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Biodiversity indices for the Futog park (Novi Sad, Serbia) Futog公园(Novi Sad, Serbia)生物多样性指数
Pub Date : 2021-01-01 DOI: 10.2298/zmspn2140127l
Milena Lakicevic, S. Orlović, Ksenija Hiel, Srdjan Seremesic, Radenka Kolarov, Milena Maksimovic
Biodiversity and biodiversity preservation are some of the most important topics in ecology. Protecting biodiversity in urban ecosystems is especially challenging. Urban parks are a valuable part of green infrastructure in cities, as they contribute to preserving natural habitats for many species. Biodiversity can be quantified by calculating different diversity indices, and in this paper, we have calculated alpha indices (Shannon, Simpson, Fisher, Berger-Parker and Margalef index) and beta indices (Sorensen index, Jaccard distance and Bray-Curtis index) for the Futog park in Novi Sad, Serbia, within two periods, 2005 and 2020. The goal of the paper is to analyze the current values of biodiversity indices in the park and compare them with the ones from 15 years ago. In addition to the analysis of biodiversity indices values, the paper provides the comparison of taxonomic structure of flora, and the abundance of native, endemic and invasive species in the park, in 2005 and 2020. The results show that the values of biodiversity indices have decreased in the indicated period of time, and one of the main causes can be related to the spread of invasive species within the park. For calculation purposes, we have used the R program and the R package ?vegan?.
生物多样性和生物多样性保护是生态学中最重要的话题之一。保护城市生态系统的生物多样性尤其具有挑战性。城市公园是城市绿色基础设施的重要组成部分,因为它们有助于保护许多物种的自然栖息地。生物多样性可以通过计算不同的多样性指数来量化,本文以塞尔维亚诺维萨德Futog公园为研究对象,分别计算了2005年和2020年两个时期的alpha指数(Shannon、Simpson、Fisher、Berger-Parker和Margalef指数)和beta指数(Sorensen指数、Jaccard距离和Bray-Curtis指数)。本文的目的是分析该公园生物多样性指数的现状,并与15年前的生物多样性指数进行比较。在生物多样性指数分析的基础上,对2005年和2020年的植物区系结构、本地、特有种和入侵物种的丰度进行了比较。结果表明,生物多样性指数在一定时期内呈下降趋势,其主要原因之一可能与入侵物种在公园内的扩散有关。为了便于计算,我们使用了R程序和R软件包。
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引用次数: 0
Ambrosia trifida L. (Giant ragweed) 大豚草(Ambrosia trifida L.)
Pub Date : 2021-01-01 DOI: 10.2298/zmspn2141035s
A. Savić
Ambrosia trifida L. belongs to the group of invasive weeds, which in colonized areas cause great damage to the biodiversity of autochthonous flora and vegetation as well as in plant production. It originates from North America wherefrom it has spread to many parts of Europe. In Serbia it is currently locally present in the area of Backa (the northern region of the country). Early and rapid growth rate, vegetative and generative production, high degree of morphological and reproductive plasticity have given it a competitive advantage over many other weeds, hence in many countries it is considered one of the most problematic weeds in agricultural production. A. trifida could cause great damage in root crops, vegetable gardens and orchards and its harmfulness is measured by the negative impact on biodiversity by suppressing indigenous and other non-indigenous species. With its allergens, A. trifida negatively affects human health. Observing its vegetative and generative potential and climate change on the other hand, recent research indicates the potential for the spread of A. trifida in our country and in Europe, which could be a serious risk for agrophytocenoses and the ecosystem as a whole. In 2019, it was added to the EPPO A2 List of quarantine pests recommended. It can be controlled with the use of mechanical, biological and chemical measures.
