Analytical Chemistry最新文献_第10页

A Dual-Ion Synergistic Catalysis Utilizing Zn2+-Regulated CdSySe1–y ECL Immunosensor Employed for the Ultrasensitive CA19-9 Detection 利用 Zn2+ 调节的 CdSySe1-y ECL 双离子协同催化的超灵敏 CA19-9 检测免疫传感器

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-21 DOI: 10.1021/acs.analchem.4c05034

Dawei Fan, Zhengxing Gong, Guangyue He, Hanxiao Liu, Yingli Wang, Hongmin Ma, Dan Wu, Huan Wang, Qin Wei

Carbohydrate antigen 19-9 is a well-known malignancy biomarker, and its sensitive detection is particularly crucial in the diagnosis and assessment of pancreatic cancer. In this study, an ultrasensitive CA19-9 immunosensor was constructed using the Zn²⁺-regulated CdS_ySe_1–y (Zn-CdS_ySe_1–y) nanospheres (NSs) as the electrochemiluminescence (ECL) emitter and FeCoS₂ nano octahedrons (NOs) as a coreactant enhancer. The microstructure of ternary transition metal chalcogenide CdS_ySe_1–y was precisely tuned by Zn²⁺ doping to avoid aggregation and thus enable stable and efficient cathodic ECL responses. The bimetallic sulfide FeCoS₂ was synthesized using a metal organic framework (MOF) as the template by ion permeation. It was able to catalyze the coreactant efficiently due to the synergistic effect of the Fe²⁺ and Co²⁺. The immunosensor exhibited low detection limit (7.6 × 10^–5 U mL^–1) in the wide linear range of 0.0001–100 U mL^–1, offering a sensitive CA19-9 detection method.

碳水化合物抗原19-9是一种著名的恶性肿瘤生物标记物，其灵敏检测对胰腺癌的诊断和评估尤为重要。本研究以 Zn2+ 调节的 CdSySe1-y （Zn-CdSySe1-y）纳米球（NSs）作为电化学发光（ECL）发射体，以 FeCoS2 纳米八面体（NOs）作为核反应增强剂，构建了一种超灵敏的 CA19-9 免疫传感器。通过掺杂 Zn2+ 对三元过渡金属铬化物 CdSySe1-y 的微观结构进行了精确调整，以避免聚集，从而实现稳定高效的阴极 ECL 反应。以金属有机框架（MOF）为模板，通过离子渗透合成了双金属硫化物 FeCoS2。由于 Fe2+ 和 Co2+ 的协同作用，它能有效催化核心反应物。该免疫传感器在 0.0001-100 U mL-1 的宽线性范围内表现出较低的检出限（7.6 × 10-5 U mL-1），提供了一种灵敏的 CA19-9 检测方法。

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引用次数: 0

Laser-Induced Thermophoretic SERS Enhancement on Paper for Facile Pesticide and Nanoplastic Sensing 激光诱导纸张热泳 SERS 增强，实现便捷的农药和纳米塑料传感

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-21 DOI: 10.1021/acs.analchem.4c05728

Shuang Mu, Zhaowei Tian, Wei Ren, Chenghui Liu

Surface-enhanced Raman scattering (SERS) has emerged as a powerful tool for contamination detection. Fabricating efficient nanostructures with hotspots for signal enhancement and concentrating diluted target analyte molecules to the hotspots are critical for ultrasensitive SERS detection, which generally requires advanced instruments and intricate manipulations. Herein, we report a simple, low-cost, and high-efficiency paper device that can simultaneously concentrate the analytes and generate SERS hotspots rapidly with the assistance of laser-induced thermophoresis. After dropping the target- and plasmonic nanoparticle-containing solution on a paper substrate, the evaporative gradient created by the laser-induced thermophoresis can promote the delivery of the analytes and plasmonic nanoparticles simultaneously to the tiny area of the laser spot, forming compact SERS hotspots to significantly amplify the analyte’s Raman scattering signals. This convenient thermophoretic strategy can be accomplished rapidly within ∼4 min and exhibits more than 10⁴-times higher sensitivity than that without the assistance of laser-based thermophoresis. This elegant paper device is successfully applied to the detection of contaminants such as pesticides and nanoplastics in fruit and water samples, holding the potential to provide a simple, fast, and cost-effective approach for on-site detection of environmental contaminants.

