There are many immunological methods available for viruses detection. These methods depend on the quality and purity of antibody used in immunodetection. Availability of purified antigen, free from plant debris, will lead to produce highly purified antibody. The purified antibody in immunodetection methods will increase the sensitivity, specificity and accuracy for virus detection. Cucumber Mosaic Virus (CMV) has worldwide distribution and wide host range. Therefore the presence of highly purified antibody will help us in rapid CMV-immunodetection. The main goal in this study is to compare serologically the sensitivity and accuracy of serological methods by using two antibodies. The first, raised against the recombinant expressed coat protein (Ab/CP) and the second produced against the viral particals (Ab/V) of Ban-CMV. ELISA, Dot blot analysis and Western blotting were used as immunodetection methods. In the three serological methods, both IgGs were able to recognize the presence of Ban-CMV in the tested samples at a dilution I mg/ml. However, Western and dot blot analysis were faster in detection of Ban-CMV than ELISA using Ab/CP.
{"title":"IMMUNOLOGICAL COMPARISONS OF POLYCLONAL ANTI-BODIES PRODUCED AGAINST THE RECOMBINANT COAT PROTEIN AND VIRAL PARTICLES FOR DETECTING EGYPTIAN BANANA- CUCUMBER MOSAIC CUCUMOVIRUS","authors":"H. N. El-Din","doi":"10.21608/EJGC.2013.9966","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9966","url":null,"abstract":"There are many immunological methods available for viruses detection. These methods depend on the quality and purity of antibody used in immunodetection. Availability of purified antigen, free from plant debris, will lead to produce highly purified antibody. The purified antibody in immunodetection methods will increase the sensitivity, specificity and accuracy for virus detection. Cucumber Mosaic Virus (CMV) has worldwide distribution and wide host range. Therefore the presence of highly purified antibody will help us in rapid CMV-immunodetection. The main goal in this study is to compare serologically the sensitivity and accuracy of serological methods by using two antibodies. The first, raised against the recombinant expressed coat protein (Ab/CP) and the second produced against the viral particals (Ab/V) of Ban-CMV. ELISA, Dot blot analysis and Western blotting were used as immunodetection methods. In the three serological methods, both IgGs were able to recognize the presence of Ban-CMV in the tested samples at a dilution I mg/ml. However, Western and dot blot analysis were faster in detection of Ban-CMV than ELISA using Ab/CP.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"9 1","pages":"259-267"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-01DOI: 10.21608/EJGC.2013.10193
O. Galal, A. Zedan
The present study was conducted to determine the protective effect of parsley (leaves extract, fresh leaves, seeds and oil) on rat injected intraperitoneally with gentamicin at a dose of 10 mg/kg b.wt. The toxic effects of gentamicin were assessed in term of the changes in relative organs weights in addition the genetic variation in DNA-RAPD profiles in both liver and spleen. Results showed that no significant differences were found between the negative control and all of tested groups for relative liver weights, while the group fed on parsley oil recorded the highest value of relative spleen weight which did not differ than negative control group. For RAPD profiles, the differences in RAPD patterns refer to band intensity, loss of normal bands and appearance of new bands as compared with the negative control group. All these differences were well represented in the patterns produced in gentamicin group as well as all rat groups fed on parsley. Changes in bands intensity which were major event increased by gentamicin effect, were decreased in all groups fed on parsley forms except the group fed on seeds (for liver DNA) and the group fed on parsley leaves extract (for spleen DNA) were increased. Parsley leaves and oil (for liver) and parsley oil (for spleen) exhibited the best effect in reducing the increase in band intensity. All forms of parsley caused a decrease in the genomic template stability (%) values compared to gentamicin group. The major decrease was for leaves extract with regard to liver DNA and for seeds with regard to spleen DNA. Results of this study demonstrated the genotoxic effects of gentamicin on rat's liver and spleen. In addition, the results revealed that parsley oil may be the effective form in reducing gentamicin genotoxic effect followed by parsley fresh leaves.
