Chinese Journal of Analytical Chemistry最新文献

{"title":"Synthesis, characterization, antioxidant and bioactivity assessment, and thermodynamic analysis of metal ion complexes using a novel azo dye","authors":"Asseel AA. Al-naemi ,&nbsp;Abbas Ali Salih Al-Hamdani ,&nbsp;Susan Duraid Ahmed ,&nbsp;Muhammad Fazle Rabbee ,&nbsp;Nokeun Park ,&nbsp;Wail Al Zoubi","doi":"10.1016/j.cjac.2025.100586","DOIUrl":"10.1016/j.cjac.2025.100586","url":null,"abstract":"<div><div>In this study, the new azo dye,5,5-[1,2-phenylenebis(2,1-biazenediyl)] bis[8-quinolino], was used to synthesize complexes with Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺, and Cd²⁺ ions. The compounds were characterized using ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, mass spectrometry, thermo gravimetric analysis (TGA), diffevential scanning calovimltry (DSC), CHN analysis. Further, conductivity, magnetic susceptibility, and metal and chlorine content analysis using FT-IR spectroscopy revealed that the ligand chelates as a bidentate (OH) phenol group and a bidentate (C<img>N) ring group. The ligand exhibited tetradentate behavior, forming tetrahedral complexes. Except for Co and Ni complexes,whichare octahedral, all of them are non-electrolytes. Air-stable complexes with distinct octahedral moieties were created in a 2:1 metal:ligand molar ratio. The effectiveness of both the compounds in inhibiting free radicals was evaluatedbased on their ability to act as antioxidants was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical and gallic acid as a standard substance. The IC₅₀ value indicated that the ligand had higher free radical inhibition ability, and the ability to inhibit the compoundsvaried.The ligand and complexes with Co²⁺, Cu²⁺, and Zn²⁺ were tested against <em>Escherichia coli, Bacillus spp, Penicillium, Aspergillus niger, Fusarium, and Trichoderma</em> at two concentrations, showing varying inhibitory effects on bacterial and fungal growth compared to the control.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100586"},"PeriodicalIF":1.3,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of simultaneous quantitative analytical method for emerging contaminants in animal-derived foods using ultra performance liquid chromatography-tandem mass spectrometry","authors":"Bolin LIU ,&nbsp;Ziyue ZHAN ,&nbsp;Qi ZHANG ,&nbsp;Yu LIU ,&nbsp;Yanyu DAI ,&nbsp;Ji'an XIE ,&nbsp;Qianwen MENG ,&nbsp;Ziwei ZHAO","doi":"10.1016/j.cjac.2025.100592","DOIUrl":"10.1016/j.cjac.2025.100592","url":null,"abstract":"<div><div>Illegal and overuse of veterinary drugs, such as antibiotics, tranquilizers, and environmental pollution caused by the widespread use of per- and polyfluoroalkyl substances (PFAS) products are two concerned topics. They have caused food safety issues and seriously threaten human health. It is urgent to increase the monitoring of residues in foods. Employing enhanced matrix removal lipid (EMR-Lipid) as solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry, a simple, rapid, highly accurate, quantitative method was developed for the determination of antibiotics, PFAS and drug in animal derived foods. The extraction solvent, cleanup method and other conditions that affect the accuracy and sensitivity of the method were optimized. Under the optimal conditions, the analytes showed good linearity within their respective concentration ranges, with linear correlation coefficients (<em>R</em>²) ranging from 0.9911 to 0.9999. The method detection limits (S/<em>N</em> = 3) were 0.025 to 5.0 µg/kg, and the limits of quantification were 0.075 to 15.0 µg/kg. At concentration levels of 1, 2, and 10 times the limit of quantification, the average spiked recoveries of the 103 analytes in different matrices were 60.0 % to 119 %, and the relative standard deviations (RSDs) were 0.042 % to 19.8 %. This developed method was successfully applied to analyze 103 emerging contaminants at trace levels in the real food sample.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100592"},"PeriodicalIF":1.3,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mineralogical-based analysis of the toxicity reduction mechanism in haematitum processing","authors":"Min LU ,&nbsp;Cheng-Cheng WANG ,&nbsp;Juan LI ,&nbsp;Jing MING ,&nbsp;Guo-Hua ZHENG ,&nbsp;Bi-Sheng HUANG ,&nbsp;Yan CAO","doi":"10.1016/j.cjac.2025.100588","DOIUrl":"10.1016/j.cjac.2025.100588","url":null,"abstract":"<div><h3>Objective</h3><div>Haematitum is a mineral medicine with a wide range of clinical uses and has been in use for &gt; 2000 years. It has a certain degree of toxicity, so it is usually processed by calcination and quenching methods to reduce its toxic side effects. It is important to clarify the processing mechanism for the further development of haematitum.