Ecotoxicology and Environmental Safety最新文献_第5页

A combined study of network-based prediction and in vitro experimental validation on the procarcinogenic mechanism of propylparaben in estrogen receptor-positive breast cancer cells 对羟基苯甲酸丙酯对雌激素受体阳性乳腺癌细胞前癌机制的网络预测与体外实验验证相结合的研究

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-03 DOI: 10.1016/j.ecoenv.2025.119504

Jiaying Wen , Yingying Liang , Laiming Mo , Weiliang Sun , Yanqiu Qin , Haiying Yue , Huijun Zhu

Objective

To investigate the carcinogenic mechanisms and potential molecular targets of the environmental endocrine disruptor Propylparaben in estrogen receptor-positive breast cancer.

Methods

Based on a network toxicology strategy, this study first predicted and analyzed the physicochemical properties and in vivo/vitro toxicity of Propylparaben, screened its potential targets, and intersected them with estrogen receptor-positive breast cancer-related genes to obtain candidate toxicological targets. GO, KEGG, and PPI enrichment analyses were subsequently performed to identify key functional modules. Transcriptomic data from breast cancer were integrated, and core targets were screened using LASSO regression, SVM-RFE, and random forest algorithms. A multigene diagnostic model was then constructed, evaluated, and externally validated. Differential expression analysis, protein-level validation, and molecular docking were conducted to confirm expression patterns and binding capabilities of the core targets. Using MCF-7 cells as the in vitro model, dose-dependent Propylparaben intervention experiments were conducted to examine the transcriptional responses of the target genes. Drug sensitivity prediction, survival analysis, and GSEA-based functional annotation were further performed to evaluate the clinical potential and oncogenic mechanisms of the core targets.

Results

Propylparaben demonstrated strong estrogen receptor agonist activity, metabolic enzyme inhibition, and moderate carcinogenic risk, suggesting classical endocrine-disrupting properties. A total of 109 candidate toxicological targets associated with estrogen receptor-positive breast cancer were identified and found enriched in carcinogenic and tumor-suppressive pathways such as PI3K/AKT, mTOR, MAPK, and p53. Eight core targets were selected via machine learning, with SLC2A1 and KIF11 showing significant upregulation at both mRNA and protein levels, as well as stable binding affinities with Propylparaben. MCF-7 cell experiments confirmed their dose-dependent transcriptional upregulation upon Propylparaben treatment. Drug sensitivity analysis revealed that high expression of these targets correlated with increased sensitivity to Fulvestrant. KIF11 was predictive of neoadjuvant therapy response, while high SLC2A1 expression was significantly associated with poor survival outcomes. GSEA indicated that high expression of these genes significantly activated glycolysis, cell cycle, mTORC1, MYC, and E2F pathways, while suppressing antitumor mechanisms including p53 and complement cascades.

Conclusion

Propylparaben may promote the occurrence and progression of estrogen receptor-positive breast cancer by upregulating SLC2A1 and KIF11, activating metabolic and proliferative pathways, and simultaneously suppressing tumor-suppressive signals.

