The effect of different doses of gamma irradiation on cytogenetic and biochemical characters was studied in two Egyptian cultivars of garlic (Balady and Sids-40). Identification of DNA polymorphism among the treatments through a Randomly Amplified Polymorphic DNA (RAPD) marker analysis was also of interest in this study. The cytological analyses showed that the highest percentage value of mitotic index (MI) was recorded for cultivar Sids-40 (13.07 ± 2.04) at 1000 rad, while the dose 1250 rad exhibited the lowest value (4.65 ± 0.43) for Balady cultivar. It was of specific interest to notice that the γ-irradiation dose of 4000 rad which caused the significant decrease in MI in Sids-40 garlic cultivar gave also the highest values of chromosomal abnormalities in the same cultivar. Both the lowest and highest γ-irradiation doses used (500 and 8000 rad, respectively) caused increase in MI in both garlic cultivars. While, the 4000 rad dose caused decrease in both garlic varieties. On the other hand, all doses of gamma rays induced significant increase in the percentage of chromosomal abnormalities which examined at different mitotic stages and the most frequent aberrations were c-metaphase and fragments. Data showed that cultivar Balady had the highest values of total chromosomal abnormalities at 1250 rad, while Sids-40 showed the highest value at 4000 rad. The biochemical studies exhibited changes in protein banding patterns; these changes included alterations in number of bands, band intensity and disappearance or appearance of certain bands. The occurred changes in RAPD profiles using eight primers following γ-irradiation treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the untreated plants. These results indicated that polymorphism and genomic template stability (GTS) value was affected at the above gamma doses. In conclusion, DNA polymorphisms detected by RAPD analysis could be used as a useful biomarker assay for the detection of genotoxic effects of γ-irradiation on plants.
研究了不同剂量γ辐照对两个埃及大蒜品种(Balady和Sids-40)细胞遗传学和生化特性的影响。通过随机扩增多态性DNA (RAPD)标记分析鉴定处理之间的DNA多态性也是本研究的兴趣所在。细胞学分析表明，Sids-40在1000 rad剂量下有丝分裂指数(MI)百分比值最高(13.07±2.04)，Balady在1250 rad剂量下最低(4.65±0.43)。特别值得注意的是，在Sids-40大蒜品种中，引起MI显著降低的4000 rad γ辐照剂量也使染色体异常值在同一品种中最高。使用最低和最高γ辐射剂量(分别为500和8000 rad)均导致两个大蒜品种的心肌梗死增加。而4000 rad剂量对两种大蒜品种均有抑制作用。另一方面，在有丝分裂的不同阶段，所有剂量的伽马射线都引起染色体异常百分比的显著增加，最常见的畸变是c-中期和片段。结果表明，品种Balady在1250 rad时染色体总异常值最高，而Sids-40在4000 rad时染色体总异常值最高。这些变化包括频带数量、频带强度和某些频带消失或出现的变化。经γ辐射处理后，8种引物的RAPD谱图发生了变化，包括条带强度的变化、正常条带的缺失和新条带的出现。这些结果表明，在上述γ剂量下，多态性和基因组模板稳定性(GTS)值受到影响。综上所述，RAPD检测的DNA多态性可作为检测γ辐照对植物遗传毒性的有效生物标志物。
{"title":"DETECTION OF GENETIC EFFECTS IN γ-IRRADIATED GARLIC (Allium sativum L.) USING CYTOGENETIC, BIOCHEMICAL ANDRAPD ANALYSIS","authors":"A. Ali, A. A. Aboulila, Fatma F. Elnagar","doi":"10.21608/EJGC.2015.9718","DOIUrl":"https://doi.org/10.21608/EJGC.2015.9718","url":null,"abstract":"The effect of different doses of gamma irradiation on cytogenetic and biochemical characters was studied in two Egyptian cultivars of garlic (Balady and Sids-40). Identification of DNA polymorphism among the treatments through a Randomly Amplified Polymorphic DNA (RAPD) marker analysis was also of interest in this study. The cytological analyses showed that the highest percentage value of mitotic index (MI) was recorded for cultivar Sids-40 (13.07 ± 2.04) at 1000 rad, while the dose 1250 rad exhibited the lowest value (4.65 ± 0.43) for Balady cultivar. It was of specific interest to notice that the γ-irradiation dose of 4000 rad which caused the significant decrease in MI in Sids-40 garlic cultivar gave also the highest values of chromosomal abnormalities in the same cultivar. Both the lowest and highest γ-irradiation doses used (500 and 8000 rad, respectively) caused increase in MI in both garlic cultivars. While, the 4000 rad dose caused decrease in both garlic varieties. On the other hand, all doses of gamma rays induced significant increase in the percentage of chromosomal abnormalities which examined at different mitotic stages and the most frequent aberrations were c-metaphase and fragments. Data showed that cultivar Balady had the highest values of total chromosomal abnormalities at 1250 rad, while Sids-40 showed the highest value at 4000 rad. The biochemical studies exhibited changes in protein banding patterns; these changes included alterations in number of bands, band intensity and disappearance or appearance of certain bands. The occurred changes in RAPD profiles using eight primers following γ-irradiation treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the untreated plants. These results indicated that polymorphism and genomic template stability (GTS) value was affected at the above gamma doses. In conclusion, DNA polymorphisms detected by RAPD analysis could be used as a useful biomarker assay for the detection of genotoxic effects of γ-irradiation on plants.