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Effect of Rosmarinus officinalis and Origanum majorana extracts on stability of sunflower oil during storage and repeated heating 迷迭香和牛至提取物对葵花籽油在储存和反复加热过程中稳定性的影响
Q3 Agricultural and Biological Sciences Pub Date : 2024-03-01 DOI: 10.1016/j.ocsci.2023.12.006
Ali Sahunie

Both rosemary (Rosmarinus officinalis) and marjoram (Origanum majorana) are abundant in phenolic compounds, exhibiting exceptional antioxidant activity. This study aims to assess the impact of rosemary and marjoram extracts on the stability of sunflower oil during storage and repeated heating. Sunflower oil supplemented with herbal extracts or butylated hydroxytoluene (BHA) at a concentration of 200 ​ppm was stored for six months under light and dark conditions at room temperature. Peroxide value (PV), p-anisidine value (An-V), and total oxidation (TOTOX) value were measured to monitor lipid oxidation progression. A significant difference (P ​< ​0.05) was observed between light and dark storage for all studied samples regarding oxidation parameters. The ethanolic extract of rosemary exhibited higher antioxidant activity compared to BHA and other extracts. Furthermore, sunflower oil supplemented with the ethanolic extract of rosemary underwent weekly treatment at 100 ​°C for 30 ​min over four consecutive weeks. Although all oxidation indicators increased during repeated heating, the addition of rosemary and marjoram extracts as well as BHA significantly reduced these indicators. These findings demonstrate that both rosemary extracts and marjoram extracts can serve as natural antioxidants in edible oils.

迷迭香(Rosmarinus officinalis)和马郁兰(Origanum majorana)都含有丰富的酚类化合物,具有卓越的抗氧化活性。本研究旨在评估迷迭香和马郁兰提取物对葵花籽油在储存和反复加热过程中稳定性的影响。添加了草药提取物或浓度为 200 ppm 的丁基羟基甲苯(BHA)的葵花籽油在室温下的光照和黑暗条件下储存了六个月。测量过氧化值(PV)、对甲氧基苯胺值(An-V)和总氧化值(TOTOX),以监测脂质氧化过程。在氧化参数方面,所有研究样品在光照和黑暗储存条件下均存在明显差异(P < 0.05)。与 BHA 和其他提取物相比,迷迭香乙醇提取物表现出更高的抗氧化活性。此外,添加了迷迭香乙醇提取物的葵花籽油在连续四周内每周在 100 °C 下处理 30 分钟。虽然在反复加热过程中所有氧化指标都会增加,但添加迷迭香和马郁兰提取物以及 BHA 能显著降低这些指标。这些研究结果表明,迷迭香提取物和马郁兰提取物可作为食用油中的天然抗氧化剂。
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引用次数: 0
Application of organic fertilizer for improving soybean production under acidic stress 施用有机肥提高酸性胁迫下的大豆产量
Q3 Agricultural and Biological Sciences Pub Date : 2024-03-01 DOI: 10.1016/j.ocsci.2024.02.001
Putri Gita Lestari , Ayu Oshin Yap Sinaga , David Septian Sumanto Marpaung , Winati Nurhayu , Indah Oktaviani

The presence of acidic soil in rural areas poses difficulties for agricultural production. One factor regulating soil pH is the overuse of inorganic fertilizer. The increased use of fertilizers in soybean production not only raises sustainability concerns but also contributes to soil acidity. Therefore, the use of organic fertilizer could offer a solution for addressing both issues related to soil acidity and sustainability. The purpose of this study was to investigate the manipulation of soil pH using organic fertilizer for soybean production under acidic stress. The planting medium, consisting of a mixture of topsoil, rice husk charcoal, and organic fertilizer (in a ratio of 2:1:1), was supplemented with 0.5 ​g of NPK fertilizer as a basal treatment in each planting medium. To regulate the soil acidity to pH 4, we added FeSO4 and allowed the mixture to incubate for 30 days. The results demonstrate that the application of three types of organic fertilizers chicken manure (P1), oil palm empty bunch fertilizer (P2), and vermicompost (P3) positively impacts the growth of three soybean varieties. The findings indicate that the application of P2 organic fertilizer can increase vegetative growth almost 50% in soybeans on acidic soil, including plant height, leaf count, and root length. Meanwhile, applying P3 organic fertilizer can boost reproductive growth responses in soybeans on acidic soil, such as pod number (from around 0-4 unit to 42–51 unit), grain number (from around 0-5 unit to 88–90 unit), and grain weight (from around 0–0.37 ​g to 12–25 ​g). Organic fertilizer has the potential to regulate soil pH, promoting higher yields of soybeans under acidic stress.

