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Production, Optimization and Partial Characterization of Alkaline Protease from Bacillus subtilis spp. subtilis NRRL B-3384 and B-3387 枯草芽孢杆菌B-3384和B-3387碱性蛋白酶的制备、优化及部分特性研究
Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000300
Cengiz Akkale
Bacillus subtilis has been a reliable platform for the expression of extracellular proteases for several decades. Although a majority of Bacillus subtilis subspecies express proteases, the amount of secreted enzyme varies depending on the strain and environmental conditions used. Here, two Bacillus subtilis spp. subtilis strains, NRRL B-3384 and NRRL B-3387, from the ARS Culture collection (NRRL), were compared for secreted protease activity. The highest activity was found in strain NRRL B-3384, and proteolysis occurred at temperatures as high as 80°C and across a broad range of pH, with maximum activity at pH 9.0 and 60°C indicating the presence of a thermostable alkaline protease. To our knowledge, this is the first study to evaluate protease production in Bacillus subtilis spp. subtilis strains NRRL B-3384 and B3387 and suggests that NRRL B-3384 may have utility in the production of enzymes for industrial use.
几十年来,枯草芽孢杆菌一直是细胞外蛋白酶表达的可靠平台。虽然大多数枯草芽孢杆菌亚种表达蛋白酶,但分泌酶的量因菌株和使用的环境条件而异。本研究比较了ARS Culture collection (NRRL)中2株枯草芽孢杆菌(Bacillus subtilis spp. subtilis) NRRL B-3384和NRRL B-3387的蛋白酶活性。菌株NRRL B-3384的酶解活性最高,酶解温度可达80℃,pH范围广,在pH 9.0和60℃时酶解活性最高,表明该菌株为耐热碱性蛋白酶。据我们所知,这是第一次对枯草芽孢杆菌菌株NRRL B-3384和B3387的蛋白酶生产进行评估,并表明NRRL B-3384可能在工业酶生产中具有实用性。
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引用次数: 0
UV and UV+ Water Effects on Mechanical, Thermal and Morphological Properties of Thermoplastic Polyurethane Based Composites UV和UV+水对热塑性聚氨酯基复合材料力学、热学和形态性能的影响
Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000305
S. Sismanoglu
The degradation times of composite materials containing petroleum-based polymer matrix are quite long in nature and it causes environmental pollution. Researchers focus on producing alternative composite materials with organic and/or inorganic fillers to reduce degradation times in nature. In addition, the mechanical and chemical properties of polymer matrix composites are affected by heat, light, moisture and surface compatibility between matrix and filler. In the light of this information, many researchers examine the mechanical, thermal, morphological and similar properties of organic and/or inorganic filler composites by using artificial aging processes using one or more of the water, UV and temperature parameters. In this study, bentonite with surface modification by epoxysilane agent, and bentonite without any surface modification will be added to the thermoplastic polyurethane (TPU) polymer matrix, which has two different segments, and will be mixed in a twin screw extruder and it will then be shaped by the injection molding process. Artificial aging processes of the obtained composites will be done under UV and UV+water for 2 and 4 weeks, respectively. The reason for choosing UV and UV+water processes is that UV and water processes have not been applied together at the same time and there is not much knowledge in the literature on this subject. Mechanical, thermal and morphological properties of composites were investigated.
