and Mupirocin –resistance (MRSA) are considered as the important hospital pathogens. One of the important resources of this organism in nosocomial infection is related to hospital personnel. The objective of this research was is to investigate the frequency of MRSA gene in staphylococcus aureus isolated from Hospital staff in Darab by multiplex PCR. In this study, 150 hospital staffs were investigated and specimens of staphylococcus aureus which isolated from those were confirmed by biochemical and microbial methods. The disk diffusion method was used to determine the susceptibility of the isolates to methicillin and mupirocin antibiotic. Then, the presence of mec-A, mup and nuc genes were investigated by using of Multiplex PCR method. of Hospital was relatively low. However, permanent control of the carriers and treatment of them can prevent the spread of this bacterium and its associated infections.
{"title":"Methicillin and Mupirocin resistance genes in Staphylococcus aureus isolated from hospital staff in Darab by using of Multiplex","authors":"H. jamali","doi":"10.52547/jmj.17.3.35","DOIUrl":"https://doi.org/10.52547/jmj.17.3.35","url":null,"abstract":"and Mupirocin –resistance (MRSA) are considered as the important hospital pathogens. One of the important resources of this organism in nosocomial infection is related to hospital personnel. The objective of this research was is to investigate the frequency of MRSA gene in staphylococcus aureus isolated from Hospital staff in Darab by multiplex PCR. In this study, 150 hospital staffs were investigated and specimens of staphylococcus aureus which isolated from those were confirmed by biochemical and microbial methods. The disk diffusion method was used to determine the susceptibility of the isolates to methicillin and mupirocin antibiotic. Then, the presence of mec-A, mup and nuc genes were investigated by using of Multiplex PCR method. of Hospital was relatively low. However, permanent control of the carriers and treatment of them can prevent the spread of this bacterium and its associated infections.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47521189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Increased expression of Bcl-2 gene in cancer cells has anti-apoptotic effect. In the present study the effect of Spirulina extract on gene expression in cancer cell line was investigated. In this cell The brain cancer cell line was procured and cultivated Iranian Biological Cells were divided into two treatment and control groups. Spirulina alga extract was affected on the treatment group and its toxicity was measured using MTT assay (compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor). The reference gene in this study was GAPDH. RNA was extracted from control and control cells and cDNA was made. The expression of genes was evaluated using ∆∆Ct in Real-time PCR. The result of MTT assay showed that the IC50 (Median Inhibition Concentration) value was 10.01 mg/ml for the 48 hour treatment. The result of Real-time PCR showed that the expression of Bcl-2 and the anti-apoptotic gene significantly decreased 3 folds in treatment group. Conclusion: According to the results of MTT assay and Real-time PCR, the expression of Bcl-2 significantly decreased 3 times in treatment group.
{"title":"The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line","authors":"M. Kavousi, delnaz fatemi","doi":"10.52547/jmj.17.3.17","DOIUrl":"https://doi.org/10.52547/jmj.17.3.17","url":null,"abstract":": U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Increased expression of Bcl-2 gene in cancer cells has anti-apoptotic effect. In the present study the effect of Spirulina extract on gene expression in cancer cell line was investigated. In this cell The brain cancer cell line was procured and cultivated Iranian Biological Cells were divided into two treatment and control groups. Spirulina alga extract was affected on the treatment group and its toxicity was measured using MTT assay (compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor). The reference gene in this study was GAPDH. RNA was extracted from control and control cells and cDNA was made. The expression of genes was evaluated using ∆∆Ct in Real-time PCR. The result of MTT assay showed that the IC50 (Median Inhibition Concentration) value was 10.01 mg/ml for the 48 hour treatment. The result of Real-time PCR showed that the expression of Bcl-2 and the anti-apoptotic gene significantly decreased 3 folds in treatment group. Conclusion: According to the results of MTT assay and Real-time PCR, the expression of Bcl-2 significantly decreased 3 times in treatment group.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41399813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khadijeh Babakhani, S. Vaziri, Bouk Tajeri, F. Lotfi-Kashani, tahereh ranjbarpour
