Pub Date : 2016-03-02DOI: 10.21157/J.KED.HEWAN.V10I1.3396
S. Wahyuni, S. Agungpriyono, I. K. M. Adnyane, H. Hamny, M. Jalaluddin, G. Gholib, M. Akmal, M. Adam, D. Aliza, T. Siregar
The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
{"title":"STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)","authors":"S. Wahyuni, S. Agungpriyono, I. K. M. Adnyane, H. Hamny, M. Jalaluddin, G. Gholib, M. Akmal, M. Adam, D. Aliza, T. Siregar","doi":"10.21157/J.KED.HEWAN.V10I1.3396","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3396","url":null,"abstract":"The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"82-85"},"PeriodicalIF":0.0,"publicationDate":"2016-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-21DOI: 10.21157/J.KED.HEWAN.V1I2.3123
H. Wijayanto, T. Pangestiningsih, E. Rahmi
The study was conducted to investigate the effects of caffeine treatment during organogenesis period to the fetal birth weight, using rat (Rattus norvegicus) as the animal model. Thirty-six primipara rat obtained from Unit Pengembangan Hewan Percobaan, Gadjah Mada University (UPHP-GMU), 3 month old, 165-200 g body weight, were divided into 6 groups, consisted of 6 rats each. Six of the rats have been selected based on the estrous cycles, and only rat with regular estrous were use for the experiment. The rat then were mated, and during day 6-14 of the pregnancies were treated orally with caffeine diluted in aquadest in dosage: placebo (1 cc aquadest) for group I (control), and 5.4, 10.8, 16.2, 21.6, and 27 g/200 g body weight/day for treatment groups II-VI respectively. The pregnant rat body weights were determined at day 6 of pregnancies for calculating the caffeine treatment dosages. At day 20 th of the pregnancies all of the pregnant rats were caesarotomized, and all of the fetuses were removed and weighed. The results showed that all of the treatment groups have significantly lower birth weight compare to the groups control group. More over, fetal obtained from the treatment groups also showed serious subcutaneous hemorrhagic. Keywords: organogenesis, Rattus norvegicus, birth weight
本研究以褐家鼠(Rattus norvegicus)为动物模型，探讨了器官发生期咖啡因处理对胎儿出生体重的影响。选用Gadjah Mada大学penembangan Hewan Percobaan单位的36只初产大鼠，3月龄，体重165 ~ 200g，随机分为6组，每组6只。根据发情周期选取6只大鼠，只选用发情规律的大鼠进行实验。然后对大鼠进行交配，在怀孕的第6-14天内口服经aquadest稀释的咖啡因:对照组为安慰剂(1毫升aquadest)，治疗组II-VI分别为5.4、10.8、16.2、21.6和27 g/200 g体重/天。在怀孕第6天测定怀孕大鼠的体重，以计算咖啡因的剂量。妊娠第20天，对所有妊娠大鼠进行剖宫产，取胎称重。结果显示，与对照组相比，所有治疗组的出生体重均显著降低。此外，治疗组胎儿也出现严重的皮下出血。关键词:器官发生，褐家鼠，初生重
{"title":"The Effect of Caffeine Treatment during Organogenesis Period on the Birth Weight of the Rat Fetuses (Rattus norvegicus)","authors":"H. Wijayanto, T. Pangestiningsih, E. Rahmi","doi":"10.21157/J.KED.HEWAN.V1I2.3123","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V1I2.3123","url":null,"abstract":"The study was conducted to investigate the effects of caffeine treatment during organogenesis period to the fetal birth weight, using rat (Rattus norvegicus) as the animal model. Thirty-six primipara rat obtained from Unit Pengembangan Hewan Percobaan, Gadjah Mada University (UPHP-GMU), 3 month old, 165-200 g body weight, were divided into 6 groups, consisted of 6 rats each. Six of the rats have been selected based on the estrous cycles, and only rat with regular estrous were use for the experiment. The rat then were mated, and during day 6-14 of the pregnancies were treated orally with caffeine diluted in aquadest in dosage: placebo (1 cc aquadest) for group I (control), and 5.4, 10.8, 16.2, 21.6, and 27 g/200 g body weight/day for treatment groups II-VI respectively. The pregnant rat body weights were determined at day 6 of pregnancies for calculating the caffeine treatment dosages. At day 20 th of the pregnancies all of the pregnant rats were caesarotomized, and all of the fetuses were removed and weighed. The results showed that all of the treatment groups have significantly lower birth weight compare to the groups control group. More over, fetal obtained from the treatment groups also showed serious subcutaneous hemorrhagic. Keywords: organogenesis, Rattus norvegicus, birth weight","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67659348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-21DOI: 10.21157/J.KED.HEWAN.V3I2.3104