含石油基聚合物基质的复合材料在自然界中降解时间较长,对环境造成污染。研究人员致力于用有机和/或无机填料生产替代复合材料,以减少自然界中的降解时间。此外,聚合物基复合材料的力学和化学性能受热、光、湿以及基体与填料之间的表面相容性的影响。根据这些信息,许多研究人员通过使用一种或多种水、紫外线和温度参数的人工老化过程来研究有机和/或无机填料复合材料的机械、热、形态和类似性能。在本研究中,将表面经过环氧硅烷改性的膨润土和未经表面改性的膨润土加入到具有两个不同段的热塑性聚氨酯(TPU)聚合物基体中,在双螺杆挤出机中混合,然后进行注射成型工艺。将得到的复合材料分别在紫外线和紫外线+水下进行2周和4周的人工老化处理。选择UV和UV+水工艺的原因是UV和水工艺没有同时应用,文献中关于这一主题的知识不多。研究了复合材料的力学性能、热学性能和形态性能。
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引用次数: 0
Performance Analysis of Fresnel Lens Driven Hot Water/Steam Generator for Domestic and Industrial Use: A CFD Research 菲涅耳透镜驱动家用和工业用热水/蒸汽发生器的性能分析:CFD研究
Pub Date : 2023-03-31 DOI: 10.17350/hjse19030000285
Pinar Mert Cuce, E. Cuce
In this study, the design, manufacture and thermal performance analysis of a Fresnel lens driven hot water/steam generator are presented. The designed system is suitable for domestic and industrial hot water/steam usage and can be easily scaled up to meet different capacity needs. In the first step of the research, thermal behaviour of the cast plate heat exchanger driven by a Fresnel lens with a concentration ratio of 100 is investigated at different working fluid velocities (0.6, 0.8, 1.0, 1.5 and 2.0 m/s) and at different absorber surface temperatures (700, 800, 900 and 1000 °C). Outlet temperature of working fluid from the cast plate heat exchanger is determined through a 3D CFD model for each case. The capacity of the steam generator for different operating times (h = 1, 2 and 3 hours) is also evaluated. The highest working fluid temperature at the outlet of heat exchanger is 914.8 °C for T_cp= 1000 °C and V_wf = 0.6 m/s. On the other hand, the lowest temperature is observed as 424.7 °C for T_cp = 700 °C and V_wf = 2.0 m/s. The steam capacity of the system for h = 3 hours is determined as 1696.5 and 508.9 kg in the best (V_wf = 2.0 m/s) and worst cases (V_wf = 0.6 m/s), respectively.
本文介绍了一种菲涅耳透镜驱动的热水/蒸汽发生器的设计、制造和热性能分析。设计的系统适用于生活和工业热水/蒸汽使用,并且可以很容易地扩大规模以满足不同的容量需求。在研究的第一步,研究了由浓度比为100的菲涅耳透镜驱动的铸板换热器在不同工作流体速度(0.6、0.8、1.0、1.5和2.0 m/s)和不同吸收器表面温度(700、800、900和1000℃)下的热行为。通过三维CFD模型确定了每种情况下铸板换热器工质出口温度。对蒸汽发生器在不同运行时间(h = 1、2和3小时)下的容量进行了计算。当T_cp= 1000℃,V_wf = 0.6 m/s时,换热器出口工作流体最高温度为914.8℃。另一方面,当T_cp = 700℃,V_wf = 2.0 m/s时,最低温度为424.7℃。在最佳工况(V_wf = 2.0 m/s)和最差工况(V_wf = 0.6 m/s)下,系统h = 3 h的蒸汽量分别为1696.5和508.9 kg。
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引用次数: 0
FliA-Dependent Surface Macromolecules Promote Initial Biofilm Development of Escherichia coli by Influencing the Bacterial Surface Properties 依赖于flia的表面大分子通过影响细菌表面特性促进大肠杆菌的初始生物膜发育
Pub Date : 2023-03-13 DOI: 10.17350/hjse19030000295
Fatma Pınar GÖRDESLİ DUATEPE
FliA is an important regulatory component for the synthesis of surface macromolecules which are involved in motility and biofilm development of Escherichia coli. In this study, the roles of FliA-dependent surface macromolecules in E. coli surface tension, surface heterogeneity and surface roughness, and initial biofilm development consisting of reversible and irreversible adhesion were investigated using E. coli MG1655 wild-type strain and fliA gene deleted mutant strain. Negative Gibbs free energy change values calculated using bacterial surface tensions obtained by a spectrophotometric method showed that both wild-type and mutant cells in water can reversibly adhere to the surface of the model solid, silicon nitride (Si3N4). The calculations further showed that bacterial reversible auto-adhesion and co-adhesion were also thermodynamically favorable. In comparison, the reversible adhesion and auto-adhesion capacities of wild-type cells were higher than the mutant cells. Direct measurements by atomic force microscopy (AFM) and thorough analysis of the recorded adhesion data showed that the irreversible adhesion strength of wild-type cells to Si3N4 in water was at least 2.0-fold greater than that of the mutants due to significantly higher surface heterogeneity resulting in higher surface roughness for the wild-type cells compared to those obtained for the mutants. These results suggest that strategies aimed at preventing E. coli biofilm development should also consider a combined method, such as modifying the surface of interest with a bacterial repellent layer and targeting the FliA and FliA-dependent surface macromolecules to reduce both reversible and irreversible bacterial adhesion and hence the initial biofilm development of E. coli.
