Pub Date : 2016-09-04DOI: 10.21157/J.KED.HEWAN.V10I2.5044
M. Murniati, Etih Sudarnika, Y. Ridwan
The aims of this research were to estimate the prevalence and to determine the risk factors related to Toxocara cati (T. cati) infection in pet cats in Bogor. This study was cross sectional study using two types of data; laboratory examination results of feces samples and interview results of the cat owner using a structured questionnaires including characteristics of pet owner and pet care management. The data obtained from this study was analyzed using chi-square test. The results showed that 85 of 243 cats were positively infected by T. cati with 35% prevalence. The significant risk factor associated with the T. cati infection were: sex, sand provided, deworming and type of feed. Pet care management in Kota Bogor were well managed, however the prevalence of T. cati infection was still high. Therefore it can be a potential public health problem especially on cat owners.
{"title":"PREVALENSI DAN FAKTOR RISIKO INFEKSI Toxocara cati PADA KUCING PELIHARAAN DI KOTA BOGOR (Prevalence and Risk Factors of Toxocara cati Infection in Bogor Pet Cats)","authors":"M. Murniati, Etih Sudarnika, Y. Ridwan","doi":"10.21157/J.KED.HEWAN.V10I2.5044","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I2.5044","url":null,"abstract":"The aims of this research were to estimate the prevalence and to determine the risk factors related to Toxocara cati (T. cati) infection in pet cats in Bogor. This study was cross sectional study using two types of data; laboratory examination results of feces samples and interview results of the cat owner using a structured questionnaires including characteristics of pet owner and pet care management. The data obtained from this study was analyzed using chi-square test. The results showed that 85 of 243 cats were positively infected by T. cati with 35% prevalence. The significant risk factor associated with the T. cati infection were: sex, sand provided, deworming and type of feed. Pet care management in Kota Bogor were well managed, however the prevalence of T. cati infection was still high. Therefore it can be a potential public health problem especially on cat owners.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"139-142"},"PeriodicalIF":0.0,"publicationDate":"2016-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-04DOI: 10.21157/J.KED.HEWAN.V10I2.5091
Nu'man - Hidayat, C. Sumantri, R. Afnan
The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P<0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.
{"title":"PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation)","authors":"Nu'man - Hidayat, C. Sumantri, R. Afnan","doi":"10.21157/J.KED.HEWAN.V10I2.5091","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I2.5091","url":null,"abstract":"The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P<0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"170-174"},"PeriodicalIF":0.0,"publicationDate":"2016-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-03DOI: 10.21157/J.KED.HEWAN.V10I2.5038
M. Bahi, C. Jacob, K. Khairan
The objective of this research was to investigate cytotoxicity effect of Haarlem oil (HO) on HL-60 cell line and Steinernema feltiae (S. feltiae). The test results using trypan blue method and CellTiter-Glo® assays revealed that HO showed higher cytotoxicity effect against HL-60 cell line especially at concentration of 1:10 and 1:5. Meanwhile, the MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay revealed that the higher cytotoxicity effect showed at 1:5, with the cell viabilities were around 40-65%. The nematicidal assay discovered that HO at concentration of 1:5 showed higher activities compare to other concentrations with percentage viability of 70%. These studies have shown that HO shows cytotoxic effect against HL-60 cell lines, and moderate activity against S. feltiae as well.
