Food Control最新文献

{"title":"Controlling Listeria monocytogenes contamination in fresh goat milk cheeses: Dynamic modelling during storage","authors":"Olga María Bonilla-Luque ,&nbsp;Arícia Possas ,&nbsp;Úrsula Gonzales-Barron ,&nbsp;Vasco Cadavez ,&nbsp;Youssef Ezzaky ,&nbsp;Abdelraheem Hussein ,&nbsp;Antonio Valero","doi":"10.1016/j.foodcont.2025.111194","DOIUrl":"10.1016/j.foodcont.2025.111194","url":null,"abstract":"<div><div>Predictive models applied for artisanal fresh goat cheeses are invaluable tools for rapid decision-making in quality and safety management. <em>Listeria monocytogenes</em> is a common foodborne pathogen associated with recent safety alerts in ready-to-eat (RTE) products in Andalucia. Managing artisanal production variables, such as storage conditions and final product features (pH or water activity [a_w]) might be crucial for controlling <em>L. monocytogenes</em> in these traditional products. This study evaluated the time-temperature dependence of <em>L. monocytogenes</em> during shelf-life of fresh goat milk cheese artisanally produced in Andalucía. The effect of storage temperature (4–25 °C) over 5–20 days was tested in lab-scale fresh goat milk cheeses initially inoculated with a three-strain cocktail of the pathogen (ca. 2–3 log cfu/g). The Huang (model A) and Baranyi (model B) models coupled with Ratkowsky were fitted to the collected growth data to describe the relationship between <em>L. monocytogenes</em> concentration, growth rate (<em>μ</em>_max, log cfu/d), storage temperature, and time. The results highlighted the higher variability of growth data at 4 °C, the linear relationship between <em>μ</em>_<em>max</em> and temperature, the inverse correlation between y_<em>max</em> (exceeding 5 log cfu/g at all the temperatures) and <em>ΔY</em> (with a maximum of 4.386 and minimum of 2.461, at 4 and 25 °C, respectively) and a faster decrease of pH at higher temperatures. Model validity, assessed through goodness-of-fit and both internal and external validations (under both isothermal temperatures and a dynamic temperature workflow simulating real production scenario), revealed good model adjustment to <em>L. monocytogenes</em> growth in artisanal commercial cheeses, after determining a correction factor of 1.806 and 1.856, for models A and B, respectively. Predictions indicated that an initially contaminated artisanal fresh goat milk cheese would not meet regulatory limits after 4 days under refrigeration conditions (4 °C), aerobic conditions, or opened packaging. These results highlight the potential public health risk of artisanal fresh goat milk cheeses related to <em>L. monocytogenes</em> transmission if manufacturing and storage conditions are not well controlled, emphasizing the need for stringent microbial control efforts and mitigation measures.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"174 ","pages":"Article 111194"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate change and campylobacteriosis from chicken meat: The changing risk factors and their importance","authors":"Kevin Queenan,&nbsp;Barbara Häsler","doi":"10.1016/j.foodcont.2025.111193","DOIUrl":"10.1016/j.foodcont.2025.111193","url":null,"abstract":"<div><div>Globally, chicken meat is currently the highest consumed meat per capita, and it continues to rise. Campylobacteriosis is one of the most reported gastrointestinal conditions, typically associated with chicken meat consumption. Cases are seasonal with summer and early autumn peaks. Similar seasonal peaks in <em>Campylobacter</em> prevalence in broilers and in retailed chicken meat have also been shown. Climate change impacts include increased ambient temperatures, rainfall, and humidity, and more frequent extreme weather events. These are likely to impact the risks associated with warmer-season foodborne diseases like campylobacteriosis. A literature review was conducted to identify the chicken related <em>Campylobacter</em> risk factors from farm to fork. Expert opinion was gathered using a modified Delphi survey in two rounds: 1) to identify risk factors whose likelihood of occurring would be impacted by climate change, 2) to determine the likelihood of the proposed change and the impact on campylobacteriosis from chicken meat consumption. Likert scores were used to calculate a mean risk level value. The latter was used together with a respondent agreement cut-off of over 66% to