Food Control最新文献_第4页

Evaluation of the prevalence of Paralytic Shellfish Toxins in non-traditional vectors and potential health risks associated to their consumption 评估麻痹性贝类毒素在非传统媒介中的流行程度以及与食用贝类毒素相关的潜在健康风险

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-08 DOI: 10.1016/j.foodcont.2025.111351

Verónica Rey , Araceli E. Rossignoli , Francisco Rodríguez , Juan Blanco , Soledad Garrido , Begoña Ben-Gigirey

Paralytic shellfish toxins (PSTs) are potent marine neurotoxins mainly associated with dinoflagellates. European Union (EU) Regulation Nº 853/2004 and its amendments establish the maximum permitted levels for PSTs in bivalve molluscs, echinoderms, tunicates and gastropods. In Europe, the analyses of marine biotoxins for monitoring purposes is mainly focused on bivalve molluscs. Other vectors, including some from the regulated groups, are rarely covered by monitoring programs.

The aim of this study is to estimate the prevalence of PSTs across species from eight marine invertebrate groups and fish in Galicia (NW Spain). In total, 533 invertebrates (echinoderms, gastropods, cnidarians, crustaceans, cephalopods, polychaetes, tunicates, porifera and bivalves) and 96 fish samples were collected between April 2021 and September 2023 and analysed using both LC-FLD and LC-MS/MS methods. PSTs were detected in 265 samples in all groups except cephalopods and tunicates. Quantifiable levels were found in 233 samples.

Our results show the existence of potential PST vectors other than bivalves, including edible organisms of great economic value. These findings could be extrapolated to other countries with similar harmful algae events and stress the need for further surveys on different groups of marine invertebrates for a better public health protection. To our knowledge, this is the first report of PSTs in Littorina spp., Nassarius spp., Patella ulyssiponensis, Balanus spp., Carcinus maenas, Luidia sarsi and Ophiotrix spp. in the EU. Moreover, PSTs are also described for the first time in Nucella spp., Patella spp. and Polybius henslowii from Spain.

麻痹性贝类毒素（PSTs）是一种主要与鞭毛虫有关的强效海洋神经毒素。欧洲联盟（欧盟）规例Nº853/2004及其修订订明双壳类软体动物、棘皮动物、被囊动物及腹足类动物体内PSTs的最高容许含量。在欧洲，用于监测目的的海洋生物毒素分析主要集中在双壳类软体动物上。其他病媒，包括一些来自受监管群体的病媒，很少被监测项目覆盖。本研究的目的是估计加利西亚（西班牙西北部）八个海洋无脊椎动物种群和鱼类中pst的患病率。在2021年4月至2023年9月期间，共收集了533种无脊椎动物（棘皮动物、腹足动物、刺胞动物、甲壳动物、头足动物、多毛类、被囊动物、多孔动物和双壳类）和96种鱼类样本，并使用LC-FLD和LC-MS/MS方法进行了分析。除头足类和被囊类外，其余各组265份样品均检测到PSTs。在233个样本中发现了可量化的水平。我们的研究结果表明，除了双壳类之外，存在潜在的PST载体，包括具有巨大经济价值的可食用生物。这些发现可以外推到其他有类似有害藻类事件的国家，并强调需要对不同的海洋无脊椎动物群体进行进一步调查，以更好地保护公众健康。据我们所知，这是欧盟首次报道Littorina spp、Nassarius spp、Patella ulyssiponensis、Balanus spp、Carcinus maenas、Luidia sarsi和Ophiotrix spp中存在PSTs。此外，在西班牙的Nucella spp.、Patella spp.和Polybius henslowii中也首次发现了pst。

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引用次数: 0

A ratiometric fluorescence biosensor based on enzyme-cascade signal amplification technology for the detection of lincomycin 基于酶级联信号放大技术的比例荧光生物传感器检测林可霉素

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-07 DOI: 10.1016/j.foodcont.2025.111344

Yiyao Liu, Menglong Liu, Jingyi Xiao, Yi Ren, Jinyang Li, Jiayan Gao, Litao Chen, Xue Gao

