Oil Crop Science最新文献_第3页

The impact of drought stress on morpho-physiological characteristics of rapeseed cultivars (Brassica napus L.) 干旱胁迫对甘蓝型油菜形态生理特性的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.04.004

Saeid Zamani, Ali Arminian, Batool Zarei, Somayeh Hajinia

The morpho-physiological responses of 15 rapeseed genotypes to drought stress were assessed, revealing strong correlations among various characteristics. Under control conditions, the genotype Angelico fascinated the highest chlorophyll content, while Nilofar had the highest carotenoid content, grain yield, and harvest index (HI). Talaye recorded the highest proline content. Under drought conditions, Nafis maintained the highest plant height, pod number, biomass, and grain yield. Angelico demonstrated the highest chlorophyll and relative water content (RWC). Nima showed the highest carotenoid content, Zarfam excelled in chlorophyll b, and Nafis recorded the highest proline content. Overall, drought stress resulted in a significant reduction in most morpho-physiological traits. In the principal component analysis (PCA), the first two components explained a substantial portion of the variation in both control and drought conditions, facilitating the classification of the genotypes into two distinct groups. Additionally, twelve drought stress indices further categorized the genotypes, with the first two components accounting for the majority of the variation (99.45%). Notably, the Nilofar, Nima, Nafis, and Shirali cultivars were grouped together under both control and drought conditions, exhibiting the highest grain yield. These genotypes could be considered drought-tolerant varieties of this study. The research identified stable and high-performing rapeseed genotypes, which are recommended for future breeding programs.

分析了15个油菜基因型对干旱胁迫的形态生理响应，发现各性状之间存在较强的相关性。在对照条件下，Angelico基因型的叶绿素含量最高，Nilofar基因型的类胡萝卜素含量、籽粒产量和收获指数（HI）最高。塔拉叶脯氨酸含量最高。在干旱条件下，Nafis保持最高的株高、豆荚数、生物量和粮食产量。当归的叶绿素和相对含水量（RWC）最高。Nima的类胡萝卜素含量最高，Zarfam的叶绿素b含量最高，Nafis的脯氨酸含量最高。总体而言，干旱胁迫导致大多数形态生理性状显著降低。在主成分分析（PCA）中，前两个成分解释了对照和干旱条件下的大部分变异，有助于将基因型分为两个不同的群体。此外，12个干旱胁迫指标进一步对基因型进行了分类，其中前两个成分占变异的大部分（99.45%）。值得注意的是，在对照和干旱条件下，Nilofar、Nima、Nafis和Shirali品种组合在一起，表现出最高的粮食产量。这些基因型可以被认为是本研究的耐旱品种。该研究确定了稳定和高性能的油菜籽基因型，为未来的育种计划提供了建议。

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引用次数: 0

ARC microbial inoculants promote peanut growth and yield while suppressing Aspergillus flavus infection ARC微生物接种剂在抑制黄曲霉侵染的同时促进花生生长和产量

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.04.002

Gege Zhang , Ruinan Yang , Xiaodi Ma , Yuxuan Hong , Ming Li , Ling Cheng , Xiaoqian Tang , Qin Han , Qi Zhang , Peiwu Li

Peanuts are important oilseed legume crops that are susceptible to contamination by Aspergillus flavus in soil, leading to serious economic losses. Previously, our research team developed the Aspergillus-Rihizobia coupling (ARC) microbial inoculants and found it can reduce A. flavus abundance in the soil and promote efficient nodulation in peanuts. However, the impact of ARC microbial inoculants on different resistant varieties of A. flavus remains unclear. In this study, we screened peanut varieties that were resistant and susceptible to A. flavus and evaluated their nodulation ability and growth performance after ARC microbial inoculants treatment in the field. The results demonstrated that the nodule number and nitrogenase activity of both varieties significantly increased after ARC microbial inoculants treatment, with the highly susceptible variety AH24 showing a greater increase. For photosynthetic parameters, both varieties also increased after ARC microbial inoculants treatment, but the increase was greater in the moderately resistant variety AH1 than in the highly susceptible variety AH24. Finally, we found that the yield and yield-related traits of the moderately resistant variety AH1 were better than those of the highly susceptible variety AH24. After ARC microbial inoculants treatment, the yield traits of both peanut varieties still increased significantly, but the degree of increase of the moderately resistant variety AH1 was smaller than that of the highly susceptible variety AH24. In addition, the abundance of A. flavus in the rhizosphere soil of the two varieties significantly decreased after ARC microbial inoculants treatment, with no significant difference between the varieties. These results indicated that ARC microbial inoculants exert differential effects on the nodulation and growth of different resistant peanut varieties and have a better effect on highly susceptible varieties. These results provide a solid theoretical basis for the efficient use of ARC microbial inoculants in the field of peanuts in the future.

