and O₂ leads to a faster corrosion rate. Over time, corrosion products increase and form a film layer, which hinders harmful ion erosion and slows down the corrosion rate. However, after prolonged immersion, the corrosion product film may crack, crevice, or even peel off, causing crevice corrosion and galvanic corrosion, accelerating the corrosion process. AC stray current forms a "tip discharge" through the defect, further exacerbating corrosion. With the increase of AC interference current density, the corrosion rate of 316L stainless steel significantly increases, and the main corrosion form changes from uniform corrosion to localized corrosion. When the stray current density is greater than or equal to 200 A/m², the corrosion degree of 316L stainless steel under the peeling coating reaches severe corrosion. This study is of great significance for understanding the impact of AC stray currents on metal corrosion and developing effective protective measures.

杂散电流会引起金属的电化学腐蚀，加速材料老化，甚至造成安全隐患。通过研究腐蚀行为和腐蚀速度，可以评估杂散电流对金属的破坏程度，优化保护措施（阴极保护、绝缘设计等），延长金属结构的使用寿命，降低维护成本，保证电力系统和基础设施的安全稳定运行。因此，研究了金属在交流杂散电流作用下的电化学腐蚀行为及速度分析方法。本文主要研究了不同交流杂散电流密度对316L不锈钢电化学腐蚀行为的影响。实验采用鹰潭土壤模拟溶液，通过电化学测试、腐蚀形态观察、腐蚀速率计算等分析指标变化。结果表明：受交流杂散电流密度干扰时，316L不锈钢在土壤模拟溶液中的腐蚀速率呈现先减小后增大的趋势；在初始阶段，高浓度Cl -和O2的协同作用导致腐蚀速率加快。随着时间的推移，腐蚀产物增加并形成膜层，阻止有害离子的侵蚀，减缓腐蚀速度。但在长时间浸泡后，腐蚀产品膜可能出现裂纹、裂缝，甚至剥落，引起缝隙腐蚀和电偶腐蚀，加速腐蚀过程。交流杂散电流通过缺陷形成“尖端放电”，进一步加剧腐蚀。随着交流干扰电流密度的增大，316L不锈钢的腐蚀速率显著增大，主要腐蚀形式由均匀腐蚀转变为局部腐蚀。杂散电流密度大于等于200 A/m2时，剥落涂层下的316L不锈钢腐蚀程度达到严重腐蚀。该研究对了解交流杂散电流对金属腐蚀的影响，制定有效的防护措施具有重要意义。

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引用次数: 0

Electrochemical surface modification and regeneration of carbon fiber microelectrodes in deionized water 碳纤维微电极在去离子水中的电化学表面改性与再生

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-25 DOI: 10.1016/j.cjac.2025.100531

Tao FANG , Tingting WANG , Uchkun ISHIMOV , Yuan LI , Ghulam MURTAZA , Awais IHSAN , Yanbing PAN , Ruiting TIAN , Han CHENG , Xianju HUANG

The contamination, passivation, or fouling of the detection electrodes is a serious problem undermining the analytical performance of electroanalytical devices. In this paper, a simple and effective carbon fiber microelectrodes (CFME) renewal method was proposed for the first time. CFME was pretreated with an initial potential of 1.75 V in deionized water for 26.13 min to obtain an activated electrode with a significantly increased electrochemical response to dopamine. This method can restore the electrochemical performance of the inactivated CFME. An advantage of this method is that its activation and regeneration effect on the electrode was not less than the other reported activation solutions, although deionized water does not contain any other electrolyte. Our study furthered the understanding of the activation mechanism of CFME treated electrochemically in deionized water, this was ascribed to a renewing in the surface of carbon fibers after electrochemical treatment. At the same time, it was proved that the oxygen-containing functional groups were modified on the surface of activated CFME, which led to the regeneration of an electrochemically active surface and improved CFME sensitivity. The differential pulse voltammetry (DPV) response of dopamine showed good linearity (R² = 0.9961) on the regenerated CFME in the concentration range from 1.0 × 10⁻⁷ to 1.0 × 10⁻⁴ mol/L. A limit of detection as low as 3.1 × 10^–8 mol/L was found. The regenerated CFME was quite stable and had good reproducibility. It is expected to be applied to activate and regenerate electrodes contaminated in the monitoring of a complex biological microenvironment.

