Background & objectives: Endurance activity affects muscles through changes in hormone-secretion and related receptors. The aim of this study was to evaluate the effect of endurance training on Thrap1 gene expression in cardiac tissue and fast and slow twitch skeletal muscles in male Wistar rats. Methods: The subjects of this experimental study were 14 male rats with a mean and standard deviation of 234 ± 34g, all of which were kept in natural conditions (free access to water and food, cycle of darkness and light, suitable temperature and humidity). They were randomly divided into two groups of control (n=7) and experimental (n=7). The experimental group had endurance activities 6 sessions per week at the speed of 30 meters per minute for 14 weeks. 48 hours after the last training session, they were anesthetized and dissected under sterile conditions, and Real- time RT-PCR method was employed to determine the gene expression. Finally,a t-test was used to evaluate the data. Results: The results of this study showed that the expression of the Thrap1 gene in the soleus muscle ( p =0.001) and heart ( p =0.001) of experimental rats increased significantly, while there was not a significant change in the expression of the Thrap1 gene in fast twitch muscles ( p =0.508) due to endurance activity. Conclusions: It seems the expression of the Thrap1 gene in slow twitch muscles is more affected than fast twitch muscles by endurance activity.
{"title":"The effect of Endurance Training on Expression of T3 Receptor-Associated Protein-1 (THRAP-1) in Heart, Slow and Fast Twitch Muscles","authors":"M. Fathi, R. Rezaei, M. Bahrami","doi":"10.52547/jarums.22.1.84","DOIUrl":"https://doi.org/10.52547/jarums.22.1.84","url":null,"abstract":"Background & objectives: Endurance activity affects muscles through changes in hormone-secretion and related receptors. The aim of this study was to evaluate the effect of endurance training on Thrap1 gene expression in cardiac tissue and fast and slow twitch skeletal muscles in male Wistar rats. Methods: The subjects of this experimental study were 14 male rats with a mean and standard deviation of 234 ± 34g, all of which were kept in natural conditions (free access to water and food, cycle of darkness and light, suitable temperature and humidity). They were randomly divided into two groups of control (n=7) and experimental (n=7). The experimental group had endurance activities 6 sessions per week at the speed of 30 meters per minute for 14 weeks. 48 hours after the last training session, they were anesthetized and dissected under sterile conditions, and Real- time RT-PCR method was employed to determine the gene expression. Finally,a t-test was used to evaluate the data. Results: The results of this study showed that the expression of the Thrap1 gene in the soleus muscle ( p =0.001) and heart ( p =0.001) of experimental rats increased significantly, while there was not a significant change in the expression of the Thrap1 gene in fast twitch muscles ( p =0.508) due to endurance activity. Conclusions: It seems the expression of the Thrap1 gene in slow twitch muscles is more affected than fast twitch muscles by endurance activity.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48241538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background & objectives: Tissue engineering is a growing field to repair and replace the defective function of damaged tissue or organ, and today it is proposed as a new treatment to replace conventional transplant methods. For this purpose, polymeric biomaterials (scaffolds) and living cells are used. The purpose of this study is to fabricate polycaprolactan (PCL) nanoscaffold and load silymarin on the nanoscaffold to check the biocompatibility and proliferation ability of pc12 cells on it. Methods: In order to prepare polycaprolactan nanoscaffold and load silymarin on it, 7% polycaprolactan solution (dissolved in acetic acid) was mixed with silymarin solution with a concentration of 0.9% (weight percent), and then the scaffold was prepared using electrospinning device. The morphology of the scaffold was evaluated by scanning electron microscope (SEM) and the chemical structure of the scaffold was evaluated by ATR-FTIR spectroscopy. Toxicity of the scaffold and cell survival of PC12 cells were investigated by MTT test and SEM microscope respectively. Results: Examining the morphology of the scaffold and its chemical structure showed the appropriate porosity of the scaffold and the successful loading of silymarin on the PCL scaffold. The toxicity of the scaffold was investigated 24, 48 and 72 hours after the cultivation of PC12 cells, and the results showed an increase in cell viability and proper attachment of cells on the scaffold. Conclusion: The results of this research showed that the loading of silymarin on polycaprolactan scaffold increases the proliferation and survival of PC12 cells. Therefore, this scaffold can be a suitable candidate for nerve tissue engineering.