农村地区的酸性土壤给农业生产带来了困难。调节土壤酸碱度的一个因素是过度使用无机肥料。大豆生产中化肥使用量的增加不仅引发了可持续发展的问题,而且还导致了土壤酸化。因此,有机肥的使用可以为解决土壤酸度和可持续性问题提供一种解决方案。本研究的目的是调查在酸性胁迫条件下使用有机肥料调节土壤 pH 值以促进大豆生产的情况。种植介质由表土、稻壳炭和有机肥(比例为 2:1:1)混合而成,在每种种植介质中添加 0.5 克 NPK 肥料作为基础处理。为了将土壤酸度调节到 pH 值 4,我们添加了 FeSO4,并将混合物培养 30 天。结果表明,施用鸡粪(P1)、油棕空束肥(P2)和蛭石(P3)这三种有机肥对三个大豆品种的生长有积极影响。研究结果表明,施用 P2 有机肥可使酸性土壤上大豆的植株生长(包括株高、叶片数和根长)提高近 50%。同时,施用 P3 有机肥可促进酸性土壤上大豆的生殖生长反应,如荚数(从 0-4 个左右增至 42-51 个)、粒数(从 0-5 粒左右增至 88-90 粒)和粒重(从 0-0.37 克左右增至 12-25 克)。有机肥具有调节土壤酸碱度的潜力,可促进大豆在酸性胁迫下获得更高的产量。
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引用次数: 0
Analysis of flavor and widely metabolomics differences in black sesame before and after processing 黑芝麻加工前后的风味和广泛的代谢组学差异分析
Q3 Agricultural and Biological Sciences Pub Date : 2024-03-01 DOI: 10.1016/j.ocsci.2023.12.004
Yini Yang , Linhai Wang , Yunhai Wang , Yuting An , Qi Zhou , Xia Xiang

The objective of this study was to determine the differences of aroma and taste in three black sesame originsbefore and after processing via flavor and widely metabolomics. By analyzing the sensory characteristics and metabolites of raw and treated black sesame from China, Vietnam, and Myanmar, treated Chinese sesame have the most significant change in hardness after thermal processing, low viscosity and was easy to chew. The electronic nose could distinguish between raw and treated sesame due to the aroma distribution. The reason of treated sesame from China was “fragrant” is due to the highest content (2545.50 ​μg/kg) of total pyrazines including 2,5-dimethylpyrazine, 2-ethyl-5-methylpyrazine, 2,3,5-trimethylpyrazine, 3-ethyl-2,5-dimethylpyrazine. 933 metabolites were detected via a wide targeted metabolomics in the taste of raw and treated sesame. Based on the analysis of metabolites related to bitterness, 145 substances were selected. The main bitter contributors may be amino acids, dipeptides and organic acids.

