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Serum Paraoxonase-1 Activity And Metabolic Profile In Ewes With Pregnancy Toxaemia 妊娠毒血症母羊血清对氧磷酶-1活性和代谢谱
Pub Date : 2023-06-25 DOI: 10.21608/avmj.2023.199609.1131
Mohammed M. Mohammed, A. Abdelraheim, N. Waly, H. Elsayed
This research intended to assess the energy metabolism indices (BHBA, NEFA and glucose) and evaluate the diagnostic relevance of oxidative stress indicators oxidant (MDA) and antioxidant (TAC and PON-1 enzyme ) , lipid profile and liver enzymes in pregnancy toxaemia in ewes. The study comprised a total number of 72 late pregnant ewes (14-20 weeks of gestation). Ewes were divided based on the result of clinical and biochemical findings (glucose and BHBA) into three groups; a clinical pregnant toxemic group (CPTG) (n=36), a subclinical PTG (n=20) and a control group (CG) (n=16). Blood samples were collected for each group and a panel of biochemical parameters was measured. Most of the biochemical markers showed notable variations in comparison with the control group (P < 0.05). BHBA, NEFA, MDA, Liver enzymes (ALT, AST and γGT), Triglycerides and VLDL in clinical and subclinical pregnant toxaemic groups showed a significant increase above normal values of control group (P < 0.05). Glucose, TAC, PON-1 enzyme, Cholesterol, HDL, LDL and serum proteins in clinical and subclinical pregnant toxaemic groups showed a significant decrease below normal values of control group (P < 0.05). Based on these findings, we suggest that monitoring changes in the presented energy metabolism indices, oxidant-antioxidant status, lipid profile, liver enzymes and protein assays in late pregnant ewes could be useful in early diagnosis of pregnancy toxaemia. The present study also showed that paraoxonase-1 enzyme (PON1) appears to hold potential as a biomarker for diagnosis of pregnancy toxaemia in ewes.
本研究旨在评估能量代谢指标(BHBA、NEFA和葡萄糖)和氧化应激指标(MDA)和抗氧化剂(TAC和PON-1酶)、血脂和肝酶在母羊妊娠毒血症中的诊断意义。该研究共包括72只孕晚期母羊(妊娠14-20周)。根据临床和生化结果(葡萄糖和BHBA)将母羊分为三组;临床妊娠毒血症组(CPTG) (n=36)、亚临床妊娠毒血症组(n=20)和对照组(CG) (n=16)。每组采集血液样本,并测量一组生化参数。大部分生化指标与对照组相比有显著差异(P < 0.05)。临床和亚临床妊娠毒血症组BHBA、NEFA、MDA、肝酶(ALT、AST、γ - gt)、甘油三酯、VLDL均显著高于对照组正常值(P < 0.05)。临床及亚临床妊娠毒血症组葡萄糖、TAC、PON-1酶、胆固醇、HDL、LDL及血清蛋白均低于对照组正常值(P < 0.05)。基于这些发现,我们建议监测妊娠后期母羊的能量代谢指标、氧化-抗氧化状态、脂质谱、肝酶和蛋白质检测的变化可能有助于妊娠毒血症的早期诊断。本研究还表明,对氧磷酶-1酶(PON1)似乎具有作为诊断母羊妊娠毒血症的生物标志物的潜力。
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引用次数: 0
Prevalence of helminthes of sheep in Assiut Governorate, Egypt 埃及阿西尤特省绵羊寄生虫流行情况
Pub Date : 2023-06-24 DOI: 10.21608/avmj.2023.196205.1128
A. Dyab, Sara Mohamed, F. Osman
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引用次数: 0
Evaluation of the hygienic aspects of dried milks and infant formulas 奶粉和婴儿配方奶粉卫生方面的评价
Pub Date : 2023-06-24 DOI: 10.21608/avmj.2023.200471.1134
Omnia El-Tayeb, S. Kamal, M. Sayed
The purpose of the current study was to explore the hygienic aspects of different dried milks and infant formulas procured from different supermarkets and pharmacies in Assiut city, Egypt. All the samples were examined for some sensory, physical, chemical and microbiological parameters. The obtained results for the examined dried milks and infant formulas showed the mean values of the insolubility index as 3.24 & 2.17 mg, respectively; pH as 6.9 & 7.1, respectively. The chemical parameters showed the results of acidity%, moisture content, total solids%, fat% and SNF%. For the results of coliforms, fecal coliforms & E. coli , all the examined samples were acceptable according to the Egyptian Standards. In contrast, B. cereus was detected in 33.33 & 26.67% of the examined dried milk and infant formula samples, respectively. It was noticed that all the samples were free from Cronobacter species . Unfortunately, the unacceptable samples according to Cl. perfringens were 6.67% of the dried milk and 3.33% of the infant formula samples. The average yeasts & molds count was 1.09 × 10 5 and 1.44 × 10 5 , respectively.