表面增强拉曼散射（SERS）已成为污染检测的有力工具。制造具有信号增强热点的高效纳米结构，并将稀释的目标分析分子集中到热点上，是超灵敏 SERS 检测的关键，而这通常需要先进的仪器和复杂的操作。在本文中，我们报告了一种简单、低成本、高效率的纸质装置，它可以在激光诱导热泳的帮助下，同时快速浓缩分析物并产生 SERS 热点。将含有目标物和等离子纳米粒子的溶液滴在纸基底上后，激光诱导热泳产生的蒸发梯度可促进分析物和等离子纳米粒子同时输送到激光光斑的微小区域，形成紧凑的 SERS 热区，从而显著放大分析物的拉曼散射信号。这种便捷的热泳策略可在∼4 分钟内快速完成，其灵敏度比没有激光热泳辅助的情况下高出 104 倍以上。这种优雅的纸质装置被成功应用于水果和水样中农药和纳米塑料等污染物的检测，有望为现场检测环境污染物提供一种简单、快速和经济有效的方法。

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引用次数: 0

A Bicyclic Dioxetane Chemiluminescence Nanoprobe for Peroxynitrite Imaging in Vivo 用于体内亚硝酸过氧化物成像的双环二氧杂环烷化学发光纳米探针

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c04510

Min Shi, Yun Zhang, Jia-Xing Chen, Yanyuan Wu, Zongping Wang, Peng-Fei Shi, Xudong Jin, Xue-Qiang Wang

Peroxynitrite (ONOO^–) is a critical biomarker associated with a wide array of diseases including cancer, inflammatory conditions, and neurodegenerative disorders. This study introduces an innovative chemiluminescence nanoprobe (CLNP) based on a bicyclic dioxetane structure, designed for highly sensitive and specific in vivo imaging of ONOO^–. Our CLNP demonstrates exceptional capabilities in generating high-contrast imaging of disease lesions, with applications verified across tumor models, acute inflammation, and acute liver injury scenarios. Key findings highlight the probe’s rapid response to oxidative species, superior tissue penetration, and high signal-to-noise ratio, underscoring its potential for real-time diagnostic applications. This work represents an important advance in the field of diagnostic imaging using CL probes, offering promising avenues for the early detection and treatment of ONOO^–-related pathologies.

过氧化亚硝酸盐（ONOO-）是一种与癌症、炎症和神经退行性疾病等多种疾病相关的重要生物标志物。本研究介绍了一种基于双环二氧杂环烷结构的创新型化学发光纳米探针（CLNP），该探针设计用于对 ONOO- 进行高灵敏度和特异性的体内成像。我们的 CLNP 在对疾病病灶进行高对比度成像方面表现出卓越的能力，其应用已在肿瘤模型、急性炎症和急性肝损伤等场景中得到验证。主要研究结果强调了该探针对氧化物种的快速反应、卓越的组织穿透性和高信噪比，突出了其在实时诊断应用方面的潜力。这项工作代表了利用CL探针进行诊断成像领域的重要进展，为早期检测和治疗ONOO相关病症提供了前景广阔的途径。

引用次数: 0

One-Pot Time-Induced Proteome Integral Solubility Alteration Assay for Automated and Sensitive Drug–Target Identification 用于自动灵敏鉴定药物靶点的单锅时间诱导蛋白质组整体溶解度改变分析法

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c05127

Zhaowei Meng, Amir Ata Saei, Hezheng Lyu, Massimiliano Gaetani, Roman A. Zubarev

The proteome integral solubility alteration (PISA) assay is widely used for identifying drug targets, but it is labor-intensive and time-consuming and requires a substantial amount of biological sample. Aiming at enabling automation and greatly reducing the sample amount, we developed one-pot time-induced (OPTI)-PISA. Here, we demonstrate OPTI-PISA performance on identifying targets of multiple drugs in cell lysate and scaling down the sample amount to sub-microgram levels, making the PISA method suitable for NanoProteomics. OPTI-PISA can be implemented using only the standard equipment of a proteomics lab.

