Food Control最新文献

{"title":"Developing sodium alginate based edible film incorporated with potassium sorbate and application for fresh cold pork preservation","authors":"Weiwei Jiang ,&nbsp;Xu Ding ,&nbsp;Ziting Zhang ,&nbsp;Wenhan Li ,&nbsp;Xuejin Li ,&nbsp;Lan Chen ,&nbsp;Yao Tang ,&nbsp;Yuqian Jiang","doi":"10.1016/j.foodcont.2025.111154","DOIUrl":"10.1016/j.foodcont.2025.111154","url":null,"abstract":"<div><div>Cold-stored fresh pork is widely consumed worldwide, whereas quality deterioration and spoilage limit its shelf-life and commercial value. The present study aims to develop a sodium alginate-based edible film incorporated with varying concentrations of potassium sorbate (PS) ranging from 0% to 5%, using 4% glycerol as a plasticizer. The optimal concentration of PS was determined to be 3% based on a comprehensive characterization of the film's optical, mechanical, and antimicrobial properties. The edible film containing 3% PS in a 2% sodium alginate substrate exhibited a uniform surface and well-arranged microstructure, with the film thickness of 0.43 mm, tensile strength (TS) of 7.11 MPa, elongation at break (EB) of 75.5%, and Water vapor permeability (WVP) of 1.33 g‧mm‧m⁻²‧h⁻¹‧kPa⁻¹. This formulation demonstrated superior tensile strength, elongation at break, and appropriate water vapor permeability. The preservation efficacy of the developed PS edible film on fresh pork was evaluated in comparison to PS solution spraying and dipping approaches. The results indicated that the PS edible film most effectively maintained the appearance, sensory attributes, and physiological quality of fresh pork during cold storage at 4 °C for 14 d. On the 14 d, the total volatile basic nitrogen (TVB-N) content was as low as 2.38 mg‧100 g⁻¹, the thiobarbituric acid reactive substances (TBARS) content was 0.63 mg‧kg⁻¹, the total viable count (TVC) was 5.12 log cfu‧g⁻¹, and the total count of molds and yeasts was 5.05 log cfu‧g⁻¹, which were significantly lower than those observed in the spraying and dipping treatments. These findings support the use of the PS edible film as an effective antimicrobial packaging solution, offering significant potential for preserving the quality of cold-stored fresh pork and extending its shelf life.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111154"},"PeriodicalIF":5.6,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A portable smartphone sensing platform for simple, rapid, and visual detection of SO2 in food","authors":"Baoli Xue ,&nbsp;Chenyang Yue ,&nbsp;Hongxia Tan ,&nbsp;Fangkai Du ,&nbsp;Lirong Jiang ,&nbsp;Lintao Zeng","doi":"10.1016/j.foodcont.2025.111165","DOIUrl":"10.1016/j.foodcont.2025.111165","url":null,"abstract":"<div><div>Excessive sulfur dioxide (SO₂) and its derivatives (HSO₃⁻/SO₃²⁻) in food pose a serious threat to human health. This study aimed to design a butterfly-shaped fluorescent probe DCMN to enhance food safety. The probe exhibited a chromogenic and fluorescence “turn-on” dual-mode response to HSO₃⁻. Furthermore, the probe was fabricated into a paper-based chip and a portable smartphone-adaptable sensing platform for simple, rapid, and visual detection of SO₂ in food. The sensing platform exhibited excellent performance in detecting HSO₃⁻, including high selectivity, fast response (&lt;6 s), and a low detection limit (11.4 nM). The sensing platform could replace sophisticated instruments by combining smartphone image recognition and paper-based sensing chips with mobility, flexibility, and intelligence. This smartphone sensing platform was successfully applied for detecting HSO₃⁻ in sugar, vermicelli, dried bean curd, and tremella with high sensitivity and good recovery (92.10%–107.00%). Therefore, this sensing platform can serve as a simple, low-cost, and portable device for rapid, intuitive on-site detection of sulfite in food.