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"44 1","pages":"309-330"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bean rust, caused by Uromyces appendiculatus (Pers.) Unger, is an important disease in both temperate and tropical bean production regions. Twenty two genotypes of snap bean (Phaseolus vulgaris L.) were collected and screened for resistance to bean rust disease in two seasons. Four genotypes, i.e., Concessa, Hort. 440, Coby and Hana were completely free from infection and were rated as immune to this disease. While, another four genotypes were recorded to disease severity ranged from 1.68% to 3.19% and were categorized as resistant (hypersensitive). Only one genotype was categorized as resistant with disease severity of 9.7%. The remaining 13 genotypes were susceptible having a disease severity ranged from 17.39% to 57.80%. Banding pattern of ten selected genotypes with various levels of resistance and susceptibility to bean rust and genetic diversity among them was evaluated using RAPD, ISSRs and SSRs. The three molecular systems generated total of 75 bands with polymorphism ranged from 60% to 85%. The large number of polymorphic bands allowed easy identification of the different genotypes at the DNA level. The developed dendrogram divided the common bean genotypes into two main clusters. It could be concluded that new promising resources for resistance to leaf rust were recorded in Egyptian snap bean germplasm. In addition, the developed banding patterns for these newly identified valuable Egyptian common bean accessions immune and resistant to leaf rust reported herein could support the future Egyptian snap bean germplasm collection, preservation and utilization.
豆锈病，由尾尾尿霉菌引起。黑热病是温带和热带豆类产区的一种重要病害。对菜豆(Phaseolus vulgaris L.) 22个基因型进行了2个季节的抗锈病筛选。4个基因型，即Concessa、Hort. 440、Coby和Hana完全不受感染，被评为免疫。另外4个基因型的疾病严重程度从1.68%到3.19%不等，被归类为耐药(超敏感)。只有一种基因型被归类为耐药，疾病严重程度为9.7%。其余13个基因型易感，疾病严重程度为17.39% ~ 57.80%。利用RAPD、ISSRs和SSRs对10个不同程度的豆锈病抗性和敏感性基因型的带带模式和遗传多样性进行了评价。3个分子体系共产生75个条带，多态性范围为60% ~ 85%。大量的多态性条带使得在DNA水平上容易识别不同的基因型。发育的树状图将普通豆基因型分为两个主要簇。由此可见，在埃及豆荚种质中发现了新的抗叶锈病资源。此外，这些新鉴定的具有叶锈病免疫和抗性的埃及普通豆种质资源的条带模式也为未来埃及菜豆种质资源的收集、保存和利用提供了依据。
{"title":"FIELD EVALUATION AND MOLECULAR ANALYSIS OF BEAN GENOTYPES FOR RESISTANCE TO RUST DISEASE","authors":"M. El-Awady, A. Hamed","doi":"10.21608/EJGC.2015.9707","DOIUrl":"https://doi.org/10.21608/EJGC.2015.9707","url":null,"abstract":"Bean rust, caused by Uromyces appendiculatus (Pers.) Unger, is an important disease in both temperate and tropical bean production regions. Twenty two genotypes of snap bean (Phaseolus vulgaris L.) were collected and screened for resistance to bean rust disease in two seasons. Four genotypes, i.e., Concessa, Hort. 440, Coby and Hana were completely free from infection and were rated as immune to this disease. While, another four genotypes were recorded to disease severity ranged from 1.68% to 3.19% and were categorized as resistant (hypersensitive). Only one genotype was categorized as resistant with disease severity of 9.7%. The remaining 13 genotypes were susceptible having a disease severity ranged from 17.39% to 57.80%. Banding pattern of ten selected genotypes with various levels of resistance and susceptibility to bean rust and genetic diversity among them was evaluated using RAPD, ISSRs and SSRs. The three molecular systems generated total of 75 bands with polymorphism ranged from 60% to 85%. The large number of polymorphic bands allowed easy identification of the different genotypes at the DNA level. The developed dendrogram divided the common bean genotypes into two main clusters. It could be concluded that new promising resources for resistance to leaf rust were recorded in Egyptian snap bean germplasm. In addition, the developed banding patterns for these newly identified valuable Egyptian common bean accessions immune and resistant to leaf rust reported herein could support the future Egyptian snap bean germplasm collection, preservation and utilization.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"44 1","pages":"205-219"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. M. Hassan, Zaki Ahmed El-Feky, E. A. Eissa, A. Ahmed