目的探讨环境内分泌干扰物对羟基苯甲酸丙酯在雌激素受体阳性乳腺癌中的致癌机制及潜在的分子靶点。方法基于网络毒理学策略，首先对对羟基苯甲酸丙酯的理化性质和体内外毒性进行预测和分析，筛选其潜在靶点，并与雌激素受体阳性乳腺癌相关基因交叉，获得候选毒理学靶点。随后进行GO、KEGG和PPI富集分析，以确定关键功能模块。整合来自乳腺癌的转录组学数据，并使用LASSO回归、SVM-RFE和随机森林算法筛选核心靶点。然后构建、评估和外部验证多基因诊断模型。通过差异表达分析、蛋白水平验证和分子对接来确定核心靶点的表达模式和结合能力。以MCF-7细胞为体外模型，进行剂量依赖性对羟基苯甲酸丙酯干预实验，检测靶基因的转录应答。进一步进行药物敏感性预测、生存分析和基于gsea的功能注释，以评估核心靶点的临床潜力和致瘤机制。结果对羟基苯甲酸丙酯具有较强的雌激素受体激动剂活性、代谢酶抑制作用和中等致癌风险，具有典型的内分泌干扰特性。共鉴定出109个与雌激素受体阳性乳腺癌相关的候选毒理学靶点，并发现它们富含致癌和肿瘤抑制通路，如PI3K/AKT、mTOR、MAPK和p53。通过机器学习选择了8个核心靶点，SLC2A1和KIF11在mRNA和蛋白质水平上均表现出显著上调，并且与对羟基苯甲酸丙酯具有稳定的结合亲和力。MCF-7细胞实验证实了它们在对羟基苯甲酸丙酯处理后的剂量依赖性转录上调。药物敏感性分析显示，这些靶点的高表达与对氟维司汀的敏感性增加相关。KIF11可预测新辅助治疗的反应，而SLC2A1的高表达与较差的生存结果显著相关。GSEA表明，这些基因的高表达显著激活糖酵解、细胞周期、mTORC1、MYC和E2F通路，同时抑制包括p53和补体级联在内的抗肿瘤机制。结论对羟基苯甲酸丙酯可能通过上调SLC2A1和KIF11，激活代谢和增殖通路，同时抑制肿瘤抑制信号，促进雌激素受体阳性乳腺癌的发生和发展。

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引用次数: 0

Ingestion of BPA-containing breast milk disturbs the cecum microbiota and intestinal barrier contributing to growth inhibition of pre-weaning rabbits 摄入含有双酚a的母乳会扰乱盲肠微生物群和肠道屏障，从而抑制断奶前家兔的生长。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119452

Qiman Yang , Kechao Chen , Lianyu Qiu , Yunduan Wang , Fenglin You , Lincheng Ye , Jie Yang , Daoliang Lan , Liangde Kuang , Wei Fu

Bisphenol A (BPA), a widely used industrial compound, is a well-known endocrine-disrupting chemical associated with various diseases. However, its impact on intestinal function and growth remains insufficiently explored. This study investigated the effects of BPA-contaminated breast milk on growth performance in pre-weaning rabbits. Lactating rabbits were divided into control and BPA-exposed groups, and BPA concentrations in their breast milk were measured and compared. The results showed that BPA was detectable in milk and was transmitted to offspring in significant quantities. To evaluate the effects of BPA on pre-weaning rabbits, growth parameters (e.g., body and organ weights), blood indicators (e.g., antioxidant capacity and hormone levels), and cecal characteristics (e.g., microbiota composition, morphology, and barrier function) were analyzed. BPA exposure in nursing mothers significantly reduced the body and liver weights of their offspring, elevated BPA levels in the cecum, decreased antioxidant capacity, and induced thyroid dysfunction. Furthermore, BPA exposure disrupted the gut microbiota composition, reduced goblet cell numbers, thickened the intestinal mucosa and wall, and triggered inflammatory responses, thereby impairing intestinal barrier function. Collectively, these findings demonstrate that BPA exposure in lactating rabbits not only harms the mothers but is also transmitted to their offspring through breast milk. BPA disrupts normal metabolic and hormonal regulation and impairs intestinal function, ultimately leading to growth inhibition. This study enhances understanding of BPA-induced toxicity and provides valuable insights for preventing BPA-related diseases in humans.

双酚A （BPA）是一种广泛使用的工业化合物，是一种众所周知的与多种疾病有关的内分泌干扰化学物质。然而，其对肠道功能和生长的影响仍未得到充分探讨。本研究探讨了bpa污染的母乳对断奶前家兔生长性能的影响。将泌乳兔分为对照组和双酚a暴露组，测量并比较其母乳中的双酚a浓度。结果表明，牛奶中可检测到双酚a，并会大量遗传给后代。为了评估BPA对断奶前家兔的影响，我们分析了生长参数（如身体和器官重量）、血液指标（如抗氧化能力和激素水平）和盲肠特征（如微生物群组成、形态和屏障功能）。哺乳期母亲暴露在双酚a环境中会显著降低其后代的体重和肝脏重量，提高盲肠中的双酚a水平，降低抗氧化能力，并诱发甲状腺功能障碍。此外，BPA暴露会破坏肠道微生物群组成，减少杯状细胞数量，增厚肠道黏膜和肠壁，引发炎症反应，从而损害肠道屏障功能。总的来说，这些发现表明，暴露在哺乳期兔子体内的BPA不仅会伤害母兔，还会通过母乳传给它们的后代。BPA破坏正常的代谢和激素调节，损害肠道功能，最终导致生长抑制。本研究增强了对双酚a毒性的认识，并为预防人类双酚a相关疾病提供了有价值的见解。

