Antibiotic growth promoters (AGP) are widely used in animal feed for feed efficiency, stimulate growth, productivity and minimize mortality by preventing infection, but now its use has been officially banned by the government. As a result of the ban, many farmers or animal feed producer seek solutions in various ways such as the addition of herbs, probiotics, and a combination of both, but there are still few reports on the role of herbs, probiotics and a combination of both for alternative substitutes for AGP. This study aims to determine the role of herbs in the growth of probiotics. Herbs used ginger, turmeric, galingale, wild ginger, and temuireng, while the probiotics used are Bifidobacterium longum, B. bifidum, Bacillus sp., and Saccharomyces cereviciae. The benefits of herbs in supporting probiotics were tested using the disc diffusion method. Chloramphenicol disc ware used as control positive. Observation and measurement of the growth zone of probiotics were carried out after incubation. The results of this study prove that herbs do not inhibit the growth of probiotics. Herbal content can be a prebiotic candidate that can support the growth of probiotics. The combination of herbal-probiotics can potentially be an AGP substitute in animal feed.
{"title":"Study In Vitro: Kombinasi Herbal dan Probiotik sebagai Alternatif Antibiotic Growth Promoter","authors":"V. C. Prakasita","doi":"10.31957/jbp.1166","DOIUrl":"https://doi.org/10.31957/jbp.1166","url":null,"abstract":"Antibiotic growth promoters (AGP) are widely used in animal feed for feed efficiency, stimulate growth, productivity and minimize mortality by preventing infection, but now its use has been officially banned by the government. As a result of the ban, many farmers or animal feed producer seek solutions in various ways such as the addition of herbs, probiotics, and a combination of both, but there are still few reports on the role of herbs, probiotics and a combination of both for alternative substitutes for AGP. This study aims to determine the role of herbs in the growth of probiotics. Herbs used ginger, turmeric, galingale, wild ginger, and temuireng, while the probiotics used are Bifidobacterium longum, B. bifidum, Bacillus sp., and Saccharomyces cereviciae. The benefits of herbs in supporting probiotics were tested using the disc diffusion method. Chloramphenicol disc ware used as control positive. Observation and measurement of the growth zone of probiotics were carried out after incubation. The results of this study prove that herbs do not inhibit the growth of probiotics. Herbal content can be a prebiotic candidate that can support the growth of probiotics. The combination of herbal-probiotics can potentially be an AGP substitute in animal feed.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"294 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115352488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.
{"title":"Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.","authors":"Wuri Artikasari, Emantis Rosa, Bambang Irawan","doi":"10.31957/jbp.833","DOIUrl":"https://doi.org/10.31957/jbp.833","url":null,"abstract":"DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. \u0000 \u0000Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114894708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maize is one of food commodity in Indonesia. Maize pioneer 21 is a kind of high-yielding maize variety in Indonesia. One of the way able to be conducted to improve growth of maize plant is with fertilizing combination of nanosilica and NPK. This research aim to know the influence of fertilizing combination of nanosilica and NPK toward improving growth of maize plant and to know optimal concentration of combination nanosilica fertilizer and NPK in corn plants growth. Nanosilica is fertilizer that contains micronutrients Si while the NPK fertilizer contains macro nutrients NPK. Research