FliA是大肠杆菌表面大分子合成的重要调控成分,参与大肠杆菌的运动和生物膜发育。本研究以大肠杆菌MG1655野生型菌株和fliA基因缺失突变菌株为研究对象,研究了依赖fliA的表面大分子在大肠杆菌表面张力、表面异质性和表面粗糙度以及由可逆和不可逆粘附组成的初始生物膜发育中的作用。利用分光光度法得到的细菌表面张力计算的负吉布斯自由能变化值表明,野生型和突变型细胞在水中都可以可逆地粘附在模型固体氮化硅(Si3N4)的表面。计算进一步表明,细菌可逆的自粘附和共粘附在热力学上也是有利的。相比之下,野生型细胞的可逆粘附能力和自粘附能力均高于突变型细胞。原子力显微镜(AFM)的直接测量和对记录的粘附数据的深入分析表明,野生型细胞对水中氮化硅的不可逆粘附强度至少是突变体的2.0倍,这是由于与突变体相比,野生型细胞的表面异质性明显更高,导致表面粗糙度更高。这些结果表明,旨在阻止大肠杆菌生物膜发育的策略还应考虑综合方法,例如用细菌排斥层修饰感兴趣的表面,并靶向FliA和依赖FliA的表面大分子,以减少可逆和不可逆的细菌粘附,从而减少大肠杆菌的初始生物膜发育。
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引用次数: 0
Mechanical and wear properties of CoNiAlSiSb and CoNiAlSiIn ferromagnetic shape memory alloys: an experimental assessment CoNiAlSiSb和CoNiAlSiIn铁磁形状记忆合金的机械和磨损性能:实验评估
Pub Date : 2023-02-27 DOI: 10.17350/hjse19030000292
Yusuf Kanca
CoNiAl based ferromagnetic shape memory alloys (FSMAs) are used in various engineering fields, but still need to be improved for tribological applications. In the present study, dry sliding wear behavior of CoNiAlSiSb and CoNiAlSiIn FSMAs was investigated as they were articulated against an alumina abrasive ball using a ball-on-disk tribometer. The experiments were carried out at a load of 20 N, a sliding velocity of 20 mm/s and a sliding distance of 250 m. The worn surfaces were assessed using scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS). The mechanical properties of the CoNiAl based FSMAs were investigated using the nanoindentation technique. The results showed that as compared to CoNiAlSiSb, CoNiAlSiIn FSMA showed a 42% increase in the Young’s modulus and a 10% increase in the microhardness. The mean coefficient of friction (COF) of CoNiAlSiIn (0.56) was observed to be slightly lower than that of CoNiAlSiSb (0.58). The higher hardness and elastic modulus of CoNiAlSiIn than CoNiAlSiIn caused only a 7% increase in the wear resistance. The operative wear mechanisms were abrasion, adhesion and plastic deformation. In conclusion, even though the difference in the tribological performance of the two FSMA surfaces was fairly small, CoNiAlSiIn exhibited better results and thereby would be more preferable.
基于CoNiAl的铁磁形状记忆合金(fsma)广泛应用于各种工程领域,但在摩擦学应用方面仍有待改进。在本研究中,使用球盘式摩擦计研究了CoNiAlSiSb和CoNiAlSiIn fsma在氧化铝磨料球上的干滑动磨损行为。实验在20 N的载荷下进行,滑动速度为20 mm/s,滑动距离为250 m。利用扫描电子显微镜(SEM)和能谱分析(EDS)对磨损表面进行了评估。采用纳米压痕技术对CoNiAl基fsma的力学性能进行了研究。结果表明,与CoNiAlSiSb相比,CoNiAlSiIn FSMA的杨氏模量提高了42%,显微硬度提高了10%。CoNiAlSiIn的平均摩擦系数(COF)为0.56,略低于CoNiAlSiSb(0.58)。conalsiin的硬度和弹性模量均高于conalsiin,但其耐磨性仅提高7%。手术磨损机制为磨损、粘着和塑性变形。综上所述,尽管两种FSMA表面的摩擦学性能差异相当小,但conalsiin表现出更好的结果,因此更可取。
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引用次数: 0
The effect of the Oğuzlar walnut extract as a green corrosion inhibitor on AISI 1010 mild steel Oğuzlar核桃提取物作为绿色缓蚀剂对AISI 1010低碳钢的腐蚀效果
Pub Date : 2023-02-20 DOI: 10.17350/hjse19030000288
M. Okutan, A. Asan, Hacer Ebru Si̇nger
Mild steel is primarily used in reinforced concrete structures, because it has a low corrosion rate due to the formation of a passive oxide film in the alkaline environment. However, when exposed to acidic and atmospheric environments or aqueous environments containing dissolved salts such as sea water, the protective film deteriorates and corrosion occurs due to contamination caused by chloride and carbonation. It is possible to obtain corrosion inhibitors by extraction of bioactive compounds from plants. Thus, inexpensive and environmentally friendly new effective inhibitors are obtained as an alternative to environmentally harmful inhibitors. In this context, the subject of the study was determined as the investigation of the inhibition efficiency of the vanillin phenolic compound in the Çorum Oğuzlar walnut shell extract, which is a local product, on the corrosion of AISI 1010 mild steel in acidic, alkaline and salty environments. The walnut shells, which were cleaned, ground and pre-sieved with a certain grain size, were extracted in seven different solvents. In order to find the appropriate solvent, the total phenolic content (TPC) in the extracts was determined by both the Folin Ciocalteu method and the liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) system. Cyclic voltammetry (CV) and Tafel polarization methods were applied to determine the effect of the extract on the electrochemical behavior of AISI 1010 and its corrosion rate. LC-MS/MS analysis showed the presence of compounds containing p electrons, N and O heteroatoms responsible for the corrosion inhibition. The best inhibition effectiveness was obtained with 86.1% acetone-water mixture in acidic environment (0.2 M HCl).