三叶草(Ambrosia trifida L.)属入侵杂草,在殖民地地区对本土植物和植被的生物多样性以及植物生产造成极大的破坏。它起源于北美,并从那里传播到欧洲的许多地方。在塞尔维亚,目前在当地的Backa地区(该国北部地区)存在这种疾病。早期和快速的生长速度,营养和生殖生产,高度的形态和生殖可塑性使其比许多其他杂草具有竞争优势,因此在许多国家,它被认为是农业生产中最具问题的杂草之一。三叶草对根茎作物、菜园和果园的危害很大,其危害程度主要是通过抑制本地和其他非本地物种对生物多样性的负面影响来衡量的。由于其过敏原,三叶草对人类健康有负面影响。另一方面,通过观察其营养和生殖潜力以及气候变化,最近的研究表明,三叶草在我国和欧洲的传播潜力,可能对农业植物和整个生态系统构成严重威胁。2019年,它被添加到EPPO推荐的检疫性害虫A2清单中。它可以通过机械、生物和化学措施加以控制。
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引用次数: 0
Cyanobacteria and algae from biofilm at the entrance zone of Petnica Cave 佩特尼察洞穴入口生物膜中的蓝藻和藻类
Pub Date : 2021-01-01 DOI: 10.2298/zmspn2140071p
Sladjana Popovic, K. Petrović, Dusica Trnavac-Bogdanovic, D. Milošević, Ana Graovac, Ivana Trbojević, G. Subakov-Simić
The importance of biofilms in caves, the diversity of microorganisms in them, their mutual relationship and relationship with the substratum are among the advancing research topics in microbial biospeleology. This research is making contribution to the knowledge about biofilms at cave entrances and phototrophic communities in them. In that manner, biofilms from the entrance zone of the Petnica Cave were examined. Light microscopy showed that cyanobacteria were exclusively dominant phototrophs (34 taxa out of 39 total taxa recorded) with coccoid forms prevailing (28 taxa); simple trichal forms were present to a lesser extent, while heterocytous ones were completely absent. Genera Gloeocapsa, Chroococcus,Gloeothece and Leptolyngbya were the most diverse. Four green algal genera characteristic for aerophytic habitats (Apatococcus, Desmococcus, Haematococcus and Trentepohlia) were also recorded, while Bacillariophytawere observed sporadically. Three groups of sampling sites were distinguished based on recorded taxa, their richness and similarity, using non-metric multidimensional scaling (NMDS). Quantitative biofilm characteristics were also assessed - the content of chlorophyll a (Chl a) was determined, as well as the contents of water, organic and inorganic matter. Chl a had a significant positive correlation with the content of organic matter (r=0.904, P=0.013).
生物膜在洞穴中的重要性、洞穴中微生物的多样性、它们之间的相互关系以及与基质的关系是微生物生物洞穴学研究的前沿课题。本研究对洞窟入口生物膜及其光养群落的认识有一定的贡献。以这种方式,从佩特尼察洞穴的入口区域的生物膜进行了检查。光镜观察显示,39个分类群中有34个是蓝藻,28个是球藻;简单的三分体形式较少,而异细胞形式完全不存在。Gloeocapsa属、choococcus属、Gloeothece属和leppolylybya属的种类最多。此外,还发现了4种绿藻属(绿球菌、粘球菌、红球菌和Trentepohlia),偶见硅藻属。采用非度量多维尺度(NMDS)方法,根据所记录的分类群、丰富度和相似性对3组采样点进行了区分。定量评价了生物膜的特性——测定了叶绿素a (Chl a)的含量,以及水分、有机物和无机物的含量。Chl a与有机质含量呈极显著正相关(r=0.904, P=0.013)。
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引用次数: 1
Iodine and the higher plants 碘和高等植物
Pub Date : 2021-01-01 DOI: 10.2298/zmspn2141119k
R. Kastori, I. Maksimović, M. Putnik-Delić
Iodine (I) is widely distributed in the biosphere in small concentrations. Iodide (I-) and iodate (IO3-) are the most important inorganic forms of I that can be found in the biosphere. Iodine is a necessary microelement for some marine algae and higher animals. Approximately 35% of the world?s population is insufficiently provided with I, which results in many health problems. Plants are an important source of I for humans, and the knowledge about the uptake, distribution, and accumulation of I in edible plant parts, as well as its effect on life processes and organic production of cultivated species is very important. The results of numerous studies indicate a favorable effect of I application on plant growth, tolerance to stress, and the antioxidant capacity of plants. In addition, its effects on photosynthesis, hydration, carbohydrate metabolism, respiration, and interaction with other elements are analyzed. At the same time, data are indicating that the application of I has no effect, moreover, it may act phytotoxically, especially at higher concentrations. Based on the current understanding of I impact on life processes and the organic production of higher plants, it can be considered a useful element. Plants can complete their life cycle without iodine, but it may have a stimulating effect on their growth under certain circumstances. Considering the importance of I for the health of the human population and higher animals, the application of I to increase its concentration in edible parts of grown plants using cultivation practices (biofortification of crops with I) is an important task for further research.