conducted with Completely Ramdomized Design (CRD) with 6 combination factor: P0 (control), P1 (100% nanosilica), P2 (75% nanosilica + 25% NPK), P3 (50% nanosilica + 50% NPK), P4 (25% nanosilica + 75% NPK) and P5 (100% NPK) each has 3 replications. Parameter perceived by hat is high of plants, high of leaf, amount of leaf, wet heavy of maize plants and dry heavy of maize plant. Data to be analysed with Analysis of variance (ANOVA) level of signification 95%, continued by Duncan Multiple Range Test (DMRT) level of signification 95%. The results showed that the combination of nanosilica fertilization and NPK significantly affected the wet weight and dry weight of the Maize P-21. The optimal combination for the growth of Maize P-21 is the treatment of P4 (25% nanosilica+ 75% NPK). Key words: Zea mays L var. pioneer 21; combination; fertilizing; nanosilica; NPK
{"title":"Kombinasi Pupuk Nanosilika dan NPK Terhadap Pertumbuhan Tanaman Jagung (Zea mays L var. pioneer 21)","authors":"Oktivani D.P. Hayati, E. Prihastanti, E. Hastuti","doi":"10.31957/JBP.896","DOIUrl":"https://doi.org/10.31957/JBP.896","url":null,"abstract":"Maize is one of food commodity in Indonesia. Maize pioneer 21 is a kind of high-yielding maize variety in Indonesia. One of the way able to be conducted to improve growth of maize plant is with fertilizing combination of nanosilica and NPK. This research aim to know the influence of fertilizing combination of nanosilica and NPK toward improving growth of maize plant and to know optimal concentration of combination nanosilica fertilizer and NPK in corn plants growth. Nanosilica is fertilizer that contains micronutrients Si while the NPK fertilizer contains macro nutrients NPK. Research conducted with Completely Ramdomized Design (CRD) with 6 combination factor: P0 (control), P1 (100% nanosilica), P2 (75% nanosilica + 25% NPK), P3 (50% nanosilica + 50% NPK), P4 (25% nanosilica + 75% NPK) and P5 (100% NPK) each has 3 replications. Parameter perceived by hat is high of plants, high of leaf, amount of leaf, wet heavy of maize plants and dry heavy of maize plant. Data to be analysed with Analysis of variance (ANOVA) level of signification 95%, continued by Duncan Multiple Range Test (DMRT) level of signification 95%. The results showed that the combination of nanosilica fertilization and NPK significantly affected the wet weight and dry weight of the Maize P-21. The optimal combination for the growth of Maize P-21 is the treatment of P4 (25% nanosilica+ 75% NPK). Key words: Zea mays L var. pioneer 21; combination; fertilizing; nanosilica; NPK ","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115742341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coffee bean are rich of secondary metabolits that able to inhibit free radical compounds. This antioxidant activity may reduce many diseases correlated with it. The aims of this study were to determined the phytochemical content and antioxidant activity of roasted coffee bean from Wamena and Moenemani regency, Papua. Roasted coffee beans were extracted by maceration for 24 hr with methanol. Harborne standard method was used for the phytochemical analysis and DPPH assay was used to determine the antioxidant activity. IC50 was measured by spectrophotometric assay using spectrophotometer Uv-Vis at 517 nm wavelenghth. Result showed that both Arabica roasted coffee beans from Wamena and Moanemany had the capacity to inhibit free radical at 61,71% and 69,7% with IC50 at 107,97 and 100,91 ppm, respectively . Phytochemical investigation revealed that the bioactive compounds from Moanemani and Wamena coffee beans were similar, which composed of alkaloids, flavonoids, terpenoids, saponins, and tanins. In conclusion, the methanolic extract of roasted Arabica coffee beans from Wamena and Moanemani can be used as the source of natural antioxidant.Keywords: Arabica roasted coffee beans; phytochemical; antioxidant; DPPH method.