Ummu Balqis, D. Darmawi, M. Hambal, R. Tiuria
The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%). Keywords: Ascaridia galli, embrionated eggs
{"title":"The Development of Ascaridia galli Infective Eggs by In Vitro Culture","authors":"Ummu Balqis, D. Darmawi, M. Hambal, R. Tiuria","doi":"10.21157/J.KED.HEWAN.V3I2.3104","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V3I2.3104","url":null,"abstract":"The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%). Keywords: Ascaridia galli, embrionated eggs","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67660028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-21DOI: 10.21157/J.KED.HEWAN.V3I1.3082
S. Sugito, M. Delima
Increasing in ambient temperature inside the cage could lead to heat stress in broilers. This research was conducted to find out effects of heat stress on body weight gain, heterophile-lymphocite ratio and body temperature in chicken broiler. Twenty broilers aged 20 days (strain Cobb) were randomly divided into 2 groups. The first group was treated with no heat stress, the second one was caged in 33±1 0 C temperature for 4 hours per day for 14 days. The results indicated that heat stress reduced body weight gain, increased body temperature, and changed behavior, but no effect on feed conversion ratio (FCR) and heterophile-lymphocyte ratio. It suggested that the heat stress caused detrimental effects on broiler chicken.
{"title":"Effect of Heat Stress on Body Weight Gain, Heterophile-Lymphocite Ratio and Body Temperature in Broiler","authors":"S. Sugito, M. Delima","doi":"10.21157/J.KED.HEWAN.V3I1.3082","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V3I1.3082","url":null,"abstract":"Increasing in ambient temperature inside the cage could lead to heat stress in broilers. This research was conducted to find out effects of heat stress on body weight gain, heterophile-lymphocite ratio and body temperature in chicken broiler. Twenty broilers aged 20 days (strain Cobb) were randomly divided into 2 groups. The first group was treated with no heat stress, the second one was caged in 33±1 0 C temperature for 4 hours per day for 14 days. The results indicated that heat stress reduced body weight gain, increased body temperature, and changed behavior, but no effect on feed conversion ratio (FCR) and heterophile-lymphocyte ratio. It suggested that the heat stress caused detrimental effects on broiler chicken.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67659770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-01-21DOI: 10.21157/J.KED.HEWAN.V3I1.3080
S. Novelina, E. Evalina, A. Satyaningtijas, S. Agungpriyono, H. Setijanto
This study was conducted with aim to tend out morphology of oesophagus and stomach of the cave swiflets (Collocalia linchi) at macroscopic and microscopic levels. The data revealed that esophagus was 2.93 cm in length and possessed no crop. The stomach was small and the isthmus was not clear. Mucosa of the esophagus was lined by stratified squoamus epithelium. Esophageal gland was mucous type. The glands were well developed and distributed along the esophagus. The external muscle layer consisted of inner circular and outer longitudinal layers. The stomach could be distinguished into proventriculus and ventriculus with no clear isthmus between them. The mucosa of proventriculus was lined by single columnar epithelium. The ventricular gland area was divided into cardiac, fundic and pyloric gland areas. The surface of ventriculus was lined with cuticula. In general the esophagus and stomach of the cave swiftlets were simple with no crop in the esophagus and isthmus in the stomach. These might be related with the kind of food and feeding behavior of this species.
