Pub Date : 2023-09-21DOI: 10.21608/avmj.2023.224280.1169
Eman Negm, Alshaimaa Alghriany, Ahmed Mohammed
Cadmium (Cd) is a dangerous environmental pollutant that causes toxicity to humans and animals even when exposed to a small amount as it is hard to expel from the body affecting all body parts. Tribulus terrestris (Tt) is a natural herb used for its medicinal effects around the world. This study directed to detect the possibility of Tt to protect from Cd toxicity in adult male albino rats. Twenty-four rats were separated into four groups, each 6 rats: The first group (C); served as a control without any treatments. Group (2) (Tt + Cd); administrated daily by oral dose of Tt (5 mg/ kg b. wt. liquified in distilled water) for eight weeks then from the start of the ninth week, they daily injected intraperitoneally with Cd (2 mg/kg b. wt.) for eight days. Group (3) (Cd); injected with Cd only at the same dose for eight days. Group (4) (Cd + Tt); firstly, injected with Cd at the same dose for eight days then on the ninth day they had the same Tt dose for eight weeks. In this study, results clarified that Cd application significantly decreased plasma SOD level and the time spent in the opened arms of the elevated plus maze test while increased plasma levels of H 2 O 2 , CRP, IL-6, immunoreaction of brain cleaved caspase-3 and the time spent in the closed arms of the elevated plus maze test compared to control. Administration of Tt succeeded to increase SOD level and the time spent in the opened arms of the elevated plus maze test; however, it reduced plasma levels of H 2 O 2 , CRP, IL-6, immunoreaction of brain cleaved caspase-3 and the time spent in the closed arms of the elevated plus maze test in comparison with Cd group. In conclusion, Tt is highly protective for the brain from harmful Cd effects, referring to its antioxidant, anti-inflammatory and antiapoptotic properties.
{"title":"A Study on the role of Tribulus terrestris in cadmium-induced toxicity on some organs of adult male albino rats","authors":"Eman Negm, Alshaimaa Alghriany, Ahmed Mohammed","doi":"10.21608/avmj.2023.224280.1169","DOIUrl":"https://doi.org/10.21608/avmj.2023.224280.1169","url":null,"abstract":"Cadmium (Cd) is a dangerous environmental pollutant that causes toxicity to humans and animals even when exposed to a small amount as it is hard to expel from the body affecting all body parts. Tribulus terrestris (Tt) is a natural herb used for its medicinal effects around the world. This study directed to detect the possibility of Tt to protect from Cd toxicity in adult male albino rats. Twenty-four rats were separated into four groups, each 6 rats: The first group (C); served as a control without any treatments. Group (2) (Tt + Cd); administrated daily by oral dose of Tt (5 mg/ kg b. wt. liquified in distilled water) for eight weeks then from the start of the ninth week, they daily injected intraperitoneally with Cd (2 mg/kg b. wt.) for eight days. Group (3) (Cd); injected with Cd only at the same dose for eight days. Group (4) (Cd + Tt); firstly, injected with Cd at the same dose for eight days then on the ninth day they had the same Tt dose for eight weeks. In this study, results clarified that Cd application significantly decreased plasma SOD level and the time spent in the opened arms of the elevated plus maze test while increased plasma levels of H 2 O 2 , CRP, IL-6, immunoreaction of brain cleaved caspase-3 and the time spent in the closed arms of the elevated plus maze test compared to control. Administration of Tt succeeded to increase SOD level and the time spent in the opened arms of the elevated plus maze test; however, it reduced plasma levels of H 2 O 2 , CRP, IL-6, immunoreaction of brain cleaved caspase-3 and the time spent in the closed arms of the elevated plus maze test in comparison with Cd group. In conclusion, Tt is highly protective for the brain from harmful Cd effects, referring to its antioxidant, anti-inflammatory and antiapoptotic properties.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136237928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-21DOI: 10.21608/avmj.2023.221332.1164
HEBA NAFADI, AHMED AHMED, RAGAA RASHWAN, SHEREEN GALAL, DOAA ABD EL-KAREEM, RAWHIA ABDEL HAMID
