Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117084
Antimony (Sb) is a metalloid, widely presents in the environment and associates with human health. In this study, we aimed to decipher whether Sb exposure is harmful to female reproduction and explore the underlying mechanisms. The ICR mice were exposed to 0, 5, 10, and 20 mg/kg acetate potassium Sb tartrate trihydrate by intraperitoneal injection for 10 days, then mouse oocytes were collected for further analysis. We first found a significant decrease in the proportion of MII-stage oocytes obtained from supernumerary ovulation in the fallopian tubes and early embryo development under Sb treatment. Then a series of tests showed Sb affects oocyte maturation by damaging the cytoskeleton of microtubule and actin. Moreover, the abnormal distribution of cortical granules and their component Ovastacin in oocytes, combined with reduced expression levels of Juno, affected sperm-oocyte binding and led to fertilization failure. Based on the sequencing results and experimental validation, it was demonstrated that Sb exposure impairs mitochondrial distribution and membrane potential, elevated levels of mitochondrial superoxide, finally caused energy supply deficits. Mitochondrial damage in oocytes after Sb exposure results in the excessive oxidative stress and early apoptosis. Taken together, these data suggest that Sb exposure decreases oocyte quality and female fertilization ability by impairing mitochondrial function and redox perturbation.
{"title":"Antimony exposure affects oocyte quality and early embryo development via excessive mitochondrial oxidation and dysfunction","authors":"","doi":"10.1016/j.ecoenv.2024.117084","DOIUrl":"10.1016/j.ecoenv.2024.117084","url":null,"abstract":"<div><div>Antimony (Sb) is a metalloid, widely presents in the environment and associates with human health. In this study, we aimed to decipher whether Sb exposure is harmful to female reproduction and explore the underlying mechanisms. The ICR mice were exposed to 0, 5, 10, and 20 mg/kg acetate potassium Sb tartrate trihydrate by intraperitoneal injection for 10 days, then mouse oocytes were collected for further analysis. We first found a significant decrease in the proportion of MII-stage oocytes obtained from supernumerary ovulation in the fallopian tubes and early embryo development under Sb treatment. Then a series of tests showed Sb affects oocyte maturation by damaging the cytoskeleton of microtubule and actin. Moreover, the abnormal distribution of cortical granules and their component Ovastacin in oocytes, combined with reduced expression levels of Juno, affected sperm-oocyte binding and led to fertilization failure. Based on the sequencing results and experimental validation, it was demonstrated that Sb exposure impairs mitochondrial distribution and membrane potential, elevated levels of mitochondrial superoxide, finally caused energy supply deficits. Mitochondrial damage in oocytes after Sb exposure results in the excessive oxidative stress and early apoptosis. Taken together, these data suggest that Sb exposure decreases oocyte quality and female fertilization ability by impairing mitochondrial function and redox perturbation.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011606/pdfft?md5=09f62a72d6c9f73dc45a99c3fb8c44f1&pid=1-s2.0-S0147651324011606-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117077
Farmland, a vital terrestrial environment, has seen significant changes due to intensified agricultural practices and plastic pollution. Assessing plastic pollution in farmed areas remains challenging, and understanding its impacts on agricultural ecosystems is limited. The aim of the was to determine whether plastic pollution in agricultural landscapes depends on the type of crops. We used the Red-backed Shrike (Lanius collurio), an indicator bird species and investigated plastic contamination of 122 of its nests. We found plastic waste in 82.1 % of nests, totalling 6234 items weighing 197.2 g. On average, each nest contained 55.7 waste items, constituting approximately 4.3 % of the nest's mass. Plastic string fibres were predominant, making up 95.6 % of the items and 90.7 % of the mass. This variation may be due to different straw and hay management practices and varying levels of vigilance in removing plastic remnants. Contamination levels varied significantly among farmland types, with cultivated fields showing the highest contamination and meadows the lowest.