The aim of the present study was to identify and compare the variations among clinical of P. multocida isolates originating from different hosts, sheep (n = 4), buffalo (n = 4) and cattle (n = 4). These isolates were obtained from various locations in the Fayoum governorate, Egypt and they were analyzed by morphological, API kit and RAPD PCR at molecular level to check the distribution of Pneumonia and hemorrhagic septicemia in different farm animals. The chosen isolates exhibited luxuriant growth on blood agar with translucent grayish or yellowish green colonies. However, they showed no growth on MacConkey and no hemolysis of blood; also isolates were positive for oxidase, catalase and H2S production, negative of urease and can ferment glucose, fructose and glucose but can't ferment lactose. Antibiotic sensitivity showed that isolates were sensitive to tetracycline, clindamycin and erythromycin but resistant to ampicillin, trimethoprim/sulfamethoxazole and flucloxacillin. To determine the genetic diversity nine random primers OPA-01 OPA09 were used to which generate different profiles. The distinct RAPD profiles of p. multocida strains showed specificity with regard to the host and the disease induced.
{"title":"DETERMINATION OF THE RELATIONSHIPS BETWEEN Pasteurella multocida ISOLATED FROM DIFFERENT FARM ANIMALS AND THEIR HOST RANGE CONTACTS IN EGYPT USING BIOCHEMICAL AND MOLECULAR TECHNIQUES","authors":"G. M. Hassan, Zaki Ahmed El-Feky, E. A. Eissa, A. Ahmed","doi":"10.21608/EJGC.2015.9713","DOIUrl":"https://doi.org/10.21608/EJGC.2015.9713","url":null,"abstract":"The aim of the present study was to identify and compare the variations among clinical of P. multocida isolates originating from different hosts, sheep (n = 4), buffalo (n = 4) and cattle (n = 4). These isolates were obtained from various locations in the Fayoum governorate, Egypt and they were analyzed by morphological, API kit and RAPD PCR at molecular level to check the distribution of Pneumonia and hemorrhagic septicemia in different farm animals. The chosen isolates exhibited luxuriant growth on blood agar with translucent grayish or yellowish green colonies. However, they showed no growth on MacConkey and no hemolysis of blood; also isolates were positive for oxidase, catalase and H2S production, negative of urease and can ferment glucose, fructose and glucose but can't ferment lactose. Antibiotic sensitivity showed that isolates were sensitive to tetracycline, clindamycin and erythromycin but resistant to ampicillin, trimethoprim/sulfamethoxazole and flucloxacillin. To determine the genetic diversity nine random primers OPA-01 OPA09 were used to which generate different profiles. The distinct RAPD profiles of p. multocida strains showed specificity with regard to the host and the disease induced.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"53 1","pages":"221-233"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Makhlouf, Amira A. El Kemany, A. Riad, Y. A. Abou-Youssef
Thirty three local soil samples were collected from Westward till Sewa Oasis in Egypt to search novel isolates of Bacillus thuringiensis (Bt) and evaluate their toxic potentiality to overcome the serious problem of evolved resistance by insects to the pesticidal activity. The first instars larvae of cotton leaf worm (Spodoptera littoralis) were used to test their toxic potentiality in the presence of the two standard strains kurstaki (K) and neoleonensis H24a (N). The results showed that three isolates from Alexandria (AL3, AL7 and AL11) and two isolates from Kafr - el-Dawar (KD2 and KD3) were presumptively confirmed as Bt. by morphological and microscopic characters. The treated larvae with Insecticidal crystal protein (ICP) exhibited mortality percentage around 50% for all isolates except AL3 isolate. The vegetative insecticidal proteins (VIPs) of KD3, KD2 and AL11 isolates revealed mortality percentages more than 75%. While, AL3 and AL7 isolates showed differential toxicity. Crystal proteins analysis by SDS-PAGE showed KD2, KD3 and AL11 isolates gave similar profiles as those of "K" strain which was characterized with 135 kDa and 70 kDa bands. AL3 isolate failed to show any noticeable bands. According to their plasmids patterns, 4 kb was shown in all isolates and the reference strains except AL3 isolate. Cry1, Vip1 and Vip2 genes of the isolates was detected by polymerase chain reaction (PCR). The results indicated that one band with 550bp in size was present in all isolates except the AL3 isolate. Vip1 primer succeeds to amplify a band 400 bp in size in all isolates and “K" strain. Vip2 primer failed to react with any genome of the studied isolates or reference strains. This study suggested that KD2, KD3 and AL11 isolates may lead to be identified as potential strains of Bt. for their use in the development of bioinsecticide to control insect pests in Egypt.