本研究的目的是探讨从埃及Assiut市不同超市和药店购买的不同奶粉和婴儿配方奶粉的卫生方面。对所有样品进行了一些感官、物理、化学和微生物参数的检查。奶粉和婴幼儿配方奶粉的不溶性指数平均值分别为3.24和2.17 mg;pH分别为6.9和7.1。化学参数为酸度%、含水量%、总固形物%、脂肪%和SNF%。大肠菌群、粪便大肠菌群和大肠杆菌检测结果均符合埃及标准。干奶和婴幼儿配方奶粉样品中蜡样芽孢杆菌的检出率分别为33.33%和26.67%。所有样品均未检出克罗诺杆菌。不幸的是,根据Cl。在奶粉和婴幼儿配方奶粉样品中,产气荚膜菌分别占6.67%和3.33%。酵母和霉菌的平均数量分别为1.09 × 10 5和1.44 × 10 5。
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引用次数: 1
EVALUATION THE RESISTENCE OF EIMERIA SPP. LOCAL ISOLATES TO ANTICCOCIDIAL DRUGS AND THE EFFICACY OF LIVE ATTENUATED VACCINE AND/OR PREBIOTIC IN CONTROL OF EIMERIA INFECTION IN FAYOUMI CHICKENS 评价当地艾美耳球虫分离株对抗球虫药物的耐药性及减毒活疫苗和/或益生元控制法优米鸡艾美耳球虫感染的效果
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.160748.1089
H. Ahmed, Mahmoud Wardaa, Shehata Mostafa, Arafa Mohsen
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引用次数: 0
HISTOPATHOLOGICAL AND BIOCHEMICAL ASSESSMENT OF LIVER FIBROSIS INDUCED BY CARBON TETRACHLORIDE ADMINISTARTION IN RAT 四氯化碳致大鼠肝纤维化的组织病理学和生化评价
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.181348.1111
E. Shaaban, Nashwa Hamad, Dena Torra, M. Taha
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引用次数: 0
EXPRESSION PROFILE OF TUMOR NECROSIS FACTOR ALPHA DURING SPRING VIREMIA OF CARP VIRUS INFECTION IN NILE TILAPIA 尼罗罗非鱼春季病毒感染时肿瘤坏死因子α的表达谱
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.190678.1121
Nagwa Romeih, E. Abdallah, Mahmoudi Mahmoud, A. Elkamel, A. Fouad
Spring viremia of carp (SVC) is a contagious viral disease that causes high mortality among infected fish. The present study aimed to investigate the expression profile of the tumor necrosis factor alpha (TNF-α) gene following experimental infection of Nile tilapia ( Oreochromis niloticus ) with SVCV. Fish were exposed to SVCV (3.2×10 7 TCID 50 /ml) by immersion for 4 hrs. Then, spleens of both infected and control fish were sampled at various time points (1 h, 12 h, 1 d, 3 d, 5 d, 7 d, 11 d, and 14 d) post-infection (pi). The expression of TNF-α gene at these time points was assessed using reverse transcription quantitative real-time PCR (RT-qPCR). The results revealed a significant upregulation of the TNF-α gene that started from 12 hrs pi and continued to reach its peak at the 3 rd dpi recording a fold change of 2.3 and 6.1, respectively, compared to the control. Subsequently, TNF-α gene expression commenced to regress at 5 th dpi until it became similar to its corresponding control at 11 th and 14 th dpi. To the best of our knowledge, this is the first study exploring one of the immune responses of Nile tilapia after SVCV infection.
