Pub Date : 2023-07-03DOI: 10.21608/avmj.2023.201849.1135
Mina D Fahmy
This study was designed to evaluate the quality of yoghurt sold in Assiut city, Egypt, in which a total of 60 samples of Baladi, pasteurized plain and pasteurized flavored (20 each) were collected randomly. The sensory evaluation was based on visual, texture and flavor. Syneresis was applied for rheological properties and pH for physical properties. Chemical analysis was applied through moisture, TS, fat and SNF%. Also, titratable acidity% and starch were detected. The microbiological examination for coliforms, fecal coliforms, E. coli , anaerobes and yeasts & molds were counted. The achieved results showed that the sensory evaluation of the pasteurized plain and pasteurized flavored samples was of higher scores than the Baladi samples, however on contrast, syneresis was higher in the Baladi samples. The average values of pH were 4.9, 4.89 and 4.83 for the Baladi, pasteurized plain and pasteurized flavored samples, respectively. For the fat content, the Baladi samples had higher fat%, followed by the pasteurized plain then the pasteurized flavored types. All the examined Baladi samples were starch free, while 70 & 65% of the pasteurized plain and pasteurized flavored types were positive, respectively. The average values of titratable acidity% were 0.91, 0.82 and 0.75, respectively. The microbiological examination cleared that the Baladi samples were more contaminated for coliforms, fecal coliforms and E. coli , while, the pasteurized plain and pasteurized flavored samples were more contaminated for anaerobes. It was found that 85% of the total examined samples were unacceptable according to the Egyptian Standards for their content of yeasts and molds.
{"title":"Sensory, rheological, physical, chemical and microbiological properties of different types of yoghurt.","authors":"Mina D Fahmy","doi":"10.21608/avmj.2023.201849.1135","DOIUrl":"https://doi.org/10.21608/avmj.2023.201849.1135","url":null,"abstract":"This study was designed to evaluate the quality of yoghurt sold in Assiut city, Egypt, in which a total of 60 samples of Baladi, pasteurized plain and pasteurized flavored (20 each) were collected randomly. The sensory evaluation was based on visual, texture and flavor. Syneresis was applied for rheological properties and pH for physical properties. Chemical analysis was applied through moisture, TS, fat and SNF%. Also, titratable acidity% and starch were detected. The microbiological examination for coliforms, fecal coliforms, E. coli , anaerobes and yeasts & molds were counted. The achieved results showed that the sensory evaluation of the pasteurized plain and pasteurized flavored samples was of higher scores than the Baladi samples, however on contrast, syneresis was higher in the Baladi samples. The average values of pH were 4.9, 4.89 and 4.83 for the Baladi, pasteurized plain and pasteurized flavored samples, respectively. For the fat content, the Baladi samples had higher fat%, followed by the pasteurized plain then the pasteurized flavored types. All the examined Baladi samples were starch free, while 70 & 65% of the pasteurized plain and pasteurized flavored types were positive, respectively. The average values of titratable acidity% were 0.91, 0.82 and 0.75, respectively. The microbiological examination cleared that the Baladi samples were more contaminated for coliforms, fecal coliforms and E. coli , while, the pasteurized plain and pasteurized flavored samples were more contaminated for anaerobes. It was found that 85% of the total examined samples were unacceptable according to the Egyptian Standards for their content of yeasts and molds.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134088924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.202244.1136
A. Elrawy, A. Mohammed, Mahmoud S. Sabra, U. Mahmoud, M. Darwish
Rumen juice transfaunation (RT) has been suggested as one of the effective ways to improve the performance and health of the host. This study's objective was to investigate the effect of RT on behavior, performance and kidney function of fattening lambs. Twelve male lambs were divided into three groups at random: controls (CON, n = 4): lambs were given one liter of normal saline, (Ts, n = 4): lambs received 1 liter of sheep ruminal juice and (Tc, n = 4): lambs received 1 liter of cattle ruminal juice, once throughout the study. Each lamb was caged in a single pen with alive weight of 20.7 (±1.95) and the experiment lasted six weeks. The instantaneous scan sampling approach was used to record behavioral observations on days 1, 2, 3, 7, 8, 15, 16, 30, 31 and 45 & 46 after rumen fluid transfauntion; production parameters and kidney function were measured every week. Rumen juice transfaunation resulted in a significant decrease in chewing pen fixtures behavior especially in Ts and feed conversion ratio in both (Ts and Tc) at first and second weeks, however a significant increase in body weight in both (Ts and Tc) at third, fifth and sixth week compared to control lambs. There was no significant effect on the serum creatinine and urea levels. In conclusion, the current results indicate that RT has a beneficial effect on fattening lamb’s behavior and performance; therefore it can be used in intensive fattening sheep farms.