检测电极的污染、钝化或污垢是一个严重的问题，会破坏电分析设备的分析性能。本文首次提出了一种简单有效的碳纤维微电极（CFME）更新方法。CFME在去离子水中以1.75 V的初始电位预处理26.13 min，得到对多巴胺电化学反应显著增强的活化电极。该方法可以恢复失活CFME的电化学性能。该方法的一个优点是，尽管去离子水不含任何其他电解质，但其对电极的激活和再生效果不低于其他报道的激活溶液。我们的研究进一步了解了CFME在去离子水中电化学处理后的活化机理，这归因于电化学处理后碳纤维表面的更新。同时证明了在活化的CFME表面修饰含氧官能团，使CFME的电化学活性表面再生，提高了CFME的灵敏度。在1.0 × 10−7 ~ 1.0 × 10−4 mol/L范围内，多巴胺对再生CFME的差分脉冲伏安（DPV）响应呈良好的线性关系（R2 = 0.9961）。检出限低至3.1 × 10-8 mol/L。再生CFME稳定性好，重现性好。它有望应用于复杂生物微环境监测中被污染的电极的激活和再生。

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引用次数: 0

Characterization and Health - related Study of Maifanitum by ATR - FTIR and ICP - MS 用ATR - FTIR和ICP - MS表征美伐尼坦及其与健康相关的研究

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-19 DOI: 10.1016/j.cjac.2025.100529

Xilong QIAN , Sicheng WU , Liu ZHOU , Zheng LIU , Yanqiong PAN , Xiangsong MENG , YuLu MA , Fang FANG , Kewei WANG , Jing ZHAO , Shengjin LIU

With the continuous rise of consumers' material and physical health pursuit, it is particularly critical to evaluate the safety and effectiveness of natural healthy mineral materials such as Maifanitum. The progress of modern analytical technology enables us to deeply study the internal characteristics and mechanism of these traditional mineral materials, providing a solid scientific foundation and technical support for the application of Maifanitum. A comprehensive analysis of Maifanitum was conducted using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and inductively coupled plasma mass spectrometry (ICP-MS). The ATR-FTIR analysis established a quality control fingerprint for Maifanitum, and the similarity evaluation method was employed to assess the similarity between different samples. Orthogonal partial least squares discriminant analysis (OPLS-DA) analysis revealed distinct absorption peaks among Maifanitum samples from different production areas, enabling traceability and identification. ICP-MS technology was utilized to analyze the inorganic elements in Maifanitum, revealing numerous elements closely associated with human health. These findings indicate that the established ATR-FTIR fingerprint can be effectively used to identify and control the quality of Maifanitum. The characteristics of inorganic elements in Maifanitum can not only help to distinguish its sources, but also provide a theoretical basis for the current application of Maifanitum in health care.

随着消费者对物质和身体健康追求的不断提升，对麦芬尼姆等天然健康矿物材料的安全性和有效性进行评价显得尤为关键。现代分析技术的进步，使我们能够深入研究这些传统矿物材料的内在特性和作用机理，为麦芬石的应用提供了坚实的科学基础和技术支撑。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）和电感耦合等离子体质谱（ICP-MS）对美伐尼坦进行了综合分析。通过ATR-FTIR分析，建立了麦香菇的质量控制指纹图谱，并采用相似度评价方法对不同样品间的相似度进行评价。正交偏最小二乘判别分析（OPLS-DA）显示，不同产地的麦芬尼姆样品具有不同的吸收峰，具有可追溯性和可鉴别性。利用ICP-MS技术对麦芬尼姆中的无机元素进行了分析，揭示了许多与人体健康密切相关的元素。结果表明，所建立的ATR-FTIR指纹图谱可有效地用于鉴别和控制麦凡尼姆的质量。麦发菌中无机元素的特征不仅可以帮助区分其来源，还可以为目前麦发菌在保健中的应用提供理论依据。

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引用次数: 0

Deciphering the mechanism of Sang Ju Yin in ameliorating acute lung injury: An integrated systems pharmacology approach encompassing chemical composition analysis, network pharmacology, metabolomics, molecular docking and molecular biology 解密桑菊饮改善急性肺损伤的机制：包含化学成分分析、网络药理学、代谢组学、分子对接和分子生物学的综合系统药理学方法

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-11 DOI: 10.1016/j.cjac.2025.100527

Ruinan REN , Fang WANG , Guoan ZHAO , Qi YU , Jinling ZHANG , Wenbao WANG , Tianyang WANG , Song LIN , Yan LIN