An enzyme-nanozyme cascade amplified ratio fluorescence (RF) biosensor based on prepared MnFe-layered double hydroxide nanosheets (Mn-Fe LDH) and alkaline phosphatase (ALP) has been developed for sensitive and accurate detection of lincomycin (LIN). In this strategy, Mn-Fe LDH can catalyze the o-phenylenediamine (OPD) oxidation to generate fluorescence 2, 3-diaminophenazine (DAP) with a fluorescence wavelength of 565 nm. This study utilized LIN aptamer-modified magnetic beads and alkaline phosphatase-labeled complementary strand hybridization to prepare the MBs-Apt@cDNA-ALP. The MBs-Apt@cDNA-ALP could recognize LIN and release cDNA-ALP from the surface of the magnetic beads. After magnetic separation, cDNA-ALP catalyzed the 2-phosphate ascorbic acid (AAP) conversion to ascorbic acid (AA). The redox reaction between AA and Mn-Fe LDH led to a decrease in DAP production and fluorescence intensity. At the same time, AA converted into dehydroascorbic acid (DHAA), which reacted with OPD to form a quinoline derivative (DFQ) that emitted fluorescence at 435 nm. The constructed LIN biosensor exhibited a detection range of 1.125–250 nM with a limit of detection (LOD) of 0.0492 nM. Moreover, the RF aptasensor can eliminate background interference, deliver remarkable signal variations, and effectively analyze the LIN concentration in grass carp and shrimp samples.

以制备的mnfe层状双氢氧化物纳米片（Mn-Fe LDH）和碱性磷酸酶（ALP）为材料，建立了一种酶-纳米酶级联放大比例荧光（RF）生物传感器，用于灵敏、准确地检测林可霉素（LIN）。在该策略中，Mn-Fe LDH可以催化邻苯二胺（OPD）氧化生成荧光2,3 -二氨基吩嗪（DAP），荧光波长为565 nm。本研究利用LIN适配体修饰磁珠和碱性磷酸酶标记互补链杂交制备MBs-Apt@cDNA-ALP。MBs-Apt@cDNA-ALP可以识别LIN并从磁珠表面释放dna - alp。经磁分离后，dna - alp催化2-磷酸抗坏血酸（AAP）转化为抗坏血酸（AA）。AA与Mn-Fe LDH之间的氧化还原反应导致DAP的生成和荧光强度下降。同时，AA转化为脱氢抗坏血酸（DHAA），与OPD反应生成喹啉衍生物（DFQ），在435 nm处发出荧光。所构建的LIN生物传感器检测范围为1.125 ~ 250 nM，检出限为0.0492 nM。此外，射频感应传感器可以消除背景干扰，提供显著的信号变化，有效地分析草鱼和虾样品中的LIN浓度。

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引用次数: 0

Inactivation of Penicillium spp. in apple juice by pulsed light and the exploration of its mechanisms 脉冲光对苹果汁中青霉的灭活及其机理的探讨

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-07 DOI: 10.1016/j.foodcont.2025.111343

Guolong Li , Saiqun Wang , Yu Li , Lu Cui , Rui Cai , Tianli Yue , Yahong Yuan , Zhouli Wang

Penicillium spp. is a crucial factor affecting fruit juice quality, so it is imperative to control their contamination. The objective of this study was to evaluate the efficiency of pulsed light (PL) treatments on the inactivation of Penicillium spp. in apple juice and its mechanisms. The effect of different processing parameters on the inactivation of Penicillium spp. was explored. After inoculation of 6.00 log CFU/mL of target fungi in 12 ^oBrix apple juice, the colony counts of all Penicillium spp. were below the quantification limit (1.00 log CFU/mL) at the PL total fluence of 81.00 J/cm². The Weibull model was most suitable for kinetic fitting of the inactivation process of patulin-producing fungi by PL treatment. Besides, the results showed PL treatment caused significant deformation and rupture of fungal spores, leading to the release of cell contents and ultimately inactivating Penicillium spp. through combined photophysical and photochemical effects. The PL treatment effectively inactivates Penicillium spp. in apple juice, providing an efficient method for bacterial control in fruit juices.