{"title":"Effect of Integrative Psychotherapy on Body Image, Mindful Eating and Satisfaction of Therapy and Therapist in Obese women","authors":"Khadijeh Babakhani, S. Vaziri, Bouk Tajeri, F. Lotfi-Kashani, tahereh ranjbarpour","doi":"10.52547/jmj.17.3.7","DOIUrl":"https://doi.org/10.52547/jmj.17.3.7","url":null,"abstract":"","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44035519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Pourahmadi, reyhaneh rouhijahromi, Mohsen Zargar, B. Haghighi, F. Moradi, Z. Faraji
{"title":"Identification of Campylobacter species in Jahrom slaughterhouse chickens in 2016-17","authors":"M. Pourahmadi, reyhaneh rouhijahromi, Mohsen Zargar, B. Haghighi, F. Moradi, Z. Faraji","doi":"10.52547/jmj.17.3.1","DOIUrl":"https://doi.org/10.52547/jmj.17.3.1","url":null,"abstract":"","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49241430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Zekavat, F. Mehrabipour, N. Shakibazad, Z. Fattah, S. Haghpanah
{"title":"Evaluation of intima-media thickness of common carotid artery in thalassemia patients compared to healthy individuals","authors":"O. Zekavat, F. Mehrabipour, N. Shakibazad, Z. Fattah, S. Haghpanah","doi":"10.52547/jmj.17.2.1","DOIUrl":"https://doi.org/10.52547/jmj.17.2.1","url":null,"abstract":"","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43419119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
a clear band of around 35 kDa in SDS-PAGE and was detectable using anti-Neff antibody in western blotting. Conclusion: Our results showed that the Nef-MPER-V3 protein was successfully expressed in prokaryotic system and purified protein may provide the antigen for future experiments for immunogenicity evaluation in mice is currently undertaken.
{"title":"Cloning, Optimization of Expression Condition and Purification of the Recombinant Nef- MPER-V3 Protein of HIV-1 in Prokaryotic Expression System","authors":"A. Faghih, S. M. Sadat, A. Bolhassani, S. Irani","doi":"10.52547/jmj.17.2.46","DOIUrl":"https://doi.org/10.52547/jmj.17.2.46","url":null,"abstract":"a clear band of around 35 kDa in SDS-PAGE and was detectable using anti-Neff antibody in western blotting. Conclusion: Our results showed that the Nef-MPER-V3 protein was successfully expressed in prokaryotic system and purified protein may provide the antigen for future experiments for immunogenicity evaluation in mice is currently undertaken.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43062767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protective effect of melatonin on pituitary- gonadal axis hormones and spermatogenesis in sertraline-induced adult Balb/c mice","authors":"M. Goudarzi, A. Pilehvarian, A. Karimi","doi":"10.52547/jmj.17.2.54","DOIUrl":"https://doi.org/10.52547/jmj.17.2.54","url":null,"abstract":"","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42039478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer","authors":"Zahra Tahmasebi Fard","doi":"10.52547/jmj.17.2.8","DOIUrl":"https://doi.org/10.52547/jmj.17.2.8","url":null,"abstract":"","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44224967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kazemi, Fatemeh Atashi, Reyhneh Rouhi jahromi, D. Javeshghani, Pejman Hamedi-Asl, Fatemeh Vahidinezhad, Abdolrahman Mohtashemifar, Zahra Hooshyar, Z. Rezaee, Nazila Shiravani, Pegah Eidizadeh, Farnoosh Hatami
: Introduction: Most cases of blood transfusion-associated bacterial sepsis are caused by platelet concentrates (PCs). Screening of PCs is carried out in the Iranian quality control plans as it is essential for detection of bacterial contamination. With regard to quality control, this study investigated the frequency of bacterial contamination of PCs through Gram staining and microbial culture methods. In per for in agar and agar under aerobic and microaerophilic for detection of both Gram positive and negative cocci and Gram positive bacteria. Cultures were also made in MacConkey medium under aerobic conditions to identify Gram negative bacilli. In cases of bacterial growth and colony formation, differential tests were performed to detect the bacteria. bags collected from donors in Jahrom Blood Transfusion Organization, from which two platelet cord (880 samples) had been isolated on the first and third days. Accordingly, a contamination frequency of 0.23% was detected for platelet products in Jahrom city. Performing staining and culture from all of the platelet cords before are is a good to ensure safety and contamination quality