highlight risk factors most likely to change and to impact the risk of campylobacteriosis from chicken meat under climate change. Increasing temperatures and humidity and the extension of summer and early autumn seasons had the overall highest Mean Risk Level value (19/25). The increased prevalence of pests, especially flies had the second highest (16/25), and the highest respondent agreement level (94%). Several water-related risk factors were found likely to increase, including water drinker contamination, use of non-mains water sources, and those associated with water-based broiler house hygiene. Heat stress related risks were also highlighted, including the increased likelihood of on-farm <em>Campylobacter</em> positive animals and recent in-flock mortalities, a high degree of caecal colonisation, and faecal shedding of <em>Campylobacter,</em> and contaminating drinkers and carcase washing water. Other risk factors affected included higher consumption volumes and frequency of chicken meals, and broiler farmers having under 10 years of experience. These findings provide insights on how climate change may affect risk factor occurrence in the future and highlights those risks that decisionmakers should consider more closely in the future.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"173 ","pages":"Article 111193"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary survey on the presence of ciguatoxins (CTXs) in fishery products sold in Italy: An initial step towards the proper assessment of an emerging EU risk","authors":"Marta Di Maggio ,&nbsp;Christopher R. Loeffler ,&nbsp;Lara Tinacci ,&nbsp;Elena Varinelli ,&nbsp;Sonia Dall’Ara ,&nbsp;Synne T. Frøstrup ,&nbsp;Astrid Spielmeyer ,&nbsp;Alice Giusti ,&nbsp;Andrea Armani","doi":"10.1016/j.foodcont.2025.111202","DOIUrl":"10.1016/j.foodcont.2025.111202","url":null,"abstract":"<div><div>Ciguatera poisoning is a food-borne disease related to the consumption of finfish from areas endemic for ciguatoxins (CTXs). Mislabeling of fishery products can enhance consumer's exposure to this hazard by hiding the presence of CTXs vector species. In this study, we identified the main CTXs vector fish families (CVF) and the FAO areas at CTXs risk. Market products from CVF and sourced from CTXs risk areas were collected in Italy, molecularly authenticated to detect potential mislabeling cases, and then analysed for CTXs using a two-tier detection method (cell-based assay and mass spectrometry). Additionally, the official lists of seafood trade names (OL) from EU countries associated with ciguatera poisoning (CP) outbreaks linked to imported products were evaluated to assess a possible correlation between market species and CP risk. The Italian OL was also included. A total of 24 CVF and four FAO areas were selected. Overall, 51 market products were collected. The availability of CVF products from FAO areas at CTXs risk was limited in the Italian market. Molecular authentication revealed 16 cases of mislabeling (31.4%) in products from Sparidae, Lethrinidae and Epinephelidae. All samples analysed tested negative for CTXs. The OL analysis highlighted that a revision to improve correspondence between trade names and species would be beneficial also in the light of a proper risk assessment. Although no CP outbreaks have been reported in Italy by far, and no CTXs were detected in this study, the implementation of targeted programs to prevent and manage CP risk should be considered. This is particularly important because i) mislabeling remains prevalent in products imported to Italy, and ii) future indigenous outbreaks due to climate changes should be anticipated.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111202"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and verification of GC-MS/MS method for four fluoroquinolones residues determination based on precolumn derivatization: Application to poultry eggs","authors":"Hao Ding ,&nbsp;Junjie Xu ,&nbsp;Shuyu Liu ,&nbsp;Pengfei Gao ,&nbsp;Yawen Guo ,&nbsp;Yang Cao ,&nbsp;Yiwei Hong ,&nbsp;Xing Xie ,&nbsp;Tao Zhang ,&nbsp;Genxi Zhang ,&nbsp;Xiaodong Guo ,&nbsp;Guojun Dai ,&nbsp;Maoda Pang ,&nbsp;Kaizhou Xie","doi":"10.1016/j.foodcont.2025.111203","DOIUrl":"10.1016/j.foodcont.2025.111203","url":null,"abstract":"<div><div>In this study, liquid‒liquid extraction and solid‒phase extraction were coupled with derivatization to detect 4 types of fluoroquinolones