花生是重要的油籽豆科作物，易受土壤黄曲霉污染，造成严重的经济损失。在此之前，课课组开发了曲霉-根瘤菌耦合（Aspergillus-Rihizobia coupling， ARC）微生物接种剂，发现它可以降低土壤中黄曲霉的丰度，促进花生高效结瘤。然而，ARC微生物接种剂对黄曲霉不同抗性品种的影响尚不清楚。本研究筛选了对黄曲霉抗性和敏感的花生品种，并在田间评价了ARC微生物接种剂处理后的结瘤能力和生长性能。结果表明，ARC微生物接种剂处理后，两个品种的根瘤数和氮酶活性均显著增加，其中高感品种AH24的增加幅度更大。对于光合参数，两个品种在ARC微生物接种剂处理后也有所增加，但中等抗性品种AH1的增加幅度大于高度敏感品种AH24。最后，我们发现中抗性品种AH1的产量和产量相关性状优于高敏感性品种AH24。经ARC微生物接种剂处理后，2个花生品种的产量性状均有显著提高，但中抗性品种AH1的增产幅度小于高感品种AH24。此外，ARC微生物接种剂处理后，两个品种根际土壤黄曲霉丰度均显著降低，但品种间差异不显著。上述结果表明，ARC微生物接种剂对不同抗性花生品种结瘤和生长的影响是不同的，对高感品种效果更好。这些结果为今后ARC微生物接种剂在花生领域的高效利用提供了坚实的理论基础。

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引用次数: 0

Genome-wide analysis and stress-responsive expression profiling of the LEA (late embryogenesis abundant) gene family in wild peanut 野生花生LEA基因家族全基因组分析及应激响应表达谱分析

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2024.09.004

Jiang Huanhuan , Chen Qiangbin , Wang Tong , Chen Gang

Late embryogenesis abundant (LEA) proteins generally accumulate in seeds during the later stages of maturation. Here we studied the LEA genes in two wild peanut species (Arachis duranensis and Arachis ipaensis) in an effort to create a genetic resource for peanut crop improvement. we identified 65 AdLEA and 69 AiLEA genes representing all 8 LEA subfamilies, which were unevenly distributed across 10 peanut chromosomes. The majority of LEA proteins were found to be highly hydrophilic. MEME analysis indicated that LEA gene motifs were conserved within groups, but not between groups. The LEA genes contained a diverse array of stress- and phytohormone-responsive cis-acting elements, with the AdLEA2-20 and AiLEA2-20 genes containing the greatest number of elements. Both AdLEA2-20 and AiLEA2-20 were upregulated in response to cold temperatures, drought, salinity, and abscisic acid exposure, although the dynamics were tissue-dependent. This study lays the foundation for future studies on the LEA gene family and abiotic stress in peanut, and our results will be invaluable for the genetic improvement of peanut by characterizing the genetic resources of wild peanut species.

胚发育后期胚发育后期，种子中富集LEA蛋白。本文对两种野生花生（duranensis和ipaensis）的LEA基因进行了研究，以期为花生作物改良提供遗传资源。我们鉴定出65个AdLEA和69个AiLEA基因，代表了8个LEA亚家族，这些基因不均匀地分布在10条花生染色体上。大多数LEA蛋白被发现是高度亲水的。基因模因分析表明，LEA基因基序组内保守，组间不保守。LEA基因含有多种应激和植物激素响应的顺式作用元件，其中AdLEA2-20和AiLEA2-20基因含有最多的元件。AdLEA2-20和AiLEA2-20在低温、干旱、盐度和脱落酸暴露下均上调，尽管其动态是组织依赖的。本研究为今后花生LEA基因家族和非生物胁迫的研究奠定了基础，对野生花生品种遗传资源的鉴定和花生的遗传改良具有重要意义。

