Ecotoxicology and Environmental Safety最新文献

{"title":"Molecular characterization of Pleiotropic Drug Resistance (PDR) genes involved in tolerance of cadmium in peanut (Arachis hypogaea L.)","authors":"Qi Wang ,&nbsp;Xiaoxu Li ,&nbsp;Zhiyuan Li ,&nbsp;Quanxi Sun ,&nbsp;Chunjuan Li ,&nbsp;Xiaobo Zhao ,&nbsp;Shihua Shan","doi":"10.1016/j.ecoenv.2024.117324","DOIUrl":"10.1016/j.ecoenv.2024.117324","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) is one of the most important oil crops worldwide. Cadmium (Cd), a heavy metal that is nonessential and toxic, has the potential to significantly impacted the quality and safety of peanut. Despite the known importance of Pleiotropic Drug Resistance (PDR) genes in heavy metal accumulation and transport in plants, there is a lack of comprehensive research on the systematic identification and functional characterization of AhPDRs in peanut. In this study, a total of 38 <em>AhPDR</em> genes were discovered within the peanut genome. Among these, <em>AhPDR24</em>, <em>AhPDR30</em>, and <em>AhPDR33</em> displayed notable variations in expression levels in response to Cd stress. Particularly noteworthy was the observation that <em>AhPDR33</em>, localized in the plasma membrane, exhibited a significant increase in expression (approximately 3.8-fold) and heightened promoter activity (approximately 4.1-fold) following exposure to Cd (75 μM CdCl2). Furthermore, the study found that the overexpression of <em>AhPDR33</em> in <em>Arabidopsis</em> resulted in increased root elongation and decreased Cd accumulation (approximately 0.42-fold) compared to wild-type plants. This suggests that <em>AhPDR33</em> may have a beneficial role in facilitating Cd efflux and tolerance in plants. Additionally, transient silencing of <em>AhPDR33</em> in peanut demonstrated its positive regulation of Cd tolerance through the promotion of reactive oxygen species (ROS) scavenging and membrane permeability reduction. These findings contribute to the understanding of the molecular mechanisms involved in <em>AhPDR33</em>-mediated Cd tolerance and detoxification in peanut. Furthermore, this study provides comprehensive information to understand the <em>AhPDR</em> gene family, its features, and its expression, which will hold a promising utility as an excellent candidate in the genetic improvement of peanut Cd stress tolerance.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117324"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speciation characteristics of heavy metal(loid)s in maize-wheat farmland with applying spent mushroom substrates","authors":"Ludan Chen ,&nbsp;Wei Zhou ,&nbsp;Yuhai Bao ,&nbsp;Xiubin He ,&nbsp;Liangji Deng","doi":"10.1016/j.ecoenv.2024.117329","DOIUrl":"10.1016/j.ecoenv.2024.117329","url":null,"abstract":"<div><div>Spent mushroom substrates (SMS) have been increasingly applied as organic fertilizer worldwide. However, the effects of various SMS on the accumulation and speciation characteristics of soil heavy metal(loid)s (HMs) are generally overlooked. Three types of SMS, including <em>Flammulina velutipes</em> residue (FVR), <em>Agaricus bisporus</em> residue (ABR), and <em>Auricularia auricula</em> residue (AAR), were applied to replace 25 % and 50 % of chemical fertilizer (based on nitrogen application) used in maize-wheat farmland. Compared to chemical fertilizer, the soil Cd, Pb, and As concentrations were decreased by 20.41 %, 5.97 %, and 10.09 %, respectively. And the residual fractions of soil HMs were increased through the application of SMS, indicating a reduction in their bioavailability. Notably, 50 % ABR replacement significantly increased the proportion of residual fraction in soil Cd, Pb, and As by 23.03 %, 15.15 %, and 4.85 %, respectively (<em>P</em>&lt;0.05). A significant negative correlation was observed between the concentrations of HMs in grains and the residual fractions of soil HMs. Thus, compared with chemical fertilizers, the residual fractions of soil HMs were increased by the application of SMS, thereby reducing the concentrations of HMs in grains. Ingestion of crops is the primary route for human exposure to HMs. Therefore, the application of SMS (especially ABR) reduced the accumulation and bioavailability of HMs in soil, which in turn limited the transfer of HMs to crops, resulting in lowered human health risk indices.