蛋白质组整体溶解度改变（PISA）测定法被广泛用于鉴定药物靶点，但该方法耗时耗力，需要大量生物样本。为了实现自动化并大大减少样本量，我们开发了单锅时间诱导（OPTI）-PISA。在这里，我们展示了 OPTI-PISA 在鉴定细胞裂解物中多种药物靶点方面的性能，并将样品量减少到亚微克级，使 PISA 方法适用于纳米蛋白质组学。OPTI-PISA 只需使用蛋白质组学实验室的标准设备即可实现。

引用次数: 0

Unlocking the Mysteries of the Desorption–Ionization Mechanism via Separate Thermal and Charge Strategies 通过独立的热和电荷策略揭开解吸电离机制的神秘面纱

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c03823

Kaineng Huang, Yi He, Xingyu Li, Yuanjiang Pan, Yuanji Gao

Herein, a new strategy is employed to build a controllable thermal-coupled charge ionization (TCCI) device to elucidate the desorption–ionization mechanism of plasma ion sources. Efficient synergistic desorption and ionization are achieved within the TCCI device by independently controlling the desorption temperature and plasma charges. The TCCI device efficiently ionizes samples using abundant free electrons, charges, and active species from arc plasma. The coexistence of free electrons and hydroxide radicals confers redox capability to the TCCI system, implying the presence of a unified redox mechanism even when the arc plasma is transmitted through a metal conductor over a distance. In addition, molecular ions of the analytes facilitate the differentiation between primary and secondary amines during their analysis. Notably, the TCCI device enables a switch between hard and soft ionization by adjusting the thermal desorption temperature. At high temperatures (>400 °C), the TCCI device exhibits hard ionization characteristics, producing fragment ions beneficial for isomer discrimination. The TCCI mass spectrometry exhibits robust performance in terms of sensitivity and accuracy for detecting antibiotics and sterols in saline solutions, achieving linearity with correlation coefficients ≥0.99 and excellent reproducibility. The successful analysis of seven pharmaceuticals and four sterols in complex matrices using the TCCI device demonstrates its excellent salt and matrix tolerance. Overall, the TCCI device, with its independent control over thermal desorption and arc plasma, achieves efficient synergistic desorption and ionization, overcoming limitations in existing ionization technologies and contributing to the study of gas-phase ion dynamics and mechanisms.

本文采用一种新策略来构建可控热耦合电荷电离（TCCI）装置，以阐明等离子体离子源的解吸电离机制。通过独立控制解吸温度和等离子电荷，TCCI 设备实现了高效的协同解吸和电离。TCCI 设备可利用电弧等离子体中丰富的自由电子、电荷和活性物质有效地电离样品。自由电子和氢氧自由基的共存赋予了 TCCI 系统氧化还原能力，这意味着即使电弧等离子体通过金属导体远距离传输，也存在统一的氧化还原机制。此外，分析物的分子离子有助于在分析过程中区分伯胺和仲胺。值得注意的是，TCCI 设备可通过调节热解吸温度在硬电离和软电离之间切换。在高温（400 °C）下，TCCI 设备显示出硬电离特性，产生的碎片离子有利于异构体的鉴别。TCCI 质谱仪在检测生理盐水中的抗生素和甾醇时，在灵敏度和准确度方面表现出了强大的性能，实现了相关系数≥0.99 的线性和出色的重现性。使用 TCCI 设备成功分析了复杂基质中的七种药物和四种固醇，证明了其出色的耐盐性和基质耐受性。总之，TCCI 设备可独立控制热解吸和电弧等离子体，实现了高效的协同解吸和电离，克服了现有电离技术的局限性，有助于气相离子动力学和机理的研究。

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引用次数: 0

Real-Time Analysis of Lipid Droplet Morpho-Chemical Dynamics in Living Human Hepatocytes via Phase-Guided Raman Sampling 通过相位引导拉曼取样技术实时分析活体人肝细胞中脂滴的形态化学动态

