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Contamination dynamics of personal protective equipment (PPE) by SARS-CoV-2 RNA in a makeshift hospital with COVID-19 positive occupants 某方舱医院SARS-CoV-2 RNA对个人防护装备的污染动态
Q2 Medicine Pub Date : 2023-09-09 DOI: 10.1016/j.infpip.2023.100309
Tingting Xia , Shi Shi , Jinyan Yang , Dan Sun , Jijiang Suo , Huihui Kuang , Nana Sun , Hongyan Hu , Jinhan Xiao , Zhongqiang Yan

Background

Personal protective equipment (PPE) helps protect healthcare workers (HCWs) from infection and prevents cross-contamination. Knowledge of the contamination dynamics of PPE during the management of COVID-19 patients in a makeshift hospital is limited.

Aim

To describe the rate of SARS-CoV-2 contamination in PPE and to assess the change of contamination at different time points.

Methods

HCWs were followed up for up to 4 hours with hourly collection of swab samples from PPE surfaces in a makeshift COVID-19 hospital setting. Swabs were tested using quantitative reverse transcription polymerase chain reaction (RT-qPCR) for SARS-CoV-2 RNA.

Results

SARS-CoV-2 was detected on 50.9% of the 1620 swabbed samples from 9 different sites of full-body PPE worn by HCWs. The proportion of sites contaminated with SARS-CoV-2 RNA varied from 10.6% to 95.6%. Viral RNA was most frequently detected from the sole of the outer foot cover (95.6%) and least frequently on the face shield (10.6%). The median Ct values among positive samples were 34.20 (IQR, 32.61–35.22) and 34.05 (IQR, 32.20–35.39) for ORF1ab and N genes, respectively. The highest rate of contamination with SARS-CoV-2 RNA for the PPE swab samples was found after 3 hours of use. The positive rate of outer surface of HEPA filters from air supply device was 82.1% during the full capacity period of the makeshift hospital.

Conclusion

A higher rate of contamination was identified at 3 hours after the entrance to the COVID-19 patient care area. Virus-containing aerosols were trapped in the HEPA filter of air supply equipment, representing a potential protective factor against infection to HCWs.

背景个人防护装备(PPE)有助于保护医护人员免受感染并防止交叉污染。在临时医院管理新冠肺炎患者期间,对PPE污染动态的了解有限。目的描述严重急性呼吸系统综合征冠状病毒2型在个人防护装备中的污染率,并评估不同时间点的污染变化。方法对HCW进行长达4小时的随访,每小时从新冠肺炎临时医院的PPE表面采集拭子样本。使用定量逆转录聚合酶链式反应(RT-qPCR)检测拭子中的严重急性呼吸系统综合征冠状病毒2型RNA。结果,在来自医务人员穿戴的全身PPE 9个不同部位的1620份拭子样本中,50.9%的样本检测到严重急性呼吸系统冠状病毒2型。被严重急性呼吸系统综合征冠状病毒2型核糖核酸污染的部位比例从10.6%到95.6%不等。病毒核糖核酸最常见于外脚蹬(95.6%),最不常见于面罩(10.6%)。ORF1ab和N基因的阳性样本Ct中值分别为34.20(IQR,32.61-35.22)和34.05(IQR),32.20-35.39。PPE拭子样本的严重急性呼吸系统综合征冠状病毒2型核糖核酸污染率最高的是在使用3小时后。在临时医院满负荷运转期间,来自供气装置的高效空气过滤器外表面的阳性率为82.1%。结论进入新冠肺炎患者护理区后3小时发现较高的污染率。含有病毒的气溶胶被困在空气供应设备的高效空气过滤器中,这是一个潜在的保护因素,可以防止感染HCW。
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引用次数: 0
Clinical, microbiological characteristics and predictors of mortality in patients with carbapenemase-producing Enterobacterales bloodstream infections: a multicentre study 产碳青霉烯酶肠杆菌血流感染患者的临床、微生物学特征和死亡率预测因素:一项多中心研究
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100298
Vanesa Anton-Vazquez , Terry John Evans , Samitha Fernando , Donald Somasunderam , Kate David , Mark Melzer , Lois Hawkins , Stephen Morris-Jones , Mauricio Arias , Borana Drazho , Martino Dall’Antonia , Timothy Planche

Objectives

To investigate the clinical, microbiological characteristics and outcomes of patients with bloodstream infections (BSI) due to carbapenemase-producing Enterobacterales (CPE).

Methods

A multicentre retrospective observational study of patients with BSIs due to CPE admitted to six UK hospitals was conducted between 2011 and 2021. Multivariate analysis was used to identify factors predicting 30-day case fatality rate (CFR).