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引用次数: 0

Unveiling the role of Hedgehog signaling pathway in mitigating methylisothiazolinone-induced anxiety in zebrafish 揭示Hedgehog信号通路在缓解甲基异噻唑啉酮诱导的斑马鱼焦虑中的作用。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119507

Rui Li , Jiangbo Qu , Xinyuan Hu , Tianjia Song , JunXia Hu , Yuanqing Zhang

Methylisothiazolinone (MIT), a prevalent antimicrobial agent utilized in pharmaceuticals, cosmetics, and industrial contexts, presents potential toxicity concerns. Recent investigations have corroborated its neurotoxic effects in zebrafish and suggested involvement of the Hedgehog signaling pathway, although the specific regulatory mechanisms remain to be elucidated. In this study, transcriptome-based gene-pathway network analysis identified hhip within the Hedgehog pathway. Expression profiling and in situ hybridization revealed it is suppressed by MIT exposure and displays high expression in the zebrafish midbrain and hypothalamus. Dual-luciferase reporter assays in the zebrafish Pac2 cell line demonstrated that hhip suppresses transcription of the Hedgehog target gene gli1 and modulates reactive oxygen species (ROS) levels and oxidative stress-related gene expression. Moreover, pharmacological inhibition of the Hedgehog pathway with Ciliobrevin A (CA) during MIT exposure ameliorated anxiety-like behaviors in zebrafish, such as aberrant edge preference (thigmotaxis). This intervention downregulated Hedgehog pathway activity, elevated expression of oxidative stress genes (e.g., gpx4), reduced expression of the ferroptosis marker gene tf, attenuated the loss of Nissl bodies in brain tissue, and decreased brain iron content. These findings suggested that MIT exposure is associated with molecular signatures indicative of Hedgehog pathway activation. This cascade of molecular events is associated with subsequent anxiety-like behaviors, implying its potential contribution to the behavioral phenotype. In vitro studies using Pac2 cells further corroborated that inhibition of the Hedgehog pathway alleviates the increase in lipid peroxidation induced by the ferroptosis inducer Erastin. Collectively, these results elucidated the molecular regarding MIT-induced neurotoxicity and offer potential preventive and interventional strategies for addressing toxicity associated with this class of compounds.

甲基异噻唑啉酮（MIT）是一种普遍应用于制药、化妆品和工业环境的抗菌剂，具有潜在的毒性问题。最近的研究证实了其对斑马鱼的神经毒性作用，并提出了Hedgehog信号通路的参与，尽管具体的调控机制仍有待阐明。在本研究中，基于转录组的基因通路网络分析确定了Hedgehog通路中的hip。表达谱分析和原位杂交显示，它受到MIT暴露的抑制，在斑马鱼中脑和下丘脑中表现出高表达。斑马鱼Pac2细胞系的双荧光素酶报告基因实验表明，髋关节抑制Hedgehog靶基因gli1的转录，调节活性氧（ROS）水平和氧化应激相关基因的表达。此外，在MIT暴露过程中，Ciliobrevin A （CA）对Hedgehog通路的药理学抑制可改善斑马鱼的焦虑样行为，如异常边缘偏好（thigmotaxis）。该干预下调了刺猬通路活性，提高了氧化应激基因（如gpx4）的表达，降低了铁下沉标记基因tf的表达，减轻了脑组织中尼氏体的损失，降低了脑铁含量。这些发现表明，MIT暴露与指示Hedgehog通路激活的分子特征有关。这种分子事件级联与随后的焦虑样行为有关，这意味着它对行为表型的潜在贡献。利用Pac2细胞进行的体外研究进一步证实，抑制Hedgehog通路可减轻铁下垂诱导剂Erastin诱导的脂质过氧化增加。总的来说，这些结果阐明了麻省理工学院诱导的神经毒性的分子机制，并为解决与这类化合物相关的毒性提供了潜在的预防和干预策略。