{"title":"Skrining Fitokimia dan Uji Antioksidan Ekstrak Biji Kopi Sangrai Jenis Arabika (Coffea arabica) Asal Wamena dan Moanemani, Papua","authors":"Septiani Mangiwa, A. Maryuni","doi":"10.31957/JBP.925","DOIUrl":"https://doi.org/10.31957/JBP.925","url":null,"abstract":"Coffee bean are rich of secondary metabolits that able to inhibit free radical compounds. This antioxidant activity may reduce many diseases correlated with it. The aims of this study were to determined the phytochemical content and antioxidant activity of roasted coffee bean from Wamena and Moenemani regency, Papua. Roasted coffee beans were extracted by maceration for 24 hr with methanol. Harborne standard method was used for the phytochemical analysis and DPPH assay was used to determine the antioxidant activity. IC50 was measured by spectrophotometric assay using spectrophotometer Uv-Vis at 517 nm wavelenghth. Result showed that both Arabica roasted coffee beans from Wamena and Moanemany had the capacity to inhibit free radical at 61,71% and 69,7% with IC50 at 107,97 and 100,91 ppm, respectively . Phytochemical investigation revealed that the bioactive compounds from Moanemani and Wamena coffee beans were similar, which composed of alkaloids, flavonoids, terpenoids, saponins, and tanins. In conclusion, the methanolic extract of roasted Arabica coffee beans from Wamena and Moanemani can be used as the source of natural antioxidant.Keywords: Arabica roasted coffee beans; phytochemical; antioxidant; DPPH method.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125542795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
East coast of Surabaya is one of region in Indonesia which contaminated by heavy metals, such as zinc. Zinc is one of essential heavy metals for organisms but be dangerous for human if comsumed in high concentration. So, it is important to to solve the problem to reduce the concentration of zinc in east coast of Surabaya through bioremediation using microorganism. Zinc resistant microorganism could be isolated from zinc contaminated region and be used for bioremediation agent because some microorganism developed resistance mechanism to zinc by bioaccumulation or biosorption. The aims of this study are to isolate and characterize zinc resistant microorganism from east coast of Surabaya. The most resistant microorganism will be observed for the growth in medium supplemented with zinc and without zinc. The potency of the chosen isolate to biosorb of zinc was also analyzed. Water sample from east coast of Surabaya was serries dilluted and innoculated on Luria Bertani Agar medium using spread plated method. Microbial resistance to zinc was analyzed by grown the cell on medium containing various concentration of zinc to determine Minimum Inhibitory Concentration (MIC) value of each microbial isolate. Cell turbidity will be observed in the chosen isolate using spectrophotometer in wave length of 600 nm to get the growth curve. The potency of the chosen isolate to biosorb of zinc was analyzed using atomic absorption spectrophotometer. From this study, six bacteria and three yeast have been isolated from east coast of Surabaya, designated as PmbZn1, PmbZn2, PmbZn3, PmbZn4, PmbZn5, PmbZn6, PmbZn7, PmbZn8, dan PmbZn9. All isolates could grow on medium containing 8 mM ZnSO4. The most resistant bacterial was isolate PmbZn5 with the MIC of 9 mM ZnSO4. The potency of the isolate to biosorb of zinc was 94,79 and 93,57% in medium containing 4 mM and 7 mM ZnSO4, respectively.Key words: Bacteria, biosorb, resistant, yeast, zinc.