青霉菌是影响果汁质量的重要因素，对其进行污染控制势在必行。研究了脉冲光处理对苹果汁中青霉的灭活效果及其机制。探讨了不同工艺参数对青霉菌灭活的影响。在12株oBrix苹果汁中接种目标真菌6.00 log CFU/mL后，所有青霉菌菌落计数均低于定量限（1.00 log CFU/mL）， PL总影响为81.00 J/cm2。威布尔模型最适合于PL处理下产展霉素真菌失活过程的动力学拟合。此外，PL处理通过光物理和光化学的联合作用，引起真菌孢子的明显变形和破裂，导致细胞内容物释放，最终使青霉菌失活。PL处理能有效灭活苹果汁中的青霉，为果汁细菌控制提供了一种有效的方法。

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引用次数: 0

A novel coating formulated with sodium alginate loaded with a mixed lactic acid bacteria culture to control anthracnose in guava and mango 用海藻酸钠负载混合乳酸菌培养物配制一种新型涂层以控制番石榴和芒果的炭疽病

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-06 DOI: 10.1016/j.foodcont.2025.111341

Karina Felix Dias Fernandes , Kataryne Árabe Rimá de Oliveira , Ítallo Fernandes Cirilo , Maiara da Costa Lima , Marcos Paz Saraiva Câmara , Evandro Leite de Souza

Guava and mango are tropical fruits widely consumed due to their pleasant flavor and nutritional composition. Anthracnose caused by Colletotrichum spp. Is considered a primary post-harvest disease affecting these fruits. Applying biocontrol agents, such as lactic acid bacteria (LAB), combined with coating materials is an innovative fruit biopreservation method. This study investigated the effects of a novel sodium alginate coating loaded with a mixed culture of LAB in controlling anthracnose caused by different pathogenic Colletotrichum isolates in guava (cv.) Paluma and mango (cv.) Palmer during 15 days of room temperature storage (25 ± 0.5 °C). A mixed culture formed by three different LAB species strains (Levilactobacillus brevis 59, Lactiplantibacillus pentosus 129, and Limosilactobacillus fermentum 263) was tested against ten target Colletotrichum isolates. The mixed culture inhibited the mycelial growth of all target Colletotrichum isolates in vitro, with up to 100 % inhibition. In the nutrient competition method, the mixed LAB culture survived and caused mycelial growth inhibition even at low nutrient concentrations. The coatings showed sizes of LAB cell subpopulations with non-permeabilized membrane (viable cells, PI-CFDA+) of >31 % at the end of refrigerated storage and viable cell counts of >7 log CFU/g. Applying coatings with sodium alginate loaded with mixed LAB culture delayed the development and decreased the severity of anthracnose lesions in guava and mango artificially contaminated with either of the tested Colletotrichum isolates. The coating formulated with sodium alginate and mixed LAB culture can be effective for postharvest anthracnose biocontrol in guava and mango.

番石榴和芒果是热带水果，因其令人愉悦的风味和营养成分而被广泛食用。炭疽杆菌引起的炭疽病被认为是影响这些水果的主要采收后疾病。乳酸菌（LAB）等生物防治剂与包衣材料相结合是一种创新的水果生物保鲜方法。研究了一种新型海藻酸钠包衣负载混合培养的乳酸菌对番石榴不同病原菌炭疽菌引起的炭疽病的防治效果。棕榈和芒果（cv.）室温（25±0.5°C）保存15天。以3株乳酸菌（短乳酸杆菌59、戊酸乳酸杆菌129、发酵乳酸杆菌263）为混合培养物，对10株炭素菌进行了抑菌试验。混合培养对所有目标炭疽菌菌丝生长均有抑制作用，抑制率达100%。在营养竞争法中，即使在低营养浓度下，混合LAB也能存活并抑制菌丝生长。结果显示，在冷藏结束时，未渗透膜的LAB细胞亚群（活细胞，PI-CFDA+）的大小为31%，活细胞计数为7 log CFU/g。用海藻酸钠包覆混合LAB培养物可延缓被任一种炭疽病分离菌人工污染的番石榴和芒果炭疽病的发展，降低炭疽病的严重程度。以海藻酸钠和混合LAB培养配制的包衣对番石榴和芒果采后炭疽病有较好的生物防治效果。