residues in poultry eggs (chicken, duck, goose, and quail eggs) via GC-MS/MS. A 10% trichloroacetic acid‒acetonitrile solution and trimethylsilyl diazomethane (TMSD) were chosen as the extraction and derivatization reagents, respectively. The results revealed that the detection and quantitative limits (LODs, LOQs) were 1.0, 0.2, 0.5, and 1.0 μg/kg and 2.0, 0.4, 1.0, and 2.0 μg/kg, respectively. The peak areas of all the target compounds had a good linear relationship with the corresponding concentrations of the matrix matched standard working solution, with R² values higher than 0.9991. The recoveries of the target compounds were all higher than 78.0%. The RSDs ranged from 2.1% to 8.0%. All the matrices had weak matrix effect. This established method satisfied the requirements of the NY/T 1896–2010 industry standard for veterinary drug residue detection in China, providing a scientific basis for fluoroquinolone residue detection. Furthermore, this method expands the monitoring range, improves the detection efficiency, reduces the cost, and meets the detection needs of different laboratories.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111203"},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review for carbon dots-based fluorescent sensing tools for antibiotic and pesticide residues progress, challenge and perspective","authors":"Jiaxin Zheng,&nbsp;Zimin Lian,&nbsp;Ting Liu,&nbsp;Min Ouyang,&nbsp;Shali Jiang,&nbsp;Xiaomin Yuan,&nbsp;Liyi Zhou","doi":"10.1016/j.foodcont.2025.111201","DOIUrl":"10.1016/j.foodcont.2025.111201","url":null,"abstract":"<div><div>The abuse of antibiotics and pesticides results in their susceptibility to residues in the environment and food, thus it is significant essential to develop more portable and effective methods for the rapid detection of them. As a new type of fluorescent nanomaterials with unique optical properties, carbon dots (CDs) play an important role in various fields, especially their potential in environmental monitoring and food safety detection, and show a broad application prospect. In this work, we systematically reviewed the research progress on the latest applications of CDs-based fluorescence detection methods in antibiotic and pesticide residue assays and summarized the advantages and challenges of CDs in these fields. In addition, discussed the future directions of development, which will serve as a reference for further research on CDs in antibiotic and pesticide detection.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111201"},"PeriodicalIF":5.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection of five potentially allergenic tropical fruit species (mango, pineapple, avocado, banana, and coconut) using multiplex polymerase chain reaction coupled with capillary electrophoresis","authors":"Jae-Eun Cha,&nbsp;Seung-Man Suh,&nbsp;Inae Lee,&nbsp;Hae-Yeong Kim","doi":"10.1016/j.foodcont.2025.111199","DOIUrl":"10.1016/j.foodcont.2025.111199","url":null,"abstract":"<div><div>In this study, a multiplex polymerase chain reaction (PCR) assay that can simultaneously and sensitively detect the presence of five potentially allergenic tropical fruit species was developed to prevent allergic reactions among consumers and enhance future regulations on food allergy labeling. The novel species-specific primer set was designed with high specificity and sensitivity so that it can be applied even in processed foods. The primer set targets chloroplast genes <em>matK</em> and ITS regions. The obtained amplicon sizes were 114 bp for mango, 132 bp for pineapple, 158 bp for avocado, 183 bp for banana, and 242 bp for coconut. The specificity of the multiplex PCR assay was validated with 27 different plant species including five tropical fruit species, demonstrating no cross-reactivity and confirming detection sensitivity at approximately 1 pg. Its applicability was further verified using 32 commercially available food products. In particular, juice products containing two distinct fruit types displayed amplification signals for both species, highlighting the method's ability to detect multiple species simultaneously.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111199"},"PeriodicalIF":5.