{"title":"ISOLATION, TOXICITY AND MOLECULAR CHARACTERIZATION OF NATIVE Bacillus thuringiensis ISOLATES FROM EGYPTIAN SOIL","authors":"A. Makhlouf, Amira A. El Kemany, A. Riad, Y. A. Abou-Youssef","doi":"10.21608/EJGC.2015.9723","DOIUrl":"https://doi.org/10.21608/EJGC.2015.9723","url":null,"abstract":"Thirty three local soil samples were collected from Westward till Sewa Oasis in Egypt to search novel isolates of Bacillus thuringiensis (Bt) and evaluate their toxic potentiality to overcome the serious problem of evolved resistance by insects to the pesticidal activity. The first instars larvae of cotton leaf worm (Spodoptera littoralis) were used to test their toxic potentiality in the presence of the two standard strains kurstaki (K) and neoleonensis H24a (N). The results showed that three isolates from Alexandria (AL3, AL7 and AL11) and two isolates from Kafr - el-Dawar (KD2 and KD3) were presumptively confirmed as Bt. by morphological and microscopic characters. The treated larvae with Insecticidal crystal protein (ICP) exhibited mortality percentage around 50% for all isolates except AL3 isolate. The vegetative insecticidal proteins (VIPs) of KD3, KD2 and AL11 isolates revealed mortality percentages more than 75%. While, AL3 and AL7 isolates showed differential toxicity. Crystal proteins analysis by SDS-PAGE showed KD2, KD3 and AL11 isolates gave similar profiles as those of \"K\" strain which was characterized with 135 kDa and 70 kDa bands. AL3 isolate failed to show any noticeable bands. According to their plasmids patterns, 4 kb was shown in all isolates and the reference strains except AL3 isolate. Cry1, Vip1 and Vip2 genes of the isolates was detected by polymerase chain reaction (PCR). The results indicated that one band with 550bp in size was present in all isolates except the AL3 isolate. Vip1 primer succeeds to amplify a band 400 bp in size in all isolates and “K\" strain. Vip2 primer failed to react with any genome of the studied isolates or reference strains. This study suggested that KD2, KD3 and AL11 isolates may lead to be identified as potential strains of Bt. for their use in the development of bioinsecticide to control insect pests in Egypt.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"44 1","pages":"405-422"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68480147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The drought-tolerant wild plant C. procera is important in medicine, industry and ornamental fields. Generally, its bark and leaves are used for many Folk medicine treatments. ATP4 (mitochondrion ATPase subunit 4), one of ATP gene family provides instructions for making transporter proteins called ATPases, which use energy from ATP molecule to move substances, such as fats, sugars, charged atoms or molecules (ions), and drugs, across the cell membranes. In this study, we uncovered and characterized ATP4 (ATP4, NCBI accession no. KP171515) gene in this medicinal plant from the de novo assembled transcriptome contigs of the high-throughput sequencing dataset. A number of GenBank accessions for ATP4 sequences were blasted with the recovered de novo assembled contigs. Homology modeling of the deduced amino acids was further carried out using Swiss-Model, accessible via the EXPASY. Superimposition of C. procera ATP4 full sequence model on Chain A, Subcomplex of the Stator of Bovine Mitochondrial ATP Synthase (PDB accession no. 2CLY_A) was constructed using RasMol and Deep-View programs. The functional domains of the novel ATP4 amino acids sequence were identified from the NCBI conserved domain data-base (CDD, accession no. cl21478) that provide insights into sequence structure/function relationships, as well as domain models imported from a number of external source databases (Pfam, SMART, COG, PRK, TIGRFAM).