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引用次数: 0

Beyond mislabelling: Chinese fish balls authentication by metabarcoding allows unveiling hidden mammal and avian species 除标签错误外：中国鱼丸通过元条形码认证，可以揭示隐藏的哺乳动物和鸟类物种

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-05 DOI: 10.1016/j.foodcont.2025.111338

Xia Zhang , Alice Giusti , Sihui Li , Weide Deng , Zhenzhu Sun , Yuan Li , Hongyuan Peng , Jiajie Hu , Andrea Armani , Jing Wen

In this study, 47 Chinese fish balls (FBs) sold on e-commerce platforms were collected and the ingredient of animal origin were identified using 16S rRNA metabarcoding. The sequencing results were compared to the FBs declared composition (DC) - obtained by combining the trade name and the ingredient list - to assess mislabelling. FBs were considered mislabelled if an ingredient not reported in the DC was detected molecularly, or if an ingredient in the DC was not detected. Excluding 14 FBs that only reported the generic term “fish" on the label, the DC mostly included eel (Anguilliformes), shark (Elasmobranch), and freshwater fish species. The presence of pork or chicken was also declared in a number of FBs. Overall, 65.9% of the FBs (n = 31) were mislabelled. The following cases were highlighted (the same FB could be included in more than one case, simultaneously): i) 21 FBs containing undeclared pork, chicken, or beef; ii) 9 FBs containing undelcared additional fish species; iii) 7 FBs not containing the fish reported in the DC. Threatened fish species were also detected. These mislabelling cases highlight the challenges consumers face in making ethical, religious, and sustainable choices. A more thorough evaluation of the quantitative potential of metabarcoding, as well as the possibility to combine this technique with quantitative methods (e. g. qPCR) should be considered for monitoring the surimi production chain.

本研究收集了 47 种在电子商务平台上销售的中国鱼丸（FBs），并使用 16S rRNA 代谢编码鉴定了其中的动物源成分。将测序结果与鱼丸的申报成分（DC）（由商品名称和配料表组合而成）进行比较，以评估贴错标签的情况。如果分子检测到成分表中未报告的成分，或成分表中未检测到的成分，则认为食品标签有误。剔除 14 款在标签上只报告了 "鱼 "这一通用术语的食品后，区域标准大多包括鳗鲡目、鲨鱼目和淡水鱼类。此外，亦有一些食物樣本聲稱含有豬肉或雞肉。总体而言，65.9%的食品标签（n = 31）被误标。重点关注的情况如下（同一食品标签可能同时包含多个情况）：i) 21 个食品标签含有未申报的猪肉、鸡肉或牛肉；ii) 9 个食品标签含有未申报的额外鱼类物种；iii) 7 个食品标签不含区级食品中心报告的鱼类。此外，还发现了受威胁的鱼类品种。这些错误标签案例凸显了消费者在做出道德、宗教和可持续选择时面临的挑战。在监控鱼糜生产链时，应考虑更全面地评估代谢编码的定量潜力，以及将该技术与定量方法（如 qPCR）相结合的可能性。

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引用次数: 0

Advanced evaluation of strawberry quality, consumer preference, and cultivar discrimination through spectral imaging and neural networks 利用光谱成像和神经网络对草莓品质、消费者偏好和品种区分进行高级评价

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-05 DOI: 10.1016/j.foodcont.2025.111339

Salvador Castillo-Girones , Jos Ruizendaal , Xiomara Salas-Valderrama , Sandra Munera , Jose Blasco , Gerrit Polder