Results

There were 84 episodes of CPE-BSIs, 37 (44%) due to OXA-48, 35 (42%) to metallo-betalactamases (MBL) and 12 (14%) to KPC. 63% of patients were male with a median age of 64 years. Common organisms included Klebsiella spp. (61%), Escherichia coli (20%) and Enterobacter spp. (13%). Urinary devices were more often involved in OXA-48 BSIs (12/37; 32%) compared to infections caused by MBL and KPC (4/35; 11% and 1/12; 8%; P = 0.046). In contrast, central venous catheters were more frequently present in KPC-BSIs (10/12; 92%) compared with OXA-48 and MBL (11/37; 30% and 20/35; 57%; P = 0.002). Effective definitive antimicrobials were received by 72/84 (86%) patients, comprising monotherapy (32/72; 44%) or combination therapy (40/72; 56%). 30-day case fatality rate (CFR) was 38%. Sepsis or septic shock was associated with death [OR 3.81 (CI 1.19–12.14), P = 0.024].

Conclusion

Strategies targeting high-risk patients and adherence to infection prevention bundles for urinary devices and central venous catheters can reduce OXA-48 and KPC-BSIs. Early recognition and management of severe sepsis, prompt initiation of appropriate antimicrobial therapy and development of novel antimicrobials are crucial to mitigate the high CFR associated with CPE-BSIs.

目的探讨产碳青霉烯酶肠杆菌(CPE)血流感染(BSI)患者的临床、微生物学特征及转归。方法对2011年至2021年间在英国6家医院就诊的CPE所致脑损伤患者进行多中心回顾性观察研究。采用多变量分析确定预测30天病死率(CFR)的因素。结果cpe - bsi 84例,OXA-48 37例(44%),金属β -内酰胺酶(MBL) 35例(42%),KPC 12例(14%)。63%的患者为男性,中位年龄64岁。常见病原菌包括克雷伯氏菌(61%)、大肠杆菌(20%)和肠杆菌(13%)。OXA-48 bsi患者更常涉及泌尿系统装置(12/37;32%)与MBL和KPC引起的感染相比(4/35;11%和1/12;8%;P = 0.046)。相比之下,中心静脉导管在kpc - bsi中更常见(10/12;92%),与OXA-48和MBL相比(11/37;30%和20/35;57%;P = 0.002)。72/84(86%)患者接受了有效的决定性抗菌素治疗,包括单药治疗(32/72;44%)或联合治疗(40/72;56%)。30天病死率(CFR)为38%。脓毒症或脓毒性休克与死亡相关[or 3.81 (CI 1.19-12.14), P = 0.024]。结论针对高危患者采取预防感染措施,坚持使用尿路装置和中心静脉置管,可降低OXA-48和kpc - bsi。早期识别和管理严重败血症,及时开始适当的抗菌治疗和开发新型抗菌药物对于减轻cpe - bsi相关的高CFR至关重要。
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引用次数: 0
Effectiveness of supervised implementation of an oral health care protocol on ventilator-associated pneumonia patients in intensive care units: a double-blind multicenter randomized controlled trial 重症监护病房呼吸机相关肺炎患者口腔卫生保健方案监督实施的有效性:一项双盲多中心随机对照试验
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100295
Sharare Karimi , Ensi Kolyaei , Pooya Karimi , Khaled Rahmani

Background

The available scientific evidence suggests a significant association between oral bacteria and the incidence of ventilator-associated pneumonia (VAP).

Aims

The aim of this study was to determine the effectiveness of an oral health protocol in the prevention of ventilator-associated pneumonia.

Methods

In this multi-center RCT, conducted in the intensive care units of neurological patients in three general teaching hospitals of two provinces located in the west of the Iran, a consecutive sample of 200 intubated patients were initially recruited. Patients were randomly allocated to the intervention (received oral health care based on evidence-based oral health protocol) or control (routine oral health care) groups. Both groups received their prescribed treatment regimen for seven consecutive days. The percentage of VAP diagnoses as main outcome assessed using clinical pulmonary infection score (CPIS).

Findings

The rate of VAP in the intervention and control groups was 5% and 64%, respectively. The intervention reduced the risk of VAP by 97% and this difference was statistically significant (P <0.001). The chance of VAP occurrence in patients with lower levels of consciousness in univariate and multivariate analysis was significantly higher (OR: 2.38; 95%CI: 1.11–5.26) P <0.05).

Conclusion

The results of our study suggest that the use of a dynamic supervised oral health care guideline is more effective than the routinely used protocols in the intensive care units of hospitals.

