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Fusing cross-source spectral data and component-driven analysis for maize grain quality detection 融合跨源光谱数据及成分驱动分析用于玉米籽粒品质检测
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.foodcont.2026.112004
Qixing Tang , Dong Wei , Longbao Liu , Juan Liao , Leizi Jiao , Yachao Liu , Ke Wang , Runyu Wang , Daming Dong
This study addresses the limitations of traditional chemical methods and the poor generalizability of single near-infrared spectroscopy models by proposing a component-driven approach for universal maize quality assessment. The core of our methodology is a novel workflow in which predicted component contents are used as features for quality classification. First, spectral discrepancies between public and laboratory datasets were mitigated through waveband alignment and selection (1180–1460 nm). Subsequently, after optimizing pre-processing algorithms, we employed models trained on public data to predict the contents of key components (e.g., moisture, fat) in laboratory samples. These predicted component values, rather than the raw spectral data, were then used as inputs to machine learning models for intelligent quality classification. Experimental results showed that the Random Forest (RF) model, utilizing the predicted components and Savitzky-Golay + Baseline Correction (SG + BC) pre-processing, achieved optimal performance with a test set accuracy of 91.88 %. This research provides a novel strategy for non-destructive quality evaluation by fusing cross-source spectral data and multi-component synergy, which significantly enhances the model's generalization capability and practical applicability.
本研究提出了一种组分驱动的通用玉米质量评价方法,解决了传统化学方法的局限性和单一近红外光谱模型的通用性差的问题。该方法的核心是一种新颖的工作流程,其中预测的组件内容被用作质量分类的特征。首先,通过波段对准和选择(1180-1460 nm)减轻了公共和实验室数据集之间的光谱差异。随后,在优化预处理算法后,我们使用公共数据训练的模型来预测实验室样品中关键成分(如水分、脂肪)的含量。这些预测的成分值,而不是原始的光谱数据,然后被用作机器学习模型的输入,用于智能质量分类。实验结果表明,利用预测分量和Savitzky-Golay + Baseline Correction (SG + BC)预处理的随机森林(Random Forest, RF)模型达到了最佳性能,测试集准确率为91.88%。本研究提出了一种融合跨源光谱数据和多分量协同的无损质量评价新策略,显著提高了模型的泛化能力和实用性。
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引用次数: 0
Genomic and one health insights into enterotoxigenic Clostridium perfringens in retail shellfish: Evidence of environmental and zoonotic circulation 零售贝类中产肠毒素产气荚膜梭菌的基因组学和健康见解:环境和人畜共患循环的证据
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-20 DOI: 10.1016/j.foodcont.2026.112003
Yeona Kim , Hyeonwoo Cho , Kun Taek Park
Clostridium perfringens is a widespread pathogen responsible for gastrointestinal diseases in both humans and animals, including foodborne illness. Among its toxinotypes, enterotoxigenic strains harboring the cpe gene are of particular concern due to their role in human infections and environmental persistence. Despite increasing seafood-related outbreaks, data on the prevalence and genomic characteristics of C. perfringens in retail shellfish are limited. Here, we investigated the occurrence, antimicrobial resistance (AMR) profiles, and genetic characteristics of C. perfringens isolated from manila clams and abalones obtained from Korean retail markets. The overall prevalence was 71.0 % (142/200) in manila clams and 64.5 % (129/200) in abalones. Toxinotyping identified type A as predominant (85.6 %), and 12.5 % of isolates carried the cpe gene. The highest level of AMR was observed against tetracycline (43.9 %). Whole-genome sequencing of 30 cpe-positive isolates revealed the presence of 15 virulence genes and 2 AMR determinants, with most strains harboring plasmid-borne IS1151-cpe elements. Phylogenomic analysis demonstrated close genetic relatedness between seafood-derived and human clinical strains, suggesting possible bidirectional transmission between aquatic environments and humans. These findings highlight the role of shellfish as sentinel species for tracking human-derived fecal pollution and emphasize the need for integrated One Health surveillance strategies to monitor the dissemination of AMR and toxigenic C. perfringens across aquatic, environmental, and foodborne pathways.