泗水东海岸是印尼锌等重金属污染严重的地区之一。锌是生物必需的重金属之一，但过量摄入会对人体造成危害。因此，利用微生物进行生物修复，降低泗水东部沿海地区锌的浓度具有重要意义。耐锌微生物可以从锌污染地区分离出来，并用于生物修复剂，因为一些微生物通过生物积累或生物吸附对锌产生了抗性机制。本研究的目的是分离和鉴定泗水东海岸的耐锌微生物。在加锌和不加锌的培养基中，微生物的抗性最强。并分析了所选分离物对锌的生物吸附力。对泗水东岸水样进行一系列稀释，用涂布法接种于贝氏Luria Bertani琼脂培养基上。通过在含不同浓度锌的培养基上培养细胞，分析微生物对锌的耐药性，确定各分离菌的最低抑菌浓度(MIC)。用分光光度计在600 nm波长下观察所选分离物的细胞浊度，得到生长曲线。用原子吸收分光光度计分析了所选分离物对锌的生物吸附剂效价。从泗水东海岸分离到6株细菌和3株酵母菌，分别鉴定为PmbZn1、PmbZn2、PmbZn3、PmbZn4、PmbZn5、PmbZn6、PmbZn7、PmbZn8和PmbZn9。所有菌株均能在含ZnSO4 8 mM的培养基上生长。菌株PmbZn5抗性最强，MIC为9 mM ZnSO4。在含4 mM和7 mM ZnSO4的培养基中，分离物对锌的生物吸附力分别为94.79%和93.57%。关键词:细菌，生物吸收剂，抗性，酵母，锌。
{"title":"Potensi Mikroorganisme Resisten dari Pantai Timur Surabaya Sebagai Biosorben Seng","authors":"Wahyu Irawati","doi":"10.31957/JBP.878","DOIUrl":"https://doi.org/10.31957/JBP.878","url":null,"abstract":"East coast of Surabaya is one of region in Indonesia which contaminated by heavy metals, such as zinc. Zinc is one of essential heavy metals for organisms but be dangerous for human if comsumed in high concentration. So, it is important to to solve the problem to reduce the concentration of zinc in east coast of Surabaya through bioremediation using microorganism. Zinc resistant microorganism could be isolated from zinc contaminated region and be used for bioremediation agent because some microorganism developed resistance mechanism to zinc by bioaccumulation or biosorption. The aims of this study are to isolate and characterize zinc resistant microorganism from east coast of Surabaya. The most resistant microorganism will be observed for the growth in medium supplemented with zinc and without zinc. The potency of the chosen isolate to biosorb of zinc was also analyzed. Water sample from east coast of Surabaya was serries dilluted and innoculated on Luria Bertani Agar medium using spread plated method. Microbial resistance to zinc was analyzed by grown the cell on medium containing various concentration of zinc to determine Minimum Inhibitory Concentration (MIC) value of each microbial isolate. Cell turbidity will be observed in the chosen isolate using spectrophotometer in wave length of 600 nm to get the growth curve. The potency of the chosen isolate to biosorb of zinc was analyzed using atomic absorption spectrophotometer. From this study, six bacteria and three yeast have been isolated from east coast of Surabaya, designated as PmbZn1, PmbZn2, PmbZn3, PmbZn4, PmbZn5, PmbZn6, PmbZn7, PmbZn8, dan PmbZn9. All isolates could grow on medium containing 8 mM ZnSO4. The most resistant bacterial was isolate PmbZn5 with the MIC of 9 mM ZnSO4. The potency of the isolate to biosorb of zinc was 94,79 and 93,57% in medium containing 4 mM and 7 mM ZnSO4, respectively.Key words: Bacteria, biosorb, resistant, yeast, zinc.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125766927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna S. I. Kawulur, Ivon Ayomi, Melda Suebu, Muhammad F. Rokhmad, M. R. Pardi
Infectious vectors are generally sensitive to environmental change, including climatic factor. Climate may affect the ability of vector in transmitting the disease. The purpose of this study was to determine the effect of climatic factors on density of Anopheles farauti Laveran in coastal and marsh ecosystems. The mosquito was collected by using human landing collection method. The climatic data recorded during the research were air suhue, wind velocity and humidity, while the rainfall rate data was obtained from the Provincial office of Meteorology and Geophysics Agency Suhue of Papua Province. The result showed that Anopheles farauti was constantly found during the study period as air suhue, humidity, rainfall and wind velocity fluctuated. This result suggest that during the data collection climatic factors did not significantly affect the presence and density of Anopheles farauti. Key words: climatic factor; mosquito density; coastal; swamp; Anopheles farauti.