Sang Ju Yin (SJY), a renowned traditional Chinese medicinal formula, has been widely utilized in acute lung injury (ALI) management. Yet, its underlying therapeutic mechanisms remain obscure. This study devised an innovative and integrated methodology, merging advanced chemical analysis with biological assays, to elucidate SJY's action mechanism in ALI treatment. Initially, UFLC-ESI-QTOF-MS was employed to precisely analyze SJY's chemical constituents. Subsequently, network pharmacology predicted potential targets and signaling pathways for SJY's beneficial effects. In parallel, pharmacodynamic evaluation was performed on ALI rats. Utilizing LC-MS and ¹H NMR metabolomics techniques with an innovative data fusion strategy, potential biomarkers and perturbed metabolic pathways were identified. Crucially, integrating network pharmacology and metabolomics insights yielded a holistic understanding of the mechanism. Finally, verification experiments involving molecular docking, Western Blot, and qRT-PCR were carried out. Notably, potential key bioactive components including apigenin were identified for SJY's anti-ALI activity. Marked perturbations in representative pathways such as arachidonic acid (AA) metabolism and PI3K-Akt pathway were obtained after SJY administration. Furthermore, integrated data spotlighted PTGS2, PLA2, and AA metabolism as pivotal, linking predicted targets and metabolic alterations for SJY in ALI treatment. In summary, this study uncovers SJY's mechanism in ALI treatment, presenting an advanced interdisciplinary framework. It deepens our comprehension of traditional Chinese medicine's role in ALI, setting a new standard for research at the chemistry-pharmacology interface.

桑菊饮是一种著名的中药配方，在急性肺损伤（ALI）治疗中得到广泛应用。然而，其潜在的治疗机制仍然不清楚。本研究设计了一种创新的综合方法，将先进的化学分析与生物分析相结合，以阐明SJY在ALI治疗中的作用机制。初步采用UFLC-ESI-QTOF-MS对SJY的化学成分进行了精确分析。随后，网络药理学预测了SJY有益作用的潜在靶点和信号通路。同时对ALI大鼠进行药效学评价。利用LC-MS和1H NMR代谢组学技术以及创新的数据融合策略，确定了潜在的生物标志物和受干扰的代谢途径。至关重要的是，整合网络药理学和代谢组学的见解产生了对机制的整体理解。最后进行分子对接、Western Blot、qRT-PCR验证实验。值得注意的是，SJY抗ali活性的潜在关键生物活性成分包括芹菜素。给药后，花生四烯酸（AA）代谢和PI3K-Akt通路等代表性通路受到明显干扰。此外，综合数据强调PTGS2、PLA2和AA代谢是关键，将ALI治疗中SJY的预测靶点和代谢改变联系起来。总之，本研究揭示了SJY在ALI治疗中的机制，提出了一个先进的跨学科框架。它加深了我们对中药在急性肝损伤中的作用的理解，为化学-药理学界面的研究树立了新的标准。

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引用次数: 0

A selective methane gas sensor based on SnO2 utilizing a reactive and porous substrate 利用反应性多孔衬底的SnO2选择性甲烷气体传感器

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100526

Youzhi LUO , Jiaqi GAO , Huihua FU , Shuhuan LIU , Zhongqiu HUA

Generally, selective response of metal oxide semiconductor (MOS) gas sensors is dominated by the process of gas diffusion and reactions through the sensing layers. The microstructure and surface chemical activities of MOS thin films simultaneously control the diffusion reaction process and modulate electronic conduction. Thus, it is very difficult to modulate the diffusion-reaction process just using sensing layers because gas diffusion and reaction process cannot be separated from the sensing films. Hence, the selective response of the MOS gas sensors is barely satisfactory. In this study, a novel sensor structure was proposed. The MOS sensing layer was printed on a porous ceramic substrate with a well-defined pore structure. The gas molecules must diffuse through the porous substrate before contacting the sensing layer of the MOS. Therefore, the diffusion reaction and sensing processes are separated from each other and are performed by two different parts of the sensor devices. Consequently, the selective response was significantly improved by the proposed sensor structure.