瘤胃液转化(RT)已被认为是改善宿主生产性能和健康的有效途径之一。本研究旨在探讨RT对育肥羔羊行为、生产性能和肾功能的影响。将12只雄性羔羊随机分为3组:对照组(CON, n = 4):给予1升生理盐水，对照组(Ts, n = 4):给予1升绵羊瘤胃液，对照组(Tc, n = 4):给予1升牛瘤胃液，在整个研究过程中各给予1升。每只羔羊单圈饲养，活重20.7(±1.95)，试验期6周。采用瞬时扫描采样法记录瘤胃液移植后第1、2、3、7、8、15、16、30、31、45、46天的行为观察;每周检测生产参数及肾功能。瘤胃液移植导致第1周和第2周的咀嚼栏固定行为显著降低，尤其是Ts和Tc的饲料转化率显著降低，而第3、5和6周的体重(Ts和Tc)均显著高于对照羔羊。对血清肌酐和尿素水平无显著影响。综上所述，RT对育肥羔羊的行为和生产性能有有益的影响;因此，它可以用于集约化育肥羊场。
{"title":"IMPACT OF RUMEN JUICE TRANSFAUNATION ON BEHAVIORAL ACTIVITIES, PERFORMANCE PARAMETERS AND KIDNEY FUNCTION IN FATTENING LAMBS","authors":"A. Elrawy, A. Mohammed, Mahmoud S. Sabra, U. Mahmoud, M. Darwish","doi":"10.21608/avmj.2023.202244.1136","DOIUrl":"https://doi.org/10.21608/avmj.2023.202244.1136","url":null,"abstract":"Rumen juice transfaunation (RT) has been suggested as one of the effective ways to improve the performance and health of the host. This study's objective was to investigate the effect of RT on behavior, performance and kidney function of fattening lambs. Twelve male lambs were divided into three groups at random: controls (CON, n = 4): lambs were given one liter of normal saline, (Ts, n = 4): lambs received 1 liter of sheep ruminal juice and (Tc, n = 4): lambs received 1 liter of cattle ruminal juice, once throughout the study. Each lamb was caged in a single pen with alive weight of 20.7 (±1.95) and the experiment lasted six weeks. The instantaneous scan sampling approach was used to record behavioral observations on days 1, 2, 3, 7, 8, 15, 16, 30, 31 and 45 & 46 after rumen fluid transfauntion; production parameters and kidney function were measured every week. Rumen juice transfaunation resulted in a significant decrease in chewing pen fixtures behavior especially in Ts and feed conversion ratio in both (Ts and Tc) at first and second weeks, however a significant increase in body weight in both (Ts and Tc) at third, fifth and sixth week compared to control lambs. There was no significant effect on the serum creatinine and urea levels. In conclusion, the current results indicate that RT has a beneficial effect on fattening lamb’s behavior and performance; therefore it can be used in intensive fattening sheep farms.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115639898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.202372.1137
H. Farghaly, M. Bakr, Hoda E. L. Sayed, I. Ashry
Autism spectrum disorders are a neurodevelopmental condition characterized by disturbances in social interaction and communication, as well as repetitive and restricted behaviors. No efficient treatment plans have been found so far. The neurodevelopmental effects of antiandrogens on developmental milestones were examined in rats following a single intraperitoneal injection of adult pregnant female rats with valproic acid (VPA) (600 mg/kg) on gestation day 12.5. Control females were injected with physiological saline at the same time. The offspring of set 1 (which received physiological saline) was divided into 2 groups (groups 1&2) and the offspring of set 2 (which received VPA) were divided into 5 groups (groups 3-7). Thus, the seven groups were organized as follows: Group 1, the control group in which pups did not receive any treatment. Group 2, pups were given sesame oil vehicle. Group 3 pups did not receive any further treatment. Group 4 in which the pups were given sesame oil. Group 5 in which the pups received 1 mg/day testosterone propionate subcutaneously. Group 6 in which the pups received flutamide 1 mg/kg subcutaneously. Group 7 in which the pups received finasteride 50 mg/kg subcutaneously. Our results demonstrated that VPA-induced retardation in sensorimotor reflexes, but did not affect physical landmarks while antiandrogens improved developmental milestones. In conclusion, our finding confirms that androgen contributes partially towards VPA-induced neurodevelopmental insults.