</div></div><div><h3>Methods</h3><div>Haematitum and processed haematitum (calcined and vinegar-quenched haematitum) were analyzed by scanning electron microscope, zeta potential analyzer, specific surface and porosity analyzer, X-ray photoelectron spectrometer, inductively coupled plasma mass spectrometer, and advanced mineral identification and characterization system. Moreover, evaluation of haematitum toxicity and procoagulant capacity using a mice model.</div></div><div><h3>Results</h3><div>The specific surface area of haematitum decreased from 17.04 to 1.42 m²/g and the zeta potential increased from −24.00 to −16.60 mV after processing. Processed haematitum and its major constituents (except quartz) are all larger in particle size than haematitum. The toxic heavy metal elements arsenic and lead in haematitum were reduced by 16.67 % and 30.77 % after processing, respectively. Some of the arsenic (III) elements are oxidized to the much less toxic arsenic (V) elements after processing. The iron (II) elements in haematitum are also partially oxidized to iron (III) elements. High doses of haematitum were toxic to the lungs, liver, and colon of mice, and the toxicity of processed haematitum was significantly reduced. Both before and after haematitum processing had procoagulant effects, and there was no significant difference.</div></div><div><h3>Conclusion</h3><div>The mineral material properties of haematitum are altered by processing, such as surface properties, mineral composition, elemental composition, and elemental valence. These findings provide evidence that processing reduces the toxicity of haematitum without affecting the efficacy.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100588"},"PeriodicalIF":1.3,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly optical sensor for detecting bismuth(III) ions in environmental, pharmaceutical, and biological sources","authors":"Ahmed H. ALANAZI ,&nbsp;Amnah S AL-ZBEDY ,&nbsp;Refat EL-SAYED ,&nbsp;Khaled F. DEBBABI ,&nbsp;Alaa S. AMIN","doi":"10.1016/j.cjac.2025.100591","DOIUrl":"10.1016/j.cjac.2025.100591","url":null,"abstract":"<div><div>A highly responsive bulk optical sensor (optode) has been introduced for the detection of bismuth [Bi(III)]. This optode integrates sodium tetraphenylborate (NaTPB) and 2-(benzothiazolylazo)1,6-naphthalenediol (BTAND) into a plasticized polyvinyl chloride (PVC) membrane, incorporating o-nitrophenyl-octyl ether (o-NPOE) as a plasticizer. The influence of several variables was optimized, including pH, the solvent mediator base matrix, and the reagent concentration. The comparison of results with previously reported methods indicates that the proposed approach, characterized by its speed and simplicity, offers a low detection limit (2.25 ng/mL) and a favorable linear range (7.5–220 ng/mL). The sensor exhibited stability, remaining unchanged even after being stored for at least one month. The sensor was successfully regenerated using a 0.25 M nitric acid (HNO₃) solution, and it responded in a reversible manner with an RSD of &lt; 2.15 % for six replicate measurements of 125 ng/mL of Bi(III) in different membranes. The capability to accurately and consistently monitor the quantity of Bi(III) ions in pharmaceutical and environmental samples with complex matrices is facilitated by the low detection limit and superior selectivity, despite the existence of interfering anions and cations.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100591"},"PeriodicalIF":1.3,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in plasma metabolome and correlation with urinary metabolome in cardiac surgery-associated acute kidney injury using ultra high-performance liquid chromatography-high resolution mass spectrometry","authors":"Yunpeng BAI ,&nbsp;Yichun JIANG ,&nbsp;Zhenmi LIU ,&nbsp;Ting LIU ,&nbsp;Silin LIANG ,&nbsp;Feng WANG ,&nbsp;Lang ZHANG ,&nbsp;Xin LIU ,&nbsp;Linhui HU ,&nbsp;Chunbo CHEN","doi":"10.1016/j.cjac.2025.100587","DOIUrl":"10.1016/j.cjac.2025.100587","url":null,"abstract":"<div><div>Cardiac surgery-associated acute kidney injury (CSA-AKI) may be related to an increase in mortality in patients in the intensive care unit; therefore, developing potential