{"title":"Fabrication of Nanofiber Scaffolds Using Polycaprolactan Containing Silymarin to Study Neural Cell Tissue Engineering","authors":"R. Najafi, A. Asadi, S. Zahri, A. Abdolmaleki","doi":"10.52547/jarums.22.1.7","DOIUrl":"https://doi.org/10.52547/jarums.22.1.7","url":null,"abstract":"Background & objectives: Tissue engineering is a growing field to repair and replace the defective function of damaged tissue or organ, and today it is proposed as a new treatment to replace conventional transplant methods. For this purpose, polymeric biomaterials (scaffolds) and living cells are used. The purpose of this study is to fabricate polycaprolactan (PCL) nanoscaffold and load silymarin on the nanoscaffold to check the biocompatibility and proliferation ability of pc12 cells on it. Methods: In order to prepare polycaprolactan nanoscaffold and load silymarin on it, 7% polycaprolactan solution (dissolved in acetic acid) was mixed with silymarin solution with a concentration of 0.9% (weight percent), and then the scaffold was prepared using electrospinning device. The morphology of the scaffold was evaluated by scanning electron microscope (SEM) and the chemical structure of the scaffold was evaluated by ATR-FTIR spectroscopy. Toxicity of the scaffold and cell survival of PC12 cells were investigated by MTT test and SEM microscope respectively. Results: Examining the morphology of the scaffold and its chemical structure showed the appropriate porosity of the scaffold and the successful loading of silymarin on the PCL scaffold. The toxicity of the scaffold was investigated 24, 48 and 72 hours after the cultivation of PC12 cells, and the results showed an increase in cell viability and proper attachment of cells on the scaffold. Conclusion: The results of this research showed that the loading of silymarin on polycaprolactan scaffold increases the proliferation and survival of PC12 cells. Therefore, this scaffold can be a suitable candidate for nerve tissue engineering.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45738121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.441
Soheila Derisavy Jorfian, E. Hajmohammadi, Erfan Belalzadeh
Intraosseous mucoepidermoid carcinoma is a rare lesion that is mostly seen in the mandible and in the premolar and molar region. In some cases, clear cells can be seen, which makes it difficult to diagnose. The purpose of this case report is the clinical and histopathological evaluation of the clear cell variant of hntraosseous mucoepidermoid carcinoma in the anterior region of the lower jaw that was referred to the dentistry faculty of Ardabil University of Medical Sciences.
{"title":"Clear Cell Variant of Intraosseous Mucoepidermoid Carcinoma in the Anterior Region of the Mandible; a Case Report","authors":"Soheila Derisavy Jorfian, E. Hajmohammadi, Erfan Belalzadeh","doi":"10.52547/jarums.21.4.441","DOIUrl":"https://doi.org/10.52547/jarums.21.4.441","url":null,"abstract":"Intraosseous mucoepidermoid carcinoma is a rare lesion that is mostly seen in the mandible and in the premolar and molar region. In some cases, clear cells can be seen, which makes it difficult to diagnose. The purpose of this case report is the clinical and histopathological evaluation of the clear cell variant of hntraosseous mucoepidermoid carcinoma in the anterior region of the lower jaw that was referred to the dentistry faculty of Ardabil University of Medical Sciences.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48320576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.416
Mehdi Hoseinnezhad, N. Hamidi, J. Mehrabi
Background & objectives: This research has been carried out in order to optimize technology development policies and reduce the costs of health technology development centers in universities of medical sciences. Identifying the influential factors in the growth of technology units helps the growth centers to more efficiently use the growth of knowledge-based companies and the development and progress of innovative entrepreneurs as a host environment for technology growth. Methods : The current study is a qualitative research that was conducted by extracting qualitative data through semi-structured interviews with 31 managers of growth centers and managers of units located in growth centers and experts in the field of health entrepreneurship. It has been coded and categorized in three stages and the main and subcategories have been identified. Results: The results show that for the growth of technology units in health technology growth centers, two basic variables of the growth center program and infrastructure have an effect on the efficiency of the centers and the growth of the units. This is despite the fact that according to the regional capacities of Type 3 medical sciences universities in the country, growth program factors have introduced more categories and less focus has been placed on infrastructure, so the growth plan is good. It can have a bigger share. Conclusion: This research shows what categories are expected from the growth plan and infrastructures of health technology growth centers in few universities.