{"title":"EVALUATION OF PARSLEY EFFECT AGAINST GENTAMICIN GENOTOXICITY IN RATS USING RAPD ANALYSIS","authors":"O. Galal, A. Zedan","doi":"10.21608/EJGC.2013.10193","DOIUrl":"https://doi.org/10.21608/EJGC.2013.10193","url":null,"abstract":"The present study was conducted to determine the protective effect of parsley (leaves extract, fresh leaves, seeds and oil) on rat injected intraperitoneally with gentamicin at a dose of 10 mg/kg b.wt. The toxic effects of gentamicin were assessed in term of the changes in relative organs weights in addition the genetic variation in DNA-RAPD profiles in both liver and spleen. Results showed that no significant differences were found between the negative control and all of tested groups for relative liver weights, while the group fed on parsley oil recorded the highest value of relative spleen weight which did not differ than negative control group. For RAPD profiles, the differences in RAPD patterns refer to band intensity, loss of normal bands and appearance of new bands as compared with the negative control group. All these differences were well represented in the patterns produced in gentamicin group as well as all rat groups fed on parsley. Changes in bands intensity which were major event increased by gentamicin effect, were decreased in all groups fed on parsley forms except the group fed on seeds (for liver DNA) and the group fed on parsley leaves extract (for spleen DNA) were increased. Parsley leaves and oil (for liver) and parsley oil (for spleen) exhibited the best effect in reducing the increase in band intensity. All forms of parsley caused a decrease in the genomic template stability (%) values compared to gentamicin group. The major decrease was for leaves extract with regard to liver DNA and for seeds with regard to spleen DNA. Results of this study demonstrated the genotoxic effects of gentamicin on rat's liver and spleen. In addition, the results revealed that parsley oil may be the effective form in reducing gentamicin genotoxic effect followed by parsley fresh leaves.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"379-392"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68476860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phospholipasase Cs (PLCs) has been recognized as important enzymes for their roles in regulation, signal transduction and membrane composition. Two full length wheat PLCs cDNAs, Ta-PG-PLC2-1 and Ta-PG-PLC2-2 from wheat, Triticum aestivum L., were sequenced and mapped to wheat chromosome arms using PCR amplification in diploid wheat ancestors, Triticum urartu, Aegilops speltoides, Aegilops tauschii and cytogenetic stocks of Triticum aestivum and found to have very high sequence similarity and one, PG-PLC2-1, was found to contain a frame shift mutation 29 nt downstream of the first ATG start codon. This sequence was used to survey the prevalence of the mutation in twenty one wheat cultivars and was found to be present in all wheat cultivars tested and in the ancestral diploid species, Triticum urartu.
{"title":"GENE MAPPING OF PHOSPHOGLYCEROL PHOSPHOLIPASE C HOMEOLOGS TO SINGLE CHROMOSOMES IN WHEAT (Triticum aestivum L.)","authors":"N. Sabry, F. Abdel-Tawab, P. Gulick","doi":"10.21608/EJGC.2013.9973","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9973","url":null,"abstract":"Phospholipasase Cs (PLCs) has been recognized as important enzymes for their roles in regulation, signal transduction and membrane composition. Two full length wheat PLCs cDNAs, Ta-PG-PLC2-1 and Ta-PG-PLC2-2 from wheat, Triticum aestivum L., were sequenced and mapped to wheat chromosome arms using PCR amplification in diploid wheat ancestors, Triticum urartu, Aegilops speltoides, Aegilops tauschii and cytogenetic stocks of Triticum aestivum and found to have very high sequence similarity and one, PG-PLC2-1, was found to contain a frame shift mutation 29 nt downstream of the first ATG start codon. This sequence was used to survey the prevalence of the mutation in twenty one wheat cultivars and was found to be present in all wheat cultivars tested and in the ancestral diploid species, Triticum urartu.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"333-344"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genetic variation and phylogenetic relationships among four types of yellow maize (Zea mays L.); dent, flint, sweet and pop corn, and their wild relative; teosinte (Zea mexicana), were assessed using isozymes and random amplified polymorphic DNA (RAPD) markers. The results indicated that the percentage of polymorphic loci; for peroxidase and esterase isozymes, were 66.67% and 92.59%, respectively. By applying RAPD analysis, 116 bands were obtained from seven primers with 87.07% polymorphism. The UPGMA dendrogram based on genetic distance segregated the five genotypes into two main clusters. Both isozymes and RAPD markers separated teosinte into the first cluster, whereas the four maize types were grouped together in the seconds cluster. Dent and flint types were much close to each other with high similarity indices; 0.842 and 0.792 based on peroxidase and esterase isozymes, respectively. Furthermore, the flint type closely related to sweet type in RAPD cluster (similarity index of 0.588). This high variability detected among maize types and teosinte can be used in breeding programs to maximize the use of genetic resources.