现有的科学证据表明口腔细菌与呼吸机相关性肺炎(VAP)的发病率之间存在显著关联。目的本研究的目的是确定口腔健康方案在预防呼吸机相关性肺炎中的有效性。方法本多中心随机对照试验在伊朗西部两省三家综合教学医院的神经内科重症监护室进行,初始招募连续200例插管患者。患者被随机分配到干预组(根据循证口腔卫生方案接受口腔卫生保健)或对照组(常规口腔卫生保健)组。两组均连续7天按照规定的治疗方案进行治疗。使用临床肺部感染评分(CPIS)评估VAP诊断为主要结局的百分比。结果干预组和对照组VAP发生率分别为5%和64%。干预使VAP的风险降低了97%,这一差异具有统计学意义(P <0.001)。在单因素和多因素分析中,意识水平较低的患者发生VAP的几率显著较高(OR: 2.38;95%CI: 1.11-5.26) P <0.05)。结论在医院重症监护病房使用动态监督口腔保健指南比常规方案更有效。
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引用次数: 0
Operating room disinfection: operator-driven ultraviolet ‘C’ vs. chemical treatment 手术室消毒:操作员驱动的紫外线“C”与化学处理
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100301
Marie-Claire Fickenscher , Madeline Stewart , Ryan Helber , Edward J. Quilligan , Arthur Kreitenberg , Carlos A. Prietto , Vance O. Gardner

Background

In operating room (OR) surfaces, Nosocomial pathogens can persist on inanimate surfaces for long intervals and are highly resistant to traditional surface cleaning.

Aim

This study compares traditional chemical operating room terminal disinfection to a unique operator-driven device that emits germicidal UV light at short distance onto vertical and horizontal surfaces.

Methods

A randomized crossover analogous protocol assigned 40 end-of-day operating rooms into either group A (chemical then UVC treatments) or group B (UVC then chemical treatments). Initial Staphylococcal cultures were obtained prior to disinfection treatment, after the first treatment, and after the second treatment at 16 most commonly contaminated sites to represent overall room contamination. Success was defined as no growth and failure as 1 or more colony forming units. Thoroughness of chemical treatment vs UVC treatment was compared and used to determine if the second treatment was additive to the first treatment within each group.

Findings

The operator driven UVC device outperformed chemical treatment in reducing the number of contaminated sites in the OR by more than half (P<0.001). Operator-driven UVC reduced contaminated sites after chemical treatment by nearly half (P<0.001). In contrast, chemical treatment after operator-driven UVC did not significantly reduce the number of contaminated sites. The mean employee time of disinfection for chemical treatment was 49 minutes and for the operator-driven UVC emitter 7.9 minutes (P<0.001).

Conclusions

This study demonstrates that addition of an operator-driven UVC emitter to OR rooms between cases could be helpful in overall decreasing the number of contaminated sites.

在手术室(OR)的表面,医院病原菌可以在无生命的表面持续很长时间,并且对传统的表面清洁具有很强的抵抗力。目的将传统的化学手术室终末消毒与一种独特的操作者驱动的向垂直和水平表面发射近距离杀菌紫外线的装置进行比较。方法采用随机交叉模拟方案,将40间日间手术室分为A组(化学+ UVC处理)和B组(UVC +化学处理)。在消毒处理前、第一次处理后和第二次处理后,分别在16个最常被污染的地点进行了初始葡萄球菌培养,以代表整个房间的污染情况。成功定义为没有生长,失败定义为1个或更多的菌落形成单位。比较化学处理和UVC处理的彻底性,并用于确定每组中第二次处理是否为第一次处理的附加性。研究结果:操作员驱动的UVC设备在减少手术室污染部位数量方面优于化学处理,减少了一半以上(P<0.001)。操作员驱动的UVC使化学处理后的污染区域减少了近一半(P<0.001)。相比之下,在操作员驱动的UVC之后进行化学处理并没有显著减少污染场地的数量。化学处理的平均消毒时间为49分钟,操作人员驱动的UVC发射器的平均消毒时间为7.9分钟(P<0.001)。本研究表明,在病例之间的手术室中增加操作员驱动的UVC发射器可能有助于总体减少污染场所的数量。
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引用次数: 0
Inactivation kinetics of benzalkonium chloride and ethanol-based hand sanitizers against a betacoronavirus and an alphacoronavirus 苯扎氯铵和乙醇基洗手液对乙型冠状病毒和甲型冠状病毒的失活动力学
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100293
Brandon L. Herdt , Luisa A. Ikner

Background

Hand hygiene is critical to lower the potential for the spread of SARS-CoV-2 and other infectious agents by direct contact. When running water and soap are not available for hand hygiene, ethanol-based hand sanitizers are currently the recommended standard of care [1–3]. Though recently published data showed comparable in vitro effectiveness of benzalkonium chloride (BAK)-based and ethanol-based hand sanitizers against SARS-CoV-2 virus, a paucity of peer-reviewed data on the effectiveness of these formulations against other types of infective coronaviruses remains. This work assessed human coronavirus HCoV-229E (genus Alphacoronavirus) concurrently with SARS-CoV-2, Isolate USA-WA1/2020 (genus Betacoronavirus) to fill this gap.