{"title":"Identifying the Factors Affecting the Growth in Health Technology Incubator of Medical Universities of the Country (Study Three Type Medical Universities)","authors":"Mehdi Hoseinnezhad, N. Hamidi, J. Mehrabi","doi":"10.52547/jarums.21.4.416","DOIUrl":"https://doi.org/10.52547/jarums.21.4.416","url":null,"abstract":"Background & objectives: This research has been carried out in order to optimize technology development policies and reduce the costs of health technology development centers in universities of medical sciences. Identifying the influential factors in the growth of technology units helps the growth centers to more efficiently use the growth of knowledge-based companies and the development and progress of innovative entrepreneurs as a host environment for technology growth. Methods : The current study is a qualitative research that was conducted by extracting qualitative data through semi-structured interviews with 31 managers of growth centers and managers of units located in growth centers and experts in the field of health entrepreneurship. It has been coded and categorized in three stages and the main and subcategories have been identified. Results: The results show that for the growth of technology units in health technology growth centers, two basic variables of the growth center program and infrastructure have an effect on the efficiency of the centers and the growth of the units. This is despite the fact that according to the regional capacities of Type 3 medical sciences universities in the country, growth program factors have introduced more categories and less focus has been placed on infrastructure, so the growth plan is good. It can have a bigger share. Conclusion: This research shows what categories are expected from the growth plan and infrastructures of health technology growth centers in few universities.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47257243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.402
Mahsa Mahboob, S. Basharpour, Nader Hajilou, Elham Anbari, H. Salvat
Background & objectives: The purpose of this study was to model the causal behaviors related to bullying based on the experience of child abuse mediating the role of emotional security. Methods: Method of this research was correlational. The statistical population of this study included male high school students in Ardabil city in the academic year of 97-98 in which 385 people were selected as a statistical sample by cluster random sampling. Bernstein's Child abuse (CTQ) and Emotional Security Questionnaires were used to collect data. Data were analyzed using structural equation modeling using AMOS22 and SPSS22 software. Results: The results of the study were in line with the hypotheses that showed child abuse ( p <0.05) had a positive effect and emotional security ( p <0.05) had a negative effect on bullying-related behaviors. Also, the fit of the proposed model has a good fit and the variable power of emotional security in mediating the impact of child abuse on bullying-related behaviors was confirmed. Conclusion: According to the results, parents should be aware of the devastating consequences of child abuse on their children's mental and physical health and the importance of emotional security and safety in their children's healthy development, to prevent and reduce bullying-related behaviors. Teachers and school officials should also be aware of bullying and related behaviors to reduce and prevent harmful effects on students.