{"title":"GENETIC VARIATION AND PHYLOGENETIC RELATIONSHIPS AMONG MAIZE TYPES AND TEOSINTE AS REVEALED BY ISOZYMES AND RAPD MARKERS","authors":"O. Galal, A. Motawei, M. Farid","doi":"10.21608/EJGC.2013.9969","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9969","url":null,"abstract":"Genetic variation and phylogenetic relationships among four types of yellow maize (Zea mays L.); dent, flint, sweet and pop corn, and their wild relative; teosinte (Zea mexicana), were assessed using isozymes and random amplified polymorphic DNA (RAPD) markers. The results indicated that the percentage of polymorphic loci; for peroxidase and esterase isozymes, were 66.67% and 92.59%, respectively. By applying RAPD analysis, 116 bands were obtained from seven primers with 87.07% polymorphism. The UPGMA dendrogram based on genetic distance segregated the five genotypes into two main clusters. Both isozymes and RAPD markers separated teosinte into the first cluster, whereas the four maize types were grouped together in the seconds cluster. Dent and flint types were much close to each other with high similarity indices; 0.842 and 0.792 based on peroxidase and esterase isozymes, respectively. Furthermore, the flint type closely related to sweet type in RAPD cluster (similarity index of 0.588). This high variability detected among maize types and teosinte can be used in breeding programs to maximize the use of genetic resources.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"285-300"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-01DOI: 10.21608/EJGC.2013.10192
A. Mohammed
The vacuolar proton pump, V-ATPase, is located in the apical cell membranes of the goblet cells within the midgut of lepidopteran larvae. It plays a role in amino acid absorption, by energiz-ing the plasma membrane through pump-ing H+ ions/proton into the goblet lumen. The full transcripts of V-ATPase subunit B and C were sequenced from the midgut of pink bollworm larvae. We used RNAi to determine the efficacy of dsRNA on silencing genes encoding B and C subu-nits. Larval injection with dsRNAs target-ing subunits B and C caused larval mortal-ity of 30.77% and 25.68%, respectively.
{"title":"TARGETING THE VACUOLAR ATPase SUBUNITS B AND C IN PINK BOLLWORM, Pectinophora gossypiella (Saunders) (Lepidoptera; Gelechiidae)","authors":"A. Mohammed","doi":"10.21608/EJGC.2013.10192","DOIUrl":"https://doi.org/10.21608/EJGC.2013.10192","url":null,"abstract":"The vacuolar proton pump, V-ATPase, is located in the apical cell membranes of the goblet cells within the midgut of lepidopteran larvae. It plays a role in amino acid absorption, by energiz-ing the plasma membrane through pump-ing H+ ions/proton into the goblet lumen. The full transcripts of V-ATPase subunit B and C were sequenced from the midgut of pink bollworm larvae. We used RNAi to determine the efficacy of dsRNA on silencing genes encoding B and C subu-nits. Larval injection with dsRNAs target-ing subunits B and C caused larval mortal-ity of 30.77% and 25.68%, respectively.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"365-378"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68476760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-01DOI: 10.21608/EJGC.2013.10194
A. M. Jnied, M. Rashed, M. Sallam, A. Atta, A. E. Badawy
Three Egyptian (Baladi, Barki and Zaraibi) and one Syrian (Damascus) goat breeds were used to isolate and characterize acrosin gene, exon 5. A sample of ten bucks individuals from Zaraibi and Damascus breeds and five bucks individuals from Baladi and Barki breeds were randomly taken and DNA was extracted from each individual and analyzed with the acrosin specific primers. One band with a fragment size of a 378 bp in all individual samples for all breeds represent exon 5 of Acr gene was obtained. Sequence analysis showed that Baladi and Zaraibi breeds were identical and differ from sequences of Damascus and Barki breeds (who also identical) by one base pair number 266 (T in Baladi and Zaraibi breeds while it was C in Barki and Damascus breeds). The genetic analysis for Acr gene, exon 5 showed that this region was conserved among the studied goat breeds.
