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Characteristics of agricultural organic matter degrading bacterial isolates from different types of soil 不同类型土壤中农业有机质降解菌分离物的特性
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936069n
D. B. Najvirt, S. Djuric, I. T. Hajnal-Jafari, R. Stamenov
A large amount of agricultural organic matter (AOM) comes into soil every day, through organic remains, and it is decomposed by bacteria, fungi and actinomycetes (Ugarkovic et al, 2011). The aim of this research was to select isolates of bacteria with the most organic matter degrading potential, by isolating the bacteria from five different types of soil. Isolation of bacteria was conducted from five types of soil - luvisol, cambisol, chernozem, forest land and meadow. Characterization of bacterial isolates was conducted based on morphological, physiological and biochemical features. Isolates with the most organic matter degrading potential could be used in the near future for conceptualizing microbiological preparation.
大量的农业有机质(AOM)每天通过有机残留物进入土壤,并被细菌、真菌和放线菌分解(Ugarkovic et al ., 2011)。本研究的目的是通过从五种不同类型的土壤中分离细菌,选择具有最大有机物降解潜力的细菌分离物。从5种土壤中分离细菌,分别是草甸土壤、黑钙土、林地土壤和草甸土壤。根据形态、生理生化特征对分离菌株进行鉴定。具有最大有机物降解潜力的分离物可以在不久的将来用于概念化微生物制剂。
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引用次数: 0
Urinary tract infections and their importance 尿路感染及其重要性
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936173s
G. Smieško, M. Mikov, Vera Gusman, M. Djanic
Urinary tract infections (UTI) are one of the most common infectious diseases, primarily caused by bacteria present in the intestinum, affecting the entire urinary tract or only a part of it. If the urinary bladder and urethra are affected then they are considered ?lower? urinary tract infections, and the affected kidneys and ureter are considered ?upper? urinary tract infections. There is a division into uncomplicated and complicated UTIs. Approximately 15% of all prescribed antibiotics in the United States are prescribed as therapy of UTIs. The UTI data from other countries are similar. The costs incurred in the treatment of these infections are significant - in the United States, the direct costs of UTI treatments are estimated at $ 1.6 billion per year.
尿路感染(UTI)是最常见的传染病之一,主要由存在于肠道中的细菌引起,影响整个尿路或仅影响部分尿路。如果膀胱和尿道受到影响,则认为它们较低?尿路感染,以及受影响的肾脏和输尿管被认为是上?尿路感染。有简单和复杂的uti之分。在美国,大约15%的抗生素处方用于治疗尿路感染。其他国家的尿路感染数据也类似。治疗这些感染的费用很高——在美国,尿路感染治疗的直接费用估计为每年16亿美元。
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引用次数: 0
Mercury content and distribution in household dust and soil in the town of Sid 锡德镇家庭灰尘和土壤中的汞含量和分布
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1937033n
A. Nedić, M. Pucarević, Jordana Ninkov, N. Stojić, D. Milić
Mercury (Hg) and potentially toxic elements (PTEs) are components of household dust and are a risk for human health. The aim of this study was to determine the concentrations of Hg and PTEs in household dust in individual housing facilities in the town of Sid, Serbia and their correlation to the content of the elements found in the surrounding garden soil. Total of 64 samples of household dust were collected from 16 locations of individual housing facilities on 4 occasions. Samples of surrounding yard and garden soil were simultaneously collected. None of the 64 analysed soil samples exceeded the threshold limit (TL) prescribed by law for non-agricultural soil, which is 0.3 mg kg-1 DM. Content of Hg in household dust was much higher than in the surrounding soil and ranged from 0.005 to 1.566 mg kg-1 DM. The resulting values of PTEs (As, B, Co, Cr, Cu, Ni, Pb, and Zn) in household dust had a significantly higher range than in the soil. Contents of Hg, B, Cu, and Zn were much higher in household dust than in the soil. According to the results of correlations of the analysed elements in household dust and surrounding soil, and according to the analysed locations, direct effect of Hg and PTEs contents on the content of the same elements in household dust was not confirmed.