鲤春病毒血症(SVC)是一种传染性病毒病,感染后死亡率高。本研究旨在探讨尼罗罗非鱼(Oreochromis niloticus) SVCV感染后肿瘤坏死因子α (TNF-α)基因的表达谱。鱼暴露于SVCV (3.2×10 7 TCID 50 /ml)浸泡4小时。然后分别在感染后1 h、12 h、1 d、3 d、5 d、7 d、11 d和14 d采集感染鱼和对照鱼的脾脏(pi)。采用逆转录实时荧光定量PCR (RT-qPCR)检测各时间点TNF-α基因的表达。结果显示,与对照组相比,TNF-α基因从12小时开始显著上调,并在第3 dpi时继续达到峰值,分别增加2.3倍和6.1倍。随后,TNF-α基因表达在第5 dpi时开始回归,直到第11 dpi和第14 dpi时与相应对照相似。据我们所知,这是第一个探索尼罗罗非鱼在SVCV感染后免疫反应的研究。
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引用次数: 0
EVALUATE THE EFFECT OF SOME PHYTOBIOTICS ON THE CONTROL OF NECROTIC ENTERITIS IN BROILERS CHICKEN 评价几种植物制剂对肉鸡坏死性肠炎的防治效果
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.178952.1107
M. Sayed, Mostafa A. Shahta, Naglaa I. Ali, M. Kotob, U. Mahmoud, Manal B. Mahmoud, O. Amen
The harmful economic impacts on chicken production are caused by the bacterium Clostridium perfringens , which is also responsible for necrotic enteritis in chicken. The study's objective is to assess the beneficial benefits of a few phytobiotics as substitutes for antibiotics when treating necrotic enteritis and broiler performance. Seven treatment groups were created at random from 280 broilers. All groups, except the negative control group, faced C. perfringens type A strain seven days after receiving sporulated coccidia oocyst inoculations. G3 and G4 received a basal diet containing 1 and 2 gm of curcumin /kg of diet, respectively. G5 was given a control infection, while G6 received a basal diet containing 1 and 2 gm of propolis /kg of diet. The results revealed that a high concentration of curcumin 1&2 gm/kg as a feed additive had a significant detrimental effect on chicken growth performance. Moreover, the total count of C. perfringens in the cecum was significantly increased and severe histopathological alterations in the intestinal mucosa, including severe sloughing of villi epithelium were detected. On the other side, Propolis, when added to the diet at a dose of 1-2 gm/kg ration, protected the digestive system and ameliorated the pathological lesions induced by the concurrent infections with Eimeria and C. perfringens. Consequently, a considerable decrease in the overall number of C. perfringens in the cecum, and restoration of the normal histological structure of the intestine and the lesions were mild compared to curcumin groups.
产气荚膜梭状芽胞杆菌(Clostridium perfringens)对鸡肉生产造成了有害的经济影响,这种细菌也会导致鸡的坏死性肠炎。本研究的目的是评估几种替代抗生素治疗坏死性肠炎和肉鸡生产性能的有益效果。从280只肉鸡中随机选出7个处理组。除阴性对照组外,各组在接种孢子球虫卵囊7 d后均面对A型产气荚膜球菌。G3和G4分别饲喂姜黄素添加量为1和2 gm /kg的基础饲粮。G5饲喂对照感染,G6饲喂基础饲粮,基础饲粮中蜂胶含量分别为1和2 gm /kg。结果表明,在饲料中添加1和2 gm/kg的高浓度姜黄素对鸡的生长性能有显著不利影响。盲肠中产气荚膜梭菌总数明显增加,肠黏膜出现严重的组织病理学改变,包括绒毛上皮严重脱落。另一方面,在饲料中添加1 ~ 2 gm/kg的蜂胶,可以保护消化系统,改善艾美耳球虫和产气荚膜荚膜梭菌并发感染引起的病理病变。因此,与姜黄素组相比,盲肠中产气荚膜梭菌总数明显减少,肠道正常组织结构和病变恢复轻微。
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引用次数: 1
THERAPEUTIC POTENTIAL OF SOME ORGANIC ACIDS AND ESSENTIAL OILS AGAINST EIMERIA SPP. ISOLATES COLLECTED FROM BROILER CHICKEN FARMS IN ASSIUT 一些有机酸和精油对来自美国西部肉鸡养殖场的艾美耳球虫分离株的治疗潜力
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.158240.1087
Asmaa AE NASR, G. Sayed, Adel M. Soliman, Gamal SULTAN ZAYED, Shiem El-Sherry
Different Eimeria isolates were collected and identified in 50 intestinal specimens. The therapeutic potential of the organic acids and essential oils blends against E. tenella was compared with the anticoccidial drugs toltrazuril and amprolium in broiler chickens. Results revealed that E. tenella was the most frequent identified Eimeria species (48 %) followed by E. acervulina (30 %) and E. mitis (28 %), while the least detected were E. mivati (2%) and E. brunetti (2 %). Organic acids medication reduced the clinical signs and mortality to 33.3 % compared to amprolium (40 %), Essential oils (40 %), and toltrazuril (53.3 %) medicated groups and the positive control (60 %). The best relative body weight gain percent during the experimental course was achieved by birds medicated with organic acids (40 %) and toltrazuril (40 %) followed by amprolium (30 %) and essential oils (30 %) medicated group and the least in the positive control (10 %). The severity of the cecal lesions was reduced similarly by 34 % in all the medicated groups; all scored 2.3 on average in comparison to 3.5 in the positive control. Both organic acids and essential oils were unable to reduce the output of oocysts by over 17% and 3%, respectively, as opposed to 37% in the toltrazuril and 20.6% in the Amprolium treated groups. Microscopic observations revealed their healing effect of on the cecal tissue (epithelium and inflammatory response). The results support the notion that integrating the organic acids and or essential oils may have the ability to induce positive therapeutic effects similar to some chemical drugs such as toltrazuril and amprolium.