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"288 ","pages":"Article 117329"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and physiological effects of antibiotic-induced dysbiosis in citrus","authors":"Toi Ketehouli ,&nbsp;Erica M. Goss ,&nbsp;Marina S. Ascunce ,&nbsp;Samuel J. Martins","doi":"10.1016/j.ecoenv.2024.117325","DOIUrl":"10.1016/j.ecoenv.2024.117325","url":null,"abstract":"<div><div>Streptomycin (Str) and oxytetracycline (Otc) are widely used antibiotics to manage bacterial diseases in citrus and other crops. However, their impacts on the rhizosphere bacterial assembly and plant physiology are poorly understood. The aim of this study was to examine the effects of Str and Otc on the physiology (assimilation, transpiration rate, intracellular CO₂, and stomatal conductance to water vapor), rhizosphere bacterial assemblages (16S rRNA gene high-throughput amplicon sequencing), and rhizosphere metabolite profiles in healthy <em>Citrus reticulata</em> trees. The results indicated a reduction in photosynthesis after Str and Otc treatments, whereas CO₂ outflow stayed constant. Both antibiotics decreased the culturable numbers of bacteria. Analysis of the microbiome showed changes in relative abundance of bacterial groups, specifically <em>Pseudomonas, Agrobacterium,</em> and <em>Streptomyces</em>, in response to the antibiotics. Metabolite profiles changed in streptomycin- and oxytetracycline-treated citrus plants suggesting response to microbe targets or induction of stress responses. This study advances knowledge of antibiotic-driven effects on the rhizosphere microbiome, rhizosphere metabolome, and plant physiology, which is essential for managing plant diseases while safeguarding rhizosphere ecology and long-term plant health.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117325"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics in indoor and outdoor environments in China: Characteristic and human exposure risk assessment","authors":"Han Zheng,&nbsp;Huibin Guo,&nbsp;Haiyan Fu,&nbsp;Kaixing Yao","doi":"10.1016/j.ecoenv.2024.117328","DOIUrl":"10.1016/j.ecoenv.2024.117328","url":null,"abstract":"<div><div>Human exposure to microplastics (MPs) has led to global health concerns, but our knowledge of the characteristics and human exposure of airborne MPs is limited. Adults may have different exposure patterns and lifestyles from other age groups. Therefore, this study aims to determine the MPs exposure among adults at various locations and during different time periods. MPs were collected and detected through passive sampling and laser direct infrared (LDIR) imaging (Agilent 8700) at five locations including: dormitory, dining hall, office, library, and outdoor; the sampling lasted for 3 months. The highest concentration of indoor MPs was detected in the dining hall (193 ± 8 MPs/m²/day), whereas the lowest was detected in the library (113 ± 4 MPs/m²/day). Among all sampling points, the outdoor locations had the lowest MPs concentrations (92 ± 4 MPs/m²/day). The length of the MPs ranged from 10 μm－760 μm. Pellets (54.6 %), fibres (21.6 %), and fragments (23.8 %) were the shapes identified in this research. Polyamide (51.7 %) was the most prevalent polymer type at all sampling points. This article conducted respiratory exposure assessments of MPs for males and females of different ages (age ranges: 18－21; 21－30; 31－40; 41－60) in different environments and at different times. For both weekends and weekdays, dormitories contributed the most to MPs respiratory exposure. Males aged 31－40 years had the highest exposure with an average of 266 particles/day, whereas females aged 18－21 (157 particles/day) had the lowest exposure. Differences in respiratory rate according to age and gender may be the main reason for these results. These findings indicate that further research into the adult MPs inhalation exposure under indoor and outdoor conditions is crucial.