Methods

The test was conducted according to EN14476:2013-A2:2019 [EN14476] Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area [4]. Two BAK-based hand sanitizers, five ethanol-based hand sanitizers, and an 80% ethanol reference formulation were tested for antiviral activity against SARS-CoV-2 and HCoV-229E at 15- and 30- second contact times.

Results

Both SARS-CoV-2 and HCoV-229E were reduced by greater than 4.00-log10 within 15 seconds of contact. Virus decay constants (k) following first-order kinetics were similar for BAK and ethanol-based formulations against both test viruses. The SARS-CoV-2 results reported herein mirrored previous data reported by Herdt et al. (2021).

Conclusion

BAK and ethanol hand sanitizer formulations inactivate SARS-CoV-2 and HCoV-229E at similar rates. This data supports previously published effectiveness data for both chemistries and indicates that additional coronavirus strains and variants would demonstrate similar inactivation trends.

手部卫生对于降低SARS-CoV-2和其他感染原通过直接接触传播的可能性至关重要。当没有自来水和肥皂用于手部卫生时,目前推荐使用含乙醇的洗手液作为护理标准[1-3]。尽管最近发表的数据显示,基于苯扎氯铵(BAK)的洗手液和基于乙醇的洗手液对SARS-CoV-2病毒的体外有效性相当,但关于这些配方对其他类型传染性冠状病毒的有效性的同行评审数据仍然缺乏。本研究对人类冠状病毒HCoV-229E(甲型冠状病毒属)与SARS-CoV-2同时进行了评估,分离出USA-WA1/2020(乙型冠状病毒属)来填补这一空白。方法按照EN14476:2013- a2:2019 [EN14476]《医用区域毒力定量悬浮试验评价》进行试验。在15秒和30秒的接触时间下,测试了两种基于bakv的洗手液、五种基于乙醇的洗手液和80%乙醇的参考配方对SARS-CoV-2和HCoV-229E的抗病毒活性。结果SARS-CoV-2和HCoV-229E在接触15秒内均降低了4.00-log10以上。基于BAK和乙醇的配方对两种测试病毒的一阶动力学下的病毒衰变常数(k)相似。本文报告的SARS-CoV-2结果反映了Herdt等人(2021)先前报告的数据。结论bak和乙醇洗手液配方对SARS-CoV-2和HCoV-229E的灭活率相似。这一数据支持了之前发表的两种化学物质的有效性数据,并表明其他冠状病毒毒株和变体将表现出类似的失活趋势。
{"title":"Inactivation kinetics of benzalkonium chloride and ethanol-based hand sanitizers against a betacoronavirus and an alphacoronavirus","authors":"Brandon L. Herdt ,&nbsp;Luisa A. Ikner","doi":"10.1016/j.infpip.2023.100293","DOIUrl":"10.1016/j.infpip.2023.100293","url":null,"abstract":"<div><h3>Background</h3><p>Hand hygiene is critical to lower the potential for the spread of SARS-CoV-2 and other infectious agents by direct contact. When running water and soap are not available for hand hygiene, ethanol-based hand sanitizers are currently the recommended standard of care [1–3]. Though recently published data showed comparable <em>in vitro</em> effectiveness of benzalkonium chloride (BAK)-based and ethanol-based hand sanitizers against SARS-CoV-2 virus, a paucity of peer-reviewed data on the effectiveness of these formulations against other types of infective coronaviruses remains. This work assessed human coronavirus HCoV-229E (genus <em>Alphacoronavirus</em>) concurrently with SARS-CoV-2, Isolate USA-WA1/2020 (genus <em>Betacoronavirus</em>) to fill this gap.</p></div><div><h3>Methods</h3><p>The test was conducted according to EN14476:2013-A2:2019 [EN14476] Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area [4]. Two BAK-based hand sanitizers, five ethanol-based hand sanitizers, and an 80% ethanol reference formulation were tested for antiviral activity against SARS-CoV-2 and HCoV-229E at 15- and 30- second contact times.</p></div><div><h3>Results</h3><p>Both SARS-CoV-2 and HCoV-229E were reduced by greater than 4.00-log<sub>10</sub> within 15 seconds of contact. Virus decay constants (<em>k</em>) following first-order kinetics were similar for BAK and ethanol-based formulations against both test viruses. The SARS-CoV-2 results reported herein mirrored previous data reported by Herdt <em>et al.</em> (2021).</p></div><div><h3>Conclusion</h3><p>BAK and ethanol hand sanitizer formulations inactivate SARS-CoV-2 and HCoV-229E at similar rates. This data supports previously published effectiveness data for both chemistries and indicates that additional coronavirus strains and variants would demonstrate similar inactivation trends.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/e4/main.PMC10266983.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9738855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of the COVID-19 pandemic on the incidence of healthcare-associated Clostridioides difficile infection in a tertiary healthcare facility in the Republic of Ireland 2019冠状病毒病大流行对爱尔兰共和国三级卫生保健机构中与卫生保健相关的艰难梭菌感染发生率的影响
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100300
Saied Ali, Sinead McDermott

