Pub Date : 2023-08-14DOI: 10.18196/mmjkk.v23i2.18421
Devi Rahmawati, D. Ningrum, Ivana Kinanti Kenyori, Rifki Febriansah, Melany Ayu Octavia, Adi Hermawansyah
Cervical cancer results from abnormal cell development in the cervix and is caused by the Human Papillomavirus (HPV). Cancer treatment with chemotherapy drugs has dangerous side effects because it can attack normal cells. Gnetum gnemon L. seeds are known to possess anticancer potential. Therefore, natural ingredients are necessary to develop new cancer drug strategies with minimal side effects. This study aims to determine the anticancer activity of the ethanol fraction of Gnetum gnemon L. Seeds (EFGS) against HeLa cells. The research method used in this study was an identification test to detect compounds from EFGS using the TLC method and the HeLa cell cytotoxic test using the MTT Assay method. The results showed that EFGS contained stilbenoid group compounds based on TLC-Densitometry, with an Rf value of 0.22 cm. The cytotoxic test results on HeLa cells indicated an IC50 value of 784 µg/mL, classifying it as having the potential as a moderate category anticancer agent. Therefore, this study indicates that EFGS can be utilized as a chemopreventive agent.
{"title":"Anticancer Activity of The Ethanol Fraction of Gnetum gnemon L. Seeds on HeLa Cell Lines","authors":"Devi Rahmawati, D. Ningrum, Ivana Kinanti Kenyori, Rifki Febriansah, Melany Ayu Octavia, Adi Hermawansyah","doi":"10.18196/mmjkk.v23i2.18421","DOIUrl":"https://doi.org/10.18196/mmjkk.v23i2.18421","url":null,"abstract":"Cervical cancer results from abnormal cell development in the cervix and is caused by the Human Papillomavirus (HPV). Cancer treatment with chemotherapy drugs has dangerous side effects because it can attack normal cells. Gnetum gnemon L. seeds are known to possess anticancer potential. Therefore, natural ingredients are necessary to develop new cancer drug strategies with minimal side effects. This study aims to determine the anticancer activity of the ethanol fraction of Gnetum gnemon L. Seeds (EFGS) against HeLa cells. The research method used in this study was an identification test to detect compounds from EFGS using the TLC method and the HeLa cell cytotoxic test using the MTT Assay method. The results showed that EFGS contained stilbenoid group compounds based on TLC-Densitometry, with an Rf value of 0.22 cm. The cytotoxic test results on HeLa cells indicated an IC50 value of 784 µg/mL, classifying it as having the potential as a moderate category anticancer agent. Therefore, this study indicates that EFGS can be utilized as a chemopreventive agent.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"269 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75006308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.18196/mmjkk.v23i2.18947
M. Ulfa, Seshy Tinartayu, Dwi Kartika Nur Fitriani, Sweetina Merkusi, Varianidia Veterini
Since the COVID-19 case was discovered, Indonesia has been one of the affected nations and found various COVID-19 infection and immunization risks. Most females are in the menstrual cycle following COVID-19 infection, regardless of the number of days between cycles, blood quantity, or menses duration. This study examines the changes in menstruation post-COVID-19 infection and vaccination. This study used a cross-sectional observational study with an online questionnaire. The 758 female respondents in Yogyakarta were divided into two groups: 532 females who received vaccination and never have been infected with COVID-19 (Group A) and 226 females with a history of being infected with COVID-19 and vaccination (Group B). The data was analyzed using SPSS 26. The results found that most respondents aged 17-25 (90.24%) were students (80.87%). Both two groups had acne on Pre-menstrual syndrome (28.16%), 5-8 days of menstrual duration (73.75%), 30 days of menstruation duration length (57%), and the regular menstruation volume (63.19%) after COVID-19 vaccination. The comparative analysis of menstruation changes in two groups (p0.05), including menstrual duration (0.771), menstrual duration length (0.609), and menstrual volume (0.476) after COVID-19 vaccination. In conclusion, there was no significant difference in menstrual change between Group A and Group B. After vaccination, healthcare professionals can promote post-COVID-19 immunization counseling and services to women who experience an abnormal menstrual cycle.