{"title":"Assessing plastic pollution in farmland: Impact of agricultural practices on bird nesting materials","authors":"","doi":"10.1016/j.ecoenv.2024.117077","DOIUrl":"10.1016/j.ecoenv.2024.117077","url":null,"abstract":"<div><div>Farmland, a vital terrestrial environment, has seen significant changes due to intensified agricultural practices and plastic pollution. Assessing plastic pollution in farmed areas remains challenging, and understanding its impacts on agricultural ecosystems is limited. The aim of the was to determine whether plastic pollution in agricultural landscapes depends on the type of crops. We used the Red-backed Shrike (<em>Lanius collurio</em>), an indicator bird species and investigated plastic contamination of 122 of its nests. We found plastic waste in 82.1 % of nests, totalling 6234 items weighing 197.2 g. On average, each nest contained 55.7 waste items, constituting approximately 4.3 % of the nest's mass. Plastic string fibres were predominant, making up 95.6 % of the items and 90.7 % of the mass. This variation may be due to different straw and hay management practices and varying levels of vigilance in removing plastic remnants. Contamination levels varied significantly among farmland types, with cultivated fields showing the highest contamination and meadows the lowest.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011539/pdfft?md5=0f60ad95ff69a7588867aa4f1f2efe67&pid=1-s2.0-S0147651324011539-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117054
Extensive research has established the link between PM2.5 exposure and blood pressure (BP) levels among normal individuals. However, the association between PM2.5 components and BP levels in hypertensive patients has not been fully explored. In this study, 12 971 hypertensive cases from Jinchang cohort (in Jinchang City, China) with nearly 9 years of follow-up were enrolled. Based on the linear mixed-effect model, the effects of fine particulate matter (PM2.5) and five major components [sulfate (SO42-), nitrate (NO3-), ammonium (NH4+), black carbon (BC) and organic matter (OM)]on BP [systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and pulse pressure (PP)]were evaluated by single-component model, component-joint model and component-residual model, respectively. A positive correlation was found between PM2.5 as well as its components (SO42-, NO3-, NH4+, BC and OM) exposure and BP levels. The effects of SO42-, BC and OM on BP were observed to be the most robust among the three models. Based on the results of interaction effects and stratified analysis, the effect of BC exposure on SBP, and the effect of PM2.5 and its five components on PP were greater in female than in males. Compared with elderly hypertensive patients, OM had more significant effects on SBP, DBP and MAP in young and (or) middle-aged hypertensive patients. During the heating season, the effect of PM2.5 and its components on BP was grater compared to the non-heating season. Meanwhile, PM2.5 and its components have a greater influence on BP in patients with hypertension combined with diabetes. Therefore, the findings suggested that both PM2.5 exposure and its components had a significant effect on BP in patients with hypertension. Women and young and middle-aged hypertensive patient were the sensitive population. The implementation of source control and reduction of PM2.5 emission (mainly for SO42-, BC and OM) may be of great significance to control BP level and could reduce the risk of cardiovascular disease in patients with hypertension.
{"title":"A longitudinal study on the effect of PM2.5 components on blood pressure in the hypertensive patients from 2011 to 2019","authors":"","doi":"10.1016/j.ecoenv.2024.117054","DOIUrl":"10.1016/j.ecoenv.2024.117054","url":null,"abstract":"<div><div>Extensive research has established the link between PM<sub>2.5</sub> exposure and blood pressure (BP) levels among normal individuals. However, the association between PM<sub>2.5</sub> components and BP levels in hypertensive patients has not been fully explored. In this study, 12 971 hypertensive cases from Jinchang cohort (in Jinchang City, China) with nearly 9 years of follow-up were enrolled. Based on the linear mixed-effect model, the effects of fine particulate matter (PM<sub>2.5</sub>) and five major components [sulfate (SO<sub>4</sub><sup>2-</sup>), nitrate (NO<sub>3</sub><sup>-</sup>), ammonium (NH<sub>4</sub><sup>+</sup>), black carbon (BC) and organic matter (OM)]on BP [systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and pulse pressure (PP)]were evaluated by single-component model, component-joint model and component-residual model, respectively. A positive correlation was found between PM<sub>2.5</sub> as well as its components (SO<sub>4</sub><sup>2-</sup>, NO<sub>3</sub><sup>-</sup>, NH<sub>4</sub><sup>+</sup>, BC and OM) exposure and BP levels. The effects of SO<sub>4</sub><sup>2-</sup>, BC and OM on BP were observed to be the most robust among the three models. Based on the results of interaction effects and stratified analysis, the effect of