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引用次数: 0

Unraveling the biochemical composition and potential applications of sesame seeds and seed cake: a decade overview 揭示芝麻和种子饼的生化组成和潜在应用：十年综述

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.04.005

Anwesha Mahajan , Vaishali Roy , Paras Sharma

Sesame is the most widely produced oilseed crop with the highest oil content (83%–90%) in Asia, Africa. The seed contains a high amount of protein (19%–35%), dietary fiber (15%–20%), carbohydrate (14%–20%), polyunsaturated fatty acids, and micronutrients. It also contains lignans like sesamol, sesamin, and sesamolin, which protect the oil from oxidative rancidity. The seed also provides seed cake with nutritional value after oil extraction, which is used as a biopolymer in the applications of food packaging, bakery, and confectionary industries, as well as in the preparation of edible and coating films. It can replace commercial expanded polystyrene, which is non-biodegradable in nature. Being the richest source of nutrients, sesame seeds and oilseed cakes have been implemented in different food processing industries. The present review focused on the comprehensive study of biochemical compositions, anti-nutrients, phytochemicals, and antioxidants of sesame seeds and seed cakes and their applications in foods industries.

芝麻是亚洲、非洲种植最广泛的油料作物，含油量最高（83%-90%）。种子含有大量的蛋白质（19%-35%）、膳食纤维（15%-20%）、碳水化合物（14%-20%）、多不饱和脂肪酸和微量营养素。它还含有木脂素，如芝麻酚，芝麻素和芝麻素，可以保护油免受氧化酸败。种子在榨油后还为种子饼提供营养价值，作为生物聚合物应用于食品包装、烘焙和糖果行业，以及制备食用和涂层薄膜。它可以取代商业膨胀聚苯乙烯，在自然界是不可生物降解的。作为最丰富的营养来源，芝麻和油籽饼在不同的食品加工行业中得到了应用。本文对芝麻和芝麻饼的生化成分、抗营养成分、植物化学成分、抗氧化剂及其在食品工业中的应用进行了综述。

引用次数: 0

Development of KASP markers for FT family genes in soybean 大豆FT家族基因KASP标记的开发

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.05.002

Hui Jiang , Xin Xu , Yanhui Sun , Peiguo Wang , Lixin Zhang , Mahmoud Naser , Shi Sun , Jidao Du , Tianfu Han

FLOWERING LOCUS T (FT), a key regulator of photoperiod pathway, plays a pivotal role in modulating flowering time and growth period-related traits in plants. In this study, we identified 10 FT family genes in soybean and examined their functional divergences through sequence alignment analysis. Phylogenetic and amino acid sequence analysis revealed that the 92nd residue and the Segment B domain (sites 129, 131, 134, 135) are the critical determinants of potential functional divergence and activity variation among GmFT. Using the SoyOmics website, we detected natural variations in the genomic and promoter regions of GmFTs, along with numerous haplotypes. By correlating these haplotypes with flowering time, we identified 7 potential KASP markers associated with soybean growth period: GmFT1a-60297360, GmFT1b-60311669, GmFT2a-31695562, GmFT2b-31739433, GmFT3a-4106476, GmFT5a-4078626, and GmFT5b-37490962. All KASP markers exhibited high selection efficiency (97.56%–100.00%) and reliable genotyping accuracy when validated across 82 soybean varieties spanning multiple maturity groups (MGs). This study systematically elucidates the functional divergence of GmFTs and develops efficient KASP markers, providing essential technological support for photoperiod-adaptive breeding and germplasm innovation.