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111165"},"PeriodicalIF":5.6,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxins status in Morocco during the period 2011–2024: Monitoring in food and feed, risk assessment and regulation aspects","authors":"Mourad El Youssfi ,&nbsp;Samira El Akhdari ,&nbsp;Mounaim-Halim El Jalil ,&nbsp;Abdelaziz Laghzizil ,&nbsp;Aicha Sifou ,&nbsp;Abdelrhafour Tantaoui-Elaraki ,&nbsp;Abdellah Zinedine","doi":"10.1016/j.foodcont.2025.111151","DOIUrl":"10.1016/j.foodcont.2025.111151","url":null,"abstract":"<div><div>Mycotoxins issues continue to attract the interest of scientists and decision-makers worldwide given their harmful effects on human and animal health, and their economic impact. In Morocco, a Mediterranean and North African country, mycotoxins may constitute a potential risk for consumers and numerous research papers have been published during the last decade to assess the situation. Thus, the main objectives of this literature review are to provide the scientific community and Moroccan decision-makers with recent data and general overview of mycotoxin contamination over the period (2011–2024), to highlight the main contaminated food and feed, the most mycotoxins found and their contamination levels. Published data showed that the most studied food commodities in the country were cereals and their derivatives, spices, animal feed, dried fruits, medicinal plants, green tea and milk. The prevalence of some hazardous mycotoxins such as aflatoxins, ochratoxin A, deoxynivalenol, zearalenone and enniatins was found sometimes high in some investigated commodities (up to 93%). The regulatory aspects recently adopted in the country regarding mycotoxins will also be detailed, in comparison with the international legislation in the European area, as well as with the Codex Alimentarius regulations. Finally, the main studies on exposure assessment and risk assessment will also be discussed.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111151"},"PeriodicalIF":5.6,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm potential of cumin and lemongrass essential oils against multidrug-resistant Vibrio parahaemolyticus in retailed fish samples","authors":"Amnah A. Alasgah ,&nbsp;Ghada E. Ahmed ,&nbsp;Rasha M. El Bayomi ,&nbsp;Abdallah Fikry A. Mahmoud ,&nbsp;Ahmed S. El-tahlawy ,&nbsp;Karima M.E. Abdallah ,&nbsp;Mohamed T. Elabbasy ,&nbsp;Usama M. Ibrahem ,&nbsp;Alaa Eldin M.A. Morshdy","doi":"10.1016/j.foodcont.2025.111162","DOIUrl":"10.1016/j.foodcont.2025.111162","url":null,"abstract":"<div><div>The rising prevalence of antibiotic-resistant bacteria and biofilm formation in seafood presents significant public health risks. This study examined 90 fish samples, including brush tooth lizard (<em>Saurida undosquamis</em>), red porgy (<em>Pagrus pagrus</em>), and mullet (<em>Mugil cephalus</em>) (30 of each), to investigate the anti-biofilm properties of essential oils derived from cumin and lemongrass against <em>V. parahaemolyticus</em> at varying temperatures (4 °C and 37 °C). Additionally, the study assessed virulence-encoded genes in <em>V. parahaemolyticus</em> and the susceptibility of <em>Vibrio</em> isolates to antimicrobial agents. Bacteriological analysis identified 46 (51.1%) <em>Vibrio</em> species. Among these isolates, 36.8% carried the virulence-associated genes <em>tdh</em>, <em>trh</em>, and <em>tlh</em>. Antibiotic sensitivity testing revealed significant resistance among the isolates, with an average multiple antibiotic resistance (MAR) index of 0.473. <em>V. parahaemolyticus</em> showed markedly increased biofilm formation at 37 °C compared to 4 °C, highlighting a substantial temperature-dependent difference in production. Furthermore, lemongrass essential oil (LEO) and cumin essential oil (CEO) effectively inhibited <em>V. parahaemolyticus</em> biofilm production at sub-minimum inhibitory concentrations. These findings underscore the importance of regularly monitoring seafood for <em>Vibrio</em> species due to their antibiotic resistance, unique molecular traits, and biofilm-forming ability, all of which are critical for improving seafood safety. The demonstrated efficacy of LEO and CEO in inhibiting biofilm formation also presents a promising strategy for the food industry to enhance the safety and shelf-life of seafood products, potentially reducing the reliance on conventional antibiotics and addressing the growing challenge of antibiotic resistance. Overall, the study demonstrated that LEO and CEO could feasibly inhibit <em>V. parahaemolyticus</em> biofilm production and enhance the safety of consumed fish.