{"title":"Changes in Women’s Menstruation Cycle After COVID-19 Vaccination and Infection","authors":"M. Ulfa, Seshy Tinartayu, Dwi Kartika Nur Fitriani, Sweetina Merkusi, Varianidia Veterini","doi":"10.18196/mmjkk.v23i2.18947","DOIUrl":"https://doi.org/10.18196/mmjkk.v23i2.18947","url":null,"abstract":"Since the COVID-19 case was discovered, Indonesia has been one of the affected nations and found various COVID-19 infection and immunization risks. Most females are in the menstrual cycle following COVID-19 infection, regardless of the number of days between cycles, blood quantity, or menses duration. This study examines the changes in menstruation post-COVID-19 infection and vaccination. This study used a cross-sectional observational study with an online questionnaire. The 758 female respondents in Yogyakarta were divided into two groups: 532 females who received vaccination and never have been infected with COVID-19 (Group A) and 226 females with a history of being infected with COVID-19 and vaccination (Group B). The data was analyzed using SPSS 26. The results found that most respondents aged 17-25 (90.24%) were students (80.87%). Both two groups had acne on Pre-menstrual syndrome (28.16%), 5-8 days of menstrual duration (73.75%), 30 days of menstruation duration length (57%), and the regular menstruation volume (63.19%) after COVID-19 vaccination. The comparative analysis of menstruation changes in two groups (p0.05), including menstrual duration (0.771), menstrual duration length (0.609), and menstrual volume (0.476) after COVID-19 vaccination. In conclusion, there was no significant difference in menstrual change between Group A and Group B. After vaccination, healthcare professionals can promote post-COVID-19 immunization counseling and services to women who experience an abnormal menstrual cycle.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86259817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-11DOI: 10.18196/mmjkk.v23i2.17848
A. Andromeda, Muhammad Luthfi Rafshanzany, Joseph Dharmadi Buntoro
Malnutrition is a global health problem often found in children and adolescents. This condition can inhibit growth and development in children. Soil transmission helminth (STH) infection absorbs nutrients in the host's digestive system, affecting the host's nutritional status. One of the risk factors for STH infection is poor personal hygiene. This study aims to analyze the association of STH infections and personal hygiene with the nutritional status of elementary school children. This research is a cross-sectional study with primary data conducted in August – September 2022 on 92 children aged 6 – 12 years at Neglasari Elementary School, Cilame Village, District Bandung. Nutritional status was assessed by measuring height and weight to calculate body mass index and then putting it into the WHO 2007 Z-Score plotting curve. STH infections were determined by microscopic examination of helminth eggs from feces using the Kato-Katz method. Personal hygiene was assessed through direct interview methods. The results showed that there was no STH infection in all research respondents. The distribution of nutritional status was dominated by normal nutrition, with 66 (71.7%) respondents and the majority had implemented good personal hygiene behavior habits (78 (84.8%) respondents). It can be concluded that there was a significant relationship between personal hygiene and nutritional status in students (p= 0.000). The average score of children who had implemented good personal hygiene was in normal nutritional status.
{"title":"Association Between Personal Hygiene and Nutritional Status in School-Age Children","authors":"A. Andromeda, Muhammad Luthfi Rafshanzany, Joseph Dharmadi Buntoro","doi":"10.18196/mmjkk.v23i2.17848","DOIUrl":"https://doi.org/10.18196/mmjkk.v23i2.17848","url":null,"abstract":"Malnutrition is a global health problem often found in children and adolescents. This condition can inhibit growth and development in children. Soil transmission helminth (STH) infection absorbs nutrients in the host's digestive system, affecting the host's nutritional status. One of the risk factors for STH infection is poor personal hygiene. This study aims to analyze the association of STH infections and personal hygiene with the nutritional status of elementary school children. This research is a cross-sectional study with primary data conducted in August – September 2022 on 92 children aged 6 – 12 years at Neglasari Elementary School, Cilame Village, District Bandung. Nutritional status was assessed by measuring height and weight to calculate body mass index and then putting it into the WHO 2007 Z-Score plotting curve. STH infections were determined by microscopic examination of helminth eggs from feces using the Kato-Katz method. Personal hygiene was assessed through direct interview methods. The results showed that there was no STH infection in all research respondents. The distribution of nutritional status was dominated by normal nutrition, with 66 (71.7%) respondents and the majority had implemented good personal hygiene behavior habits (78 (84.8%) respondents). It can be concluded that there was a significant relationship between personal hygiene and nutritional status in students (p= 0.000). The average score of children who had implemented good personal hygiene was in normal nutritional status.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82513235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Physical activity and eating patterns affect human health. Lack of physical activity and wrong eating patterns will increase BMI, leading to obesity and other diseases. The study aims to determine the relationship between physical activity and eating patterns toward body mass index (BMI) in nursing students. The design of the study was an observational cross-sectional study. The selection of a total of 98 respondents used the technique of a simple random sample collected with the International Physical Activity Questionnaire (IPAQ) for physical activity, eating patterns questionnaire, and measurement of BMI with digital weight scales and height meters. The statistical test used was Spearman's rank correlation. The results of this study indicated a relationship between physical activity, eating patterns and BMI, with a p-value of 0.004 and 0.003, respectively. To conclude, students had a significant relationship between physical activity and eating patterns on BMI. The nursing curriculum should emphasize the importance and benefits of physical activity and eating habits.
