Nur Ahlina Damayanti, N. Nusdwinuringtyas, Tresia Fransiska Uliana Tambunan, A. Kekalih
The high-intensity interval training has been seen to be beneficial for various populations. This study aimed to determine the effect of high-intensity interval training (HIIT) on blood lactate levels and rate of perceived exertion (RPE) in sedentary healthy adults. A pre-post study was conducted on adults that were stated healthy and sedentary on physical activity. All participants were given HIIT with a total duration of 20 minutes for 12 sessions using a treadmill. Blood lactate level and RPE was recorded on the 1st and 12th session. A significant decrease in blood lactate level and RPE after giving HIIT was found with a reduction of 1,1 mmol/ L and 2 on the Borg Scale respectively (p<0.0001). Further research is needed using field-based exercise testing to determine exercise prescription and to obtain the benefit of HIIT in populations with cardiovascular risk factors before utilizing it in patients.
{"title":"The Effect of High-Intensity Interval Training on Blood Lactate Levels and Rate of Perceived Exertion in Sedentary Healthy Adults","authors":"Nur Ahlina Damayanti, N. Nusdwinuringtyas, Tresia Fransiska Uliana Tambunan, A. Kekalih","doi":"10.33533/jpm.v16i2.5104","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.5104","url":null,"abstract":"The high-intensity interval training has been seen to be beneficial for various populations. This study aimed to determine the effect of high-intensity interval training (HIIT) on blood lactate levels and rate of perceived exertion (RPE) in sedentary healthy adults. A pre-post study was conducted on adults that were stated healthy and sedentary on physical activity. All participants were given HIIT with a total duration of 20 minutes for 12 sessions using a treadmill. Blood lactate level and RPE was recorded on the 1st and 12th session. A significant decrease in blood lactate level and RPE after giving HIIT was found with a reduction of 1,1 mmol/ L and 2 on the Borg Scale respectively (p<0.0001). Further research is needed using field-based exercise testing to determine exercise prescription and to obtain the benefit of HIIT in populations with cardiovascular risk factors before utilizing it in patients.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42361812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Gamayani, Agnes Christine Yolanda Sihite, Iin Pusparini, N. Lailiyya
Obstructive Sleep Apnea/Hypopnea Syndrome (OSAHS) has commonly occured in Down syndrome children caused by the abnormalities in upper respiratory tract anatomy and obesity. This study aims to identify the correlation between Mallampati score and obesity and the risk of OSAHS in Down syndrome children. This was an analytical descriptive cross-sectional study. This study was conducted in children with Down syndrome from September until November 2017. All parents filled the Indonesian Pediatric Sleep Questionnaire (PSQ), then all of the children were examined for Mallampati score and BMI. The correlation was analyzed by the Pearson Chi-Square model. Thirty-six subjects were included in this study. The number of male subjects were slightly more (61.1%), the mean age of the subjects was 8.42±4,449 years, with 52.8% (19 subjects) having OSAHS. There were 23 subjects (63.9%) who had Mallampati scores of 3 and 4, with 13 subjects (36.1%) were obese. This study concluded a statistically significant correlation between Mallampati score and obesity and the risk of OSAHS in Down syndrome children (p-value 0.001 and 0.029). Mallampati score and obesity had a significant correlation with the risk of OSAHS in Down syndrome children.
{"title":"The Relationship Between Mallampati Score And Obesity With the Risk of OSAHS in Down Syndrome Children","authors":"U. Gamayani, Agnes Christine Yolanda Sihite, Iin Pusparini, N. Lailiyya","doi":"10.33533/jpm.v16i1.3960","DOIUrl":"https://doi.org/10.33533/jpm.v16i1.3960","url":null,"abstract":"Obstructive Sleep Apnea/Hypopnea Syndrome (OSAHS) has commonly occured in Down syndrome children caused by the abnormalities in upper respiratory tract anatomy and obesity. This study aims to identify the correlation between Mallampati score and obesity and the risk of OSAHS in Down syndrome children. This was an analytical descriptive cross-sectional study. This study was conducted in children with Down syndrome from September until November 2017. All parents filled the Indonesian Pediatric Sleep Questionnaire (PSQ), then all of the children were examined for Mallampati score and BMI. The correlation was analyzed by the Pearson Chi-Square model. Thirty-six subjects were included in this study. The number of male subjects were slightly more (61.1%), the mean age of the subjects was 8.42±4,449 years, with 52.8% (19 subjects) having OSAHS. There were 23 subjects (63.9%) who had Mallampati scores of 3 and 4, with 13 subjects (36.1%) were obese. This study concluded a statistically significant correlation between Mallampati score and obesity and the risk of OSAHS in Down syndrome children (p-value 0.001 and 0.029). Mallampati score and obesity had a significant correlation with the risk of OSAHS in Down syndrome children. ","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49268512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Online learning is learning that is done without face-to-face but through available platforms. All forms of learning materials are distributed online. Communication is done online and tests are also conducted online. During the Covid-19 pandemic, distance learning using devices such as laptops and smartphones is very much needed to support distance learning activities. This has an impact on increasing the risks that arise such as back pain.