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c03633

Hao Zhang, Jingde Fang, Kaiqin Chu, Zachary J. Smith

Lipid droplets (LDs) are highly dynamic organelles, undertaking many important functions such as maintaining lipid metabolism and cellular homeostasis. Traditional methods to analyze LD dynamics focus on morphological changes, while chemical dynamics cannot be easily probed with traditional analytical chemistry techniques. To overcome this challenge, we show here how our phase-guided Raman sampling method, where high-resolution phase microscopy images direct a Raman sampling beam, can perform label-free, multimodal characterization of LD dynamics in living cells at both the single-cell and single-LD levels with submicron accuracy and high temporal resolution. We demonstrate the study of the morphological–compositional dynamics of human hepatocellular carcinoma cells (PLC cells) under different environmental conditions and with and without fatty acid supplementation, providing insight into LD heterogeneity and heterogeneity of response. Finally, we introduce a measurement method for the dynamics of cell-average LD composition, which can quickly and accurately characterize the lipid dynamics at the single-cell level with <30 s temporal resolution. The results here show the promise of the phase-guided Raman sampling method for dynamic morpho-chemical profiling of organelle populations.

脂滴（LDs）是高度动态的细胞器，承担着许多重要功能，如维持脂质代谢和细胞平衡。分析脂滴动态的传统方法侧重于形态变化，而化学动态则难以用传统的分析化学技术进行探测。为了克服这一难题，我们在此展示了我们的相位引导拉曼取样方法（高分辨率相位显微镜图像引导拉曼取样束）如何在单细胞和单 LD 水平上以亚微米精度和高时间分辨率对活细胞中的 LD 动态进行无标记、多模式表征。我们展示了在不同环境条件下、补充或不补充脂肪酸时人肝癌细胞（PLC 细胞）的形态-组成动态研究，从而深入了解了 LD 的异质性和反应的异质性。最后，我们介绍了一种细胞平均低密度脂蛋白组成动态的测量方法，它能以 30 秒的时间分辨率快速、准确地描述单细胞水平的脂质动态特征。研究结果表明，相位引导拉曼采样法有望用于细胞器群的动态形态化学特征描述。

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引用次数: 0

Single-Electrode Flow Cell for Electrochemiluminescent Flow Analysis 用于电化学发光流动分析的单电极流动池

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c04322

Zhiyong Dong, Yequan Chen, Shiyu Xia, Ala’a Mhmoued Abdllh Alboull, Altaf Hussain, Yu Tian, Guobao Xu

Flow injection analysis and liquid chromatography are frequently combined with electrochemiluminescence (ECL) for flow analysis. Almost all electrochemistry flow analyses employ traditional three-electrode electrochemical flow cells which have working electrode, counter electrode, and reference electrode; however, it is expensive and difficult to fabricate a traditional three-electrode electrochemical flow cell and inconvenient to renew the electrode. In this study, we have developed a single-electrode flow cell using commercially available conductive polyethylene film as the only electrode through potential differences induced by the electrode resistance for the first time. The single-electrode flow cell features a simple structure, easy renewal of the electrode, and low cost compared to the traditional three-electrode electrochemical flow cells. Taking the typical Ru(bpy)₃²⁺/oxalate ECL system as the analytical model, flow analysis of clinically important oxalate was achieved using single-electrode flow cell. A regression linear equation was obtained over the oxalate concentration ranges from 1 to 200 μM, with a detection limit of 0.92 μM. The single-electrode flow cell is promising for ECL flow analysis.

流动注射分析和液相色谱法经常与电化学发光（ECL）相结合进行流动分析。几乎所有的电化学流动分析都采用传统的三电极电化学流动池，即工作电极、对电极和参比电极；然而，制造传统的三电极电化学流动池既昂贵又困难，而且电极更新也不方便。在这项研究中，我们首次利用市售的导电聚乙烯薄膜作为唯一电极，通过电极电阻引起的电位差开发出了单电极流动池。与传统的三电极电化学流动池相比，单电极流动池具有结构简单、电极易于更新、成本低廉等特点。以典型的 Ru(bpy)32+/oxalate ECL 系统为分析模型，利用单电极流动池实现了对临床上重要的草酸盐的流动分析。在 1 至 200 μM 的草酸盐浓度范围内得到了回归线性方程，检测限为 0.92 μM。单电极流动池有望用于 ECL 流动分析。