Enterococcus species are Gram-positive cocci that are characterized by being catalase-negative, facultative anaerobic bacteria, and non-spore forming. Enteroccoi is often isolated from environmental and animal sources and inhabits the human gastrointestinal tract. Enterococcus species, which gets its resources from the dairy industry, animals, and people, is one of the most abundant lactic acid bacteria in raw milk. The aim of the present study is to detect antibiotic resistance of Enterococci isolated from raw milk by phenotypic and genotypic methods. Vitek 2 Compact System was used to identify the samples and assess their antimicrobial susceptibility. Following that, drug resistance genes ( ermB , aph (3')-IIIa , and TetM ) and one virulence gene ( esp ) were molecularly detected by PCR. Twenty isolates of Enterococci were phenotypically identified by routine laboratory examination and Vitek2. High rates of antibiotic resistance were found to erythromycin and tetracycline with percentages of 65% and 35%, respectively. The presence of tetM and ermB in milk isolates was found to be 100%, similarly. No detection of aph (3′)IIIa was found in milk isolates. Esp was detected only in 5% of isolated samples. The prevalence of Enterococci among studied samples was 11.8% (20/170). The fact that Enterococci were resistant to erythromycin and tetracycline in our study was noteworthy since these bacteria could potentially be transmitted to people by drinking milk that has not been properly treated.
{"title":"MOLECULAR CHARACTERIZATION OF ANTIBIOTICS RESISTANCE GENES OF ENTEROCOCCI ISOLATED FROM RAW MILK IN ASSIUT CITY","authors":"HEBA NAFADI, AHMED AHMED, RAGAA RASHWAN, SHEREEN GALAL, DOAA ABD EL-KAREEM, RAWHIA ABDEL HAMID","doi":"10.21608/avmj.2023.221332.1164","DOIUrl":"https://doi.org/10.21608/avmj.2023.221332.1164","url":null,"abstract":"Enterococcus species are Gram-positive cocci that are characterized by being catalase-negative, facultative anaerobic bacteria, and non-spore forming. Enteroccoi is often isolated from environmental and animal sources and inhabits the human gastrointestinal tract. Enterococcus species, which gets its resources from the dairy industry, animals, and people, is one of the most abundant lactic acid bacteria in raw milk. The aim of the present study is to detect antibiotic resistance of Enterococci isolated from raw milk by phenotypic and genotypic methods. Vitek 2 Compact System was used to identify the samples and assess their antimicrobial susceptibility. Following that, drug resistance genes ( ermB , aph (3')-IIIa , and TetM ) and one virulence gene ( esp ) were molecularly detected by PCR. Twenty isolates of Enterococci were phenotypically identified by routine laboratory examination and Vitek2. High rates of antibiotic resistance were found to erythromycin and tetracycline with percentages of 65% and 35%, respectively. The presence of tetM and ermB in milk isolates was found to be 100%, similarly. No detection of aph (3′)IIIa was found in milk isolates. Esp was detected only in 5% of isolated samples. The prevalence of Enterococci among studied samples was 11.8% (20/170). The fact that Enterococci were resistant to erythromycin and tetracycline in our study was noteworthy since these bacteria could potentially be transmitted to people by drinking milk that has not been properly treated.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136237931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-21DOI: 10.21608/avmj.2023.215900.1152
Asmaa Abdelghany, Mohamed Mohamed, Doaa Abd El-Aziz, Hussein Ahmed
{"title":"DETERMINATION OF SOME MINERALS IN LIVER, GIZZARD AND MEAT OF CHICKEN","authors":"Asmaa Abdelghany, Mohamed Mohamed, Doaa Abd El-Aziz, Hussein Ahmed","doi":"10.21608/avmj.2023.215900.1152","DOIUrl":"https://doi.org/10.21608/avmj.2023.215900.1152","url":null,"abstract":"","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136237932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.21608/avmj.2023.227596.1174
ZAINAB AHMED YOUSSEF, MOHAMMED ABDEL-BAKY, GHADA MURID, FATMA ABDEL-HAMID, FATMA MAHMOUD