通常，金属氧化物半导体（MOS）气体传感器的选择性响应是由气体在传感层中的扩散和反应过程决定的。MOS薄膜的微观结构和表面化学活性同时控制着扩散反应过程和调节电子传导。因此，由于气体的扩散和反应过程不能与传感膜分离，仅用传感层来调节扩散-反应过程是非常困难的。因此，MOS气体传感器的选择性响应几乎不能令人满意。在本研究中，提出了一种新的传感器结构。MOS传感层被打印在具有明确孔隙结构的多孔陶瓷衬底上。气体分子在接触MOS的传感层之前必须先通过多孔衬底扩散。因此，扩散反应和传感过程是相互分离的，由传感器器件的两个不同部分执行。因此，所提出的传感器结构显著改善了选择性响应。

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引用次数: 0

Proteomic fingerprints of protein corona formation on MIL-88B(Fe)-NH2 metal-organic framework MIL-88B(Fe)-NH2金属-有机骨架上蛋白冠形成的蛋白质组学指纹图谱

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100524

Junyu YE , Yanhong LI , Yang GAO , Yiling LI , Bin XIA , Zhengguo CUI , Keming QU

When nanoparticles are introduced to living systems, they are quickly covered by a biomolecular coating referred to as the protein corona. This coating endows nanoparticles with new biorecognition properties, subsequently impacting their behavior and downstream toxic effects. Herein, the proteomic fingerprints of protein corona formation on Fe-based metal-organic framework (Fe-MOF) MIL-88B(Fe)-NH₂ exposed to plasma of the marine flatfish turbot (Scophthalmus maximus) were investigated. The formation of fish plasma protein corona on haemocompatibility of Fe-MOF were investigated. Transmission electron microscopy and flow cytometry confirmed the formation of plasma protein coronas on the Fe-MOF particles. The immunoglobulins and complement proteins were enriched on the Fe-MOF surface, whereas the lipoproteins and coagulation proteins were significantly depleted compared with their original concentrations in the crude plasma. Hemolysis induced by Fe-MOF was influenced by fish plasma protein corona formation. This study offers insights into the interaction between a representative Fe-MOF and fish plasma proteins, enabling a better understanding of the biological behavior of Fe-MOFs in the blood system.

当纳米颗粒被引入到生命系统中时，它们很快就会被一层称为蛋白质冠的生物分子涂层所覆盖。这种涂层赋予了纳米粒子新的生物识别特性，从而影响了它们的行为和下游毒性作用。本文研究了大比目鱼（Scophthalmus maximus）血浆中铁基金属有机骨架（Fe- mof） MIL-88B(Fe)-NH2蛋白冠形成的蛋白质组学指纹图谱。研究了鱼血浆蛋白冠的形成对Fe-MOF血液相容性的影响。透射电镜和流式细胞术证实在Fe-MOF颗粒上形成血浆蛋白冠状体。Fe-MOF表面的免疫球蛋白和补体蛋白富集，而脂蛋白和凝血蛋白则明显减少。Fe-MOF诱导的溶血受血浆蛋白冠形成的影响。本研究提供了具有代表性的Fe-MOF与鱼血浆蛋白之间相互作用的见解，从而更好地理解Fe-MOF在血液系统中的生物学行为。

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引用次数: 0

Sensitive determination of amphetamine-type stimulants in human hair by electro-enhanced solid-phase microextraction coupled to gas chromatography with nitrogen-phosphorus detector 电增强固相微萃取-气相色谱-氮磷检测器灵敏测定人类毛发中的苯丙胺类兴奋剂

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-06 DOI: 10.1016/j.cjac.2025.100525

Aiying SONG , Rong LIU , Xinghe HE , Linlin WEI

In the field of forensic sciences, the analysis of amphetamine-type stimulants in hair samples has become a fundamental tool for assessing long-term substance use. The persistent nature of amphetamine-type stimulants in hair provides a unique chronological record of drug intake, offering valuable insights for legal and clinical applications. Developing a simple, sensitive, and reliable method for the determination of these drugs in hair samples remains an important issue in forensic analysis. In this study, polydimethylsiloxane modified stainless steel fiber was firstly fabricated. By taking advantage of the affinity of the polydimethylsiloxane coating toward amphetamine-type stimulants and the excellent conductivity of stainless steel wire, an electro-enhanced solid-phase microextraction coupled with gas chromatography equipped with a nitrogen-phosphorus detector method was established to sensitively determine four common amphetamine-type stimulants in hair samples. The experimental results showed a linear range from 0.1 to 50 µg L⁻¹. The limits of detection and qualification respectively varied from 0.012 to 0.039 and 0.05 to 0.13 µg L⁻¹ (S/N = 3), and it exhibited good reproducibility (< 6.2%). Recoveries ranging from 79% to 106%, with a relative standard deviation of < 6.7%, were obtained for all analytes. Fiber-to-fiber reproducibility (RSD), obtained using three fibers, was < 8.8%. The developed strategy combined the affinity of the polydimethylsiloxane coating towards amphetamine-type stimulants with the electrical conductivity of the stainless steel wire, effectively improving the enrichment ability and detection sensitivity, and achieving simultaneous selective enrichment and detection of several analytes. This is a simple, efficient, and reliable method for the analysis of amphetamine-type stimulants from complex matrices.