{"title":"Pengaruh Faktor Klimatik Terhadap Kepadatan Nyamuk Anopheles farauti di Ekosistem Pantai dan Rawa Provinsi Papua","authors":"Hanna S. I. Kawulur, Ivon Ayomi, Melda Suebu, Muhammad F. Rokhmad, M. R. Pardi","doi":"10.31957/JBP.945","DOIUrl":"https://doi.org/10.31957/JBP.945","url":null,"abstract":"Infectious vectors are generally sensitive to environmental change, including climatic factor. Climate may affect the ability of vector in transmitting the disease. The purpose of this study was to determine the effect of climatic factors on density of Anopheles farauti Laveran in coastal and marsh ecosystems. The mosquito was collected by using human landing collection method. The climatic data recorded during the research were air suhue, wind velocity and humidity, while the rainfall rate data was obtained from the Provincial office of Meteorology and Geophysics Agency Suhue of Papua Province. The result showed that Anopheles farauti was constantly found during the study period as air suhue, humidity, rainfall and wind velocity fluctuated. This result suggest that during the data collection climatic factors did not significantly affect the presence and density of Anopheles farauti. Key words: climatic factor; mosquito density; coastal; swamp; Anopheles farauti.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130932433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sumardi, S. Farisi, C. N. Ekowati, Rizka Oktavia
This study aims to isolate Bacillus that can fight the growth of Vibrio harveyi . Based on the results of the inter-Bacillus competition test show that Bacillus isolates was able to compete and grow with each other on the SWCA media. The challenge test Bacillus bacterial to against Vibrio harveyi bacteria, that Bacillus did not yet produce anti-bacteria on the second day. In the joint culture test method between Bacillus and Vibrio harveyi that Bacillus were able to inhibit the growth of Vibrio harveyi bacteria on the 4th day.
{"title":"Uji Tantang Bakteri Bacillus Kandidat Probiotik secara Invitro terhadap Bakteri Vibrio harveyi","authors":"S. Sumardi, S. Farisi, C. N. Ekowati, Rizka Oktavia","doi":"10.31957/JBP.799","DOIUrl":"https://doi.org/10.31957/JBP.799","url":null,"abstract":"This study aims to isolate Bacillus that can fight the growth of Vibrio harveyi . Based on the results of the inter-Bacillus competition test show that Bacillus isolates was able to compete and grow with each other on the SWCA media. The challenge test Bacillus bacterial to against Vibrio harveyi bacteria, that Bacillus did not yet produce anti-bacteria on the second day. In the joint culture test method between Bacillus and Vibrio harveyi that Bacillus were able to inhibit the growth of Vibrio harveyi bacteria on the 4th day.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127481796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens. Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.
{"title":"Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)","authors":"W. Wijanarka, Sri Pujiyanto, Budi Raharjo","doi":"10.31957/JBP.877","DOIUrl":"https://doi.org/10.31957/JBP.877","url":null,"abstract":"Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens. Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128194735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants respond to various conditions in the surrounding environment, whether favorable conditions or vice versa. Abiotic and biotic factors affect plant responses such as temperature, humidity, salinity, insects and pathogens such as viruses and bacteria. Plants have a defense system in tolerancing stress from the surrounding environment, for example heat shock protein (HSP) is a chaperone protein that plays a role in plant defenses when experiencing stress to the temperature. HSP is classified into six families based on their molecular weight, namely HSP100, HSP90, HSP70, HSP60, HSP40, and small HSP. Each has a role in maintaining the stability of plant metabolism. HSP is especially important for correct protein refolding, preventing degradation and denaturation of protein. Key words: plants; chaperone; heat shock protein; refolding; protein denaturation.
{"title":"Peran Chaperone Pada Tumbuhan: Mini Review","authors":"Ummi Wasilah, D. Perwitasari, Mukhamad Su’udi","doi":"10.31957/JBP.880","DOIUrl":"https://doi.org/10.31957/JBP.880","url":null,"abstract":"Plants respond to various conditions in the surrounding environment, whether favorable conditions or vice versa. Abiotic and biotic factors affect plant responses such as temperature, humidity, salinity, insects and pathogens such as viruses and bacteria. Plants have a defense system in tolerancing stress from the surrounding environment, for example heat shock protein (HSP) is a chaperone protein that plays a role in plant defenses when experiencing stress to the temperature. HSP is classified into six families based on their molecular weight, namely HSP100, HSP90, HSP70, HSP60, HSP40, and small HSP. Each has a role in maintaining the stability of plant metabolism. HSP is especially important for correct protein refolding, preventing degradation and denaturation of protein. Key words: plants; chaperone; heat shock protein; refolding; protein denaturation.","PeriodicalId":338791,"journal":{"name":"JURNAL BIOLOGI PAPUA","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131774216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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