{"title":"EFFECT OF ANTIANDROGENS ON VALPROIC ACID-INDUCED NEURODEVELOPMENTAL CHANGES IN RATS","authors":"H. Farghaly, M. Bakr, Hoda E. L. Sayed, I. Ashry","doi":"10.21608/avmj.2023.202372.1137","DOIUrl":"https://doi.org/10.21608/avmj.2023.202372.1137","url":null,"abstract":"Autism spectrum disorders are a neurodevelopmental condition characterized by disturbances in social interaction and communication, as well as repetitive and restricted behaviors. No efficient treatment plans have been found so far. The neurodevelopmental effects of antiandrogens on developmental milestones were examined in rats following a single intraperitoneal injection of adult pregnant female rats with valproic acid (VPA) (600 mg/kg) on gestation day 12.5. Control females were injected with physiological saline at the same time. The offspring of set 1 (which received physiological saline) was divided into 2 groups (groups 1&2) and the offspring of set 2 (which received VPA) were divided into 5 groups (groups 3-7). Thus, the seven groups were organized as follows: Group 1, the control group in which pups did not receive any treatment. Group 2, pups were given sesame oil vehicle. Group 3 pups did not receive any further treatment. Group 4 in which the pups were given sesame oil. Group 5 in which the pups received 1 mg/day testosterone propionate subcutaneously. Group 6 in which the pups received flutamide 1 mg/kg subcutaneously. Group 7 in which the pups received finasteride 50 mg/kg subcutaneously. Our results demonstrated that VPA-induced retardation in sensorimotor reflexes, but did not affect physical landmarks while antiandrogens improved developmental milestones. In conclusion, our finding confirms that androgen contributes partially towards VPA-induced neurodevelopmental insults.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128947233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.213997.1148
Faten ABD ELATY, Yasmin O. El-Amir, Mohamed Abdel Rahman, A. Hassan
The goal of the study is to examine the potentially harmful histopathological and biochemical effects of 5-Fluorouracil (5-FU) on the liver and kidney. Thirty rats were divided into two groups. The control group was given physiological saline intraperitoneally for 5 days. The 5-FU group received 5-FU at a dose of 20 mg/kg b. wt. intraperitoneally for 5 days. Blood samples were collected and used for biochemical indexes. For histological analysis, samples of the liver and kidney were collected. There was a significant increase in the levels of serum creatinine, urea, aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA( in the 5-FU group when compared to the control group. Compared to the control group, there was a significant decrease in total antioxidants (TAO). Microscopic examination of the liver showed vascular changes, apoptosis, vacuolar degeneration, kupffer cell proliferation, focal inflammatory cell infiltration, hepatocellular necrosis and mononuclear cellular infiltration in the portal area. While the histopathological changes in kidneys varied from vascular changes, glomerular necrosis and atrophy and vacuolar degeneration of the renal tubules. It was concluded that the hepatotoxic and nephrotoxic effects were due to the oxidative stress induced by 5-FU.