在法医学领域，分析毛发样本中的苯丙胺类兴奋剂已成为评估长期药物使用情况的基本工具。苯丙胺类兴奋剂在毛发中的持久性为药物摄入提供了独特的时间记录，为法律和临床应用提供了宝贵的见解。开发一种简单、灵敏、可靠的方法来测定毛发样本中的这类药物仍然是法医分析中的一个重要问题。本研究首先制备了聚二甲基硅氧烷改性不锈钢纤维。利用聚二甲基硅氧烷涂层对苯丙胺类兴奋剂的亲和性和不锈钢丝良好的导电性，建立了电增强固相微萃取-气相色谱-氮磷检测器联用方法，灵敏地测定了毛发样品中四种常见的苯丙胺类兴奋剂。实验结果表明，其线性范围为 0.1 至 50 µg L-¹。检出限和定性限分别为 0.012 至 0.039 和 0.05 至 0.13 µg L-¹（信噪比为 3），重现性良好（6.2%）。所有分析物的回收率为 79% 至 106%，相对标准偏差为 6.7%。使用三根纤维获得的纤维间重现性（RSD）为 8.8%。所开发的策略将聚二甲基硅氧烷涂层对苯丙胺类兴奋剂的亲和性与不锈钢丝的导电性相结合，有效提高了富集能力和检测灵敏度，实现了对多种分析物的同时选择性富集和检测。该方法简单、高效、可靠，适用于复杂基质中苯丙胺类兴奋剂的分析。

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引用次数: 0

Development of a new eco-friendly HPLC method and assessing the pharmacokinetics of pelitinib, a potent epidermal growth factor receptor inhibitor used for the treatment of non-small cell lung cancer associated with EML4-ALK mutant gene 高效液相色谱法的建立及对培利替尼药代动力学的评价。培利替尼是一种有效的表皮生长因子受体抑制剂，用于治疗EML4-ALK突变基因相关的非小细胞肺癌

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-01 DOI: 10.1016/j.cjac.2025.100513

Rashed N. HERQASH , Ali S. ALQAHTANI , Ibrahim A. DARWISH

Pelitinib (PEL) is a potent drug which demonstrated clinical success in the treatment of non-small cell lung cancers harboring the mutant fusion gene EML4-ALK (echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase. The objective of this study was the development of eco-friendly HPLC method to refine the therapeutic findings, support pharmacokinetic assessments, and aid in the potential therapeutic monitoring of PEL. The chromatographic separation of PEL and levofloxacin as an internal standard (IS) was accomplished on a Shim-pack VP-ODS C18 HPLC column using a gradient elution with a mobile phase consisting of formic acid (0.1 %, v/v) in an acetonitrile–methanol mixture (80:20 %, v/v). The flow rate was 1.0 mL min^–1 and the detection wavelength was 260 nm. Validation of the method was conducted in accordance with ICH guidelines for bioanalytical method validation, and all parameters’ values were acceptable. The novelty of the proposed eco-friendly HPLC-UV method in terms of its adherence of the method's procedures to the requirements of the green analytical chemistry approaches was confirmed by three comprehensive metric tools. The method was successfully applied in studying the pharmacokinetics of PEL in rats. The findings revealed that PEL was absorbed and reached maximum plasma concentration of 182.08 ng mL⁻¹ after 4 h and had an eliminated rate constant of 0.072 h⁻¹. The volume of distribution was 0.064 L/kg. The clearance was found at 0.005 L/h/kg. The results presented this method as a valuable tool for realizing targeted therapeutic benefits and ensuring safety of PEL treatment.