碘(I)在生物圈中以小浓度广泛分布。碘化物(I-)和碘酸盐(IO3-)是生物圈中最重要的无机形式。碘是一些海藻和高等动物必需的微量元素。大约占世界的35% ?中国的人口得不到充分的免疫接种,这导致了许多健康问题。植物是人类重要的I来源,了解I在可食用植物部位的吸收、分布和积累及其对栽培物种的生命过程和有机生产的影响是非常重要的。大量研究结果表明,I的施用对植物的生长、抗逆性和抗氧化能力都有良好的影响。此外,还分析了其对光合作用、水合作用、碳水化合物代谢、呼吸作用以及与其他元素的相互作用的影响。与此同时,数据表明,I的施用没有效果,而且,它可能具有植物毒性,特别是在高浓度时。根据目前对I对生命过程和高等植物有机生产的影响的理解,它可以被认为是一个有用的元素。植物可以在没有碘的情况下完成它们的生命周期,但在某些情况下,碘可能对它们的生长有刺激作用。考虑到维生素I对人类和高等动物健康的重要性,利用种植方法(含维生素I的作物生物强化)应用维生素I提高其在植物可食用部位的浓度是进一步研究的重要任务。
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引用次数: 1
Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum 由膨胀青霉引起的塞尔维亚柑桔采后腐烂
Pub Date : 2021-01-01 DOI: 10.2298/zmspn2140029s
S. Stošić, D. Ristic, S. Zivkovic
Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 ?C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
柑桔是柑橘属中最受欢迎的水果之一。人们食用它们是因为它们的营养和健康益处,以及令人愉悦的味道和气味。本文报道了从柑桔模制果实中分离得到的膨胀青霉的鉴定和性质。获得的分离株在5种培养基(CYA)、MEA(麦芽提取物琼脂)、CREA(肌酸蔗糖琼脂)、YES(酵母提取物蔗糖琼脂)和OA(燕麦琼脂)上和5种不同的培养温度(5、15、25、30和37℃)下进行培养。对分离物进行了两个分子位点的测序:内部转录间隔物和β -微管蛋白。从形态、生理、分子和系统发育等方面对分离物进行了鉴定。经致病性试验证实该分离种对柑桔具有致病性。据我们所知,这是在塞尔维亚首次报道柑橘果实采后病原菌。
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引用次数: 0
Program R in mapping of protected natural assets in Serbia 绘制塞尔维亚受保护自然资产的R方案
Pub Date : 2020-01-01 DOI: 10.2298/ZMSPN2039115L
D. Lakicevic
This paper deals with the tasks of creating maps of protected natural assets (national parks and natural monuments - individual plant specimens) on several case examples in Serbia. The main goal of the paper is to introduce the application of the R program in diverse mapping assignments. Even though the R program is commonly used for numerical and statistical analysis, its application in mapping assignments is still relatively new and not widely known. Therefore, this paper aims to promote the use of R in mapping and to present the appearance of maps that can be obtained as a result. The selected examples are shown on different spatial scales, starting from the mapping of national parks on a map of Serbia, continuing with the mapping of a single national park in a narrow spatial context, ending with the mapping of individual protected plants. The same procedure can be repeated for the similar mapping assignments in the future. Creating maps is an integral part of defining adaptive management strategies for protected natural assets, and therefore can greatly impact the process of monitoring and conducting conservation measures for in situ plant protection activities.
本文以塞尔维亚的几个案例为例,讨论创建受保护的自然资产(国家公园和自然纪念碑-个别植物标本)地图的任务。本文的主要目的是介绍R程序在各种映射作业中的应用。尽管R程序通常用于数值和统计分析,但它在映射赋值中的应用仍然相对较新,并且不广为人知。因此,本文旨在推广R在制图中的使用,并呈现由此可以获得的地图的外观。选定的例子以不同的空间尺度显示,从塞尔维亚地图上的国家公园地图开始,继续在狭窄的空间范围内绘制单个国家公园的地图,最后绘制单个受保护植物的地图。对于将来类似的映射赋值,可以重复相同的过程。绘制地图是确定受保护自然资产适应性管理战略的一个组成部分,因此可以极大地影响就地植物保护活动的监测和实施保护措施的过程。
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引用次数: 1
Possibility of using Bacillus and Trichoderma strains for decomposition of crop residues 利用芽孢杆菌和木霉菌株分解作物残余物的可能性
Pub Date : 2020-01-01 DOI: 10.2298/zmspn2038051b
Dragana Bjelic Dj., B. Marinković, B. Tintor, M. Ninkov, R. Vasin, S. Zivanov, P. Jaksic
The objective of this study was to investigate the possibility of using microbial strains as residue decomposers and to determine the effect of these strains on chemical and microbial properties in the residue-amended soil. Greenhouse experiment consisted of eight Bacillus treatments, three Trichoderma treatments, and their combination, all applied to non-sterile chernozem soil amended with wheat straw. Incorporation of wheat straw improved soil chemical and microbial properties, while the extent of residue decom?position under microbial strains was intensified. Microbial treatments significantly affected the soil pH, the content of carbonate, total carbon, soil organic carbon, humus, and available phosphorus and potassium. Bacterial and fungal treatments also significantly influenced the total microbial number, ammonifiers, N2-fixers, fungi, actinomycetes, oligotrophs, copiotrophs, and cellulolytic microorganisms. The effect of microbial treatments varied depending on the applied strains and examined properties, with Bacillus strains being more promising residue decomposers compared to Trichoderma strains. The most effective microbial strains could be used as potential decomposers of crop residues.