该研究的目的是检查5-氟尿嘧啶(5-FU)对肝脏和肾脏的潜在有害组织病理学和生化影响。30只大鼠被分为两组。对照组小鼠腹腔灌胃生理盐水5 d。5- fu组给予5- fu，剂量为20 mg/kg b. wt，腹腔注射5 d。采集血样进行生化指标测定。为进行组织学分析，收集肝脏和肾脏样本。与对照组相比，5-FU组血清肌酐、尿素、谷草转氨酶(AST)、丙氨酸转氨酶(ALT)、丙二醛(MDA)水平显著升高。与对照组相比，总抗氧化剂(TAO)显著降低。肝脏镜下可见血管改变、细胞凋亡、空泡变性、库普弗细胞增生、局灶性炎症细胞浸润、肝细胞坏死及门静脉区单核细胞浸润。而肾脏的组织病理学改变从血管改变，肾小球坏死和萎缩以及肾小管空泡变性。结论5-FU的肝毒性和肾毒性作用是由氧化应激引起的。
{"title":"HISTOPATHOLOGICAL AND BIOCHEMICAL EVALUATION OF HEPATOTOXICITY AND NEPHROTOXICITY INDUCED BY 5- FLUOROURACIL IN RATS","authors":"Faten ABD ELATY, Yasmin O. El-Amir, Mohamed Abdel Rahman, A. Hassan","doi":"10.21608/avmj.2023.213997.1148","DOIUrl":"https://doi.org/10.21608/avmj.2023.213997.1148","url":null,"abstract":"The goal of the study is to examine the potentially harmful histopathological and biochemical effects of 5-Fluorouracil (5-FU) on the liver and kidney. Thirty rats were divided into two groups. The control group was given physiological saline intraperitoneally for 5 days. The 5-FU group received 5-FU at a dose of 20 mg/kg b. wt. intraperitoneally for 5 days. Blood samples were collected and used for biochemical indexes. For histological analysis, samples of the liver and kidney were collected. There was a significant increase in the levels of serum creatinine, urea, aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA( in the 5-FU group when compared to the control group. Compared to the control group, there was a significant decrease in total antioxidants (TAO). Microscopic examination of the liver showed vascular changes, apoptosis, vacuolar degeneration, kupffer cell proliferation, focal inflammatory cell infiltration, hepatocellular necrosis and mononuclear cellular infiltration in the portal area. While the histopathological changes in kidneys varied from vascular changes, glomerular necrosis and atrophy and vacuolar degeneration of the renal tubules. It was concluded that the hepatotoxic and nephrotoxic effects were due to the oxidative stress induced by 5-FU.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125515744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.205698.1144
Shimaa S Abdelhady, K. Hassanein, M. Taha
Doxorubicin (DOX) is an antitumor drug that causes hepatotoxicity by release of free radicals and injury of the liver tissues. Nano-thymoquinone (nano-TQ) is considered a potent antioxidant that abrogates hepatotoxicity. The aim of the current investigation was to assess the liver toxicity of DOX and the protective effects of nano-TQ on chronic hepatotoxicity in male rats. Sixty rats were divided into four even groups: DOX treated group (group 1) received 3.750 mg/kg b.wt. intraperitoneally at days 10,17,24 and 31 from the beginning of the experiment to make a cumulative dose of 15 mg/kg b.wt., Group (2) received DOX as group 1 beside nano-thymoquinone daily from day one in a dose of 10 mg/kg b.wt. until the end of the experiment, Group (3) received nano-TQ only, group (4) is considered a negative control group. Serum samples were used for estimating oxidative stress markers and liver function enzymes. Liver specimens were used for histopathological and Transmission electron microscopic (TEM) examination. Doxorubicin administration induced an increase in liver enzymes and lipid peroxidation products. Nano-TQ treatment improved those altered parameters. Microscopic examination of the DOX-treated liver sections revealed vascular and parenchymatous alterations as congestion, thrombosis of the blood vessels, vacuolar degeneration, hepatocellular necrosis and fibrosis of the liver. While nano-TQ improved such pathological alteration of the hepatic parenchyma. Transmission electron microscope of the liver revealed infiltration of lymphocyte in the necrotic areas and presence of fat globules in the cytoplasm of the hepatocytes in DOX treated group while TEM revealed a unique finding in DOX and nano-TQ treated group expressed by hypertrophy of Kupffer cells. It could be concluded that nano-TQ has a potent antioxidant effect that protected the liver damage via its free radicals scavenging protection.