汞(Hg)和潜在有毒元素(pte)是家庭灰尘的组成部分,对人类健康构成威胁。本研究的目的是确定塞尔维亚锡德镇个别住房设施的家庭灰尘中汞和pte的浓度及其与周围花园土壤中发现的元素含量的相关性。在个别住宅设施的16个地点共4次收集了64个家居尘埃样本。同时采集周围庭院和花园土壤样品。64个土壤样品均未超过非农业土壤的法定阈值(0.3 mg kg-1 DM)。家庭粉尘中Hg的含量远高于周围土壤,范围为0.005 ~ 1.566 mg kg-1 DM。家庭粉尘中pte (As、B、Co、Cr、Cu、Ni、Pb和Zn)的结果值范围显著高于土壤。家庭粉尘中Hg、B、Cu、Zn的含量明显高于土壤。根据分析的家庭粉尘与周围土壤元素的相关性结果,并根据分析的地点,没有确定Hg和PTEs含量对家庭粉尘中相同元素含量的直接影响。
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引用次数: 3
Influence of salinity on the growth and development of pansies (Viola x wittrockiana Gams.) 盐度对三色堇(Viola x wittrockiana Gams.)生长发育的影响
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1937057p
G. M. Pusic, M. Mladenovic, D. J. Cukanovic, D. Lakicevic, M. Pavlović
The purpose of this paper is to determinate the influence of salinity on the growth and development of pansies (Viola x wittrockiana Gams.). The objective of the present study was to examine the cultivation area selection and use of pansies related to the autumn and winter flowering aspects. A total of 40 pansy seedlings were included in the study and allocated to four groups of ten each. The experiment conducted four involved treatments: control (K), treatment with 3g/l NaCl (T1), treatment with 5g/l NaCl (T2), and treatment with 7 g/l NaCl (T3). Concentrated water was used for watering the seedlings observed during growth. Over the course of the eight-week experiment, the following quantitative and qualitative properties of the pansy seedlings were measured: height, number of leaves, number of flowers, diameter of flowers, length of leaves, width of leaves, as well as the vitality and decorativeness of the pansy seedlings. The results obtained show that pansies can tolerate increased amounts of salt (5 g/l and 7 g/l NaCl) with adverse effects on the growth, development, vitality and decorativeness of the plant.
本文的目的是确定盐度对三色堇(Viola x wittrockiana Gams.)生长发育的影响。本研究的目的是探讨三色堇在秋冬开花方面的种植面积选择和利用。40株三色堇幼苗被分为四组,每组10株。试验分为对照(K)、3g/l NaCl处理(T1)、5g/l NaCl处理(T2)和7g /l NaCl处理(T3) 4个处理。用浓水浇灌生长过程中观察到的幼苗。在为期8周的实验过程中,对三色堇幼苗的高、叶数、花数、花径、叶长、叶宽以及幼苗的活力和装饰性进行了定量和定性的测量。结果表明,三色堇可以耐受5 g/l和7 g/l NaCl浓度的增加,对植株的生长发育、活力和装饰性有不利影响。
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引用次数: 2
Charophytes of Gornje Podunavlje ponds: Revitalization process aspect 果仁池绿藻:再生过程方面
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936123t
Ivana Trbojević, D. Predojević, J. Šinžar-Sekulić, N. Nikolić, Ivana Jovanović, G. Subakov-Simić
Unique marshland area in the Danube floodplain - Special Nature Re?serve Gornje Podunavlje is considered to be one of the last aquatic flora and fauna refuges in the region. The revitalization projects in SNP Gornje Podunavlje started in 2011. They were conducted in order to protect biodiversity and to provide the natural ecosystem. The aim of this study was to investigate and compare the present diversity and distribution of the charophytes in five ponds of SNR Gornje Podunavlje, depending on phases of the revitalization process within the ponds. The researched area includes the following ponds: Semenjaca, Sarkanj, Siroki rit, Sakajtas and Ribolov. The Siroki rit pond was under the process of revitalization during the field surveys, Semenjaca and Sarkanj have already been revitalized, and Ribolov and Sakajtas were not exposed to the revitalization processes at all. The field surveys were conducted monthly, from May to September 2016. Water quality parameters were measured in situ and in laboratory. Ponds differed considerably when it comes to water chemistry; Semenjaca and Sarkanj were associated with highest nutrient concentrations, while Siroki rit was characterized by highest conductivity and water hardness. The highest diversity was found in Siroki rit pond, which was still in process of revitalization when the field survey was conducted. Charophytes were not detected within previously revitalized ponds (Semenjaca and Sarkanj). Eight species of charophytes were detected: Chara contraria, Chara globularis, Chara tenuispina, Chara vulgaris, Nitella confervacea, Nitella gracilis, Nitella syncarpa and Tolypella prolifera.