{"title":"ISOLATION AND CHARACTERIZATION OF ACROSIN GENE (Acr), EXON 5 IN THE BUCKS OF GOAT BREEDS","authors":"A. M. Jnied, M. Rashed, M. Sallam, A. Atta, A. E. Badawy","doi":"10.21608/EJGC.2013.10194","DOIUrl":"https://doi.org/10.21608/EJGC.2013.10194","url":null,"abstract":"Three Egyptian (Baladi, Barki and Zaraibi) and one Syrian (Damascus) goat breeds were used to isolate and characterize acrosin gene, exon 5. A sample of ten bucks individuals from Zaraibi and Damascus breeds and five bucks individuals from Baladi and Barki breeds were randomly taken and DNA was extracted from each individual and analyzed with the acrosin specific primers. One band with a fragment size of a 378 bp in all individual samples for all breeds represent exon 5 of Acr gene was obtained. Sequence analysis showed that Baladi and Zaraibi breeds were identical and differ from sequences of Damascus and Barki breeds (who also identical) by one base pair number 266 (T in Baladi and Zaraibi breeds while it was C in Barki and Damascus breeds). The genetic analysis for Acr gene, exon 5 showed that this region was conserved among the studied goat breeds.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"393-404"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68477000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was carried out to analysis the genetic differentiation between seven species of Cucurbitaceae by using biochemical and molecular markers. Analysis of SDS-PAGE of soluble proteins for the seven Cucurbitaceae species such as: Cucumis melo L., Citrullus lanatus L., Citrullus sativtus L., Citrullus colocynthis L., Cucumis prophetarum, Cucurbita pepo L. and Corallocarpus schempri, revealed polymorphism of 98%. Seven isozyme systems including Acp (acid phosphatase), Adh (alchol dehydrogenase), α- and β-Est (esterases), Ao (Aldehyde oxidase), Mdh (malate dehydrogenase) and Prx (peroxidase) successfully indicated genetic variability estimated as 70% polymorphism discriminating between seven Cucurbitaceae species. Six preselected RAPD primers were used in the present study to discriminating between the seven Cucurbitaceae species. RAPD markers revealed (99%) polymorphism that is considerable the highest polymorphism among seven Cucurbitaceae species and the lowest similarity (1%), ISSR markers revealed (98%) polymorphism and finally, AFLP gave (54.8%) of polymorphism with the lowest polymorphism indicating the highest similarity (46%). Based on the results and polymorphism percentages, RAPD, ISSR, SDS-PAGE of soluble proteins, isozymes and AFLP were useful molecular and biochemical tools to discriminate between the seven Cucurbitaceae species.