{"title":"EFEK SITOTOKSIK HAARLEM OIL TERHADAP HL-60 CELL LINE DAN Steinernema feltiae (Cytotoxic Effect of Haarlem Oil on HL-60 Cell Line and Steinernema feltiae)","authors":"M. Bahi, C. Jacob, K. Khairan","doi":"10.21157/J.KED.HEWAN.V10I2.5038","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I2.5038","url":null,"abstract":"The objective of this research was to investigate cytotoxicity effect of Haarlem oil (HO) on HL-60 cell line and Steinernema feltiae (S. feltiae). The test results using trypan blue method and CellTiter-Glo® assays revealed that HO showed higher cytotoxicity effect against HL-60 cell line especially at concentration of 1:10 and 1:5. Meanwhile, the MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay revealed that the higher cytotoxicity effect showed at 1:5, with the cell viabilities were around 40-65%. The nematicidal assay discovered that HO at concentration of 1:5 showed higher activities compare to other concentrations with percentage viability of 70%. These studies have shown that HO shows cytotoxic effect against HL-60 cell lines, and moderate activity against S. feltiae as well.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"110-114"},"PeriodicalIF":0.0,"publicationDate":"2016-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3368
K. Khairan, M. Bahi, C. Jacob, R. Idroes
This study was purposed to inquire the activities of benzopyran (chromones) and benzofuranones (coumaranones) derivatives against Steinernema feltiae (S. feltiae). The toxicity assay against S. feltiae showed that benzopyran derivatives 2, 3, 4, 6, and 9 have the highest activity on S. feltiae with viabilities percentage of <50%. The compound 9 demostrated the highest activity with LD 50 and LD values, 7.2 and 52.2 μM, respectively. The activities of compound 7 and 10 showed the lowest toxicity. Interestingly, the activity of benzofuranone derivatives showed significant activities against S. feltiae. Compare to benzopyran derivatives, the benzofuranone derivatives has the highest toxicity, in particular compound 13 with LD 5.45 μM. The nematicidal assay showed that benzofuranones (coumaranones) derivatives revealed higher activities than benzopyran (chromones) derivatives. Key words: chromones, benzopyran, coumaranones, benzofuranone, and Steinernema feltiae
本研究旨在探讨苯并吡喃(色酮)和苯并呋喃酮(香豆酮)衍生物对feltiae (S. feltiae) steinnema feltiae的活性。结果表明，苯并吡喃衍生物2、3、4、6、9对菲氏夜蛾的毒力最强，毒力<50%。其中化合物9的活性最高，LD值分别为7.2 μM和52.2 μM。化合物7和10的毒性最低。有趣的是，苯并呋喃酮衍生物的活性显示出显著的对飞蛾的活性。与苯并吡喃衍生物相比，苯并呋喃酮衍生物的毒性最高，特别是化合物13，其LD为5.45 μM。杀线虫实验表明，苯并呋喃酮(香豆酮)衍生物的杀线虫活性高于苯并吡喃(色酮)衍生物。关键词:色酮，苯并吡喃，香豆酮，苯并呋喃酮，斯坦纳菌
{"title":"The Activities of Benzopyran (Chromones) and Benzofuranones (Coumaranones) Derivatives against Steinernema feltiae","authors":"K. Khairan, M. Bahi, C. Jacob, R. Idroes","doi":"10.21157/J.KED.HEWAN.V10I1.3368","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3368","url":null,"abstract":"This study was purposed to inquire the activities of benzopyran (chromones) and benzofuranones (coumaranones) derivatives against Steinernema feltiae (S. feltiae). The toxicity assay against S. feltiae showed that benzopyran derivatives 2, 3, 4, 6, and 9 have the highest activity on S. feltiae with viabilities percentage of <50%. The compound 9 demostrated the highest activity with LD 50 and LD values, 7.2 and 52.2 μM, respectively. The activities of compound 7 and 10 showed the lowest toxicity. Interestingly, the activity of benzofuranone derivatives showed significant activities against S. feltiae. Compare to benzopyran derivatives, the benzofuranone derivatives has the highest toxicity, in particular compound 13 with LD 5.45 μM. The nematicidal assay showed that benzofuranones (coumaranones) derivatives revealed higher activities than benzopyran (chromones) derivatives. Key words: chromones, benzopyran, coumaranones, benzofuranone, and Steinernema feltiae","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"37-42"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3379
I. Hernaman, Kundrat Hidajat, Atun Budiman, S. Nurachma