产气荚膜梭菌是一种广泛存在的病原体,可导致人类和动物的胃肠道疾病,包括食源性疾病。在其毒素类型中,肠产毒素菌株由于其在人类感染和环境持久性中的作用而受到特别关注。尽管与海产品有关的疫情不断增加,但关于零售贝类中产气荚膜荚膜梭菌的流行率和基因组特征的数据有限。在这里,我们研究了从韩国零售市场上获得的马尼拉蛤和鲍鱼中分离的产气荚膜杆菌的发生、耐药性(AMR)谱和遗传特征。马尼拉蛤总患病率为71.0%(142/200),鲍鱼总患病率为64.5%(129/200)。毒素分型鉴定为A型为主(85.6%),12.5%的分离株携带cpe基因。其中四环素组AMR最高(43.9%)。30株cpe阳性菌株的全基因组测序显示存在15个毒力基因和2个AMR决定因子,其中大多数菌株含有质粒携带的IS1151-cpe元素。系统基因组学分析表明,海产菌株与人类临床菌株之间存在密切的遗传关系,表明水生环境与人类之间可能存在双向传播。这些发现强调了贝类作为追踪人类来源的粪便污染的前点物种的作用,并强调了统一健康监测战略的必要性,以监测抗菌素耐药性和产毒素产气荚膜梭菌在水生、环境和食源性途径中的传播。
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引用次数: 0
Efficient removal of aflatoxin B1 from soybean kernels by a coupled ultrasound–ozone process: A mechanistic study 超声-臭氧耦合去除大豆籽粒中黄曲霉毒素B1的机理研究
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.foodcont.2026.111988
Xiangyan Zeng, Shuke Liu, Haitao Yu, Laxiang Yang
Aflatoxin B1 (AFB1) contamination in soybean kernels poses a persistent food safety challenge. This study evaluated the coupled ultrasound–ozone (US/O3) process for AFB1 removal from soybean kernels. After 10 min of treatment, the US/O3 process removed 96.2 % of AFB1, far exceeding ozone alone (54.5 %), ultrasound alone (36.9 %), and water washing (18.1 %). Mass balance analysis showed that only 10.4 % of the initial AFB1 remained in the kernels and soaking water after US/O3 treatment, indicating that the process mainly destroyed AFB1 rather than merely transferring it into the liquid phase. A positive, time-dependent synergy factor quantified the cooperative effect between ultrasound and ozone. Electron spin resonance (ESR) spin-trapping combined with radical scavenging experiments identified hydroxyl radicals (HO) as the dominant oxidants. Identified transformation products suggested an initial attack on the terminal furan ring, followed by extensive hydroxylation, ring opening, dealkylation, and fragmentation to low-molecular-weight phenolic products. ECOSAR predictions showed higher LC50 values for most products than for AFB1, indicating reduced acute toxicity. Fourier transform infrared (FTIR) spectra, color parameters, and lipid oxidation indices indicated negligible changes in soybean composition and quality. Therefore, the US/O3 process offers an efficient, quality-preserving strategy for mitigating AFB1 contamination in soybean kernels.