Strawberries are among the most popular fruits, and meeting the rising demand for high-quality, flavorful varieties requires understanding consumer preferences. Accurately predicting these preferences, assessing quality, and preventing food fraud are crucial for breeders and sellers. This helps breeders develop superior cultivars and enables sellers to sort and market strawberries by taste and quality. This study explores the prediction of the quality and the acceptance of Dutch consumers of seventeen strawberry cultivars and their discrimination using VIS-NIR spectral imaging with a spectral range between 400 and 1000 nm and Artificial Neural Networks (ANNs), which was not done before. A total of 3564 samples were utilized. Three algorithms: Support Vector Machine, XGBoost, and a Multilayer Perceptron (MLP), were evaluated to predict quality parameters, consumer acceptance, and cultivar discrimination. MLP models showed the highest accuracy, with R² values of 0.85 for total soluble solids, 0.81 for titratable acidity, 0.76 for bite, and 0.78 for overall consumer acceptance. For cultivar discrimination, the MLP model achieved an F1 score of 0.84. These findings highlight the potential of ANNs in enhancing product quality assessment, preventing food fraud, and aligning products with consumer preferences in the food industry.

草莓是最受欢迎的水果之一，要满足对高品质、美味品种不断增长的需求，就需要了解消费者的偏好。准确预测这些偏好，评估质量和防止食品欺诈对育种者和销售商至关重要。这有助于育种者培育优质品种，并使销售商能够根据口味和质量对草莓进行分类和销售。本研究利用光谱范围在400 ~ 1000 nm的VIS-NIR光谱成像和人工神经网络（ann）技术，对17个荷兰草莓品种的质量和接受度进行了预测，并对其进行了鉴别。总共使用了3564个样本。三种算法：支持向量机、XGBoost和多层感知器（MLP），用于预测质量参数、消费者接受度和品种区分。MLP模型显示出最高的准确性，总可溶性固形物的R2值为0.85，可滴定酸度的R2值为0.81，咬合度的R2值为0.76，总体消费者接受度的R2值为0.78。对于品种识别，MLP模型的F1得分为0.84。这些发现强调了人工神经网络在加强产品质量评估、防止食品欺诈以及使产品符合食品行业消费者偏好方面的潜力。

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引用次数: 0

Aspergillus versicolor as producer of known and novel aflatoxin precursors on hard cheese 硬奶酪中已知和新型黄曲霉毒素前体的制造者

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-04 DOI: 10.1016/j.foodcont.2025.111337

Kim Lara Gützkow , Ronald Maul , Sascha Rohn , Markus Schmidt-Heydt

Although, mycotoxins like sterigmatocystin (STC) and ochratoxin A (OTA) have been identified as regular contaminats in grated grana-type cheeses, limited research has been conducted to determine the responsible fungi. In 7 out of 9 grated grana-type cheese samples STC was detected by HPLC-MS/MS analysis, with two of these also containing OTA. Notably, methoxy-STC was detected for the first time in food matrix, raising concerns due to the toxic and mutagenic effects similar to those of STC, highlighting important implications for food safety. The mycobiome analysis of fungi isolated from cheese confirmed Aspergillus versicolor as the main producer of STC. An inoculation experiment using grated Parmigiano Reggiano and the isolated A. versicolor strain confirmed the production of STC together with some biosynthetic precursors. Furthermore, these results suggest that environmental factors and substrate composition influence mycotoxin biosynthesis and might have an impact beyond genetic predispositions. However, OTA was not detected in the inoculation experiment, suggesting that the presence of additional mycotoxins in grated grana-type cheese originated from further fungal co-contamination.

虽然像sterigmatocystin （STC）和赭曲霉毒素A （OTA）这样的真菌毒素已被确定为磨碎的格兰奶酪中的常规污染物，但对确定负责真菌的研究有限。HPLC-MS/MS分析9份磨碎的格兰奶酪样品中有7份检测到STC，其中2份也含有OTA。值得注意的是，甲氧基-STC首次在食品基质中被检测到，由于其具有与STC相似的毒性和诱变作用，引起了人们的关注，突出了对食品安全的重要意义。奶酪中分离真菌的菌群分析证实了花色曲霉是STC的主要产生菌。用磨碎的帕尔马干酪和分离的花斑A.菌株接种实验证实了STC的产生以及一些生物合成前体。此外，这些结果表明环境因素和底物组成影响霉菌毒素的生物合成，并且可能具有遗传易感性以外的影响。然而，在接种实验中未检测到OTA，这表明磨碎的谷物型奶酪中存在额外的真菌毒素源于进一步的真菌共污染。