{"title":"THYMOQUINONE NANOTHERAPY ABROGATES HEPATOTOXICITY-INDUCED BY DOXORUBICIN IN MALE ALBINO RATS","authors":"Shimaa S Abdelhady, K. Hassanein, M. Taha","doi":"10.21608/avmj.2023.205698.1144","DOIUrl":"https://doi.org/10.21608/avmj.2023.205698.1144","url":null,"abstract":"Doxorubicin (DOX) is an antitumor drug that causes hepatotoxicity by release of free radicals and injury of the liver tissues. Nano-thymoquinone (nano-TQ) is considered a potent antioxidant that abrogates hepatotoxicity. The aim of the current investigation was to assess the liver toxicity of DOX and the protective effects of nano-TQ on chronic hepatotoxicity in male rats. Sixty rats were divided into four even groups: DOX treated group (group 1) received 3.750 mg/kg b.wt. intraperitoneally at days 10,17,24 and 31 from the beginning of the experiment to make a cumulative dose of 15 mg/kg b.wt., Group (2) received DOX as group 1 beside nano-thymoquinone daily from day one in a dose of 10 mg/kg b.wt. until the end of the experiment, Group (3) received nano-TQ only, group (4) is considered a negative control group. Serum samples were used for estimating oxidative stress markers and liver function enzymes. Liver specimens were used for histopathological and Transmission electron microscopic (TEM) examination. Doxorubicin administration induced an increase in liver enzymes and lipid peroxidation products. Nano-TQ treatment improved those altered parameters. Microscopic examination of the DOX-treated liver sections revealed vascular and parenchymatous alterations as congestion, thrombosis of the blood vessels, vacuolar degeneration, hepatocellular necrosis and fibrosis of the liver. While nano-TQ improved such pathological alteration of the hepatic parenchyma. Transmission electron microscope of the liver revealed infiltration of lymphocyte in the necrotic areas and presence of fat globules in the cytoplasm of the hepatocytes in DOX treated group while TEM revealed a unique finding in DOX and nano-TQ treated group expressed by hypertrophy of Kupffer cells. It could be concluded that nano-TQ has a potent antioxidant effect that protected the liver damage via its free radicals scavenging protection.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131866815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.186958.1117
H. Shehata, H. Elsayed, N. Waly
In Egypt, dogs are routinely fed a home-cooked diet prepared by their owner (e.g. boiled chicken legs). The nutrition of pets is crucial to the health state of various body systems and that can be reflected in the complete blood count picture as well as general health of the animal. Poor nutrition can result in various nutritional deficiency disorders such as nutritional deficiency anemia. Nine native breed puppies were screened at regular intervals using a general health physical examination including monitoring their temperature, general inspection, heart and respiratory rate measurements before changing their diet in a controlled experiment. The dogs were kept indoors and their diet was changed from the traditional home cooked food into a commercially available dry diet. Blood samples were also collected for a complete blood count on day one before starting the commercial diet and day 30 at the end of the experiment. The complete blood picture was monitored on set days throughout the experiment and the fecal score was assessed using a fecal scoring system of Royal Canine on day 1 before starting the commercial diet and day 30 at the end of the experiment. There was a significant difference in most blood parameters following the change in diet. Those that were significantly higher were: the total RBCs count, hemoglobin concentration, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin and the mean corpuscular hemoglobin concentration (p<0.05). The fecal score also improved from loose stool into clearly defined shape and that change was statistically significant (p<0.05).