低碳钢主要用于钢筋混凝土结构,因为它在碱性环境中形成被动氧化膜,腐蚀速率低。然而,当暴露于酸性和大气环境或含有溶解盐的水环境(如海水)时,由于氯化物和碳酸化引起的污染,保护膜会恶化并发生腐蚀。通过从植物中提取生物活性化合物来获得缓蚀剂是可能的。因此,获得了廉价和环保的新型有效抑制剂,以替代对环境有害的抑制剂。在此背景下,本研究的主题被确定为考察当地产品Çorum Oğuzlar核桃壳提取物中香兰素酚类化合物对AISI 1010低碳钢在酸性、碱性和咸味环境中的腐蚀抑制效果。这些核桃壳经过清洗、研磨和预先筛选,具有一定的颗粒大小,用七种不同的溶剂提取。为了寻找合适的溶剂,采用Folin Ciocalteu法和液相色谱-质谱/质谱(LC-MS/MS)联用系统对提取物中总酚含量(TPC)进行测定。采用循环伏安法(CV)和Tafel极化法测定了提取物对AISI 1010电化学行为和腐蚀速率的影响。LC-MS/MS分析表明,含有p电子、N和O杂原子的化合物对腐蚀有抑制作用。在酸性环境(0.2 M HCl)中,以86.1%的丙酮-水混合液的抑菌效果最佳。
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引用次数: 0
Determination of Some Heavy Metals in Wheat Flour of Çorum Province, Turkey: An Air Quality Comparison 土耳其Çorum省小麦粉中部分重金属的测定:空气质量比较
Pub Date : 2023-02-18 DOI: 10.17350/hjse19030000290
Esra Ölmez, Ebru Gökmeşe, Ü. Ergun, Faruk Gokmese
Food security is a priority issue for sustainable global development. In rapid industrialization areas with high fallout of airborne particles, metal uptake by plants could strongly impact crop quality. In this study, concentrations of some heavy metals (Cu, Zn, and Pb) in flour samples supplied in Çorum, defined as one of the “New Industry Focus”, were investigated using inductively coupled plasma-optical emission spectrometry (ICP-OES) to determine the heavy metal contamination. The results showed that the concentration of Pb in all samples examined exceeded the maximum permissible limit. To monitor the increase in Pb concentration and its relationship with air pollution, a two-year laboratory experiment was conducted. It was found that the increase in Pb concentration of about 47% and 77% for two flour samples was consistent with the increase in annual average PM10 concentrations (55% and 82%) obtained from two stations.