{"title":"Morphological Study of the Oesophagus and Stomach of the Cave Swiflets (Collocalia linchi)","authors":"S. Novelina, E. Evalina, A. Satyaningtijas, S. Agungpriyono, H. Setijanto","doi":"10.21157/J.KED.HEWAN.V3I1.3080","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V3I1.3080","url":null,"abstract":"This study was conducted with aim to tend out morphology of oesophagus and stomach of the cave swiflets (Collocalia linchi) at macroscopic and microscopic levels. The data revealed that esophagus was 2.93 cm in length and possessed no crop. The stomach was small and the isthmus was not clear. Mucosa of the esophagus was lined by stratified squoamus epithelium. Esophageal gland was mucous type. The glands were well developed and distributed along the esophagus. The external muscle layer consisted of inner circular and outer longitudinal layers. The stomach could be distinguished into proventriculus and ventriculus with no clear isthmus between them. The mucosa of proventriculus was lined by single columnar epithelium. The ventricular gland area was divided into cardiac, fundic and pyloric gland areas. The surface of ventriculus was lined with cuticula. In general the esophagus and stomach of the cave swiftlets were simple with no crop in the esophagus and isthmus in the stomach. These might be related with the kind of food and feeding behavior of this species.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67659694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-22DOI: 10.21157/j.ked.hewan.v9i2.2802
Maxs Urias Ebenhaizar Sanam, Widya Asmara, Agnesia Endang Tri Hastuti Wahyuni, Michael Haryadi Wibowo, Rahmat Setya Adji
Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.
{"title":"EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX","authors":"Maxs Urias Ebenhaizar Sanam, Widya Asmara, Agnesia Endang Tri Hastuti Wahyuni, Michael Haryadi Wibowo, Rahmat Setya Adji","doi":"10.21157/j.ked.hewan.v9i2.2802","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v9i2.2802","url":null,"abstract":"Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67669478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-13DOI: 10.21157/J.KED.HEWAN.V8I2.2625
W. Suwito, W. S. Nugroho, Aeth Wahyuni, Bambang Sumiarto
Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total koliform masing-masing adalah 1,65x10 3 ; 5,75x10 3 ; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari ambing masih layak konsumsi.
{"title":"ANALISIS MIKROBIOLOGI SUSU KAMBING PERANAKAN ETTAWA (PE) DARI KABUPATEN SLEMAN YOGYAKARTA","authors":"W. Suwito, W. S. Nugroho, Aeth Wahyuni, Bambang Sumiarto","doi":"10.21157/J.KED.HEWAN.V8I2.2625","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V8I2.2625","url":null,"abstract":"Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini \u0000digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., \u0000jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total \u0000koliform masing-masing adalah 1,65x10 \u00003 \u0000; 5,75x10 \u00003 \u0000; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar \u0000Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, \u0000sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari \u0000ambing masih layak konsumsi.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67667637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-13DOI: 10.21157/J.KED.HEWAN.V8I2.2620
Tongku Nizwan Siregar, Muslim Akmal, Sri Wahyuni, H. Tarigan, Mulyadi M, Idawati Nasution
Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak vesikula seminalis terhadap kualitas spermatozoa dan konsentrasi testosteron tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 20 ekor tikus putih jantan dewasa, galur Wistar, berumur 3 -4 bulan, berat badan 250-300 g dan dibagi menjadi 4 kelompok (K1, K2, K3, dan K4), masing-masing kelompok berturut-turut diberikan 0,2 ml NaCl fisiologis, 25 μg cloprostenol, 0,2 ml ekstrak vesikula seminalis (EVS), dan 0,4 ml EVS secara intraperitoneal. Pada akhir perlakuan, seluruh tikus dikorbankan secara dislocatio cervicalis. Selanjutnya, kauda duktus epididimis dinekropsi untuk dikoleksi spermatozoanya. Pemeriksaan kualitas spermatozoa, meliputi motilitas dan konsentrasi spermatozoa. Pemeriksaan konsentrasi testosteron serum darah dilakukan menggunakan teknik enzymelinked immunosorbant assay (ELISA). Motilitas dan konsentrasi (x10 6 ) spermatozoa pada kelompok K1; K2; K3; dan K4 masingmasing adalah 3,00±0,00; 3,20±0,28; 2,00±0,86; dan 3,20±0,28 dan 146,60±71,90; 187,80±60,80; 124,20±64,70; dan 129,40±27,07. Konsentrasi testosteron pada kelompok K1; K2; K3; dan K4 masing-masing adalah 5,53+1,75; 4,68+4,56; 2,51+1,33; dan 2,40+1,60 ng/ml. Hasil penelitian menunjukkan bahwa pemberian EVS mampu meningkatkan motilitas spermatozoa tetapi tidak memengaruhi konsentrasi spermatozoa dan testosteron serum darah tikus putih.