Background

Clostridioides difficile is the foremost cause of nosocomial infectious diarrhoea and one of the most prevalent healthcare associated infections (HAIs).

Aims

To investigate the impact of the coronavirus disease 2019 (COVID-19) pandemic on the incidence of healthcare associated C. difficile infection (HA-CDI).

Methods

A retrospective study was conducted from January 2019–December 2022 inclusive at a tertiary University Hospital in Dublin, Ireland. The study period was divided into COVID-19 and non-COVID-19 periods determined in tangent with the then national incidences of COVID-19 and number of hospitalized patients with COVID-19. Analyses looked at quantity of testing performed, incidence rates and antimicrobial consumption. An independent samples t-test was used to determine significance between groups.

Results

Between COVID-19 and non-COVID-19 periods, no statistically significant difference was observed among HA-CDI rates per 10,000 bed-days (2.1 cases vs 1.76 cases; P=0.34), consumption of defined daily doses per 100 bed-days of antimicrobials – all antimicrobials (83.36 vs 89.5; P=0.091), fluoroquinolones only (3.71 vs 4.46; P=0.067), third-generation cephalosporins only (4.17 vs 4.43; P=0.449), carbapenems only (3.28 vs 3.26; P=0.944) – or the number of C. difficile tests performed per 10,000 bed-days (321.81 tests vs 326.63 tests; P=0.696).

Conclusions

There was no difference in the incidence rates of HA-CDI between COVID-19 and non-COVID-19 periods at our institution.

背景艰难梭菌是医院感染性腹泻的主要原因,也是最常见的卫生保健相关感染之一。目的探讨2019冠状病毒病(COVID-19)大流行对卫生保健相关性艰难梭菌感染(HA-CDI)发生率的影响。方法回顾性研究于2019年1月至2022年12月在爱尔兰都柏林的一家三级大学医院进行。根据当时全国COVID-19发病率和住院患者人数,将研究期分为COVID-19期和非COVID-19期。分析着眼于所进行的检测数量、发病率和抗微生物药物消耗量。采用独立样本t检验确定组间显著性。结果在COVID-19与非COVID-19期间,HA-CDI发生率(每万床日2.1例vs 1.76例;P=0.34),每100个住院日使用规定每日剂量的抗菌素-所有抗菌素(83.36 vs 89.5;P=0.091),仅使用氟喹诺酮类药物(3.71 vs 4.46;P=0.067),仅使用第三代头孢菌素(4.17 vs 4.43;P=0.449),仅碳青霉烯类(3.28 vs 3.26;P=0.944)——或每10,000个床位日进行艰难梭菌测试的次数(321.81次测试对326.63次测试;P = 0.696)。结论我院新冠肺炎期间HA-CDI与非新冠肺炎期间HA-CDI发生率无差异。
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引用次数: 0
Risk of COVID-19 in different groups of healthcare professionals between February 2020 and June 2021 in Finland: a register-based cohort study 芬兰2020年2月至2021年6月期间不同医疗保健专业人员群体中COVID-19的风险:一项基于登记册的队列研究
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100297
Sohvi Kääriäinen , Ulla Harjunmaa , Tuula Hannila-Handelberg , Jukka Ollgren , Outi Lyytikäinen

Background

During the Coronavirus Disease 2019 (COVID-19) pandemic, healthcare workers (HCWs) have been a risk group for COVID-19.

Aim

To assess the cumulative incidence in different groups of HCWs and the risk factors and outcomes of COVID-19 in HCWs between February 2020 and June 2021 in Finland.

Methods

We linked two national registers, National Infectious Diseases Register (NIDR) and Register of Social Welfare and Healthcare Professionals (Terhikki), using national identity codes. COVID-19 cases were identified from NIDR notifications made by laboratories and physicians, and their healthcare professions from Terhikki. We categorized healthcare professions into seven groups and calculated cumulative incidences using Kaplan-Meier estimate during three periods (1/2/2020−30/6/2020, 1/7/2020−31/12/2020, 1/1/2021−30/6/2021). We identified risk factors in a multivariable model using Cox's regression.