本研究旨在通过风味和广泛的代谢组学研究,确定三种黑芝麻产地在加工前后的香气和口感差异。通过分析中国、越南和缅甸的生黑芝麻和经处理黑芝麻的感官特征和代谢物,结果表明,经处理的中国芝麻在热加工后硬度变化最明显,粘度低,易于咀嚼。电子鼻可根据香气分布区分生芝麻和处理芝麻。经处理的中国芝麻之所以 "香",是因为总吡嗪含量最高(2545.50 μg/kg),包括 2,5-二甲基吡嗪、2-乙基-5-甲基吡嗪、2,3,5-三甲基吡嗪、3-乙基-2,5-二甲基吡嗪。通过广泛的靶向代谢组学研究,在生芝麻和处理过的芝麻的味道中检测到了 933 种代谢物。根据与苦味相关的代谢物分析,选出了 145 种物质。造成苦味的主要物质可能是氨基酸、二肽和有机酸。
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引用次数: 0
Identification and virulence test of a new pathogen that causes verticillium striping on rapeseed in northwestern China 导致中国西北地区油菜纹枯病的一种新病原体的鉴定和毒力试验
Q3 Agricultural and Biological Sciences Pub Date : 2024-03-01 DOI: 10.1016/j.ocsci.2023.12.005
We Si , Ruisheng Wang , Mingde Wu , Long Yang , Guoqing Li , Jing Zhang

Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province, China, and fungal isolates were obtained from the stems. They were identified based on morphology, molecular features and specific PCR detection. The results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/D3. One of the 10 isolates (HW7-1) was tested for virulence on three species of rapeseed, including B. napus Zhongshuang 9, B. rapa Qingyou 9 and B. juncea Tayou 2 by conidia inoculation of HW7-1 on roots of young seedlings. Control seedlings were inoculated with V. dahliae conidia or water alone. The seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber (20 ​°C). Results suggested that the control seedlings of three cultivars appeared quite healthy, while the seedlings inoculated with HW7-1 turned yellowing leaves, seedling stunting or even death after 22 days post-inoculation. V. longisporum was re-isolated from he yellow leaves, thus fulfilling Koch's postulates. Moreover, compared to the control treatments, inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the stems. To our knowledge, this is the first report of V. longisporum lineage A1/D3 on rapeseed in northwestern China.

从中国青海省湟源县采集了五株带有大量黑色小圆菌丝的油菜茎,并从茎中获得了真菌分离物。根据形态学、分子特征和特异性 PCR 检测对它们进行了鉴定。结果表明,这 10 株真菌属于长孢霉 A1/D3 株系。通过在幼苗根部接种 HW7-1 分生孢子,测试了 10 个分离株中的一个(HW7-1)对 3 种油菜籽的毒力,包括油菜中双 9 号、油菜青优 9 号和油菜大优 2 号。对照组幼苗只接种大丽花病毒分生孢子或水。将这些处理的幼苗移栽到培养混合液中,并在生长室(20 °C)中培养。结果表明,三个栽培品种的对照秧苗看起来相当健康,而接种 HW7-1 的秧苗在接种后 22 天叶片变黄、秧苗发育不良甚至死亡。从黄叶中重新分离出了长孢霉,从而验证了科赫推论。此外,与对照处理相比,接种 HW7-1 会导致 Tayou 2 的开花延迟和种子减产,并在茎上产生小圆菌丝。据我们所知,这是中国西北地区油菜上长孢霉 A1/D3 株系的首次报道。
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引用次数: 0
Effect of defatted flaxseed gum powder addition on the quality of sesame paste 添加脱脂亚麻籽胶粉对芝麻酱质量的影响
Q3 Agricultural and Biological Sciences Pub Date : 2024-02-16 DOI: 10.1016/j.ocsci.2024.01.001
Haichao He , Mingkai Sun , Jiahui Wang , Yan Tang , Yashu Chen , Qianchun Deng , Qingde Huang , Hu Tang

The study explored the influence of defatted flaxseed gum powder (DFGP) on the stability and quality of sesame paste by measuring and analyzing its composition, color, texture, particle size, centrifugal oil separation rate, rheological properties, and microstructure. The results showed that the moisture and polysaccharide content of sesame paste was increased as the DFGP increased. Additionally, the hardness, adhesiveness, and chewiness of the sesame paste was improved, while the presence of particles with small particle size (1–100 ​μm) was decreased. The rate of oil precipitation was reduced by 28.99% when the amount of DFGP was 6%. The sesame paste samples exhibited pseudoplastic behavior, demonstrating shear thinning. As the shear rate increased, the apparent viscosity of sesame paste gradually decreased. Both the storage modulus (G′) and the loss modulus (G″) increased as the shear frequency increased. The microstructure observation revealed that protein and oil were evenly distributed in the sesame paste system, and the addition of DFGP enhanced the bonding between oil and protein. This study can provide valuable references for high-quality sesame paste products in the food industry.