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117328"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional effects of nitrification and urease inhibitors: Decreasing soil herbicide residues and reducing nitrous oxide emissions simultaneously","authors":"Yaohui Liu ,&nbsp;Weijin Wang ,&nbsp;Manyun Zhang ,&nbsp;Negar Omidvar ,&nbsp;Haoqi Fan ,&nbsp;Kewei Ren ,&nbsp;Wenyuan Zhang ,&nbsp;Dongnan Hu ,&nbsp;Yihua Xiao","doi":"10.1016/j.ecoenv.2024.117301","DOIUrl":"10.1016/j.ecoenv.2024.117301","url":null,"abstract":"<div><div>Glyphosate pollution and greenhouse gas emissions are major problem in achieving sustainable soil management. It is necessary to develop effective strategies to simultaneously reduce herbicide residues and nitrous oxide (N₂O) emissions in soil. This study aimed to: (1) quantitative analyze the effects of nitrogen (N) cycle inhibitors (nitrification inhibitors 3,4 dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) and urease inhibitor N-(<em>n</em>-butyl) thiophosphoric triamide (NBPT)) on glyphosate degradation and reduction of N₂O under different soil moistures; (2) identify the functional microbes and genes associated with glyphosate degradation and N₂O emissions; and (3) decipher the main mechanisms of N cycle inhibitors affecting glyphosate degradation at different soil water contents. Compared to the control, the application of DMPP, DCD and NBPT reduced glyphosate residues in soil by 33.0 %, 60.3 % and 35.7 %, respectively, under 90 % water holding capacity (WHC). The application of DCD stimulated <em>Acidobacteria</em> and the <em>phn</em>X gene to degrade soil glyphosate. Further, soil glyphosate residues were significantly and negatively related to soil N₂O emissions at both 60 % and 90 % WHC. Compared to the control, NBPT application decreased cumulative N₂O emissions by 91.4 % at 90 % WHC by decreasing soil nitrate N (NO₃^--N) and inhibiting <em>amo</em>C and <em>nar</em>G genes at 90 %. The application of N cycle inhibitors could be a potential strategy to simultaneously reduce glyphosate residues and soil N₂O emissions. Our study could provide technical support to reduce the risks of herbicide exposure and reduce greenhouse gas emissions.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117301"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals decreased expression of detoxification genes in Caenorhabditis elegans exposed to mepanipyrim and cyprodinil: Implications for multigenerational exposure effects","authors":"Tingting Gai ,&nbsp;Lianfeng Zhang ,&nbsp;Liangwen Chen ,&nbsp;Yun Wang ,&nbsp;Xiaonan Sun ,&nbsp;Yuhao Yang ,&nbsp;Wenjing Cao ,&nbsp;Yanyan Chen","doi":"10.1016/j.ecoenv.2024.117265","DOIUrl":"10.1016/j.ecoenv.2024.117265","url":null,"abstract":"<div><div>The broad-spectrum fungicides mepanipyrim (Mep) and cyprodinil (Cyp) have been reported to be used worldwide to control gray mold of fruit crops. Consequently, they are often detected in the water and food items. However, the impacts and potential mechanisms of these two pesticides on environmental organisms remain unclear. Utilizing <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) as the model, the toxic effects and mechanisms were analyzed after Mep and Cyp exposure over four generations (P₀-F₃). The results showed that Mep and Cyp at concentrations over 0.1 µg/L affected the motility behavior, while 1.0 µg/L dosage and above inhibited the growth of <em>C. elegans</em>. In addition, multigenerational exposure could significantly impair both locomotion and development of <em>C. elegans</em> even at 0.01 µg/L dosage. Notably, constant exposure resulted in the function abnormalities of cholinergic, dopaminergic and GABAergic neurons, as well as the accumulation of ROS. Further transcriptome analysis showed reduced expression of 14 genes and 26 genes in xenobiotic metabolic pathway following Mep and Cyp exposure, respectively. The buildup of Mep/Cyp and ROS might have led to nerve impairment and behavioral abnormality. These findings enhanced understanding of the toxic effects of Mep and Cyp and provided insights into their eco-toxicological evaluation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117265"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of exposure to phthalate with serum uric acid and hyperuricemia risk, and the mediating role of systemic immune inflammation","authors":"Zhiping Niu ,&nbsp;Tianyi Chen ,&nbsp;Zhizhou Duan ,&nbsp;Shichao Han ,&nbsp;Yifan Shi ,&nbsp;Wenyuan Yu ,&nbsp;Shuang Du ,&nbsp;Hao Tang ,&nbsp;Wenpu Shao ,&nbsp;Jin Sun ,&nbsp;Han Chen ,&nbsp;Yunfei Cai ,&nbsp;Yanyi Xu ,&nbsp;Zhuohui Zhao","doi":"10.1016/j.ecoenv.2024.117269","DOIUrl":"10.1016/j.ecoenv.2024.117269","url":null,"abstract":"<div><h3>Background</h3><div>Previous studies found that urinary phthalates (PAEs) metabolites may be associated with increased serum uric acid concentration and hyperuricemia risk. However, no population-based study has investigated the underlying biological mechanisms.