黄曲霉毒素B1 (AFB1)污染的大豆仁是一个持续的食品安全挑战。研究了超声-臭氧(US/O3)耦合去除大豆籽粒中AFB1的工艺。经过10 min的处理,US/O3工艺对AFB1的去除率为96.2%,远远超过单纯臭氧法(54.5%)、超声法(36.9%)和水洗法(18.1%)。质量平衡分析表明,经过US/O3处理后,只有10.4%的初始AFB1留在了稻壳和浸泡水中,这表明该过程主要是破坏了AFB1,而不仅仅是将其转移到液相。一个积极的、时间相关的协同因子量化了超声和臭氧之间的协同效应。电子自旋共振(ESR)自旋捕获结合自由基清除实验发现羟基自由基(HO•)是主要的氧化剂。鉴定的转化产物表明,最初的攻击是在末端的呋喃环上,随后是广泛的羟基化,开环,脱烷基,裂解成低分子量的酚类产物。ECOSAR预测显示,大多数产品的LC50值高于AFB1,表明急性毒性降低。傅里叶变换红外光谱(FTIR)、颜色参数和脂质氧化指标表明,大豆成分和品质的变化可以忽略不计。因此,US/O3工艺为减轻大豆籽粒中AFB1的污染提供了一种有效的、保持质量的策略。
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引用次数: 0
Mechanism of texture formation in dried fruits and vegetables: from macroscopic to the molecular level 干果和蔬菜的质地形成机制:从宏观到分子水平
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.foodcont.2026.111998
Rongtian An , Linlin Li , Lijuan Wang , Wenchao Liu , Weiwei Cao , Junliang Chen , Guangyue Ren , Min Zhang , Chung Lim Law , Xu Duan
With the growing consumer demand for enhanced mouthfeel of dried fruits and vegetables, texture has emerged as a key indicator of product quality. However, most existing studies regard texture merely as a final attribute, lacking a systematic exploration of the underlying formation mechanisms. This knowledge gap poses a significant barrier to product innovation and process optimization in the dried fruit and vegetable industry. This review presents a comprehensive overview of current evaluation methods and research progress related to the textural properties of dried fruits and vegetables. Emphasis is placed on the multiscale structural and compositional transformations that occur during drying, driven by coupled heat and mass transfer processes. A multiscale framework is proposed to elucidate the underlying mechanisms across four hierarchical levels: (1) physical state changes and structural shrinkage at the macroscopic level, (2) pore structure evolution at the mesoscopic level, (3) morphological responses at the cellular level, and (4) compositional reorganization at the molecular level. Moreover, this review underscores the existing research gaps at the cellular and molecular levels and outlines potential future directions. For instance, the mechanisms underlying intercellular adhesion loss, domain-specific degradation of polysaccharides (e.g., homogalacturonan, rhamnogalacturonans I and II), and the regulation of endogenous enzymes during drying remain poorly understood. These unresolved issues constrain our ability to design drying protocols that can precisely modulate textural properties.
随着消费者对果干和蔬菜口感要求的不断提高,果干和蔬菜的质地已成为衡量产品质量的关键指标。然而,现有的研究大多将纹理视为最终属性,缺乏对其形成机制的系统探索。这种知识差距对干果和蔬菜行业的产品创新和工艺优化构成了重大障碍。本文综述了国内外干果和蔬菜的质地特性评价方法及研究进展。重点放在干燥过程中发生的多尺度结构和成分转变,由耦合的传热和传质过程驱动。提出了一个多尺度的框架来阐明四个层次的潜在机制:(1)宏观水平的物理状态变化和结构收缩,(2)介观水平的孔隙结构演化,(3)细胞水平的形态响应,以及(4)分子水平的成分重组。此外,本文还强调了目前在细胞和分子水平上的研究空白,并概述了潜在的未来发展方向。例如,细胞间粘附丧失的机制,多糖的特定区域降解(例如,高半乳葡聚糖,鼠李糖半乳葡聚糖I和II),以及干燥过程中内源性酶的调节仍然知之甚少。这些未解决的问题限制了我们设计干燥协议的能力,可以精确地调节纹理特性。
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引用次数: 0
Temperature-dependent effects of preheating on the freeze-thaw stability of non-fermented dough: Multi-scale structural changes in gluten and starch 预热对非发酵面团冻融稳定性的温度依赖性:面筋和淀粉的多尺度结构变化
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.foodcont.2026.111993
Yingman Xie, Haimeng Zhu, Zihaocheng Wang, Qinxiu Chen, Xiangwei Chen, Hongfei Fu, Yequn Wang, Zhenna Zhang, Yunyang Wang
Repeated freeze-thaw (FT) cycles deteriorate the quality of frozen dough by disrupting the structural integrity of gluten and starch macromolecules. This study systematically investigated the effects of preheating temperatures (45 °C–65 °C) and FT cycles (0–8) on the quality and macromolecular characteristics of non-fermented dough to identify the optimal temperature window and elucidate the underlying mechanisms. The results demonstrated that while all doughs exhibited a degradation in textural and rheological properties with increasing FT cycles, a distinct temperature-dependent effect was evident. Preheating at 50 °C–55 °C proved more effective in mitigating dough degradation, outperforming treatment at 45 °C, whereas treatments at 60 °C and 65 °C resulted in significant quality loss. The enhancement mechanism was primarily attributed to the optimal preheating (50 °C–55 °C), which reinforced the gluten network by promoting disulfide bond formation, evidenced at the initial state (FT-0) by increased glutenin macropolymer (GMP) content and decreased free sulfhydryl (-SH) levels compared to the control. This reinforced matrix restricted water mobility and physically hindered starch recrystallization during FT cycles. This research elucidates the temperature-dependent mechanism by which moderate thermal pretreatment enhances the FT stability of non-fermented dough, establishing a clear structure-function relationship for gluten and starch macromolecules. Moreover, it provides a scientific basis for innovative strategies aimed at improving frozen dough quality and facilitating the development of new frozen flour products.