{"title":"Causal Modeling of Bullying-related Behaviors based on the Experience of Child Abuse the Mediating Role of Emotional Safety","authors":"Mahsa Mahboob, S. Basharpour, Nader Hajilou, Elham Anbari, H. Salvat","doi":"10.52547/jarums.21.4.402","DOIUrl":"https://doi.org/10.52547/jarums.21.4.402","url":null,"abstract":"Background & objectives: The purpose of this study was to model the causal behaviors related to bullying based on the experience of child abuse mediating the role of emotional security. Methods: Method of this research was correlational. The statistical population of this study included male high school students in Ardabil city in the academic year of 97-98 in which 385 people were selected as a statistical sample by cluster random sampling. Bernstein's Child abuse (CTQ) and Emotional Security Questionnaires were used to collect data. Data were analyzed using structural equation modeling using AMOS22 and SPSS22 software. Results: The results of the study were in line with the hypotheses that showed child abuse ( p <0.05) had a positive effect and emotional security ( p <0.05) had a negative effect on bullying-related behaviors. Also, the fit of the proposed model has a good fit and the variable power of emotional security in mediating the impact of child abuse on bullying-related behaviors was confirmed. Conclusion: According to the results, parents should be aware of the devastating consequences of child abuse on their children's mental and physical health and the importance of emotional security and safety in their children's healthy development, to prevent and reduce bullying-related behaviors. Teachers and school officials should also be aware of bullying and related behaviors to reduce and prevent harmful effects on students.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47043579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.361
S. Banaei
Background& objectives: Renal ischemia-reperfusion (IR) damage occurs during renal transplantation in end-stage renal disease (ESRD) patients which activate immune responses. Inflammatory responses by increased levels of cytokines can lead to acute kidney injury (AKI) that contributes to the loss of renal grafts and graft dysfunction. The purpose of this study was to review the therapeutic effects of nanoparticles in AKI. Methods: A comprehensive search strategy was identified relevant studies on AKI models, using the Scopus, PubMed and Google Scholar databases, from 2000 until 2020. The search strategy included keywords like ischemia-reperfusion and nanoparticles. Results: Oxygen free radicals are produced during the reperfusion phase, which cause lipid peroxidation and promote tissue damage. Oxidative damage to DNA and proteins and lipid membrane peroxidation can cause cell death and apoptosis. Some strategies to reduce the tissue damage caused by ischemia-reperfusion are nanoscale materials. Antioxidant nanoparticles reduce oxidative stress in tissues. Also, they have flexibility in the delivery of therapeutic agents and drugs to the ischemic cells, and imaging of the ischemic regions at the molecular or cellular level. Conclusion: This potential of antioxidant and anti-inflammatory nanoparticles in the diagnosis and treatment of renal ischemic regions is an innovation in the development of new therapies and a unique achievement in recent medical advances.
{"title":"The Protective Effect of Antioxidant and Anti-inflammatory Nanoparticles in Renal Ischemia-Reperfusion Damage","authors":"S. Banaei","doi":"10.52547/jarums.21.4.361","DOIUrl":"https://doi.org/10.52547/jarums.21.4.361","url":null,"abstract":"Background& objectives: Renal ischemia-reperfusion (IR) damage occurs during renal transplantation in end-stage renal disease (ESRD) patients which activate immune responses. Inflammatory responses by increased levels of cytokines can lead to acute kidney injury (AKI) that contributes to the loss of renal grafts and graft dysfunction. The purpose of this study was to review the therapeutic effects of nanoparticles in AKI. Methods: A comprehensive search strategy was identified relevant studies on AKI models, using the Scopus, PubMed and Google Scholar databases, from 2000 until 2020. The search strategy included keywords like ischemia-reperfusion and nanoparticles. Results: Oxygen free radicals are produced during the reperfusion phase, which cause lipid peroxidation and promote tissue damage. Oxidative damage to DNA and proteins and lipid membrane peroxidation can cause cell death and apoptosis. Some strategies to reduce the tissue damage caused by ischemia-reperfusion are nanoscale materials. Antioxidant nanoparticles reduce oxidative stress in tissues. Also, they have flexibility in the delivery of therapeutic agents and drugs to the ischemic cells, and imaging of the ischemic regions at the molecular or cellular level. Conclusion: This potential of antioxidant and anti-inflammatory nanoparticles in the diagnosis and treatment of renal ischemic regions is an innovation in the development of new therapies and a unique achievement in recent medical advances.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44673333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.429