多瑙河洪泛区独特的沼泽地-特殊自然保护区?Gornje Podunavlje被认为是该地区最后的水生动植物保护区之一。SNP Gornje Podunavlje的复兴项目始于2011年。它们是为了保护生物多样性和提供自然生态系统而进行的。本研究的目的是调查和比较SNR Gornje Podunavlje 5个池塘中根据池塘内恢复过程的不同阶段的蕨类植物的多样性和分布。研究区域包括:Semenjaca、Sarkanj、Siroki rit、Sakajtas和Ribolov。在实地调查中,Siroki池塘处于复兴过程中,Semenjaca和Sarkanj已经恢复了活力,而Ribolov和Sakajtas根本没有受到复兴过程的影响。2016年5月至9月,每月进行实地调查。对水质参数进行了现场和室内测量。池塘在水化学方面有很大的不同;Semenjaca和Sarkanj具有最高的营养浓度,而Siroki rit具有最高的电导率和水硬度。在进行实地调查时,仍处于恢复过程中的西罗基池塘的多样性最高。在以前恢复活力的池塘(Semenjaca和Sarkanj)中未发现叶绿植物。共检出8种蕨类植物,分别为:反蕨蕨、球状蕨蕨、细毛蕨蕨、寻常蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨蕨。
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引用次数: 3
The influence of nitrogen and growth phase on the toxicity of the cyanobacterial strain Microcystis PCC 7806 氮和生长阶段对微囊藻pcc7806毒性的影响
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936089d
Petar Davidović, Dajana J. Blagojević, O. Babić, J. Simeunović
The worldwide occurrence of toxic cyanobacterial blooms and their numerous harmful effects have instigated extensive research into the environmental conditions promoting such events. Among the environmental factors which have been suggested to influence the increase in cyanobacterial proliferation, nutrient levels have been identified as one of the most prominent, affecting the growth and toxic metabolite production of cyano?bacteria in freshwater ecosystems. In the present study, toxicity of the cyanobacterial strain Microcystis PCC 7806 was evaluated after growth in media with three different nitrogen concentrations. The toxicity of intracellular extracts was analyzed during different growth phases (after 7, 21, and 35 days of cultivation) by observing mortality rates in the Artemia salina bioassay after 24h and 48h of exposure. The results have not shown significantly higher mortality levels between the test organisms exposed to extracts obtained from the cultures grown in the presence of higher nitrogen content (1.5 g/l and 0.8 g/l) and those grown in a nitrogen-free medium. A dose dependent effect, however, can be observed in most cases, with the most substantial changes observed in the high-dose groups. Also, the toxic effects and larval mortality increased during the exposure, suggesting the time-dependent toxicity. Extracts obtained after longer periods of cultivation (21 and 35 days) had stronger effects on the test organisms, which indicates that the toxicity of the tested cyanobacterial strain depends on the specific growth phase.