The study aimed to evaluate the utilization of cassava plantation waste suplemented with zinc and cobalt on ewe reproduction performance. Six pregnacy ewes were allocated at experimentrations, i.e. K1: control (50% fresh grass + 50% concentrate) and K2: 50% fresh grass + 50% cassava plantation waste + 30 ppm zinc + 5 ppm cobalt. The results showed that the concentration of zinc and cobalt in blood of ewe in group K1 and K1 were 7.03 vs 8.22 ppm and 0.48 vs 0.44 ppm, respectively, while in faeces were 147,72 vs 158.59 ppm and 8.3 vs 9.8 ppm. In addition, the concentration of alkaline phosphatase and progresterone in blood were 116 vs 196 u/l and 0.56 vs 0.59 mg/ml, respectively. The average of offsprings weight on 24 day postpartum in K1 and K2 was 3.48 vs 3.16kg. In conclusion, cassava plantation waste could be used as feed for pregnancy and lactating ewe. Supplementation of 30 ppm zinc is efectively increase the concentration of zinc and alkaline phosphatase in blood . Key words: cobalt, reproduction, cassava, zinc, ewe
本试验旨在评价木薯人工林废弃物补锌补钴对母羊繁殖性能的影响。6只怀孕母羊被分配在试验中，即K1:对照(50%鲜草+ 50%精料)和K2: 50%鲜草+ 50%木薯种植园废弃物+ 30 ppm锌+ 5 ppm钴。结果表明，K1组和K1组母羊血液中锌和钴的浓度分别为7.03 vs 8.22 ppm和0.48 vs 0.44 ppm，而粪便中锌和钴的浓度分别为147、72 vs 158.59 ppm和8.3 vs 9.8 ppm。血中碱性磷酸酶和孕酮浓度分别为116和196 u/l和0.56和0.59 mg/ml。K1和K2组产后24 d子代平均体重分别为3.48和3.16kg。综上所述，木薯种植废弃物可作为妊娠和哺乳期母羊的饲料。补充30ppm锌可有效提高血中锌和碱性磷酸酶的浓度。关键词:钴，繁殖，木薯，锌，母羊
{"title":"PEMBERIAN LIMBAH PERKEBUNAN SINGKONG YANG DISUPLEMENTASI SENG DAN KOBALT TERHADAP PERFORMA REPRODUKSI DOMBA BETINA (Utilization of Cassava Plantation Waste Supplemented with Zinc and Cobalton Reproduction Performance of Ewe)","authors":"I. Hernaman, Kundrat Hidajat, Atun Budiman, S. Nurachma","doi":"10.21157/J.KED.HEWAN.V10I1.3379","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3379","url":null,"abstract":"The study aimed to evaluate the utilization of cassava plantation waste suplemented with zinc and cobalt on ewe reproduction performance. Six pregnacy ewes were allocated at experimentrations, i.e. K1: control (50% fresh grass + 50% concentrate) and K2: 50% fresh grass + 50% cassava plantation waste + 30 ppm zinc + 5 ppm cobalt. The results showed that the concentration of zinc and cobalt in blood of ewe in group K1 and K1 were 7.03 vs 8.22 ppm and 0.48 vs 0.44 ppm, respectively, while in faeces were 147,72 vs 158.59 ppm and 8.3 vs 9.8 ppm. In addition, the concentration of alkaline phosphatase and progresterone in blood were 116 vs 196 u/l and 0.56 vs 0.59 mg/ml, respectively. The average of offsprings weight on 24 day postpartum in K1 and K2 was 3.48 vs 3.16kg. In conclusion, cassava plantation waste could be used as feed for pregnancy and lactating ewe. Supplementation of 30 ppm zinc is efectively increase the concentration of zinc and alkaline phosphatase in blood . Key words: cobalt, reproduction, cassava, zinc, ewe","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"29 1","pages":"91-94"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3366
Ruqiah Ganda Putri Panjaitan, M. Masriani, Z. Zulfan
This study aims to find out the eeffect of Eurycoma longifolia Jack. roots on the liver damage of pregnant mice. This research used Balb/c strain mice, 2-3 month, with the weight ranging of 27-33 gram. Samples were divided into three treatment groups consisted of three mice. Group I (KI) was negative control (placebo), only treated with 0.06 ml/20 g bw of distilled water, group II (KII) was administered with roots of Eurycoma longifolia Jack extract at dose of 14 mg/20 g bw, and group III (KIII, positive control) was administered with Schizandrae at the dose 0.06 mg/20 g bw. Treatment were done for 7 consecutive days in pregnancy period. On day 8 th , blood samples were collected to examine the level of alanine transaminase (AST) and aspartate transaminase (ALT) enzymes in serum and liver organ were collected to observe the histopathological changes. The average of ALT and AST enzymes level on K1, K2, and K3 were 18.34+0.28, 19.68+0.15, and 19.20+0.08 U/l; and 21.79+0.26, 23.42+0.41, and 22.23+0.52 U/l. The histopathological examination results showed that the administration of E. longifolia Jack roots extract and Schizandrae reveal the karyomegali and fatty change of hepatocytes. It is concluded that the administration of E. longifolia Jack roots extract at the dose of 14 mg/20 g bw could decrease the liver function of pregnant mice. Key words: Eurycoma longfolia Jack. root, pregnancy period, liver histopathological studies
本研究旨在了解长叶Eurycoma longifolia Jack的作用。对妊娠小鼠肝损伤的研究。本研究选用Balb/c品系小鼠，2-3月龄，体重27-33克。样品分为三个治疗组，每组3只小鼠。I组(KI)为阴性对照(安慰剂)，仅以0.06 ml/20 g bw的蒸馏水处理，II组(KII)以14 mg/20 g bw的剂量给药，III组(KIII)以0.06 mg/20 g bw的剂量给药。妊娠期连续治疗7 d。第8天，取血检测血清中谷丙转氨酶(AST)和天冬氨酸转氨酶(ALT)水平，取肝脏器观察组织病理变化。K1、K2和K3上ALT和AST的平均水平分别为18.34+0.28、19.68+0.15和19.20+0.08 U/l;分别为21.79+0.26、23.42+0.41、22.23+0.52 U/l。组织病理学检查结果显示，给药后肝细胞核增大，脂肪改变。综上所述，14 mg/20 g bw剂量的长叶荷叶提取物可降低妊娠小鼠肝功能。关键词:长叶芡实;根，妊娠期，肝脏组织病理学研究