{"title":"CHARACTERIZATION OF ATP GENE IN Calotropis procera MITOCHONDRIAL GENOME","authors":"S. Hassanein","doi":"10.21608/EJGC.2014.9920","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9920","url":null,"abstract":"The drought-tolerant wild plant C. procera is important in medicine, industry and ornamental fields. Generally, its bark and leaves are used for many Folk medicine treatments. ATP4 (mitochondrion ATPase subunit 4), one of ATP gene family provides instructions for making transporter proteins called ATPases, which use energy from ATP molecule to move substances, such as fats, sugars, charged atoms or molecules (ions), and drugs, across the cell membranes. In this study, we uncovered and characterized ATP4 (ATP4, NCBI accession no. KP171515) gene in this medicinal plant from the de novo assembled transcriptome contigs of the high-throughput sequencing dataset. A number of GenBank accessions for ATP4 sequences were blasted with the recovered de novo assembled contigs. Homology modeling of the deduced amino acids was further carried out using Swiss-Model, accessible via the EXPASY. Superimposition of C. procera ATP4 full sequence model on Chain A, Subcomplex of the Stator of Bovine Mitochondrial ATP Synthase (PDB accession no. 2CLY_A) was constructed using RasMol and Deep-View programs. The functional domains of the novel ATP4 amino acids sequence were identified from the NCBI conserved domain data-base (CDD, accession no. cl21478) that provide insights into sequence structure/function relationships, as well as domain models imported from a number of external source databases (Pfam, SMART, COG, PRK, TIGRFAM).","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"257-269"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The adrenergic receptor β3 (ADRβ3) is the major regulator of lipolysis and homeostasis and predominantly expressed in brown and white adipose tissues (Cannon and Nedergaard, 2004). The ADRβ3 gene which encodes for this receptor was studied as a candidate gene associated with growth traits, body dimensional measurements and conformation indices of Barki sheep. Single strand conformational polymorphism (SSCP) was used to identify the variation in the intron region of ADRβ3 gene for 136 male and female Barki lambs. Eighteen SSCP genotypes representing six alleles (A, B, C, D, E and H) were detected. The frequencies of these alleles were 0.331, 0.184, 0.283, 0.044, 0.073 and 0.084, respectively. The results indicated that ADRβ3 genotype significantly (P ˂ 0.05) affected marketing weight, thigh circumference, body mass index and skeletal muscle index; and also significantly (P ˂ 0.01) affected post-weaning daily gain. Least square means analysis showed that animals with genotypes AA, AC and CC had higher post-weaning daily gain, marketing weight, thigh circumference, body mass index and skeletal muscle index than animals with genotypes CD, CE and CH. In view of the obtained results, molecular marker selection using ADRβ3 gene is warranted to increase post-weaning growth rate, whole-body mass and skeletal muscle mass in Barki sheep and will be of considerable economic value to sheep producer.