Findings

We identified 8,009 COVID-19-cases among HCWs, with cumulative incidence of 1.79%; 83% were female, median age was 40.9 years (interquartile range, 31.2−51.6). Most COVID-19-cases occurred in nursing assistants (53%) and nurses (17%), with the highest cumulative incidences 2.07% (95%CI, 2.01−2.13%) and 1.82% (95%CI, 1.73−1.91%), respectively. Risk factors were male sex (hazard ratio (HR) 1.2; 95%CI, 1.1−1.3), foreign native language (HR 2.5; 95%CI, 2.2−2.9) and foreign country of birth (HR 1.2; 95%CI, 1.1−1.4). Physician notification data was available for 6,113/8,009 cases (76.3%); 244/6,113 (4.0%) were hospitalized and 37/6,113 (0.6%) in intensive care.

Conclusion

Nurses and nursing assistant, especially men and professionals with foreign background, were at higher risk of COVID-19. This should be specifically addressed during training and implementing infection control measures to protect themselves and patients.

在2019冠状病毒病(COVID-19)大流行期间,卫生保健工作者(HCWs)一直是COVID-19的风险群体。目的评估2020年2月至2021年6月芬兰不同群体卫生保健工作者COVID-19的累积发病率、危险因素和结局。方法我们使用国家身份码将两个国家登记册,即国家传染病登记册(NIDR)和社会福利和卫生保健专业人员登记册(Terhikki)联系起来。从实验室和医生及其来自Terhikki的卫生保健专业人员通报的NIDR中确定了COVID-19病例。我们将医疗保健专业人员分为七组,并使用Kaplan-Meier估计计算了三个时期(2020年1月2日- 2020年6月30日,2020年1月7日- 2020年12月31日,2021年1月1日- 2021年6月30日)的累积发病率。我们使用Cox回归在多变量模型中确定危险因素。结果卫生保健工作者中发现新冠肺炎病例8,009例,累计发病率为1.79%;83%为女性,中位年龄40.9岁(四分位数范围31.2 ~ 51.6)。以护理员(53%)和护士(17%)为主,累计发病率最高,分别为2.07% (95%CI, 2.01 ~ 2.13%)和1.82% (95%CI, 1.73 ~ 1.91%)。危险因素为男性(危险比(HR) 1.2;95%CI, 1.1−1.3),外语母语(HR 2.5;95%CI, 2.2−2.9)和出生国(HR 1.2;95%可信区间,1.1−1.4)。有6113 / 8009例(76.3%)的医生通知数据;244/ 6113(4.0%)住院,37/ 6113(0.6%)在重症监护室。结论护士和护理员感染新冠肺炎的风险较高,尤其是男性和外籍专业人员。在培训和实施感染控制措施以保护自己和患者时,应具体解决这一问题。
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引用次数: 0
Multi-drug resistant Pseudomonas aeruginosa: a 2019–2020 single center retrospective case control study 多重耐药铜绿假单胞菌:2019-2020年单中心回顾性病例对照研究
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100296
Ann Fan Yang , Vivian Huang , Jevon Samaroo-Campbell , Michael Augenbraun

Multi-drug resistance in the post COVID-19 world is a growing concern. The objective of this study was to describe temporal trends and explore independent risk factors for the isolation of multi-drug resistant (MDR) P. aeruginosa.

Methods

This was a retrospective case-control study of patients with P. aeruginosa isolates recovered from January 2019 to December 2020. MDR P. aeruginosa was defined as non-susceptibility to at least one agent in three or more anti-pseudomonal antimicrobial categories.

Results

In total, 258 unique isolates were identified. Prolonged hospitalization (P<0.001), prior antibiotic use (P<0.001), and respiratory sources (P<0.001) were strongly associated with the presence of MDR P. aeruginosa. From 2019 to 2020, there was a decrease in the total number of P. aeruginosa isolates but a significant increase in the proportion of MDR P. aeruginosa isolates (P=0.015).

Conclusions

Over a period that coincided with the COVID-19 pandemic, there was an increased proportion of MDR P. aeruginosa isolates from hospitalized patients. Improved identification of patients at risk for MDR P. aeruginosa could facilitate appropriate empiric antibiotic decisions like dual anti-pseudomonal therapy. The features of the COVID-19 outbreak that had a severe impact on patient care and that may have affected drug resistance in other respiratory pathogens should be explored.