BC exposure on SBP, and the effect of PM<sub>2.5</sub> and its five components on PP were greater in female than in males. Compared with elderly hypertensive patients, OM had more significant effects on SBP, DBP and MAP in young and (or) middle-aged hypertensive patients. During the heating season, the effect of PM<sub>2.5</sub> and its components on BP was grater compared to the non-heating season. Meanwhile, PM<sub>2.5</sub> and its components have a greater influence on BP in patients with hypertension combined with diabetes. Therefore, the findings suggested that both PM<sub>2.5</sub> exposure and its components had a significant effect on BP in patients with hypertension. Women and young and middle-aged hypertensive patient were the sensitive population. The implementation of source control and reduction of PM<sub>2.5</sub> emission (mainly for SO<sub>4</sub><sup>2-</sup>, BC and OM) may be of great significance to control BP level and could reduce the risk of cardiovascular disease in patients with hypertension.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011308/pdfft?md5=6372db13205b0448344300aab0f95fe1&pid=1-s2.0-S0147651324011308-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117079
River pollution can harm human health through direct contact, drinking water, and the consumption of contaminated fish and irrigated agricultural products. Surface water and Nile tilapia (Oreochromis niloticus) samples were collected monthly from July 2022 to June 2023 at three sites (El-Rahawy, Sabal, and Tala) along the Rosetta Nile branch in Egypt to monitor the presence of eight trace elements. The potential human health risks from consuming contaminated fish were also assessed. Iron and manganese were consistently detected in all water samples across most seasons and locations, with concentrations generally below the WHO permissible levels. All 72 analyzed fish muscle samples were found to contain trace elements. The mean concentrations of metals in the fish muscle samples, in descending order, were: iron > zinc > copper > manganese > tin > antimony > lead > mercury. Significant spatial and seasonal variations were observed in both water and fish samples. El-Rahawy was identified as the most contaminated site, with summer exhibiting the highest contamination rate compared to other seasons. Fish samples collected from El-Rahawy demonstrated the highest bioconcentration factor (BCF) values for most elements, particularly mercury, lead, iron, manganese, and antimony. Target hazard quotient (THQ) calculations for the trace elements in Nile tilapia muscles revealed that all trace elements, except antimony, had THQ values below 1, suggesting that consuming Nile tilapia from these sites is unlikely to cause adverse health effects. However, THQ values for antimony exceeded the threshold of 1, indicating a potential health risk for consumers. Although the detected trace elements in the fish were below the permissible toxicity limits, some could pose a future threat to human health, necessitating further studies, ongoing monitoring, and preventive measures.
{"title":"Assessment of trace element occurrence in Nile Tilapia from the Rosetta branch of the River Nile, Egypt: Implications for human health risk via lifetime consumption","authors":"","doi":"10.1016/j.ecoenv.2024.117079","DOIUrl":"10.1016/j.ecoenv.2024.117079","url":null,"abstract":"<div><div>River pollution can harm human health through direct contact, drinking water, and the consumption of contaminated fish and irrigated agricultural products. Surface water and Nile tilapia (<em>Oreochromis niloticus</em>) samples were collected monthly from July 2022 to June 2023 at three sites (El-Rahawy, Sabal, and Tala) along the Rosetta Nile branch in Egypt to monitor the presence of eight trace elements. The potential human health risks from consuming contaminated fish were also assessed. Iron and manganese were consistently detected in all water samples across most seasons and locations, with concentrations generally below the WHO permissible levels. All 72 analyzed fish muscle samples were found to contain trace elements. The mean concentrations of metals in the fish muscle samples, in descending order, were: iron > zinc > copper > manganese > tin > antimony > lead > mercury. Significant spatial and seasonal variations were observed in both water and fish samples. El-Rahawy was identified as the most contaminated site, with summer exhibiting the highest contamination rate compared to other seasons. Fish samples collected from El-Rahawy demonstrated the highest bioconcentration factor (BCF) values for most elements, particularly mercury, lead, iron, manganese, and antimony. Target hazard quotient (THQ) calculations for the trace elements in Nile tilapia muscles revealed that all trace elements, except antimony, had THQ values below 1, suggesting that consuming Nile tilapia from these sites is unlikely to cause adverse health effects. However, THQ values for antimony exceeded the threshold of 1, indicating a potential health risk for consumers. Although the detected trace elements in the fish were below the permissible toxicity limits, some could pose a future threat to human health, necessitating further studies, ongoing monitoring, and preventive measures.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011552/pdfft?md5=db2021e7efc123faae7304b497a7c610&pid=1-s2.0-S0147651324011552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117065