粮食安全是全球可持续发展的优先问题。在快速工业化地区,空气中颗粒沉降量高,植物对金属的吸收会严重影响作物品质。本研究利用电感耦合等离子体发射光谱法(ICP-OES)测定了“新产业焦点”之一Çorum供应的面粉样品中重金属(Cu、Zn、Pb)的浓度。结果表明,所有检测样品的铅浓度均超过最大允许限量。为了监测铅浓度的增加及其与空气污染的关系,进行了为期两年的室内试验。结果发现,两种面粉样品的铅浓度分别增加了47%和77%,与两个站点的PM10年平均浓度分别增加了55%和82%一致。
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引用次数: 0
Comparison of R134a and R516A’s performance at Different Air Velocities in Two Evaporator Ejector Cooling System R134a和R516A在两种蒸发器喷射冷却系统中不同风速下的性能比较
Pub Date : 2023-02-16 DOI: 10.17350/hjse19030000293
Ümit Işkan, M. Kahraman, M. Di̇rek
This study aims experimentally to investigate the performance analysis of R134a and alternative R516A refrigerant in two evaporator ejector cooling system (DEES) at different air velocities of evaporator#1. Firstly, the tests were carried out with R134a refrigerant under steady-state conditions at different air velocities and then repeated with low GWP R516A refrigerant. As the tests were carried out with R134a, higher cooling capacity was achieved at different air velocity values. When the air velocity value was 1.1, 1.7, 2.2, and 2.7 ms-1, the COP value obtained from the tests with R134a was 1%, 2%, 5%, and 4% higher than R516A, respectively. Additionally, test results illustrate that the higher air velocity contributed to increasing performance parameters however higher air velocity than 2.2 ms-1 had a slight effect. Based on this study, it is concluded that R516A performance is convenient to R134a performance and can be alternatively used as a refrigerant in vapor compression refrigeration systems(BSS).
实验研究了R134a和R516A替代制冷剂在1号蒸发器喷射冷却系统(DEES)中不同风速下的性能分析。首先用R134a制冷剂在不同风速下进行稳态试验,然后用低GWP值的R516A制冷剂进行重复试验。采用R134a进行试验,在不同的风速值下获得了较高的制冷量。当风速为1.1、1.7、2.2和2.7 ms-1时,R134a的COP值分别比R516A高1%、2%、5%和4%。此外,试验结果表明,较高的风速有助于提高性能参数,但高于2.2 ms-1的风速对性能参数的影响较小。综上所述,R516A性能优于R134a性能,可作为蒸汽压缩制冷系统(BSS)的替代制冷剂。
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引用次数: 0
Influence of past climate changes on the current distribution of a rare and endemic species: Anatolian spiny mouse 过去的气候变化对一种稀有和特有物种:安纳托利亚刺鼠的当前分布的影响
Pub Date : 2023-02-13 DOI: 10.17350/hjse19030000291
Zeycan Helvaci
This research focuses on species distribution modelling (SDM) to have an idea of distribution of Anatolian spiny mouse, Acomys cilicicus in the Last Glacial Maximum (LGM), the mid-Holocene and present by using coordinates along Silifke which is the only location the species found. Three ensembled species distribution models (generalized additive models, maximum entropy and boosted regression trees) were used to project Anatolian spiny mouse environmental suitability. Results indicate that current distribution of A. cilicicus populations shifted east to west since during the Last Glacial Maximum and apparently eastern part of the Mediterranean region of Turkey is potential refugia for this species.
本研究以物种分布模型(SDM)为基础,利用唯一发现地点Silifke的坐标,对Anatolian棘鼠Acomys cilicicus在末次极冰期(Last Glacial Maximum, LGM)、全新世中期及现在的分布情况进行了研究。采用三种集成的物种分布模型(广义加性模型、最大熵模型和增强回归树模型)预测了安纳托利亚棘鼠的环境适应性。结果表明,自末次盛冰期以来,毛蠓种群的分布向东向西转移,土耳其地中海东部地区显然是该物种的潜在避难所。
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引用次数: 0
Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane With the Assistance of polydopamine 聚多巴胺辅助下溶菌酶印迹聚甲基丙烯酸羟乙酯低温凝胶膜的研究
Pub Date : 2023-02-13 DOI: 10.17350/hjse19030000294
A. A. Topcu
The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
在碱性条件下,利用表面印迹技术在聚甲基丙烯酸羟乙酯[p(HEMA)]低温凝胶膜上合成溶菌酶受体。根据人工受体的设计,采用傅里叶变换红外光谱(FTIR)、扫描电镜(SEM)和溶胀实验对其进行了表征研究,并对吸附剂在水溶液中的溶菌酶吸附能力进行了评价。溶菌酶的最大吸附量(Qmax)为108.9 mg/g,吸附剂对目标蛋白的吸附为单层吸附,符合Langmuir等温模型。该吸附剂对肌酐和牛血清白蛋白(BSA)具有较高的选择性。经过5次吸附、解吸和再生循环后,吸附剂仍保持稳定。
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引用次数: 0
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Hittite Journal of Science and Engineering
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