反复的冻融循环会破坏面筋和淀粉大分子的结构完整性,从而降低冷冻面团的质量。本研究系统研究了预热温度(45°C - 65°C)和FT循环(0-8)对非发酵面团质量和大分子特性的影响,以确定最佳温度窗口并阐明其潜在机制。结果表明,虽然随着FT循环次数的增加,所有面团的质地和流变性能都有所下降,但明显存在明显的温度依赖效应。50°C - 55°C的预热被证明在缓解面团降解方面更有效,优于45°C的处理,而60°C和65°C的处理会导致明显的质量损失。增强机制主要归因于最佳预热(50°C - 55°C),通过促进二硫键的形成来增强面筋网络,在初始状态(FT-0)与对照相比,增加了谷蛋白大聚合物(GMP)含量,降低了游离巯基(-SH)水平。这种增强的基质限制了水的流动性,并在物理上阻碍了淀粉在FT循环中的再结晶。本研究阐明了适度热预处理提高非发酵面团FT稳定性的温度依赖机制,明确了面筋和淀粉大分子的结构-功能关系。为提高冷冻面团质量和开发新型冷冻面粉产品的创新策略提供了科学依据。
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引用次数: 0
Advances in active packaging for enhanced food safety: Insights into functional additives, response strategies and applications 提高食品安全的活性包装的进展:对功能性添加剂,响应策略和应用的见解
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.foodcont.2026.111989
Huajuan Wang , Yi Liu , Yang Yi , Ting Min , Hongxun Wang , Liang Ke , Wenhao Hu , Changqing Hang , Zhiyong Song , Min Zhou
Given the substantial food waste occurring throughout the supply chain, the critical role of food packaging is increasingly evident. Nevertheless, contemporary food packaging largely depends on physical barriers, which fail to provide continuous protection or adapt to changes in the packaging environment. Active packaging has emerged as a promising solution, offering enduring protection to food by controlled, on-demand release of functional agents. This protective approach demonstrates considerable promise not only in extending the shelf life of products but also in elevating food safety standards and alleviating losses resulting from spoilage. Active packaging technology incorporates key functionalities, notably antimicrobial, antioxidant, and food freshness monitoring capabilities. Furthermore, extensive research into how various packaging materials respond to environmental stimuli is crucial for developing precise and effective food preservation strategies. Hence, this paper provides an overview of recent developments in active packaging, focusing on the types of functional additives typically incorporated, various release mechanisms based on specific stimuli, endogenous (such as pH, humidity, etc.), exogenous (such as light, temperature), or multiple stimuli (humidity and pH, pH and temperature, etc.). It further discusses the challenges confronting active packaging systems, such as material stability, reliability, and safety issues, while also projecting future trends. The aim is to offer valuable insights for developing more efficient, intelligent, and multifunctional active food packaging, thereby effectively extending the shelf life of food and reducing food waste.