{"title":"Risk of Back Pain in Faculty of Medicine UPN Veteran Jakarta Students During Online Lectures in the 2020 COVID-19 Pandemic Era","authors":"Dwi Arwandi Yogi Saputra, Hermawan Saputra, Sutoto Sutoto","doi":"10.33533/jpm.v16i2.4815","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4815","url":null,"abstract":"Online learning is learning that is done without face-to-face but through available platforms. All forms of learning materials are distributed online. Communication is done online and tests are also conducted online. During the Covid-19 pandemic, distance learning using devices such as laptops and smartphones is very much needed to support distance learning activities. This has an impact on increasing the risks that arise such as back pain.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45277998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inflammation is caused by infectious diseases, including COVID-19. Severe inflammatory responses contribute to weak adaptive immune response, there by resulting in immune response imbalance. Circulating biomarkers can represent inflammation and immune status are potential predictors for the prognosis of COVID-19 patients. Ferritin and interleukin-6 can serve as significant biomarkers in the detection of cytokine storm, systemic inflammation, and the prognosis of COVID-19. Neutrophil-lymphocyte ratio is an independent prognostic biomarker for COVID-19 patients. The aim of this study to determine differences levels of IL-6, ferritin, and NLR in COVID-19 patients treated ICU and non-ICU. This study was comparative cross-sectional design of 62 COVID-19 patients. Research was conducted at the Central Laboratory and Medical Record of Dr. M. Djamil Padang hospital (May-September 2021). Interleukin-6 levels determined the ECLIA methods, ferritin with the ELFA methods, and NLR with calculation methods. Bivariate data were analyzed with Mann-Whitney test. The characteristics of COVID-19 patients treated in ICU: 64.5% male, average age was 53.52(10.71) years, 48.4% death, and non ICU: female 71.0%, average 42.32 (12.22) years, and recovered 100%. The most common comorbid is hypertension. There were significant differences levels of IL-6, ferritin, and NLR in ICU and non-ICU patients with COVID-19 (p<0.001).
{"title":"Comparison in Levels of Interleukin 6, Ferritine and Neutrophil-Lymphocyte Ratio in COVID-19 Patiens Treated in ICU and Non-ICU","authors":"Nanda Oktavia, Efrida Efrida, Z. Rofinda","doi":"10.33533/jpm.v16i2.4891","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4891","url":null,"abstract":"Inflammation is caused by infectious diseases, including COVID-19. Severe inflammatory responses contribute to weak adaptive immune response, there by resulting in immune response imbalance. Circulating biomarkers can represent inflammation and immune status are potential predictors for the prognosis of COVID-19 patients. Ferritin and interleukin-6 can serve as significant biomarkers in the detection of cytokine storm, systemic inflammation, and the prognosis of COVID-19. Neutrophil-lymphocyte ratio is an independent prognostic biomarker for COVID-19 patients. The aim of this study to determine differences levels of IL-6, ferritin, and NLR in COVID-19 patients treated ICU and non-ICU. This study was comparative cross-sectional design of 62 COVID-19 patients. Research was conducted at the Central Laboratory and Medical Record of Dr. M. Djamil Padang hospital (May-September 2021). Interleukin-6 levels determined the ECLIA methods, ferritin with the ELFA methods, and NLR with calculation methods. Bivariate data were analyzed with Mann-Whitney test. The characteristics of COVID-19 patients treated in ICU: 64.5% male, average age was 53.52(10.71) years, 48.4% death, and non ICU: female 71.0%, average 42.32 (12.22) years, and recovered 100%. The most common comorbid is hypertension. There were significant differences levels of IL-6, ferritin, and NLR in ICU and non-ICU patients with COVID-19 (p<0.001).","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42388502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lintang Cahyaning Ratri, A. Nugrahani, G. Prabowo, J. H. Prajitno
Diabetes is a chronic metabolic disease that can lead to serious consequences that impair one's quality of life, if not adequately controlled. One of the undesirable complications is diabetic foot ulcer. It is estimated globally that every 30 seconds a leg is amputated due to diabetic foot, and thus can lower the quality of life. Recent studies have used a low-cost dye known as methylene blue as an anti-microorganism agent, and this sparks the idea of exploring more of its possible benefits. This literature review aimed to outline the beneficial roles of methylene blue in diabetic foot ulcer treatment. According to the findings, it is said that methylene blue may play a role as an anti-microorganism agent through its contribution in wound healing and invasive surgical prevention such as limb amputation. All the pooled articles showed a promising outcome of MB from the reduction of wound size in a shorter healing period with none adverse effects reported. Hence, methylene blue may have a promising role to be an effective agent in treating diabetic foot ulcer.
