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Acute Toxicity of Household-Scale Lebak Batik Industrial Wastewater on Common Carp 勒巴克蜡染工业废水对鲤鱼的急性毒性研究
Pub Date : 2021-12-31 DOI: 10.24042/biosfer.v12i2.9737
Swastika Oktavia, C. Novi
The concentration of Lebak Batik industrial wastewater contains the pollutant element of lead metal that negatively impacts organisms' content in the water. This study was focused on the acute toxicity of household-scale Lebak Batik industrial wastewater on carp. The methods used were waste characteristics test, animal acclimatization, acute toxicity test for 96 hours with 0, 10, 20, 30, 40, and 50% treatments, measurement of environmental parameters, and data analysis using probit analysis. The results showed that the lethal time (LT50) and lethal concentration (LC50) for 96 hours of treatment were 1,8 days and 8,56%, respectively, with a Toxicity Unit Area (TUa) of 11,68. This proved that the wastewater from the Lebak batik industry had a major effect on causing acute toxicity.
乐巴克染染工业废水的浓度中含有对水中生物含量产生负面影响的污染物元素金属铅。本研究主要研究了家庭Lebak蜡染工业废水对鲤鱼的急性毒性。采用废物特性试验、动物适应试验、0、10、20、30、40、50%处理96 h急性毒性试验、环境参数测定和probit分析法进行数据分析。结果表明,96 h的致死时间(LT50)和致死浓度(LC50)分别为1.8 d和8.56%,毒性单位面积(TUa)为11.68。这证明了Lebak蜡染工业的废水对急性毒性有很大的影响。
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引用次数: 0
The Besemah Community’s Indigenous Knowledge of Tebat Bukit Conservation in Tebat Benawa Village Besemah社区在Tebat Benawa村的Tebat Bukit保护土著知识
Pub Date : 2021-12-31 DOI: 10.24042/biosfer.v12i2.9641
Doki Efendi, M. Meilinda, R. Riyanto
This research aims at finding out the form of indigenous knowledge of the Tebat Benawa Community toward Tebat Bukit conservation. The researchers conducted this descriptive study from December 2019 to January 2020. The object of the study was the Tebat Benawa Village Community. The data were collected through literature study, observation, and semi-structured interviews. The results show that the community has five indigenous knowledge in the implementation of Tebat Bukit conservation which is classified into three indicators: the availability of tebat (artificial lake) water, Tebat Bukit, and ghumbus; the use of tebat water called Siring Ayik; tebat and its water management, namely nebasi jalan, ghumbus, and society knowledge transfer in preserving Tebat Bukit through direct experience sharing. The experience sharing is conducted by fishing in tebat, implementing indigenous tradition, socializing with the village administration, giving parental advice, and fostering petatah-petitih (oral tradition on local wisdom).
本研究旨在找出Tebat Benawa社区对Tebat Bukit保护的本土知识形式。研究人员从2019年12月到2020年1月进行了这项描述性研究。研究的对象是Tebat Benawa村社区。通过文献研究法、观察法和半结构化访谈法收集数据。结果表明,该社区在实施Tebat Bukit保护方面拥有五项本土知识,这些知识分为三个指标:Tebat(人工湖)水、Tebat Bukit和ghumbus的可用性;使用被称为Siring Ayik的tebat水;tebat及其水管理,即nebasi jalan、ghumbus和通过直接经验分享保护tebat Bukit的社会知识转移。经验分享是通过在tebat捕鱼、实施土著传统、与村庄行政部门交往、提供父母建议和培养petaah -petitih(关于当地智慧的口头传统)来进行的。
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引用次数: 1
Identification of Bacterial Symbiont Sponge with Antibacterial Activity against Multi Drug Resistant Organism (MDRO) Bacteria from Sea Waters in Sibolga, North Sumatra Indonesia 印尼北苏门答腊岛Sibolga海域对多重耐药菌(MDRO)具有抗菌活性的细菌共生海绵的鉴定
Pub Date : 2021-12-30 DOI: 10.24042/biosfer.v12i2.10138
E. Gultom, H. Hasruddin, A. F. Sitompul, Albert D Situmorang, E. Prasetya
This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.