世界范围内发生的有毒蓝藻华及其众多有害影响已经激起了对促进此类事件的环境条件的广泛研究。在已提出的影响蓝藻增殖增加的环境因素中,营养水平已被确定为最突出的因素之一,影响蓝藻的生长和有毒代谢物的产生?淡水生态系统中的细菌。在本研究中,对蓝藻菌株微囊藻PCC 7806在三种不同氮浓度的培养基中生长后的毒性进行了评估。在不同的生长阶段(培养7、21和35天),通过观察暴露24小时和48小时后盐蒿生物测定中的死亡率来分析细胞内提取物的毒性。结果显示,暴露于高氮含量(1.5 g/l和0.8 g/l)培养物提取液的试验生物体与在无氮培养基中培养的试验生物体之间的死亡率水平没有显著提高。然而,在大多数情况下可以观察到剂量依赖效应,在高剂量组观察到最显著的变化。在暴露过程中,毒性作用和幼虫死亡率增加,表明毒性具有时间依赖性。经过较长时间培养(21天和35天)获得的提取物对试验生物的影响更强,这表明被试蓝藻菌株的毒性取决于特定的生长阶段。
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引用次数: 1
Antioxidant potential of ragweeds: Ambrosia artemisiifolia, A. trifida and Iva xanthifolia 豚草的抗氧化潜力:豚草、三叶草和苍耳草
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936155k
Biljana Kiprovski, D. Malencic, J. Luković, P. Boža
The purpose of this study was to analyze antioxidant systems among three invasive ragweed species, Ambrosia artemisiifolia L., A. trifida L. and Iva xanthifolia Nutt. Antioxidant capacity could be a possible marker of adaptation to variable environmen?tal conditions, since change in amount of antioxidants represents one of the first responses to various environmental stimuli. Among investigated ragweeds, I. xanthifolia leaves had more pronounced guaiacol peroxidase activity (87.5 and 62.5%) and reduced glutathione content (2.3 and 28.8%) than A. artemisiifolia and A. trifida, respectively. However, super?oxide dismutase activity was invariable in all investigated plants (234.1-247.5 U g-1 fresh weight). The highest content of total phenolics, tannins, flavonoids and proanthocyanidins were detected in A. trifida leaves (up to 3.7 - fold the amount of the others). According to antioxidant activity tests, investigated ragweed species could be presented in a scale: A. trifida > I. xanthifolia > A. artemisiifolia. Accumulation of non-enzymatic antioxidants and lower content of reduced glutathione point to different oxidative stress avoidance strategies of A. trifida when compared to A. artemisiifolia and I. xanthifolia within the same environ?mental conditions.
研究了豚草(Ambrosia artemisiifolia L.)、三叶草(A. trifida L.)和黄叶草(Iva xanthifolia Nutt)三种入侵豚草的抗氧化系统。抗氧化能力可能是适应不同环境的标志?因为抗氧化剂量的变化是对各种环境刺激的第一反应之一。在所调查的豚草中,苍耳草叶片愈创木酚过氧化物酶活性(87.5和62.5%)和还原性谷胱甘肽含量(2.3和28.8%)分别高于蒿和三叶草。然而,超级吗?氧化物歧化酶活性在所有被调查植物中保持不变(234.1 ~ 247.5 U g-1鲜重)。三叶草叶片中总酚类、单宁类、黄酮类和原花青素的含量最高,为其他品种的3.7倍。根据抗氧化活性测试,所研究的豚草种类可按比例划分为:三叶草、黄叶草、黄叶草、蒿草。非酶促抗氧化剂的积累和还原性谷胱甘肽的含量较低,表明在相同环境下,三叶草与蒿和黄叶草相比,其氧化应激避免策略不同。心理条件。
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引用次数: 0
Campylobacter in food production chain in Vojvodina province (Serbia) 塞尔维亚伏伊伏丁那省食品生产链中的弯曲杆菌
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936023p
J. Petrović, I. Stojanov, Vera Gusman, J. Lekić, Mirjana Štrbac, R. Ratajac, S. Medić
Enteritis campylobacterialis has an increasing trend in Serbia. Human illness usually appears as sporadic case, most commonly in children with obvious seasonality. In registered outbreaks, incriminated food was most frequently poultry meat. Campylobacter is one of the most important food borne pathogens, commonly underreported, mostly because isolation of this bacteria requires specific equipment. Since 1 January 2019, monitoring of Campylobacter in poultry carcasses is mandatory when it comes to poultry production fa?cilities in Serbia. The aim of this paper was to analyze data from Autonomous Province of Vojvodina about the prevalence of Campylobacter spp. in poultry meat and risk for human illness. Our results indicate high prevalence of Campylobacter spp. in whole food chain: poultry farms, slaughterhouses, retail and, correspondingly, high risk for consumers in Vojvodina. Measures for risk reduction of disease incidence include better bio security measures on the farm level as a main source of pathogen but also introduction of Campylo?bacter diagnostic equipment in all human diagnostic and food control laboratories.