{"title":"Frequency of bacterial contamination of platelet products in blood transfusion organization of Jahrom in 2016-17","authors":"A. Kazemi, Fatemeh Atashi, Reyhneh Rouhi jahromi, D. Javeshghani, Pejman Hamedi-Asl, Fatemeh Vahidinezhad, Abdolrahman Mohtashemifar, Zahra Hooshyar, Z. Rezaee, Nazila Shiravani, Pegah Eidizadeh, Farnoosh Hatami","doi":"10.52547/jmj.17.1.52","DOIUrl":"https://doi.org/10.52547/jmj.17.1.52","url":null,"abstract":": Introduction: Most cases of blood transfusion-associated bacterial sepsis are caused by platelet concentrates (PCs). Screening of PCs is carried out in the Iranian quality control plans as it is essential for detection of bacterial contamination. With regard to quality control, this study investigated the frequency of bacterial contamination of PCs through Gram staining and microbial culture methods. In per for in agar and agar under aerobic and microaerophilic for detection of both Gram positive and negative cocci and Gram positive bacteria. Cultures were also made in MacConkey medium under aerobic conditions to identify Gram negative bacilli. In cases of bacterial growth and colony formation, differential tests were performed to detect the bacteria. bags collected from donors in Jahrom Blood Transfusion Organization, from which two platelet cord (880 samples) had been isolated on the first and third days. Accordingly, a contamination frequency of 0.23% was detected for platelet products in Jahrom city. Performing staining and culture from all of the platelet cords before are is a good to ensure safety and contamination quality","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46184489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Pooyan, N. Kazemi-Pour, Farokh Rokhbakhsh-Zamin
Urinary tract infections are the common medical problems in pregnant women and associated bacteria produce biofilm which protects them from antibiotics. Aim of this study was to determine the pattern of antibiotic resistance and biofilm formation by Escherichia coli and Klebsiellapneumoniae isolated from urine of pregnant women in Kerman, Iran. 107 urine samples were collected from pregnant women. Identification was performed by biochemical tests. Antibiotic sensitivity by disc diffusion method and minimum inhibitory concentration (MIC) by agar dilution method were performed. The cell surface hydrophobicity (CSH) behavior of those strains and their ability to form biofilm on glass and propylene surfaces was evaluated in static and shake conditions. The effect of antibiotics on biofilm formation ability was also determined on the catheter surface. From 80 positive cases, 35 isolates of Escherichia coli and 15 isolates of Klebsiella pneumoniae were selected for this research. The Maximum resistance and sensitivity of isolates were observed about amoxicillin and imipenem, respectively. All isolates with CSH higher than 70% showed better biofilm formation even though on propylene in shaking state. Treatment of culture with 0.5 MIC (µg/ml) and 0.25 MIC (µg/ml) of selected antibiotics significantly reduced the adhesion ability of isolates. way against UTI in pregnancy.
{"title":"Biofilm formation and antimicrobial resistance of uropathogenic Escherichia coli and Klebsiella pneumonia strains isolated from pregnant women in Kerman, Iran","authors":"S. Pooyan, N. Kazemi-Pour, Farokh Rokhbakhsh-Zamin","doi":"10.52547/jmj.17.1.15","DOIUrl":"https://doi.org/10.52547/jmj.17.1.15","url":null,"abstract":"Urinary tract infections are the common medical problems in pregnant women and associated bacteria produce biofilm which protects them from antibiotics. Aim of this study was to determine the pattern of antibiotic resistance and biofilm formation by Escherichia coli and Klebsiellapneumoniae isolated from urine of pregnant women in Kerman, Iran. 107 urine samples were collected from pregnant women. Identification was performed by biochemical tests. Antibiotic sensitivity by disc diffusion method and minimum inhibitory concentration (MIC) by agar dilution method were performed. The cell surface hydrophobicity (CSH) behavior of those strains and their ability to form biofilm on glass and propylene surfaces was evaluated in static and shake conditions. The effect of antibiotics on biofilm formation ability was also determined on the catheter surface. From 80 positive cases, 35 isolates of Escherichia coli and 15 isolates of Klebsiella pneumoniae were selected for this research. The Maximum resistance and sensitivity of isolates were observed about amoxicillin and imipenem, respectively. All isolates with CSH higher than 70% showed better biofilm formation even though on propylene in shaking state. Treatment of culture with 0.5 MIC (µg/ml) and 0.25 MIC (µg/ml) of selected antibiotics significantly reduced the adhesion ability of isolates. way against UTI in pregnancy.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41779783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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