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引用次数: 0

Microplastic accumulation in four commercial fish from fish market: Implications for human dietary risk assessment 来自鱼市场的四种商业鱼类的微塑料积累：对人类饮食风险评估的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-04 DOI: 10.1016/j.foodcont.2025.111332

Renkang Jin , Li'ang Li , Shixiu Wang , Menghong Hu , Wei Huang , Youji Wang

Microplastics (MPs) exist widely in aquaculture waters, and are inevitably ingested by farmed aquatic animals, but the subsequent dietary risks of consuming aquaculture products are unclear. We seasonally sampled four commercially important fish species (Aristichthys nobilis, Siniperca chuatsi, Carassius auratus, and Trichiurus lepturus) from Shanghai fish market to analyze the MPs in these fish. An optimized digestion protocol (10 % KOH, 60°C, 24 h) yielded the 95.28 % MPs recovery rate. MPs in commercial fish were quantified and characterized through dissecting microscope and micro-Fourier transform infrared spectrometer (μ-FTIR), respectively (spectral match threshold >70 %; limit of detection: 50 μm). Human dietary exposure risks were assessed by correlating MP abundance with region-specific aquatic product consumption patterns. The results showed a total of 11 types of natural, semi-synthetic and synthetic polymers were detected, with a detection rate of 60.94 %. The most abundant polymer types were polyester (PES), rayon (RY), polyethylene terephthalate (PET), and cellulose (CE). MPs abundance in edible tissues was from 0.000 to 0.029 items/g. Gut accumulated the most MPs, followed by gills, viscera, belly, and head. People annual dietary intake (ADI) of MPs was roughly 241 items/year via fish, with the estimated dietary intake (EDI) reaching 3.45 items/year/kg for males and 4.81 items/year/kg for females. The polymer risk index (H_i) for the quartet of assessed fish species indicated a low to medium risk. Our study emphasizes the dietary risk posed by MPs in typical commercial fish. However, the risk management for MPs still needs to be enhanced in the future.

微塑料（MPs）广泛存在于水产养殖水域，不可避免地被养殖水生动物摄入，但食用水产养殖产品的后续饮食风险尚不清楚。本研究在上海鱼市季节性取样了4种重要的商业鱼类（鳙鱼、翘嘴鳜、鲫鱼和瘦毛鲫），分析了这些鱼类的MPs含量。优化的消解方案（10% KOH, 60°C, 24 h）的MPs回收率为95.28%。分别通过解剖显微镜和微傅里叶变换红外光谱仪（μ-FTIR）对商品鱼中的MPs进行了定量和表征(光谱匹配阈值>； 70%；检测限：50 μm)。通过将MP丰度与区域特定水产品消费模式相关联来评估人类饮食暴露风险。结果表明，共检出天然、半合成和合成聚合物11种，检出率为60.94%。最丰富的聚合物类型是聚酯（PES）、人造丝（RY）、聚对苯二甲酸乙二醇酯（PET）和纤维素（CE）。食用组织中MPs丰度为0.000 ~ 0.029项/g。肠道累积的MPs最多，其次是鳃、内脏、腹部和头部。鱼类对MPs的年膳食摄入量（ADI）约为241项/年，男性的估计膳食摄入量（EDI）为3.45项/年/kg，女性为4.81项/年/kg。四种被评估鱼类的聚合物风险指数（Hi）显示为低至中等风险。我们的研究强调了MPs对典型商业鱼类的饮食风险。然而，未来对国会议员的风险管理仍需加强。

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引用次数: 0

FISH-FIT: A web-based tool to improve European seafood authenticity control FISH-FIT：一个基于网络的工具，用于改善欧洲海鲜的真实性控制

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-04 DOI: 10.1016/j.foodcont.2025.111335

Ute Schröder , Carmen G. Sotelo , Regina Klapper

Seafood is one of foods most prone to substitution and fraud potentially leading to negative implications on consumer trust and health. Additionally, seafood adulteration might be accompanied by overfishing of particular species. Therefore, the European Union issued several regulations on the declaration of seafood and its control. In support of the implementation of these regulations as well as research on seafood authenticity, the user-friendly database called FISH-FIT has been established. FISH-FIT provides users with valid DNA sequences of authentic tissue samples, a sequence alignment tool and a collection of methods relevant to this field. Furthermore, authentic tissue materials are physically stored for all entries in the database and aliquots can be requested directly via the website. The FISH-FIT resource allows users to compare their samples with verified materials and aids in the development of new DNA-based methods for authentication. Overall, FISH-FIT contributes to the harmonisation of seafood authentication methods in Europe in the long term.