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引用次数: 0

Corrigendum to “Honeybee colony soundscapes: Decoding distance-based cues and environmental stressors” [Ecotoxicol. Environ. Saf. 297 (2025) 118241] “蜂群声景：解码基于距离的线索和环境压力源”的勘误表[生态毒物]。环绕。[f].科学通报，2015(5):118241。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119486

Nayan Di, Chunjing Zhu, Zongwen Hu, Muhammad Zahid Sharif, Baizhong Yu

引用次数: 0

Levels of pesticide residues in the blood of ortolan buntings (Emberiza hortulana), skylarks (Alauda arvensis) and yellowhammers (Emberiza citrinella) from Sweden 来自瑞典的圃鹀、云雀和黄鹀血液中的农药残留水平。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119502

Sönke Eggers , Ove Jonsson , Matthew Low , Jan Sondell

Bird declines in European farmland have been linked to pesticide-driven food depletion and chronic exposure to pesticides well below levels causing acute toxicity. Yet, the extent of bird contamination by plant protection products remains largely unknown, partly because existing biomonitoring methods require relatively large blood volumes. Here, we developed a novel blood microsampling procedure (8 µl) combined with LC-MS/MS screening of 104 pesticides to determine exposure in nestling yellowhammers Emberiza citrinella (N = 5) and skylarks Alauda arvensis (N = 40) as well as adult ortolan buntings Emberiza hortulana (N = 21), species that have declined in European farmland over the last 60 years. Sampling was performed in Sweden from 2014 to 2016. Pesticide exposure was widespread: residues were detected in half of the nestlings (mean ± sd: 0.49 ± 0.07). Among individuals that tested positive for any pesticide, the mean number detected was 2.01 ± 0.49, with up to seven pesticides in a single nestling. Concentrations of individual pesticide residues in blood were relatively low compared to other studies (0.02–50 ng/ml) and were restricted to fungicides (N = 8) and herbicides (N = 5) in nestling skylarks and yellowhammers. Our results also provide the first evidence that exposure of migratory ortolan buntings to insecticide chlorpyrifos outside Sweden can be detected in the blood samples on their Swedish breeding grounds (7/10 tested positive; 0.25–0.56 ng/ml). These findings have key ecological and conservation implications, highlighting the need for and feasibility of, continued monitoring of pesticide exposure and its effects on non-target species in agro-ecosystems.

欧洲农田鸟类数量的减少与农药导致的食物消耗和长期暴露在远低于引起急性毒性水平的农药中有关。然而，植物保护产品对鸟类的污染程度在很大程度上仍然未知，部分原因是现有的生物监测方法需要相对较大的血容量。在这里，我们开发了一种新的血液微采样程序（8 µl），结合LC-MS/MS筛选104种农药，以确定雏鸟黄鹀Emberiza citrinella （N = 5）和云雀Alauda arvensis （N = 40）以及成年圃鹀Emberiza hortulana （N = 21）的暴露情况，这些物种在过去60年中在欧洲农田减少。2014年至2016年在瑞典进行了抽样。农药暴露很普遍：半数雏鸟体内检测到农药残留（平均值±sd: 0.49 ± 0.07）。在所有农药检测呈阳性的个体中，平均检测到的农药数量为2.01 ± 0.49，单个雏鸟最多可检测到7种农药。与其他研究相比，云雀和黄鹀血液中农药残留浓度相对较低（0.02 ~ 50 ng/ml），仅限于杀菌剂（N = 8）和除草剂（N = 5）。我们的研究结果还提供了第一个证据，证明在瑞典以外的地区，在瑞典繁殖地的血液样本中可以检测到迁徙的圃鹀接触杀虫剂毒死蜱（7/10检测呈阳性；0.25-0.56 ng/ml）。这些发现具有重要的生态和保护意义，突出了继续监测农药暴露及其对农业生态系统中非目标物种的影响的必要性和可行性。

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引用次数: 0

Exposure to environmental xenobiotics and lung tissue function: A comprehensive review on biological mechanisms and pathways 环境外源暴露与肺组织功能：生物学机制和途径的综合综述