开花位点T （flower LOCUS T， FT）是光周期通路的关键调控因子，在植物开花时间和生长期相关性状的调控中起着关键作用。本研究鉴定了10个大豆FT家族基因，并通过序列比对分析对其功能差异进行了分析。系统发育和氨基酸序列分析表明，第92个残基和B段结构域（129、131、134、135位点）是GmFT潜在功能分化和活性变化的关键决定因素。利用大豆组学网站，我们检测到了GmFTs基因组和启动子区域的自然变异，以及许多单倍型。通过将这些单倍型与开花时间进行关联，我们鉴定出7个与大豆生育期相关的潜在KASP标记：GmFT1a-60297360、GmFT1b-60311669、GmFT2a-31695562、GmFT2b-31739433、GmFT3a-4106476、GmFT5a-4078626和GmFT5b-37490962。在82个大豆品种的多成熟组（mg）中，KASP标记均表现出较高的选择效率（97.56% ~ 100.00%）和可靠的基因分型准确性。该研究系统地阐明了gmft的功能分化，开发了高效的KASP标记，为光周期适应性育种和种质创新提供了必要的技术支持。

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引用次数: 0

The importance of sulphur in oilseed production – A South Asian context 硫在油籽生产中的重要性——南亚背景

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.04.001

V. Visha Kumari , K.A. Gopinath , M.A. Sarath Chandran , B. Sunitha , K. Sriram , M.A. Ansari , M.S. Rao , V.K. Singh

Sulphur (S) is essential for the quality of oilseed crops, ranking as the fourth major nutrient after nitrogen (N), phosphorus (P), and potassium (K). While crops need sulphur in slightly lower amounts than phosphorus, maintaining optimum sulphur levels is crucial for oilseed yield and quality. Factors such as sulphur dynamics, impact of deficiency, application methods, and climate change must be addressed to improve agricultural practices. In South Asian countries like India, Pakistan, and Bangladesh, oilseeds are vital to the agricultural economy, supporting food security, livelihoods, and economic growth. However, climate change, marginal land cultivation, and a shift to more profitable crops like wheat and maize have led to a decline in oilseed productivity. Sulphur is particularly important for vital plant functions, including protein synthesis, chlorophyll formation, and resistance to abiotic stress. Proper management of sulphur can significantly enhance the yield, oil content, and seed quality. Sulphur fertilization improves crop resilience to pests, diseases, and environmental stresses, resulting in healthier plants. To optimize oilseed production, best practices for sulphur management like integrated nutrient management, regular soil testing, and the strategic application of sulphur-enriched fertilizers must be adopted. Despite its significance, many South Asian soils suffer from sulphur deficiency due to inadequate replenishment and reliance on high-analysis fertilizers. By enhancing sulphur availability, oilseed producers can significantly increase crop yields, improve oil content, and promote overall plant health, contributing to the agricultural economy and food security in the region. This manuscript aims to evaluate the critical role of sulphur in enhancing the yield, oil quality, and stress resilience of oilseed crops in South Asia.

硫对油籽作物的品质至关重要，是继氮(N)、磷(P)和钾(K)之后的第四大养分。虽然作物对硫的需要量略低于磷，但保持最佳硫水平对油籽产量和品质至关重要。必须解决诸如硫动态、缺硫影响、施用方法和气候变化等因素，以改善农业实践。在印度、巴基斯坦和孟加拉国等南亚国家，油籽对农业经济至关重要，支持着粮食安全、生计和经济增长。然而，气候变化、边际土地耕作以及转向小麦和玉米等更有利可图的作物，导致油籽产量下降。硫对重要的植物功能尤其重要，包括蛋白质合成、叶绿素形成和对非生物胁迫的抗性。适当的硫管理可以显著提高产量、含油量和种子品质。硫肥可以提高作物对病虫害和环境压力的抵御能力，使植物更健康。为了优化油籽生产，必须采用综合养分管理、定期土壤测试和战略性施用富硫肥料等硫管理的最佳做法。尽管它具有重要意义，但许多南亚土壤由于补充不足和依赖高分析肥料而遭受缺硫。通过提高硫的可用性，油籽生产者可以显著提高作物产量，改善含油量，促进植物整体健康，从而促进该地区的农业经济和粮食安全。本文旨在评价硫在提高南亚油籽作物产量、油质和抗逆性方面的关键作用。

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引用次数: 0

Building MAGIC populations: Novel resources for sunflower trait improvement 构建MAGIC群体：向日葵性状改良的新资源

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-06-01 DOI: 10.1016/j.ocsci.2025.05.001