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme inactivation in broccoli of different sizes and densities using a pilot-scale 27.12 MHz radio frequency heating system","authors":"Xiangyi Wang ,&nbsp;Yandi Zeng ,&nbsp;Jie Hu ,&nbsp;Zhongxiang Zhou ,&nbsp;Shaojin Wang ,&nbsp;Qian Hao ,&nbsp;Zhi Huang","doi":"10.1016/j.foodcont.2025.111192","DOIUrl":"10.1016/j.foodcont.2025.111192","url":null,"abstract":"<div><div>This study aimed to explore how cutting size affects the efficiency and effectiveness of radio frequency (RF) blanching in broccoli, a vegetable with an irregular shape. Using a 6 kW, 27.12 MHz RF system, broccoli samples (divided into whole florets, quartered pieces, and bud florets) were treated at different electrode gaps (90, 95, 100, 105, and 110 mm) and target temperatures (70, 80, and 90 °C). The goal was to identify the optimal blanching conditions by assessing the blanching rate and uniformity based on peroxidase (POD) activity following treatments with RF alone, hot water (HW) alone, and the combined RF + HW method. The results showed that both the temperature and duration of RF heating had a significant impact on POD activity, emphasizing the need for precise thermal control. The combined RF + HW approach proved to be more efficient than HW blanching alone, resulting in reduced blanching times by 30.0%, 50.8%, and 32.3% for whole florets, quartered pieces, and bud florets, respectively. It also minimized vitamin C loss by 22.0%, 14.3%, and 21.7%, and reduced weight loss to 62.5%, 41.1%, and 57.1% of the values observed with HW blanching. Additionally, energy efficiency increased by 120.0%, 113.3%, and 103.1%, and reductions in hardness were lower at 50.1%, 13.5%, and 17.6%. In conclusion, the RF + HW technique enhances blanching efficiency and preserves product quality across various broccoli cut sizes. This combined approach shows great promise for large-scale industrial applications by reducing energy consumption and improving nutrient retention.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111192"},"PeriodicalIF":5.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to Aflatoxins and Ochratoxin A From the Consumption of Selected Staples and Fresh Cow Milk in the Wet and Dry Seasons in Ghana.","authors":"Abdul Hamid Nuhu, Winfred-Peck Dorleku, Beatrice Blay, Evans Derban, Clara Owusuwah McArthur, Simon Elikplim Alobuia, Araba Incoom, Derry Dontoh, Isaac Williams Ofosu, Daniel Oduro-Mensah","doi":"10.1016/j.foodcont.2024.110968","DOIUrl":"10.1016/j.foodcont.2024.110968","url":null,"abstract":"<p><p>Across sub-Saharan Africa, the heavy reliance on mycotoxin-susceptible staple foods means that populations in the region are particularly vulnerable to chronic mycotoxin exposure. This study assessed the exposure risk to ochratoxin A (OTA) and aflatoxins (AFs) from 18 samples of selected staple foods (maize, millet, groundnut) and 56 fresh cow milk samples collected from across Ghana. The foods were sampled simultaneously to maximise comparability, and at two timepoints in March/April (during the dry season) and July/August (during the rainy season) to assess the effects of duration of storage and seasonal conditions on the mycotoxin levels as measured by high-performance liquid chromatography. The margin of exposure (MOE) approach was used to assess the exposure risk from consumption of the sampled foods. Each of the sampled staples was contaminated with OTA (0.19-3.11 ng/g) and at least one AF (0.75-13.05 ng/g B₁, ND-12.12 ng/g B₂, 0.1-9.95 ng/g G₁, ND-16.78 ng/g G₂). Up to 67% had contamination above European Food Safety Authority (EFSA) maximum limits, and 50% were above Ghana Standards Authority (GSA) limits. The fresh cow milk samples were contaminated with AFM1 in the range of 0.05-1.49 ng/g, with 95% above EFSA limits and 36% above GSA limits. Aflatoxin contamination in the staples was high, particularly in July/August when the wet conditions may have adversely impacted the handling and storage of farm produce. Variation in AFM₁ between the two sampling periods mirrored total aflatoxin in the staples, suggesting that even if dairy cattle were grazing in open pasture and not being rationed on stored feed, then there was a high environmental presence of aflatoxigenic fungi. The MOE estimates were ≤ 533.09, far below the safe cut-off of 10,000 for suspected carcinogenic compounds. The high mycotoxin levels indicate a priority risk to child nutrition which relies heavily on cereal mixes based on one or all the three sampled staples. The data from this study underscore the urgent need for interventions to better appreciate and address mycotoxin exposure for enhanced food security and public health in Ghana and across sub-Saharan Africa.