Exposure to polycyclic aromatic hydrocarbons (PAHs), ubiquitously environmental contaminant, leads to the development of major toxic effects on human health, such as carcinogenic and immunosuppressive alterations reported for the most studied PAH, i.e., benzo(a)pyrene (B(a)P). In order to assess the risk associated with this exposure, it is necessary to have predictive biomarkers. Thus, extracellular vesicles (EVs) and their microRNA (miRNA) contents, have recently been proposed as potentially interesting biomarkers in Toxicology. Our study here explores the use of vesicles secreted and found in blood fluids, and their miRNAs, as biomarkers of exposure to B(a)P alone and within a realistic occupational mixture. We isolated EVs from primary human cultured blood mononuclear cells (PBMCs) and rat plasma after PAH exposure and reported an increased EV production by B(a)P, used either alone or in the mixture, in vitro and in vivo. We then investigated the association of this EV release with the blood concentration of the 7,8,9,10-hydroxy (tetrol)-B(a)P reactive metabolite, in rats. By performing RNA-sequencing (RNA-seq) of miRNAs in PBMC-derived EVs, we analyzed miRNA profiles and demonstrated the regulation of the expression of miR-342–3p upon B(a)P exposure. We then validated B(a)P-induced changes of miR-342–3p expression in vivo in rat plasma-derived EVs. Overall, our study highlights the feasibility of using EVs and their miRNA contents, as biomarkers of PAH exposure and discusses their potential in environmental Toxicology.
{"title":"Exploration of microRNAs from blood extracellular vesicles as biomarkers of exposure to polycyclic aromatic hydrocarbons","authors":"","doi":"10.1016/j.ecoenv.2024.117065","DOIUrl":"10.1016/j.ecoenv.2024.117065","url":null,"abstract":"<div><div>Exposure to polycyclic aromatic hydrocarbons (PAHs), ubiquitously environmental contaminant, leads to the development of major toxic effects on human health, such as carcinogenic and immunosuppressive alterations reported for the most studied PAH, <em>i.e.</em>, benzo(<em>a</em>)pyrene (B(<em>a</em>)P). In order to assess the risk associated with this exposure, it is necessary to have predictive biomarkers. Thus, extracellular vesicles (EVs) and their microRNA (miRNA) contents, have recently been proposed as potentially interesting biomarkers in Toxicology. Our study here explores the use of vesicles secreted and found in blood fluids, and their miRNAs, as biomarkers of exposure to B(<em>a</em>)P alone and within a realistic occupational mixture. We isolated EVs from primary human cultured blood mononuclear cells (PBMCs) and rat plasma after PAH exposure and reported an increased EV production by B(<em>a</em>)P, used either alone or in the mixture, <em>in vitro</em> and <em>in vivo</em>. We then investigated the association of this EV release with the blood concentration of the 7,8,9,10-hydroxy (tetrol)-B(<em>a</em>)P reactive metabolite, in rats. By performing RNA-sequencing (RNA-seq) of miRNAs in PBMC-derived EVs, we analyzed miRNA profiles and demonstrated the regulation of the expression of miR-342–3p upon B(<em>a</em>)P exposure. We then validated B(<em>a</em>)P-induced changes of miR-342–3p expression <em>in vivo</em> in rat plasma-derived EVs. Overall, our study highlights the feasibility of using EVs and their miRNA contents, as biomarkers of PAH exposure and discusses their potential in environmental Toxicology.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011412/pdfft?md5=05ee755899705afe8a8aeab900ef1be4&pid=1-s2.0-S0147651324011412-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117045
The increasing concern over the environmental presence of β-N-Methylamino-L-alanine (BMAA), a toxin primarily produced by cyanobacteria and diatoms, has stimulated numerous studies to evaluate the risk for exposed populations, mainly aquatic organisms and humans. This study focuses on the toxicity of environmental concentrations of BMAA and its isomers, l-2,4 diaminobutyric acid dihydrochloride (DAB) and N-(2-aminoethyl) glycine (AEG) on zebrafish embryo development (ng.L−1). Presence of BMAA in various environments, including aquatic sources, air, and desert crusts, has raised concerns due to its potential link to neurodegenerative diseases such as the amyotrophic lateral sclerosis/parkinsonism dementia complex (ALS/PDC). Despite its known toxicity at high concentrations, there is limited information on the effects of environmental concentrations of BMAA and its isomers. These isomers are often found in association with BMAA and have been detected in seafood intended for human consumption, indicating potential risks from bioaccumulation and