该研究通过测量和分析脱脂亚麻籽胶粉(DFGP)的成分、颜色、质地、粒度、离心分离油率、流变特性和微观结构,探讨了脱脂亚麻籽胶粉(DFGP)对芝麻酱稳定性和质量的影响。结果表明,芝麻酱的水分和多糖含量随着 DFGP 的增加而增加。此外,芝麻酱的硬度、粘附性和咀嚼性也得到了改善,而小粒径(1-100 μm)颗粒的存在则有所减少。当 DFGP 的用量为 6% 时,油的析出率降低了 28.99%。芝麻糊样品表现出假塑性行为,表现出剪切变稀。随着剪切速率的增加,芝麻酱的表观粘度逐渐降低。随着剪切频率的增加,储存模量(G′)和损失模量(G″)都有所增加。微观结构观察表明,蛋白质和油在芝麻糊体系中分布均匀,DFGP 的添加增强了油和蛋白质之间的粘合。这项研究可为食品工业中的高品质芝麻糊产品提供有价值的参考。
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引用次数: 0
Beyond the newsroom: how computer-mediated communication is reshaping journalism in Vietnam 超越新闻编辑室:计算机媒介传播如何重塑越南新闻业
Q3 Agricultural and Biological Sciences Pub Date : 2023-10-16 DOI: 10.1080/01296612.2023.2268435
Pham Chien Thang, Ta Thi Nguyet Trang
AbstractThis study aimed to understand the impact of computer-mediated communication (CMC) tools on journalism in Vietnam by employing a quantitative-methods approach influenced by Social Presence Theory, Media Richness Theory, and Uses and Gratifications Theory. A quantitative survey was conducted with 335 journalists and other stakeholders in newsrooms. The results indicated that social presence significantly affects the adoption and efficacy of CMC tools in this unique cultural context. The study extended the Media Richness Theory by adding a cross-cultural dimension, and the Uses and Gratifications Theory sheds light on the motivations behind tool adoption. This research contributed to theory by adapting established frameworks to a new cultural environment and offers practical guidelines for media organizations in Vietnam undergoing digital transformation. Future research should explore interdisciplinary frameworks incorporating cultural, technological, and psychological factors.Keywords: Computer-mediated communication (CMC)journalismmedia richness theorysocial presence theorydigital transformation Authors’ contributionsIn this study, the first author developed the theoretical framework. The second author analyzed data. They contributed equally to data collection and the writing of this paper.Disclosure statementThere is no conflict of interest regarding the publication of this article. It has not been published anywhere. The authors assert that this manuscript is an original work, with the exception of appropriately cited sources. All the voluntary participants were treated with sincerity, honesty and respect. The researchers considered and followed national ethics policy.Data availability statementhttps://figshare.com/s/fb8a49aee0405e08bb08Additional informationNotes on contributorsPham Chien ThangPham Chien Thang received a Ph.D. from the University of Social Sciences and Humanities, Hanoi, Vietnam in 2019. From 2014 to present, he was a lecturer at the University of Sciences, Thai Nguyen University, Vietnam. In 2020, he became Head of the Department at the Faculty of Journalism and Communication, University of Sciences, Thai Nguyen University, Vietnam. His research interests include the applications of new technology in media, computer-mediated communication, journalism, and social media.Ta Thi Nguyet TrangTa Thi Nguyet Trang received a Ph.D. from the University of Social Sciences and Humanities, Hanoi, Vietnam in 2020. From 2018 to present, she has been a lecturer at the Department of Economics and Management, TNU-International School. Her research interests include marketing communication, new media, economics, and management.
摘要本研究以社会临在理论、媒介丰富性理论、使用与满足理论为基础,运用定量研究方法,探讨越南电脑媒介传播工具对新闻学的影响。对335名记者和新闻编辑室的其他利益相关者进行了定量调查。结果表明,在这种独特的文化背景下,社会存在显著影响CMC工具的采用和功效。该研究通过增加跨文化维度扩展了媒介丰富性理论,而使用和满足理论揭示了工具采用背后的动机。这项研究通过调整现有框架以适应新的文化环境,为理论做出了贡献,并为正在进行数字化转型的越南媒体组织提供了实践指导。未来的研究应探索融合文化、技术和心理因素的跨学科框架。关键词:计算机媒介传播(CMC)新闻媒体丰富度理论社会存在理论数字化转型作者贡献在本研究中,第一作者构建了理论框架。第二作者分析数据。他们对数据收集和本文的撰写都做出了同样的贡献。披露声明本文的发表不存在利益冲突。这本书没有在任何地方发表过。作者声称这篇手稿是原创作品,除了适当引用的来源。所有志愿者都受到真诚、诚实和尊重的对待。研究人员考虑并遵循了国家伦理政策。数据可用性声明https://figshare.com/s/fb8a49aee0405e08bb08Additional informationspham Chien ThangPham Chien Thang于2019年获得越南河内社会科学与人文大学博士学位。2014年至今,任越南太原大学理科大学讲师。2020年,任越南太原大学科学大学新闻与传播学院系主任。他的研究兴趣包括新技术在媒体、计算机媒介传播、新闻学和社交媒体中的应用。Ta Thi nguyen Trang, 2020年毕业于越南河内社会科学与人文大学,获博士学位。2018年至今,任天津工业大学国际学院经济管理系讲师。她的研究兴趣包括营销传播、新媒体、经济学和管理学。
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引用次数: 0
Large scale genetic landscape and population structure of Ethiopian sesame (Sesamum indicum L.) germplasm revealed through molecular marker analysis 通过分子标记分析揭示埃塞俄比亚芝麻(Sesamum indicum L.)种质的大规模遗传景观和种群结构
Q3 Agricultural and Biological Sciences Pub Date : 2023-10-01 DOI: 10.1016/j.ocsci.2023.11.003
Muez Berhe , Jun You , Komivi Dossa , Fetien Abay Abera , Emmanuel Amponsah Adjei , Yanxin Zhang , Linhai Wang