弯曲杆菌肠炎在塞尔维亚呈上升趋势。人类疾病通常以散发病例出现,最常见于儿童,具有明显的季节性。在已登记的疫情中,受污染的食物通常是禽肉。弯曲杆菌是最重要的食源性病原体之一,通常被低估,主要是因为这种细菌的分离需要特定的设备。自2019年1月1日起,在家禽生产过程中,必须对家禽尸体中的弯曲杆菌进行监测。塞尔维亚的城市。本文的目的是分析伏伊伏丁那自治省有关禽肉弯曲杆菌流行率和人类疾病风险的数据。我们的研究结果表明弯曲杆菌在伏伊伏丁那省的家禽养殖场、屠宰场、零售等整个食物链中都有很高的患病率,相应地,对消费者来说也有很高的风险。降低疾病发病率的措施包括在农场层面采取更好的生物安全措施,因为农场是病原体的主要来源,但也引入了弯曲菌?所有人类诊断和食品控制实验室中的细菌诊断设备。
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引用次数: 0
Effect of low NaCl concentrations on the water relations of rapeseed 低NaCl浓度对油菜籽水分关系的影响
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1937067p
M. Putnik-Delić, M. Daničić, Tanja Vujanov, R. Kastori
The experiment was carried out in order to examine whether relatively low concentrations of NaCl (0.2, 0.6 and 1.2 g NaCl/L in the nutrient solution), which may appear in agricultural soils or irrigation waters, affect physiological processes in young rapeseed plants (cultivar Slavica) grown under semi-controlled conditions, on a nutrient solution. The experiment was done in 7 replications, with 8 plants per replication. Increased salinity had no influence on adaxial density of stomata, while abaxial density of stomata and specific leaf area declined with an increase in NaCl concentration in the nutrient solution. The intensity of transpiration also declined with an increase in NaCl concentration in the nutrient solution. Even though plant growth was not significantly affected by applied concentrations of NaCl, it is evident that the impact of salts is complex and it is necessary to study whether there are significant changes in plant metabolism.
本试验旨在研究农业土壤或灌溉水中较低浓度的NaCl(0.2、0.6和1.2 g NaCl/L)在半控制条件下对斯拉维卡(Slavica)油菜幼苗生长的生理过程的影响。试验共7个重复,每个重复8株。盐度升高对气孔正面密度无影响,而气孔背面密度和比叶面积随营养液中NaCl浓度的升高而下降。随着营养液中NaCl浓度的增加,蒸腾强度也呈下降趋势。尽管施用NaCl浓度对植物生长没有显著影响,但显然盐的影响是复杂的,有必要研究植物代谢是否有显著变化。
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引用次数: 1
Novel trends in fermented dairy technology 发酵乳制品技术的新趋势
Pub Date : 2019-01-01 DOI: 10.2298/zmspn1936009c
Marijana Caric Dj., D. Milanovic, D. Ilicic
Novel trends in fermented dairy technology are presented in this review paper. The application of new starter cultures (probiotics, kombucha), as well as quality improving ingredients like transglutaminase (TGase), milk protein fractions, and functional components of plant origin have been investigated by the authors worldwide. New processing techniques such as: high-pressure processing (HPP), high pressure homogenization (HPH), and ultrasonic processing (USP) are interesting because of their potential to achieve a specific and/or novel functionality or to improve the efficiency. Novel trends in fermented dairy technology contribute to the creation of various products with high nutritive value, possessing also specific functional properties. Basic health benefits of functional fermented dairy products are: biologically active peptides - ACE inhibitors and antioxidative activity. Due to the mentioned functional characteristics, these dairy products are considered to be among the most precious functional foods.
本文综述了发酵乳制品技术的最新发展趋势。新的发酵剂(益生菌,康普茶)的应用,以及提高质量的成分,如谷氨酰胺转酶(TGase),牛奶蛋白组分和植物来源的功能成分已经被世界各地的作者进行了研究。新的加工技术,如高压处理(HPP),高压均质(HPH)和超声波处理(USP)是有趣的,因为它们具有实现特定和/或新颖功能或提高效率的潜力。发酵乳制品技术的新趋势有助于创造各种具有高营养价值的产品,也具有特定的功能特性。功能性发酵乳制品的基本健康益处是:生物活性肽- ACE抑制剂和抗氧化活性。由于上述功能特点,这些乳制品被认为是最珍贵的功能食品之一。
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引用次数: 3
期刊
Zbornik Matice Srpske za Prirodne Nauke
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