biomagnification. Zebrafish embryos have been chosen as a model due to their relevance for embryonic development and toxicity studies. The study employed fish embryo acute toxicity tests and behavioural analyses to specifically assess the sublethal effects of BMAA, DAB, and AEG. The results demonstrated larval mortality rates between 0 % and 3.75 %, while morphological defects were detected across all tested concentrations for each molecule. Behavioural analyses showed alterations in swimming behaviour. Unexpectedly, the changes in morphology and locomotion of the zebrafish larvae were detected more frequently at the lowest concentrations tested, suggesting potential non-monotonic dose responses. Overall, this research underscores the environmental risks associated with BMAA and its isomers, highlighting the importance of continuous monitoring and understanding of their sublethal effects on aquatic organisms and potential implications for human health. Further studies are warranted to elucidate the mechanisms of toxicity, evaluate long-term effects, and assess the risks associated with chronic exposure to these toxins.
{"title":"Effects of environmental concentrations of toxins BMAA and its isomers DAB and AEG on zebrafish larvae","authors":"","doi":"10.1016/j.ecoenv.2024.117045","DOIUrl":"10.1016/j.ecoenv.2024.117045","url":null,"abstract":"<div><div>The increasing concern over the environmental presence of β-N-Methylamino-L-alanine (BMAA), a toxin primarily produced by cyanobacteria and diatoms, has stimulated numerous studies to evaluate the risk for exposed populations, mainly aquatic organisms and humans. This study focuses on the toxicity of environmental concentrations of BMAA and its isomers, l-2,4 diaminobutyric acid dihydrochloride (DAB) and N-(2-aminoethyl) glycine (AEG) on zebrafish embryo development (ng.L<sup>−1</sup>). Presence of BMAA in various environments, including aquatic sources, air, and desert crusts, has raised concerns due to its potential link to neurodegenerative diseases such as the amyotrophic lateral sclerosis/parkinsonism dementia complex (ALS/PDC). Despite its known toxicity at high concentrations, there is limited information on the effects of environmental concentrations of BMAA and its isomers. These isomers are often found in association with BMAA and have been detected in seafood intended for human consumption, indicating potential risks from bioaccumulation and biomagnification. Zebrafish embryos have been chosen as a model due to their relevance for embryonic development and toxicity studies. The study employed fish embryo acute toxicity tests and behavioural analyses to specifically assess the sublethal effects of BMAA, DAB, and AEG. The results demonstrated larval mortality rates between 0 % and 3.75 %, while morphological defects were detected across all tested concentrations for each molecule. Behavioural analyses showed alterations in swimming behaviour. Unexpectedly, the changes in morphology and locomotion of the zebrafish larvae were detected more frequently at the lowest concentrations tested, suggesting potential non-monotonic dose responses. Overall, this research underscores the environmental risks associated with BMAA and its isomers, highlighting the importance of continuous monitoring and understanding of their sublethal effects on aquatic organisms and potential implications for human health. Further studies are warranted to elucidate the mechanisms of toxicity, evaluate long-term effects, and assess the risks associated with chronic exposure to these toxins.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011217/pdfft?md5=3871ccda74db02dd23c57e735472dcd7&pid=1-s2.0-S0147651324011217-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117075
Off-target pesticide drift in paddy fields following unmanned aerial vehicle (UAV) spraying was evaluated using cellulose deposition samplers (CDSs). An analytical method for quantifying ferimzone Z and E isomers deposited on CDSs was developed using LC-MS/MS. The suitability of the CDS method was confirmed by comparing deposition patterns on CDSs with residue levels in rice plant samples. To assess pesticide deposition in paddy fields, CDSs were strategically placed at varying distances from target areas, followed by UAV spraying. The fungicide agrochemicals were applied with and without adjuvants, and wind direction affected the drift trajectory for all treatment groups. Adjuvants, particularly soy lecithin as the major component, significantly enhanced pesticide deposition within the spray pathway while reducing drift rates relatively by 47.9–68.0 %. Higher wind speeds were found to exacerbate drift, but adjuvant-treated sprays showed less variability in deposition patterns under these conditions. Pesticide residues in harvested brown rice were found to be below the maximum residue limits (MRLs), ensuring safety for consumption. These findings highlight the importance of selecting appropriate adjuvants in UAV-based pesticide applications to optimize deposition efficiency and minimize environmental contamination.