Sesame (Sesamum indicum L.) plays a crucial role in Ethiopian agriculture, serving both subsistence and commercial purposes. However, our understanding of the extensive genetic diversity and population structure of Ethiopian sesame remains limited. To address this knowledge gap, we genotyped 368 Ethiopian sesame germplasms, categorizing into four distinct breeding groups: Accessions, landraces, improved varieties, and wild types, using a comprehensive set of 28 polymorphic markers, including 23 simple sequence repeat (SSR) and five Insertion-Deletion (InDel) markers. These markers ensured robust genomic representation, with at least two markers per linkage group. Our results unveiled substantial genetic diversity, identifying a total of 535 alleles across all accessions. On average, each locus displayed 8.83 alleles, with observed and expected heterozygosity values of 0.30 and 0.36, respectively. Gene Diversity and Polymorphic Information Content (PIC) were recorded at 0.37 and 0.35. The percentage of polymorphic loci varied significantly among breeding groups, ranging from 8.00% to 82.40%, indicating high diversity in accessions (82.4%), moderate diversity in improved varieties (31.20%) and landraces (29.60%), and limited diversity in wild types (8.00). Analysis of Molecular Variance (AMOVA) results emphasized significant genetic differentiation among populations, with substantial diversity (P ​< ​0.001) within each population. Approximately 8% of the entire genetic diversity could be attributed to distinctions among populations, while the larger proportion of genetic diversity (92%) resided within each individual sesame population, showcasing heightened diversity within each group. Our study's findings received support from both Bayesian clustering and Neighbor-joining (NJ) analysis, reaffirming the credibility of our genetic structure insights. Notably, Population structure analysis at its highest Δk value (k ​= ​2) revealed the existence of two primary genetic clusters, further subdivided into four sub-populations at k ​= ​4. Similarly, NJ analysis identified two prominent clusters, each displaying additional sub-clustering. In conclusion, our research provides a comprehensive understanding of genetic groups, subpopulations, and overall diversity within Ethiopian sesame populations. These findings underscore the significant genetic diversity and population structure within Ethiopian sesame germplasm collections. This genetic richness holds promise for breeding and conservation efforts, highlighting the importance of preserving genetic diversity to ensure adaptation to changing environments and meet the needs of farmers and consumers.