本研究采用描述性数据分析的实验方法。目的利用16S rRNA基因标记分离鉴定具有抗多药耐药菌(MDRO)抑菌潜力的海绵共生菌。根据研究结果,得到两种海绵,即Clathrina sp和Agelas sp,分别有11株和7株共生细菌。从抗菌活性测试结果来看,18株共生菌中仅有4株对MDRO(肺炎克雷伯菌ESBL、铜绿假单胞菌ESBL、卢氏葡萄球菌MRSA)具有抑菌潜力,抑菌区分别为9.3 mm、8.3 mm和8.1 mm。经16S rRNA基因序列鉴定,分离物S1I3、S1I9和A1I3属蜡样芽孢杆菌和副芽孢杆菌。本研究为研究具有抗菌潜力的海绵共生菌提供了重要信息。
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引用次数: 0
Diversity and Abundance of Dragonfly (Anisoptera) and Damselfly (Zygoptera) at Sabo Dam Complang, Kediri, East Java, Indonesia 印度尼西亚东爪哇Kediri Sabo Dam compang蜻蜓(异翅目)和豆豆蝇(双翅目)的多样性和丰度
Pub Date : 2021-12-30 DOI: 10.24042/biosfer.v12i2.9883
M. A. D. Susanto, Okta Fina Arianti
This research aimed to identify and analyze the diversity and abundance of dragonflies and damselflies species at Sabo Dam Complang. The samples were taken using the VES (Visual Encounter Survey) method and two transect methods: line transects, and belt transects. The data obtained were analyzed using the Shannon-Wienner species diversity index (H') and the relative abundance of species (RA). The results showed that at Sabo Dam Complang, the researchers found 11 species with a total of 63 individuals dragonfly of Anisoptera suborder and nine species with a total of 160 individuals from 5 families in the Zygoptera suborder. The results of this research show a diversity index value of 2.59.
本研究旨在鉴定和分析沙波坝区蜻蜓和豆娘种类的多样性和丰度。采用视觉接触调查(Visual Encounter Survey, VES)法和两种样条法:线样条法和带样条法。利用shannon - wiener物种多样性指数(H’)和物种相对丰度(RA)对数据进行分析。结果表明:在沙波坝康朗,共发现异翅目蜻蜓11种,共63只;钩翅目蜻蜓5科9种,共160只;本研究结果表明,其多样性指数为2.59。
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引用次数: 2
The Effect of Tempe Yeast Inoculum Amount on the Quantity and Quality of Cocos nucifera viridis Virgin Coconut Oil (VCO) 酵母接种量对椰油初榨椰子油产量和品质的影响
Pub Date : 2021-12-30 DOI: 10.24042/biosfer.v12i1.8022
Yusdike Adystia, Meidina Rahmawati, K. Anam, R. S. Iswari, P. Dewi
This study aims to determine the effect of tempeh yeast inoculum on the color, odor, and yield in the manufacture of Virgin Coconut Oil (VCO) from Cocos nucifera viridis. This research was conducted in November 2020 in Brebes, Magelang, and Lampung. This research employed the descriptive quantitative method. The population of this experiment was coconut fruit found in Lampung, Brebes, and Magelang. The sample used was green coconut (Cocos nucifera viridis) found in Lampung, Brebes, and Magelang. The working procedure of this research consisted of fermenting the sample and observing the oil produced by Virgin Coconut Oil. This research's data collection techniques were observation, documentation, and products calculation. Based on the observations, the researchers found the highest yield in the experiment of 80 ml cream with an inoculum of 8 grams (30.4%), followed by a volume of 80 ml of cream with a 6-gram inoculum (28.4%). The smallest yield was formed at a volume of 80 ml cream with inoculum 4 grams (14.6%). The VCO obtained in this research had a distinctive aroma of coconut oil and was clear in color. Therefore, the amount of tempeh yeast inoculum affects the color, odor, and yield in the manufacture of Virgin Coconut Oil (VCO) from Cocos nucifera viridis.
本研究旨在研究豆豉酵母接种量对椰油生产初榨椰子油(VCO)的颜色、气味和产量的影响。这项研究于2020年11月在布里布、麦哲朗和楠榜进行。本研究采用描述性定量方法。这个实验的种群是在楠榜、布里布和麦哲朗发现的椰子果实。使用的样本是在楠榜、布里布和麦哲朗发现的绿椰子(Cocos nucifera viridis)。本研究的工作流程包括对样品进行发酵,并观察由初榨椰子油生产出来的油。本研究的数据收集方法为观察、文献记录和产品计算。根据观察,研究人员发现,在接种量为8克的80毫升乳膏的实验中,产率最高(30.4%),其次是接种量为6克的80毫升乳膏(28.4%)。乳膏体积为80 ml,接种量为4 g时产率最小(14.6%)。本研究获得的VCO具有独特的椰子油香气,颜色清晰。因此,豆豉酵母菌接种量的多少影响了从椰油中提取初榨椰子油的颜色、气味和产量。
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引用次数: 1
The Vitamin C Potential of Sorrel Tree (Averrhoa bilimbi) as LATEX Coagulant Sorrel Tree (Averrhoa bilimbi)作为LATEX凝固剂的维生素C潜力
Pub Date : 2021-12-30 DOI: 10.24042/biosfer.v12i2.7877
K. Anam, M. Kamelia, Indah Faridah
This research aims to determine the vitamin C potential of tree sorrels (Averrhoa bilimbi) as a natural latex coagulant. The researchers employed the Completely Randomized Design (CRD) method. The method consisted of three repetitions with five treatments, namely negative control (without clotting) and positive control, by administering tree sorrel extract (Averrhoa bilimbi) with a volume of 5 ml, 10 ml, 15, and 20 ml. The researchers tested the vitamin C levels using UV-VIS Spectrophotometry DR 6000 with a wavelength of 265 nm to 271 nm. Furthermore, the data were analyzed using the One-Way ANOVA statistical test and the LSD to determine the most effective volume as a latex coagulant. The quantitative data were the clotting time and latex weight. The results showed that tree sorrel extract could be used as a latex coagulant. The fastest duration (1.18 minutes) and the highest weight (34.30 grams) of latex were found in the 20 ml volume. At the same time, the lowest yield was at a volume of 5 ml with a clotting time of 5.4 minutes and a weight of 18.62 grams.