本研究的目的是探讨利用微生物菌株作为残渣分解者的可能性,并确定这些菌株对残渣改良土壤的化学和微生物特性的影响。温室试验包括8个芽孢杆菌处理、3个木霉处理及其组合,均施用于麦秸改良的黑钙土。麦秸的掺入改善了土壤的化学性质和微生物性质,而秸秆的腐烂程度?在微生物菌株下的位置加强。微生物处理对土壤pH、碳酸盐、全碳、土壤有机碳、腐殖质、速效磷和速效钾含量均有显著影响。细菌和真菌处理也显著影响了微生物总数、氨化菌、n2固定菌、真菌、放线菌、寡养菌、共养菌和纤维素降解微生物。微生物处理的效果取决于所施用的菌株和所检查的性质,与木霉菌株相比,芽孢杆菌菌株是更有希望的残渣分解者。最有效的微生物菌株可以作为作物残茬的潜在分解者。
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引用次数: 0
Effect of Trichoderma harzianum on morpho-physiological parameters and metal uptake of tomato plants 哈兹木霉对番茄植株形态生理参数及金属吸收的影响
Pub Date : 2020-01-01 DOI: 10.2298/ZMSPN2039061V
Igor Vukelić, Gordana Racić, Mirjana Bojović, N. Curcic, D. Mrkajić, L. Jovanovic, D. Panković
In this study we have investigated the effect of T. harzianum on growth, content of chlorophyll and epidermal flavonols and metal distribution in tomato plants. Trichoderma strain, isolated from the A horizon (5-30 cm) of agricultural soil used in organic production, was applied near the root in the sixth leaf development phase of tomato. Tomato plants were grown in a growth cabinet up to the stage of 10 leaves. Content of chlorophyll (Chl), epidermal flavonols (Flav) and antocyanins (Ant) were measured in vivo non-destructively. The concentration of Cd, Co, Cu, Cr, Fe, Mn, Ni, Se, and Zn was measured in different parts of tomato plants by ICP-OES method. Results have shown that Trichoderma application positively affected growth of tomato plants, and significantly decreased nitrogen balance index. In Trichoderma treated plants the contents of Cr and Ni were significantly reduced in roots, while content of Cd tended to decrease in all plant parts, significantly in roots. Presented results indicate that investigated isolate is worthwhile testing for plant growth promotion in field conditions, taking in account different supply of macro and micronutrients.