{"title":"PEMBERIAN EKSTRAK VESIKULA SEMINALIS MENINGKATKAN KUALITAS SPERMATOZOA TETAPI TIDAK MEMENGARUHI KONSENTRASI SPERMATOZOA DAN TESTOSTERON TIKUS PUTIH (Rattus norvegicus)","authors":"Tongku Nizwan Siregar, Muslim Akmal, Sri Wahyuni, H. Tarigan, Mulyadi M, Idawati Nasution","doi":"10.21157/J.KED.HEWAN.V8I2.2620","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V8I2.2620","url":null,"abstract":"Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak vesikula seminalis terhadap kualitas spermatozoa dan konsentrasi \u0000testosteron tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 20 ekor tikus putih jantan dewasa, galur Wistar, berumur 3 -4 bulan, \u0000berat badan 250-300 g dan dibagi menjadi 4 kelompok (K1, K2, K3, dan K4), masing-masing kelompok berturut-turut diberikan 0,2 ml NaCl \u0000fisiologis, 25 μg cloprostenol, 0,2 ml ekstrak vesikula seminalis (EVS), dan 0,4 ml EVS secara intraperitoneal. Pada akhir perlakuan, seluruh \u0000tikus dikorbankan secara dislocatio cervicalis. Selanjutnya, kauda duktus epididimis dinekropsi untuk dikoleksi spermatozoanya. Pemeriksaan \u0000kualitas spermatozoa, meliputi motilitas dan konsentrasi spermatozoa. Pemeriksaan konsentrasi testosteron serum darah dilakukan menggunakan \u0000teknik enzymelinked immunosorbant assay (ELISA). Motilitas dan konsentrasi (x10 \u00006 \u0000) spermatozoa pada kelompok K1; K2; K3; dan K4 masingmasing adalah 3,00±0,00; 3,20±0,28; 2,00±0,86; dan 3,20±0,28 dan 146,60±71,90; 187,80±60,80; 124,20±64,70; dan 129,40±27,07. Konsentrasi \u0000testosteron pada kelompok K1; K2; K3; dan K4 masing-masing adalah 5,53+1,75; 4,68+4,56; 2,51+1,33; dan 2,40+1,60 ng/ml. Hasil penelitian \u0000menunjukkan bahwa pemberian EVS mampu meningkatkan motilitas spermatozoa tetapi tidak memengaruhi konsentrasi spermatozoa dan \u0000testosteron serum darah tikus putih.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67667819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-13DOI: 10.21157/J.KED.HEWAN.V8I2.2636
Mudawamah M, I. Retnaningtyas, M. F. Wadjdi, Badriyah B, S. Susilowati, Aulanni’am A, Gatot Ciptadi
Analisis genetika menggunakan random amplified polymorphic DNA (RAPD) untuk mengetahui kemiripan genetika antara kambing peranakan Ettawa (PE) hasil inseminasi buatan (IB) dan kawin alam (KA). Hasil penelitian menunjukkan bahwa dari 20 primer OPA hanya 10 primer yang bisa digunakan untuk mengamplifikasi fragmen deoxyribonucleic acid (DNA) dalam kelompok kambing PE, yang terdiri atas 21 kambing hasil KA dan 19 kambing hasil IB. Berdasarkan profil DNA yang diperoleh dan hasil analisis dengan software numerical taxonomy and multivariate analysis system (NTSYS) disimpulkan bahwa hubungan genetika antara kambing PE hasil kawin alam dengan inseminasi buatan adalah sebesar 48% dan dikategorikan rendah.