使用纤维素沉积采样器（CDS）对无人飞行器（UAV）喷洒后水稻田中的脱靶农药漂移进行了评估。利用 LC-MS/MS 开发了一种分析方法，用于定量分析沉积在 CDS 上的阿维菌素 Z 和 E 异构体。通过比较 CDS 上的沉积模式和水稻植株样本中的残留水平，确认了 CDS 方法的适用性。为了评估水稻田中的农药沉积情况，CDS 被战略性地放置在离目标区域不同的距离，然后进行无人机喷洒。喷洒杀真菌剂农用化学品时使用了或未使用佐剂，风向对所有处理组的漂移轨迹都有影响。佐剂，尤其是作为主要成分的大豆卵磷脂，显著提高了农药在喷洒路径内的沉积，同时相对降低了 47.9-68.0 % 的漂移率。研究发现，较高的风速会加剧漂移，但经过佐剂处理的喷雾在这些条件下的沉积模式变化较小。收获的糙米中农药残留量低于最高残留限量（MRL），确保了食用安全。这些发现凸显了在无人机农药应用中选择适当佐剂的重要性，以优化沉积效率并最大限度地减少环境污染。
{"title":"Comparison of off-target pesticide drift in paddy fields from unmanned aerial vehicle spraying using cellulose deposition sampler","authors":"","doi":"10.1016/j.ecoenv.2024.117075","DOIUrl":"10.1016/j.ecoenv.2024.117075","url":null,"abstract":"<div><div>Off-target pesticide drift in paddy fields following unmanned aerial vehicle (UAV) spraying was evaluated using cellulose deposition samplers (CDSs). An analytical method for quantifying ferimzone Z and E isomers deposited on CDSs was developed using LC-MS/MS. The suitability of the CDS method was confirmed by comparing deposition patterns on CDSs with residue levels in rice plant samples. To assess pesticide deposition in paddy fields, CDSs were strategically placed at varying distances from target areas, followed by UAV spraying. The fungicide agrochemicals were applied with and without adjuvants, and wind direction affected the drift trajectory for all treatment groups. Adjuvants, particularly soy lecithin as the major component, significantly enhanced pesticide deposition within the spray pathway while reducing drift rates relatively by 47.9–68.0 %. Higher wind speeds were found to exacerbate drift, but adjuvant-treated sprays showed less variability in deposition patterns under these conditions. Pesticide residues in harvested brown rice were found to be below the maximum residue limits (MRLs), ensuring safety for consumption. These findings highlight the importance of selecting appropriate adjuvants in UAV-based pesticide applications to optimize deposition efficiency and minimize environmental contamination.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011515/pdfft?md5=f8b13cd38b514d41bff7e418ac4a65fd&pid=1-s2.0-S0147651324011515-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117017
Anthropogenic chemical pollutants, such as fungicides, pose significant threats to natural ecosystems. Although the direct impacts of numerous chemicals are well-documented in simple environmental contexts, their indirect impacts are poorly understood. This study used two individual level laboratory experiments to assess direct and indirect effects of fungicides on the isopod Asellus aquaticus, a keystone detritivore in freshwater systems. First, a range-finding assay on three widely used fungicides (Fluazinam, Tebuconazole, Urea) showed that Tebuconazole had the strongest concentration-dependent negative effects on A. aquaticus growth and food consumption. Second, a factorial experiment using Tebuconazole assessed its direct and diet-mediated effects and showed that Tebuconazole reduced growth, feeding, and pigmentation through both pathways. The results indicate that assessing only direct impacts of toxic chemicals could overlook critical interactions that are relevant in natural systems, such as those associated with diet. Our study highlights the importance of considering both direct and indirect effects in environmental toxicology to better understand the full impacts of chemical pollutants in nature.