{"title":"PENGARUH PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) TERHADAP KERUSAKAN ORGAN HATI MENCIT BUNTING The Effect of Eurycoma longifolia Jack. Roots on Liver Damage of Pregnant Mice","authors":"Ruqiah Ganda Putri Panjaitan, M. Masriani, Z. Zulfan","doi":"10.21157/J.KED.HEWAN.V10I1.3366","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3366","url":null,"abstract":"This study aims to find out the eeffect of Eurycoma longifolia Jack. roots on the liver damage of pregnant mice. This research used Balb/c strain mice, 2-3 month, with the weight ranging of 27-33 gram. Samples were divided into three treatment groups consisted of three mice. Group I (KI) was negative control (placebo), only treated with 0.06 ml/20 g bw of distilled water, group II (KII) was administered with roots of Eurycoma longifolia Jack extract at dose of 14 mg/20 g bw, and group III (KIII, positive control) was administered with Schizandrae at the dose 0.06 mg/20 g bw. Treatment were done for 7 consecutive days in pregnancy period. On day 8 th , blood samples were collected to examine the level of alanine transaminase (AST) and aspartate transaminase (ALT) enzymes in serum and liver organ were collected to observe the histopathological changes. The average of ALT and AST enzymes level on K1, K2, and K3 were 18.34+0.28, 19.68+0.15, and 19.20+0.08 U/l; and 21.79+0.26, 23.42+0.41, and 22.23+0.52 U/l. The histopathological examination results showed that the administration of E. longifolia Jack roots extract and Schizandrae reveal the karyomegali and fatty change of hepatocytes. It is concluded that the administration of E. longifolia Jack roots extract at the dose of 14 mg/20 g bw could decrease the liver function of pregnant mice. Key words: Eurycoma longfolia Jack. root, pregnancy period, liver histopathological studies","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"28-31"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3370
E. M. Kaiin, I. Djuwita
The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM β-mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM β-mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into culture medium resulted in 12.97% newborn cells in fibroblast culture medium passage I. Newborn rat neuron conditioned medium in fibroblast culture medium resulted 12.97% neuron cells at passage 1. The percentage was increased (14.60%) when β- mercaptoethanol added into medium. The same result was found at passage 3 (12.67%; 13.17%). It showed that fibroblast cells has potency to transdifferentiated into neuron cells when cultured with NBRN CM. Further research is needed to know the fibroblast transdifferentiation potency . Key words: fibroblast, transdifferentiation, conditioned medium, neuron cells, in vitro
本研究的目的是检测成纤维细胞在体外转分化为神经元细胞的效力。新生大鼠神经元条件培养基(NBRN CM)取材于经mDMEM不加血清培养48小时的神经元细胞。从经胰蛋白酶处理的胎鼠肌肉中收集成纤维细胞。用3种培养基培养成纤维细胞:mDMEM + 0.01 mM β-巯基乙醇;mDMEM + 50% NBRN-CM和mDMEM + 0.01 mM β-巯基乙醇+ 50% NBRN CM。作为对照，细胞用mDMEM +10%新生牛犊血清(NBCS)培养。在培养液中加入NBRN CM，成纤维细胞培养液传代1，新生大鼠神经元条件培养基在成纤维细胞培养液传代1时产生12.97%的新生细胞。在培养基中加入β-巯基乙醇后，该比例提高了14.60%。在第3次实验中也发现了同样的结果(12.67%;13.17%)。结果表明，NBRN CM培养后成纤维细胞具有向神经元细胞转分化的能力。成纤维细胞的转分化能力有待进一步研究。关键词:成纤维细胞;转分化;条件培养基
{"title":"POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro)","authors":"E. M. Kaiin, I. Djuwita","doi":"10.21157/J.KED.HEWAN.V10I1.3370","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3370","url":null,"abstract":"The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM β-mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM β-mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into culture medium resulted in 12.97% newborn cells in fibroblast culture medium passage I. Newborn rat neuron conditioned medium in fibroblast culture medium resulted 12.97% neuron cells at passage 1. The percentage was increased (14.60%) when β- mercaptoethanol added into medium. The same result was found at passage 3 (12.67%; 13.17%). It showed that fibroblast cells has potency to transdifferentiated into neuron cells when cultured with NBRN CM. Further research is needed to know the fibroblast transdifferentiation potency . Key words: fibroblast, transdifferentiation, conditioned medium, neuron cells, in vitro","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3360
Khusnan Khusnan, Dwi Kusmanto, Mitra Slipranata