{"title":"GENETIC ANALYSIS IN SOME Cucurbitaceae PLANTS","authors":"R. Khalil, A. H. Hassan","doi":"10.21608/EJGC.2013.9975","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9975","url":null,"abstract":"The present study was carried out to analysis the genetic differentiation between seven species of Cucurbitaceae by using biochemical and molecular markers. Analysis of SDS-PAGE of soluble proteins for the seven Cucurbitaceae species such as: Cucumis melo L., Citrullus lanatus L., Citrullus sativtus L., Citrullus colocynthis L., Cucumis prophetarum, Cucurbita pepo L. and Corallocarpus schempri, revealed polymorphism of 98%. Seven isozyme systems including Acp (acid phosphatase), Adh (alchol dehydrogenase), α- and β-Est (esterases), Ao (Aldehyde oxidase), Mdh (malate dehydrogenase) and Prx (peroxidase) successfully indicated genetic variability estimated as 70% polymorphism discriminating between seven Cucurbitaceae species. Six preselected RAPD primers were used in the present study to discriminating between the seven Cucurbitaceae species. RAPD markers revealed (99%) polymorphism that is considerable the highest polymorphism among seven Cucurbitaceae species and the lowest similarity (1%), ISSR markers revealed (98%) polymorphism and finally, AFLP gave (54.8%) of polymorphism with the lowest polymorphism indicating the highest similarity (46%). Based on the results and polymorphism percentages, RAPD, ISSR, SDS-PAGE of soluble proteins, isozymes and AFLP were useful molecular and biochemical tools to discriminate between the seven Cucurbitaceae species.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"345-364"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New olive vegetative clone namely (Clone AO13) and two olive cultivars namely (Manzanillo and Picual) grown in the orchard of the Abou Tesht region, Qena governorate, were examined and evaluated during 2011 and 2012 seasons for some of their vegetative, flowering, fruit set characteristics and fruit properties i.e. shoot length, leaf area, flower density, inflorescence length, total number of flowers per inflorescence, sexual expression%, initial fruit set%, horticulture fruit set%, fruit drop%, fruit weight (g) and pulp/fruit%. RAPD-PCR (polymerase chain reaction randomly amplified polymorphic DNA) analysis was performed to assess the genetic variation for these characteristics between two olive cultivars and clone AO13. The obtained results showed that, the leaf area was highest in Picual cv. followed by the other two cultivars. In Manzanillo cv. flower density and total number of flowers per inflorescence were the highest values compared to the other two cultivars, but, Clone AO13 gave the highest value of the sexual expression%, especially in the first season, Clone AO13 was the highest in initial fruit set followed by the other two cultivars. On the other hand, there were no significant differences among studied cultivars in the horticulture fruit set% as well as fruit weight and pulp/fruit%. Dendrogram tree generated across RAPD analysis demonstrated that, the three tested olive cultivars in this study were highly similar at the DNA level. The highest similarity value was observed between Manzanillo and Picual cultivars (86.1%), while the lowest similarity was recorded between Manzanillo cv. and Clone AO13 (77.5%). Some RAPD markers may be linked to some flowering and fruit set characteristics such as sexual expression% (two primers A17- 650 bp and C12- 700 bp), initial fruit set% (six primers, from B12 at 180 bp to B18 at 750 bp) and fruit drop% (seven primers, from B12 at 190 bp to M02 at 800 bp)
{"title":"IDENTIFICATION OF MOLECULAR MARKERS LINKED WITH SOME HORTICULTURE CHARACTERISTICS OF SOME OLIVE (Olea europaea L.) CULTIVARS","authors":"A. El-khawaga, M. Maklad","doi":"10.21608/EJGC.2013.9971","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9971","url":null,"abstract":"New olive vegetative clone namely (Clone AO13) and two olive cultivars namely (Manzanillo and Picual) grown in the orchard of the Abou Tesht region, Qena governorate, were examined and evaluated during 2011 and 2012 seasons for some of their vegetative, flowering, fruit set characteristics and fruit properties i.e. shoot length, leaf area, flower density, inflorescence length, total number of flowers per inflorescence, sexual expression%, initial fruit set%, horticulture fruit set%, fruit drop%, fruit weight (g) and pulp/fruit%. RAPD-PCR (polymerase chain reaction randomly amplified polymorphic DNA) analysis was performed to assess the genetic variation for these characteristics between two olive cultivars and clone AO13. The obtained results showed that, the leaf area was highest in Picual cv. followed by the other two cultivars. In Manzanillo cv. flower density and total number of flowers per inflorescence were the highest values compared to the other two cultivars, but, Clone AO13 gave the highest value of the sexual expression%, especially in the first season, Clone AO13 was the highest in initial fruit set followed by the other two cultivars. On the other hand, there were no significant differences among studied cultivars in the horticulture fruit set% as well as fruit weight and pulp/fruit%. Dendrogram tree generated across RAPD analysis demonstrated that, the three tested olive cultivars in this study were highly similar at the DNA level. The highest similarity value was observed between Manzanillo and Picual cultivars (86.1%), while the lowest similarity was recorded between Manzanillo cv. and Clone AO13 (77.5%). Some RAPD markers may be linked to some flowering and fruit set characteristics such as sexual expression% (two primers A17- 650 bp and C12- 700 bp), initial fruit set% (six primers, from B12 at 180 bp to B18 at 750 bp) and fruit drop% (seven primers, from B12 at 190 bp to M02 at 800 bp)","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"319-332"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reliable, simple, and rapid detection tests are needed to better monitor and manage plant virus infection. Microsphere-based diagnostic tests can fulfill these needs and are inexpensive and portable for any laboratory. In the present study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting Citrus tristeza virus (CTV) was developed. Polyclonal antibodies were immobilized on the surface of latex beads and tested for the ability to detect CTV. Under optimized conditions, LAT revealed successful detection of the viral particles in the infected citrus plant.