在50份肠道标本中收集并鉴定了不同的艾美耳球虫分离株。比较了有机酸和精油混合制剂与抗球虫药物妥曲祖利和氨苯铵对肉仔鸡柔嫩杆菌的治疗效果。结果表明,大肠tenella最常见的确认了艾美球虫属物种(48%),其次是大肠acervulina(30%)和大肠轻的(28%),而最不被检测出大肠mivati(2%)和大肠brunetti(2%)。与氨苯铵(40%)、精油(40%)、托曲祖利(53.3%)用药组和阳性对照组(60%)相比,有机酸治疗组的临床症状和死亡率降低了33.3%。试验期相对增重率以有机酸组(40%)和托曲祖利组(40%)最高,其次为氨苯铵组(30%)和精油组(30%),阳性对照组最低(10%)。盲肠病变的严重程度在所有药物组中同样降低了34%;所有人的平均得分为2.3分,而阳性对照组的平均得分为3.5分。有机酸和精油都不能分别减少17%和3%以上的卵囊产出,而妥曲祖利组为37%,氨苯铵组为20.6%。显微观察显示其对盲肠组织(上皮和炎症反应)的愈合作用。研究结果支持这样一种观点,即有机酸和/或精油的结合可能有能力产生积极的治疗效果,类似于一些化学药物,如托曲祖利和氨苯丙铵。
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引用次数: 0
SEROPREVALENCE RATE OF COXIELLA BURNETII IN COWS’ SERUM IN ASSIUT GOVERNORATE, EGYPT 埃及assiut省奶牛血清中伯纳氏杆菌的血清流行率
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.189492.1119
Reem M Alsaadawy, Sylvia O. Ahmed, H. Hetta
This study was conducted to determine the seroprevalence of Coxiella burnetii in cows from Assiut Governorate, Egypt. A total of 268 blood samples were collected from cows (176 females and 92 males). The age of these animals ranged between 2 months and more than 3 years. Screened for C. burnetii using indirect enzyme-linked immunosorbent assay. A total of 15.67% (42/268) was serologically positive. The seropositivity was high in females (19.56%) than males (13.63%). The native breed was more infected (17.44%) than Fresian (13.33%) and mixed ones (11.76%). In cows, a higher seroprevalence was observed in the age group more than 1 to 2 years (22.38%). Dairut city recorded the highest seroprevalence rate (40.74%) of C. burnetii . C. burnetii seroprevalence was higher in hot season (20.10%) than cold season (5.95%). Cows free from ticks recorded higher seroprevalence (22.58%) than those infested with ticks (12%). Cows with fever and pneumonia recorded higher seroprevalence (14.29%) compared to animals with other signs. In conclusion, findings of this study revealed the wide spread of C. burnetii infection among cows at Assiut Governorate, Egypt.
本研究旨在确定埃及阿西尤特省奶牛的伯氏科希氏菌血清流行率。从奶牛(176头母牛和92头公牛)共采集了268份血液样本。这些动物的年龄从2个月到3岁多不等。采用间接酶联免疫吸附法筛选伯纳蒂梭菌。血清学阳性率为15.67%(42/268)。血清阳性率女性(19.56%)高于男性(13.63%)。本地品种感染率为17.44%,高于法国品种(13.33%)和混合品种(11.76%)。在奶牛中,1 ~ 2岁以上年龄组血清阳性率较高(22.38%)。乳鲁特市布氏原体血清阳性率最高,为40.74%。热季布氏疏螺旋体血清阳性率(20.10%)高于冷季(5.95%)。无蜱牛血清阳性率(22.58%)高于有蜱牛血清阳性率(12%)。与有其他症状的奶牛相比,有发烧和肺炎的奶牛血清阳性率(14.29%)较高。总之,本研究结果揭示了埃及阿西尤特省奶牛中伯纳蒂胞杆菌感染的广泛传播。
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引用次数: 0
ELISA ASSAYS VERSUS COPROLOGICAL DETECTION OF FASCIOLA HEPATICA IN HORSES IN SULAIMANI PROVINCE-NORTH IRAQ Elisa法测定伊拉克北部苏莱曼尼省马肝片形吸虫的粪学检测
Pub Date : 2023-04-01 DOI: 10.21608/avmj.2023.184656.1114
B. Ali, K. Ali, H. Marif, Othman J. Ali, Shihab Mustafa
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引用次数: 0
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Assiut Veterinary Medical Journal
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