{"title":"ANALISIS KEMIRIPAN GENETIKA ANTARA KAMBING PERANAKAN ETTAWA HASIL KAWIN ALAM DENGAN INSEMINASI BUATAN BERDASARKAN RAPD","authors":"Mudawamah M, I. Retnaningtyas, M. F. Wadjdi, Badriyah B, S. Susilowati, Aulanni’am A, Gatot Ciptadi","doi":"10.21157/J.KED.HEWAN.V8I2.2636","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V8I2.2636","url":null,"abstract":"Analisis genetika menggunakan random amplified polymorphic DNA (RAPD) untuk mengetahui kemiripan genetika antara kambing \u0000peranakan Ettawa (PE) hasil inseminasi buatan (IB) dan kawin alam (KA). Hasil penelitian menunjukkan bahwa dari 20 primer OPA hanya 10 \u0000primer yang bisa digunakan untuk mengamplifikasi fragmen deoxyribonucleic acid (DNA) dalam kelompok kambing PE, yang terdiri atas 21 \u0000kambing hasil KA dan 19 kambing hasil IB. Berdasarkan profil DNA yang diperoleh dan hasil analisis dengan software numerical taxonomy and \u0000multivariate analysis system (NTSYS) disimpulkan bahwa hubungan genetika antara kambing PE hasil kawin alam dengan inseminasi buatan \u0000adalah sebesar 48% dan dikategorikan rendah.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67668369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-09-13DOI: 10.21157/J.KED.HEWAN.V8I2.2623
Hamny Hamny, Srihadi Agungpriyono, Ita Djuwita, Sri Wahyuni, W. Prasetyaningtyas, Idawati Nasution, Savitri Novelina
Penelitian ini bertujuan mengetahui distribusi glycoconjugate yang terekspresi pada sel epitelium oviduk kancil (Tragulus javanicus). Dalam penelitian ini digunakan satu oviduk kancil yang berasal dari satu ekor kancil b etina dewasa berumur lebih dari satu tahun. Sampel difiksasi dengan larutan Bouin dan diproses menurut standar histologi sampai menjadi blok parafin dan dipotong dengan ketebalan 5 µm. Jenis lektin yang digunakan adalah biotinylated (Con A, PNA, RCA, UEA I, dan WGA) dengan dosis masing-masing sebanyak 15 µg/ml. Hasil penelitian diketahui bahwa glycoconjugate dengan residu gula galaktosa, glukosa, manosa, N-asetilgalaktosamin, N-asetilglukosamin, fukosa, dan asam sialat ditemukan pada bagian apikal sel epitel dan di dalam sitoplasma. Glycoconjugate dengan residu gula N-asetilgalaktosamin merupakan glycoconjugate yang paling banyak ditemukan di bagian apikal sel epitel dan di dalam sitoplasma dibandingkan dengan glycoconjugate dengan residu gula lainnya.
{"title":"SEBARAN GLYCOCONJUGATE PADA SEL EPITEL OVIDUK KANCIL (Tragulus javanicus)","authors":"Hamny Hamny, Srihadi Agungpriyono, Ita Djuwita, Sri Wahyuni, W. Prasetyaningtyas, Idawati Nasution, Savitri Novelina","doi":"10.21157/J.KED.HEWAN.V8I2.2623","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V8I2.2623","url":null,"abstract":"Penelitian ini bertujuan mengetahui distribusi glycoconjugate yang terekspresi pada sel epitelium oviduk kancil (Tragulus javanicus). Dalam \u0000penelitian ini digunakan satu oviduk kancil yang berasal dari satu ekor kancil b etina dewasa berumur lebih dari satu tahun. Sampel difiksasi \u0000dengan larutan Bouin dan diproses menurut standar histologi sampai menjadi blok parafin dan dipotong dengan ketebalan 5 µm. Jenis lektin yang \u0000digunakan adalah biotinylated (Con A, PNA, RCA, UEA I, dan WGA) dengan dosis masing-masing sebanyak 15 µg/ml. Hasil penelitian \u0000diketahui bahwa glycoconjugate dengan residu gula galaktosa, glukosa, manosa, N-asetilgalaktosamin, N-asetilglukosamin, fukosa, dan asam \u0000sialat ditemukan pada bagian apikal sel epitel dan di dalam sitoplasma. Glycoconjugate dengan residu gula N-asetilgalaktosamin merupakan \u0000glycoconjugate yang paling banyak ditemukan di bagian apikal sel epitel dan di dalam sitoplasma dibandingkan dengan glycoconjugate dengan \u0000residu gula lainnya.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67667871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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