Pelitinib （PEL）是一种有效的药物，在治疗含有突变融合基因EML4-ALK（棘皮微管相关蛋白样4-间变性淋巴瘤激酶）的非小细胞肺癌方面取得了临床成功。本研究的目的是开发环保高效液相色谱法，以完善治疗结果，支持药代动力学评估，并帮助潜在的治疗监测PEL。在Shim-pack VP-ODS C18高效液相色谱柱上，采用梯度洗脱，流动相为甲酸（0.1%,v/v），乙腈-甲醇混合物（80:20%,v/v），完成PEL和左氧氟沙星作为内标（IS）的色谱分离。流速1.0 mL min-1，检测波长260 nm。方法的验证按照ICH生物分析方法验证指南进行，所有参数值均可接受。三种综合计量工具证实了所提出的环保型HPLC-UV方法的新颖性，因为该方法的程序符合绿色分析化学方法的要求。该方法成功地应用于大鼠体内的药代动力学研究。结果表明，4 h后，PEL被吸收，血药浓度达到最大值182.08 ng mL−1，消除速率常数为0.072 h−1。分布容积为0.064 L/kg。清除率为0.005 L/h/kg。结果表明，该方法是实现靶向治疗效益和确保PEL治疗安全性的有价值的工具。

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引用次数: 0

Apoptosis-inducing effects of aqueous extract of Eleutherococcus senticosus on non-small cell lung cancer cell proliferation 刺棘球菌水提物对非小细胞肺癌细胞增殖的诱导凋亡作用

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry

Pub Date : 2025-03-01 DOI: 10.1016/j.cjac.2025.100510

Xuekun KOU , Yufeng LI , Lei WANG , Xin SONG , Dan LI , Zhuo WANG , Yuanyuan ZHAO , Xiaohui ZHANG , Jingwu LI , Zhaobin XING

Non-small cell lung cancer is a malignant tumor with high morbidity and mortality worldwide. Eleutherococcus senticosus can induce apoptosis in non-small cell lung cancer cells, but the mechanism remains unclear. This study aimed to elucidate the role of Eleutherococcus senticosus in inducing apoptosis in non-small cell lung cancer cells and analyze its potential active constituents, targets, and molecular mechanisms. The results of network pharmacology analysis showed that Eleutherococcus senticosus contained 49 active ingredients that induced apoptosis in non-small cell lung cancer cells, and these components could act on 66 apoptosis-related targets. Compared to the control group, Eleutherococcus senticosus significantly increased apoptosis in A549 cells with increasing concentration (p < 0.05). The results of transcriptome and metabolomic analyses showed that Eleutherococcus senticosus significantly changed 5836 genes and 418 metabolites in A549 cells (p < 0.05), with the most significant changes in 18 genes and 34 metabolites related to apoptosis. qRT-PCR and Western blot results showed that, after Eleutherococcus senticosus treatment, the mRNA and protein expression of EGFR, MAPK3, and ICAM1 significantly increased, while CTSK decreased (p < 0.01 or p < 0.001). Correlation analysis and molecular docking results indicated that calycanthoside and oleanolic acid can directly modify the expression levels of the transcription factors POU2F3, FOXS1, and TGIF2LY or indirectly influence the binding affinity of these transcription factors to the promoters of key target genes, ultimately leading to the activation of EGFR, MAPK3, ICAM1, and CTSK, which triggers apoptosis in non-small cell lung cancer cells.

非小细胞肺癌是世界范围内发病率和死亡率较高的恶性肿瘤。刺棘球菌可诱导非小细胞肺癌细胞凋亡，但其机制尚不清楚。本研究旨在阐明刺痛棘球绦虫在诱导非小细胞肺癌细胞凋亡中的作用，并分析其潜在的活性成分、靶点和分子机制。网络药理学分析结果显示，刺痛刺球菌含有49种诱导非小细胞肺癌细胞凋亡的活性成分，这些成分可作用于66个凋亡相关靶点。与对照组相比，随着浓度的增加，刺痛棘球绦虫显著增加A549细胞的凋亡(p <；0.05)。转录组学和代谢组学分析结果显示，在A549细胞中，有5836个基因和418种代谢物发生了显著变化(p <；0.05)，与细胞凋亡相关的18个基因和34个代谢物变化最为显著。qRT-PCR和Western blot结果显示，裸眼棘球蚴处理后，EGFR、MAPK3、ICAM1 mRNA和蛋白表达量显著升高，CTSK表达量显著降低(p <；0.01或p <；0.001)。相关分析和分子对接结果表明，花青素苷和齐墩果酸可以直接改变转录因子POU2F3、FOXS1和TGIF2LY的表达水平或间接影响这些转录因子与关键靶基因启动子的结合亲和力，最终导致EGFR、MAPK3、ICAM1和CTSK的激活，从而触发非小细胞肺癌细胞的凋亡。

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