Bovine viral diarrhea (BVD) is a common viral disease that can affect both domesticated and wild animals. Despite their economic losses, there isn't much information available on BVD in Assiut Governorate. Therefore, the present study's objectives were to determine the clinical findings, risk factors associations with the infection rate, and molecular diagnosis of BVD virus (BVDV). The current study was conducted on 50 cattle that belonged to different villages in Assiut Governorate, Egypt. The clinical examination findings revealed fever, oral lesions, diarrhea, respiratory symptoms, and corneal opacity. Serum samples were collected for laboratory analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) assay had been employed for BVDV diagnosis. BVDV RNA was found in the serum of seven cattle. There was no significant difference (P<0.05) between the percentages of BVD infection and the sex, age, and breed (native and mixed breed) of molecularly tested cattle. According to the climatologic circumstances of Assiut governorate, there was a discernible variation (P<0.05) between the BVD infection rate and the cold and hot months. It is advisable to emphasize how crucial it is to implement efficient preventative and control measures throughout Egypt in order to reduce the prevalence of BVDV.
{"title":"SOME STUDIES ON BOVINE VIRAL DIARRHEA IN ASSIUT GOVERNORATE, EGYPT","authors":"ZAINAB AHMED YOUSSEF, MOHAMMED ABDEL-BAKY, GHADA MURID, FATMA ABDEL-HAMID, FATMA MAHMOUD","doi":"10.21608/avmj.2023.227596.1174","DOIUrl":"https://doi.org/10.21608/avmj.2023.227596.1174","url":null,"abstract":"Bovine viral diarrhea (BVD) is a common viral disease that can affect both domesticated and wild animals. Despite their economic losses, there isn't much information available on BVD in Assiut Governorate. Therefore, the present study's objectives were to determine the clinical findings, risk factors associations with the infection rate, and molecular diagnosis of BVD virus (BVDV). The current study was conducted on 50 cattle that belonged to different villages in Assiut Governorate, Egypt. The clinical examination findings revealed fever, oral lesions, diarrhea, respiratory symptoms, and corneal opacity. Serum samples were collected for laboratory analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) assay had been employed for BVDV diagnosis. BVDV RNA was found in the serum of seven cattle. There was no significant difference (P<0.05) between the percentages of BVD infection and the sex, age, and breed (native and mixed breed) of molecularly tested cattle. According to the climatologic circumstances of Assiut governorate, there was a discernible variation (P<0.05) between the BVD infection rate and the cold and hot months. It is advisable to emphasize how crucial it is to implement efficient preventative and control measures throughout Egypt in order to reduce the prevalence of BVDV.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135109068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.21608/avmj.2023.204234.1141
Mohammed Nabil, Hussein Ahmed, Doaa abdel-Azez, Fatma ElZahraa Mustafa
{"title":"QUALITY EVALUATION AND PUBLIC HEALTH IMPORTANCE OF PROCESSED CHICKEN MEAT PRODUCTS in Assiut city","authors":"Mohammed Nabil, Hussein Ahmed, Doaa abdel-Azez, Fatma ElZahraa Mustafa","doi":"10.21608/avmj.2023.204234.1141","DOIUrl":"https://doi.org/10.21608/avmj.2023.204234.1141","url":null,"abstract":"","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"2010 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135109069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-19DOI: 10.21608/avmj.2023.230936.1180
Nagah Saad
{"title":"The Anti-Helicobacter pylori activity of curcumin during the manufacture and storage of laboratory-prepared ice cream","authors":"Nagah Saad","doi":"10.21608/avmj.2023.230936.1180","DOIUrl":"https://doi.org/10.21608/avmj.2023.230936.1180","url":null,"abstract":"","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135109067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.21608/avmj.2023.227860.1175
MARWA ABDALLAH