</div></div><div><h3>Methods</h3><div>This nationwide cross-sectional study analyzed the data from the National Health and Nutrition Examination Survey (NHANES) 2003–2018. Urinary PAEs metabolites were measured and 8 PAEs metabolites (MCPP, MECPP, MEHHP, MEOHP, MBzP, MiBP, MBP, and MEP) were incorporated into the analysis. Serum uric acid was determined and hyperuricemia cases were identified. Multi-variable generalized linear model, exposure-response (E-R) function and weighted quantile sum (WQS) regression were utilized to investigate the relationships of PAEs metabolites with serum uric acid concentration and hyperuricemia risk. Systemic immune inflammation (SII) was assessed using the SII index and its mediation effects were explored using causal mediation effect model.</div></div><div><h3>Results</h3><div>Data from 10,633 US adults in the NHANES 2003–2018 was analyzed. Except for MEP, individual PAEs metabolite and total PAEs metabolites were associated with increased serum uric acid concentration and hyperuricemia risk. E-R function of PAEs metabolites with serum uric acid concentration and the risk of hyperuricemia showed significantly positive associations with most curves in a nearly linear relationship. WQS regression showed that the mixture of PAEs metabolites was related to elevated serum uric acid and hyperuricemia risk, and MBzP was identified as the most contributing PAEs metabolite. The causal mediation effect model found that SII significantly mediated the relationships of PAEs metabolites with serum uric acid and hyperuricemia risk.</div></div><div><h3>Conclusion</h3><div>Individual and mixture of urinary PAEs metabolites were associated with increased serum uric acid concentration and the risk of hyperuricemia. MBzP exhibited the highest contribution to the overall effects. SII alteration may be an important biological mechanism underlining the impact of PAEs metabolites on serum uric acid concentration and hyperuricemia risk.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117269"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and renal protective effects of Nano-selenium on adenine-induced acute renal failure in canines","authors":"Mengdi Zhang ,&nbsp;Jindong Gao ,&nbsp;Md. F. Kulyar ,&nbsp;Wanhe Luo ,&nbsp;Guodong Zhang ,&nbsp;Xiaoqi Yang ,&nbsp;Tianguang Zhang ,&nbsp;Haihang Gao ,&nbsp;Yuxuan Peng ,&nbsp;Jiabin Zhang ,&nbsp;Muhammad Altaf ,&nbsp;Samah Attia Algharib ,&nbsp;Donghai Zhou ,&nbsp;Jianzhong He","doi":"10.1016/j.ecoenv.2024.117274","DOIUrl":"10.1016/j.ecoenv.2024.117274","url":null,"abstract":"<div><div>Acute renal failure is a common clinical disease in canines, affecting antioxidant levels and decreasing the body's resistance. This study aims to explore the therapeutic mechanism of Nano-selenium in acute renal failure. The histopathological and imaging changes of kidney tissue were observed with the gene and protein expression levels of Keap1, Nrf2, HO-1, and NQO1 in the kidney. According to our findings, adding nano-selenium can effectively reduce the concentration of CRE and BUN in blood and kidney tissues. It increased the activity of GSH-PX and SOD by an effective reduction of MDA. Through pathological and imaging observations, it was found that adding nano-selenium could improve the kidney tissue structure of acute renal failure. The results of the RT-qPCR experiment showed that after the addition of nano-selenium, the mRNA expression of the Keap1 gene decreased significantly. In contrast, the mRNA expression of the Nrf2, HO-1, and NQO1 genes increased significantly. The experimental results were further verified by western blot and immunohistochemical analysis. Hence, the nano-selenium intervention improved kidney function and increased antioxidant levels in canines suffering from acute renal failure with the involvement of the Keap1-Nrf2/ARE signaling pathway.