M. Domínguez , D. Colombo , C. Filippi , E. Ben Guerrero , A. Dillcheneider , A. Corro Molas , J. Montecchia , M. Fass , J. Lavandera , C. Troglia , V. Lia , J. Gonzalez , D. Alvarez , G. Cervigni , N. Paniego

Multiparent advanced generation inter-cross (MAGIC) populations improve the accuracy of quantitative trait loci mapping compared to biparental populations by increasing diversity and reducing linkage disequilibrium between variants. Here we describe the development of two MAGIC populations derived from a cross of 11 founder lines from the INTA (National Institute for Agricultural Technology in Argentina) sunflower breeding program. The founder lines are fertility maintainer inbred lines that exhibit genetic diversity in several traits, including flowering cycle, plant architecture, disease resistance, oil content and oil quality. The crossing scheme applied follows the design of two-way, four-way and eight-way crosses with a modification to increase the number of recombinations and shorten the linkage disequilibrium. Phenotyping a subset of F₂ families and F₅ lines for different traits shows an increase in the diversity of the analyzed traits compared to the parental lines. In addition, we identified lines with transgressive segregation for Sunflower Verticillium Wilt resistance. These results confirm that the MAGIC populations will serve as unique genetic and genomic resources to better characterize the genetics of complex traits and identify potentially superior alleles for sunflower breeding. It will also enrich the gene pool of fertility maintainers (A/B line) of the sunflower germplasm and facilitate the introduction of new breeding methods to select female parents for use in AxR combinations to obtain superior sunflower hybrids.

与双亲本群体相比，多亲本高代杂交群体通过增加多样性和减少变异间连锁不平衡，提高了数量性状位点定位的准确性。在这里，我们描述了两个MAGIC群体的发展，这些群体来自阿根廷国家农业技术研究所（INTA）向日葵育种计划的11个创始系杂交。创始者系是育性维持自交系，在开花周期、植株结构、抗病性、含油量和油质等性状上表现出遗传多样性。所采用的杂交方案遵循双向、四向和八向杂交的设计，并进行了修改，以增加重组次数并缩短连锁不平衡。对F2家族和F5系的不同性状进行表型分析表明，与亲本相比，所分析性状的多样性有所增加。此外，我们还鉴定了向日葵黄萎病抗性的海侵分离系。这些结果证实，MAGIC群体将作为独特的遗传和基因组资源，更好地表征复杂性状的遗传特征，并为向日葵育种找到潜在的优质等位基因。丰富向日葵种质的育性保持系（A/B系）基因库，并为引进新的育种方法选择母本用于AxR组合，获得优质向日葵杂种提供便利。

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引用次数: 0

Plant growth-promoting rhizobacteria on peanut seedlings (Arachis hypogaea L.): Isolation, taxonomical, and functional characterization 花生幼苗上促进植物生长的根瘤菌：分离、分类和功能表征

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-05-13 DOI: 10.1016/j.ocsci.2025.04.003

Ezequiel Darío Bigatton , Ibrahim Ayoub , María Ángeles Castillejo , Carolina Merlo , Carolina Vázquez , Mariela Valeria Archilla , Marina Bruno , María Paula Martín , Romina Paola Pizzolitto , Lucas Esteban Dubini , Enrique Iván Lucini , Ricardo Javier Haro

To improve crop yields, global food production needs sustainable agronomic tools like Plant Growth-Promoting Rhizobacteria (PGPR). Region-adapted PGPR strains are crucial to increasing peanut production. Argentina is the seventh-largest peanut producer, and Córdoba is the main region with 250,000 ha (75% of the total sowing area). This study aimed to isolate, identify, and characterize the biocontrol and growth promotion capacity of PGPR strains belonging to the Bacillus and Pseudomonas genera. The strains were tested against Sclerotinia minor, Sclerotium rolfsii, Fusarium verticillioides, and Aspergillus flavus for biocontrol assays. For growth promotion, pot trials used two peanut cultivars, ASEM 400 INTA and Granoleico, under 40% and 60% field capacity under two water regimes. The isolated strains were Bacillus velezensis, B. subtilis, B. tequilensis, B. safensis, B. altitudinis, and Pseudomonas psychrophila. These strains demonstrated in-vitro phosphorus solubilization, nitrogen fixation, ammonification, nitrification, enzyme releasing, phytohormones production, and high biocontrol capacity of over 75%. SC6 and RI3 (both B. velezensis) and P10 (P. psychrophila) exhibited outstanding performance. They significantly promoted peanut root biomass by more than 50% and leaf area by 30%, with increased chlorophyll content index and leaf relative water content, particularly under water stress conditions (40% field capacity). According to the results, RI3, SC6, and P10 could be classified as PGPR, which supports the results obtained in other field studies with these same microorganisms. Future investigations should prioritize the development of industrial formulations to assess their effectiveness in alternative crops and to incorporate them into other agricultural practices.