{"title":"INVESTIGATING THE EFFECT OF A CANINE COMMERCIAL DRY DIET ON THE COMPLETE BLOOD PICTURE AND FECAL QUALITY IN DOMESTIC DOGS PREVIOUSLY KEPT ON HOME-PREPARED FOOD IN EGYPT","authors":"H. Shehata, H. Elsayed, N. Waly","doi":"10.21608/avmj.2023.186958.1117","DOIUrl":"https://doi.org/10.21608/avmj.2023.186958.1117","url":null,"abstract":"In Egypt, dogs are routinely fed a home-cooked diet prepared by their owner (e.g. boiled chicken legs). The nutrition of pets is crucial to the health state of various body systems and that can be reflected in the complete blood count picture as well as general health of the animal. Poor nutrition can result in various nutritional deficiency disorders such as nutritional deficiency anemia. Nine native breed puppies were screened at regular intervals using a general health physical examination including monitoring their temperature, general inspection, heart and respiratory rate measurements before changing their diet in a controlled experiment. The dogs were kept indoors and their diet was changed from the traditional home cooked food into a commercially available dry diet. Blood samples were also collected for a complete blood count on day one before starting the commercial diet and day 30 at the end of the experiment. The complete blood picture was monitored on set days throughout the experiment and the fecal score was assessed using a fecal scoring system of Royal Canine on day 1 before starting the commercial diet and day 30 at the end of the experiment. There was a significant difference in most blood parameters following the change in diet. Those that were significantly higher were: the total RBCs count, hemoglobin concentration, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin and the mean corpuscular hemoglobin concentration (p<0.05). The fecal score also improved from loose stool into clearly defined shape and that change was statistically significant (p<0.05).","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116534243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.213953.1147
Hayam Z. Thabet, I. Seddik, Wegdan A. Mohamed, A. Sayed, Shaymaa Yusuf
The need for enzymes is growing across a number of industries due to their several advantages, such as their minimal environmental toxicity. Lipases are one of the most valuable biotechnological enzymes. As a result, studies on lipases have become significantly popular in the field of enzymology in recent years. Several attempts have been made to isolate different bacterial isolates that can produce the lipase enzyme. The current study aims to isolate diverse bacterial isolates from different sources: soil contaminated with oil, animal wounds, and contaminated culture media. A qualitative screening for lipase-producing isolates was conducted using tween 80 agar. The results showed that a total of 124 bacterial isolates were obtained from different sources, including 41 isolates that can produce the lipase enzyme. Upon the lipase activity assay, the 20 highest lipase-producing isolates were identified phenotypically. The best potential lipase producers were further identified using 16S rRNA sequencing as Brevibacillus sp. strain HC1 and Brevibacillus sp. strain HS5, with the accession numbers OR048061 and OR048060, respectively. This study highlights the significance of using bacteria as a microbial source for lipase enzyme production for future industrial and biotechnological applications.