芝麻(Sesamum indicum L.)在埃塞俄比亚农业中发挥着至关重要的作用,既可用于生计,也可用于商业目的。然而,我们对埃塞俄比亚芝麻广泛的遗传多样性和种群结构的了解仍然有限。为了填补这一知识空白,我们对 368 个埃塞俄比亚芝麻种质进行了基因分型,分为四个不同的育种组:我们使用一整套 28 个多态性标记,包括 23 个简单序列重复 (SSR) 标记和 5 个插入-缺失 (InDel) 标记,对 368 个埃塞俄比亚芝麻种质进行了基因分型,分为四个不同的育种组:登录组、陆地栽培组、改良品种组和野生类型组。这些标记确保了强大的基因组代表性,每个连接组至少有两个标记。我们的研究结果揭示了大量的遗传多样性,在所有品种中共鉴定出 535 个等位基因。平均而言,每个位点显示 8.83 个等位基因,观察到的杂合度值和预期杂合度值分别为 0.30 和 0.36。基因多样性和多态信息含量(PIC)分别为 0.37 和 0.35。各育种组的多态性位点百分比差异显著,从 8.00% 到 82.40%,表明加入组的多样性较高(82.4%),改良品种(31.20%)和陆生种(29.60%)的多样性适中,而野生型的多样性有限(8.00)。分子方差分析(AMOVA)结果表明,不同种群之间存在显著的遗传差异,每个种群内部都有大量的多样性(P < 0.001)。整个遗传多样性中约有 8% 可归因于种群间的差异,而更大比例的遗传多样性(92%)存在于每个芝麻种群中,显示出每个群体内部的高度多样性。我们的研究结果得到了贝叶斯聚类和邻接(NJ)分析的支持,再次证实了我们的遗传结构见解是可信的。值得注意的是,在最高Δk 值(k = 2)下的种群结构分析显示存在两个主要遗传群,在 k = 4 时进一步细分为四个亚群。同样,NJ 分析也发现了两个突出的聚类,每个聚类又显示出更多的子聚类。总之,我们的研究全面了解了埃塞俄比亚芝麻种群的遗传群体、亚种群和整体多样性。这些发现凸显了埃塞俄比亚芝麻种质资源中重要的遗传多样性和种群结构。这种遗传多样性为育种和保护工作带来了希望,突出了保护遗传多样性以确保适应不断变化的环境并满足农民和消费者需求的重要性。
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引用次数: 0
Correlation and variability analysis of yield and quality related traits in different peanut varieties across various ecological zones of China 中国不同生态区域不同花生品种产量和品质相关性状的相关性和变异性分析
Q3 Agricultural and Biological Sciences Pub Date : 2023-10-01 DOI: 10.1016/j.ocsci.2023.12.001
Zhihui Wang , Yue Zhang , Liying Yan , Yuning Chen , Yanping Kang , Dongxin Huai , Xin Wang , Kede Liu , Huifang Jiang , Yong Lei , Boshou Liao