Mehrdad Tashakkori Ghanbarian, S. Naghibi, Mohammad Shariatzadeh Jonaidi, Shahrzad Ansari
Background & objective : FoxO1 and STRA13 proteins play an important role in duplication and cellular metabolism, regulation of cell differentiation, apoptosis and reducing the spread of fat tissue in the body. The aim of this study was to investigate the effect of different intensities of aerobic training on the expression level of FoxO1 and STRA13 genes in subcutaneous adipose tissue of male Wistar rats. Methods: In this experimental study, 32 male Wistar rats with a mean age of 8 weeks and weight of 237±33 gr were selected, and they were randomly divided into 4 equal groups including moderate-intensity aerobic training (MIT), high-intensity aerobic training (HIT), high-intensity interval aerobic training (HIIT) and control group. The training program was implemented 8 weeks and 5 sessions per week for the experimental groups,. Adipose tissue biopsy was performed 48 hours after the last training session to evaluate FoxO1 and STRA13 gene expression using RT-PCR method. Data were analyzed using One-way analysis of variance test using SPSS 24 software at the significance level of p <0.05. Results: The results showed that there was a significant difference in the FoxO1 gene expression level in the subcutaneous tissue of male Wistar rats between HIT and control groups ( p =0.0001). However, no significant difference was observed between experimental groups. In addition, there was a significant difference in STRA13 gene expression level in the subcutaneous tissue between MIT ( p =0.008), HIT ( p =0.0001) and HIIT ( p =0.009) groups and control group. Conclusion: According to the results, aerobic exercise with variety of intensity is effective in controlling the genes expression rate involved in fat metabolism and by reducing the FoxO1 and STRA13 genes expression, they cause the duplication and reduce the expansion of fat tissue.
{"title":"The Effects of Aerobic Training with Different Intensity on FoxO1 and STRA13 Genes Expression in Subcutaneous Fat Tissue of Male Wistar Rat","authors":"Mehrdad Tashakkori Ghanbarian, S. Naghibi, Mohammad Shariatzadeh Jonaidi, Shahrzad Ansari","doi":"10.52547/jarums.21.4.429","DOIUrl":"https://doi.org/10.52547/jarums.21.4.429","url":null,"abstract":"Background & objective : FoxO1 and STRA13 proteins play an important role in duplication and cellular metabolism, regulation of cell differentiation, apoptosis and reducing the spread of fat tissue in the body. The aim of this study was to investigate the effect of different intensities of aerobic training on the expression level of FoxO1 and STRA13 genes in subcutaneous adipose tissue of male Wistar rats. Methods: In this experimental study, 32 male Wistar rats with a mean age of 8 weeks and weight of 237±33 gr were selected, and they were randomly divided into 4 equal groups including moderate-intensity aerobic training (MIT), high-intensity aerobic training (HIT), high-intensity interval aerobic training (HIIT) and control group. The training program was implemented 8 weeks and 5 sessions per week for the experimental groups,. Adipose tissue biopsy was performed 48 hours after the last training session to evaluate FoxO1 and STRA13 gene expression using RT-PCR method. Data were analyzed using One-way analysis of variance test using SPSS 24 software at the significance level of p <0.05. Results: The results showed that there was a significant difference in the FoxO1 gene expression level in the subcutaneous tissue of male Wistar rats between HIT and control groups ( p =0.0001). However, no significant difference was observed between experimental groups. In addition, there was a significant difference in STRA13 gene expression level in the subcutaneous tissue between MIT ( p =0.008), HIT ( p =0.0001) and HIIT ( p =0.009) groups and control group. Conclusion: According to the results, aerobic exercise with variety of intensity is effective in controlling the genes expression rate involved in fat metabolism and by reducing the FoxO1 and STRA13 genes expression, they cause the duplication and reduce the expansion of fat tissue.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49025556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.374
Ensieh Ahmadpour, M. Piri, Mohammad Ali Azarbijani