The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding of methicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakarta were used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibiotics revealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA. Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA
{"title":"RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta)","authors":"Khusnan Khusnan, Dwi Kusmanto, Mitra Slipranata","doi":"10.21157/J.KED.HEWAN.V10I1.3360","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3360","url":null,"abstract":"The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding of methicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakarta were used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibiotics revealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA. Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"13-18"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3359
I. Besung, N. Astawa, K. Suata, N. K. Suwiti
This study aims to determine the relationships between macrophage activities and interleukin-6 (IL-6 levels), and titer antibody to Salmonella typhi (S. typhi). A total of 32 mice were infected intraperitoneally with 10 5 cells/ml of S. typhi. The activities of macrophages were observed a day after the infection, while the observations of the levels of IL-6 and titer antibody employing enzyme-linked immunosorbent assay (ELISA) method were conducted after one and two weeks of infection. The results showed that the average activities of macrophages after infected by S. typhi was 77.84±11.23 sel, and the levels of IL-6 and antibody titers were higher (159.55±90.11 pg and 56.87±23.32 IU respectively). The macrophage activities were significantly increased (P<0.01) with increasing the level of IL-6 to Y= -0.317x 2 + 0.0033x 2 +494.09 with the relatedness (r)= 0.89 and the antibody titers to Y= -0.020x 2 + 0.00031x + 29.083 with the relatedness (r)= 0.95 . Key words: macrophage activity, IL-6, antibody titers, Salmonella typhi
{"title":"HUBUNGAN ANTARA AKTIVASI MAKROFAG DENGAN KADAR INTERLEUKIN-6 DAN ANTIBODI TERHADAP Salmonella typhi PADA MENCIT (Relationship between the Macrophage Activity with Interleukin-6 Levels and Titers of Antibodies against Salmonella typhi)","authors":"I. Besung, N. Astawa, K. Suata, N. K. Suwiti","doi":"10.21157/J.KED.HEWAN.V10I1.3359","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3359","url":null,"abstract":"This study aims to determine the relationships between macrophage activities and interleukin-6 (IL-6 levels), and titer antibody to Salmonella typhi (S. typhi). A total of 32 mice were infected intraperitoneally with 10 5 cells/ml of S. typhi. The activities of macrophages were observed a day after the infection, while the observations of the levels of IL-6 and titer antibody employing enzyme-linked immunosorbent assay (ELISA) method were conducted after one and two weeks of infection. The results showed that the average activities of macrophages after infected by S. typhi was 77.84±11.23 sel, and the levels of IL-6 and antibody titers were higher (159.55±90.11 pg and 56.87±23.32 IU respectively). The macrophage activities were significantly increased (P<0.01) with increasing the level of IL-6 to Y= -0.317x 2 + 0.0033x 2 +494.09 with the relatedness (r)= 0.89 and the antibody titers to Y= -0.020x 2 + 0.00031x + 29.083 with the relatedness (r)= 0.95 . Key words: macrophage activity, IL-6, antibody titers, Salmonella typhi","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-15DOI: 10.21157/J.KED.HEWAN.V10I1.3367
Gholib G, S. Wahyuni, Okta Hilda Kadar, M. Adam, T. M. Lubis, Azhar A, M. Akmal, T. Siregar, T. Armansyah, T. P. Nugraha
This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) in analytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (>2 years), six kids ( 2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat
{"title":"MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)","authors":"Gholib G, S. Wahyuni, Okta Hilda Kadar, M. Adam, T. M. Lubis, Azhar A, M. Akmal, T. Siregar, T. Armansyah, T. P. Nugraha","doi":"10.21157/J.KED.HEWAN.V10I1.3367","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3367","url":null,"abstract":"This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) in analytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (>2 years), six kids ( 2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"32-36"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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