肾上腺素能受体β3 (ADRβ3)是脂肪分解和体内平衡的主要调节因子，主要在棕色和白色脂肪组织中表达(Cannon and Nedergaard, 2004)。研究了编码该受体的ADRβ3基因作为与巴氏羊生长性状、体尺寸和构象指标相关的候选基因。采用单链构象多态性(SSCP)对136只巴尔基公羊和母羊的ADRβ3基因内含子区变异进行了分析。共检测到18个SSCP基因型，分别代表A、B、C、D、E和H 6个等位基因。等位基因频率分别为0.331、0.184、0.283、0.044、0.073和0.084。结果表明，ADRβ3基因型对市场体重、大腿围、体重指数和骨骼肌指数有显著影响(P值小于0.05);显著影响断奶后日增重(P小于0.01)。最小二乘法分析结果表明，AA、AC和CC基因型动物断奶后日增重、市场体重、大腿围、体重指数和骨骼肌指数均高于CD、CE和CH基因型动物。由此可见，利用ADRβ3基因选择分子标记可以提高巴基羊断奶后的生长率、全身质量和骨骼肌质量，对绵羊养殖者具有重要的经济价值。
{"title":"SINGLE STRAND CONFORMATIONAL POLYMORPHISM OF ADRβ3 GENE AND ITS ASSOCIATION WITH LIVE PERFOR-MANCE TRAITS IN BARKI SHEEP","authors":"A. Ibrahim","doi":"10.21608/EJGC.2014.9922","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9922","url":null,"abstract":"The adrenergic receptor β3 (ADRβ3) is the major regulator of lipolysis and homeostasis and predominantly expressed in brown and white adipose tissues (Cannon and Nedergaard, 2004). The ADRβ3 gene which encodes for this receptor was studied as a candidate gene associated with growth traits, body dimensional measurements and conformation indices of Barki sheep. Single strand conformational polymorphism (SSCP) was used to identify the variation in the intron region of ADRβ3 gene for 136 male and female Barki lambs. Eighteen SSCP genotypes representing six alleles (A, B, C, D, E and H) were detected. The frequencies of these alleles were 0.331, 0.184, 0.283, 0.044, 0.073 and 0.084, respectively. The results indicated that ADRβ3 genotype significantly (P ˂ 0.05) affected marketing weight, thigh circumference, body mass index and skeletal muscle index; and also significantly (P ˂ 0.01) affected post-weaning daily gain. Least square means analysis showed that animals with genotypes AA, AC and CC had higher post-weaning daily gain, marketing weight, thigh circumference, body mass index and skeletal muscle index than animals with genotypes CD, CE and CH. In view of the obtained results, molecular marker selection using ADRβ3 gene is warranted to increase post-weaning growth rate, whole-body mass and skeletal muscle mass in Barki sheep and will be of considerable economic value to sheep producer.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"287-299"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The calpain 3 (CAPN3) is a major intracellular protease encoded by CAPN3 gene and mainly expressed in skeletal muscle. This protease is necessary for normal muscle function, as mutations in CAPN3 result in increasing muscle protein degradation. Polymerase chain reaction-single strand conformational polymor-phism (PCR-SSCP) was used to identify allelic and genotypic polymorphisms in exon 10 of CAPN3 gene for 24 Egyptian Barki lambs. Associations between the identified polymorphisms and growth and carcass traits were determined. Three al-leles A, B and C were identified with fre-quency 0.79, 0.14 and 0.07, respectively. Only three genotypes were identified AA, AB and AC with frequency 0.58, 0.29 and 0.13, respectively. CAPN3 genotypes sig-nificantly affected post-weaning daily gain (P < 0.005), marketing weight (P < 0.05) and lean-meat% (P<0.05). Animals with AB genotype had higher means for post-weaning daily gain; marketing weight and lean-meat%. In contrast, ani-mals with AC genotype had the lower means for these traits. Our findings sug-gest that the polymorphisms in CAPN3 may play a role affecting growth rate and lean meat% of carcass in Egyptian Barki sheep.
{"title":"VARIATION IN EXON 10 OF THE OVINE CALPAIN3 GENE AND ITS ASSOCIATION WITH GROWTH AND CARCASS TRAITS IN EGYPTIAN BARKI LAMBS","authors":"M. Shehata, I. Ismail, A. Ibrahim","doi":"10.21608/EJGC.2014.9918","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9918","url":null,"abstract":"The calpain 3 (CAPN3) is a major intracellular protease encoded by CAPN3 gene and mainly expressed in skeletal muscle. This protease is necessary for normal muscle function, as mutations in CAPN3 result in increasing muscle protein degradation. Polymerase chain reaction-single strand conformational polymor-phism (PCR-SSCP) was used to identify allelic and genotypic polymorphisms in exon 10 of CAPN3 gene for 24 Egyptian Barki lambs. Associations between the identified polymorphisms and growth and carcass traits were determined. Three al-leles A, B and C were identified with fre-quency 0.79, 0.14 and 0.07, respectively. Only three genotypes were identified AA, AB and AC with frequency 0.58, 0.29 and 0.13, respectively. CAPN3 genotypes sig-nificantly affected post-weaning daily gain (P < 0.005), marketing weight (P < 0.05) and lean-meat% (P<0.05). Animals with AB genotype had higher means for post-weaning daily gain; marketing weight and lean-meat%. In contrast, ani-mals with AC genotype had the lower means for these traits. Our findings sug-gest that the polymorphisms in CAPN3 may play a role affecting growth rate and lean meat% of carcass in Egyptian Barki sheep.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"231-240"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leaf stripe in barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. In the present work, a QTL map for F2 barley and 7 LSLs) distributed on 8 linkage groups and spanning 2099.4 cM of the barley genome. The size of linkage groups varied from 117.8 cM for LG2 (2H) to 482.9 cM for LG6 (6H) with an average length of 262.4 cM. Based on the used anchor SSR markers, only four linkage groups were assigned to chromosomes, where LG2, LG5, LG6 and LG7 were assigned to chromosomes 2H, 5H, 6H and 7H, respectively. Single point analysis was used to identify genomic regions controlling the leaf stripe severity and plant height traits. A total of 40 QTL were identified for the disease severity (DS) and plant height (PH) traits (20 QTL for disease severity and 20 QTL for plant height). This work represents the first genetic linkage map for barley population derived from an intraspecific cross between At20 and At29 showing chromosomal regions associated with disease severity and plant height traits in barley.