本研究旨在确定树酢浆草(Averrhoa bilimbi)作为天然乳胶凝固剂的维生素C潜力。研究人员采用完全随机设计(CRD)方法。该方法分为阴性对照(不凝血)和阳性对照(5 ml、10 ml、15 ml和20 ml) 5个处理,共3次重复。研究人员使用DR 6000紫外可见分光光度法检测维生素C水平,波长为265 nm ~ 271 nm。此外,使用单因素方差分析统计检验和LSD对数据进行分析,以确定作为乳胶混凝剂的最有效体积。定量数据为凝血时间和胶乳重量。结果表明,酢浆草提取物可作为乳胶混凝剂。在20毫升的体积中,乳胶的持续时间最快(1.18分钟),重量最大(34.30克)。同时,最低产率为5 ml,凝血时间为5.4 min,重量为18.62 g。
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引用次数: 0
Developing Student Worksheet Based on System Approach on Water Cycle Topic of Water Conservation Learning at Junior High School 基于系统方法的初中节水学习水循环主题学生作业的开发
Pub Date : 2021-12-30 DOI: 10.24042/biosfer.v12i2.10467
Nur Khalifah, R. Riyanto, Khiron Nazip, M. Meilinda
Air merupakan komponen penting yang harus ada di bumi dan tetap harus di jaga kelestariannya dengan konservasi air yang diajarkan sejak dini, melalui sistem belajar yang kompleks pada materi siklus air dan mengaitkannya dengan berpikir sistem dikembangkanlah LKPD berbasis system approach pada topik siklus air di SMP yang di uji kevalidan dan kepraktisannya melalui metode Developmental Research tipe II oleh Richey Klein (2005) dengan dua tahapan yaitu fase eksplorasi dan uji coba kepada peserta didik. Fase uji coba dilakukan melalui metode lesson study yang diobservasi oleh lima orang observer dengan tujuan untuk mengetahui aktivitas siswa selama pembelajaran berlangsung. Hasil LKPD divalidasi oleh lima orang validator untuk menguji kevalidan LKPD dengan menggunakan skala likert. Teknik analisis data dilakukan dengan analisis data kuantitatif dan kualitatif. Hasil yang diperoleh berupa persentasi uji kevalidan dengan rata-rata78% yang artinya LKPD sudah dalam kategori valid. Kemudian dari hasil uji praktikalitas peserta didik diperoleh hasil rata-rata 81,02% yang menunjukkan kategori sangat praktis. Sehingga di hasilkanlah LKPD yang mempunyai nilai kevalidan dan kepraktisan yang layak digunakan dalam proses pembelajaran siklus air.
水是一种重要的成分,它必须存在于地球上,并通过早期的水保护来保持它的保质性,通过复杂的学习材料和水循环系统程度上认为系统有关LKPD基于系统的进近的话题在等待测试的初中和kepraktisannya水循环通过Developmental研究方法类型II Richey克莱因(2005)和两阶段即对学习者探索和试验阶段。测试阶段是通过五名观察员观察的勒森研究方法进行的,目的是了解学生在学习过程中的活动。五个验证者验证的结果使用likert量表验证有效性。数据分析技术是通过定量和定性数据分析进行的。在平均水平为78%的有效试验中获得的结果是有效的。然后,来自学习者实践测试结果的平均成绩为81.02%,表明这是一个非常实用的类别。因此,它产生于有价值的有效性和实用价值的研究水循环过程。
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引用次数: 3
An Implementation of Rotating Trio Exchange (RTE) Assisted by Coup Card toward Biology Learning Outcome 政变卡辅助下轮换三重奏交换(RTE)对生物学习效果的实现
Pub Date : 2021-12-29 DOI: 10.24042/biosfer.v12i2.9913
Rizki Amalia Nur, N. Nurhidayah
This research aims at finding the effect of the Rotating Trio Exchange (RTE) model assisted by the couple card media on the biology learning outcomes of SMAS DDI Maros. The research method was quantitative with a quasi-experimental and non-equivalent group pretest-posttest design. The research results were obtained through hypothesis testing. The pretest value in the control and the experimental classes was 0.138, where Sig. was higher than α. Therefore, there was no significant learning outcomes difference in the pretest values in both classes. Furthermore, the obtained posttest value was 0.000, where Sig. was lower than α. Therefore, there were significant learning outcomes differences between the two classes.