鉴于整个供应链中发生的大量食物浪费,食品包装的关键作用越来越明显。然而,当代食品包装在很大程度上依赖于物理屏障,不能提供持续的保护,也不能适应包装环境的变化。活性包装已成为一个有前途的解决方案,提供持久的保护,通过控制,按需释放功能剂的食品。这种保护方法不仅在延长产品的保质期,而且在提高食品安全标准和减轻腐败造成的损失方面显示出相当大的希望。活性包装技术包含关键功能,特别是抗菌、抗氧化和食品新鲜度监测功能。此外,广泛研究各种包装材料对环境刺激的反应对于制定精确有效的食品保存策略至关重要。因此,本文概述了活性包装的最新发展,重点介绍了通常包含的功能添加剂的类型,基于特定刺激的各种释放机制,内源性(如pH值,湿度等),外源性(如光,温度)或多种刺激(湿度和pH值,pH值和温度等)。它进一步讨论了活性包装系统面临的挑战,如材料稳定性、可靠性和安全性问题,同时也预测了未来的趋势。目的是为开发更高效、智能和多功能的活性食品包装提供有价值的见解,从而有效地延长食品的保质期,减少食品浪费。
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引用次数: 0
Insights into quality evaluation, factors, deterioration mechanisms, and improving strategies of livestock and its prepared dishes: An updated overview 洞察质量评价,因素,变质机制,和改进策略的牲畜和它准备的菜肴:一个最新的概述
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-19 DOI: 10.1016/j.foodcont.2026.111992
Jiaqi Tan , Xin Du , Haijing Li , Ying Li , Quanyu Zhang , Xiufang Xia , Yujuan Xu
Livestock and its prepared dishes (LPDs) have gradually become a new highlight in the meat consumption market with the rapid development of new catering methods in recent years. However, quality deterioration is inevitable during processing, storage, transportation, and packaging. Nowadays, consumers demand healthier and higher quality meat dishes compared to previous demands. Exploring the factors affecting the quality of LPDs, analyzing the mechanisms of quality deterioration, and finding strategies to improve their quality are crucial for growing market consumption. This paper's main objectives are as follows: 1) review the quality evaluation of livestock (color, tenderness, flavor, and water holding capacity); 2) discuss the reasons and mechanisms for the quality formation of LPDs; 3) comprehensively summarize the physical, chemical, and biological methods for improving their quality; 4) systematically review the challenges and prospects currently faced by LPDs industry. The findings can provide new ideas for the standardization, safety, nutrition, and intelligence of LPDs, as well as the reference for innovation and sustainable development of the prepared dishes industry.
近年来,随着新型餐饮方式的快速发展,畜禽及其配菜逐渐成为肉类消费市场的新亮点。然而,在加工、储存、运输和包装过程中,质量恶化是不可避免的。如今,消费者对肉类菜肴的需求比以前更健康、品质更高。探索影响石油品质量的因素,分析石油品质量恶化的机理,提出提高石油品质量的对策,对扩大石油品市场消费具有重要意义。本文的主要目的如下:1)综述了畜禽的质量评价(色、嫩、味、保水性);2)探讨lpd质量形成的原因和机制;3)综合总结提高其质量的物理、化学和生物方法;4)系统回顾了目前lng行业面临的挑战和前景。研究结果可为食品配料的标准化、安全性、营养性和智能化提供新的思路,为食品配料行业的创新和可持续发展提供参考。
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引用次数: 0
Correlation study between sesame seeds roasting conditions and the physicochemical characteristics, hazardous substances levels, and volatile substances profile of hot-water-substitution extracted flavor sesame (Sesamum indicum L.) oil 芝麻焙烧条件与热水替代风味芝麻油理化特性、有害物质含量及挥发性物质分布的相关性研究
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-16 DOI: 10.1016/j.foodcont.2026.111976
Li Zhou , Qingyang Zhang , Jin Zhang , Jinglun Zhou , Xinya Huang , Zhengting Zhu , Siya Zhao , Meiyu Zheng , Yong Yang , Shu Wang , Bin Li , Sihong Zhang , Dongping He , Zhigang Hu , Fenfen Lei