海产品是最容易被替代和欺诈的食品之一，可能对消费者的信任和健康产生负面影响。此外，海鲜掺假可能伴随着对特定物种的过度捕捞。因此，欧盟颁布了几项关于海产品申报及其控制的规定。为了支持这些法规的实施以及对海鲜真实性的研究，已经建立了一个名为FISH-FIT的用户友好数据库。FISH-FIT为用户提供真实组织样本的有效DNA序列，序列比对工具和与该领域相关的方法集合。此外，真实的组织材料被物理存储在数据库中的所有条目中，并且可以直接通过网站请求配额。FISH-FIT资源允许用户将他们的样品与经过验证的材料进行比较，并有助于开发新的基于dna的认证方法。总的来说，从长远来看，FISH-FIT有助于欧洲海产品认证方法的统一。

引用次数: 0

Quantification of aflatoxin B1 in wheat using a natural pigment sensing array 利用天然色素传感阵列定量测定小麦黄曲霉毒素B1

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-04-03 DOI: 10.1016/j.foodcont.2025.111336

Hui Jiang , Dengmin Li , Jihong Deng , Quansheng Chen

The accumulation of aflatoxin B1 (AFB1) during wheat storage may pose a potential threat to food safety and quality control. This study explores the application of a colorimetric sensor array based on natural pigments for the quantitative detection of AFB1 and evaluates its detection performance. Anthocyanin dyes were extracted from various plant materials, and nine dyes with excellent response characteristics were selected to construct a sensor array for capturing volatile gas information released by wheat samples with different degrees of mold contamination. Subsequently, the ReliefF algorithm and SVM_Rfe algorithm were used to optimize the color components of the differential images from the sensor array. A back-propagation neural network (BPNN) model was constructed based on the best combination of color features, and the parameters of the network were adjusted using the particle swarm optimization (PSO) algorithm. The results showed that after the optimization of color components, the root mean square error (RMSE) of the BPNN model on the prediction set decreased from 4.4362 μg kg⁻¹ to 3.7699 μg kg⁻¹, while the correlation coefficient (R) increased to 0.9828. In general, the natural pigment-based sensor arrays based on natural pigments combined with chemometric methods can play an important role in grain mycotoxin detection and provide a non-destructive, rapid and environmentally friendly method for quantitative detection of mycotoxins in stored grains. Meanwhile, the feature optimization strategy significantly reduces the complexity and cost of sensor array construction, demonstrating excellent application potential.

小麦储存过程中黄曲霉毒素 B1（AFB1）的积累可能对食品安全和质量控制构成潜在威胁。本研究探索了基于天然色素的比色传感器阵列在定量检测 AFB1 中的应用，并评估了其检测性能。研究人员从多种植物材料中提取了花青素染料，并选择了 9 种具有优异响应特性的染料构建了传感器阵列，用于捕捉不同霉菌污染程度的小麦样品释放的挥发性气体信息。随后，利用 ReliefF 算法和 SVM_Rfe 算法对传感器阵列差分图像的颜色成分进行优化。根据颜色特征的最佳组合构建了反向传播神经网络（BPNN）模型，并使用粒子群优化（PSO）算法调整了网络参数。结果表明，在对颜色成分进行优化后，BPNN 模型在预测集上的均方根误差（RMSE）从 4.4362 μg kg-1 降至 3.7699 μg kg-1，相关系数（R）升至 0.9828。总之，基于天然色素的传感器阵列与化学计量学方法相结合，可在粮食霉菌毒素检测中发挥重要作用，为储粮中霉菌毒素的定量检测提供了一种无损、快速、环保的方法。同时，特征优化策略大大降低了传感器阵列构建的复杂性和成本，显示出良好的应用潜力。

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