{"title":"Relationship between Physical Activity and Eating Patterns Toward Body Mass Index (BMI) in Nursing Students","authors":"Syahruramdhani Syahruramdhani, Rahmi Pratiwi Munarji","doi":"10.18196/mmjkk.v23i2.16809","DOIUrl":"https://doi.org/10.18196/mmjkk.v23i2.16809","url":null,"abstract":"Physical activity and eating patterns affect human health. Lack of physical activity and wrong eating patterns will increase BMI, leading to obesity and other diseases. The study aims to determine the relationship between physical activity and eating patterns toward body mass index (BMI) in nursing students. The design of the study was an observational cross-sectional study. The selection of a total of 98 respondents used the technique of a simple random sample collected with the International Physical Activity Questionnaire (IPAQ) for physical activity, eating patterns questionnaire, and measurement of BMI with digital weight scales and height meters. The statistical test used was Spearman's rank correlation. The results of this study indicated a relationship between physical activity, eating patterns and BMI, with a p-value of 0.004 and 0.003, respectively. To conclude, students had a significant relationship between physical activity and eating patterns on BMI. The nursing curriculum should emphasize the importance and benefits of physical activity and eating habits.","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77607643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.35990/mk.v6n2.p113-122
Rizka Indah Ramadina, P. Utami, Djatiwidodo Edi Pratiknya
Malaria merupakan penyakit menular yang disebabkan oleh parasit apicomplexa darah yaitu Plasmodium sp. khususnya Plasmodium falciparum yang dibawa oleh nyamuk Anopheles. Teripang keling (Holothuria atra) merupakan invertebrata yang mengandung komponen aktif seperti saponin, terpenoid, flavonoid dan alkaloid yang memiliki efektivitas sebagai antiplasmodium. Tujuan dari penelitian ini adalah untuk menganalisis efektivitas antiplasmodial dari ekstrak etanol teripang keling (Holothuria atra) terhadap perkembangan P.falciparum melalui studi in vitro. Dalam studi in vitro penelitian ini menggunakan sampel kultur P.falciparum strain 3D7 dan ekstrak etanol H.atra. Media kultur akan terbagi menjadi 3 kelompok yakni kontrol negatif, kontrol positif serta dengan penambahan ekstrak etanol H.atra. Ketiga kelompok tersebut akan diinkubasi selama 48 jam pada suhu 37oC lalu dilakukan pemeriksaan kadar parasitemia dan inhibitory rate menggunakan mikroskop cahaya serta pengukuran IC50 menggunakan analisis probit melalui program SPSS versi 23. Hasil penelitian memperlihatkan adanya efek antiplasmodial dalam menghambat perkembangan P.falciparum. Semakin besar dosis yang diberikan maka efek hambatannya juga semakin besar. Nilai IC50 ekstrak etanol H.atra adalah 1,54 µg/ml. Penggunaan ekstrak teripang keling (Holothuria atra) memiliki efek antiplasmodial yang mampu menekan perkembangan P.falciparum dan nilai IC50 ekstrak etanol mempunyai aktivitas yang tinggi sebagai antiplasmodial. Kata Kunci: antiplasmodial, Holothuria atra, in vitro, malaria, Plasmodium falciparum
{"title":"STUDI IN VITRO: EFEK ANTIPLASMODIAL EKSTRAK ETANOL \u0000TERIPANG KELING (Holothuria atra) TERHADAP Plasmodium falciparum","authors":"Rizka Indah Ramadina, P. Utami, Djatiwidodo Edi Pratiknya","doi":"10.35990/mk.v6n2.p113-122","DOIUrl":"https://doi.org/10.35990/mk.v6n2.p113-122","url":null,"abstract":"Malaria merupakan penyakit menular yang disebabkan oleh parasit apicomplexa darah yaitu Plasmodium sp. khususnya Plasmodium falciparum yang dibawa oleh nyamuk Anopheles. Teripang keling (Holothuria atra) merupakan invertebrata yang mengandung komponen aktif seperti saponin, terpenoid, flavonoid dan alkaloid yang memiliki efektivitas sebagai antiplasmodium. Tujuan dari penelitian ini adalah untuk menganalisis efektivitas antiplasmodial dari ekstrak etanol teripang keling (Holothuria atra) terhadap perkembangan P.falciparum melalui studi in vitro. Dalam studi in vitro penelitian ini menggunakan sampel kultur P.falciparum strain 3D7 dan ekstrak etanol H.atra. Media kultur akan terbagi menjadi 3 kelompok yakni kontrol negatif, kontrol positif serta dengan penambahan ekstrak etanol H.atra. Ketiga kelompok tersebut akan diinkubasi selama 48 jam pada suhu 37oC lalu dilakukan pemeriksaan kadar parasitemia dan inhibitory rate menggunakan mikroskop cahaya serta pengukuran IC50 menggunakan analisis probit melalui program SPSS versi 23. Hasil penelitian memperlihatkan adanya efek antiplasmodial dalam menghambat perkembangan P.falciparum. Semakin besar dosis yang diberikan maka efek hambatannya juga semakin besar. Nilai IC50 ekstrak etanol H.atra adalah 1,54 µg/ml. Penggunaan ekstrak teripang keling (Holothuria atra) memiliki efek antiplasmodial yang mampu menekan perkembangan P.falciparum dan nilai IC50 ekstrak etanol mempunyai aktivitas yang tinggi sebagai antiplasmodial.\u0000Kata Kunci: antiplasmodial, Holothuria atra, in vitro, malaria, Plasmodium falciparum","PeriodicalId":33234,"journal":{"name":"Jurnal Profesi Medika","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73338022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}