Sesame oil, esteemed for its robust aroma and high content of unsaturated fatty acids and micronutrients, depends predominantly on seed roasting to develop its characteristic flavor. However, this high-temperature process is associated with the risk of generating hazardous compounds, including benzo [a]pyrene (BaP), 3-monochloropropane-1,2-diol esters (3-MCPDE), and glycidyl esters (GEs), which have raised increasing regulatory concerns worldwide. In this study, an integrated approach was employed, encompassing sample morphology, physicochemical properties, levels of hazardous substances, and flavor profiles, to systematically investigate the influence of roasting conditions (180–260 °C for 10–40 min) on the quality, safety, and flavor of hot-water-substitution-extracted sesame oil. Results revealed that roasting induced significant microstructural degradation in the sesame seeds, darkened the oil color, and lowered the acid value, while the peroxide value was increased. Importantly, the concentrations of BaP, 3-MCPDE, and GEs exhibited a pronounced rise when roasting temperature exceeded 220 °C, establishing this threshold as a critical control point for process safety. The relative contents of polyunsaturated fatty acids, such as linoleic and linolenic acid, were slightly reduced under intensified roasting. In parallel, a concomitant increase in key volatile compounds was confirmed by electronic-nose analysis, with short-chain alkanes, aldehydes, and alcohols identified as the principal volatile substances. It was concluded that roasting conditions must be meticulously optimized to balance flavor development against the formation of processing contaminants and the degradation of nutritional components. These findings provide a crucial theoretical foundation for refining industrial sesame-oil production and for establishing science-based criteria in market quality supervision and regulatory standards.
芝麻油香气浓郁,富含不饱和脂肪酸和微量营养素,其特有风味主要依靠种子烘烤。然而,这种高温过程与产生有害化合物的风险有关,包括苯并[a]芘(BaP), 3-一氯丙烷-1,2-二醇酯(3-MCPDE)和甘油三酯(GEs),这些化合物在世界范围内引起了越来越多的监管关注。在本研究中,采用综合的方法,包括样品的形态,物理化学性质,有害物质水平和风味特征,系统地研究了烘焙条件(180-260°C, 10-40分钟)对热水替代萃取芝麻油的质量,安全性和风味的影响。结果表明,焙烧引起芝麻微观结构的明显降解,使芝麻油颜色变暗,酸值降低,过氧化值升高。重要的是,当焙烧温度超过220℃时,BaP、3-MCPDE和GEs的浓度明显升高,这表明该阈值是工艺安全的关键控制点。强化焙烧后,亚油酸和亚麻酸等多不饱和脂肪酸的相对含量略有降低。与此同时,电子鼻分析证实了主要挥发性化合物的增加,其中短链烷烃、醛和醇被确定为主要挥发性物质。因此,必须精心优化烘焙条件,以平衡风味的发展,防止加工污染物的形成和营养成分的降解。研究结果为麻油工业生产的精细化和市场质量监督标准的科学制定提供了重要的理论依据。
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引用次数: 0
Biofilm-forming capability and susceptibility to disinfectants among bacterial isolates from seafood processing in Québec 衢州海产品加工分离细菌的生物膜形成能力及对消毒剂的敏感性
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-14 DOI: 10.1016/j.foodcont.2026.111974
Lydia Ait Said , Jean-Philippe Langlois , Laurie Sanschagrin , Éric Jubinville , Valérie Goulet-Beaulieu , Julie Jean
Fish and seafood processing faces persistent microbial contamination issues, due largely to biofilm formation on equipment and product contact surfaces, despite sanitation procedures. In this study, the bacterial diversity found on production lines processing shrimp, smoked salmon (artisanal and industrial-scale), snow crab and lobster was evaluated in terms of biofilm-forming ability and resistance to two conventional and two alternative disinfection treatments.
From a total of 105 surfaces sampled after sanitation, 472 bacterial isolates were obtained, representing 53 genera, primarily Pseudomonas, Aeromonas, and Acinetobacter. While most isolates produced weak biofilms, moderate to strong biofilm producers were mainly Pseudomonas (43 %), Aeromonas (22 %), and Acinetobacter (10 %).