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引用次数: 0

Logically Activatable Nanoreporter for Multiplexed Time-Phased Imaging Assessment of Hepatic Ischemia-Reperfusion Injury and Systemic Inflammation 用于肝脏缺血再灌注损伤和全身炎症的多重时相成像评估的逻辑可激活纳米报告器

IF 7.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-20 DOI: 10.1021/acs.analchem.4c04416

Tao Tang, Fushou Liu, Biao Huang, Hao Li, Wen-Ping Lin, Zhi-Jun Sun, Mingxi Zhang, Ran Cui

Hepatic ischemia-reperfusion injury (HIRI) and induced systemic inflammation is a time-dependent multistage process which poses a risk of causing direct hepatic dysfunction and multiorgan failure. Real-time in situ comprehensive visualization assessment is important and scarce for imaging-guided therapeutic interventions and timely efficacy evaluation. Here, a logically activatable nanoreporter (termed QD@IR783-TK-FITC) is developed for time-phase imaging quantification of HIRI and induced systemic inflammation. The nanoreporters could be used for in vivo ratiometric NIR-IIb fluorescence sensing of reactive oxygen species (ROS), which can depict the in situ hepatic ROS fluctuation for the early diagnosis of HIRI in the initial 3 h. Meanwhile, the ROS-specific reaction releases renal-clearable fluorophore fragments from nanoreporters for monitoring the systematic inflammation induced by HIRI via longitudinal urinalysis. In addition, a functional relationship between digitized signal outputs (NIR-IIb ratios, urinary fluorescence) with hepatic injury scores has been established, realizing precise prediction of HIRI severity and preassessment of therapeutic efficacy. Such a time-phased modular toolbox can dynamically report HIRI-induced systemic inflammation in vivo, providing an efficient approach for HIRI treatment.

肝脏缺血再灌注损伤（HIRI）和诱发的全身炎症是一个时间依赖性的多阶段过程，有直接导致肝功能障碍和多器官功能衰竭的风险。实时原位综合可视化评估对于成像引导的治疗干预和及时的疗效评估非常重要，也非常稀缺。在此，我们开发了一种逻辑上可激活的纳米报告器（称为 QD@IR783-TK-FITC），用于对 HIRI 和诱导的全身炎症进行时相成像量化。同时，ROS 特异性反应可从纳米孔释放出肾脏可清除的荧光团片段，通过纵向尿液分析监测 HIRI 诱导的系统性炎症。此外，还建立了数字化信号输出（近红外-IIb 比率、尿液荧光）与肝损伤评分之间的功能关系，实现了对 HIRI 严重程度的精确预测和疗效的预先评估。这种分时模块化工具箱可以动态报告 HIRI 引起的体内全身炎症，为 HIRI 治疗提供了一种有效的方法。

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引用次数: 0

Analyte-Induced Specific Regulation of Light-Responsive COF-Cu Nanozyme Activity for Ultrafast Thiram Colorimetric Sensing. 分析物诱导光响应 COF-Cu 纳米酶活性的特异性调节，用于超快 Thiram 色度传感。

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-19 Epub Date: 2024-11-04 DOI: 10.1021/acs.analchem.4c04534

Ling Liang, Ruitao Yang, Jia Wu, Yuan Qin, Yuting Jiang, Shulin Zhao, Fanggui Ye

A light-responsive covalent-organic framework (COF) nanozyme, which integrates the advantages of the COF structure and light-stimulated nanozyme catalysis, is a class of sensing star materials with wide application prospects. However, the sensing methods based on light-responsive COF nanozymes are relatively single at present. Therefore, it is necessary to develop new sensing strategies to broaden its application in chemical sensing and achieve highly efficient detection. Here, a Cu²⁺-modified COF composite material (TpDA-Cu) was rationally designed. The addition of Cu significantly inhibits the excellent light-responsive nanozyme activity of TpDA itself. However, because of the restoration of the enzyme activity by thiram (Tr) and the oxidase mimic activity of the newly formed Cu/Tr complex, TpDA-Cu/Tr exhibits stronger light-responsive nanozyme activity. Enzyme kinetic data show that compared with TpDA, TpDA-Cu/Tr has a larger V_max value, which can achieve efficient catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). In addition, the strong coordination effect of Tr and TpDA-Cu also plays a key role in achieving ultrafast, sensitive, and selective colorimetric detection of Tr. This work develops a dual activity regulation strategy of light-responsive COF nanozymes based on analyte induction and provides a new perspective for the application of light-responsive COF nanozymes in the field of sensing.