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119438

Xiaojie Wu , Lin Lin , Hengyi Yan , Fushuang Zheng , Yuanyuan Ding , Huisheng Yao

Environmental xenobiotics, encompassing a wide spectrum of chemical pollutants such as particulate matter-bound polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), persistent organic pollutants (POPs), heavy metals, endocrine-disrupting chemicals (EDCs), pesticides, and emerging contaminants like nanomaterials and microplastics, have been increasingly implicated in impairing lung tissue function. These agents enter the body primarily through inhalation, particularly via outdoor air pollution, indoor contaminants, and occupational exposures, with additional contributions from ingestion and dermal absorption. Studies investigating these pollutants employ diverse exposure assessment methods, including environmental and biological monitoring, model-based estimations, and questionnaire-based tools. Lung function assessment spans from clinical spirometry and imaging to experimental histopathology and molecular biomarker analyses. Mechanistic evidence reveals that xenobiotics induce lung injury through oxidative stress, inflammation, mitochondrial dysfunction, epithelial barrier disruption, and epigenetic alterations. These processes lead to chronic respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, and lung cancer. Key signaling pathways implicated include activation of NF-κB, AP-1, and the aryl hydrocarbon receptor (AhR), promoting pro-inflammatory and cytotoxic responses. Furthermore, pollutant-induced epithelial permeability and fibrotic remodeling via TGF-β signaling exacerbate lung tissue damage and functional decline. While spirometry is widely used in population studies, it lacks sensitivity for early pathophysiological changes, necessitating integration with molecular and imaging approaches. Experimental models and in vitro studies provide valuable mechanistic insight, though challenges remain in translating findings to human populations. Current research underscores the complexity of real-world exposure scenarios and highlights the need for harmonized, multidisciplinary approaches combining environmental, biological, and molecular data. This comprehensive review synthesizes evidence across epidemiological and experimental studies, aiming to elucidate the biological pathways by which xenobiotic exposure compromises lung tissue function and to inform future research and regulatory strategies.

环境外来生物，包括广泛的化学污染物，如颗粒物质结合的多环芳烃（PAHs），挥发性有机化合物（VOCs），持久性有机污染物（POPs），重金属，内分泌干扰化学物质（EDCs），农药和新兴污染物，如纳米材料和微塑料，已经越来越多地涉及损害肺组织功能。这些物质主要通过吸入进入人体，特别是通过室外空气污染、室内污染物和职业接触，此外还有摄入和皮肤吸收。调查这些污染物的研究采用了多种暴露评估方法，包括环境和生物监测、基于模型的估计和基于问卷的工具。肺功能评估涵盖从临床肺活量测定和影像学到实验组织病理学和分子生物标志物分析。机制证据表明，外源药物通过氧化应激、炎症、线粒体功能障碍、上皮屏障破坏和表观遗传改变诱导肺损伤。这些过程导致慢性呼吸系统疾病，如哮喘、慢性阻塞性肺病（COPD）、肺纤维化和肺癌。涉及的关键信号通路包括NF-κB， AP-1和芳烃受体（AhR）的激活，促进促炎和细胞毒性反应。此外，污染物诱导的上皮通透性和通过TGF-β信号介导的纤维化重塑加剧了肺组织损伤和功能下降。虽然肺活量测定法广泛应用于人群研究，但它对早期病理生理变化缺乏敏感性，需要与分子和影像学方法相结合。实验模型和体外研究提供了有价值的机制见解，尽管在将研究结果转化为人类群体方面仍然存在挑战。目前的研究强调了现实世界暴露情景的复杂性，并强调需要统一的多学科方法，将环境、生物和分子数据结合起来。本综述综合了流行病学和实验研究的证据，旨在阐明外源性暴露损害肺组织功能的生物学途径，并为未来的研究和监管策略提供信息。

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引用次数: 0

Toxic effects of representative petroleum contaminants on earthworms (Eisenia fetida) under rainfall conditions 降雨条件下代表性石油污染物对蚯蚓的毒性影响

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119485

Zhifei Xiu , Na Zheng , Changcheng Chen , Qirui An , Zhiyuan Zhang , Qiuyan Lin , Xiaoqian Li , Liyuan Peng , Yunyang Li