{"title":"Methylene Blue: a Potential Novel Treatment in Diabetic Foot Ulcer","authors":"Lintang Cahyaning Ratri, A. Nugrahani, G. Prabowo, J. H. Prajitno","doi":"10.33533/jpm.v16i2.4792","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4792","url":null,"abstract":"Diabetes is a chronic metabolic disease that can lead to serious consequences that impair one's quality of life, if not adequately controlled. One of the undesirable complications is diabetic foot ulcer. It is estimated globally that every 30 seconds a leg is amputated due to diabetic foot, and thus can lower the quality of life. Recent studies have used a low-cost dye known as methylene blue as an anti-microorganism agent, and this sparks the idea of exploring more of its possible benefits. This literature review aimed to outline the beneficial roles of methylene blue in diabetic foot ulcer treatment. According to the findings, it is said that methylene blue may play a role as an anti-microorganism agent through its contribution in wound healing and invasive surgical prevention such as limb amputation. All the pooled articles showed a promising outcome of MB from the reduction of wound size in a shorter healing period with none adverse effects reported. Hence, methylene blue may have a promising role to be an effective agent in treating diabetic foot ulcer.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44374194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sepsis and electrolyte disturbances are frequently experienced among acute exacerbation of chronic obstructive pulmonary disease (AECOPD) patients. It is characterized by organ failure brought on by unbalanced host response to infection. A 63-year-old male presented to the hospital with the complaint of breathlessness with the oxygen saturation of 76%. The leukocyte level increased to 19.000 and the Potassium level decreased to 2.88. The combination of antibiotics, Potassium supplement, oxygen support through NRM of 15 liters per minute and symptomatic treatment were administered to the patient. The NRM was then changed to HFNC due to his worsening condition and successfully decreased oxygen demand. The patient’s condition has made a significant improvement after seven days of hospitalization. Patients with AECOPD are at higher risk of developing sepsis and electrolyte disturbances. Sepsis will change the biological system of the body due to different pathophysiology such as electrolyte imbalance and obviously engaged with the pathogenesis of ensuing regular intensifications. It is important for the clinicians to know the effective management for the patient, so that the critical phase can be passed even at the limited facilities.
{"title":"Critically Ill Patient with Acute Exacerbation of Chronic Obstructive Pulmonary Disease Developing Sepsis and Potassium Disorder: a Case Report in Secondary Hospital Setting","authors":"Regia Anadhia Pinastika, Indah Rahmawati, Tiara Nadya Putrianda","doi":"10.33533/jpm.v16i2.5071","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.5071","url":null,"abstract":"Sepsis and electrolyte disturbances are frequently experienced among acute exacerbation of chronic obstructive pulmonary disease (AECOPD) patients. It is characterized by organ failure brought on by unbalanced host response to infection. A 63-year-old male presented to the hospital with the complaint of breathlessness with the oxygen saturation of 76%. The leukocyte level increased to 19.000 and the Potassium level decreased to 2.88. The combination of antibiotics, Potassium supplement, oxygen support through NRM of 15 liters per minute and symptomatic treatment were administered to the patient. The NRM was then changed to HFNC due to his worsening condition and successfully decreased oxygen demand. The patient’s condition has made a significant improvement after seven days of hospitalization. Patients with AECOPD are at higher risk of developing sepsis and electrolyte disturbances. Sepsis will change the biological system of the body due to different pathophysiology such as electrolyte imbalance and obviously engaged with the pathogenesis of ensuing regular intensifications. It is important for the clinicians to know the effective management for the patient, so that the critical phase can be passed even at the limited facilities. ","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47204250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Urfa Khairatun Hisan, N. Qomariyah, Kristina Elizabeth
People with dementia have impairment to execute daily life activities by presenting as a deterioration of mental processes, such as memory, thinking, reasoning, and judgment. Many participants in dementia research may lack the capacity to provide informed consent. Additional safeguards are needed for dementia research participants’ protection because of their vulnerability. Only after carefully weighing the risks and possible benefits for the participants in the research may it be decided to use vulnerable participants. The intention to prevent harm pushes against the removal of autonomy. This dilemma is the driving force behind this article's narrative review of the capacity to consent problems in dementia research. For this critical narrative review, we conducted a thorough search of Scopus, PubMed, and Wiley Open Library for literature addressing the ethical and legal issues on the capacity to consent of people with dementia. We outline the dilemmas and difficulties that surround them including the related ethical principles, the informed consent process, capacity to consent, and safeguards for the participant in research involving people with dementia.