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111162"},"PeriodicalIF":5.6,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving mango cold-damage and bruise detection using thermal imaging and flexible spectral sensing","authors":"Wenhao He ,&nbsp;Wentao Huang ,&nbsp;Tomo Popovic ,&nbsp;Zhiqiang Zhu ,&nbsp;Xiaoshuan Zhang","doi":"10.1016/j.foodcont.2025.111163","DOIUrl":"10.1016/j.foodcont.2025.111163","url":null,"abstract":"<div><div>Influenced by chemical processes such as respiration, ethylene production, and oxidation reactions, fruits are prone to cold damage and bruising during storage and transportation. To this end, this paper presents an ensemble learning network based on dual-modal data fusion for real-time detection and assessment of mango quality. On one hand, we developed a non-destructive classification system that integrates thermal imaging technology with flexible visible/near-infrared spectral sensing, capturing the physicochemical characteristics of mangoes both on the surface and internally. On the other hand, the CNN-ATT-BiLSTM-based ensemble learning framework effectively enables data fusion from the feature layer to the decision layer. Results indicate that mangoes can be classified into four categories: bruised, cold-damaged, both cold-damaged and bruised, and normal. The CNN-ATT-BiLSTM network achieved an overall accuracy of 95.24%. Experiments on grading pipelines demonstrated the system's strong stability. This research contributes new methodologies to the development of fruit quality assessment technologies.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111163"},"PeriodicalIF":5.6,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in green halochromic smart film packaging: Investigating the natural colorant as biosensor in smart film packaging for real-time meat freshness monitoring","authors":"Najma Farhaten Latiff ,&nbsp;Mannur Ismail Shaik ,&nbsp;Nizaha Juhaida Mohamad ,&nbsp;Wan Mohd Khairul ,&nbsp;Adibah Izzati Daud ,&nbsp;Bambang Nurhadi ,&nbsp;Norizah Mhd Sarbon","doi":"10.1016/j.foodcont.2025.111157","DOIUrl":"10.1016/j.foodcont.2025.111157","url":null,"abstract":"<div><div>Increasing awareness of maintaining meat freshness and environmental concerns has led to the innovation of film packaging. Introducing the smart film, which utilizes natural colorants, biodegradable polymers, and nanoparticles, enables a practicable approach to maintaining meat freshness as it offers real-time monitoring of meat spoilage and informs consumers of any alterations in the packaging environment. This review provides an overview of the current research on halochromic smart films incorporating natural colorants to biodegradable film packaging in reducing the risk of meat spoilage. The results from various studies demonstrate that halochromic properties are possessed by natural colorants, enabling the use of natural colorants as biosensors in smart film. Biosensors such as anthocyanin, betalains, and curcumin are reliable tools for detecting meat spoilage as they are non-toxic and sensitive to pH changes. Anthocyanin has been the most preferred biosensor due to its high sensitivity to pH changes and abundant availability. Moreover, incorporating nanoparticles in the smart film enhances the sensitivity of biosensors toward pH changes. Future studies should consider essential elements, including consumer acceptance, cost, and regulatory issues, to improve the properties of smart films for commercial applications.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111157"},"PeriodicalIF":5.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic and organic constituent analysis: A data fusion strategy to differentiate between wines of different origins","authors":"Yingyue Su ,&nbsp;Zhaoxiang Wang ,&nbsp;Qianqian Liu ,&nbsp;Yuyu Li ,&nbsp;Cui Zhang ,&nbsp;Xiaohui Feng ,&nbsp;Ang Zhang ,&nbsp;Chunlong Yuan","doi":"10.1016/j.foodcont.2025.111150","DOIUrl":"10.1016/j.foodcont.2025.111150","url":null,"abstract":"<div><div>The authenticity of wine is a significant concern in the global market due to widespread fraudulent activities involving wine origins. In this study, the inorganic and organic constituent profiles of 102 wine samples from five different growing regions were analyzed. Low- and mid-level data fusion coupled with a multivariate analysis approach was applied to three platforms of mass spectrometry data sets using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), Isotopic Ratio Mass Spectrometry (IRMS), and Ultra-high Performance Liquid Chromatography coupled with Ion Mobility Quadrupole Time-Of-Flight Mass Spectrometry (UPLC/IM-QTOF-MS) to