During petroleum extraction and processing, petroleum contaminants readily pollute site soils. Under rainfall conditions, both contaminant migration and the activity of soil fauna are significantly affected. However, the implications of these interactions for soil fauna toxicity remain unclear. In this study, dodecane and naphthalene were selected as representative petroleum contaminants to investigate the migration pattern in site soils and their toxic effects on earthworms (Eisenia fetida), simulating interactions between soil surface contamination and different rainfall intensities (0.6 mm/d, 17.3 mm/d, and 60.2 mm/d). Both pollutants were primarily retained within the top 5 cm of soil; neither their vertical transport nor bioavailability significantly correlated with rainfall. Increasing rainfall intensity enhanced earthworm activity, thereby increasing their exposure to the pollutants, with the highest levels of 28.98 and 36.60 μg/g for dodecane and naphthalene, respectively. Accumulation of high concentrations of either contaminant damaged intestinal tissues and induced severe oxidative stress in earthworms. Dodecane and naphthalene exposure processes altered the composition of the earthworm gut flora and led to niche differentiation, regardless of rainfall intensity. The combined stress of contaminants and rainfall significantly disrupted earthworm metabolic pathways (energy, amino acids, carbohydrates, etc.). For hydrophobic organics like these, rainfall likely modulates toxicity risks to soil fauna not primarily through enhanced contaminant migration. Instead, it alters earthworm behavioral patterns, leading to differential contaminant exposure and subsequent toxicological responses.

在石油开采和加工过程中，石油污染物极易污染现场土壤。降雨条件下，污染物的迁移和土壤动物的活动都受到显著影响。然而，这些相互作用对土壤动物毒性的影响尚不清楚。本研究以十二烷和萘为代表的石油污染物，模拟不同降雨强度（0.6 mm/d、17.3 mm/d和60.2 mm/d）对土壤表面污染的影响，研究其在现场土壤中的迁移模式及其对蚯蚓的毒性作用。这两种污染物主要保留在土壤的前5 cm内；它们的垂直迁移和生物利用度与降雨均无显著相关。随着降雨强度的增加，蚯蚓的活动增强，从而增加了它们对污染物的暴露，其中十二烷和萘的最高水平分别为28.98和36.60 μg/g。高浓度的任何一种污染物的积累都会损害蚯蚓的肠道组织并引起严重的氧化应激。与降雨强度无关，十二烷和萘暴露过程改变了蚯蚓肠道菌群的组成并导致生态位分化。污染物和降雨的联合胁迫显著破坏了蚯蚓的代谢途径（能量、氨基酸、碳水化合物等）。对于像这样的疏水有机物，降雨可能会调节对土壤动物的毒性风险，而不是主要通过增强污染物迁移。相反，它改变了蚯蚓的行为模式，导致不同的污染物暴露和随后的毒理学反应。

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引用次数: 0

Multidimensional characterization of the groundwater contamination and the health risk in an industrial park: A combined multivariate statistics, source apportionment, and Monte Carlo simulation approach 工业园区地下水污染与健康风险的多维特征：多元统计、源解析和蒙特卡罗模拟相结合的方法。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119493

Li Meng , Yifei Wang , Rui Zuo , Linxi Xing , Zhigang Zhang , Xi Yang , Qiang Li , Jiawei Liu , Lingping Feng , Yidong Wang