{"title":"Capacity to Consent of People with Dementia: a Narrative Review from an Ethical Perspective","authors":"Urfa Khairatun Hisan, N. Qomariyah, Kristina Elizabeth","doi":"10.33533/jpm.v16i2.4744","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4744","url":null,"abstract":"People with dementia have impairment to execute daily life activities by presenting as a deterioration of mental processes, such as memory, thinking, reasoning, and judgment. Many participants in dementia research may lack the capacity to provide informed consent. Additional safeguards are needed for dementia research participants’ protection because of their vulnerability. Only after carefully weighing the risks and possible benefits for the participants in the research may it be decided to use vulnerable participants. The intention to prevent harm pushes against the removal of autonomy. This dilemma is the driving force behind this article's narrative review of the capacity to consent problems in dementia research. For this critical narrative review, we conducted a thorough search of Scopus, PubMed, and Wiley Open Library for literature addressing the ethical and legal issues on the capacity to consent of people with dementia. We outline the dilemmas and difficulties that surround them including the related ethical principles, the informed consent process, capacity to consent, and safeguards for the participant in research involving people with dementia.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69572620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rohmania Setiarini, Made Rika Anastasia Pratiwi, Mirzaulin Leonaviri
The elderly are more likely to experience cognitive impairment, especially those who have a vascular risk factor. The purpose of this study was to determine the relationship between vascular risk factor and cognitive impairment in the elderly. Observational research method with cross-sectional design consist of 79 participant in Tresna Werdha Puspa Karma Social Institution, Mataram from October to November 2021. The subjects were measured blood pressure, weight and height to obtain BMI, glucose level, and total cholesterol. Furthermore, each participant underwent a cognitive function test using Moca-INA. Data were analyzed by chi-square and logistic regression. Diabetes mellitus (p =0.09), hypertension (p =0.037), lower education (p =0,01) and hight blood pressure (p =0.036) were associated with poor cognitive function.
{"title":"Vascular Risk Factor and Cognitive Impairment in Elderly","authors":"Rohmania Setiarini, Made Rika Anastasia Pratiwi, Mirzaulin Leonaviri","doi":"10.33533/jpm.v16i2.4601","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4601","url":null,"abstract":"The elderly are more likely to experience cognitive impairment, especially those who have a vascular risk factor. The purpose of this study was to determine the relationship between vascular risk factor and cognitive impairment in the elderly. Observational research method with cross-sectional design consist of 79 participant in Tresna Werdha Puspa Karma Social Institution, Mataram from October to November 2021. The subjects were measured blood pressure, weight and height to obtain BMI, glucose level, and total cholesterol. Furthermore, each participant underwent a cognitive function test using Moca-INA. Data were analyzed by chi-square and logistic regression. Diabetes mellitus (p =0.09), hypertension (p =0.037), lower education (p =0,01) and hight blood pressure (p =0.036) were associated with poor cognitive function.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43719963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Maemun, Aninda Dinar Widiantari, Nur Aliza, M. Pane, T. Y. Setianingsih, Yeni Afrina, Farida Murtiani
Corynebacterium diphteriae is divided based on its ability to produce toxin. Toxigenic C. diphteriae is the type that has capacity to produce toxin and life threatening. This study was descriptive study in Corynebacterium diphteriae found in children less than 18 years old who were administered to National Infection Center Prof. Dr. Sulianti Saroso. We found 36 viable toxigenic C. diphteriae isolates, which 52.8% intermedius strain, 33.3% gravis strain, 8.3% mitis, and 5.6% non-strain. Most of the host (52,8%) were 60-144 months years old. Majority of the host had completed basic vaccination recommended by Indonesian Paediatrician Association while 75% of them hadn’t gotten the supplementary doses. Fever (48.5%) and odynophagia (54.5%) were experienced by toxigenic intermedius strain infection. Snoring (50%) and thick pseudomembran mostly found in gravis strain host while Bullneck sign found in intermedius strain infection. Complications like airway obstruction, chronic kidney disease, and myocarditis were found in host with toxigenic intermedius strain by 66.7%, 100%, and 75%. Death case were also mostly with this strain. Intermedius strain were mostly found in this study. Complications and mortality were also connected to intermedius strain infection.