{"title":"ISOLATION, SCREENING, AND MOLECULAR IDENTIFICATION OF LIPASE-PRODUCING BACTERIA FROM DIFFERENT BACTERIAL SOURCES","authors":"Hayam Z. Thabet, I. Seddik, Wegdan A. Mohamed, A. Sayed, Shaymaa Yusuf","doi":"10.21608/avmj.2023.213953.1147","DOIUrl":"https://doi.org/10.21608/avmj.2023.213953.1147","url":null,"abstract":"The need for enzymes is growing across a number of industries due to their several advantages, such as their minimal environmental toxicity. Lipases are one of the most valuable biotechnological enzymes. As a result, studies on lipases have become significantly popular in the field of enzymology in recent years. Several attempts have been made to isolate different bacterial isolates that can produce the lipase enzyme. The current study aims to isolate diverse bacterial isolates from different sources: soil contaminated with oil, animal wounds, and contaminated culture media. A qualitative screening for lipase-producing isolates was conducted using tween 80 agar. The results showed that a total of 124 bacterial isolates were obtained from different sources, including 41 isolates that can produce the lipase enzyme. Upon the lipase activity assay, the 20 highest lipase-producing isolates were identified phenotypically. The best potential lipase producers were further identified using 16S rRNA sequencing as Brevibacillus sp. strain HC1 and Brevibacillus sp. strain HS5, with the accession numbers OR048061 and OR048060, respectively. This study highlights the significance of using bacteria as a microbial source for lipase enzyme production for future industrial and biotechnological applications.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"60 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131809420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/avmj.2023.200439.1133
Shimaa Hassanein, W. Elsherif, Mohamed A. Elshater, M. Sayed
The purpose of the current study was to evaluate the degree of contamination of dairy farms with both Pseudomonas aeruginosa and Staphylococcus aureus , in which, a total of 150 swab samples were collected from 3 different dairy farms (50 each) distributed in Assiut governorate (farm A) and El-Minia governorate (farms B & C), Egypt. The swabs were taken from different locations in the dairy farms such as teats of dairy animals, milking machines, workers' hands and stanchions, etc. All the samples were examined for detection of Ps. aeruginosa (conventionally biochemical followed by MICROBACT™ identification kits) and for Staph. aureus (coagulase +ve). The obtained results revealed the overall incidence of Ps. aeruginosa as 14%, 2% and 4% in farms A, B & C, respectively; while, the incidence of Staph. aureus was 4%, 30% and 4%, respectively. In conclusion, the profile of both Ps. aeruginosa and Staph. aureus among the examined dairy farms is highlighting the hygienic state of each farm separately.
{"title":"PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS PROFILE IN SOME DAIRY FARMS","authors":"Shimaa Hassanein, W. Elsherif, Mohamed A. Elshater, M. Sayed","doi":"10.21608/avmj.2023.200439.1133","DOIUrl":"https://doi.org/10.21608/avmj.2023.200439.1133","url":null,"abstract":"The purpose of the current study was to evaluate the degree of contamination of dairy farms with both Pseudomonas aeruginosa and Staphylococcus aureus , in which, a total of 150 swab samples were collected from 3 different dairy farms (50 each) distributed in Assiut governorate (farm A) and El-Minia governorate (farms B & C), Egypt. The swabs were taken from different locations in the dairy farms such as teats of dairy animals, milking machines, workers' hands and stanchions, etc. All the samples were examined for detection of Ps. aeruginosa (conventionally biochemical followed by MICROBACT™ identification kits) and for Staph. aureus (coagulase +ve). The obtained results revealed the overall incidence of Ps. aeruginosa as 14%, 2% and 4% in farms A, B & C, respectively; while, the incidence of Staph. aureus was 4%, 30% and 4%, respectively. In conclusion, the profile of both Ps. aeruginosa and Staph. aureus among the examined dairy farms is highlighting the hygienic state of each farm separately.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128406279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-29DOI: 10.21608/avmj.2023.217186.1154
Mohamed El Asuoty
{"title":"Situation of Aflatoxins residues in chicken and duck meat","authors":"Mohamed El Asuoty","doi":"10.21608/avmj.2023.217186.1154","DOIUrl":"https://doi.org/10.21608/avmj.2023.217186.1154","url":null,"abstract":"","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126260922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.21608/avmj.2023.203699.1140
Ahmed Khayri, H. Hussein, Rehab Sayed, N. Waly
Dogs’ temperature is measured through the rectum, which can be difficult to obtain in animals with aggressive nature, or those with ano-rectal conditions. Alternative instruments and routes have also been developed. These alternative methods may yield different results. This study aimed to compare different methods in measuring the internal temperature of dogs. Cases referred to the small animal clinic (n=20) for a general health check were included in this study upon confirming their health status. Axillary, tympanic membrane and rectal temperature were measured 30 minutes post-arrival. Rectal temperature (RT) was measured first using a mercury thermometer and a digital thermometer. Axillary temperature (AT) was measured using a digital thermometer. The tympanic membrane temperature (TMT) was measured last using an infrared thermometer. Complete blood count (CBC) profiles, heart rates and respiratory rates of animals were within normal range. The difference between RT and AT ranged from 0.1°C to 1.1°C, whereas that between RT and TMT ranged from 0.03°C to 2.1°C. 40% of temperatures measured by AT and 90% of those measured with TMT differed by more than 0.5°C from those measured by RT. AT and TMT were positively correlated with RT (r = 0.833, P < 0.0001) and (r = 0.475, P = 0.035) respectively. AT and TMT were weakly correlated (r = 0.542, P = 0.014). The present study showed that RT shouldn’t be substituted for AT or TMT in dogs. AT and TMT cannot be used interchangeably with rectal temperature in dogs.