Starch is commonly used at a rate of not more than 5% in the formulation of luncheon sausage, which is needed for emulsion stabilization. However, many manufacturers increased their rate of addition as a cost-cutting measure, which is considered a means of adulteration. As a result, this study aimed to develop a reliable, fast, and accurate test for determining starch content in luncheon sausage, as well as to compare the results of this test with those of chemical analysis in order to determine its validity. To accomplish this goal, 2 mm thin slices of emulsion sausages containing 5, 10, 15, 20, and 25% starch were sprayed with a standard iodine solution. The time required for the blue color to fully disappear was calculated, the color intensity was photographed, and a color plate was developed. One thousand emulsion-type luncheon sausage samples belonging to different processing plants were examined for their starch content using both the developed color sheet and the chemical method. The produced sheet's results for > 25% and <5% starch were identical to those of the chemical analysis. However, findings for samples containing 5 to 25% starch showed a slightly lower accuracy (91-96%), but thankfully, the fading time provides a valuable method for determining the starch content. Therefore, the iodine test and the developed calibrated color sheet appeared to be an accurate, rapid, and reliable test for the quantification of starch in luncheon sausage.
{"title":"VALIDATION OF A MODIFIED STARCH-IODINE TEST FOR RAPID DETERMINATION OF STARCH CONTENT IN EMULSION LUNCHEON SAUSAGE","authors":"MARWA ABDALLAH","doi":"10.21608/avmj.2023.227860.1175","DOIUrl":"https://doi.org/10.21608/avmj.2023.227860.1175","url":null,"abstract":"Starch is commonly used at a rate of not more than 5% in the formulation of luncheon sausage, which is needed for emulsion stabilization. However, many manufacturers increased their rate of addition as a cost-cutting measure, which is considered a means of adulteration. As a result, this study aimed to develop a reliable, fast, and accurate test for determining starch content in luncheon sausage, as well as to compare the results of this test with those of chemical analysis in order to determine its validity. To accomplish this goal, 2 mm thin slices of emulsion sausages containing 5, 10, 15, 20, and 25% starch were sprayed with a standard iodine solution. The time required for the blue color to fully disappear was calculated, the color intensity was photographed, and a color plate was developed. One thousand emulsion-type luncheon sausage samples belonging to different processing plants were examined for their starch content using both the developed color sheet and the chemical method. The produced sheet's results for > 25% and <5% starch were identical to those of the chemical analysis. However, findings for samples containing 5 to 25% starch showed a slightly lower accuracy (91-96%), but thankfully, the fading time provides a valuable method for determining the starch content. Therefore, the iodine test and the developed calibrated color sheet appeared to be an accurate, rapid, and reliable test for the quantification of starch in luncheon sausage.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135209653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.21608/avmj.2023.186702.1122
Ahmed Abd Elgaber, Ahmed Mohammed, Usama Mahmoud, Madeha Darwish
The purpose of the research was to determine how stocking density affected the behaviour, growth, physiological responses, and leg condition of broilers. 195 five-day old, unsexed Ross strain chicks were randomly allotted into 3 treatments. low stocking density (10 birds/m 2 , LSD), medium stocking density (15 birds/m 2 , MSD) and high stocking density (18 birds/m 2 , HSD). Each treatment had 3 pens (1.5 m 2 ), and approximately four weeks were spent on the study. The HSD demonstrated reduced rates ( P < 0.05) of laying, locomotion, eating, preening and longer periods of tonic immobility. Final body weight and total body weight gain were significantly ( P < 0.05) reduced in the HSD. Also, HSD group showed increased ( P < 0.05) heterophil/lymphocyte ratio, serum corticosterone, glucose and cholesterol, and significantly increased gait issues and footpad and hock burns. However, no significant (P > 0.05) difference was found in litter quality (moisture, ASH, pH) and bone quality (tibiae and femurs measurements) between all treatments. Conclusion, the results indicate that HSD had a negative effect on broiler ’ s behaviour and welfare indicators, therefore it should be avoided in poultry farms and further investigations are still required to figure out the best methods for its control.