COVID-19后世界的多重耐药问题日益令人担忧。本研究的目的是描述多重耐药(MDR)铜绿假单胞菌(P. aeruginosa)分离的时间趋势和探索独立危险因素。方法对2019年1月至2020年12月康复的铜绿假单胞菌患者进行回顾性病例对照研究。耐多药铜绿假单胞菌被定义为对三种或多种抗假单胞菌抗菌药物类别中至少一种药物不敏感。结果共鉴定出258株分离物。长期住院(P<0.001)、既往抗生素使用(P<0.001)和呼吸源(P<0.001)与耐多药铜绿假单胞菌的存在密切相关。从2019年到2020年,铜绿假单胞菌分离株总数有所减少,但耐多药铜绿假单胞菌分离株比例显著增加(P=0.015)。结论在COVID-19大流行期间,从住院患者中分离出耐多药铜绿假单胞菌的比例有所增加。改进对耐多药铜绿假单胞菌风险患者的识别,可以促进适当的经验性抗生素决策,如双重抗假单胞菌治疗。应探索COVID-19疫情对患者护理产生严重影响并可能影响其他呼吸道病原体耐药性的特点。
{"title":"Multi-drug resistant Pseudomonas aeruginosa: a 2019–2020 single center retrospective case control study","authors":"Ann Fan Yang ,&nbsp;Vivian Huang ,&nbsp;Jevon Samaroo-Campbell ,&nbsp;Michael Augenbraun","doi":"10.1016/j.infpip.2023.100296","DOIUrl":"https://doi.org/10.1016/j.infpip.2023.100296","url":null,"abstract":"<div><p>Multi-drug resistance in the post COVID-19 world is a growing concern. The objective of this study was to describe temporal trends and explore independent risk factors for the isolation of multi-drug resistant (MDR) <em>P. aeruginosa.</em></p></div><div><h3>Methods</h3><p>This was a retrospective case-control study of patients with <em>P. aeruginosa</em> isolates recovered from January 2019 to December 2020. MDR <em>P. aeruginosa</em> was defined as non-susceptibility to at least one agent in three or more anti-pseudomonal antimicrobial categories.</p></div><div><h3>Results</h3><p>In total, 258 unique isolates were identified. Prolonged hospitalization (<em>P</em>&lt;0.001), prior antibiotic use (<em>P</em>&lt;0.001), and respiratory sources (<em>P</em>&lt;0.001) were strongly associated with the presence of MDR <em>P. aeruginosa</em>. From 2019 to 2020, there was a decrease in the total number of <em>P. aeruginosa</em> isolates but a significant increase in the proportion of MDR <em>P. aeruginosa</em> isolates (<em>P</em>=0.015).</p></div><div><h3>Conclusions</h3><p>Over a period that coincided with the COVID-19 pandemic, there was an increased proportion of MDR <em>P. aeruginosa</em> isolates from hospitalized patients. Improved identification of patients at risk for MDR <em>P. aeruginosa</em> could facilitate appropriate empiric antibiotic decisions like dual anti-pseudomonal therapy. The features of the COVID-19 outbreak that had a severe impact on patient care and that may have affected drug resistance in other respiratory pathogens should be explored.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49855818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Pseudo-outbreak of haemodiafiltration dialysis fluid contamination: results of a detailed epidemiologic investigation 假性爆发血液滤过透析液污染:详细的流行病学调查结果
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100292
Renée Lévesque , Patrice Savard , Bernard Canaud

Background

Compliance with dialysis fluid ultrapurity standards is a paramount for online modalities. More than 200 dialysis fluid samples have been analyzed monthly for years in our two dialysis units, with compliant microbiological results until mid-2020.

Aim

In mid-2020, an unusual occurrence (30%) of contaminated dialysis fluids in dialysis units led us to investigate to determine the source.

Methods

Microbiological methods for aquaphilic bacteria culturing and endotoxin detection in dialysis fluids were routinely performed on a monthly basis for all dialysis machines. As the contamination appeared randomly and almost simultaneously in our two units without any routine change or febrile syndrome, we searched for a common cause. Supplier's sampling kits as well as microbiological laboratory procedures were scrupulously investigated.

Findings

21 out of 30 sampling bags filled with sterile water brought back numerous fungi and bacteria. Laboratory's investigation, through the negative control tests performed routinely, exonerated the lab. All batches of bags analyzed later showed variable levels of contamination according to their transport/storage mode or date of manufacturing. Analyses performed by the supplier – methods complying with the medical device's standards but different from those recommended for dialysis fluids purity – remained negative.

Conclusion

Our investigation revealed that the contamination of our sampling kits came presumably from the manufacturer's supplying chain. Such false-positive results findings, created serious safety issues and disturbed clinical activities since positive machines were quarantined. Furthermore, it raised a serious concern about manufacturing, microbiological checking and shipping methods for the medical device industry that deserve further attention.