本文研究了哈兹纳姆(T. harzianum)对番茄植株生长、叶绿素含量、表皮黄酮醇含量及金属分布的影响。从有机农业土壤A层(5 ~ 30 cm)分离得到木霉菌株,在番茄第6叶发育阶段就近施用。番茄植株在生长柜中生长,直到长出10片叶子。在体内无损检测了叶绿素(Chl)、表皮黄酮醇(Flav)和抗花色素(Ant)的含量。采用ICP-OES法测定了番茄植株不同部位Cd、Co、Cu、Cr、Fe、Mn、Ni、Se和Zn的浓度。结果表明,施用木霉对番茄植株生长有积极影响,并显著降低氮素平衡指数。木霉处理植株根系中Cr和Ni含量显著降低,而Cd含量在植株各部位均有降低的趋势,其中以根系下降最为显著。结果表明,在所研究的分离物中,考虑到宏量和微量营养元素的不同供应,值得在田间条件下进行促进植物生长的试验。
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引用次数: 1
Biological spectrum of the weed flora in the Vrsac vineyards (Serbia) 塞尔维亚Vrsac葡萄园杂草区系的生物学谱
Pub Date : 2020-01-01 DOI: 10.2298/ZMSPN2039101A
A. Andjelković, D. Marisavljević, Dušanka Cvijanović, S. Radulović, D. Pavlović
Agrotechnical measures are the main factor defining the vineyard weed flora structure and composition, while adequate weed control measures simultaneously ensure that vineyards are being well-managed, thus securing good grapevine health and high quality of wine. Given that the biological spectrum of weeds affects the choice of weed control measures, the aim of this study was to determine the biological properties of the weed flora in Vrsac vineyards, by assessing dominant life forms and phenology of the identified weeds. The floristic analysis was conducted during the 2016 vegetation season (March-November) at 60 plots (1 m2), at three field sites. The presence of 97 plant taxa, belonging to 26 families, was determined. The biological spectrum of the vineyards weed flora has shown a thero?phyto-hemicryptophyte character (therophytes: 57.73% and hemicryptophytes: 34.02%). The scapose herbaceous plants with summer-flowering phenology were dominant within the therophytes and hemicryptophytes. The obtained results have shown a higher weed diversity in vineyards, when compared to previous research of the weed flora in the study area, but similar to more recent studies conducted in the neighbouring countries. Furthermore, the dominant presence of therophytes in the vineyard weed flora was expected, bearing in mind the primarily mechanical weed control measures traditionally applied in vineyards.
农业技术措施是决定葡萄园杂草结构和组成的主要因素,而适当的杂草控制措施同时确保葡萄园得到良好的管理,从而确保良好的葡萄藤健康和高品质的葡萄酒。鉴于杂草的生物光谱影响杂草控制措施的选择,本研究的目的是通过评估已鉴定杂草的优势生命形式和物候,确定Vrsac葡萄园杂草区系的生物学特性。在2016年植被季(3 - 11月)对3个样地的60个样地(1 m2)进行了区系分析。共鉴定出26科97个植物类群。葡萄园杂草植物群的生物光谱显示出了一个巨大的变化。植物半隐性(植生:57.73%,半隐性:34.02%)。在旱生植物和半旱生植物中,夏花物候的腐生草本植物占主导地位。与之前对研究区域杂草区系的研究相比,获得的结果表明,葡萄园中的杂草多样性更高,但与最近在邻国进行的研究相似。此外,考虑到传统上在葡萄园中应用的主要机械杂草控制措施,预计植物在葡萄园杂草区系中占主导地位。
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引用次数: 0
Soil organic carbon fractions in different land use systems of Chernozem soil 黑钙土不同土地利用方式土壤有机碳组分
Pub Date : 2020-01-01 DOI: 10.2298/zmspn2038031s
Srđan Šeremešić, L. Nešić, V. Ćirić, J. R. Vasin, I. Đalović, J. Marinković, B. Vojnov
The relationship between soil carbon fractions in Chernozem soils was assessed in soil samples of three different environments: arable soil, grassland and oak for?est. Grassland and oak forest had higher soil organic carbon (SOC), carbon soluble in hot water (HWC), particulate organic carbon (POC) and mineral-associated carbon (MOC) than the arable soil. The POC/MOC ratio was lowest in arable soil, indicating a smaller carbon pool for microbial turnover. POC increases with higher total SOC, indicating that the pres?ervation of organic matter depends on the renewal of labile fractions. Our results showed that fertilization had active role in soil carbon stabilization, while crop rotation had less effect on a soil carbon turnover. Our result could contribute to the better understanding of SOC fractions composition and relevance in Chernozem soil, thus could help in selection of cropping management systems for SOC preservation.
以黑钙土为研究对象,在耕地、草地和栎树3种不同环境下,评价了黑钙土土壤碳组分之间的关系。草地和栎林土壤有机碳(SOC)、热溶碳(HWC)、颗粒有机碳(POC)和矿物伴生碳(MOC)均高于耕地土壤。耕地土壤的POC/MOC最低,表明微生物周转的碳库较小。POC随着总SOC的增加而增加,表明压力?有机物的观测依赖于不稳定组分的更新。结果表明,施肥对土壤碳稳定有积极作用,而轮作对土壤碳周转影响较小。研究结果有助于更好地了解黑钙土有机碳组分的组成及其相关性,从而有助于选择有利于有机碳保存的耕作管理制度。
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引用次数: 1
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Zbornik Matice Srpske za Prirodne Nauke
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