为了提高作物产量，全球粮食生产需要可持续的农艺工具，如促进植物生长的根瘤菌（PGPR）。区域适应的PGPR菌株对提高花生产量至关重要。阿根廷是第七大花生生产国，Córdoba是主要种植区域，面积为25万公顷（占总播种面积的75%）。本研究旨在分离、鉴定和表征芽孢杆菌属和假单胞菌属的PGPR菌株的生物防治和促生长能力。对菌株进行了小菌核菌、罗氏菌核菌、黄曲霉、黄萎病镰刀菌的生防试验。为了促进生长，盆栽试验使用ASEM 400 INTA和Granoleico两种花生品种，在两种水分制度下，田间容量分别为40%和60%。分离到的菌株有：韦勒森芽孢杆菌、枯草芽孢杆菌、龙舌兰芽孢杆菌、萨福芽孢杆菌、高原芽孢杆菌和嗜冷假单胞菌。这些菌株在体外表现出溶磷、固氮、氨化、硝化、酶释放、植物激素生产和75%以上的高生物防治能力。SC6和RI3（均为白僵菌）和P10（嗜冷棘菌）表现优异。花生根系生物量显著提高50%以上，叶面积显著提高30%，叶绿素含量指数和叶片相对含水量显著提高，特别是在水分胁迫条件下（40%田间容量）。根据研究结果，RI3、SC6和P10可以归类为PGPR，这支持了在这些微生物的其他野外研究中得到的结果。今后的调查应优先发展工业配方，以评估其在替代作物中的有效性，并将其纳入其他农业实践。

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引用次数: 0

Interactive effect of potassium and zinc application on oil yield of sunflower 钾锌互作对向日葵油料产量的影响

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-03-11 DOI: 10.1016/j.ocsci.2025.03.001

Farid Hellal, Doaa Abou Basha, Saied El Sayed, Hanan Abdelkader

Several initiatives have been made in Egypt to increase the overall production of oil crops, especially sunflower and to close the gap between edible oil production and demand. Field experiments were conducted during the main seasons of 2022 and 2023 at the agricultural research station of National Research Centre, Nubaria, Egypt to investigate the effects of potassium nitrate (0, 20, 40, 60 kg K₂O fed⁻¹) (fed = feddan = 2.4 ha) and zinc sulfate (0, 25, 50, 75 ppm Zn) on growth, oil yield, nutrients components and chemical composition of sunflower. Results indicated that application of potassium nitrate has a promoted effect on chlorophyll content, growth parameters (Head weight, head diameter and 100 seed weight), protein content, oil yield as well as nutrient content under all applied levels. The data also showed that the highest significant increase occurred with the soil application of 40 kg fed⁻¹ potassium nitrate combined with 75 ppm Zn foliar spray. Meanwhile, the lowest values were obtained at the control treatment. Therefore, the current study detected that potassium soil addition at 40 kg K₂O fed⁻¹, besides foliar application with 75 ppm Zn are important to improve the growth, macro-micronutrient, seeds and oil productivity of sunflower crops. Oil yield and nutrient content exhibit a strong positive correlation, with a correlation coefficient of 0.903 for N, 0.94 for P, 0.962 for K and 0.966 for Zn. This indicates that a higher nutrient percentage is associated with higher oil content of sunflower. Study underscores the importance of comprehending the interplay between potassium and zinc treatments in sunflower plants to enhance growth, yield, and oil production effectively. Understanding these relationships can lead to optimized cultivation practices and improved outcomes in sunflower farming.