符合透析液超纯度标准是在线模式的重中之重。多年来,我们的两个透析单元每月分析200多个透析液样本,直到2020年中期,微生物学结果都符合要求。在2020年中期,透析单位中不寻常地出现了受污染的透析液(30%),这促使我们进行调查以确定来源。方法采用微生物学方法对所有透析机透析液进行亲水细菌培养和内毒素检测,每月例行一次。由于污染随机出现,几乎同时出现在我们的两个单位,没有任何常规变化或发热综合征,我们寻找一个共同的原因。供应商的取样包和微生物实验室程序都经过严格调查。在30个装满无菌水的取样袋中,有21个带回了大量的真菌和细菌。实验室的调查,通过常规的阴性对照试验,证明了实验室的清白。随后分析的所有批次的袋子都显示出不同程度的污染,这取决于它们的运输/储存方式或制造日期。供应商进行的分析——方法符合医疗设备的标准,但与透析液纯度的推荐方法不同——仍为阴性。结论我们的调查显示,我们的抽样试剂盒的污染可能来自制造商的供应链。这种假阳性结果的发现造成了严重的安全问题,并扰乱了临床活动,因为阳性机器被隔离了。此外,它对医疗器械行业的制造、微生物检查和运输方法提出了严重关切,值得进一步关注。
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引用次数: 1
Detection of SARS-CoV-2 RNA in exhaled breath and its potential for prevention measures 呼气中严重急性呼吸系统综合征冠状病毒2型核糖核酸的检测及其预防措施的潜力。
Q2 Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.infpip.2023.100299
Madiha Malik, Thomas Kunze

Background

To propose infection prevention measures it is essential to understand the dynamics of SARS-CoV-2 shedding, particularly in asymptomatic patients. This report compares the viral load progression in exhaled breath (EB) with the symptom severity. We aim to evaluate the adequacy of symptom assessment regarding the infectivity level of individuals.

Methods

We observed infected patients since their first positive test during hospitalization. EB samples were collected on days 1, 3, 5, 7, 10, 12 and 14 of hospitalization using a filter-based device. After extraction, viral loads were quantified with qRT-PCR. The infection trajectory was documented after symptom onset.

Case Presentation and Discussion

A 34-year old patient showed mild symptoms, e.g. fever, cough, headache, muscle pain and loss of taste and smell across trajectory of infection (Case 1). The viral loads emitted via exhaling were nearly constant and ranged from 8.6 x 103 and 4.1 x 104 RNA copies per hour. After the infection, the patient developed a pneumonia. The second case of a 65-year old patient depicted an asymptomatic infection trajectory for 14 days after the first diagnosis (Case 2). Nevertheless, the patient exhaled up to 2 x 105 SARS-CoV-2 virus copies hourly, approximately 10 fold higher than measured for Case 1.

Conclusion

Symptomatic and asymptomatic COVID-19 patients exhale distinctive amounts of SARS-CoV-2 not necessarily correlating with symptom severity. Particularly, asymptomatic patients might show higher EB viral shedding. Therefore, EB testing should be included in infection prevention measures as it has high potential to reveal the most infectious individuals regardless of their symptoms during infection.

背景:为了提出感染预防措施,了解严重急性呼吸系统综合征冠状病毒2型脱落的动态至关重要,尤其是在无症状患者中。本报告比较了呼气中病毒载量的进展与症状的严重程度。我们的目的是评估关于个体传染性水平的症状评估的充分性。方法:我们观察了感染患者在住院期间首次检测呈阳性后的情况。使用基于过滤器的设备在住院的第1、3、5、7、10、12和14天采集EB样本。提取后,用qRT-PCR对病毒载量进行定量。症状出现后记录感染轨迹。病例介绍和讨论:一名34岁的患者在感染过程中表现出轻微症状,如发烧、咳嗽、头痛、肌肉疼痛以及味觉和嗅觉丧失(病例1)。通过呼气释放的病毒载量几乎恒定,范围为每小时8.6 x 103和4.1 x 104个RNA拷贝。感染后,病人患上了肺炎。第二例65岁患者在首次诊断后14天内出现无症状感染轨迹(病例2)。尽管如此,患者每小时呼出多达2×105个SARS-CoV-2病毒拷贝,比病例1的测量值高出约10倍。结论:有症状和无症状的新冠肺炎患者呼出不同数量的SARS-CoV-2,不一定与症状严重程度相关。特别是,无症状患者可能表现出较高的EB病毒脱落。因此,EB检测应包括在感染预防措施中,因为它很有可能揭示最具传染性的个体,无论他们在感染期间的症状如何。
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引用次数: 0
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Infection Prevention in Practice
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