determine the correct classification of wine origin. Significant difference of organic and inorganic compounds from five regions were selected for low- and mid-level data fusion. In the low-level data fusion, Partial Least Squares Discriminant Analysis (PLS-DA) under the positive ionization modes (ESI+) achieved an optimal prediction rate of 74.4%. By extracting features from low-level data fusion for mid-level data fusion, linear discriminant and nonlinear models were established, resulting in an optimal accuracy of 98.7%. The mid-level data fusion multivariate analysis strategy significantly enhanced the accuracy of identifying the geographical origin of wine and presents a new approach for identifying origins in the origin of other foods.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111150"},"PeriodicalIF":5.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early detection of Blossom-End Rot in green peppers using fluorescence and normal color images in visible region","authors":"Zichen Huang ,&nbsp;Tetsuyuki Takemoto ,&nbsp;Ken Abamba Omwange ,&nbsp;Michela Orsino ,&nbsp;Keiji Konagaya ,&nbsp;Naoshi Kondo","doi":"10.1016/j.foodcont.2025.111156","DOIUrl":"10.1016/j.foodcont.2025.111156","url":null,"abstract":"<div><div>Green peppers, a globally renowned vegetable known for their nutritional value, frequently encounter Blossom-End Rot (BER), a prevalent issue causing postharvest losses. Despite its significance, limited research has addressed the classification of BER-affected fruits from healthy ones. This study introduces a fluorescence imaging-based sensing approach designed to detect the presence of BER areas within captured fluorescence and color images of green peppers. Conducted across a two-year span in three Japanese greenhouses, this research focused on gathering BER-affected green pepper samples and characterizing their features. Utilizing a spectrofluorometer and fluorescence microscope, distinct characteristics of BER areas were estimated in comparison to healthy regions. A total of 774 image sets, each comprising images captured under various light sources, were analyzed. The findings revealed pronounced cell death and chlorophyll degradation within the BER areas, notably manifesting considerable changes in the G channel of images illuminated by white and 365 nm light sources. A classification model trained on these paired images achieved a 94.9% accuracy, including 86.2% for early-stage BER detection. Compared to the conventional ResNet50-based method, which struggled to detect subtle early-stage BER symptoms, the proposed method demonstrated superior performance. This dual-channel sensing approach offers a cost-effective and practical solution for accurately detecting BER at all stages, providing valuable applications for quality control in grading facilities and on farms.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111156"},"PeriodicalIF":5.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, antimicrobial, antibiofilm and insecticidal enhancing of Eucalyptus citriodora essential oil and its potential to shelf-life extension of pumpkin after inoculation of Salmonella enterica","authors":"Miroslava Kačániová ,&nbsp;Milena D. Vukic ,&nbsp;Nenad L. Vukovic ,&nbsp;Margarita Terentjeva ,&nbsp;Zhaojun Ban ,&nbsp;Li Li ,&nbsp;Alessandro Bianchi ,&nbsp;Rania Ben Saad ,&nbsp;Anis Ben Hsouna ,&nbsp;Joel Horacio Elizondo-Luévano ,&nbsp;Natália Čmiková ,&nbsp;Stefania Garzoli","doi":"10.1016/j.foodcont.2025.111152","DOIUrl":"10.1016/j.foodcont.2025.111152","url":null,"abstract":"<div><div>Our study aims to investigate the chemical composition and to evaluate the antibacterial (i<em>n vitro, in situ</em>) and insecticidal activities of <em>E. citridiora</em> essential oil (ECEO), given the current emphasis on the use of natural resources, particularly essential oils, in food production to improve sensory attributes and prolong shelf-life. In addition, microbiological studies of pumpkin sous vide treated with ECEO after being inoculated with <em>Salmonella enterica</em> were evaluated at one and seven days of the trial. GC/MS analysis of the examined essential oil showed the presence of 30 volatile compounds (92.7% of the total). Monoterpene aldehyde citronellal (65.1%), as well as monoterpene alcohols isopulegol (8.3%), citronellol (6.5%) and iso-isopulegol (4.2%) are the most abundant