Peanut cultivation in China spans various ecological zones, each with unique environmental conditions. Identifying suitable peanut varieties for these regions has been challenging due to significant phenotypic variations observed across environments. This study, based on a comprehensive analysis of 256 peanut varieties, selected nine representative varieties (Huayu23, Yuanza9102, Silihong, Wanhua2, Zhonghua6, Zhonghua16, Zhonghua21, Zhonghua215, Zhonghua24) for cultivation in five distinct ecological zones including Chengdu, Hefei, Nanjing, Shijiazhuang, and Wuhan. The yield and quality related phenotypic traits of these varieties were thoroughly assessed, revealing a complex interplay between genetic and environmental factors. Principal component analysis (PCA) effectively distinguished varieties based on yield and quality traits. Strong correlations were observed between specific traits, such as seed size and quality components. The G ​× ​E interaction was evident, as some varieties consistently performed better in certain environments. Varieties with lower coefficient of variation (CV) values exhibited stable trait expression, making them reliable choices for broad cultivation. In contrast, varieties with higher CV values displayed greater sensitivity to environmental fluctuations, potentially due to specific genetic factors. Two high oleic acid varieties, Zhonghua24 and Zhonghua215, demonstrated remarkable stability in oleic acid content across diverse environments, suggesting the presence of genetic mechanisms that buffer against environmental variations. Overall, this study underscores the importance of selecting peanut varieties based on their adaptability and performance in specific ecological zones. These findings provide valuable insights for peanut breeders and farmers, facilitating informed decisions for improved crop production and quality.

中国的花生种植跨越多个生态区,每个生态区都有独特的环境条件。由于在不同环境下观察到的表型差异显著,为这些地区确定合适的花生品种一直是一项挑战。本研究在对 256 个花生品种进行综合分析的基础上,选择了 9 个具有代表性的品种(华玉 23、源杂 9102、四红、万华 2、中华 6、中华 16、中华 21、中华 215、中华 24),在成都、合肥、南京、石家庄和武汉等 5 个不同的生态区进行栽培。对这些品种的产量和品质相关表型性状进行了全面评估,揭示了遗传和环境因素之间复杂的相互作用。主成分分析(PCA)根据产量和品质性状有效地区分了品种。在种子大小和质量成分等特定性状之间观察到了很强的相关性。G × E 的交互作用非常明显,因为有些品种在某些环境中一直表现较好。变异系数(CV)值较低的品种表现出稳定的性状表达,是广泛种植的可靠选择。相比之下,变异系数值较高的品种对环境波动的敏感性更高,这可能是由于特定的遗传因素造成的。中华24和中华215这两个高油酸品种在不同环境下的油酸含量表现出显著的稳定性,表明存在缓冲环境变化的遗传机制。总之,这项研究强调了根据花生在特定生态区的适应性和表现来选择花生品种的重要性。这些发现为花生育种者和农民提供了宝贵的见解,有助于做出明智的决策,提高作物产量和质量。
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引用次数: 0
Proteome-wide identification of S-sulfenylated cysteines response to salt stress in Brassica napus root 全蛋白质组鉴定甘蓝根中 S-亚磺酰化半胱氨酸对盐胁迫的响应
Q3 Agricultural and Biological Sciences Pub Date : 2023-10-01 DOI: 10.1016/j.ocsci.2023.12.002
Qian Qu , Xiaowei Wu , Qing Zhou , Shaoping Lu , Xuan Yao , Liang Guo , Liangqian Yu

Reactive oxygen species (ROS) play a key role in a variety of biological processes, such as the perception of abiotic stress, the integration of different environmental signals, and the activation of stress response networks. Salt stress could induce an increased ROS accumulation in plants, disrupting intracellular redox homeostasis, leading to post-translational modifications (PTMs) of specific proteins, and eventually causing adaptive changes in metabolism. Here, we performed an iodoTMT-based proteomic approach to identify the sulfenylated proteins in B. napus root responsing to salt stress. Totally, 1 348 sulfenylated sites in 751 proteins were identified and these proteins were widely existed in different cell compartments and processes. Our study revealed that proteins with changed abundance and sulfenylation level in B. napus root under salt stress were mainly enriched in the biological processes of ion binding, glycolysis, ATP binding, and oxidative stress response. This study displays a landscape of sulfenylated proteins response to salt stress in B. napus root and provides some theoretical support for further understanding of the molecular mechanisms of redox regulation under salt stress in plants.