光响应共价有机框架（COF）纳米酶综合了COF结构和光刺激纳米酶催化的优点，是一类具有广泛应用前景的传感明星材料。然而，目前基于光响应 COF 纳米酶的传感方法还比较单一。因此，有必要开发新的传感策略，以拓宽其在化学传感中的应用，实现高效检测。本文合理设计了一种 Cu2+修饰的 COF 复合材料（TpDA-Cu）。Cu 的加入明显抑制了 TpDA 本身优异的光响应纳米酶活性。然而，由于噻喃（Tr）对酶活性的恢复作用以及新形成的 Cu/Tr 复合物的氧化酶模拟活性，TpDA-Cu/Tr 表现出更强的光响应纳米酶活性。酶动力学数据表明，与 TpDA 相比，TpDA-Cu/Tr 具有更大的 Vmax 值，可以实现对 3,3',5,5'-四甲基联苯胺（TMB）的高效催化氧化。此外，Tr 和 TpDA-Cu 的强配位效应在实现 Tr 的超快、灵敏和选择性比色检测中也发挥了关键作用。这项工作发展了基于分析物诱导的光响应 COF 纳米酶的双重活性调控策略，为光响应 COF 纳米酶在传感领域的应用提供了新的视角。

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引用次数: 0

Dual-Modality Accurate Visualization of Drug Synergy Based on Mass Spectrometry and Fluorescence Imaging. 基于质谱和荧光成像的药物协同作用双模式精确可视化。

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry

Pub Date : 2024-11-19 Epub Date: 2024-11-05 DOI: 10.1021/acs.analchem.4c03848

Jinlong Zhang, Yida Zhang, Taihe Han, Shuai Mu, Duolong Di, Xuezhao Shi, Xiaoyan Liu, Haixia Zhang

There is a potential synergistic effect between nonsteroidal anti-inflammatory drugs and hydrogen sulfide (H₂S), but direct evidence for the study is lacking. With a single fluorescence detection method, it is difficult to accurately confirm the effectiveness of the synergistic effect. In this study, the fluorescent probe and the nonsteroidal anti-inflammatory drug naproxen were combined via different self-immolative spacer groups to obtain a diagnostic and therapeutic integrated fluorescent probe Nap-NP-NSB, which can release H₂S. The quantitative release of H₂S by Nap-NP-NSB was evaluated in vitro and in cells, and the synergistic effect of H₂S and naproxen was confirmed by monitoring the treatment process of cellular inflammation and oxidative damage of gastric mucosa cells. Finally, in vivo fluorescence imaging and mass spectrometry imaging of the liver and stomach tissues and their sections were performed in the mouse model of acute hepatitis. The dual-modal detection method not only confirmed that Nap-NP-NSB had better anti-inflammatory activity and less gastric mucosal damage, but also enabled a more accurate visualization of the drug synergistic effect of naproxen and H₂S. This work provides a dual visualization imaging method combining fluorescence and mass spectrometry imaging and develops a new idea for studying drug synergies based on self-immolative structures.

非甾体类消炎药与硫化氢（H2S）之间可能存在协同效应，但缺乏直接的研究证据。使用单一的荧光检测方法很难准确确认协同效应的有效性。在这项研究中，荧光探针和非甾体抗炎药萘普生通过不同的自巯基间隔基团结合在一起，得到了一种可释放 H2S 的诊断和治疗一体化荧光探针 Nap-NP-NSB。通过监测细胞炎症和胃黏膜细胞氧化损伤的治疗过程，证实了 H2S 和萘普生的协同作用。最后，在急性肝炎小鼠模型中对肝脏和胃组织及其切片进行了活体荧光成像和质谱成像。双模态检测方法不仅证实了萘普生-萘磺酸具有更好的抗炎活性和更小的胃黏膜损伤，还能更准确地观察到萘普生和 H2S 的药物协同效应。这项工作提供了一种结合荧光成像和质谱成像的双重可视化成像方法，为研究基于自惰性结构的药物协同作用开拓了新思路。

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引用次数: 0