大麦叶条病是一种由稻瘟病菌(Pyrenophora graminea)引起的重要的种子传播病害，在北欧和地中海大麦区也有发生。在本研究中，构建了一个F2和7个LSLs的QTL图谱，分布在8个连锁群上，覆盖了大麦基因组的2099.4 cM。LG2 (2H)的连锁群长度为117.8 cM, LG6 (6H)的连锁群长度为482.9 cM，平均长度为262.4 cM。根据所使用的锚点SSR标记，仅将4个连锁群分配到染色体上，其中LG2、LG5、LG6和LG7分别分配到2H、5H、6H和7H染色体上。采用单点分析鉴定控制叶条严重程度和株高性状的基因组区域。共鉴定出40个与病重(DS)和株高(PH)性状相关的QTL(病重20个，株高20个)。这项工作代表了大麦群体的第一个遗传连锁图谱，该图谱来源于At20和At29之间的种内杂交，显示了大麦中与疾病严重程度和株高性状相关的染色体区域。
{"title":"MAPPING OF QTLs CONTROLLING RESISTANCE TO LEAF STRIPE (Pyrenophora graminea) IN EGYPTIAN BARLEY","authors":"S. Adawy, R. Ismail, M. Sakr","doi":"10.21608/EJGC.2014.9917","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9917","url":null,"abstract":"Leaf stripe in barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. In the present work, a QTL map for F2 barley and 7 LSLs) distributed on 8 linkage groups and spanning 2099.4 cM of the barley genome. The size of linkage groups varied from 117.8 cM for LG2 (2H) to 482.9 cM for LG6 (6H) with an average length of 262.4 cM. Based on the used anchor SSR markers, only four linkage groups were assigned to chromosomes, where LG2, LG5, LG6 and LG7 were assigned to chromosomes 2H, 5H, 6H and 7H, respectively. Single point analysis was used to identify genomic regions controlling the leaf stripe severity and plant height traits. A total of 40 QTL were identified for the disease severity (DS) and plant height (PH) traits (20 QTL for disease severity and 20 QTL for plant height). This work represents the first genetic linkage map for barley population derived from an intraspecific cross between At20 and At29 showing chromosomal regions associated with disease severity and plant height traits in barley.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"207-230"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seedlings of Six maize inbred lines CML 511, CML 448, CML 444, CML 395, CML 254 and CML 216 were used to analyze salt stress of (150 mM NaCl). The analyses of RAPD results showed 84.6% of polymorphic fragments. Accessions of CML 511 and CML 448 were included in one main cluster with genetic similarity of 0.65. The genetic similarity between CML 444 and CML 395 was 0.72, the other accessions of CML 254 and CML 216 had genetic similarities of 0.67 and 0.62, respectively. Leaf proteins were studied by one-dimensional SDS-PAGE. Under non stress conditions, the minor band of 45 kDa was not expressed in varieties of CML 254 and CML 216 while band of 23 kDa were expressed in all accessions except of CML 216 accession. Under stress conditions high molecular weight bands were disappeared and the major protein bands were located at the medium molecular weight range. Protein spectrofluorescence of leaf protein samples show main emission peak at about 320 nm in both conditions of salt stress and control. However, the emission intensities showed some variations among different maize lines in alternative conditions of control and salt stress. This might be referred to change in protein interactions. Inbred lines of CML 511 and CML 448 exhibit closely related fluorescence pattern, this is in context of their genetic similarities as has deduced by RAPD results.