Groundwater contamination at decommissioned petrochemical sites represents a significant understudied environmental challenge. This study addresses the urgent need for pollution source identification and risk assessment through an innovative framework integrating multivariate receptor modeling (PCA-APCS-MLR), multi-index pollution evaluation (PI/NPI/HPI/HEI), and probabilistic health risk analysis. Hydrochemical analysis for 201 samples (Oct-Dec 2024) revealed 47.39 % heavy metal enrichment (Mn > Fe > Cr > Zn > As) above background levels, with pollution indices confirming moderate-to-high contamination (HPI: 44.75–209.69; HEI: 5.03–42.44). Random forest regression identified Ca²⁺, Mg²⁺, ORP, TOC, and Na⁺ as primary control governing eight groundwater heavy metals. The strong agreement between measured and simulated values confirmed robust model predictability (R² = 0.703–0.799). PCA-APCS-MLR quantification identified five key sources: heavy metal pollution (16.42 %), industrial salts (13.73 %), petroleum hydrocarbons (14.51 %), water-rock interaction (14.44 %), and anthropogenic copper (11.33 %) (R² = 0.61–0.87). Health risks exhibited critical age-dependency: children showed higher non-carcinogenic risk (HI=13.70 vs. adults’ 7.27) while adults faced elevated carcinogenic risk (CR=2.54 ×10⁻³ vs. children’s 1.40 ×10⁻³). Over 99 % of total risk originated from oral ingestion, primarily driven by Cr and As exposure, with Monte Carlo simulations validating deterministic risk estimates. This integrated approach provides essential insights for source-specific remediation and exposure control in legacy industrial zones.

退役石化基地的地下水污染是一个尚未充分研究的重大环境挑战。本研究通过多变量受体模型（PCA-APCS-MLR）、多指标污染评价（PI/NPI/HPI/HEI）和概率健康风险分析相结合的创新框架，解决了污染源识别和风险评估的迫切需求。201份样品（2024年10月- 12月）的水化学分析结果显示，重金属（Mn > Fe > Cr > Zn > As）富集量高于背景水平47.39% %，污染指数为中高污染（HPI: 44.75 ~ 209.69; HEI: 5.03 ~ 42.44）。随机森林回归发现Ca 2 +、Mg 2 +、ORP、TOC和Na +是控制8种地下水重金属的主要控制方式。实测值和模拟值之间的高度一致性证实了模型的稳健可预测性（R²= 0.703-0.799）。PCA-APCS-MLR量化确定了5个主要污染源：重金属污染（16.42 %）、工业盐（13.73 %）、石油烃（14.51 %）、水岩相互作用（14.44 %）和人为铜（11.33 %）（R²= 0.61-0.87）。健康风险表现出严重的年龄依赖性：儿童表现出较高的非致癌风险（HI=13.70 vs.成人的7.27），而成人面临较高的致癌风险（CR=2.54 ×10对儿童的1.40 ×10⁻³）。超过99. %的总风险来自口服摄入，主要由铬和砷暴露引起，蒙特卡罗模拟验证了确定性风险估计。这种集成的方法为遗留工业区的特定源修复和暴露控制提供了必要的见解。

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引用次数: 0

Long-term individual and population effects of multiple stressors, using a model freshwater mollusc (Lymnaea stagnalis) 多种压力源对个体和种群的长期影响，以一种淡水软体动物为例。

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119505

Emily M. Moore , Iris Madge Pimentel , Ralf B. Schäfer , Claus Svendsen , Frances Orton

Human activities are driving biodiversity loss by increasing the exposure to multiple environmental stressors with pollution, climate change and invasive species being amongst the most important contributors. Here, we used the pond snail, Lymnaea stagnalis, as a model organism to investigate the combined effects of an environmentally relevant pollutant mixture, temperature increase and an invasive predator cue on physiological endpoints and behaviour. Dose-response data from single stressor exposures gathered over the partial life-cycle exposure period were used to calculate effect concentrations (EC10 and EC30) and responses to their combinations were investigated. At the EC30 for temperature (20 °C vs. 22 °C), effects were widely observed. While growth and reproduction were stimulated, this modest temperature increase negatively impacted survival (20 % reduction). Neither the pollutant mixture nor the predator cue severely impacted the examined responses and did not exacerbate the temperature effects. By contrast, the application of all three stressors at the EC30 level tended to ameliorate stress, compared to the temperature EC30 alone. Exploratory analyses also revealed that snails that avoided the predator cue by moving above the water line exhibited higher growth than those that remained in the water. Our results indicate that a number of organismal trade-offs may be occurring, such as between survival and reproduction, highlighting the complexities of predicting the impact of multiple stressors. Finally, the pronounced effects observed in response to this temperature increase (+2°C) is concerning, as this is within currently observed planetary warming, with organisms inhabiting small water bodies particularly susceptible due to their small water volumes.