埃及采取了若干举措，以增加油料作物，特别是向日葵的总产量，并缩小食用油生产与需求之间的差距。在埃及努巴利亚国家研究中心农业研究站，于2022年和2023年主要季节进行田间试验，研究硝酸钾（0、20、40、60 kg K2O投喂−1）（投喂= feddan = 2.4 ha）和硫酸锌（0、25、50、75 ppm Zn）对向日葵生长、产油、营养成分和化学成分的影响。结果表明，在不同施用水平下，硝态钾对水稻叶绿素含量、生长参数（穗重、穗粗和百粒重）、蛋白质含量、产量和养分含量均有促进作用。结果还表明，施用40 kg - 1硝酸钾配以75 ppm Zn叶面喷施的土壤增产效果最显著。而对照处理则最低。因此，本研究发现，除叶面施用75 ppm Zn外，土壤添加40 kg K2O−1钾对向日葵作物的生长、常量微量元素、种子和产油能力都有重要的促进作用。油料产量与养分含量呈较强的正相关，氮、磷、钾、锌的相关系数分别为0.903、0.94、0.962和0.966。这说明向日葵的营养成分百分比越高，其含油量越高。研究强调了了解钾和锌处理对向日葵植株生长、产量和产油量有效促进的重要性。了解这些关系可以优化种植方法并改善向日葵种植的结果。

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引用次数: 0

A reliable, simple and cost-efficient UPLC-vwd method for simultaneously determining tocopherol and tocotrienol in peanut seeds 一种可靠、简单、高效的UPLC-vwd同时测定花生种子中生育酚和生育三烯醇的方法

Q3 Agricultural and Biological Sciences

Oil Crop Science

Pub Date : 2025-03-01 DOI: 10.1016/j.ocsci.2025.02.002

Zekai Tian , Yue Zhang , Chenshuo Sun , Liying Yan , Yanping Kang , Dongxin Huai , Yuning Chen , Xin Wang , Qianqian Wang , Huifang Jiang , Boshou Liao , Zhihui Wang , Yong Lei

Vitamin E is an essential micronutrient that is abundant in peanut seeds. However, the absence of a rapid and reliable method for determining its content has impeded advancements in peanut quality improvement. In this study, we developed an efficient ultra-performance liquid chromatography variable wavelength detector (UPLC-vwd) method for quantifying vitamin E content in peanut seeds, capable of detecting four tocopherols and four tocotrienols. Compared to traditional methods, this approach is simpler, more efficient, and highly accurate. By comparing two sample preparation techniques, it was found that slicing better reflects the true vitamin E content than grinding, as it minimizes losses caused by mechanical pressure. The method demonstrated robust stability and accuracy in both repeatability tests and spiked recovery tests, showing no significant differences compared to the national standard method. The study revealed that the cotyledons of peanuts are the primary storage site for vitamin E, being rich in γ- and α-tocopherols, which together account for over 90°% of the total vitamin E content. In contrast, the vitamin E content in the seed coat is considerably lower. Therefore, the optimized detection method minimizes seed coat interference during sample preparation, ensuring the accuracy of the results. In summary, the UPLC-vwd method developed in this study is a promising tool for determining vitamin E content and supports the improvement of peanut quality.

维生素E是一种必需的微量营养素，在花生种子中含量丰富。然而，缺乏一种快速可靠的测定其含量的方法阻碍了花生品质改善的进展。本研究建立了一种高效的超高效液相色谱变波长检测器（UPLC-vwd）定量花生种子中维生素E含量的方法，可检测4种生育酚和4种生育三烯醇。与传统方法相比，该方法更简单，效率更高，精度更高。通过比较两种样品制备技术，发现切片比研磨更能反映真实的维生素E含量，因为它最大限度地减少了机械压力造成的损失。该方法在重复性试验和加标回收率试验中均表现出良好的稳定性和准确性，与国家标准方法相比无显著差异。研究表明，花生子叶是维生素E的主要储存部位，含有丰富的γ-和α-生育酚，其含量占总维生素E含量的90%以上。相比之下，种皮中的维生素E含量要低得多。因此，优化后的检测方法最大限度地减少了样品制备过程中的种皮干扰，保证了结果的准确性。综上所述，本研究建立的UPLC-vwd方法是一种很有前途的测定花生维生素E含量的工具，为提高花生品质提供了支持。

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