狗的体温是通过直肠测量的，这在具有攻击性或无直肠疾病的动物中很难获得。还开发了其他工具和路线。这些可供选择的方法可能产生不同的结果。本研究旨在比较测量狗体内温度的不同方法。转到小动物诊所进行一般健康检查的病例(n=20)在确认其健康状况后被纳入本研究。30分钟后测量腋窝、鼓膜和直肠温度。直肠温度(RT)首先使用水银温度计和数字温度计测量。腋窝温度(AT)采用数字体温计测量。最后用红外测温仪测量鼓膜温度。动物全血细胞计数(CBC)、心率和呼吸频率均在正常范围内。RT和AT之间的差异范围为0.1°C至1.1°C，而RT和TMT之间的差异范围为0.03°C至2.1°C。40%的AT测量温度和90%的TMT测量温度与RT测量温度相差大于0.5°C, AT和TMT分别与RT呈正相关(r = 0.833, P < 0.0001)和(r = 0.475, P = 0.035)。AT与TMT呈弱相关(r = 0.542, P = 0.014)。目前的研究表明，RT不应该取代狗的AT或TMT。AT和TMT不能与狗的直肠温度互换使用。
{"title":"Assessment of Thermometry Practices in Dogs Undergoing General Health Examinations","authors":"Ahmed Khayri, H. Hussein, Rehab Sayed, N. Waly","doi":"10.21608/avmj.2023.203699.1140","DOIUrl":"https://doi.org/10.21608/avmj.2023.203699.1140","url":null,"abstract":"Dogs’ temperature is measured through the rectum, which can be difficult to obtain in animals with aggressive nature, or those with ano-rectal conditions. Alternative instruments and routes have also been developed. These alternative methods may yield different results. This study aimed to compare different methods in measuring the internal temperature of dogs. Cases referred to the small animal clinic (n=20) for a general health check were included in this study upon confirming their health status. Axillary, tympanic membrane and rectal temperature were measured 30 minutes post-arrival. Rectal temperature (RT) was measured first using a mercury thermometer and a digital thermometer. Axillary temperature (AT) was measured using a digital thermometer. The tympanic membrane temperature (TMT) was measured last using an infrared thermometer. Complete blood count (CBC) profiles, heart rates and respiratory rates of animals were within normal range. The difference between RT and AT ranged from 0.1°C to 1.1°C, whereas that between RT and TMT ranged from 0.03°C to 2.1°C. 40% of temperatures measured by AT and 90% of those measured with TMT differed by more than 0.5°C from those measured by RT. AT and TMT were positively correlated with RT (r = 0.833, P < 0.0001) and (r = 0.475, P = 0.035) respectively. AT and TMT were weakly correlated (r = 0.542, P = 0.014). The present study showed that RT shouldn’t be substituted for AT or TMT in dogs. AT and TMT cannot be used interchangeably with rectal temperature in dogs.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131141506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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