candidate molecule is particularly important for disease prediction and early diagnosis. This exploratory study investigated the alteration of the plasma metabolome profiles and possible links with the urinary metabolome in six patients with CSA-AKI at different time points, which could screen out the differential plasma metabolites for statistical analysis and altered metabolic pathways. After performing receiver operating characteristic analysis to obtain potential candidate molecules, the differential plasma metabolites with Level 1 were used for correlation analysis with the urinary metabolome. The plasma metabolome of the AKI group could be clearly separated from that of the uninjured kidney or recovered groups, but the plasma samples from recovered and uninjured groups could not be distinguished using statistical analysis. Compared with the uninjured kidney group, significant changes in the plasma metabolome were observed in such patients with CSA-AKI, especially regarding amino acid metabolism, spermidine and spermine biosynthesis, and sulfate/sulfite metabolism. The potential candidate molecules in the plasma metabolome, such as carnitines, exhibited a significantly positive correlation, while carnitines and organic acids in the plasma were involved in the correlation with the urinary metabolome. Plasma metabolic disorders were observed when CSA-AKI occurred, and the systemic status of recovered patients returned to normal after treatment. This exploratory investigation suggests potential candidate molecules in plasma and possible links to the urinary metabolome in CSA-AKI.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100587"},"PeriodicalIF":1.3,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of recombinant anti-human CD antibodies for diabetes and cancer monitoring using flow cytometry","authors":"Ziyi YANG ,&nbsp;Xinxin XU ,&nbsp;Hua KUANG ,&nbsp;Chuanlai XU","doi":"10.1016/j.cjac.2025.100558","DOIUrl":"10.1016/j.cjac.2025.100558","url":null,"abstract":"<div><div>TBNK cells, namely T, B, Natural Killer (NK) cells, are three major types of lymphocytes in the immune system. Comprehensive analysis of cluster of differentiation (CD) antigens on TBNK cell surface, such as CD3, CD4, CD8, CD16, CD19, CD56, and CD45, contributes to precise immune cell profiling and disease progression tracking. In this study, we prepared recombinant antibodies specifically target CDs markers using the ExpiCHO-Double Plasmid system, which ensured high-yield expression and reproducibility. The antibodies were efficiently conjugated with various fluorescent dyes, including FITC, PE-Cy7, APC<img>Cy7, APC, PE, and PerCP-Cy5.5, to achieve multiplexed flow cytometry analysis of TBNK cell populations. Testing of clinical samples demonstrated that the developed antibody conjugates could effectively distinguish the difference of TBNK cell populations between healthy individuals and patients, analysis of 291 adults showed type 2 diabetes had elevated CD4⁺/CD8⁺ ratios (1.9x) versus reduced ratios in cancer (0.75x), with principal component analysis (PCA) distinguishing groups (PC1 = 47.9 %) indicating their potential for disease monitoring and therapeutic evaluation. Our results underscore the utility of novel immunodiagnostic tools for early detection and personalized management of diabetes and cancer.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 8","pages":"Article 100558"},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of uranium (VI) with neutral organic phosphorus extractants: A calorimetric and spectroscopic comparison of di(1-methylheptyl) methylphosphonate vs. tri-n-butylphosphate","authors":"Yuyu Liang ,&nbsp;Xiang Xie ,&nbsp;Qingye Zhou ,&nbsp;Shufeng Zhao ,&nbsp;Xiang Li ,&nbsp;Chenchen Yuan ,&nbsp;Zeng Huang ,&nbsp;Jun Tu ,&nbsp;Xingliang Li","doi":"10.1016/j.cjac.2025.100583","DOIUrl":"10.1016/j.cjac.2025.100583","url":null,"abstract":"<div><div>Both tri-<em>n</em>‑butyl phosphate and di(1-methylheptyl) methylphosphonate demonstrate exceptional extraction capabilities for ²³³U from irradiated thorium. While extensive literature exists regarding tri-<em>n</em>‑butyl phosphate-based extraction systems, comparative studies on di(1-methylheptyl) methylphosphonate remain notably limited. This systematic investigation provides a parallel comparison of the chemical behaviors between these two extractants under identical experimental conditions. Results reveal that the di(1-methylheptyl) methylphosphonate system exhibits enhanced