Nine representative isolates were tested against peracetic acid. Peracetic acid (100 ppm) reduced viable counts by > 3 log cfu/mL in four isolates, although two showed marked resistance (<1 log reduction). Sodium hypochlorite (70 ppm) was effective against five isolates, while one was resistant. Reuterin (258 mM) proved ineffective against all isolates, whereas pulsed light (11.52 J/cm2) achieved reduction greater than 3 log for most strains.
These results highlight the persistence of biofilm-forming bacteria after sanitation and demonstrate that resistance varies across genera and treatments. In particular, Pseudomonas and Acinetobacter emerge as key genera capable of withstanding conventional disinfectants, emphasizing the need to optimize both chemical and non-chemical disinfection strategies to improve hygiene in seafood processing.
尽管有卫生程序,但鱼和海鲜加工面临着持续的微生物污染问题,这主要是由于设备和产品接触表面形成的生物膜。在本研究中,对虾、熏鲑鱼(手工和工业规模)、雪蟹和龙虾的生产线上发现的细菌多样性进行了生物膜形成能力和对两种常规和两种替代消毒处理的抗性评估。从卫生后取样的105个表面中,获得了472个细菌分离株,代表53属,主要是假单胞菌、气单胞菌和不动杆菌。虽然大多数菌株产生弱生物膜,但中等至强生物膜主要是假单胞菌(43%),气单胞菌(22%)和不动杆菌(10%)。9株有代表性的分离株对过氧乙酸进行了抑菌试验。过氧乙酸(100 ppm)使四个分离株的活菌计数降低了3 log cfu/mL,尽管其中两个表现出明显的抗性(降低了1 log)。次氯酸钠(70 ppm)对5株菌株有效,1株菌株耐药。Reuterin (258 mM)对所有菌株无效,而脉冲光(11.52 J/cm2)对大多数菌株的抑制效果大于3 log。这些结果强调了卫生后形成生物膜的细菌的持久性,并表明抗性在不同的属和处理中有所不同。特别是,假单胞菌和不动杆菌是能够承受传统消毒剂的关键属,强调需要优化化学和非化学消毒策略,以改善海鲜加工中的卫生。
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引用次数: 0
Dual H–O isotope tracing of oxidized triacylglycerols in triolein (180°C): mechanisms and control-oriented monitoring indicators 氧化三甘油酯在三油酸(180°C)中的双H-O同位素示踪:机制和面向控制的监测指标
IF 6.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-01-14 DOI: 10.1016/j.foodcont.2026.111972
Yiting Duan , Weidong Su , Xinru Deng , Chongyang Liu , Qi Li , Yuan Gao , Xiuzhu Yu
Oxidized triacylglycerols (OxTGs) formed during frying degrade oil quality and raise safety concerns. To resolve oxygen provenance under frying-like conditions, a dual hydrogen–oxygen stable-isotope tracing design (D2O, H218O, 18O2) was applied to triolein heated at 180 °C for 0–4 h under 3 atm: natural air (NA) + H2O, synthetic air (SA)–16O2 + H218O, and SA–18O2 + D2O. Diagnostic fragment ions revealed five triacylglycerols and eight OxTGs dominated by hydroperoxide (–OOH) and hydroxyl (–OH) functionalities, while isotopologue ratios (M+1–M+4)/M quantified label incorporation. Pronounced enrichment occurred only under SA–18O2 + D2O, particularly for hydroxylated OxTGs; ratios remained comparatively stable in NA + H2O and SA–16O2 + H218O, consistent with water acting primarily as a kinetic modulator of hydroperoxide turnover and interfacial processes rather than as a direct oxygen donor to OxTGs.The labeling pattern demonstrates that oxygen incorporated into OxTGs derives primarily from molecular oxygen (O2). Mechanistically grounded process controls that limit oxygen availability—such as vacuum or otherwise deaerated frying—are therefore expected to suppress OxTG formation. For decision-ready surveillance and oil-turnover management, an integrated indicator panel is recommended, combining total polar compounds (TPC), sentinel glycerol-core aldehydes (e.g., 9-oxo/10-oxo species), and representative –OOH/–OH OxTGs. Collectively, these findings clarify OxTG formation pathways and provide a practicable basis for risk-informed quality and safety control of frying oils.