</p>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk allergen quantification in rice flour: An experimental investigation of sampling and testing procedures","authors":"Anders R. Andersen ,&nbsp;Charlotte B. Madsen ,&nbsp;Katrine L. Bøgh ,&nbsp;Ana I. Sancho","doi":"10.1016/j.foodcont.2025.111195","DOIUrl":"10.1016/j.foodcont.2025.111195","url":null,"abstract":"<div><div>Unintended allergen presence in foods may cause severe allergic reactions, thus posing a health risk for food allergic consumers. Consequently, accurate allergen quantification in foods is relevant for food allergic individuals, the food industry and authorities. However, regulation regarding sampling methods for food allergen analysis is lacking. The aim of the present study was to investigate the influence of the laboratory sample size and test portion size on food allergen analytical determinations by ELISA in an amorphous homogeneous milk contamination scenario.</div><div>A sampling plan was designed where seven lots of 1000 g rice flour contaminated with increasing amounts of skimmed milk powder (from 0 to 500 ppm milk protein contamination) were produced, and five 100 g as well as five 200 g laboratory samples were collected. From each laboratory sample, two test portions of 0.5 g and two test portions of 5 g were extracted, and two aliquots from each test portion were analysed for detection of milk allergens using a commercial ELISA kit. To evaluate the sampling plan, the measured concentration variability was estimated using a hierarchical mixed effects model, and the statistical significance of each sampling step was validated using permutation tests.</div><div>The experimental data indicated that the variance of the measured concentrations increased as a function of the spiked concentration. While increasing the test portion size from 0.5 to 5 g did not improve test precision, increasing the laboratory sample size from 100 to 200 g improved the test precision by reducing the variance of the laboratory sample by 88% and the total variance by an average of 63%. Thus, when estimating producer's and buyer's risks, increasing laboratory sample size from 100 to 200 g greatly improved the precision compared to increasing sample number from one to two 100 g samples, which reduced the total variance by 50%.</div><div>The laboratory sample size was critical for the analytical accuracy of the sampling plan for detection of milk protein contamination in rice flour, which was used in this study as an example of an allergen contamination scenario, and for reducing the risks associated with sample misclassification.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111195"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143289719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hairpin reporters-based one-pot LAMP-CRISPR/Cas12a assay for the highly sensitive and visual detection of Vibrio parahaemolyticus","authors":"Jinmei Li ,&nbsp;Shoujia Lin ,&nbsp;Ziyue Lang ,&nbsp;Gaowa Xing ,&nbsp;Yanwei Jia ,&nbsp;Hongmei Cao","doi":"10.1016/j.foodcont.2025.111184","DOIUrl":"10.1016/j.foodcont.2025.111184","url":null,"abstract":"<div><div>Consumption of <em>Vibrio parahaemolyticus</em> (VP)-infected seafood can lead to illnesses such as acute gastroenteritis, wound infections, and sepsis. Early detection of VP is crucial for food safety and human health. In this study, we developed a hairpin beacon (HB) reporters-based CRISPR/Cas12a method combined with Loop-mediated Isothermal Amplification (LAMP) for the detection of the VP-<em>tlh</em> gene. Compared to the classical ssDNA reporters (FQ reporters), the HB reporters exhibited a higher fluorescence response signal and excellent stability in the LAMP-Cas12a system. Our assay achieved low LOD in 40 min, 1.32 copies/μL of plasmid DNA and 1.23 CFU/mL of pure cultures about VP. Moreover, the detection results can also be directly observed with the naked eye. Finally, proposed method demonstrated high specificity and sensitivity in the random detection of 36 actual samples compared to qPCR. Thus, proposed the highly sensitive and visual detection system is highly significant for rapid detection in resource-scarce environments.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111184"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}