{"title":"DEVELOPMENT OF LATEX AGGLUTINATION TEST FOR RAPID DETECTION OF CITRUS TRISTEZA CLOSTEROVIRUS","authors":"K. Abdalla, H. N. El-Din","doi":"10.21608/EJGC.2013.9959","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9959","url":null,"abstract":"Reliable, simple, and rapid detection tests are needed to better monitor and manage plant virus infection. Microsphere-based diagnostic tests can fulfill these needs and are inexpensive and portable for any laboratory. In the present study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting Citrus tristeza virus (CTV) was developed. Polyclonal antibodies were immobilized on the surface of latex beads and tested for the ability to detect CTV. Under optimized conditions, LAT revealed successful detection of the viral particles in the infected citrus plant.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"223-231"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68477874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Fahmy, K. E. Mangoury, Walaa Abou El-Wafa, Hoda M. S. Barakat, S. E. El-Khodary, S. Muthukrishnan
Plant diseases are caused by a variety of plant pathogens including fungi, and their management requires the use of techniques like transgenic technology and genetics. The chitinase gene, known to have a vital role in fungal disease defense, was introduced into the Egyptian wheat cv. Giza 164 via biolistic bombardment. Immature embryo derived calli were sub-cultured on CIMB medium containing bialaphos. After six weeks, the survived embryogenic calli were placed on MSRB regeneration selection medium. Finally, the green regenerated shoots produced were then transferred onto FMSB rooting selection medium. After the two weeks, healthy rooted plantlets were established under the rooting selection medium, and were then transferred into soil pots, then incubated in control growth chamber for acclimatization and subsequently trans-ferred to greenhouse until seed setting. In-tegration of the transgene with a transfor-mation frequency of 1.8% was confirmed by PCR and dot-blot analyses.
{"title":"GENETIC TRANSFORMATION OF EGYPTIAN WHEAT (Triticum aestivum L.) WITH CHITINASE GENE VIA MICROPROJECTILE BOMBARDMENT","authors":"A. Fahmy, K. E. Mangoury, Walaa Abou El-Wafa, Hoda M. S. Barakat, S. E. El-Khodary, S. Muthukrishnan","doi":"10.21608/EJGC.2013.9964","DOIUrl":"https://doi.org/10.21608/EJGC.2013.9964","url":null,"abstract":"Plant diseases are caused by a variety of plant pathogens including fungi, and their management requires the use of techniques like transgenic technology and genetics. The chitinase gene, known to have a vital role in fungal disease defense, was introduced into the Egyptian wheat cv. Giza 164 via biolistic bombardment. Immature embryo derived calli were sub-cultured on CIMB medium containing bialaphos. After six weeks, the survived embryogenic calli were placed on MSRB regeneration selection medium. Finally, the green regenerated shoots produced were then transferred onto FMSB rooting selection medium. After the two weeks, healthy rooted plantlets were established under the rooting selection medium, and were then transferred into soil pots, then incubated in control growth chamber for acclimatization and subsequently trans-ferred to greenhouse until seed setting. In-tegration of the transgene with a transfor-mation frequency of 1.8% was confirmed by PCR and dot-blot analyses.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"233-246"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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