在油炸过程中形成的氧化三甘油酯(oxtg)会降低油的质量,并引起安全问题。为了在类似煎炸的条件下确定氧的来源,采用双氢氧稳定同位素示踪设计(D2O, H218O, 18O2)对三油醇在180℃、3atm下加热0-4 h:天然空气(NA) + H2O、合成空气(SA) -16O2 + H218O和SA - 18O2 + D2O。诊断片段离子显示5种三酰基甘油和8种以过氧化氢(-OOH)和羟基(-OH)官能团为主的oxtg,而同位素比(M+ 1-M +4)/M定量标记合并。只有在SA-18O2 + D2O条件下才会发生明显的富集,特别是羟基化的OxTGs;NA + H2O和SA-16O2 + H218O的比例保持相对稳定,这与水主要作为过氧化氢转换和界面过程的动力学调节剂而不是oxtg的直接供氧体相一致。标记模式表明,氧结合到OxTGs主要来自分子氧(O2)。机械接地过程控制限制氧气的可用性,如真空或其他脱氧油炸,因此有望抑制氧化甘油的形成。为了便于决策监测和油周转率管理,建议使用综合指示面板,结合总极性化合物(TPC)、前驱甘油核心醛(例如,9-氧/10-氧)和代表性的-OOH / -OH OxTGs。总的来说,这些发现阐明了OxTG的形成途径,并为煎炸油的风险知情质量和安全控制提供了可行的基础。
{"title":"Dual H–O isotope tracing of oxidized triacylglycerols in triolein (180°C): mechanisms and control-oriented monitoring indicators","authors":"Yiting Duan ,&nbsp;Weidong Su ,&nbsp;Xinru Deng ,&nbsp;Chongyang Liu ,&nbsp;Qi Li ,&nbsp;Yuan Gao ,&nbsp;Xiuzhu Yu","doi":"10.1016/j.foodcont.2026.111972","DOIUrl":"10.1016/j.foodcont.2026.111972","url":null,"abstract":"<div><div>Oxidized triacylglycerols (OxTGs) formed during frying degrade oil quality and raise safety concerns. To resolve oxygen provenance under frying-like conditions, a dual hydrogen–oxygen stable-isotope tracing design (D<sub>2</sub>O, H<sub>2</sub><sup>18</sup>O, <sup>18</sup>O<sub>2</sub>) was applied to triolein heated at 180 °C for 0–4 h under 3 atm: natural air (NA) + H<sub>2</sub>O, synthetic air (SA)–<sup>16</sup>O<sub>2</sub> + H<sub>2</sub><sup>18</sup>O, and SA–<sup>18</sup>O<sub>2</sub> + D<sub>2</sub>O. Diagnostic fragment ions revealed five triacylglycerols and eight OxTGs dominated by hydroperoxide (–OOH) and hydroxyl (–OH) functionalities, while isotopologue ratios (M+1–M+4)/M quantified label incorporation. Pronounced enrichment occurred only under SA–<sup>18</sup>O<sub>2</sub> + D<sub>2</sub>O, particularly for hydroxylated OxTGs; ratios remained comparatively stable in NA + H<sub>2</sub>O and SA–<sup>16</sup>O<sub>2</sub> + H<sub>2</sub><sup>18</sup>O, consistent with water acting primarily as a kinetic modulator of hydroperoxide turnover and interfacial processes rather than as a direct oxygen donor to OxTGs.The labeling pattern demonstrates that oxygen incorporated into OxTGs derives primarily from molecular oxygen (O<sub>2</sub>). Mechanistically grounded process controls that limit oxygen availability—such as vacuum or otherwise deaerated frying—are therefore expected to suppress OxTG formation. For decision-ready surveillance and oil-turnover management, an integrated indicator panel is recommended, combining total polar compounds (TPC), sentinel glycerol-core aldehydes (e.g., 9-oxo/10-oxo species), and representative –OOH/–OH OxTGs. Collectively, these findings clarify OxTG formation pathways and provide a practicable basis for risk-informed quality and safety control of frying oils.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"184 ","pages":"Article 111972"},"PeriodicalIF":6.3,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Food Control
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