{"title":"MONITORING OF SALT TOLERANCE RESPONSES OF DIFFER-ENT MAIZE INBRED LINES USING PROTEIN PROFILE AND PROTEIN SPECTROFLUORESCENCE","authors":"N. Abbas, A. Fayed","doi":"10.21608/EJGC.2014.9919","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9919","url":null,"abstract":"Seedlings of Six maize inbred lines CML 511, CML 448, CML 444, CML 395, CML 254 and CML 216 were used to analyze salt stress of (150 mM NaCl). The analyses of RAPD results showed 84.6% of polymorphic fragments. Accessions of CML 511 and CML 448 were included in one main cluster with genetic similarity of 0.65. The genetic similarity between CML 444 and CML 395 was 0.72, the other accessions of CML 254 and CML 216 had genetic similarities of 0.67 and 0.62, respectively. Leaf proteins were studied by one-dimensional SDS-PAGE. Under non stress conditions, the minor band of 45 kDa was not expressed in varieties of CML 254 and CML 216 while band of 23 kDa were expressed in all accessions except of CML 216 accession. Under stress conditions high molecular weight bands were disappeared and the major protein bands were located at the medium molecular weight range. Protein spectrofluorescence of leaf protein samples show main emission peak at about 320 nm in both conditions of salt stress and control. However, the emission intensities showed some variations among different maize lines in alternative conditions of control and salt stress. This might be referred to change in protein interactions. Inbred lines of CML 511 and CML 448 exhibit closely related fluorescence pattern, this is in context of their genetic similarities as has deduced by RAPD results.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"241-255"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68478764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study the polymorphisms in exon 2 of ovine Forkhead Box Class O3 (FOXO3) gene were detected in 96 Barki ewes by the polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. The association of FOXO3 gene polymorphisms with lifespan traits was tested using general linear mixed effect models. Three alleles (A, B and C) were detected with frequencies of 0.57, 0.28 and 0.15, respectively. Also five genotypes (AA, AB, AC, BB and CC) were identified with frequencies of 0.26, 0.44, 0.18, 0.06 and 0.06, respectively. Genotypes of the FOXO3 gene were shown to be associated with age, twining rate, total number of lambs born per ewe (TNLB) and total weight of lambs born per ewe (TWLB). The presence of allele C was associated with longer age and increased TNLB, whereas the presence of allele B was associated with shorter age, increased twining rate and decreased rearing ability and total number of lams weaned per ewe (TNLW). The effect of the number of allele copies present was tested and it was found that the presence of two copies of allele C was associated with longer age and increased TNLB and the presence of two copies of allele B was associated with shorter age, increased twining rate and decreased rearing ability and TNLW. These results suggest that variation in ovine FOXO3 gene is associated with lifespan traits of Barki ewes.
{"title":"VARIATION IN THE FORKHEAD BOX CLASS O3 (FOXO3) GENE AND ITS ASSOCIATION WITH LIFESPAN TRAITS IN BARKI EWES","authors":"A. Ibrahim, S. Alsheikh","doi":"10.21608/EJGC.2014.9925","DOIUrl":"https://doi.org/10.21608/EJGC.2014.9925","url":null,"abstract":"In this study the polymorphisms in exon 2 of ovine Forkhead Box Class O3 (FOXO3) gene were detected in 96 Barki ewes by the polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. The association of FOXO3 gene polymorphisms with lifespan traits was tested using general linear mixed effect models. Three alleles (A, B and C) were detected with frequencies of 0.57, 0.28 and 0.15, respectively. Also five genotypes (AA, AB, AC, BB and CC) were identified with frequencies of 0.26, 0.44, 0.18, 0.06 and 0.06, respectively. Genotypes of the FOXO3 gene were shown to be associated with age, twining rate, total number of lambs born per ewe (TNLB) and total weight of lambs born per ewe (TWLB). The presence of allele C was associated with longer age and increased TNLB, whereas the presence of allele B was associated with shorter age, increased twining rate and decreased rearing ability and total number of lams weaned per ewe (TNLW). The effect of the number of allele copies present was tested and it was found that the presence of two copies of allele C was associated with longer age and increased TNLB and the presence of two copies of allele B was associated with shorter age, increased twining rate and decreased rearing ability and TNLW. These results suggest that variation in ovine FOXO3 gene is associated with lifespan traits of Barki ewes.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"339-352"},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68479422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：