{"title":"Direct and indirect effects of chemical pollution: Fungicides alter growth, feeding, and pigmentation of the freshwater detritivore Asellus aquaticus","authors":"","doi":"10.1016/j.ecoenv.2024.117017","DOIUrl":"10.1016/j.ecoenv.2024.117017","url":null,"abstract":"<div><div>Anthropogenic chemical pollutants, such as fungicides, pose significant threats to natural ecosystems. Although the direct impacts of numerous chemicals are well-documented in simple environmental contexts, their indirect impacts are poorly understood. This study used two individual level laboratory experiments to assess direct and indirect effects of fungicides on the isopod <em>Asellus aquaticus</em>, a keystone detritivore in freshwater systems. First, a range-finding assay on three widely used fungicides (Fluazinam, Tebuconazole, Urea) showed that Tebuconazole had the strongest concentration-dependent negative effects on <em>A. aquaticus</em> growth and food consumption. Second, a factorial experiment using Tebuconazole assessed its direct and diet-mediated effects and showed that Tebuconazole reduced growth, feeding, and pigmentation through both pathways. The results indicate that assessing only direct impacts of toxic chemicals could overlook critical interactions that are relevant in natural systems, such as those associated with diet. Our study highlights the importance of considering both direct and indirect effects in environmental toxicology to better understand the full impacts of chemical pollutants in nature.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324010935/pdfft?md5=be22d6d3cbd94055ec6fad5b68f5d6e3&pid=1-s2.0-S0147651324010935-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117078
Copper (Cu) is a common heavy metal and a hazardous environmental pollutant. Emerging epidemiological evidence suggests that Cu exposure is associated with female infertility, especially ovarian dysfunction. However, the mechanisms underlying ovarian toxicity remain poorly understood. Granulosa cells play crucial roles in follicle development and are the main target cells of environmental pollutants for ovarian toxicity. In this study, we investigated the effects of Cu exposure on human granulosa (KGN) cells by using cell biology and metabolomics methods, and explored the molecular mechanisms of Cu-induced cytotoxicity. We found that Cu reduced cell viability in a dose- and time-dependent manner. Then, metabolomic analyses led to the identification of 279, 368 and 466 differentially expressed metabolites (DEMs) in KGN cells exposed to 10, 60 and 240 μM Cu, respectively. Pathway enrichment analysis revealed that high Cu led to disturbances of glutathione metabolism, nucleotide metabolism, glycerophospholipid and ether lipid metabolism. Using cell biological assays, we found that exposure to high Cu significantly decreased the GSH/GSSG ratio and altered the activities of the antioxidant enzymes SOD and CAT. Exposure to high Cu significantly increased the level of mitochondrial ROS. These findings further supported the results revealed by metabolomic analysis and provided clues for elucidating the mechanism by which Cu interferes with the development of ovarian follicles.