compounds. Measured using the disc diffusion method, the antibacterial activity of the EO ranged from 5.67 mm for <em>Candida albicans</em> to 17.67 mm for <em>Bacillus subtilis</em> subsp. <em>Spizizenii</em>. The lowest minimal inhition concentration was found against <em>S. epidermidis</em> and <em>Enterococcus faecalis</em> (MIC₅₀ 0.140 and 0.155 mg/mL and MIC₉₀ 0.177 and 0.184 mg/mL). In the vapor phase, the best antibacterial activity was shown against <em>S. epidermidis</em> in the strawberry model, <em>Yersinia enterocolitica</em> in the apricot model, <em>S. enterica</em> forming biofilms in the cucumber model and <em>S. epidermidis</em> in the pumpkin model. The growth of <em>S. enterica</em> was inhibited and the microbial count was decreased by using the sous vide method in combination with ECEO treatment. The bacteria <em>Bacillus amyloliquefaciens</em> subsp. <em>plantarum</em>, <em>Bacillus licheniformis</em>, <em>Bacillus safensis, Acinetobacter calcoaceticus</em>, <em>Pseudomonas ludensis</em>, and <em>Rhizobium radiobacter</em> were the most isolated microbiota found in the sous vide pumpkin. In our study, ECEO was found to significantly affect the degradation of <em>S. enterica</em> biofilm. It is first assessment of insecticidal activity of EO against <em>Megabruchidius dorsalis</em> which was sensitive to the ECEO. According to our knowledge, the antimicrobial and insecticidal activity demonstrated in our ECEO study suggests that it may have a rationale for application in the food sector as an additive to prolong the preservability of processed foods and has good potential for use as a natural pesticide.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111152"},"PeriodicalIF":5.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol mechanisms and antimicrobial gene expression of Bacillus 63–10 and impact on microbial ecosystems of fruit; quality and shelf life","authors":"Patumporn Manowan ,&nbsp;Hoang Truc Anh To ,&nbsp;Cheunjit Prakitchaiwattana","doi":"10.1016/j.foodcont.2025.111160","DOIUrl":"10.1016/j.foodcont.2025.111160","url":null,"abstract":"<div><div>Postharvest fruit deterioration due to microbial spoilage presents significant economic challenges in agricultural countries. Biocontrol has become a widely developed and applied method for fruit preservation. This study investigated the genes associated with antimicrobial agent synthesis in <em>Bacil</em>lus 63–10 and its inhibitory mechanisms when used as a biocontrol agent on fruit surfaces, using lychee as a model. Whole genome sequencing (WGS) revealed that <em>Bacil</em>lus 63–10 is closely related to <em>Bacillus velezen</em>sis and <em>Bacillus amyloliquefaci</em>ens, exhibiting non-pathogenic characteristics and having no resistance genes. End-point PCR testing identified five antimicrobial genes; <em>b</em>aeA, <em>b</em>acA, <em>f</em>enD, <em>sr</em>fAA, and Uni, responsible for antimicrobial activity against spoilage microbes in vitro. <em>Bacil</em>lus 63–10 coated onto fresh lychee surfaces showed significantly lower levels of spoilage bacteria (&lt;1.00–0.83 log CFU/cm²) and yeast and mold (1.63–1.95 log CFU/cm²) from days 3–7, compared to untreated lychees, which exhibited 2.16–7.06 and 2.67–4.00 log CFU/cm², respectively. The application of <em>Bacil</em>lus 63–10 on fresh lychee fruits helped preserve their physicochemical properties, including improved pericarp color, reduced disease incidence, and minimized weight loss, effectively extending the shelf life by more than 3 days. Gene expression analysis of lychee pericarp revealed dynamic patterns, <em>b</em>aeA was detected daily, <em>b</em>acA on day 2, and <em>f</em>enD on days 3 and 4. These expression patterns correlated with the types and numbers of spoilage microbes on the fruit's surface. The expression of these genes suggests that <em>Bacil</em>lus 63–10 adapts to the presence of spoilage microbes, effectively reducing their populations. These findings provide key insights into biocontrol mechanisms that can be optimized for developing effective biocontrol strategies. This study also demonstrates the potential of <em>Bacil</em>lus 63–10 as an effective biocontrol agent for preserving fresh fruits and reducing postharvest losses.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111160"},"PeriodicalIF":5.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}