人类活动增加了对多种环境压力源的暴露，导致生物多样性丧失，污染、气候变化和入侵物种是其中最重要的因素。本研究以塘螺（lynaea滞螺）为模型生物，研究了环境相关污染物混合物、温度升高和入侵捕食者线索对其生理终点和行为的综合影响。在部分生命周期暴露期间收集的单一应激源暴露的剂量-反应数据用于计算效应浓度（EC10和EC30），并研究了对其组合的反应。在EC30温度下（20°C vs. 22°C），效应被广泛观察到。在刺激生长和繁殖的同时，这种适度的温度升高对存活率产生了负面影响（降低20% %）。无论是污染物混合物还是捕食者线索都没有严重影响所检测的响应，也没有加剧温度效应。相比之下，与单独的EC30温度相比，在EC30水平下应用所有三种应激源倾向于改善应激。探索性分析还显示，通过在水线以上移动来避开捕食者提示的蜗牛比留在水中的蜗牛生长得更快。我们的研究结果表明，许多有机的权衡可能正在发生，例如在生存和繁殖之间，突出了预测多种压力源影响的复杂性。最后，对温度升高（+2°C）所观察到的显著影响令人担忧，因为这是在目前观测到的全球变暖范围内，由于水量小，生活在小水体中的生物特别容易受到影响。

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引用次数: 0

Development, validation and application of ERα- and PPARγ-mediated female reproductive toxicity prediction models based on adverse outcome pathway 基于不良结局通路的ERα-和ppar γ-介导的女性生殖毒性预测模型的建立、验证和应用

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety

Pub Date : 2025-12-01 DOI: 10.1016/j.ecoenv.2025.119503

Ya Tian, Liang Lyu, Hui-ming Yang, Yi Yang, Zheng Li, Yi-yuan Zeng, Hong-zhan Liu, Li-li Yang, Xiao-feng Zhang

Traditional methods for reproductive toxicity assessment are insufficient for high-throughput chemical screening. Although efforts have been made in recent years to develop predictive models for reproductive toxicity, in-depth modeling of female-specific multi-pathway mechanisms has been lacking. In this study, we developed a female reproductive toxicity prediction model, FeRetoxML, based on adverse outcome pathway (AOP) mediated by estrogen receptor α (ERα) and peroxisome proliferator-activated receptor γ (PPARγ), employing a closed-loop strategy of model development, validation and application, which is the first model established to link PPARγ pathway with rat estrous cycle changes to predict ovarian toxicity. Evaluation on additional datasets showed that the accuracy of FeRetoxML for female reproductive toxicity mediated by the ERα and PPARγ pathways was 84.6 % and 83.3 %, respectively. Also, this study applied the BiLSTM algorithm to construct FeRetoxBiLSTM, which, despite lower predictive performance, exhibited notable application potential. Our study introduced a novel AOP-based modeling approach for high-throughput screening of female reproductive toxicants, and this approach may be applicable to other toxicological endpoints. The models can accurately identify the chemicals with female reproductive toxicity, and provide a promising tool for chemical management, including determining the priority order of in vivo reproductive toxicity testing for chemicals.

传统的生殖毒性评估方法不足以进行高通量化学筛选。尽管近年来已经努力开发生殖毒性的预测模型，但缺乏对女性特异性多途径机制的深入建模。本研究基于雌激素受体α (ERα)和过氧化物酶体增殖物激活受体γ (PPARγ)介导的不良结局通路（AOP），采用模型开发、验证和应用的闭环策略，建立了雌性生殖毒性预测模型FeRetoxML，这是第一个将PPARγ通路与大鼠发色周期变化联系起来预测卵巢毒性的模型。对其他数据集的评估表明，FeRetoxML对ERα和PPARγ途径介导的雌性生殖毒性的准确性分别为84.6 %和83.3 %。此外，本研究采用BiLSTM算法构建了FeRetoxBiLSTM，尽管预测性能较低，但显示出显著的应用潜力。我们的研究介绍了一种新的基于aop的建模方法，用于女性生殖毒物的高通量筛选，这种方法可能适用于其他毒理学终点。该模型可以准确识别具有雌性生殖毒性的化学物质，为化学物质管理提供了一种有前景的工具，包括确定化学物质体内生殖毒性测试的优先顺序。

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引用次数: 0