{"title":"Effect of stocking density on broilers behaviour and welfare indices.","authors":"Ahmed Abd Elgaber, Ahmed Mohammed, Usama Mahmoud, Madeha Darwish","doi":"10.21608/avmj.2023.186702.1122","DOIUrl":"https://doi.org/10.21608/avmj.2023.186702.1122","url":null,"abstract":"The purpose of the research was to determine how stocking density affected the behaviour, growth, physiological responses, and leg condition of broilers. 195 five-day old, unsexed Ross strain chicks were randomly allotted into 3 treatments. low stocking density (10 birds/m 2 , LSD), medium stocking density (15 birds/m 2 , MSD) and high stocking density (18 birds/m 2 , HSD). Each treatment had 3 pens (1.5 m 2 ), and approximately four weeks were spent on the study. The HSD demonstrated reduced rates ( P < 0.05) of laying, locomotion, eating, preening and longer periods of tonic immobility. Final body weight and total body weight gain were significantly ( P < 0.05) reduced in the HSD. Also, HSD group showed increased ( P < 0.05) heterophil/lymphocyte ratio, serum corticosterone, glucose and cholesterol, and significantly increased gait issues and footpad and hock burns. However, no significant (P > 0.05) difference was found in litter quality (moisture, ASH, pH) and bone quality (tibiae and femurs measurements) between all treatments. Conclusion, the results indicate that HSD had a negative effect on broiler ’ s behaviour and welfare indicators, therefore it should be avoided in poultry farms and further investigations are still required to figure out the best methods for its control.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135209654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.21608/avmj.2023.196707.1127
Shereen Zakaria Hussein, A. Ibrahim, Abdelnaby M. Elshahawy, K. Hassanein, M. Semieka
The present study was conducted on 9 non-medicated, clinically healthy, adult mongrel male dogs. The dogs had no orthopedic abnormalities. Dogs were subjected to mid-diaphyseal circular bone defect (0.8 cm in diameter) in the left radius bones under general anesthesia. Dogs were divided randomly to be allocated into two groups, each of 3 dogs. The treated group (group T, n = 3), in which bone defects were implanted with the titanium oxide/graphene oxide/chitosan nanocomposite. The control group (group C, n = 3), in which bone defects were allowed for spontaneous healing. Dogs were subjected to clinical and radiographical evaluation 30 days postoperatively. All surgical procedures were conducted under the effect of total intravenous anesthesia (TIVA). Digital cranio-palmar and lateral views were taken for the operated limbs. Cortical defects and depth of the bone defects were recorded using the RadiAnt DICOM viewer version 1.1.2022 software. There was a significant decrease ( P < 0.05 ) in the cortical defect in the treated group compared with the control groups 30 days postoperatively. The treated group recorded a significant decrease ( P < 0.05 ) in 30 days compared to the baseline value. The depth of the bone defects decreased significantly ( P < 0.05 ) in the treated group compared with the untreated group 30 days post-induction of the bone defects. There was a significant decrease ( P < 0.05 ) in the treated group on 30 days compared to the baseline value. The titanium oxide/graphene oxide/chitosan nanocomposite accelerates the healing of bone defects.