hydrophobicity relative to tri-<em>n</em>‑butyl phosphate, correlating with reduced water co-extraction during the uranium recovery process. Raman spectroscopic analysis demonstrated about ∼30 cm^–1 shifts in the uranyl symmetric stretching vibration for di(1-methylheptyl) methylphosphonate complexes compared to ∼15 cm^–1 shifts of tri-<em>n</em>‑butyl phosphate complexes. This significant spectral displacement indicates stronger coordination bonding between phosphoryl oxygen of di(1-methylheptyl) methylphosphonate and uranyl ions. Isothermal titration calorimetry measurements quantified the extraction thermodynamics, yielding enthalpy changes of -12.83 kJ/mol and -11.32 kJ/mol for tri-<em>n</em>‑butyl phosphate and di(1-methylheptyl) methylphosphonate systems, respectively. Di(1-methylheptyl) methylphosphonate exhibits marginally less exothermic enthalpy despite its superior extraction efficiency. The counterintuitive observation suggests distinct thermodynamic compensation mechanisms. This likely involves more favorable entropy contributions in the di(1-methylheptyl) methylphosphonate extraction system and greater energy consumption during desolvation. This comprehensive comparison clarifies fundamental differences in extraction mechanisms between tri-<em>n</em>‑butyl phosphate and di(1-methylheptyl) methylphosphonate extractants, providing critical thermodynamic parameters for optimizing ²³³U recovery processes.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100583"},"PeriodicalIF":1.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catechol-modified wet-adhesive hydrogel for the repair of the gastric epithelium","authors":"Ning Yang ,&nbsp;Yi Liu ,&nbsp;Jiannan Li ,&nbsp;Meiqi Wang ,&nbsp;Dongxin Wang ,&nbsp;Zhuo Liu","doi":"10.1016/j.cjac.2025.100581","DOIUrl":"10.1016/j.cjac.2025.100581","url":null,"abstract":"<div><div>Gastric perforation, which may be caused by damage to the gastric epithelium, can lead to the entry of food or gastric acid in the stomach into the peritoneum, causing diseases such as peritonitis and sepsis. In severe cases, it can lead to septic shock and pose a threat to life. Developing and preparing a material that has adhesiveness in a wet environment such as the surface of the stomach is a challenge. Therefore, we have successfully prepared a hydrogel membrane with wet adhesiveness. This hydrogel membrane is composed of glutaraldehyde-crosslinked polyvinyl alcohol (PVA) hydrogel modified by 3,4-dihydroxyphenylalanine (DOPA). The side chains of PVA molecules modified with DOPA achieve wet adhesion through the formation of hydrogen bonds between the catechol groups and the surface of the tissue substrate. It is used for suture-free repair of damaged gastric epithelium. According to the results of mechanical experiments, its wet adhesion lap shear strength to gastric tissue reaches 34.8 kPa, and the peeling strength reaches 24.5 kPa. The results of <em>in vitro</em> cell experiments show that the hydrogel has good biocompatibility. The hydrogel prepared by this simple method demonstrates adaptability to the gastric environment, making it promising for applications in gastric wound repair.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100581"},"PeriodicalIF":1.3,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active compounds and target genes investigation for Tongxie-Yaofang treatment in diarrhea predominant-irritable bowel syndrome based on network pharmacology study","authors":"Xiaowen Yu ,&nbsp;Shupei Ma ,&nbsp;Dongliang Zhang ,&nbsp;Xuan Chen ,&nbsp;Jun Ouyang ,&nbsp;Biao Xi ,&nbsp;Dongyu Xie ,&nbsp;Yaxiang Shi","doi":"10.1016/j.cjac.2025.100579","DOIUrl":"10.1016/j.cjac.2025.100579","url":null,"abstract":"<div><h3>Purpose</h3><div>We attempted to determine the effectiveness and dissected the detailed molecular mechanism of Tongxie-Yaofang (TXYF) in the treatment of diarrhea predominant-IBS (IBS-D) based on network pharmacology.</div></div><div><h3>Materials and methods</h3><div>Between February 2019 and December 2020, 28 IBS-D patients were included and given TXYF formula granules twice a day for consecutive 8 weeks. The efficacy of TXYF was evaluated based on IBS Symptom Severity Score (IBS-SSS), IBS-quality of life scale (QOL) and TCM syndrome score before and after 4 week, 8 week treatment. The active components of TXYF were screened and filtered by Traditional Chinese Medicine Systems Pharmacology and Traditional Chinese Medicine Integrated Database, followed by the target protein prediction. The IBD-related genes were retrieved by Comparative Toxicogenomics Database. The key proteins were intersected, and molecule docking were conducted. Finally, a quantitative real-time polymerase chain reaction (qRT-PCR) analysis based on samples obtained from IBS rat model was performed to validate the results in current bioinformatics analysis.