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117274"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between blue space exposure and rheumatoid arthritis: The modifying effect of genetic susceptibility and air pollutants","authors":"Yi-Sheng He ,&nbsp;Man Ge ,&nbsp;Yi-Qing Xu ,&nbsp;Zhao-Xing Gao ,&nbsp;Tian He ,&nbsp;Peng Zhang ,&nbsp;Sha-Sha Tao ,&nbsp;Peng Wang ,&nbsp;Zhu Chen ,&nbsp;Hai-Feng Pan","doi":"10.1016/j.ecoenv.2024.117346","DOIUrl":"10.1016/j.ecoenv.2024.117346","url":null,"abstract":"<div><div>Studies on the interaction among genetic susceptibility, blue space exposure, and rheumatoid arthritis (RA) risk have been lacking. Therefore, we examined the association between blue space exposure and RA incidence and assess the modifying effect of genetic susceptibility and air pollutants. Form the UK Biobank, 322,783 participants without RA were enrolled in this study. The association between blue space exposure and RA incidence was estimated using a cox proportional hazards model. The combined effect of blue space and genetic factors on the risk of RA was further evaluated. The polygenic risk score (PRS) for RA was calculated to represent individual genetic risk, and the potential modification effect of air pollution on the relationship between blue space, PRS, and RA were explored. During a median follow-up of 12.4 years, 3659 RA cases were identified. A 10 % increase in blue space_300 m was associated with a 22.6 % reduction in RA incidence (HR=0.774, 95 % CI: 0.670, 0.895), exhibiting a consistent downward trend in the exposure-response curve. A high PRS was an independent risk factor for RA (HR=1.393, 95 % CI: 1.347, 1.439). The associations between blue space exposure, PRS, and the risk of RA were dose-dependent, with the lowest risk observed among those with high levels of blue space and lower PRS (HR_{bluespace300m}=0.501, 95 % CI: 0.431, 0.583; HR_{bluespace1000m}=0.476, 95 % CI: 0.408, 0.555). Interaction analysis indicated that increased concentrations of air pollutants strengthened the relationship between PRS and RA. Blue space exposure mitigated the risk of RA development, particularly in individuals with low genetic risk.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117346"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of adverse outcome pathway, potency, human exposure supports carcinogenicity of polyhexamethylene guanidine phosphate in lung cancer","authors":"Yong Joo Park ,&nbsp;Ha Ryong Kim ,&nbsp;Jun Woo Kim ,&nbsp;Jong-Hyun Lee ,&nbsp;Younghee Kim ,&nbsp;Jungyun Lim ,&nbsp;Yong-Wook Baek ,&nbsp;Kyu Hyuck Chung","doi":"10.1016/j.ecoenv.2024.117222","DOIUrl":"10.1016/j.ecoenv.2024.117222","url":null,"abstract":"<div><div>In this study, we investigated the potential mechanisms by which polyhexamethylene guanidine phosphate (PHMG-p), a known respiratory irritant, may contribute to lung cancer development. Using the adverse outcome pathway (AOP) framework, we analyzed established databases (such as AOP-Wiki) and employed AI tools (AOP-helpFinder) to identify key events (KEs) associated with lung carcinogenesis. Our analysis indicates that chronic inhalation of PHMG-p triggers a non-genotoxic pathway, characterized by cell membrane disruption, inflammation, and oxidative stress, with a point of departure (POD) of 0.0018 mg/m³, suggesting carcinogenic potential. Additionally, a human exposure assessment revealed that most claimants were exposed to PHMG-p levels exceeding the estimated inhalation reference concentration (RfC) of 0.018 µg/m³. While downstream KEs, such as DNA damage, mutation, and cell proliferation, require further investigation, our findings, supported by the AOP framework and potency and exposure assessments, strongly suggest that PHMG-p exposure could induce lung cancer in individuals affected by humidifier disinfectants. These results underscore the importance of a comprehensive risk assessment approach for evaluating the carcinogenicity of PHMG-p.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"287 ","pages":"Article 117222"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}