{"title":"Nanomaterials implantation for accelerating bone healing","authors":"Shereen Zakaria Hussein, A. Ibrahim, Abdelnaby M. Elshahawy, K. Hassanein, M. Semieka","doi":"10.21608/avmj.2023.196707.1127","DOIUrl":"https://doi.org/10.21608/avmj.2023.196707.1127","url":null,"abstract":"The present study was conducted on 9 non-medicated, clinically healthy, adult mongrel male dogs. The dogs had no orthopedic abnormalities. Dogs were subjected to mid-diaphyseal circular bone defect (0.8 cm in diameter) in the left radius bones under general anesthesia. Dogs were divided randomly to be allocated into two groups, each of 3 dogs. The treated group (group T, n = 3), in which bone defects were implanted with the titanium oxide/graphene oxide/chitosan nanocomposite. The control group (group C, n = 3), in which bone defects were allowed for spontaneous healing. Dogs were subjected to clinical and radiographical evaluation 30 days postoperatively. All surgical procedures were conducted under the effect of total intravenous anesthesia (TIVA). Digital cranio-palmar and lateral views were taken for the operated limbs. Cortical defects and depth of the bone defects were recorded using the RadiAnt DICOM viewer version 1.1.2022 software. There was a significant decrease ( P < 0.05 ) in the cortical defect in the treated group compared with the control groups 30 days postoperatively. The treated group recorded a significant decrease ( P < 0.05 ) in 30 days compared to the baseline value. The depth of the bone defects decreased significantly ( P < 0.05 ) in the treated group compared with the untreated group 30 days post-induction of the bone defects. There was a significant decrease ( P < 0.05 ) in the treated group on 30 days compared to the baseline value. The titanium oxide/graphene oxide/chitosan nanocomposite accelerates the healing of bone defects.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121272786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.21608/avmj.2023.198658.1130
Khadiga Aboelail, Mahmoud Rushdi, N. Waly, A. Mohamed
The present study was undertaken to evaluate fecal activin-A, in comparison with the currently used calprotectin, as a potential biomarker for early detection of ulcerative colitis (UC). Rats were divided into 4 groups (5 rats each). These included the acute UC group, where rats received 3% dextran sodium sulphate (DSS) for six consecutive days, and the chronic UC group, where rats received 3% DSS for three cycles (each cycle composed of 3 days of treatment and 12 days off). Control groups included animals that received tap water for six days and kept it as a negative control for short-term treatment and those received tap water for 45 days and served as a negative control for long-term treatment. Fecal samples were collected from all animal groups at the end of the study. Activin A and calprotectin levels were measured in collected fecal samples using a commercial ELISA kit for rat Calprotectin and rat Activin-A. The results revealed significant increases in fecal activin-A, and fecal calprotectin in chronic UC and a significant increase in fecal activin-A in acute colitis. It could be concluded that fecal activin-A is a useful biomarker for both acute and chronic UC. However, fecal calprotectin can be used as an indicator for chronic UC.
{"title":"Evaluation of fecal Activin-A as a novel biomarker for early diagnosis of ulcerative colitis using experimental murine animal model.","authors":"Khadiga Aboelail, Mahmoud Rushdi, N. Waly, A. Mohamed","doi":"10.21608/avmj.2023.198658.1130","DOIUrl":"https://doi.org/10.21608/avmj.2023.198658.1130","url":null,"abstract":"The present study was undertaken to evaluate fecal activin-A, in comparison with the currently used calprotectin, as a potential biomarker for early detection of ulcerative colitis (UC). Rats were divided into 4 groups (5 rats each). These included the acute UC group, where rats received 3% dextran sodium sulphate (DSS) for six consecutive days, and the chronic UC group, where rats received 3% DSS for three cycles (each cycle composed of 3 days of treatment and 12 days off). Control groups included animals that received tap water for six days and kept it as a negative control for short-term treatment and those received tap water for 45 days and served as a negative control for long-term treatment. Fecal samples were collected from all animal groups at the end of the study. Activin A and calprotectin levels were measured in collected fecal samples using a commercial ELISA kit for rat Calprotectin and rat Activin-A. The results revealed significant increases in fecal activin-A, and fecal calprotectin in chronic UC and a significant increase in fecal activin-A in acute colitis. It could be concluded that fecal activin-A is a useful biomarker for both acute and chronic UC. However, fecal calprotectin can be used as an indicator for chronic UC.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122467482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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