Background & objective: Alzheimer's disease (AD) is the most common cause of dementia among the elderly, threatening their quality of life. On the other hand, regular exercise is associated with improved brain health and cognitive function. However, the mechanisms of these benefits have not yet been fully elucidated. Therefore, the aim of this study was to investigate the effect of 4 weeks of moderate intensity interval aerobic training on cognitive function and expression level of PGC1α and VEGF genes in the hippocampus of old rats with AD. Methods: For this purpose, 20-month-old male Wistar rats were divided into three groups of AD (n=8), AD+exercise training (n=8) and control (n=8). Intra-hippocampal injection of Aβ 42 was used to induce AD. The animals in the exercise group performed moderate-intensity interval aerobic exercise for 4 weeks, 5 days a week. To assess spatial learning and memory, the animals underwent the Morris water maze test 48 hours following the last training session. Then, the animals were killed and hippocampal tissue was extracted. Real time-PCR method was used to measure gene expression. Statistical analysis was performed using one-way analysis of variance and Pearson correlation coefficient at the significance level of p 0.05. Results: The results showed that Aβ 42 injection impaired spatial learning and memory function and reduced the expression level of PGC1α and VEGF genes in hippocampal tissue ( p 0.05). Aerobic exercise improved spatial learning and memory function and increased PGC1α and VEGF genes expression ( p 0.01). Also, a significant positive relationship was observed between the PGC1α and VEGF gene expression levels in the hippocampus (r= 0.859, p ≤0.01). In addition, there was a significant inverse relationship between PGC1α and VEGF genes expression and the mean time spent to find the platform (r= -0.9, p 0.01 and r= -0.750, p 0.01, respectively), and a significant positive relationship with the time spent in the target quadrant (r= -0.794, p 0.01 and r= -0.632, p 0.01, respectively). Conclusion: In general, aerobic training improves spatial learning and memory performance in old animals with AD; up-regulation of the exercise-induced PGC1α/VEGF pathway in the brain, at least in part, appears to be involved in this adaptation.
{"title":"The Effect of 4 Weeks of Aerobic Training on Cognitive Function and Expression Level of PGC1α and VEGF Genes in the Hippocampus of Rats with Alzheimer’s Disease","authors":"Ensieh Ahmadpour, M. Piri, Mohammad Ali Azarbijani","doi":"10.52547/jarums.21.4.374","DOIUrl":"https://doi.org/10.52547/jarums.21.4.374","url":null,"abstract":"Background & objective: Alzheimer's disease (AD) is the most common cause of dementia among the elderly, threatening their quality of life. On the other hand, regular exercise is associated with improved brain health and cognitive function. However, the mechanisms of these benefits have not yet been fully elucidated. Therefore, the aim of this study was to investigate the effect of 4 weeks of moderate intensity interval aerobic training on cognitive function and expression level of PGC1α and VEGF genes in the hippocampus of old rats with AD. Methods: For this purpose, 20-month-old male Wistar rats were divided into three groups of AD (n=8), AD+exercise training (n=8) and control (n=8). Intra-hippocampal injection of Aβ 42 was used to induce AD. The animals in the exercise group performed moderate-intensity interval aerobic exercise for 4 weeks, 5 days a week. To assess spatial learning and memory, the animals underwent the Morris water maze test 48 hours following the last training session. Then, the animals were killed and hippocampal tissue was extracted. Real time-PCR method was used to measure gene expression. Statistical analysis was performed using one-way analysis of variance and Pearson correlation coefficient at the significance level of p 0.05. Results: The results showed that Aβ 42 injection impaired spatial learning and memory function and reduced the expression level of PGC1α and VEGF genes in hippocampal tissue ( p 0.05). Aerobic exercise improved spatial learning and memory function and increased PGC1α and VEGF genes expression ( p 0.01). Also, a significant positive relationship was observed between the PGC1α and VEGF gene expression levels in the hippocampus (r= 0.859, p ≤0.01). In addition, there was a significant inverse relationship between PGC1α and VEGF genes expression and the mean time spent to find the platform (r= -0.9, p 0.01 and r= -0.750, p 0.01, respectively), and a significant positive relationship with the time spent in the target quadrant (r= -0.794, p 0.01 and r= -0.632, p 0.01, respectively). Conclusion: In general, aerobic training improves spatial learning and memory performance in old animals with AD; up-regulation of the exercise-induced PGC1α/VEGF pathway in the brain, at least in part, appears to be involved in this adaptation.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44600065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.447
Shafagh Aliasgarzade, M. Negaresh, V. Aghamohammadi, Nazli Javaheri, Javad Aliasgarzade, sevda Mikaeili Mirak
Neuroarthropathy occurs following the loss of pain sensation in a joint. Martin Charcot was the first researcher who explained this strange, non-infectious destruction of bone and joints. This disease mostly affects the lower extremity of patients, especially their feet and ankle. In this paper, we present a rare case of simultaneous neuroarthropathy in the wrist and ankle of a 39-year-old man with no history of diabetes. Simultaneous neuroarthropathy of two joints and its occurrence in uncommon parts of the body like the wrist is quite rare and might lead to misdiagnosis and incorrect treatment.