活性氧(ROS)在多种生物过程中发挥着关键作用,如感知非生物胁迫、整合不同的环境信号以及激活胁迫响应网络。盐胁迫可诱导植物体内 ROS 积累增加,破坏细胞内氧化还原平衡,导致特定蛋白质的翻译后修饰(PTM),最终引起新陈代谢的适应性变化。在此,我们采用基于碘TMT的蛋白质组学方法鉴定了油菜根系对盐胁迫反应的亚磺酰化蛋白。共鉴定了 751 个蛋白质中的 1 348 个亚硫酰化位点,这些蛋白质广泛存在于不同的细胞区室和过程中。我们的研究发现,在盐胁迫下,油菜根中丰度和亚磺酰化水平发生变化的蛋白质主要富集在离子结合、糖酵解、ATP结合和氧化应激反应等生物过程中。本研究展示了油菜根部亚磺酰化蛋白对盐胁迫的响应图谱,为进一步了解植物盐胁迫下氧化还原调控的分子机制提供了一定的理论支持。
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引用次数: 0
Genome-wide and transcriptome-wide identification of the APX gene family in rapeseed (Brassica napus L.) and their expression features under low temperature stress 油菜(Brassica napus L.)APX 基因家族的全基因组和全转录组鉴定及其在低温胁迫下的表达特征
Q3 Agricultural and Biological Sciences Pub Date : 2023-10-01 DOI: 10.1016/j.ocsci.2023.11.001
Xuan Sun, Guomei Liu, Lin Yao, Chunfang Du

Ascorbate peroxidase (APX) is a crucial H2O2 scavenger that utilizes ascorbic acid as an electron donor and plays a significant role in plant stress resistance. This study aims to identify and characterize the Brassica napus L. APX gene family through genome and transcriptome sequencing, while also revealing their expression profile under low-temperature stress via transcriptome and proteome analysis. The results indicate the presence of 18 genes with three different conserved domains distributed in Brassica napus L., which can be classified into three major branches based on phylogenetic analysis. Eleven members were predicted to have the low-temperature response component (LTR). Most APX genes exhibit up-regulated transcriptional expression under low temperature stress, particularly APX2, APX4, APX12, and APX18. In terms of proteomics data, only six members (APX2, APX4, APX8, APX12, APX17, and APX18) showed temporal specificity in their expression patterns. Therefore, this study provides valuable insights into the complexity of the APX family in the functional characterization of its genes for future research.

抗坏血酸过氧化物酶(APX)是一种重要的 H2O2 清除剂,它利用抗坏血酸作为电子供体,在植物抗逆中发挥着重要作用。本研究旨在通过基因组和转录组测序鉴定甘蓝型油菜 APX 基因家族并确定其特征,同时通过转录组和蛋白质组分析揭示其在低温胁迫下的表达谱。结果表明,18 个基因具有三个不同的保守结构域,分布在甘蓝型油菜(Brassica napus L.)中。有 11 个成员被预测具有低温响应成分(LTR)。大多数 APX 基因在低温胁迫下表现出转录表达上调,尤其是 APX2、APX4、APX12 和 APX18。从蛋白质组学数据来看,只有六个成员(APX2、APX4、APX8、APX12、APX17 和 APX18)的表达模式表现出时间特异性。因此,这项研究为今后研究 APX 家族基因的功能特征提供了有价值的见解。
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引用次数: 0
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Oil Crop Science
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