{"title":"Metabolic changes induced by heavy metal copper exposure in human ovarian granulosa cells","authors":"","doi":"10.1016/j.ecoenv.2024.117078","DOIUrl":"10.1016/j.ecoenv.2024.117078","url":null,"abstract":"<div><div>Copper (Cu) is a common heavy metal and a hazardous environmental pollutant. Emerging epidemiological evidence suggests that Cu exposure is associated with female infertility, especially ovarian dysfunction. However, the mechanisms underlying ovarian toxicity remain poorly understood. Granulosa cells play crucial roles in follicle development and are the main target cells of environmental pollutants for ovarian toxicity. In this study, we investigated the effects of Cu exposure on human granulosa (KGN) cells by using cell biology and metabolomics methods, and explored the molecular mechanisms of Cu-induced cytotoxicity. We found that Cu reduced cell viability in a dose- and time-dependent manner. Then, metabolomic analyses led to the identification of 279, 368 and 466 differentially expressed metabolites (DEMs) in KGN cells exposed to 10, 60 and 240 μM Cu, respectively. Pathway enrichment analysis revealed that high Cu led to disturbances of glutathione metabolism, nucleotide metabolism, glycerophospholipid and ether lipid metabolism. Using cell biological assays, we found that exposure to high Cu significantly decreased the GSH/GSSG ratio and altered the activities of the antioxidant enzymes SOD and CAT. Exposure to high Cu significantly increased the level of mitochondrial ROS. These findings further supported the results revealed by metabolomic analysis and provided clues for elucidating the mechanism by which Cu interferes with the development of ovarian follicles.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011540/pdfft?md5=53eb3e0cffda6780a7c13da50e2cfc1a&pid=1-s2.0-S0147651324011540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.ecoenv.2024.117066
Perfluorobutane sulfonate (PFBS) is recognized as a highly persistent environmental contaminant, notorious for its chemical stability and enduring presence in ecosystems. Its propensity for persistence and environmental mobility allows PFBS to infiltrate the human body, predominantly accumulating in the liver where it poses a potential risk for hepatic damage. This investigation aimed to explore the outcomes of PFBS on the physiological functionalities of hepatocytes in vitro. To this end, hepatocytes were exposed to 750 ug/ml PFBS, followed by an analysis of various cellular phenotypes and functionalities, including assessments of cell viability and mitochondrial integrity. The findings indicated that PFBS exposure led to a suppression of cell proliferation and an increase in apoptotic cell death. Moreover, PFBS exposure was found to augment the generation of reactive oxygen species (ROS) and induce significant mitochondrial dysfunction. Gene expression analysis identified significant changes in genes associated with numerous tumor signaling pathways and autophagy signaling pathways. Further examinations revealed an increase in cellular mitophagy following PFBS exposure, coupled with the activation of the mitophagy-associated Drp1/Pink1/Parkin pathway. Inhibition of mitophagy was observed to concurrently amplify cellular damage and inhibit the Drp1/Pink1/Parkin pathway. Together, these findings highlight PFBS’s capacity to inflict hepatocyte injury through mitochondrial disruption, positioning Drp1/Pink1/Parkin-mediated mitophagy as a crucial cellular defense mechanism against PFBS-induced toxicity.
{"title":"The role of Drp1 – Pink1 – Parkin – mediated mitophagy in perfluorobutane sulfonate– induced hepatocyte damage","authors":"","doi":"10.1016/j.ecoenv.2024.117066","DOIUrl":"10.1016/j.ecoenv.2024.117066","url":null,"abstract":"<div><div>Perfluorobutane sulfonate (PFBS) is recognized as a highly persistent environmental contaminant, notorious for its chemical stability and enduring presence in ecosystems. Its propensity for persistence and environmental mobility allows PFBS to infiltrate the human body, predominantly accumulating in the liver where it poses a potential risk for hepatic damage. This investigation aimed to explore the outcomes of PFBS on the physiological functionalities of hepatocytes in vitro. To this end, hepatocytes were exposed to 750 ug/ml PFBS, followed by an analysis of various cellular phenotypes and functionalities, including assessments of cell viability and mitochondrial integrity. The findings indicated that PFBS exposure led to a suppression of cell proliferation and an increase in apoptotic cell death. Moreover, PFBS exposure was found to augment the generation of reactive oxygen species (ROS) and induce significant mitochondrial dysfunction. Gene expression analysis identified significant changes in genes associated with numerous tumor signaling pathways and autophagy signaling pathways. Further examinations revealed an increase in cellular mitophagy following PFBS exposure, coupled with the activation of the mitophagy-associated Drp1/Pink1/Parkin pathway. Inhibition of mitophagy was observed to concurrently amplify cellular damage and inhibit the Drp1/Pink1/Parkin pathway. Together, these findings highlight PFBS’s capacity to inflict hepatocyte injury through mitochondrial disruption, positioning Drp1/Pink1/Parkin-mediated mitophagy as a crucial cellular defense mechanism against PFBS-induced toxicity.</div></div>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147651324011424/pdfft?md5=bde4011aeed0ff78665e2e70d7c0c68e&pid=1-s2.0-S0147651324011424-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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