{"title":"A Case of Simultaneous Neuroarthropathy in the Wrist and Ankle with No History of Diabetes: Case Report","authors":"Shafagh Aliasgarzade, M. Negaresh, V. Aghamohammadi, Nazli Javaheri, Javad Aliasgarzade, sevda Mikaeili Mirak","doi":"10.52547/jarums.21.4.447","DOIUrl":"https://doi.org/10.52547/jarums.21.4.447","url":null,"abstract":"Neuroarthropathy occurs following the loss of pain sensation in a joint. Martin Charcot was the first researcher who explained this strange, non-infectious destruction of bone and joints. This disease mostly affects the lower extremity of patients, especially their feet and ankle. In this paper, we present a rare case of simultaneous neuroarthropathy in the wrist and ankle of a 39-year-old man with no history of diabetes. Simultaneous neuroarthropathy of two joints and its occurrence in uncommon parts of the body like the wrist is quite rare and might lead to misdiagnosis and incorrect treatment.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47370228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.52547/jarums.21.4.388
Maryam Zolfali Pourfar, Farhad Rahmani Nia, P. Farzanegi
Background & objectives: Dynamic disorders of mitochondria cause the pathogenesis of many diseases, such as type 2 diabetes. Therefore, the aim of this study was to investigate the interactive effect of aerobic exercises and atorvastatin consumption on the expression of MFN1/2 and DRP1 in hepatocytes of rat liver with type 2 diabetes. Methods: In this experimental study, 25 male rats were divided into 5 equal groups: diabetes, healthy control, persistence+diabetes, atorvastatin+diabetes, persistence+atorvastatin+ diabetes. Type 2 diabetes was induced by streptozotocin (STZ) in mice. The training groups performed the running program on the treadmill for eight weeks. Atorvastatin and atorvastatin-exercise groups received atorvastatin (10 mg/kg) by gavage. 48 hours after the last training session, the rats were dissected; their liver tissue was removed and immediately frozen in liquid nitrogen solution at a temperature of minus 80 ° C to measure MFN1/2 and DRP1. One-way analysis of variance and Tukey post hoc test were used for statistical analysis at a significance level of p <0.05. Results: The results showed that induction of type 2 diabetes decreased the expression of MFN1/2 and increased DRP1 compared to the healthy group. After eight weeks of intervention, a significant increase was observed in the expression level of MFN1 ( p <0.05), but this increase was not significant in MFN2 and there was no significant difference in the expression of factors between the groups. Also, after eight weeks, a significant decrease in DRP1 gene expression was observed ( p <0.05). This decrease was significant in comparison with the combined groups compared to the patient group. Conclusion: It is possible that a combination of aerobic exercises and atorvastatin may positively regulate the expression of genes related to mitochondrial dynamics in diabetes.
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