本研究旨在探讨夫妇卡介质辅助下的旋转三重奏交换(RTE)模型对SMAS DDI Maros生物学学习结果的影响。研究方法采用准实验、非等效组的前测后测设计。研究结果通过假设检验得到。对照组和实验班的前测值为0.138,其中sig大于α。因此,两个班级的前测值没有显著的学习结果差异。得到的后验值为0.000,其中sig < α。因此,两个班的学习结果有显著差异。
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引用次数: 0
The Application of Excretion System E-Module to Improve Science Literacy Skills 排泄系统e模块在提高科学素养技能中的应用
Pub Date : 2021-07-14 DOI: 10.24042/BIOSFER.V12I1.8855
Friska Chairunnisa, Y. Maryuningsih, D. Maknun
The research aims to analyze the application of e-modules in improving scientific literacy skills. This research employed the quasi-experimental with pretest-posttest control design group. The population of this study was eleventh-grade students of SMA Negeri 1 Anjatan which consisted of 15 classes, specifically seven science classes and eight social studies classes. The research sample was taken using random sampling. The researchers collected the research data using observations and tests. Furthermore, the data was analyzed using ANES software and SPSS v.25. The analyzed data were the prerequisite tests data and the t-test data. The results obtained from students' learning activities show the highest learning activities occurred at the data collection stage. There science literacy skills improvement between the control class and the experimental class was different. The experimental class' N-gain value was 0.30 and the control class' N-gain value was 0.2. Furthermore, the result of the sig statistical tests was 0.000, which was lower than 0.655. Therefore, Ho was rejected and Ha was accepted. It can be concluded that there was an improvement difference between the experimental class and the control class regarding scientific literacy skills.
本研究旨在分析电子模块在提高科学素养技能方面的应用。本研究采用准实验、前测后测对照设计组。本研究的人群为SMA Negeri 1 Anjatan的11年级学生,共15个班,其中7个科学班和8个社会研究班。研究样本采用随机抽样。研究人员通过观察和测试收集研究数据。采用ANES软件和SPSS v.25对数据进行分析。所分析的数据为前提检验数据和t检验数据。从学生的学习活动中得到的结果显示,最高的学习活动发生在数据收集阶段。对照组与实验班学生科学素养技能的提高存在差异。实验组n -增益值为0.30,对照组n -增益值为0.2。此外,sig统计检验结果为0.000,低于0.655。因此,何被拒绝,何被接受。由此可见,实验班与对照班在科学素养技能方面存在显著的提高差异。
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引用次数: 4
Preliminary Selection of Potential Probiotic Lactic Acid Bacteria Isolated from Cocoa Beans (Theobroma cacao) 可可豆中潜在益生菌乳酸菌的初步筛选
Pub Date : 2021-07-14 DOI: 10.24042/BIOSFER.V12I1.7128
C. Amarantini, V. C. Prakasita, Maria Trivonia Sema
This study aims to isolate and identify LABs that spontaneously grow fermented cocoa beans that have the potential as probiotics. The isolation was carried out selectively using an MRS agar medium supplemented with 1% CaCO3. LAB's potential as a probiotic was selected based on the acid resistance test, bile salt resistance test, and antimicrobial activity test. The results showed that three isolates of lactic acid bacteria, namely strains K1B1, K2T2, and K3B4 were tolerant of gastric acid's pH conditions (pH 2.5, pH 3, and pH 4). They also can survive the bile salts (0.3%, 0, 5%, and 1%) and have antibacterial characteristics. The three isolates were identified as Lactobacillus Plantarum species (ID 99.9%).
这项研究的目的是分离和鉴定实验室自发生长的发酵可可豆,这些可可豆有可能成为益生菌。采用添加1% CaCO3的MRS琼脂培养基进行选择性分离。通过耐酸试验、耐胆汁盐试验和抗菌活性试验,筛选乳酸菌作为益生菌的潜力。结果表明,3株乳酸菌菌株K1B1、K2T2和K3B4对胃酸的pH条件(pH 2.5、pH 3和pH 4)均有耐受能力,对胆盐(0.3%、0、5%和1%)均有耐受能力,并具有抗菌特性。鉴定为植物乳杆菌(Lactobacillus Plantarum),鉴定率为99.9%。
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引用次数: 0
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Biosfer Jurnal Tadris Biologi
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