</div></div><div><h3>Results</h3><div>TXYF treatment significantly improved IBS-SSS, IBS-QOL and TCM syndrome score after 4 and 8 week post treatment. A total of 23 active compounds, 3 herbs, and 29 key target proteins were associated with TXYF. Key target proteins, such as AKT1, PPARG, and NFKB1, were mainly enriched in pathways such as non-alcoholic fatty liver disease. The main active ingredients in TXYF for IBS-D were catechin, <em>β</em>-sitosterol, <em>β</em>-eudesmol, and Pyrethrin Ii by binding to CNR1, ESR1, TGFB1, and TNFSF11. Finally, the qRT-PCR analysis showed that hub gene TGFB2 in pharmacological network were significantly promoted in TXYF treatment group when compared with IBS blank control group, which further validated the results of our bioinformatics analysis.</div></div><div><h3>Conclusions</h3><div>TXYF may be effective in IBS-D treatment by targeting CNR1, ESR1, TGFB1, and TNFSF11. Our data may provide new clues for understanding the molecular mechanism of TXYF in IBS-D treatment.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 9","pages":"Article 100579"},"PeriodicalIF":1.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gynostemma pentaphyllum promotes strained skeletal muscle protein regeneration by adjustment of liver and spleen function via PXR-IL-6-SERCA1a","authors":"Yi Zou ,&nbsp;Xingqin Wei ,&nbsp;Guangying Wu ,&nbsp;Dongmei Li ,&nbsp;Houran Cao ,&nbsp;Weitao Chen ,&nbsp;Lingfeng Zeng ,&nbsp;Zhao Chen","doi":"10.1016/j.cjac.2025.100578","DOIUrl":"10.1016/j.cjac.2025.100578","url":null,"abstract":"<div><h3>Background</h3><div><em>Gynostemma pentaphyllum</em> (GYP) is a traditional Chinese medicine (TCM) used for strengthening and injure recovery, previous research has revealed that a 50% ethanol extract of GYP markedly influences strained skeletal muscle regeneration. Further research has clarified that GYP took such effect via recovery of liver and spleen, whose function and morphology also damaged when muscle is strained. However, the mechanism and biological basis of GYP’s regeneration effect still needs deeper investigation.</div></div><div><h3>Objective</h3><div>To elucidate the mechanism and biomarkers of GYP regulating the recovery of skeletal muscle proteins, as well as discovery of related targets involved in such process.</div></div><div><h3>Methods</h3><div>A rat blunt skeletal muscle strain model was established for pharmacodynamic evaluation, with administration of GYP extract or reference drug; the serum and tissue (liver, spleen) samples were collection for subsequent investigation of target expression and metabolomics; Serum metabolomics research was carried out to screen differential metabolites related to the skeletal muscle regeneration; Subsequently, the metabolomic results were combined with network pharmacology and results of target expression to clarify the signaling pathway; Then, guided by this, the differential metabolites related to the targets/pathways in organs such as the liver and spleen were determined by UPLC-MS; Eventually, biomarkers characterizing the promotion of protein regeneration in strained skeletal muscle by GYP were found.</div></div><div><h3>Results</h3><div>The serum metabolomic analysis successfully identified 20 differential metabolites associated with its efficacy, and further screened out metabolites like l-tryptophan, DL-glutamic acid, tyrosine, arachidonic acid as significant biomarkers that related to PXR-IL-6-SERCA1a pathway; As the regulatory mechanism of GYP indicated, those markers are recognized as key indicators of the process of strained muscle regeneration, as well as GYP’s therapeutic effect.</div></div><div><h3>Conclusion</h3><div>By restoring the function of those related organs via PXR-IL-6-SERCA1a pathway, internal TCM like GYP has good skeletal muscle recovery effect, which based on its regulation of some metabolites that plays important role in protein synthesis and inflammatory reaction in liver and spleen.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 9","pages":"Article 100578"},"PeriodicalIF":1.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}