{"title":"Toxigenic Diphtheriae Profile in Children in 2017-2018","authors":"S. Maemun, Aninda Dinar Widiantari, Nur Aliza, M. Pane, T. Y. Setianingsih, Yeni Afrina, Farida Murtiani","doi":"10.33533/jpm.v16i2.4865","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4865","url":null,"abstract":"Corynebacterium diphteriae is divided based on its ability to produce toxin. Toxigenic C. diphteriae is the type that has capacity to produce toxin and life threatening. This study was descriptive study in Corynebacterium diphteriae found in children less than 18 years old who were administered to National Infection Center Prof. Dr. Sulianti Saroso. We found 36 viable toxigenic C. diphteriae isolates, which 52.8% intermedius strain, 33.3% gravis strain, 8.3% mitis, and 5.6% non-strain. Most of the host (52,8%) were 60-144 months years old. Majority of the host had completed basic vaccination recommended by Indonesian Paediatrician Association while 75% of them hadn’t gotten the supplementary doses. Fever (48.5%) and odynophagia (54.5%) were experienced by toxigenic intermedius strain infection. Snoring (50%) and thick pseudomembran mostly found in gravis strain host while Bullneck sign found in intermedius strain infection. Complications like airway obstruction, chronic kidney disease, and myocarditis were found in host with toxigenic intermedius strain by 66.7%, 100%, and 75%. Death case were also mostly with this strain. Intermedius strain were mostly found in this study. Complications and mortality were also connected to intermedius strain infection.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43861170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Fiolin, I. H. Dilogo, R. Antarianto, Ludwig Andre Pontoh
Exosomes from Mesenchymal Stem Cells (MSC) is currently one of the highlighted research due to its more specific action on the target tissue. Exosome is one of the MSC Extracellular Vesicle (EVs) that acts as a mediator in the cellular communication. However, not so many literatures have succeed in elaborating its isolation and characterization process. This study aimed to explain the method used in exosomes isolation from Adipose-Derived MSC (ASC) and elaborate its characteristics. This is an in-vitro study performed in the Stem Cell and Tissue Engineering (SCTE) Indonesia Medical Education and Research Institute (IMERI) laboratory. Ultracentrifugation method was performed to isolate exosome from ASC. The exosome were characterized based on its particle size, morphology, and CD63 and CD81 expression for its purity. We were able to isolate sterile exosome from ASC by performing differential ultracentrifugation. The mean size of exosome was 88.7 nm ± 40 nm SD and showed expression of CD63 and CD81. Exosome was successfully isolated from ASC using ultracentrifugation method and characterization following the MISEV standard should be implemented in order to meet the therapeutic efficacy and safety issues as a regenerative agent.
{"title":"Isolation and Characterization of Adipose-Derived Mesenchymal Stem Cell Exosomes: an In-Vitro Study","authors":"J. Fiolin, I. H. Dilogo, R. Antarianto, Ludwig Andre Pontoh","doi":"10.33533/jpm.v16i2.4867","DOIUrl":"https://doi.org/10.33533/jpm.v16i2.4867","url":null,"abstract":"Exosomes from Mesenchymal Stem Cells (MSC) is currently one of the highlighted research due to its more specific action on the target tissue. Exosome is one of the MSC Extracellular Vesicle (EVs) that acts as a mediator in the cellular communication. However, not so many literatures have succeed in elaborating its isolation and characterization process. This study aimed to explain the method used in exosomes isolation from Adipose-Derived MSC (ASC) and elaborate its characteristics. This is an in-vitro study performed in the Stem Cell and Tissue Engineering (SCTE) Indonesia Medical Education and Research Institute (IMERI) laboratory. Ultracentrifugation method was performed to isolate exosome from ASC. The exosome were characterized based on its particle size, morphology, and CD63 and CD81 expression for its purity. We were able to isolate sterile exosome from ASC by performing differential ultracentrifugation. The mean size of exosome was 88.7 nm ± 40 nm SD and showed expression of CD63 and CD81. Exosome was successfully isolated from ASC using ultracentrifugation method and characterization following the MISEV standard should be implemented in order to meet the therapeutic efficacy and safety issues as a regenerative agent.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43173068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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